Rmu_sc0001136.1_g000028

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001136.1
OG0016920
Physical Location & Seq
Reverse (-)
133113 .. 134987
1875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001136.1_g000028.1.cds

Sequence Viewer

Length: 1875 bp
atggtggttgcttataaaacccaaaaagcagggaccaaatccctcctcctgttttctcttttttcccaacattcaaaacctgtttcggtgaagcaaaccaagcaagcccatgctcgtatcatcgtctccggcctcaccgccaacacccaattcatggctcacctcctctgctcccttgctctctcctcatcaaccccatttcactattctctctctctttaccactccatcacacaccccactgttttcgcctccaataacatgatccgctgcttctccaaaagccactcaccccgacactccctgcttctctactcttcaatgctgcgaagaagctgctattcccaacccaacaaccacactttcactttcttgttgcaagcttgcagcgaggcctccgcgctctgcgaaggagctcaggtccatgttcaagcactcaaacatggtttcgctgacttcgtgtttgtcaggaacgctttgattcatttctattgttcttgttctagtactgaatgctccaagagggtgtttgacgacaatgcgcactcccgagatatagttacttggaactcaatgctcgctgcttttgtcagaggcggacagattgataatgcacgcaaggtgtttgatgaaatgccccagagagatgtgatctcatggagcactgttatctccggttacgtgcaaaacgggcagttcgaggaagggttggagtgctttaaagagatgagagacaagggcatgaggttgaatgaggccacattagtctcggtgctttcggcatccgcccagatgggtttgctagaacacggtaggttagttcactcgcttgtagagtcgttgaaactcccgctgactgtttcgcttggcacagcactaatcgacatgtatgccaaatgtggatgcattgaacagtctaaacacttgtttgacaatatgcccctgagagatatatggacatggaatgttatgatctgtggattggcctctcacggccttgcaaaagaggcgctcgaacactttgagaggttaactaacgacggtctctgtcccgccaatgtgacgttcattggtgtcttgggtgcctgcagtagagcaggtttggttagtgaaggacgacattattttaaattaatgaccgacaagtacggcattcagccagagatggagcattatgggtgcatggttgatctcttaggccgtgcaggctttgtagatgaagctgctgagatgattgacaagatgacagttccaccagatcctgtattatgggcaacgctacttggtgcttgcaagatgcatggctcaatagaattgggagaaaaaattgggaataagttgattcaattggatccaacccatgatgggcattatgtacaactagccagtatatatgcaaaagcaaagaaatgggaagatgttgctagagttaggagattgatggctgcgaaaaacaccaataaagctgcaggctggagtttgattgaagcgcaaggtaaagttcataaatttgttgcaggggatagagagcatgaacgttctcatgagatttataaaatgctagagacaattgtaacacgaatagcagaagctggctactcgccaaatgtttcatcggtgttgcatgatataggggaggaagaaaaggagaatgcaataaaggagcatagtgagcggcttgcaatggcatttggcttgttggtaacaggggatggggattgcattcggattgtgaagaatttgagagtttgtgaggactgccatgaggtaagcaagataatatcaaaggtgtttgggagagaaattatagtgagagatggaagcagatttcatcatttcaaggagggcaagtgttcttgccttgattattggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

624

Amino Acids

69.69

Weight (kDa)

7.81

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016920)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17040
malus_domestica MD15G1440600.v1.1
prunus_persica Prupe.1G582700_v2.0.a1 Prupe.1G582700_v2.0.a1
pyrus_communis pycom15g38840
rosa_chinensis RchiOBHm_Chr7g0220271
rosa_laevigata RLG00000002244
rosa_multiflora Rmu_sc0001136.1_g000028
rosa_roxburghii Rroxscaffold_3G00239660
rosa_rugosa Rorug07G0191700
rosa_samantha Rh7AG333700 Rh7BG323400 Rh7CG351300
rosa_wichuraiana Rw7G028150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 15, 1554
Acc16I TGCGCA 1 cut(s) 543
Acc36I ACCTGC 1 cut(s) 1088
AccB1I GGYRCC 1 cut(s) 1082
AccB7I CCANNNNNTGG 1 cut(s) 154
AccBSI CCGCTC 1 cut(s) 1675
AccII CGCG 1 cut(s) 401
AciI CCGC 8 cut(s) 138, 268, 399, 597, 786, 851, 1053, 1675
AclI AACGTT 1 cut(s) 1537
AclWI GGATC 4 cut(s) 259, 1253, 1346, 1359
AcsI RAATTY 2 cut(s) 1508, 1738
AfaI GTAC 3 cut(s) 508, 1148, 1377
AfiI CCNNNNNNNGG 2 cut(s) 154, 1365
AflIII ACRYGT 1 cut(s) 885
AgsI TTSAA 9 cut(s) 75, 321, 431, 751, 844, 911, 1346, 1487, 1840
AjuI GAANNNNNNNTTGG 4 cut(s) 134, 166, 1049, 1081
AluBI AGCT 6 cut(s) 336, 383, 416, 1223, 1466, 1592
AluI AGCT 6 cut(s) 336, 383, 416, 1223, 1466, 1592
Alw21I GWGCWC 2 cut(s) 418, 665
Alw26I GTCTC 5 cut(s) 130, 726, 772, 1049, 1559
AlwI GGATC 4 cut(s) 259, 1253, 1346, 1359
AlwNI CAGNNNCTG 2 cut(s) 1262, 1592
Ama87I CYCGRG 1 cut(s) 549
AoxI GGCC 6 cut(s) 130, 393, 756, 984, 994, 1198
ApeKI GCWGC 8 cut(s) 270, 325, 336, 387, 581, 1223, 1445, 1466
ApoI RAATTY 2 cut(s) 1508, 1738
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AseI ATTAAT 1 cut(s) 1133
AspLEI GCGC 4 cut(s) 403, 544, 1012, 1492
AspS9I GGNCC 2 cut(s) 33, 421
AsuHPI GGTGA 4 cut(s) 100, 127, 152, 282
AvaI CYCGRG 1 cut(s) 549
AvaII GGWCC 2 cut(s) 33, 421
BamHI GGATCC 1 cut(s) 1351
BanI GGYRCC 1 cut(s) 1082
BanII GRGCYC 1 cut(s) 418
Bbv12I GWGCWC 2 cut(s) 418, 665
BbvI GCAGC 8 cut(s) 257, 312, 323, 399, 568, 1210, 1432, 1453
BccI CCATC 7 cut(s) 236, 787, 1159, 1358, 1435, 1706, 1811
BceAI ACGGC 3 cut(s) 1009, 1165, 1185
BcgI CGANNNNNNTGC 4 cut(s) 318, 352, 872, 906
BcoDI GTCTC 5 cut(s) 130, 726, 772, 1049, 1559
BfaI CTAG 5 cut(s) 504, 803, 1382, 1425, 1562
BfmI CTRYAG 2 cut(s) 1087, 1467
BfoI RGCGCY 1 cut(s) 1013
BfuAI ACCTGC 1 cut(s) 1088
BglI GCCNNNNNGGC 1 cut(s) 1206
BisI GCNGC 9 cut(s) 271, 326, 337, 388, 582, 1224, 1446, 1467, 1676
BlsI GCNGC 9 cut(s) 272, 327, 338, 389, 583, 1225, 1447, 1468, 1677
BmcAI AGTACT 1 cut(s) 508
Bme18I GGWCC 2 cut(s) 33, 421
BmeT110I CYCGRG 1 cut(s) 549
BmgT120I GGNCC 2 cut(s) 33, 421
BmiI GGNNCC 3 cut(s) 34, 1084, 1353
BmsI GCATC 3 cut(s) 791, 893, 1287
BpmI CTGGAG 1 cut(s) 1495
Bpu10I CCTNAGC 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 682
BsaI GGTCTC 1 cut(s) 1049
BsaWI WCCGGW 1 cut(s) 674
BsaXI ACNNNNNCTCC 4 cut(s) 405, 435, 1835, 1865
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 1365
Bse1I ACTGG 1 cut(s) 1386
Bse3DI GCAATG 1 cut(s) 1689
BseGI GGATG 3 cut(s) 782, 908, 1717
BseLI CCNNNNNNNGG 2 cut(s) 154, 1365
BseMI GCAATG 1 cut(s) 1689
BseMII CTCAG 3 cut(s) 431, 935, 1218
BseNI ACTGG 1 cut(s) 1386
BseRI GAGGAG 3 cut(s) 35, 155, 175
BseXI GCAGC 8 cut(s) 257, 312, 323, 399, 568, 1210, 1432, 1453
BsgI GTGCAG 1 cut(s) 1224
Bsh1236I CGCG 1 cut(s) 401
BshFI GGCC 6 cut(s) 132, 395, 758, 986, 996, 1200
BshNI GGYRCC 1 cut(s) 1082
BsiHKAI GWGCWC 2 cut(s) 418, 665
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 2 cut(s) 129, 675
BslFI GGGAC 2 cut(s) 46, 1035
BslI CCNNNNNNNGG 2 cut(s) 154, 1365
BsmAI GTCTC 5 cut(s) 130, 726, 772, 1049, 1559
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 2 cut(s) 46, 1035
BsmI GAATGC 4 cut(s) 518, 1152, 1657, 1722
BsnI GGCC 6 cut(s) 132, 395, 758, 986, 996, 1200
Bso31I GGTCTC 1 cut(s) 1049
BsoBI CYCGRG 1 cut(s) 549
Bsp1286I GDGCHC 2 cut(s) 418, 665
Bsp1407I TGTACA 1 cut(s) 1375
Bsp143I GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
BspACI CCGC 8 cut(s) 138, 268, 399, 597, 786, 851, 1053, 1675
BspANI GGCC 6 cut(s) 132, 395, 758, 986, 996, 1200
BspCNI CTCAG 3 cut(s) 430, 936, 1219
BspFNI CGCG 1 cut(s) 401
BspHI TCATGA 1 cut(s) 1543
BspLI GGNNCC 3 cut(s) 34, 1084, 1353
BspMAI CTGCAG 2 cut(s) 1091, 1471
BspMI ACCTGC 1 cut(s) 1088
BspPI GGATC 4 cut(s) 259, 1253, 1346, 1359
BspT107I GGYRCC 1 cut(s) 1082
BspTNI GGTCTC 1 cut(s) 1049
BsrBI CCGCTC 1 cut(s) 1675
BsrDI GCAATG 1 cut(s) 1689
BsrGI TGTACA 1 cut(s) 1375
BsrI ACTGG 1 cut(s) 1386
BssMI GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
Bst4CI ACNGT 7 cut(s) 244, 667, 812, 859, 915, 1043, 1249
Bst6I CTCTTC 1 cut(s) 322
BstAUI TGTACA 1 cut(s) 1375
BstBAI YACGTR 1 cut(s) 682
BstDEI CTNAG 4 cut(s) 417, 944, 1195, 1227
BstF5I GGATG 3 cut(s) 782, 908, 1717
BstFNI CGCG 1 cut(s) 401
BstH2I RGCGCY 1 cut(s) 1013
BstHHI GCGC 4 cut(s) 403, 544, 1012, 1492
BstKTI GATC 6 cut(s) 267, 654, 975, 1192, 1261, 1354
BstMAI GTCTC 5 cut(s) 130, 726, 772, 1049, 1559
BstMBI GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
BstMWI GCNNNNNNNGC 5 cut(s) 100, 691, 1007, 1206, 1672
BstNSI RCATGY 1 cut(s) 889
BstSFI CTRYAG 2 cut(s) 1087, 1467
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 8 cut(s) 257, 312, 323, 399, 568, 1210, 1432, 1453
BstX2I RGATCY 2 cut(s) 1258, 1351
BstYI RGATCY 2 cut(s) 1258, 1351
BsuRI GGCC 6 cut(s) 132, 395, 758, 986, 996, 1200
BtsCI GGATG 3 cut(s) 782, 908, 1717
BtsIMutI CAGTG 2 cut(s) 240, 663
BveI ACCTGC 1 cut(s) 1088
CaiI CAGNNNCTG 2 cut(s) 1262, 1592
CciI TCATGA 1 cut(s) 1543
CfoI GCGC 4 cut(s) 403, 544, 1012, 1492
Cfr13I GGNCC 2 cut(s) 33, 421
Csp6I GTAC 3 cut(s) 507, 1147, 1376
CviQI GTAC 3 cut(s) 507, 1147, 1376
DdeI CTNAG 4 cut(s) 417, 944, 1195, 1227
DpnI GATC 6 cut(s) 266, 653, 974, 1191, 1260, 1353
DpnII GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
DraI TTTAAA 2 cut(s) 721, 1129
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
EciI GGCGGA 2 cut(s) 612, 775
Ecl136II GAGCTC 1 cut(s) 416
Eco147I AGGCCT 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 1049
Eco47I GGWCC 2 cut(s) 33, 421
Eco53kI GAGCTC 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 549
EcoICRI GAGCTC 1 cut(s) 416
EcoT22I ATGCAT 2 cut(s) 908, 1302
EcoT38I GRGCYC 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 130
FaqI GGGAC 2 cut(s) 46, 1035
FauI CCCGC 2 cut(s) 858, 1060
Fnu4HI GCNGC 9 cut(s) 271, 326, 337, 388, 582, 1224, 1446, 1467, 1676
FokI GGATG 3 cut(s) 769, 915, 1724
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 9 cut(s) 271, 326, 337, 388, 582, 1224, 1446, 1467, 1676
FspAI RTGCGCAY 1 cut(s) 543
FspBI CTAG 5 cut(s) 504, 803, 1382, 1425, 1562
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 4 cut(s) 402, 543, 1011, 1491
GluI GCNGC 9 cut(s) 271, 326, 337, 388, 582, 1224, 1446, 1467, 1676
GsuI CTGGAG 1 cut(s) 1495
HaeII RGCGCY 1 cut(s) 1013
HaeIII GGCC 6 cut(s) 132, 395, 758, 986, 996, 1200
HapII CCGG 2 cut(s) 129, 675
HhaI GCGC 4 cut(s) 403, 544, 1012, 1492
Hin6I GCGC 4 cut(s) 401, 542, 1010, 1490
HinP1I GCGC 4 cut(s) 401, 542, 1010, 1490
HincII GTYRAC 1 cut(s) 1032
HindII GTYRAC 1 cut(s) 1032
HindIII AAGCTT 1 cut(s) 381
HinfI GANTC 3 cut(s) 481, 836, 1342
HpaI GTTAAC 1 cut(s) 1032
HpaII CCGG 2 cut(s) 129, 675
HphI GGTGA 4 cut(s) 100, 127, 152, 282
Hpy166II GTNNAC 2 cut(s) 823, 1032
Hpy188I TCNGA 2 cut(s) 593, 1728
Hpy188III TCNNGA 3 cut(s) 469, 549, 1544
Hpy8I GTNNAC 2 cut(s) 823, 1032
Hpy99I CGWCG 1 cut(s) 1043
HpyAV CCTTC 3 cut(s) 404, 698, 1106
HpyCH4III ACNGT 7 cut(s) 244, 667, 812, 859, 915, 1043, 1249
HpyCH4IV ACGT 3 cut(s) 681, 1064, 1537
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 691, 1007, 1206, 1672
HpyF3I CTNAG 4 cut(s) 417, 944, 1195, 1227
HpySE526I ACGT 3 cut(s) 681, 1064, 1537
HspAI GCGC 4 cut(s) 401, 542, 1010, 1490
KspAI GTTAAC 1 cut(s) 1032
Kzo9I GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
LmnI GCTCC 6 cut(s) 176, 413, 521, 660, 1168, 1663
Lsp1109I GCAGC 8 cut(s) 257, 312, 323, 399, 568, 1210, 1432, 1453
LweI GCATC 3 cut(s) 791, 893, 1287
MaeI CTAG 5 cut(s) 504, 803, 1382, 1425, 1562
MaeII ACGT 3 cut(s) 681, 1064, 1537
MaeIII GTNAC 5 cut(s) 559, 677, 1060, 1573, 1702
MalI GATC 6 cut(s) 266, 653, 974, 1191, 1260, 1353
MbiI CCGCTC 1 cut(s) 1675
MboI GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
MboII GAAGA 5 cut(s) 309, 342, 1427, 1652, 1747
MfeI CAATTG 2 cut(s) 1346, 1569
MflI RGATCY 2 cut(s) 1258, 1351
MhlI GDGCHC 2 cut(s) 418, 665
MluCI AATT 9 cut(s) 149, 1130, 1313, 1326, 1346, 1508, 1569, 1738, 1803
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 2 cut(s) 690, 1379
Mph1103I ATGCAT 2 cut(s) 908, 1302
MseI TTAA 4 cut(s) 720, 1031, 1128, 1133
MspA1I CMGCKG 2 cut(s) 270, 853
MspI CCGG 2 cut(s) 129, 675
MunI CAATTG 2 cut(s) 1346, 1569
Mva1269I GAATGC 4 cut(s) 518, 1152, 1657, 1722
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 5 cut(s) 100, 691, 1007, 1206, 1672
NdeII GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
NlaIV GGNNCC 3 cut(s) 34, 1084, 1353
NmuCI GTSAC 1 cut(s) 1060
NsbI TGCGCA 1 cut(s) 543
NsiI ATGCAT 2 cut(s) 908, 1302
NspI RCATGY 1 cut(s) 889
PagI TCATGA 1 cut(s) 1543
PceI AGGCCT 1 cut(s) 395
PciI ACATGT 1 cut(s) 885
PcsI WCGNNNNNNNCGW 2 cut(s) 456, 696
PctI GAATGC 4 cut(s) 518, 1152, 1657, 1722
PfeI GAWTC 2 cut(s) 481, 1342
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 9 cut(s) 272, 327, 338, 389, 583, 1225, 1447, 1468, 1677
PleI GAGTC 1 cut(s) 844
PpsI GAGTC 1 cut(s) 844
Ppu21I YACGTR 1 cut(s) 682
PscI ACATGT 1 cut(s) 885
PshBI ATTAAT 1 cut(s) 1133
PsiI TTATAA 2 cut(s) 15, 1554
Psp124BI GAGCTC 1 cut(s) 418
Psp1406I AACGTT 1 cut(s) 1537
PspN4I GGNNCC 3 cut(s) 34, 1084, 1353
PspPI GGNCC 2 cut(s) 33, 421
PstI CTGCAG 2 cut(s) 1091, 1471
PstNI CAGNNNCTG 2 cut(s) 1262, 1592
PsuI RGATCY 2 cut(s) 1258, 1351
RsaI GTAC 3 cut(s) 508, 1148, 1377
RsaNI GTAC 3 cut(s) 507, 1147, 1376
SacI GAGCTC 1 cut(s) 418
SaqAI TTAA 4 cut(s) 720, 1031, 1128, 1133
SatI GCNGC 9 cut(s) 271, 326, 337, 388, 582, 1224, 1446, 1467, 1676
Sau3AI GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
Sau96I GGNCC 2 cut(s) 33, 421
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 1 cut(s) 845
SduI GDGCHC 2 cut(s) 418, 665
SfaNI GCATC 3 cut(s) 791, 893, 1287
SfcI CTRYAG 2 cut(s) 1087, 1467
SinI GGWCC 2 cut(s) 33, 421
Sse9I AATT 9 cut(s) 149, 1130, 1313, 1326, 1346, 1508, 1569, 1738, 1803
SseBI AGGCCT 1 cut(s) 395
SsiI CCGC 8 cut(s) 138, 268, 399, 597, 786, 851, 1053, 1675
SspMI CTAG 5 cut(s) 504, 803, 1382, 1425, 1562
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 1 cut(s) 395
TaaI ACNGT 7 cut(s) 244, 667, 812, 859, 915, 1043, 1249
TaiI ACGT 3 cut(s) 684, 1067, 1540
TaqI TCGA 3 cut(s) 699, 882, 1014
TaqII GACCGA 1 cut(s) 1154
TasI AATT 9 cut(s) 149, 1130, 1313, 1326, 1346, 1508, 1569, 1738, 1803
TatI WGTACW 2 cut(s) 506, 1375
TauI GCSGC 1 cut(s) 1678
TfiI GAWTC 2 cut(s) 481, 1342
Tru1I TTAA 4 cut(s) 720, 1031, 1128, 1133
Tru9I TTAA 4 cut(s) 720, 1031, 1128, 1133
TscAI CASTG 2 cut(s) 247, 670
TseFI GTSAC 1 cut(s) 1060
TseI GCWGC 8 cut(s) 270, 325, 336, 387, 581, 1223, 1445, 1466
Tsp45I GTSAC 1 cut(s) 1060
TspDTI ATGAA 9 cut(s) 142, 473, 645, 1057, 1233, 1493, 1548, 1602, 1820
TspRI CASTG 2 cut(s) 247, 670
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 33, 421
VspI ATTAAT 1 cut(s) 1133
XapI RAATTY 2 cut(s) 1508, 1738
XceI RCATGY 1 cut(s) 889
XspI CTAG 5 cut(s) 504, 803, 1382, 1425, 1562
ZrmI AGTACT 1 cut(s) 508
Zsp2I ATGCAT 2 cut(s) 908, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.