Rw3G007300

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001181
Physical Location & Seq
Reverse (-)
6745340 .. 6749456
4117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G007300.1

Sequence Viewer

Length: 3204 bp
ATGGCTGATAATCGAAATGGAAATAGTAAGCCTACAAAGGCAGCTGGAGCAGGAAATAATCCCTATTCCATTGATTTGAATGTCTTCAGTCAGAGATTGAAGTCCCTCTATTCGCACTGGAATGAGCAGCCTGAGTTGTGGGATTCTAGTGACGTTATTGCGATTGCAACACCGCCGCAATCTGATGAAATCCGGTACCTTAAGTCTTCAGCATTGCAGATGTGGTTGGTTGGGTATGATGTTCCGGATACGATAATGGTGTTCATGAAGAAGGAGATTCATTTCTTGGGTAGCCAGAAGAAGGTGTCTTTTTTCCAAGGTGTGAAGAAGTCTGCCAAAGACGCTGTGGGTGTTGATGTTGTGACGCATGTGAAGGCAAAGAGTGATGATGGCAGTGCTCTGATGGATACTATTTTGGGTGTAATTCGTGCCCAACCAAAGGCTGATGGCCGTGATACTGTTGTTGTAGGAAGGATAGCAAGGGAGGATCCTGAGGGGAATCTTTTGAAATTGTGGTTTGAGAAGCTGAAGAGTGCAACTGTTCAGCTTGGTGATGTAACAAATGGGTTGTCTGACTTGTTTGCTGTCAAGGAGAAAGAGGAACTTGGTAATGTCAAGAAAGCCGCTTACTTAAATACTCTTGTTATGCAGAAAATGGTGGCGAAGCTTGAGAGTGTTATTGATGATGAGAAGAAGGTCACTCACTCTTCTTTGATGGATGAGACAGAGAAACTTATACTAGAACCCTCTAAAGTAGGGCTGAAGCTCAATCCTCAGAATGTTGATATCTGTTACCCTCCAATCTTTCAGAGTGGAGGAAAGTATGATCTCAGACCTGGTGCTGCCAGCAATGATGAGCTTCTTTATTATGATTCTGCAAGTGTGATCATATGTGCTGTTGGATCTCGTTATCACAATTACTGCTCGAATGCTGCCAGGACTTTTTTGATTGATGCTAATCCCCTTCAGAGTAAGGCATATGAGGTTCTTCTCAAGGCCCATGCTGCAGCCATTAGTGAGTTGAAGCCTGACAATAAAGCCAGTGCTGCATATCTAGCAGCACTGGCTGTTGTAGAGAAGGATGCTCCTGAACTATTACCTAATCTGACAAAATCTACGGGGACAGGCATTGGTATTGAGTTCCGTGAATCTGGGTTGAATCTTAATGCAAAAAATGACCGGGTGGTGAAAGCTGGCATGGTCTTTAATGTGTCACTCGGTTTTCATAACTTGCAGAGTGGTATCAGTGATCCAAAGCACCAGAACTTCTCTTTGTTGCTAGCTGACACTGTTGTTGTCAACAATGACAAACCAGAGGTGCTGACGGTAAAAGTTTCTAAAGCTGTTAAAGATGTGGCATACTCCTTTAATGAAGTTGAAGAAGAAAAGCCTGAGAAAGGAAAAGGTCCAGCTAATGGAACAGAGGCCTTGATATCTAAAACCAATCTGAGGTCAGACAATAATGAGAAATCAAAGGAGGAGCTACGAAGGCATCACCAGGCAGAACTTGCCCGCCAGAAAAATGAAGAAACAGCCCGTAGACTTGCTGGTGATGGACATGGATCAGGTAATAATCGTTCCACTGCAAAGTCTTCATCCGATTTAGTGGCCTACAAGAATGTAAATGATCTCAATCCTAAAAGAGATCTGATGATTCAAGTTGACCAGAATAATGAAGCTGTTCTTTTGCCTATATATGGGAGCATGGTCCCCTTTCATGTTGCCACCATAAGGACAGTTAGTGTCCACGCGGAGTCAGCCAACACTAATCAGGCTTGTTATATCAGAATCATCTTCAATGTTCCTGGGACTCCTTTTAGTCCTCATGATACAAACTCACTGAAAAACTTGGGTTCTATATATTTAAAGGAAGTTTCATTTCGCTCCAAGGACACAAGGCACATAAATGAAGTTACACAGGTGATCAAAAACCTTCGGAAGCGAGTTCAGTCCAGGGAGAGTGAGAGAGCTGAGAGGGCTACACTTGTTACTCAGGAGAAACTCCAACTAGCTGGTAACAAATTTAAGCCACTGAGATTGAATGACCTCTGGATTCGACCTGTGTTTGGTGGACGTGGCAGAAAGATTCCAGGTACTCTTGAAGCTCATGCAAATGGGTTTCGTTACTCTACCACCAGACAAGATGAGCGCGTGGATGTAATGTATGGAAATATTAAGCATGCTTTTTTCCAGCCAGCAGAGAATGAGATGATTACTCTCCTTCATTTTCATCTTCACAATCACATAATGGTGGGCAACAAGAAAACCAAGGACGTGCAATTCTATGTGGAGGTTATGGATGTAGTTCAGACCTTGGGAAGTAGTAAGAGATCTGCCTACGACCCAGATGAGATTGAAGAAGAGCAACGAGAGAGGGCTAGGAAGAACAAAGTCAACACTGAATTTCGGACCTTTGTGACTCGGGTACAGGATCTTTGGCAGCAGCCCCAGTTTGAAGGCCTTGAACTTATGTTTGATCAGCCTATGAGGGAGCTTGGTTTCCACGGTGTGCCTTATAAATCCTCAGCATATATCGTCCCTACTACAAATTGCCTGGTTGAACTGATTGAAACTCCTTTTCTTGTTGTCACTCTTGGCGAGATTGAAATTGTGAATCTGGAAAGGGTTGGGCTTGGACAGAAGAATTTTGACCTGGCAATTGTGTTCAAGGATTTTAAAAAAGATGTTCTTCGGATTGATTCGATCCCATCCACAGCACTTGAAGGTCTCAAGGAATGGCTGGACGACACAGAGATCAAGTACTATGAGAGCAGGATGAATCTGTCGTGGAAACCTATACTGAAGACAATTACTGAGGATCCGCAGAGCTTCCTTGATAATGGAGGTTGGGAGTTTTTGAATTTGGAGGGAACGGATTCAGACTCTGAACAGTCTCAGGAGTCAGACCAGGGTTATGAACCATCTGATATGGAATCCGAATCAGAATCGGAGGATGAATCTTCTGATAGTGCGTCACTTGTGGAGTCTGAGGATGATGAGGAGGAAGATTCAGAAGAAGTCTCGGAGGATGAGGAGGGGAAGACATGGGAAGAGTTGGAGAGGGAAGCAAGCAATGCAGACAGGGAGAAAGGCAACGAGTCGGACAGTGAGGAGGACAGGAAGAGAAGGAAGATGAAAGCTTTTGGCAAGGCTCGAGCCCCGCCTGCTGGTAGCATCCCAAAGCGGTCCAAGCTGAGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1067

Amino Acids

119.58

Weight (kDa)

5.55

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FACT-Spt16_Nlob PF14826 24 - 188 5.4e-41 FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24 PF00557 205 - 434 5.3e-29 Metallopeptidase family M24
SPT16 PF08644 552 - 668 3.3e-35 FACT complex subunit (SPT16/CDC68)
PH_SPT16 PF24824 679 - 809 7.9e-55 FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle PF08512 836 - 923 3.5e-22 Histone chaperone Rttp106-like, middle domain
SPT16_C PF21091 963 - 1044 3.9e-17 FACT complex subunit SPT16, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2517
Acc65I GGTACC 1 cut(s) 195
AccB1I GGYRCC 1 cut(s) 195
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccI GTMKAC 1 cut(s) 1540
AccII CGCG 2 cut(s) 1751, 2151
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 8 cut(s) 173, 176, 624, 1513, 1751, 2822, 3161, 3184
AclWI GGATC 9 cut(s) 482, 495, 910, 1244, 1570, 2439, 2698, 2813, 2826
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 4 cut(s) 2021, 2402, 2644, 2860
AcuI CTGAAG 6 cut(s) 70, 192, 548, 782, 950, 2822
AdeI CACNNNGTG 1 cut(s) 2509
AfaI GTAC 4 cut(s) 197, 2095, 2427, 2762
AflII CTTAAG 1 cut(s) 200
AjiI CACGTC 2 cut(s) 2075, 2275
AjnI CCWGG 9 cut(s) 835, 935, 1497, 1804, 1952, 2089, 2553, 2652, 2907
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 4 cut(s) 716, 2732, 2898, 3025
AlwI GGATC 9 cut(s) 482, 495, 910, 1244, 1570, 2439, 2698, 2813, 2826
AlwNI CAGNNNCTG 1 cut(s) 2885
Ama87I CYCGRG 2 cut(s) 2421, 3153
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 5 cut(s) 448, 996, 1425, 1608, 2458
ApoI RAATTY 4 cut(s) 2021, 2402, 2644, 2860
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
Asp700I GAANNNNTTC 3 cut(s) 83, 1680, 2875
Asp718I GGTACC 1 cut(s) 195
AspLEI GCGC 1 cut(s) 2151
AspS9I GGNCC 5 cut(s) 997, 1406, 1708, 2409, 3186
AsuC2I CCSGG 1 cut(s) 1181
AsuHPI GGTGA 5 cut(s) 563, 1198, 1487, 1562, 1933
AsuNHI GCTAGC 1 cut(s) 1279
AvaI CYCGRG 2 cut(s) 2421, 3153
AvaII GGWCC 4 cut(s) 1406, 1708, 2409, 3186
AxyI CCTNAGG 1 cut(s) 492
BaeGI GKGCMC 1 cut(s) 433
BaeI ACNNNNGTAYC 2 cut(s) 447, 480
BamHI GGATCC 2 cut(s) 487, 2818
BanI GGYRCC 1 cut(s) 195
BanII GRGCYC 1 cut(s) 3160
BbsI GAAGAC 5 cut(s) 76, 198, 1584, 2810, 3047
Bbv12I GWGCWC 1 cut(s) 400
BbvCI CCTCAGC 2 cut(s) 2524, 3194
BccI CCATC 7 cut(s) 383, 397, 440, 709, 1547, 2716, 2929
BceAI ACGGC 1 cut(s) 435
BciT130I CCWGG 9 cut(s) 837, 937, 1499, 1806, 1954, 2091, 2555, 2654, 2909
BciVI GTATCC 2 cut(s) 241, 400
BclI TGATCA 3 cut(s) 885, 1923, 2476
BcnI CCSGG 1 cut(s) 1181
BcoDI GTCTC 4 cut(s) 716, 2732, 2898, 3025
BfaI CTAG 6 cut(s) 147, 740, 1055, 1280, 2009, 2379
BfmI CTRYAG 1 cut(s) 1005
BfrI CTTAAG 1 cut(s) 200
BfuI GTATCC 2 cut(s) 241, 400
BglII AGATCT 2 cut(s) 1645, 2330
BmcAI AGTACT 1 cut(s) 2762
Bme18I GGWCC 4 cut(s) 1406, 1708, 2409, 3186
BmeT110I CYCGRG 2 cut(s) 2421, 3153
BmgBI CACGTC 2 cut(s) 2075, 2275
BmgT120I GGNCC 5 cut(s) 997, 1406, 1708, 2409, 3186
BmiI GGNNCC 4 cut(s) 197, 489, 1710, 2820
BmrI ACTGGG 1 cut(s) 2443
BmsI GCATC 4 cut(s) 943, 1072, 1501, 3183
BmtI GCTAGC 1 cut(s) 1283
BmuI ACTGGG 1 cut(s) 2443
BpiI GAAGAC 5 cut(s) 76, 198, 1584, 2810, 3047
BpmI CTGGAG 1 cut(s) 66
Bpu10I CCTNAGC 2 cut(s) 2524, 3194
BpuEI CTTGAG 3 cut(s) 689, 977, 2714
BpuMI CCSGG 1 cut(s) 1181
BsaBI GATNNNNATC 2 cut(s) 957, 1632
BsaI GGTCTC 1 cut(s) 2732
BsaJI CCNNGG 8 cut(s) 316, 1805, 1887, 1953, 2268, 2313, 2503, 2908
BsaWI WCCGGW 2 cut(s) 192, 244
Bse1I ACTGG 4 cut(s) 122, 1041, 1068, 2449
Bse21I CCTNAGG 1 cut(s) 492
Bse3DI GCAATG 3 cut(s) 212, 856, 3079
Bse8I GATNNNNATC 2 cut(s) 957, 1632
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 9 cut(s) 837, 937, 1499, 1806, 1954, 2091, 2555, 2654, 2909
BseDI CCNNGG 8 cut(s) 316, 1805, 1887, 1953, 2268, 2313, 2503, 2908
BseJI GATNNNNATC 2 cut(s) 957, 1632
BseMI GCAATG 3 cut(s) 212, 856, 3079
BseNI ACTGG 4 cut(s) 122, 1041, 1068, 2449
BseRI GAGGAG 4 cut(s) 1493, 3014, 3047, 3125
BseSI GKGCMC 1 cut(s) 433
Bsh1236I CGCG 2 cut(s) 1751, 2151
BshFI GGCC 5 cut(s) 450, 998, 1427, 1610, 2460
BshNI GGYRCC 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 2 cut(s) 2421, 3153
BsiSI CCGG 3 cut(s) 193, 245, 1180
BslFI GGGAC 5 cut(s) 88, 1135, 1694, 1822, 2522
BsmAI GTCTC 4 cut(s) 716, 2732, 2898, 3025
BsmFI GGGAC 5 cut(s) 88, 1135, 1694, 1822, 2522
BsmI GAATGC 1 cut(s) 934
BsnI GGCC 5 cut(s) 450, 998, 1427, 1610, 2460
Bso31I GGTCTC 1 cut(s) 2732
BsoBI CYCGRG 2 cut(s) 2421, 3153
Bsp1286I GDGCHC 3 cut(s) 400, 433, 3160
Bsp13I TCCGGA 1 cut(s) 244
BspACI CCGC 8 cut(s) 173, 176, 624, 1513, 1751, 2822, 3161, 3184
BspANI GGCC 5 cut(s) 450, 998, 1427, 1610, 2460
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 2 cut(s) 1751, 2151
BspHI TCATGA 2 cut(s) 264, 1825
BspLI GGNNCC 4 cut(s) 197, 489, 1710, 2820
BspMAI CTGCAG 1 cut(s) 1009
BspOI GCTAGC 1 cut(s) 1283
BspPI GGATC 9 cut(s) 482, 495, 910, 1244, 1570, 2439, 2698, 2813, 2826
BspQI GCTCTTC 1 cut(s) 2355
BspT107I GGYRCC 1 cut(s) 195
BspTI CTTAAG 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 2732
BsrDI GCAATG 3 cut(s) 212, 856, 3079
BsrI ACTGG 4 cut(s) 122, 1041, 1068, 2449
BssECI CCNNGG 8 cut(s) 316, 1805, 1887, 1953, 2268, 2313, 2503, 2908
BssT1I CCWWGG 4 cut(s) 316, 1887, 2268, 2313
Bst2UI CCWGG 9 cut(s) 837, 937, 1499, 1806, 1954, 2091, 2555, 2654, 2909
Bst4CI ACNGT 8 cut(s) 460, 541, 1291, 1327, 1738, 2507, 2892, 3107
Bst6I CTCTTC 5 cut(s) 524, 712, 2355, 3045, 3116
BstAFI CTTAAG 1 cut(s) 200
BstAPI GCANNNNNTGC 2 cut(s) 1508, 3074
BstC8I GCNNGC 8 cut(s) 847, 1195, 1281, 1513, 2181, 2196, 3070, 3165
BstDSI CCRYGG 1 cut(s) 2503
BstENI CCTNNNNNAGG 1 cut(s) 1395
BstFNI CGCG 2 cut(s) 1751, 2151
BstHHI GCGC 1 cut(s) 2151
BstMAI GTCTC 4 cut(s) 716, 2732, 2898, 3025
BstNI CCWGG 9 cut(s) 837, 937, 1499, 1806, 1954, 2091, 2555, 2654, 2909
BstNSI RCATGY 2 cut(s) 371, 2183
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 1 cut(s) 433
BstUI CGCG 2 cut(s) 1751, 2151
BstV2I GAAGAC 5 cut(s) 76, 198, 1584, 2810, 3047
BstX2I RGATCY 6 cut(s) 487, 902, 1645, 2330, 2431, 2818
BstXI CCANNNNNNTGG 1 cut(s) 2012
BstYI RGATCY 6 cut(s) 487, 902, 1645, 2330, 2431, 2818
Bsu36I CCTNAGG 1 cut(s) 492
BsuI GTATCC 2 cut(s) 241, 400
BsuRI GGCC 5 cut(s) 450, 998, 1427, 1610, 2460
BtgI CCRYGG 1 cut(s) 2503
BtrI CACGTC 2 cut(s) 2075, 2275
BtsI GCAGTG 2 cut(s) 400, 1581
Cac8I GCNNGC 8 cut(s) 847, 1195, 1281, 1513, 2181, 2196, 3070, 3165
CaiI CAGNNNCTG 1 cut(s) 2885
CciI TCATGA 2 cut(s) 264, 1825
CfoI GCGC 1 cut(s) 2151
Cfr13I GGNCC 5 cut(s) 997, 1406, 1708, 2409, 3186
CseI GACGC 3 cut(s) 350, 373, 2961
CsiI ACCWGGT 1 cut(s) 835
Csp6I GTAC 4 cut(s) 196, 2094, 2426, 2761
CspCI CAANNNNNGTGG 4 cut(s) 2019, 2054, 2701, 2736
CviQI GTAC 4 cut(s) 196, 2094, 2426, 2761
DraI TTTAAA 2 cut(s) 1866, 2677
DraIII CACNNNGTG 1 cut(s) 2509
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 5 cut(s) 524, 712, 2355, 3045, 3116
EarI CTCTTC 5 cut(s) 524, 712, 2355, 3045, 3116
Eco130I CCWWGG 4 cut(s) 316, 1887, 2268, 2313
Eco147I AGGCCT 2 cut(s) 1427, 2460
Eco24I GRGCYC 1 cut(s) 3160
Eco31I GGTCTC 1 cut(s) 2732
Eco32I GATATC 2 cut(s) 787, 1434
Eco47I GGWCC 4 cut(s) 1406, 1708, 2409, 3186
Eco57I CTGAAG 6 cut(s) 70, 192, 548, 782, 950, 2822
Eco81I CCTNAGG 1 cut(s) 492
Eco88I CYCGRG 2 cut(s) 2421, 3153
EcoNI CCTNNNNNAGG 1 cut(s) 1395
EcoRII CCWGG 9 cut(s) 835, 935, 1497, 1804, 1952, 2089, 2553, 2652, 2907
EcoRV GATATC 2 cut(s) 787, 1434
EcoT14I CCWWGG 4 cut(s) 316, 1887, 2268, 2313
EcoT38I GRGCYC 1 cut(s) 3160
ErhI CCWWGG 4 cut(s) 316, 1887, 2268, 2313
FalI AAGNNNNNCTT 6 cut(s) 588, 620, 1668, 1700, 2673, 2705
FaqI GGGAC 5 cut(s) 88, 1135, 1694, 1822, 2522
FauI CCCGC 2 cut(s) 1520, 3168
FauNDI CATATG 2 cut(s) 890, 979
FbaI TGATCA 3 cut(s) 885, 1923, 2476
FblI GTMKAC 1 cut(s) 1540
FriOI GRGCYC 1 cut(s) 3160
FspBI CTAG 6 cut(s) 147, 740, 1055, 1280, 2009, 2379
GlaI GCGC 1 cut(s) 2150
GsuI CTGGAG 1 cut(s) 66
HaeIII GGCC 5 cut(s) 450, 998, 1427, 1610, 2460
HapII CCGG 3 cut(s) 193, 245, 1180
HgaI GACGC 3 cut(s) 350, 373, 2961
HhaI GCGC 1 cut(s) 2151
Hin6I GCGC 1 cut(s) 2149
HinP1I GCGC 1 cut(s) 2149
HincII GTYRAC 3 cut(s) 1300, 1663, 2395
HindII GTYRAC 3 cut(s) 1300, 1663, 2395
HindIII AAGCTT 2 cut(s) 665, 3138
HpaII CCGG 3 cut(s) 193, 245, 1180
HphI GGTGA 5 cut(s) 563, 1198, 1487, 1562, 1933
Hpy166II GTNNAC 6 cut(s) 1300, 1541, 1663, 1747, 2072, 2395
Hpy8I GTNNAC 6 cut(s) 1300, 1541, 1663, 1747, 2072, 2395
HpyCH4III ACNGT 8 cut(s) 460, 541, 1291, 1327, 1738, 2507, 2892, 3107
HpyCH4IV ACGT 3 cut(s) 153, 2074, 2274
HpySE526I ACGT 3 cut(s) 153, 2074, 2274
HspAI GCGC 1 cut(s) 2149
Kpn2I TCCGGA 1 cut(s) 244
KpnI GGTACC 1 cut(s) 199
Ksp22I TGATCA 3 cut(s) 885, 1923, 2476
LguI GCTCTTC 1 cut(s) 2355
LmnI GCTCC 6 cut(s) 47, 1090, 1480, 1701, 1889, 2491
LweI GCATC 4 cut(s) 943, 1072, 1501, 3183
MabI ACCWGGT 1 cut(s) 835
MaeI CTAG 6 cut(s) 147, 740, 1055, 1280, 2009, 2379
MaeII ACGT 3 cut(s) 153, 2074, 2274
MfeI CAATTG 1 cut(s) 2658
MflI RGATCY 6 cut(s) 487, 902, 1645, 2330, 2431, 2818
MhlI GDGCHC 3 cut(s) 400, 433, 3160
MlyI GAGTC 7 cut(s) 1763, 1804, 2413, 2876, 2909, 2993, 3107
MmeI TCCRAC 4 cut(s) 880, 2029, 3036, 3081
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 3 cut(s) 83, 1680, 2875
MslI CAYNNNNRTG 4 cut(s) 120, 1905, 2112, 2249
MspA1I CMGCKG 1 cut(s) 44
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 3 cut(s) 193, 245, 1180
MunI CAATTG 1 cut(s) 2658
Mva1269I GAATGC 1 cut(s) 934
MvaI CCWGG 9 cut(s) 837, 937, 1499, 1806, 1954, 2091, 2555, 2654, 2909
MvnI CGCG 2 cut(s) 1751, 2151
NciI CCSGG 1 cut(s) 1181
NdeI CATATG 2 cut(s) 890, 979
NheI GCTAGC 1 cut(s) 1279
NlaIV GGNNCC 4 cut(s) 197, 489, 1710, 2820
NmuCI GTSAC 7 cut(s) 149, 361, 697, 1212, 2416, 2587, 2973
NspI RCATGY 2 cut(s) 371, 2183
PaeI GCATGC 1 cut(s) 2183
PaeR7I CTCGAG 1 cut(s) 3153
PagI TCATGA 2 cut(s) 264, 1825
PceI AGGCCT 2 cut(s) 1427, 2460
PciSI GCTCTTC 1 cut(s) 2355
PctI GAATGC 1 cut(s) 934
PdmI GAANNNNTTC 3 cut(s) 83, 1680, 2875
PflMI CCANNNNNTGG 1 cut(s) 1415
PleI GAGTC 7 cut(s) 1762, 1804, 2413, 2876, 2908, 2992, 3106
PpsI GAGTC 7 cut(s) 1762, 1804, 2413, 2876, 2908, 2992, 3106
PsiI TTATAA 1 cut(s) 2517
Psp6I CCWGG 9 cut(s) 835, 935, 1497, 1804, 1952, 2089, 2553, 2652, 2907
PspGI CCWGG 9 cut(s) 835, 935, 1497, 1804, 1952, 2089, 2553, 2652, 2907
PspN4I GGNNCC 4 cut(s) 197, 489, 1710, 2820
PspPI GGNCC 5 cut(s) 997, 1406, 1708, 2409, 3186
PspXI VCTCGAGB 1 cut(s) 3153
PsrI GAACNNNNNNTAC 2 cut(s) 1561, 1593
PstI CTGCAG 1 cut(s) 1009
PstNI CAGNNNCTG 1 cut(s) 2885
PsuI RGATCY 6 cut(s) 487, 902, 1645, 2330, 2431, 2818
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 4 cut(s) 197, 2095, 2427, 2762
RsaNI GTAC 4 cut(s) 196, 2094, 2426, 2761
RseI CAYNNNNRTG 4 cut(s) 120, 1905, 2112, 2249
SapI GCTCTTC 1 cut(s) 2355
Sau96I GGNCC 5 cut(s) 997, 1406, 1708, 2409, 3186
ScaI AGTACT 1 cut(s) 2762
SchI GAGTC 7 cut(s) 1763, 1804, 2413, 2876, 2909, 2993, 3107
SduI GDGCHC 3 cut(s) 400, 433, 3160
SexAI ACCWGGT 1 cut(s) 835
SfaNI GCATC 4 cut(s) 943, 1072, 1501, 3183
SfcI CTRYAG 1 cut(s) 1005
Sfr274I CTCGAG 1 cut(s) 3153
SinI GGWCC 4 cut(s) 1406, 1708, 2409, 3186
SlaI CTCGAG 1 cut(s) 3153
SmiMI CAYNNNNRTG 4 cut(s) 120, 1905, 2112, 2249
SmlI CTYRAG 5 cut(s) 200, 668, 992, 2729, 3153
SmoI CTYRAG 5 cut(s) 200, 668, 992, 2729, 3153
SphI GCATGC 1 cut(s) 2183
SseBI AGGCCT 2 cut(s) 1427, 2460
SsiI CCGC 8 cut(s) 173, 176, 624, 1513, 1751, 2822, 3161, 3184
SspI AATATT 1 cut(s) 2173
SspMI CTAG 6 cut(s) 147, 740, 1055, 1280, 2009, 2379
StuI AGGCCT 2 cut(s) 1427, 2460
StyI CCWWGG 4 cut(s) 316, 1887, 2268, 2313
TaaI ACNGT 8 cut(s) 460, 541, 1291, 1327, 1738, 2507, 2892, 3107
TaiI ACGT 3 cut(s) 156, 2077, 2277
TaqI TCGA 5 cut(s) 13, 926, 2056, 2702, 3154
TatI WGTACW 1 cut(s) 2760
TauI GCSGC 2 cut(s) 178, 626
TseFI GTSAC 7 cut(s) 149, 361, 697, 1212, 2416, 2587, 2973
Tsp45I GTSAC 7 cut(s) 149, 361, 697, 1212, 2416, 2587, 2973
TspGWI ACGGA 2 cut(s) 1133, 2888
Van91I CCANNNNNTGG 1 cut(s) 1415
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 4 cut(s) 1406, 1708, 2409, 3186
XagI CCTNNNNNAGG 1 cut(s) 1395
XapI RAATTY 4 cut(s) 2021, 2402, 2644, 2860
XceI RCATGY 2 cut(s) 371, 2183
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 3153
XmiI GTMKAC 1 cut(s) 1540
XmnI GAANNNNTTC 3 cut(s) 83, 1680, 2875
XspI CTAG 6 cut(s) 147, 740, 1055, 1280, 2009, 2379
ZrmI AGTACT 1 cut(s) 2762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.