Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0005351

Physical Location & Seq

Genomic Coordinates Forward (+)

23658094 .. 23666422

Gene Structure 8329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG237700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGTCTGGGTTATCTTGCACGAAGCTGACCCTATTCACCGTCGCCGGAGTTGCGATTCAACTCGTCGGCCTCTCTCTCTTCGTCTTCGGCTTCTTCCCCGTCAAGCCAACCCTTCCCGGCCACAGTGGTGCGGAGAGTTTTCGAGCACCCACTTGCAATTCGATTCGAAATGAGAGCCAGAATGATCTGCCTCCTCATCATCTCAGGTCACTCTATAAGGAATTATCTGGAATCCCACCTGCATTTGACAGGCTAATACTAATGGTTATTGATGGTCTTCCAGCTGAGTTTGTTCTTGGCAAGGATGGTGAGCCTCCAAGCAAGGATTTAATGGAGGCTATGCCGTATACTCAGTCATTGCTTGGAAATGGAATGGCAATCGGGTACCATGCAAAAGCCGCACCTCCAACTGTTACAATGCCTCGTTTGAAGGCTATGGTATCTGGAGCAATTGGGGGATTCCTCGATGTGGCTTTGAATTTCAACACACAGGCTATGGTGGATGACAATCTTCTGGGCCAGTTTTTCAAGATTGGTTGGAAAATGGTGATGCTCGGTGATGAAACATGGCTCAAGTTGTTTCCCGGATTGTTTATGAGGCATGATGGAGTCAGCAGTTTCTTCGTCAAAGATACTGTACAAGTGGATCAAAATGTTTCTAGACATCTGGGCCATGAGCTTAGCAGAGATGACTGGAATTTTCTGATTCTTCATTATCTCGGCTTAGATCATGTTGGACATACTGGTGGGCGCAACAGTGCCTTGATGGTCCCAAAACTTACAGAGATGGATGAGGTGGTGAAGATGATTCATATGACTAATATACTGAATCAAAAGAACGATCAAGGACAGACACTCTTGGTCGTAGTAAGTGACCACGGTATGACTGATAGTGGTAATCATGGAGGGTCCTCATATGAAGAAACGGACTCCTTAGCACTATTTATTGGCCTGAAAAATGACGTCTCTGATTATTTGTCGTCTACGCGTAACAATGTTGACCAGGTTGACATCGCACCAACTTTAGCTCTTCTATTAGGTGTGCCAATTCCAAAGAACAATGTTGGTGTTCTAATTCCAGAAATCTTTGGGTATTTGAGTGAAGACCAGCAGCTGAAGGCACTGGAGTTGAATTCCTGGCAATTAGTTAGATTATTGCAAGCACAAATACCTGGTTTATCATGTCGAAATCATCCCTGTGATGGCTCAGGTGGTGATCAAGAGTCCAGAGTAACCAAGTGTTATGGGAGTATAGAAAGGATGTGCTGTTGCTTATATACGAATGCTGCACTTCTCAAAAAGTCCTGGATGTCTAAGGAAGTTTCCAGGTCTAATAGTAGGAAAGACTACACCAGCACTGTTGCAGCATACTATGAGTTTCTGAGAACTGCAAGTGAGTGGCTATCACGCAGAGCAACTGATAAACCTGTAAGCCTGCTTACTCTTGGAATAGCTGCAATGCTCCTATCTTGTCTGATACTGTTAAGCCTTCTGTTTCACATCTACAAAGAAGTCTACACTAGAGAGATGCAATGCCCTTTTGACTTAAAGAGCATCATGCAGACCTGGTGTCTAGAAGAGACATTTATTTTGGGTGTTACATTGATTCTCATTATAAGTATGACATCAAGTTCTTTGGTAGAGGAAGAGCAGTATATTTGGCATTTTATGGCTTCCACATTGCATTTATTACTATTGCGTAAAGCAATTCAAAGTTGTGTACACAGTTTTTCCACGGGACAAAACAAAACAAGTGGTCTTCAAATGAGTTCCATCTGTGTGCTTCTAATTTCTGGGAGGATATTGAGAGGTTGGCACCAAGGAGGCGTGAATTGGACCAATCTTCCTGACATATCTAAGTGGCTTGAGAAGGCTGGGAATGACAATGTAAAATCAGTTCAGCTAGTTGCATGCCTTCTTGTGATTGCCTTAAGCTTATATGCTCTTGTTTTATTTGGATCAAACAGGAATATAGTTCTGGTCATTGGATTTAGCTTTCTTGTGTCTGGGTTGTTGGTTGTGCAAAATATCCTTAAACATCAGGATGGCATGTTTGCCTCATCCAGTTACAGTGCAAATACATTGGTACAGATAATTTATGCAATTCTTGGTGTTTCCACAAGTGGGACAGTTATTGCCTTGCCATGGCTTGTACCTTTTTCAATATCTGAGACTGGCTTGAGTCATGATGTGTACATGTCATCTGCTTCTAGTGAAGTTCAGAATAAGTCACTGCTGATGAAGTTAAGAGAATCTTTATTTCTAATTGGGTGGGCATACATATCCTGCTGGTGTCTTCTGCAGCTTTTGCTCCAACAAACAATTAACTCAATGCCTATACTCCTGCTACATGTGCAAACTTTGGCCAGCATGCTGTATTTTTCTTATAGTGGGTTGCACCACAACCAGTGGGTTGAGGTTTCTGCATTTTACTTTTTGGGAATGGCAGGCCATTTTGCTCTAGGAAACAGCAATTCTCTAGCTACTATTGATGTTGCCGGAGCCTTTATTGTATGTCCTCTGTACCCTTTGCATGGTTCAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016254 GO:0016740 GO:0016772 GO:0016780 GO:0019538 GO:0019637 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0051267 GO:0051377 GO:0071704 GO:0090407 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00563 map00563

Pfam Domains

Protein Families

Phosphodiest

Protein Analysis

848

Amino Acids

93.54

Weight (kDa)

6.23

Isoelectric Point (pI)

42.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphodiest	PF01663	220 - 298	2.4e-07	Type I phosphodiesterase / nucleotide pyrophosphatase
PIGO_PIGG	PF19316	526 - 836	1.6e-11	GPI ethanolamine phosphate transferase membrane region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22530
fragaria_vesca	FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740
malus_domestica	MD15G1313700.v1.1
prunus_persica	Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1
pyrus_communis	pycom15g27700
rosa_chinensis	RchiOBHm_Chr2g0111321
rosa_laevigata	RLG00000017886
rosa_multiflora	Rmu_sc0001363.1_g000002 Rmu_sc0005033.1_g000002 Rmu_ssc0000075.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132500
rosa_rugosa	Rorug02G0172800
rosa_samantha	Rh2AG225900 Rh2BG237700 Rh2CG228900 Rh2DG233400
rosa_wichuraiana	Rw2G017440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1616
AarI	CACCTGC	1 cut(s)	247
AatII	GACGTC	1 cut(s)	966
Acc36I	ACCTGC	1 cut(s)	247
Acc65I	GGTACC	1 cut(s)	384
AccB1I	GGYRCC	2 cut(s)	384, 1815
AccI	GTMKAC	3 cut(s)	347, 983, 1515
AccII	CGCG	1 cut(s)	988
AciI	CCGC	2 cut(s)	131, 399
AclWI	GGATC	2 cut(s)	654, 1966
AcoI	YGGCCR	2 cut(s)	118, 2364
AcsI	RAATTY	3 cut(s)	478, 697, 1132
AcuI	CTGAAG	1 cut(s)	1136
AcyI	GRCGYC	1 cut(s)	963
AfaI	GTAC	7 cut(s)	386, 639, 1722, 2088, 2154, 2195, 2525
AfiI	CCNNNNNNNGG	5 cut(s)	469, 1202, 2124, 2145, 2534
AflII	CTTAAG	1 cut(s)	1930
AflIII	ACRYGT	3 cut(s)	986, 2196, 2350
AgsI	TTSAA	9 cut(s)	59, 430, 478, 484, 529, 1132, 1712, 1763, 2163
AhdI	GACNNNNNGTC	1 cut(s)	1569
AjnI	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
AleI	CACNNNNGTG	1 cut(s)	126
Alw21I	GWGCWC	1 cut(s)	148
Alw26I	GTCTC	3 cut(s)	970, 1574, 2165
AlwI	GGATC	2 cut(s)	654, 1966
AlwNI	CAGNNNCTG	1 cut(s)	1114
AoxI	GGCC	7 cut(s)	67, 118, 517, 670, 949, 2364, 2449
ApeKI	GCWGC	5 cut(s)	1111, 1286, 1364, 1454, 2302
ApoI	RAATTY	3 cut(s)	478, 697, 1132
ArsI	GACNNNNNNTTYG	2 cut(s)	1573, 1605
Asp718I	GGTACC	1 cut(s)	384
AspLEI	GCGC	1 cut(s)	753
AspS9I	GGNCC	5 cut(s)	517, 670, 769, 909, 1836
AsuC2I	CCSGG	2 cut(s)	117, 585
AsuHPI	GGTGA	6 cut(s)	28, 320, 559, 569, 811, 1226
AsuII	TTCGAA	1 cut(s)	166
AvaII	GGWCC	3 cut(s)	769, 909, 1836
BalI	TGGCCA	1 cut(s)	2366
BanI	GGYRCC	2 cut(s)	384, 1815
BbsI	GAAGAC	5 cut(s)	76, 269, 1110, 1751, 2288
Bbv12I	GWGCWC	1 cut(s)	148
BbvI	GCAGC	5 cut(s)	1123, 1273, 1376, 1441, 2314
BccI	CCATC	8 cut(s)	266, 299, 599, 760, 781, 1196, 1781, 2039
BceAI	ACGGC	1 cut(s)	328
BcgI	CGANNNNNNTGC	2 cut(s)	604, 638
BciT130I	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	2 cut(s)	117, 585
BcoDI	GTCTC	3 cut(s)	970, 1574, 2165
BfaI	CTAG	7 cut(s)	660, 1521, 1574, 1904, 2211, 2462, 2480
BfmI	CTRYAG	1 cut(s)	2300
BfrI	CTTAAG	1 cut(s)	1930
BfuAI	ACCTGC	1 cut(s)	247
BisI	GCNGC	6 cut(s)	399, 1112, 1287, 1365, 1455, 2303
BlpI	GCTNAGC	1 cut(s)	680
BlsI	GCNGC	6 cut(s)	400, 1113, 1288, 1366, 1456, 2304
Bme1390I	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
Bme18I	GGWCC	3 cut(s)	769, 909, 1836
BmeRI	GACNNNNNGTC	1 cut(s)	1569
BmgT120I	GGNCC	5 cut(s)	517, 670, 769, 909, 1836
BmiI	GGNNCC	5 cut(s)	386, 771, 910, 1817, 2503
BmrFI	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
BmsI	GCATC	3 cut(s)	540, 1518, 1563
BpiI	GAAGAC	5 cut(s)	76, 269, 1110, 1751, 2288
BpmI	CTGGAG	2 cut(s)	465, 1145
Bpu10I	CCTNAGC	2 cut(s)	934, 1207
Bpu1102I	GCTNAGC	1 cut(s)	680
Bpu14I	TTCGAA	1 cut(s)	166
BpuEI	CTTGAG	3 cut(s)	557, 1886, 2200
BpuMI	CCSGG	2 cut(s)	117, 585
BsaBI	GATNNNNATC	1 cut(s)	507
BsaHI	GRCGYC	1 cut(s)	963
BsaJI	CCNNGG	4 cut(s)	877, 1734, 1819, 2144
Bsc4I	CCNNNNNNNGG	5 cut(s)	469, 1202, 2124, 2145, 2534
Bse1I	ACTGG	7 cut(s)	520, 698, 748, 1128, 2064, 2179, 2407
Bse3DI	GCAATG	4 cut(s)	356, 1464, 1538, 1679
Bse8I	GATNNNNATC	1 cut(s)	507
BseBI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
BseDI	CCNNGG	4 cut(s)	877, 1734, 1819, 2144
BseGI	GGATG	8 cut(s)	310, 508, 796, 1192, 1266, 1314, 2050, 2060
BseJI	GATNNNNATC	1 cut(s)	507
BseLI	CCNNNNNNNGG	5 cut(s)	469, 1202, 2124, 2145, 2534
BseMI	GCAATG	4 cut(s)	356, 1464, 1538, 1679
BseMII	CTCAG	6 cut(s)	217, 276, 365, 1221, 1373, 2160
BseNI	ACTGG	7 cut(s)	520, 698, 748, 1128, 2064, 2179, 2407
BseRI	GAGGAG	1 cut(s)	183
BseXI	GCAGC	5 cut(s)	1123, 1273, 1376, 1441, 2314
BseYI	CCCAGC	1 cut(s)	1874
BsgI	GTGCAG	1 cut(s)	1272
Bsh1236I	CGCG	1 cut(s)	988
BshFI	GGCC	7 cut(s)	69, 120, 519, 672, 951, 2366, 2451
BshNI	GGYRCC	2 cut(s)	384, 1815
BsiHKAI	GWGCWC	1 cut(s)	148
BsiSI	CCGG	4 cut(s)	45, 117, 585, 2499
BslFI	GGGAC	3 cut(s)	755, 1752, 2140
BslI	CCNNNNNNNGG	5 cut(s)	469, 1202, 2124, 2145, 2534
BsmAI	GTCTC	3 cut(s)	970, 1574, 2165
BsmBI	CGTCTC	1 cut(s)	970
BsmFI	GGGAC	3 cut(s)	755, 1752, 2140
BsmI	GAATGC	1 cut(s)	1288
BsnI	GGCC	7 cut(s)	69, 120, 519, 672, 951, 2366, 2451
Bsp119I	TTCGAA	1 cut(s)	166
Bsp1286I	GDGCHC	1 cut(s)	148
Bsp1407I	TGTACA	3 cut(s)	637, 1720, 2193
Bsp143I	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
Bsp1720I	GCTNAGC	1 cut(s)	680
Bsp19I	CCATGG	1 cut(s)	2144
BspACI	CCGC	2 cut(s)	131, 399
BspANI	GGCC	7 cut(s)	69, 120, 519, 672, 951, 2366, 2451
BspCNI	CTCAG	6 cut(s)	216, 277, 364, 1220, 1374, 2161
BspFNI	CGCG	1 cut(s)	988
BspHI	TCATGA	1 cut(s)	2185
BspLI	GGNNCC	5 cut(s)	386, 771, 910, 1817, 2503
BspMAI	CTGCAG	1 cut(s)	2304
BspMI	ACCTGC	1 cut(s)	247
BspPI	GGATC	2 cut(s)	654, 1966
BspQI	GCTCTTC	2 cut(s)	1035, 1641
BspT104I	TTCGAA	1 cut(s)	166
BspT107I	GGYRCC	2 cut(s)	384, 1815
BspTI	CTTAAG	1 cut(s)	1930
BsrDI	GCAATG	4 cut(s)	356, 1464, 1538, 1679
BsrGI	TGTACA	3 cut(s)	637, 1720, 2193
BsrI	ACTGG	7 cut(s)	520, 698, 748, 1128, 2064, 2179, 2407
BssECI	CCNNGG	4 cut(s)	877, 1734, 1819, 2144
BssMI	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
BssNAI	GTATAC	1 cut(s)	348
BssNI	GRCGYC	1 cut(s)	963
BssT1I	CCWWGG	2 cut(s)	1819, 2144
Bst1107I	GTATAC	1 cut(s)	348
Bst2UI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
Bst6I	CTCTTC	4 cut(s)	83, 1035, 1572, 1641
BstACI	GRCGYC	1 cut(s)	963
BstAFI	CTTAAG	1 cut(s)	1930
BstAPI	GCANNNNNTGC	1 cut(s)	2308
BstAUI	TGTACA	3 cut(s)	637, 1720, 2193
BstBI	TTCGAA	1 cut(s)	166
BstC8I	GCNNGC	6 cut(s)	1161, 1436, 1912, 2368, 2372, 2449
BstDSI	CCRYGG	3 cut(s)	877, 1734, 2144
BstF5I	GGATG	8 cut(s)	310, 508, 796, 1192, 1266, 1314, 2050, 2060
BstFNI	CGCG	1 cut(s)	988
BstHHI	GCGC	1 cut(s)	753
BstKTI	GATC	6 cut(s)	187, 649, 730, 844, 1219, 1961
BstMAI	GTCTC	3 cut(s)	970, 1574, 2165
BstMBI	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
BstMWI	GCNNNNNNNGC	4 cut(s)	50, 398, 2308, 2353
BstNI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
BstNSI	RCATGY	5 cut(s)	1914, 2053, 2200, 2354, 2374
BstSCI	CCNGG	8 cut(s)	115, 583, 1002, 1136, 1171, 1304, 1325, 1565
BstSFI	CTRYAG	1 cut(s)	2300
BstUI	CGCG	1 cut(s)	988
BstV1I	GCAGC	5 cut(s)	1123, 1273, 1376, 1441, 2314
BstV2I	GAAGAC	5 cut(s)	76, 269, 1110, 1751, 2288
BstZ17I	GTATAC	1 cut(s)	348
BsuRI	GGCC	7 cut(s)	69, 120, 519, 672, 951, 2366, 2451
BtgI	CCRYGG	3 cut(s)	877, 1734, 2144
BtgZI	GCGATG	1 cut(s)	997
BtsCI	GGATG	8 cut(s)	310, 508, 796, 1192, 1266, 1314, 2050, 2060
BtsI	GCAGTG	1 cut(s)	2231
BtsIMutI	CAGTG	7 cut(s)	130, 763, 1121, 1356, 2077, 2231, 2414
BveI	ACCTGC	1 cut(s)	247
Cac8I	GCNNGC	6 cut(s)	1161, 1436, 1912, 2368, 2372, 2449
CaiI	CAGNNNCTG	1 cut(s)	1114
CciI	TCATGA	1 cut(s)	2185
CfoI	GCGC	1 cut(s)	753
Cfr13I	GGNCC	5 cut(s)	517, 670, 769, 909, 1836
CsiI	ACCWGGT	3 cut(s)	1002, 1171, 1565
Csp6I	GTAC	7 cut(s)	385, 638, 1721, 2087, 2153, 2194, 2524
CspCI	CAANNNNNGTGG	2 cut(s)	1735, 1770
CviQI	GTAC	7 cut(s)	385, 638, 1721, 2087, 2153, 2194, 2524
DpnI	GATC	6 cut(s)	186, 648, 729, 843, 1218, 1960
DpnII	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
DriI	GACNNNNNGTC	1 cut(s)	1569
EaeI	YGGCCR	2 cut(s)	118, 2364
Eam1104I	CTCTTC	4 cut(s)	83, 1035, 1572, 1641
Eam1105I	GACNNNNNGTC	1 cut(s)	1569
EarI	CTCTTC	4 cut(s)	83, 1035, 1572, 1641
Eco130I	CCWWGG	2 cut(s)	1819, 2144
Eco47I	GGWCC	3 cut(s)	769, 909, 1836
Eco57I	CTGAAG	1 cut(s)	1136
EcoO109I	RGGNCCY	1 cut(s)	909
EcoRI	GAATTC	1 cut(s)	1132
EcoRII	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
EcoT14I	CCWWGG	2 cut(s)	1819, 2144
ErhI	CCWWGG	2 cut(s)	1819, 2144
Esp3I	CGTCTC	1 cut(s)	970
FalI	AAGNNNNNCTT	2 cut(s)	2239, 2271
FaqI	GGGAC	3 cut(s)	755, 1752, 2140
FauNDI	CATATG	2 cut(s)	813, 916
FbaI	TGATCA	1 cut(s)	1216
FblI	GTMKAC	3 cut(s)	347, 983, 1515
Fnu4HI	GCNGC	6 cut(s)	399, 1112, 1287, 1365, 1455, 2303
FokI	GGATG	8 cut(s)	317, 515, 803, 1179, 1273, 1321, 2047, 2057
Fsp4HI	GCNGC	6 cut(s)	399, 1112, 1287, 1365, 1455, 2303
FspBI	CTAG	7 cut(s)	660, 1521, 1574, 1904, 2211, 2462, 2480
GlaI	GCGC	1 cut(s)	752
GluI	GCNGC	6 cut(s)	399, 1112, 1287, 1365, 1455, 2303
GsaI	CCCAGC	1 cut(s)	1878
GsuI	CTGGAG	2 cut(s)	465, 1145
HaeIII	GGCC	7 cut(s)	69, 120, 519, 672, 951, 2366, 2451
HapII	CCGG	4 cut(s)	45, 117, 585, 2499
HhaI	GCGC	1 cut(s)	753
Hin1I	GRCGYC	1 cut(s)	963
Hin6I	GCGC	1 cut(s)	751
HinP1I	GCGC	1 cut(s)	751
HincII	GTYRAC	2 cut(s)	1000, 1009
HindII	GTYRAC	2 cut(s)	1000, 1009
HindIII	AAGCTT	1 cut(s)	1933
HpaII	CCGG	4 cut(s)	45, 117, 585, 2499
HphI	GGTGA	6 cut(s)	28, 320, 559, 569, 811, 1226
Hpy166II	GTNNAC	8 cut(s)	348, 984, 1000, 1009, 1516, 1721, 1723, 2194
Hpy188I	TCNGA	7 cut(s)	705, 970, 1383, 1476, 2170, 2223, 2542
Hpy8I	GTNNAC	8 cut(s)	348, 984, 1000, 1009, 1516, 1721, 1723, 2194
Hpy99I	CGWCG	2 cut(s)	44, 68
HpyAV	CCTTC	6 cut(s)	122, 424, 1111, 1499, 1864, 1925
HpyCH4IV	ACGT	1 cut(s)	963
HpyF10VI	GCNNNNNNNGC	4 cut(s)	50, 398, 2308, 2353
HpySE526I	ACGT	1 cut(s)	963
Hsp92I	GRCGYC	1 cut(s)	963
HspAI	GCGC	1 cut(s)	751
KpnI	GGTACC	1 cut(s)	388
Ksp22I	TGATCA	1 cut(s)	1216
Kzo9I	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
LguI	GCTCTTC	2 cut(s)	1035, 1641
LmnI	GCTCC	4 cut(s)	446, 1467, 2316, 2501
Lsp1109I	GCAGC	5 cut(s)	1123, 1273, 1376, 1441, 2314
LweI	GCATC	3 cut(s)	540, 1518, 1563
MabI	ACCWGGT	3 cut(s)	1002, 1171, 1565
MaeI	CTAG	7 cut(s)	660, 1521, 1574, 1904, 2211, 2462, 2480
MaeII	ACGT	1 cut(s)	963
MaeIII	GTNAC	8 cut(s)	207, 412, 872, 989, 1231, 1597, 2066, 2229
MalI	GATC	6 cut(s)	186, 648, 729, 843, 1218, 1960
MboI	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
MfeI	CAATTG	1 cut(s)	450
MhlI	GDGCHC	1 cut(s)	148
MlsI	TGGCCA	1 cut(s)	2366
MluI	ACGCGT	1 cut(s)	986
MluNI	TGGCCA	1 cut(s)	2366
MlyI	GAGTC	4 cut(s)	618, 923, 1232, 2191
MmeI	TCCRAC	4 cut(s)	431, 518, 715, 2338
Mox20I	TGGCCA	1 cut(s)	2366
MscI	TGGCCA	1 cut(s)	2366
MseI	TTAA	8 cut(s)	329, 1484, 1547, 1931, 2034, 2246, 2325, 2545
MslI	CAYNNNNRTG	5 cut(s)	126, 744, 1197, 1778, 2043
Msp20I	TGGCCA	1 cut(s)	2366
MspA1I	CMGCKG	2 cut(s)	284, 1114
MspCI	CTTAAG	1 cut(s)	1930
MspI	CCGG	4 cut(s)	45, 117, 585, 2499
MspR9I	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
MunI	CAATTG	1 cut(s)	450
Mva1269I	GAATGC	1 cut(s)	1288
MvaI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
MvnI	CGCG	1 cut(s)	988
MwoI	GCNNNNNNNGC	4 cut(s)	50, 398, 2308, 2353
NciI	CCSGG	2 cut(s)	117, 585
NcoI	CCATGG	1 cut(s)	2144
NdeI	CATATG	2 cut(s)	813, 916
NdeII	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
NlaIV	GGNNCC	5 cut(s)	386, 771, 910, 1817, 2503
NmeAIII	GCCGAG	1 cut(s)	699
NmuCI	GTSAC	3 cut(s)	207, 872, 2229
NspI	RCATGY	5 cut(s)	1914, 2053, 2200, 2354, 2374
NspV	TTCGAA	1 cut(s)	166
OliI	CACNNNNGTG	1 cut(s)	126
PaeI	GCATGC	2 cut(s)	1914, 2374
PagI	TCATGA	1 cut(s)	2185
PaqCI	CACCTGC	1 cut(s)	247
PciI	ACATGT	2 cut(s)	2196, 2350
PciSI	GCTCTTC	2 cut(s)	1035, 1641
PctI	GAATGC	1 cut(s)	1288
PfeI	GAWTC	9 cut(s)	55, 163, 231, 459, 706, 808, 829, 1606, 2252
PfoI	TCCNGGA	2 cut(s)	583, 1304
PkrI	GCNGC	6 cut(s)	400, 1113, 1288, 1366, 1456, 2304
PleI	GAGTC	4 cut(s)	617, 923, 1231, 2190
PpsI	GAGTC	4 cut(s)	617, 923, 1231, 2190
PpuMI	RGGWCCY	1 cut(s)	909
PscI	ACATGT	2 cut(s)	2196, 2350
PsiI	TTATAA	1 cut(s)	1616
Psp5II	RGGWCCY	1 cut(s)	909
Psp6I	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
PspFI	CCCAGC	1 cut(s)	1874
PspGI	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
PspN4I	GGNNCC	5 cut(s)	386, 771, 910, 1817, 2503
PspPI	GGNCC	5 cut(s)	517, 670, 769, 909, 1836
PspPPI	RGGWCCY	1 cut(s)	909
PsrI	GAACNNNNNNTAC	2 cut(s)	1881, 1913
PstI	CTGCAG	1 cut(s)	2304
PstNI	CAGNNNCTG	1 cut(s)	1114
PvuII	CAGCTG	2 cut(s)	284, 1114
RsaI	GTAC	7 cut(s)	386, 639, 1722, 2088, 2154, 2195, 2525
RsaNI	GTAC	7 cut(s)	385, 638, 1721, 2087, 2153, 2194, 2524
RseI	CAYNNNNRTG	5 cut(s)	126, 744, 1197, 1778, 2043
SapI	GCTCTTC	2 cut(s)	1035, 1641
SaqAI	TTAA	8 cut(s)	329, 1484, 1547, 1931, 2034, 2246, 2325, 2545
SatI	GCNGC	6 cut(s)	399, 1112, 1287, 1365, 1455, 2303
Sau3AI	GATC	6 cut(s)	184, 646, 727, 841, 1216, 1958
Sau96I	GGNCC	5 cut(s)	517, 670, 769, 909, 1836
SchI	GAGTC	4 cut(s)	618, 923, 1232, 2191
ScrFI	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
SduI	GDGCHC	1 cut(s)	148
SexAI	ACCWGGT	3 cut(s)	1002, 1171, 1565
SfaNI	GCATC	3 cut(s)	540, 1518, 1563
SfcI	CTRYAG	1 cut(s)	2300
SfuI	TTCGAA	1 cut(s)	166
SinI	GGWCC	3 cut(s)	769, 909, 1836
SmiMI	CAYNNNNRTG	5 cut(s)	126, 744, 1197, 1778, 2043
SmlI	CTYRAG	4 cut(s)	572, 1865, 1930, 2179
SmoI	CTYRAG	4 cut(s)	572, 1865, 1930, 2179
SphI	GCATGC	2 cut(s)	1914, 2374
SsiI	CCGC	2 cut(s)	131, 399
SspMI	CTAG	7 cut(s)	660, 1521, 1574, 1904, 2211, 2462, 2480
StyD4I	CCNGG	8 cut(s)	115, 583, 1002, 1136, 1171, 1304, 1325, 1565
StyI	CCWWGG	2 cut(s)	1819, 2144
TaiI	ACGT	1 cut(s)	966
TaqI	TCGA	5 cut(s)	142, 161, 166, 465, 1186
TatI	WGTACW	3 cut(s)	637, 1720, 2193
TauI	GCSGC	1 cut(s)	401
TfiI	GAWTC	9 cut(s)	55, 163, 231, 459, 706, 808, 829, 1606, 2252
Tru1I	TTAA	8 cut(s)	329, 1484, 1547, 1931, 2034, 2246, 2325, 2545
Tru9I	TTAA	8 cut(s)	329, 1484, 1547, 1931, 2034, 2246, 2325, 2545
TscAI	CASTG	7 cut(s)	130, 763, 1128, 1363, 2077, 2238, 2414
TseFI	GTSAC	3 cut(s)	207, 872, 2229
TseI	GCWGC	5 cut(s)	1111, 1286, 1364, 1454, 2302
Tsp45I	GTSAC	3 cut(s)	207, 872, 2229
TspDTI	ATGAA	5 cut(s)	576, 701, 800, 933, 2255
TspGWI	ACGGA	1 cut(s)	941
TspRI	CASTG	7 cut(s)	130, 763, 1128, 1363, 2077, 2238, 2414
Vha464I	CTTAAG	1 cut(s)	1930
VpaK11BI	GGWCC	3 cut(s)	769, 909, 1836
XapI	RAATTY	3 cut(s)	478, 697, 1132
XbaI	TCTAGA	2 cut(s)	659, 1573
XceI	RCATGY	5 cut(s)	1914, 2053, 2200, 2354, 2374
XmiI	GTMKAC	3 cut(s)	347, 983, 1515
XspI	CTAG	7 cut(s)	660, 1521, 1574, 1904, 2211, 2462, 2480
ZraI	GACGTC	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.