MD15G1440600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016920
Physical Location & Seq
Forward (+)
54047620 .. 54049524
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1440600.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGTTTGCCTACAAACCCAAATCCCTCTTCCCATCTCTTCCAAAACACATTTCAAATTCCCTACTTTCTCACTCCTCCTCCTCCTCCTCACTTGTCTCTGCACTCCCACCGCGCCCTTCCCTCCGCCAAACCAAGCAAGCCCATGCTCACATCATCGTCTCCGGCCACGCCGCCTACTCTCCCCTCCTTTCCCACCTCCTCTGCCTCCTTTCTCTCTCCCCAACTACTCCATTTCACTACTCCCTCTCTCTCTACCACTCCATCAAATACCCATCTGTTTTCGCCACCAATAACATGATCCGCTGCTTCGCAAAGAGCGATTCGCCTCTCCTTTCCCTGCTTCTCTTTTCCTGCTCCATCCTGCGATCCTGCGTCAAGCCCAACAACCATACTTTTACTTTTCTGCTTCAAGCTTGCAGCAGGGCTATGGCGCTCACTGAAGGAGATCAGGTTCATGCCGTTGTTGTAAAACTTGGTTTTGGCGGGTATGTTTTCGTGAGGAATGCTTTGATTCATTTGTACTGTTCTTGCTCTAGAATTGAATGCTCGAAACGGGTGTTTGAGGAGAATGTTAGTTCCCGAGATGTGGTTACTTGGAACTCAATGCTGACTGCTTTTGTAAGAGACGAGCAGATTGGTGTTGCAGAGAAGCTGTTTGAGGAAATGCCCGCGAGAGACGTGATATCGTGGAGCACGATGATCTCGGGTTACGTCCAAAATGGAGGGTTAGAACAAGGGTTGGAGTGCTTTAAACGGATGAGGGAGGAAGGGATTAGGATGAATGAGGCCACATTAGTCTCTGTTCTTTCAGCGTCCGCGCAATTGGGGTTGCTCGAACATGGTAGATTAGTTCACTCGCTTGCTGAGTCTTTGAATTTTCCTTTGACAGCTTGCCTCGGCACAGCTCTGATTGACATGTACGCAAAATGTGGATGCATCGAACAGTCTAAACTCTTGTTTAAAAACATGACCCAGAGAGATATTTGGACATGGAATGTCATGATCTGCGGATTGGCATCGCATGGCCTTGGGAAAGAAGCACTTGCACTTTTTGACAGGTTCGTTGATGAGGGTTTCCGTCCCGCAAATGTGACTTTCATTGGTGTCTTGGGTGCCTGTAGTAGAGCAGGTTTGGTACGCGAAGGAAGACATCATTTTAAGTTGATGACGGAAAAGTATGGCATATTACCTGAGATGGAGCATTATGGGTGCATGGTTGATATCTTGGGCCGTGCAGGGTTTGTAGATGAAGCTGTTGAGTTGATTGAGAAGATGACGGTTTCCCCAGATCCTGTATTATGGGCAACACTCCTTGGTGCCTGTAAGATACATGGATCAATAGAATTGGGTGAAAAGATTGGGAACAAATTACTAGAACTGGATCCAACCCATGACGGGCATTATGTACAACTAGCCAATATATATGCAAAGGCAAGGAAATGGGAAGACGTTGTGAGAGTTCGGAGATTAATGGTTGAGCAAAATACTAATAAAGCTGCAGGTTGGAGCTTGATTGAAGCACAGGGTAGAGTTCATAAATTTGTTGCAGGGGATAGAGAGCACGAACGCTCTCTAGAGATTCACAAAATGCTGGAGACAATTGGAACACGAATAGCAGAAGCTGGCTACTCACCGAATGTTACATCAGTTTTGCATGATATAGGGGAGGAAGAAAAAGAGAACGTTATAATGGAGCACAGCGAGCGACTTGCGATGGCTTTTGGCCTGTTAGTGACAGAGGCTGGGGAGTGCATTCGAATCATGAAGAATCTGAGAGTTTGTGAGGATTGTCATGAGGTAAGCAAGATAATTTCGAAGGTGTTTGAGAGAGAAATTATAGTGAGGGATGGAAGCAGATTTCACCATTTCAAGGACGGAAAATGTTCCTGCCTTGATTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

635

Amino Acids

70.88

Weight (kDa)

7.14

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 194 - 226 2.5e-06 PPR repeat family
PPR_2 PF13041 225 - 271 2.9e-08 PPR repeat family
PPR PF01535 228 - 258 8.1e-08 PPR repeat
PPR_2 PF13041 327 - 374 2.3e-06 PPR repeat family
PPR PF01535 330 - 359 3.1e-06 PPR repeat
E_motif PF20431 444 - 506 1.1e-16 E motif
DYW_deaminase PF14432 542 - 634 6.4e-43 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016920)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17040
malus_domestica MD15G1440600.v1.1
prunus_persica Prupe.1G582700_v2.0.a1 Prupe.1G582700_v2.0.a1
pyrus_communis pycom15g38840
rosa_chinensis RchiOBHm_Chr7g0220271
rosa_laevigata RLG00000002244
rosa_multiflora Rmu_sc0001136.1_g000028
rosa_roxburghii Rroxscaffold_3G00239660
rosa_rugosa Rorug07G0191700
rosa_samantha Rh7AG333700 Rh7BG323400 Rh7CG351300
rosa_wichuraiana Rw7G028150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1688
Acc36I ACCTGC 2 cut(s) 1118, 1490
AccB1I GGYRCC 2 cut(s) 1112, 1316
AccII CGCG 4 cut(s) 112, 671, 818, 1140
AciI CCGC 9 cut(s) 110, 124, 171, 301, 483, 669, 816, 1008, 1083
AclI AACGTT 1 cut(s) 1683
AclWI GGATC 6 cut(s) 292, 360, 1283, 1342, 1376, 1389
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 3 cut(s) 55, 874, 1538
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 4 cut(s) 521, 920, 1137, 1407
AfiI CCNNNNNNNGG 3 cut(s) 586, 1395, 1870
AflIII ACRYGT 1 cut(s) 915
AgsI TTSAA 6 cut(s) 54, 410, 542, 874, 1517, 1870
AjiI CACGTC 1 cut(s) 679
AjuI GAANNNNNNNTTGG 4 cut(s) 11, 34, 43, 66
AloI GAACNNNNNNTCC 2 cut(s) 435, 467
AluBI AGCT 8 cut(s) 413, 652, 890, 905, 1253, 1496, 1509, 1622
AluI AGCT 8 cut(s) 413, 652, 890, 905, 1253, 1496, 1509, 1622
Alw21I GWGCWC 3 cut(s) 695, 1563, 1698
Alw26I GTCTC 6 cut(s) 100, 163, 618, 669, 802, 1589
AlwI GGATC 6 cut(s) 292, 360, 1283, 1342, 1376, 1389
AlwNI CAGNNNCTG 3 cut(s) 1292, 1622, 1742
Ama87I CYCGRG 2 cut(s) 579, 703
AoxI GGCC 5 cut(s) 163, 786, 1024, 1228, 1723
ApeKI GCWGC 3 cut(s) 303, 417, 1496
ApoI RAATTY 3 cut(s) 55, 874, 1538
AseI ATTAAT 1 cut(s) 1469
Asp700I GAANNNNTTC 1 cut(s) 1882
AspLEI GCGC 3 cut(s) 114, 433, 820
AspS9I GGNCC 1 cut(s) 1228
AsuHPI GGTGA 3 cut(s) 1361, 1623, 1853
AsuII TTCGAA 2 cut(s) 1756, 1814
AvaI CYCGRG 2 cut(s) 579, 703
BamHI GGATCC 1 cut(s) 1381
BanI GGYRCC 2 cut(s) 1112, 1316
BbsI GAAGAC 2 cut(s) 1153, 1452
Bbv12I GWGCWC 3 cut(s) 695, 1563, 1698
BbvI GCAGC 3 cut(s) 290, 429, 1483
BccI CCATC 7 cut(s) 40, 269, 280, 365, 1189, 1708, 1841
BceAI ACGGC 2 cut(s) 443, 1215
BcgI CGANNNNNNTGC 2 cut(s) 1691, 1725
BcoDI GTCTC 6 cut(s) 100, 163, 618, 669, 802, 1589
BfaI CTAG 4 cut(s) 534, 1373, 1412, 1574
BfmI CTRYAG 2 cut(s) 1117, 1497
BfoI RGCGCY 1 cut(s) 434
BfuAI ACCTGC 2 cut(s) 1118, 1490
BisI GCNGC 4 cut(s) 171, 304, 418, 1497
BlsI GCNGC 4 cut(s) 172, 305, 419, 1498
BmeT110I CYCGRG 2 cut(s) 579, 703
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 1 cut(s) 1228
BmiI GGNNCC 3 cut(s) 1114, 1318, 1383
BmsI GCATC 3 cut(s) 923, 945, 1025
BpiI GAAGAC 2 cut(s) 1153, 1452
BpmI CTGGAG 1 cut(s) 1613
Bpu14I TTCGAA 2 cut(s) 1756, 1814
BsaBI GATNNNNATC 1 cut(s) 1015
BsaJI CCNNGG 3 cut(s) 895, 1027, 1312
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 435, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 586, 1395, 1870
Bse1I ACTGG 1 cut(s) 1383
Bse8I GATNNNNATC 1 cut(s) 1015
BseDI CCNNGG 3 cut(s) 895, 1027, 1312
BseGI GGATG 5 cut(s) 357, 762, 783, 938, 1852
BseJI GATNNNNATC 1 cut(s) 1015
BseLI CCNNNNNNNGG 3 cut(s) 586, 1395, 1870
BseMII CTCAG 3 cut(s) 855, 1182, 1763
BseNI ACTGG 1 cut(s) 1383
BseRI GAGGAG 7 cut(s) 64, 67, 70, 73, 76, 188, 578
BseXI GCAGC 3 cut(s) 290, 429, 1483
BseYI CCCAGC 1 cut(s) 1742
BsgI GTGCAG 2 cut(s) 84, 1254
Bsh1236I CGCG 4 cut(s) 112, 671, 818, 1140
BshFI GGCC 5 cut(s) 165, 788, 1026, 1230, 1725
BshNI GGYRCC 2 cut(s) 1112, 1316
BsiHKAI GWGCWC 3 cut(s) 695, 1563, 1698
BsiHKCI CYCGRG 2 cut(s) 579, 703
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 3 cut(s) 586, 1395, 1870
BsmAI GTCTC 6 cut(s) 100, 163, 618, 669, 802, 1589
BsmBI CGTCTC 3 cut(s) 163, 618, 669
BsmFI GGGAC 1 cut(s) 1065
BsmI GAATGC 3 cut(s) 508, 548, 1752
BsnI GGCC 5 cut(s) 165, 788, 1026, 1230, 1725
BsoBI CYCGRG 2 cut(s) 579, 703
Bsp119I TTCGAA 2 cut(s) 1756, 1814
Bsp1286I GDGCHC 3 cut(s) 695, 1563, 1698
Bsp1407I TGTACA 1 cut(s) 1405
Bsp143I GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
BspACI CCGC 9 cut(s) 110, 124, 171, 301, 483, 669, 816, 1008, 1083
BspANI GGCC 5 cut(s) 165, 788, 1026, 1230, 1725
BspCNI CTCAG 3 cut(s) 856, 1183, 1764
BspFNI CGCG 4 cut(s) 112, 671, 818, 1140
BspHI TCATGA 3 cut(s) 999, 1761, 1792
BspLI GGNNCC 3 cut(s) 1114, 1318, 1383
BspMAI CTGCAG 1 cut(s) 1501
BspMI ACCTGC 2 cut(s) 1118, 1490
BspPI GGATC 6 cut(s) 292, 360, 1283, 1342, 1376, 1389
BspT104I TTCGAA 2 cut(s) 1756, 1814
BspT107I GGYRCC 2 cut(s) 1112, 1316
BsrGI TGTACA 1 cut(s) 1405
BsrI ACTGG 1 cut(s) 1383
BssECI CCNNGG 3 cut(s) 895, 1027, 1312
BssMI GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
BssT1I CCWWGG 2 cut(s) 1027, 1312
Bst4CI ACNGT 3 cut(s) 524, 945, 1279
Bst6I CTCTTC 2 cut(s) 32, 42
BstAUI TGTACA 1 cut(s) 1405
BstBI TTCGAA 2 cut(s) 1756, 1814
BstC8I GCNNGC 7 cut(s) 138, 415, 669, 861, 892, 1624, 1703
BstDEI CTNAG 3 cut(s) 864, 1191, 1772
BstF5I GGATG 5 cut(s) 357, 762, 783, 938, 1852
BstFNI CGCG 4 cut(s) 112, 671, 818, 1140
BstH2I RGCGCY 1 cut(s) 434
BstHHI GCGC 3 cut(s) 114, 433, 820
BstKTI GATC 8 cut(s) 300, 368, 448, 702, 1005, 1291, 1337, 1384
BstMAI GTCTC 6 cut(s) 100, 163, 618, 669, 802, 1589
BstMBI GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
BstMWI GCNNNNNNNGC 1 cut(s) 1702
BstNSI RCATGY 1 cut(s) 919
BstSFI CTRYAG 2 cut(s) 1117, 1497
BstUI CGCG 4 cut(s) 112, 671, 818, 1140
BstV1I GCAGC 3 cut(s) 290, 429, 1483
BstV2I GAAGAC 2 cut(s) 1153, 1452
BstX2I RGATCY 2 cut(s) 1288, 1381
BstYI RGATCY 2 cut(s) 1288, 1381
BsuRI GGCC 5 cut(s) 165, 788, 1026, 1230, 1725
BtgZI GCGATG 2 cut(s) 1002, 1727
BtrI CACGTC 1 cut(s) 679
BtsCI GGATG 5 cut(s) 357, 762, 783, 938, 1852
BtsIMutI CAGTG 1 cut(s) 435
BveI ACCTGC 2 cut(s) 1118, 1490
Cac8I GCNNGC 7 cut(s) 138, 415, 669, 861, 892, 1624, 1703
CaiI CAGNNNCTG 3 cut(s) 1292, 1622, 1742
CciI TCATGA 3 cut(s) 999, 1761, 1792
CfoI GCGC 3 cut(s) 114, 433, 820
Cfr13I GGNCC 1 cut(s) 1228
CseI GACGC 2 cut(s) 361, 801
Csp6I GTAC 4 cut(s) 520, 919, 1136, 1406
CviQI GTAC 4 cut(s) 520, 919, 1136, 1406
DdeI CTNAG 3 cut(s) 864, 1191, 1772
DpnI GATC 8 cut(s) 299, 367, 447, 701, 1004, 1290, 1336, 1383
DpnII GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
DraI TTTAAA 2 cut(s) 751, 961
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 32, 42
EarI CTCTTC 2 cut(s) 32, 42
EciI GGCGGA 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 1027, 1312
Eco32I GATATC 2 cut(s) 684, 1222
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 579, 703
EcoRV GATATC 2 cut(s) 684, 1222
EcoT14I CCWWGG 2 cut(s) 1027, 1312
EcoT22I ATGCAT 1 cut(s) 938
ErhI CCWWGG 2 cut(s) 1027, 1312
Esp3I CGTCTC 3 cut(s) 163, 618, 669
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FaqI GGGAC 1 cut(s) 1065
FauI CCCGC 3 cut(s) 476, 676, 1090
Fnu4HI GCNGC 4 cut(s) 171, 304, 418, 1497
FokI GGATG 5 cut(s) 344, 769, 790, 945, 1859
Fsp4HI GCNGC 4 cut(s) 171, 304, 418, 1497
FspBI CTAG 4 cut(s) 534, 1373, 1412, 1574
GlaI GCGC 3 cut(s) 113, 432, 819
GluI GCNGC 4 cut(s) 171, 304, 418, 1497
GsaI CCCAGC 1 cut(s) 1746
GsuI CTGGAG 1 cut(s) 1613
HaeII RGCGCY 1 cut(s) 434
HaeIII GGCC 5 cut(s) 165, 788, 1026, 1230, 1725
HapII CCGG 1 cut(s) 162
HgaI GACGC 2 cut(s) 361, 801
HhaI GCGC 3 cut(s) 114, 433, 820
Hin6I GCGC 3 cut(s) 112, 431, 818
HinP1I GCGC 3 cut(s) 112, 431, 818
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 6 cut(s) 320, 511, 866, 1579, 1758, 1768
HpaII CCGG 1 cut(s) 162
HphI GGTGA 3 cut(s) 1361, 1623, 1853
Hpy166II GTNNAC 1 cut(s) 853
Hpy188I TCNGA 3 cut(s) 909, 1464, 1773
Hpy188III TCNNGA 7 cut(s) 496, 534, 579, 1000, 1574, 1762, 1793
Hpy8I GTNNAC 1 cut(s) 853
HpyAV CCTTC 5 cut(s) 126, 434, 761, 1136, 1810
HpyCH4III ACNGT 3 cut(s) 524, 945, 1279
HpyCH4IV ACGT 4 cut(s) 678, 711, 1449, 1683
HpyF10VI GCNNNNNNNGC 1 cut(s) 1702
HpyF3I CTNAG 3 cut(s) 864, 1191, 1772
HpySE526I ACGT 4 cut(s) 678, 711, 1449, 1683
HspAI GCGC 3 cut(s) 112, 431, 818
Kzo9I GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
LmnI GCTCC 5 cut(s) 359, 690, 1198, 1506, 1693
Lsp1109I GCAGC 3 cut(s) 290, 429, 1483
LweI GCATC 3 cut(s) 923, 945, 1025
MaeI CTAG 4 cut(s) 534, 1373, 1412, 1574
MaeII ACGT 4 cut(s) 678, 711, 1449, 1683
MaeIII GTNAC 5 cut(s) 589, 707, 1090, 1639, 1732
MalI GATC 8 cut(s) 299, 367, 447, 701, 1004, 1290, 1336, 1383
MboI GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
MboII GAAGA 7 cut(s) 19, 29, 1158, 1282, 1457, 1682, 1777
MfeI CAATTG 2 cut(s) 821, 1599
MflI RGATCY 2 cut(s) 1288, 1381
MhlI GDGCHC 3 cut(s) 695, 1563, 1698
MlyI GAGTC 1 cut(s) 875
MmeI TCCRAC 3 cut(s) 720, 1409, 1484
Mph1103I ATGCAT 1 cut(s) 938
MroXI GAANNNNTTC 1 cut(s) 1882
MseI TTAA 4 cut(s) 750, 960, 1158, 1469
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 1 cut(s) 162
MunI CAATTG 2 cut(s) 821, 1599
Mva1269I GAATGC 3 cut(s) 508, 548, 1752
MvnI CGCG 4 cut(s) 112, 671, 818, 1140
MwoI GCNNNNNNNGC 1 cut(s) 1702
NdeII GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
NlaIV GGNNCC 3 cut(s) 1114, 1318, 1383
NmeAIII GCCGAG 1 cut(s) 876
NmuCI GTSAC 2 cut(s) 1090, 1732
NsiI ATGCAT 1 cut(s) 938
NspI RCATGY 1 cut(s) 919
NspV TTCGAA 2 cut(s) 1756, 1814
PagI TCATGA 3 cut(s) 999, 1761, 1792
PciI ACATGT 1 cut(s) 915
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 692
PctI GAATGC 3 cut(s) 508, 548, 1752
PdmI GAANNNNTTC 1 cut(s) 1882
PfeI GAWTC 5 cut(s) 320, 511, 1579, 1758, 1768
PkrI GCNGC 4 cut(s) 172, 305, 419, 1498
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
PscI ACATGT 1 cut(s) 915
PshBI ATTAAT 1 cut(s) 1469
PsiI TTATAA 1 cut(s) 1688
Psp1406I AACGTT 1 cut(s) 1683
PspFI CCCAGC 1 cut(s) 1742
PspN4I GGNNCC 3 cut(s) 1114, 1318, 1383
PspPI GGNCC 1 cut(s) 1228
PstI CTGCAG 1 cut(s) 1501
PstNI CAGNNNCTG 3 cut(s) 1292, 1622, 1742
PsuI RGATCY 2 cut(s) 1288, 1381
RsaI GTAC 4 cut(s) 521, 920, 1137, 1407
RsaNI GTAC 4 cut(s) 520, 919, 1136, 1406
SaqAI TTAA 4 cut(s) 750, 960, 1158, 1469
SatI GCNGC 4 cut(s) 171, 304, 418, 1497
Sau3AI GATC 8 cut(s) 297, 365, 445, 699, 1002, 1288, 1334, 1381
Sau96I GGNCC 1 cut(s) 1228
SchI GAGTC 1 cut(s) 875
SduI GDGCHC 3 cut(s) 695, 1563, 1698
SfaNI GCATC 3 cut(s) 923, 945, 1025
SfcI CTRYAG 2 cut(s) 1117, 1497
SfuI TTCGAA 2 cut(s) 1756, 1814
SsiI CCGC 9 cut(s) 110, 124, 171, 301, 483, 669, 816, 1008, 1083
SspMI CTAG 4 cut(s) 534, 1373, 1412, 1574
StyI CCWWGG 2 cut(s) 1027, 1312
TaaI ACNGT 3 cut(s) 524, 945, 1279
TaiI ACGT 4 cut(s) 681, 714, 1452, 1686
TaqI TCGA 5 cut(s) 548, 834, 939, 1756, 1814
TatI WGTACW 2 cut(s) 519, 1405
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 5 cut(s) 320, 511, 1579, 1758, 1768
Tru1I TTAA 4 cut(s) 750, 960, 1158, 1469
Tru9I TTAA 4 cut(s) 750, 960, 1158, 1469
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 2 cut(s) 1090, 1732
TseI GCWGC 3 cut(s) 303, 417, 1496
Tsp45I GTSAC 2 cut(s) 1090, 1732
TspDTI ATGAA 7 cut(s) 443, 503, 794, 1087, 1263, 1523, 1778
TspGWI ACGGA 4 cut(s) 769, 1067, 1184, 1890
TspRI CASTG 1 cut(s) 442
VspI ATTAAT 1 cut(s) 1469
XapI RAATTY 3 cut(s) 55, 874, 1538
XbaI TCTAGA 2 cut(s) 533, 1573
XceI RCATGY 1 cut(s) 919
XmnI GAANNNNTTC 1 cut(s) 1882
XspI CTAG 4 cut(s) 534, 1373, 1412, 1574
Zsp2I ATGCAT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.