MD15G1317300.v1.1

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015519
Physical Location & Seq
Reverse (-)
32730766 .. 32735392
4627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1317300.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGCTTTTGAGGCCAGCATTCTTGATGGAACTTGTTCTTACCAAATGGGTATGCGCATTTCTACTGTGCTGTTGGCTCTTCGTTTCTGGAGTGTACTCAGTGGAGGGATTGCGTGGGGACTCTAAAGTGAGAGGGGTGAACTTGGGAGGGTGGTTGGTTGTGGAAGGTTGGATTAAGCCTTCACTTTTTGATGACATTCCCAATGAAGACATGCTGGATGGAACACAGGTACAGCTAAAGTCAGTCACCTTGGAGAAGTACGTCTCTGCTGAAAATGGTGGAGGGATGAATGTCTCAGTTGATAGAGATATTGCATCTTCATGGGAAACCTTTAGGTTATGGAGAGTTTCTGAGTCAGAATTTCAGTTTCGCACCTTGCAAGGACAGTTTCTAACATGTGATGGAGAAGGTTGCTTTTTTTCTGCTGCAGAACCACCTCCAACACTAGGAACCTTTAACATTGAAAGAAATAACAATGGTAGAGTTCACATCAGAACAATAAATGGCACATATTTGCAGGCTACACTGCAAACTCAGCTAACGGCAGACTATCCGGGGAAGCCAGGATGGGAAGATAATGCAGCCACATTTGAGATTACAATTGTTGCTAACACCTTGCATGGAGATTACCAGCTTACAAATGGATATGGATACAGTAAGGCCAAAGATATTCTTAAGAGGCATAGAAACAGTTTCATCACTGTAGGAGATTTCAGTTTTCTGTATAGACATGGAATAAATACTGTGCGGGTTCCTGTTGGCTGGTGGATTGCTTATGATCCTGATCCTCCTGCACCATTTATCGGTGGAACTTTGGAAGCTCTAGATAATGCATTCTCGTGGGCACAAGCCTATAATATTAAGTGCATAATTGATCTTCACGCGGCTCCTGGATCCCAGAACGGGATGGAACATAGTGCTAGTAGAGATGGTTCGACAGACTGGCCTAATCCTGATTACATTTCACAGACACTGCACGTTATAGATTTCCTAGCTTCCAGGTATGCAAGGCATCCTGCTTTGCTGGGAATCGAGCTTTTAAATGAACCATCTGCAGCCGCAGTTCCATTGGATACTTTAGTTTCGTATTACAAACAAGGCTATCAAATTGTTCGGAAATACTCATCAGCCACTTACGTAATAGTTTGCCAAAGAATTGGCAATGCGGACCCATTGGAACTTTATCAGGCTAACATAGACTCACATCATCTAGTGGTGGATTTGCATTATTACAATCTTTTTGATAATTTCTTTGTTAATATGAGTGCTGTGGACAATATGCAATTTATATACAAGAGCAGGGAAGCTCAATTACAGGCCCTCAACAGTGCAAACGGTCCACTTGTTCTTATCGGAGAGTGGGTGAATGAATGGAACGTGACAAATGGCTCTCAGACAGATTATCAAGACTTTGGGAGGGTTCAGTTAGAGGTTTATAATTTGGCTTCCTTCGGATGGGCATACTGGACACTGAAAAACGACAGACCACACTGGGATTTTGAATGGAACATCAAGAACAATTGTCTCCAGTTGGGGAATTCGCCCAAAAACTGGAATGTTAACGGTCTAGTGTTGCTTGGATCAGCATATGTATCATTCTATCTGCATAATATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulase Fascin

Protein Analysis

540

Amino Acids

60.87

Weight (kDa)

5.19

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7910 PF25490 68 - 202 2.8e-53 Domain of unknown function (DUF7910)
Cellulase PF00150 240 - 494 6.3e-21 Cellulase (glycosyl hydrolase family 5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20150 FvH4_1g20150 FvH4_1g20150
malus_domestica MD15G1317300.v1.1
prunus_persica Prupe.6G194500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0112201
rosa_laevigata RLG00000017958
rosa_multiflora Rmu_sc0001386.1_g000002 Rmu_sc0026641.1_g000002
rosa_roxburghii Rroxscaffold_2G00131690
rosa_rugosa Rorug02G0179100 Rorug02G0179200.1
rosa_samantha Rh2AG231300 Rh2BG244300 Rh2CG235700
rosa_wichuraiana Rw2G017930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1437
Acc16I TGCGCA 1 cut(s) 55
AccB7I CCANNNNNTGG 1 cut(s) 1551
AccII CGCG 1 cut(s) 884
AciI CCGC 4 cut(s) 748, 884, 1059, 1166
AclWI GGATC 5 cut(s) 773, 779, 888, 901, 1588
AcsI RAATTY 2 cut(s) 359, 1537
AfaI GTAC 3 cut(s) 95, 231, 260
AfiI CCNNNNNNNGG 5 cut(s) 446, 803, 903, 1455, 1551
AflII CTTAAG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 395
AgsI TTSAA 2 cut(s) 464, 1502
AjnI CCWGG 3 cut(s) 562, 889, 998
AluBI AGCT 7 cut(s) 235, 538, 634, 821, 995, 1036, 1307
AluI AGCT 7 cut(s) 235, 538, 634, 821, 995, 1036, 1307
Alw26I GTCTC 3 cut(s) 268, 298, 1529
AlwI GGATC 5 cut(s) 773, 779, 888, 901, 1588
AlwNI CAGNNNCTG 1 cut(s) 973
AoxI GGCC 4 cut(s) 11, 660, 944, 1317
ApeKI GCWGC 3 cut(s) 425, 581, 1055
ApoI RAATTY 2 cut(s) 359, 1537
Asp700I GAANNNNTTC 1 cut(s) 33
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 3 cut(s) 1168, 1318, 1337
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 3 cut(s) 148, 238, 1375
AvaII GGWCC 2 cut(s) 1168, 1337
BaeGI GKGCMC 1 cut(s) 847
BaeI ACNNNNGTAYC 2 cut(s) 1065, 1098
BamHI GGATCC 1 cut(s) 893
BarI GAAGNNNNNNTAC 2 cut(s) 1296, 1328
BauI CACGAG 1 cut(s) 838
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 412, 593, 1067
BccI CCATC 8 cut(s) 19, 212, 395, 561, 901, 923, 1057, 1449
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 3 cut(s) 564, 891, 1000
BciVI GTATCC 2 cut(s) 644, 1066
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 3 cut(s) 268, 298, 1529
BfaI CTAG 6 cut(s) 446, 824, 921, 992, 1211, 1568
BfmI CTRYAG 3 cut(s) 426, 702, 1053
BfrI CTTAAG 1 cut(s) 674
BfuI GTATCC 2 cut(s) 644, 1066
BisI GCNGC 5 cut(s) 426, 582, 885, 1056, 1059
BlsI GCNGC 5 cut(s) 427, 583, 886, 1057, 1060
Bme1390I CCNGG 4 cut(s) 555, 564, 891, 1000
Bme18I GGWCC 2 cut(s) 1168, 1337
BmgT120I GGNCC 3 cut(s) 1168, 1318, 1337
BmiI GGNNCC 5 cut(s) 451, 753, 888, 895, 1170
BmrFI CCNGG 4 cut(s) 555, 564, 891, 1000
BmrI ACTGGG 1 cut(s) 1501
BmsI GCATC 2 cut(s) 323, 1021
BmuI ACTGGG 1 cut(s) 1501
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 2 cut(s) 108, 1511
BpuMI CCSGG 1 cut(s) 555
BsaAI YACGTR 1 cut(s) 1138
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 249, 554
BsaXI ACNNNNNCTCC 2 cut(s) 1347, 1377
Bsc4I CCNNNNNNNGG 5 cut(s) 446, 803, 903, 1455, 1551
Bse1I ACTGG 5 cut(s) 947, 1469, 1496, 1528, 1556
Bse3DI GCAATG 1 cut(s) 1168
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 3 cut(s) 564, 891, 1000
BseDI CCNNGG 2 cut(s) 249, 554
BseGI GGATG 6 cut(s) 223, 291, 572, 912, 1012, 1460
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 5 cut(s) 446, 803, 903, 1455, 1551
BseMI GCAATG 1 cut(s) 1168
BseMII CTCAG 5 cut(s) 111, 309, 342, 548, 1406
BseNI ACTGG 5 cut(s) 947, 1469, 1496, 1528, 1556
BseSI GKGCMC 1 cut(s) 847
BseXI GCAGC 3 cut(s) 412, 593, 1067
BseYI CCCAGC 1 cut(s) 1024
BsgI GTGCAG 2 cut(s) 777, 959
Bsh1236I CGCG 1 cut(s) 884
BshFI GGCC 4 cut(s) 13, 662, 946, 1319
BsiSI CCGG 1 cut(s) 554
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 5 cut(s) 446, 803, 903, 1455, 1551
BsmAI GTCTC 3 cut(s) 268, 298, 1529
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 2 cut(s) 17, 833
BsnI GGCC 4 cut(s) 13, 662, 946, 1319
Bsp1286I GDGCHC 1 cut(s) 847
Bsp143I GATC 5 cut(s) 778, 784, 874, 893, 1580
BspACI CCGC 4 cut(s) 748, 884, 1059, 1166
BspANI GGCC 4 cut(s) 13, 662, 946, 1319
BspCNI CTCAG 5 cut(s) 110, 308, 343, 547, 1405
BspFNI CGCG 1 cut(s) 884
BspLI GGNNCC 5 cut(s) 451, 753, 888, 895, 1170
BspMAI CTGCAG 2 cut(s) 430, 1057
BspPI GGATC 5 cut(s) 773, 779, 888, 901, 1588
BspQI GCTCTTC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 1168
BsrI ACTGG 5 cut(s) 947, 1469, 1496, 1528, 1556
BssECI CCNNGG 2 cut(s) 249, 554
BssMI GATC 5 cut(s) 778, 784, 874, 893, 1580
BssSI CACGAG 1 cut(s) 838
BssT1I CCWWGG 1 cut(s) 249
Bst2BI CACGAG 1 cut(s) 838
Bst2UI CCWGG 3 cut(s) 564, 891, 1000
Bst4CI ACNGT 9 cut(s) 66, 387, 656, 692, 703, 745, 1328, 1337, 1565
Bst6I CTCTTC 1 cut(s) 83
BstAFI CTTAAG 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 1138
BstC8I GCNNGC 2 cut(s) 15, 519
BstDEI CTNAG 5 cut(s) 97, 295, 351, 534, 1392
BstF5I GGATG 6 cut(s) 223, 291, 572, 912, 1012, 1460
BstFNI CGCG 1 cut(s) 884
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 5 cut(s) 781, 787, 877, 896, 1583
BstMAI GTCTC 3 cut(s) 268, 298, 1529
BstMBI GATC 5 cut(s) 778, 784, 874, 893, 1580
BstMWI GCNNNNNNNGC 2 cut(s) 10, 535
BstNI CCWGG 3 cut(s) 564, 891, 1000
BstNSI RCATGY 2 cut(s) 214, 399
BstSCI CCNGG 4 cut(s) 553, 562, 889, 998
BstSFI CTRYAG 3 cut(s) 426, 702, 1053
BstSLI GKGCMC 1 cut(s) 847
BstSNI TACGTA 1 cut(s) 1138
BstUI CGCG 1 cut(s) 884
BstV1I GCAGC 3 cut(s) 412, 593, 1067
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 893
BstXI CCANNNNNNTGG 1 cut(s) 1157
BstYI RGATCY 1 cut(s) 893
BsuI GTATCC 2 cut(s) 644, 1066
BsuRI GGCC 4 cut(s) 13, 662, 946, 1319
BtsCI GGATG 6 cut(s) 223, 291, 572, 912, 1012, 1460
BtsI GCAGTG 2 cut(s) 524, 971
BtsIMutI CAGTG 7 cut(s) 105, 524, 699, 971, 1333, 1469, 1489
Cac8I GCNNGC 2 cut(s) 15, 519
CaiI CAGNNNCTG 1 cut(s) 973
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 3 cut(s) 1168, 1318, 1337
Csp6I GTAC 3 cut(s) 94, 230, 259
CviAII CATG 5 cut(s) 211, 321, 396, 620, 731
CviQI GTAC 3 cut(s) 94, 230, 259
DdeI CTNAG 5 cut(s) 97, 295, 351, 534, 1392
DpnI GATC 5 cut(s) 780, 786, 876, 895, 1582
DpnII GATC 5 cut(s) 778, 784, 874, 893, 1580
DraI TTTAAA 1 cut(s) 1041
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco105I TACGTA 1 cut(s) 1138
Eco130I CCWWGG 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 1168, 1337
EcoO109I RGGNCCY 1 cut(s) 1318
EcoRI GAATTC 1 cut(s) 1537
EcoRII CCWGG 3 cut(s) 562, 889, 998
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 835
ErhI CCWWGG 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 268
FaeI CATG 5 cut(s) 214, 324, 399, 623, 734
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 131
FatI CATG 5 cut(s) 210, 320, 395, 619, 730
FauI CCCGC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 1588
Fnu4HI GCNGC 5 cut(s) 426, 582, 885, 1056, 1059
FokI GGATG 6 cut(s) 230, 298, 579, 919, 999, 1467
Fsp4HI GCNGC 5 cut(s) 426, 582, 885, 1056, 1059
FspAI RTGCGCAY 1 cut(s) 55
FspBI CTAG 6 cut(s) 446, 824, 921, 992, 1211, 1568
FspI TGCGCA 1 cut(s) 55
GlaI GCGC 1 cut(s) 55
GluI GCNGC 5 cut(s) 426, 582, 885, 1056, 1059
GsaI CCCAGC 1 cut(s) 1028
GsuI CTGGAG 2 cut(s) 108, 1511
HaeIII GGCC 4 cut(s) 13, 662, 946, 1319
HapII CCGG 1 cut(s) 554
HhaI GCGC 1 cut(s) 56
Hin1II CATG 5 cut(s) 214, 324, 399, 623, 734
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 1561
HindII GTYRAC 1 cut(s) 1561
HinfI GANTC 4 cut(s) 119, 353, 1029, 1199
HpaI GTTAAC 1 cut(s) 1561
HpaII CCGG 1 cut(s) 554
HphI GGTGA 3 cut(s) 148, 238, 1375
Hpy166II GTNNAC 6 cut(s) 94, 139, 487, 1273, 1340, 1561
Hpy188I TCNGA 7 cut(s) 352, 358, 494, 1116, 1355, 1395, 1454
Hpy188III TCNNGA 7 cut(s) 22, 87, 782, 824, 953, 1406, 1513
Hpy8I GTNNAC 6 cut(s) 94, 139, 487, 1273, 1340, 1561
HpyAV CCTTC 4 cut(s) 158, 189, 401, 1459
HpyCH4III ACNGT 9 cut(s) 66, 387, 656, 692, 703, 745, 1328, 1337, 1565
HpyCH4IV ACGT 4 cut(s) 261, 978, 1137, 1377
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 535
HpyF3I CTNAG 5 cut(s) 97, 295, 351, 534, 1392
HpySE526I ACGT 4 cut(s) 261, 978, 1137, 1377
Hsp92II CATG 5 cut(s) 214, 324, 399, 623, 734
HspAI GCGC 1 cut(s) 54
KspAI GTTAAC 1 cut(s) 1561
Kzo9I GATC 5 cut(s) 778, 784, 874, 893, 1580
LguI GCTCTTC 1 cut(s) 83
LmnI GCTCC 1 cut(s) 892
Lsp1109I GCAGC 3 cut(s) 412, 593, 1067
LweI GCATC 2 cut(s) 323, 1021
MaeI CTAG 6 cut(s) 446, 824, 921, 992, 1211, 1568
MaeII ACGT 4 cut(s) 261, 978, 1137, 1377
MaeIII GTNAC 2 cut(s) 244, 1378
MalI GATC 5 cut(s) 780, 786, 876, 895, 1582
MboI GATC 5 cut(s) 778, 784, 874, 893, 1580
MboII GAAGA 5 cut(s) 70, 218, 309, 584, 869
MfeI CAATTG 2 cut(s) 600, 1519
MflI RGATCY 1 cut(s) 893
MhlI GDGCHC 1 cut(s) 847
MlyI GAGTC 3 cut(s) 113, 362, 1193
MmeI TCCRAC 2 cut(s) 149, 464
Mph1103I ATGCAT 1 cut(s) 835
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 7 cut(s) 174, 456, 675, 861, 1040, 1257, 1560
MslI CAYNNNNRTG 2 cut(s) 319, 838
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 4 cut(s) 555, 564, 891, 1000
MunI CAATTG 2 cut(s) 600, 1519
Mva1269I GAATGC 2 cut(s) 17, 833
MvaI CCWGG 3 cut(s) 564, 891, 1000
MvnI CGCG 1 cut(s) 884
MwoI GCNNNNNNNGC 2 cut(s) 10, 535
NciI CCSGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 1588
NdeII GATC 5 cut(s) 778, 784, 874, 893, 1580
NlaIII CATG 5 cut(s) 214, 324, 399, 623, 734
NlaIV GGNNCC 5 cut(s) 451, 753, 888, 895, 1170
NmuCI GTSAC 2 cut(s) 244, 1378
NsbI TGCGCA 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 2 cut(s) 214, 399
PciI ACATGT 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 83
PctI GAATGC 2 cut(s) 17, 833
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 1029
PflMI CCANNNNNTGG 1 cut(s) 1551
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 5 cut(s) 427, 583, 886, 1057, 1060
PleI GAGTC 3 cut(s) 113, 361, 1193
PpsI GAGTC 3 cut(s) 113, 361, 1193
Ppu21I YACGTR 1 cut(s) 1138
PscI ACATGT 1 cut(s) 395
PsiI TTATAA 1 cut(s) 1437
Psp6I CCWGG 3 cut(s) 562, 889, 998
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 3 cut(s) 562, 889, 998
PspN4I GGNNCC 5 cut(s) 451, 753, 888, 895, 1170
PspPI GGNCC 3 cut(s) 1168, 1318, 1337
PsrI GAACNNNNNNTAC 2 cut(s) 916, 948
PstI CTGCAG 2 cut(s) 430, 1057
PstNI CAGNNNCTG 1 cut(s) 973
PsuI RGATCY 1 cut(s) 893
RsaI GTAC 3 cut(s) 95, 231, 260
RsaNI GTAC 3 cut(s) 94, 230, 259
RseI CAYNNNNRTG 2 cut(s) 319, 838
SapI GCTCTTC 1 cut(s) 83
SaqAI TTAA 7 cut(s) 174, 456, 675, 861, 1040, 1257, 1560
SatI GCNGC 5 cut(s) 426, 582, 885, 1056, 1059
Sau3AI GATC 5 cut(s) 778, 784, 874, 893, 1580
Sau96I GGNCC 3 cut(s) 1168, 1318, 1337
SchI GAGTC 3 cut(s) 113, 362, 1193
ScrFI CCNGG 4 cut(s) 555, 564, 891, 1000
SduI GDGCHC 1 cut(s) 847
SfaNI GCATC 2 cut(s) 323, 1021
SfcI CTRYAG 3 cut(s) 426, 702, 1053
SinI GGWCC 2 cut(s) 1168, 1337
SmiMI CAYNNNNRTG 2 cut(s) 319, 838
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SnaBI TACGTA 1 cut(s) 1138
SsiI CCGC 4 cut(s) 748, 884, 1059, 1166
SspI AATATT 1 cut(s) 859
SspMI CTAG 6 cut(s) 446, 824, 921, 992, 1211, 1568
StyD4I CCNGG 4 cut(s) 553, 562, 889, 998
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 9 cut(s) 66, 387, 656, 692, 703, 745, 1328, 1337, 1565
TaiI ACGT 4 cut(s) 264, 981, 1140, 1380
TaqI TCGA 2 cut(s) 935, 1032
TatI WGTACW 1 cut(s) 93
TauI GCSGC 2 cut(s) 887, 1061
TfiI GAWTC 1 cut(s) 1029
Tru1I TTAA 7 cut(s) 174, 456, 675, 861, 1040, 1257, 1560
Tru9I TTAA 7 cut(s) 174, 456, 675, 861, 1040, 1257, 1560
TscAI CASTG 7 cut(s) 105, 531, 706, 978, 1333, 1476, 1496
TseFI GTSAC 2 cut(s) 244, 1378
TseI GCWGC 3 cut(s) 425, 581, 1055
Tsp45I GTSAC 2 cut(s) 244, 1378
TspDTI ATGAA 6 cut(s) 219, 302, 309, 685, 1059, 1383
TspRI CASTG 7 cut(s) 105, 531, 706, 978, 1333, 1476, 1496
Van91I CCANNNNNTGG 1 cut(s) 1551
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 1168, 1337
XapI RAATTY 2 cut(s) 359, 1537
XbaI TCTAGA 1 cut(s) 823
XceI RCATGY 2 cut(s) 214, 399
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 33
XspI CTAG 6 cut(s) 446, 824, 921, 992, 1211, 1568
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.