Prupe.6G194500_v2.0.a1

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0015519
Physical Location & Seq
Forward (+)
20165462 .. 20172192
6731 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G194500.1

Sequence Viewer

Length: 1599 bp
ATGGAACTTGTTCTTACCAAATGGGTATGCACATTTCTACTTTGCTCTTGGCTCATCTTCTCCGGAGTATACTCAGTGGAGGGATTGCATGGGGACTCCAAAGTGAGAGGAGTGAACTTGGGAGGATGGTTGGTTGTGGAAGGTTGGATTAAGCCTTCACTTTTTGATGGCATTCCCAATGGAGACATGCTGGATGGAACACAGGTACAACTGAAGTCAGTGACTTTGGAGAAGTATGTCTCTGCAGAGAATGGGGGAGGGATGAACGTCTCGGTTAGTAGAGATGTTGCATCTTCATGGGAAACCTTTACGTTATGGAGAGTTTCTGAATCAGAATTTCAATTCCGCACCTTGCAGGGCCAGTTTCTAACATGCGATGGCGAAGGTTGCTCTTTTTCTGCAACTGCAGAATCACCTTCAACATTAGAAACCTTTTACATTGAAAAATATAACAATGACAGAGTTCACATCAAAACAATGAGTGGTACGTATTTGCAGGCTACAGTGGAAAATCAGCTCATCGCAGACTATCCAGGGAAGCCAGGATGGGATGATAATGCAGCCACATTTGAAATGACCATTGTTTCTAATAATTTGCATGGAGATTACCAGCTGGCAAATGGATATGCACACAATAAGGCCAAAGAGGTTCTTAAGAGACATAGAAACAGTTTCATCACTATAGGAGATTTCAATTTTCTGTATAAACATGGAATAAACACTGTGAGGATTCCTGTTGGCTGGTGGATTGCTTATGATCCTGATCCTCCCGCACCATTTATTGGTGGAACTTTGGAAGCTCTAGATAATGCATTCTCATGGGCACAAGCCTATAATATTAAGTGCATAATTGACCTTCATGCGGCTCCTGGCTCCCAGAACGGGATGGAACATAGTGCTAGTAGAGATGGTTCATCAGACTGGCCTAATCCAGATTACATTTCACAAACACTGCACGTTATAGATTTTCTAGCTTCCAGGTATGCAAGACATCCTGCTCTGCTGGGAATTGAGCTTCTAAATGAACCATCTGCAGCTACAGTTCCGTTGGATATTTTAGTTTCATATTACAATCAAGGCTATCAAACTGTTCGGAAACACTCATCAACAGCTTATGTAATAGTTTGCCAAAGAATTGGCAATGCAGATCCATCGGAAGTTTATCATGCTAACATAGGCTCACGTAATCTAGTGGTGGATTTGCATTATTACAATCTTTTTGATAATTTCTTTGTTAATATGAGCGCTGTGGATAATATACAATTCATATACAAGAGCAGGGAAACTCAATTGGAGGCCCTGAACAGTGCAAATGGTCCACTTGTTTTTATTGGAGAGTGGGTGAATGAGTGGAACGTGACAAGCGGCTCTCAGAAAGATTATCAAGAATTTGGGAGGGTCCAGTTAGAGGTTTATAATGCTGCTTCCTTTGGATGGGCTTACTGGACATTGAAAAATGACAGACCACACTGGGATTTTGAATGGAACATTAGGAACAACTATCTCCAATTGGGTAATTCACCCAAGAAACAGAATGTTAACGGTTTAGTGTTGCTGGGCTTGATGTATGTGTTGTTCTGTCTGCATCATACTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulase Fascin

Protein Analysis

533

Amino Acids

59.96

Weight (kDa)

5.3

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20150 FvH4_1g20150 FvH4_1g20150
malus_domestica MD15G1317300.v1.1
prunus_persica Prupe.6G194500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0112201
rosa_laevigata RLG00000017958
rosa_multiflora Rmu_sc0001386.1_g000002 Rmu_sc0026641.1_g000002
rosa_roxburghii Rroxscaffold_2G00131690
rosa_rugosa Rorug02G0179100 Rorug02G0179200.1
rosa_samantha Rh2AG231300 Rh2BG244300 Rh2CG235700
rosa_wichuraiana Rw2G017930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1416
AccB7I CCANNNNNTGG 1 cut(s) 782
AccI GTMKAC 1 cut(s) 69
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 4 cut(s) 346, 771, 863, 1365
AclWI GGATC 3 cut(s) 752, 758, 1142
AcsI RAATTY 2 cut(s) 335, 1388
AcuI CTGAAG 1 cut(s) 233
AfaI GTAC 2 cut(s) 207, 487
AfeI AGCGCT 1 cut(s) 1246
AfiI CCNNNNNNNGG 5 cut(s) 782, 862, 882, 1408, 1434
AflII CTTAAG 1 cut(s) 653
AgsI TTSAA 7 cut(s) 341, 420, 443, 572, 694, 1453, 1481
AjnI CCWGG 4 cut(s) 532, 541, 868, 977
AluBI AGCT 7 cut(s) 517, 613, 800, 974, 1015, 1037, 1112
AluI AGCT 7 cut(s) 517, 613, 800, 974, 1015, 1037, 1112
Alw26I GTCTC 4 cut(s) 177, 244, 274, 652
AlwI GGATC 3 cut(s) 752, 758, 1142
Aor13HI TCCGGA 1 cut(s) 62
Aor51HI AGCGCT 1 cut(s) 1246
AoxI GGCC 4 cut(s) 358, 639, 923, 1296
ApeKI GCWGC 3 cut(s) 560, 1034, 1421
ApoI RAATTY 2 cut(s) 335, 1388
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 1 cut(s) 1247
AspS9I GGNCC 4 cut(s) 358, 1297, 1316, 1399
AsuHPI GGTGA 3 cut(s) 405, 1354, 1512
AvaII GGWCC 2 cut(s) 1316, 1399
BaeGI GKGCMC 1 cut(s) 826
BbvI GCAGC 3 cut(s) 572, 1046, 1408
BcgI CGANNNNNNTGC 2 cut(s) 1134, 1168
BciT130I CCWGG 4 cut(s) 534, 543, 870, 979
BcoDI GTCTC 4 cut(s) 177, 244, 274, 652
BfaI CTAG 4 cut(s) 803, 900, 971, 1190
BfmI CTRYAG 6 cut(s) 243, 405, 501, 681, 1032, 1038
BfoI RGCGCY 1 cut(s) 1248
BfrI CTTAAG 1 cut(s) 653
BisI GCNGC 5 cut(s) 561, 864, 1035, 1366, 1422
BlsI GCNGC 5 cut(s) 562, 865, 1036, 1367, 1423
Bme1390I CCNGG 4 cut(s) 534, 543, 870, 979
Bme18I GGWCC 2 cut(s) 1316, 1399
BmgT120I GGNCC 4 cut(s) 358, 1297, 1316, 1399
BmiI GGNNCC 3 cut(s) 867, 874, 1400
BmrFI CCNGG 4 cut(s) 534, 543, 870, 979
BmrI ACTGGG 1 cut(s) 1480
BmsI GCATC 2 cut(s) 299, 1594
BmuI ACTGGG 1 cut(s) 1480
BsaAI YACGTR 2 cut(s) 489, 1184
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 1 cut(s) 533
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 1326, 1356
Bsc4I CCNNNNNNNGG 5 cut(s) 782, 862, 882, 1408, 1434
Bse1I ACTGG 5 cut(s) 361, 926, 1402, 1448, 1475
Bse3DI GCAATG 1 cut(s) 1147
Bse8I GATNNNNATC 1 cut(s) 762
BseAI TCCGGA 1 cut(s) 62
BseBI CCWGG 4 cut(s) 534, 543, 870, 979
BseDI CCNNGG 1 cut(s) 533
BseGI GGATG 8 cut(s) 131, 199, 267, 551, 556, 891, 991, 1439
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 5 cut(s) 782, 862, 882, 1408, 1434
BseMI GCAATG 1 cut(s) 1147
BseMII CTCAG 2 cut(s) 87, 1385
BseNI ACTGG 5 cut(s) 361, 926, 1402, 1448, 1475
BseRI GAGGAG 1 cut(s) 123
BseSI GKGCMC 1 cut(s) 826
BseXI GCAGC 3 cut(s) 572, 1046, 1408
BseYI CCCAGC 2 cut(s) 1003, 1555
BsgI GTGCAG 1 cut(s) 938
BshFI GGCC 4 cut(s) 360, 641, 925, 1298
BsiSI CCGG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 5 cut(s) 782, 862, 882, 1408, 1434
BsmAI GTCTC 4 cut(s) 177, 244, 274, 652
BsmBI CGTCTC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 107
BsmI GAATGC 2 cut(s) 171, 812
BsnI GGCC 4 cut(s) 360, 641, 925, 1298
Bsp1286I GDGCHC 1 cut(s) 826
Bsp13I TCCGGA 1 cut(s) 62
Bsp143I GATC 3 cut(s) 757, 763, 1147
BspACI CCGC 4 cut(s) 346, 771, 863, 1365
BspANI GGCC 4 cut(s) 360, 641, 925, 1298
BspCNI CTCAG 2 cut(s) 86, 1384
BspEI TCCGGA 1 cut(s) 62
BspLI GGNNCC 3 cut(s) 867, 874, 1400
BspMAI CTGCAG 3 cut(s) 247, 409, 1036
BspPI GGATC 3 cut(s) 752, 758, 1142
BspTI CTTAAG 1 cut(s) 653
BsrDI GCAATG 1 cut(s) 1147
BsrI ACTGG 5 cut(s) 361, 926, 1402, 1448, 1475
BssECI CCNNGG 1 cut(s) 533
BssMI GATC 3 cut(s) 757, 763, 1147
BssNAI GTATAC 1 cut(s) 70
Bst1107I GTATAC 1 cut(s) 70
Bst2UI CCWGG 4 cut(s) 534, 543, 870, 979
Bst4CI ACNGT 7 cut(s) 505, 671, 724, 1042, 1090, 1307, 1544
BstAFI CTTAAG 1 cut(s) 653
BstBAI YACGTR 2 cut(s) 489, 1184
BstC8I GCNNGC 2 cut(s) 498, 615
BstDEI CTNAG 2 cut(s) 73, 1371
BstF5I GGATG 8 cut(s) 131, 199, 267, 551, 556, 891, 991, 1439
BstH2I RGCGCY 1 cut(s) 1248
BstHHI GCGC 1 cut(s) 1247
BstKTI GATC 3 cut(s) 760, 766, 1150
BstMAI GTCTC 4 cut(s) 177, 244, 274, 652
BstMBI GATC 3 cut(s) 757, 763, 1147
BstMWI GCNNNNNNNGC 1 cut(s) 387
BstNI CCWGG 4 cut(s) 534, 543, 870, 979
BstNSI RCATGY 2 cut(s) 190, 375
BstSCI CCNGG 4 cut(s) 532, 541, 868, 977
BstSFI CTRYAG 6 cut(s) 243, 405, 501, 681, 1032, 1038
BstSLI GKGCMC 1 cut(s) 826
BstSNI TACGTA 1 cut(s) 489
BstV1I GCAGC 3 cut(s) 572, 1046, 1408
BstX2I RGATCY 1 cut(s) 1147
BstXI CCANNNNNNTGG 1 cut(s) 1136
BstYI RGATCY 1 cut(s) 1147
BstZ17I GTATAC 1 cut(s) 70
BsuRI GGCC 4 cut(s) 360, 641, 925, 1298
BtgZI GCGATG 2 cut(s) 390, 505
BtsCI GGATG 8 cut(s) 131, 199, 267, 551, 556, 891, 991, 1439
BtsI GCAGTG 1 cut(s) 950
BtsIMutI CAGTG 7 cut(s) 81, 225, 510, 720, 950, 1312, 1468
Cac8I GCNNGC 2 cut(s) 498, 615
CfoI GCGC 1 cut(s) 1247
Cfr13I GGNCC 4 cut(s) 358, 1297, 1316, 1399
Csp6I GTAC 2 cut(s) 206, 486
CviAII CATG 9 cut(s) 89, 187, 297, 372, 599, 710, 819, 860, 1166
CviQI GTAC 2 cut(s) 206, 486
DdeI CTNAG 2 cut(s) 73, 1371
DpnI GATC 3 cut(s) 759, 765, 1149
DpnII GATC 3 cut(s) 757, 763, 1147
Eco105I TACGTA 1 cut(s) 489
Eco47I GGWCC 2 cut(s) 1316, 1399
Eco47III AGCGCT 1 cut(s) 1246
Eco57I CTGAAG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 1297
EcoRII CCWGG 4 cut(s) 532, 541, 868, 977
EcoT22I ATGCAT 1 cut(s) 814
Esp3I CGTCTC 1 cut(s) 274
FaeI CATG 9 cut(s) 92, 190, 300, 375, 602, 713, 822, 863, 1169
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 1 cut(s) 107
FatI CATG 9 cut(s) 88, 186, 296, 371, 598, 709, 818, 859, 1165
FauI CCCGC 1 cut(s) 778
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 5 cut(s) 561, 864, 1035, 1366, 1422
FokI GGATG 8 cut(s) 138, 206, 274, 558, 563, 898, 978, 1446
Fsp4HI GCNGC 5 cut(s) 561, 864, 1035, 1366, 1422
FspBI CTAG 4 cut(s) 803, 900, 971, 1190
GlaI GCGC 1 cut(s) 1246
GluI GCNGC 5 cut(s) 561, 864, 1035, 1366, 1422
GsaI CCCAGC 2 cut(s) 1007, 1559
HaeII RGCGCY 1 cut(s) 1248
HaeIII GGCC 4 cut(s) 360, 641, 925, 1298
HapII CCGG 1 cut(s) 63
HhaI GCGC 1 cut(s) 1247
Hin1II CATG 9 cut(s) 92, 190, 300, 375, 602, 713, 822, 863, 1169
Hin6I GCGC 1 cut(s) 1245
HinP1I GCGC 1 cut(s) 1245
HincII GTYRAC 1 cut(s) 1540
HindII GTYRAC 1 cut(s) 1540
HinfI GANTC 4 cut(s) 95, 329, 410, 730
HpaI GTTAAC 1 cut(s) 1540
HpaII CCGG 1 cut(s) 63
HphI GGTGA 3 cut(s) 405, 1354, 1512
Hpy166II GTNNAC 5 cut(s) 70, 115, 466, 1319, 1540
Hpy188I TCNGA 6 cut(s) 328, 334, 919, 1095, 1156, 1374
Hpy188III TCNNGA 5 cut(s) 63, 761, 803, 932, 1385
Hpy8I GTNNAC 5 cut(s) 70, 115, 466, 1319, 1540
HpyAV CCTTC 5 cut(s) 134, 165, 377, 426, 866
HpyCH4III ACNGT 7 cut(s) 505, 671, 724, 1042, 1090, 1307, 1544
HpyCH4IV ACGT 6 cut(s) 267, 311, 488, 957, 1183, 1356
HpyF10VI GCNNNNNNNGC 1 cut(s) 387
HpyF3I CTNAG 2 cut(s) 73, 1371
HpySE526I ACGT 6 cut(s) 267, 311, 488, 957, 1183, 1356
Hsp92II CATG 9 cut(s) 92, 190, 300, 375, 602, 713, 822, 863, 1169
HspAI GCGC 1 cut(s) 1245
Kpn2I TCCGGA 1 cut(s) 62
KspAI GTTAAC 1 cut(s) 1540
Kzo9I GATC 3 cut(s) 757, 763, 1147
LmnI GCTCC 2 cut(s) 871, 878
Lsp1109I GCAGC 3 cut(s) 572, 1046, 1408
LweI GCATC 2 cut(s) 299, 1594
MaeI CTAG 4 cut(s) 803, 900, 971, 1190
MaeII ACGT 6 cut(s) 267, 311, 488, 957, 1183, 1356
MaeIII GTNAC 2 cut(s) 220, 1357
MalI GATC 3 cut(s) 759, 765, 1149
MboI GATC 3 cut(s) 757, 763, 1147
MboII GAAGA 2 cut(s) 49, 285
MfeI CAATTG 2 cut(s) 1289, 1508
MflI RGATCY 1 cut(s) 1147
MhlI GDGCHC 1 cut(s) 826
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 125, 1029
Mph1103I ATGCAT 1 cut(s) 814
MroI TCCGGA 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 5 cut(s) 150, 654, 840, 1236, 1539
MslI CAYNNNNRTG 2 cut(s) 295, 817
MspA1I CMGCKG 1 cut(s) 613
MspCI CTTAAG 1 cut(s) 653
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 4 cut(s) 534, 543, 870, 979
MunI CAATTG 2 cut(s) 1289, 1508
Mva1269I GAATGC 2 cut(s) 171, 812
MvaI CCWGG 4 cut(s) 534, 543, 870, 979
MwoI GCNNNNNNNGC 1 cut(s) 387
NdeII GATC 3 cut(s) 757, 763, 1147
NlaIII CATG 9 cut(s) 92, 190, 300, 375, 602, 713, 822, 863, 1169
NlaIV GGNNCC 3 cut(s) 867, 874, 1400
NmuCI GTSAC 2 cut(s) 220, 1357
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 2 cut(s) 190, 375
PctI GAATGC 2 cut(s) 171, 812
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 329, 410, 730
PflMI CCANNNNNTGG 1 cut(s) 782
PkrI GCNGC 5 cut(s) 562, 865, 1036, 1367, 1423
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Ppu21I YACGTR 2 cut(s) 489, 1184
PsiI TTATAA 1 cut(s) 1416
Psp6I CCWGG 4 cut(s) 532, 541, 868, 977
PspFI CCCAGC 2 cut(s) 1003, 1555
PspGI CCWGG 4 cut(s) 532, 541, 868, 977
PspN4I GGNNCC 3 cut(s) 867, 874, 1400
PspPI GGNCC 4 cut(s) 358, 1297, 1316, 1399
PsrI GAACNNNNNNTAC 4 cut(s) 895, 927, 1559, 1591
PstI CTGCAG 3 cut(s) 247, 409, 1036
PsuI RGATCY 1 cut(s) 1147
PvuII CAGCTG 1 cut(s) 613
RsaI GTAC 2 cut(s) 207, 487
RsaNI GTAC 2 cut(s) 206, 486
RseI CAYNNNNRTG 2 cut(s) 295, 817
SaqAI TTAA 5 cut(s) 150, 654, 840, 1236, 1539
SatI GCNGC 5 cut(s) 561, 864, 1035, 1366, 1422
Sau3AI GATC 3 cut(s) 757, 763, 1147
Sau96I GGNCC 4 cut(s) 358, 1297, 1316, 1399
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 4 cut(s) 534, 543, 870, 979
SduI GDGCHC 1 cut(s) 826
SfaNI GCATC 2 cut(s) 299, 1594
SfcI CTRYAG 6 cut(s) 243, 405, 501, 681, 1032, 1038
SinI GGWCC 2 cut(s) 1316, 1399
SmiMI CAYNNNNRTG 2 cut(s) 295, 817
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SnaBI TACGTA 1 cut(s) 489
SsiI CCGC 4 cut(s) 346, 771, 863, 1365
SspI AATATT 1 cut(s) 838
SspMI CTAG 4 cut(s) 803, 900, 971, 1190
StyD4I CCNGG 4 cut(s) 532, 541, 868, 977
TaaI ACNGT 7 cut(s) 505, 671, 724, 1042, 1090, 1307, 1544
TaiI ACGT 6 cut(s) 270, 314, 491, 960, 1186, 1359
TauI GCSGC 2 cut(s) 866, 1368
TfiI GAWTC 3 cut(s) 329, 410, 730
Tru1I TTAA 5 cut(s) 150, 654, 840, 1236, 1539
Tru9I TTAA 5 cut(s) 150, 654, 840, 1236, 1539
TscAI CASTG 7 cut(s) 81, 225, 510, 727, 957, 1312, 1475
TseFI GTSAC 2 cut(s) 220, 1357
TseI GCWGC 3 cut(s) 560, 1034, 1421
Tsp45I GTSAC 2 cut(s) 220, 1357
TspDTI ATGAA 8 cut(s) 278, 285, 664, 848, 903, 1038, 1053, 1255
TspGWI ACGGA 1 cut(s) 1035
TspRI CASTG 7 cut(s) 81, 225, 510, 727, 957, 1312, 1475
Van91I CCANNNNNTGG 1 cut(s) 782
Vha464I CTTAAG 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 1316, 1399
XapI RAATTY 2 cut(s) 335, 1388
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 2 cut(s) 190, 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 4 cut(s) 803, 900, 971, 1190
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.