Rh7BG323400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0016920
Physical Location & Seq
Forward (+)
31734198 .. 31736818
2621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG323400.1

Sequence Viewer

Length: 1872 bp
ATGGTGGTTGCTTATAAACCCCAAAAAGCAGGGGCCAAATCCCTCCTGTTTTCTCTTTTTTCCCGACATTCAAAACCTGTTTCGGTGAAGCAAACCAAGCAAGCCCATGCTCGTATCATCGTCTCCGGCCTCACCGCCAACACCCAACTCATGGCTCACCTCCTCTGCTCCCTTGCTCTTTCCTCATCAACCCCATTTCACTATTCTCTCTCTCTTTACCACTCCATCACACACCCCACTGTTTTCGCCTCCAATAACATGATCCGCTGCTTCTCCAAAAGCCACTCACCTCGACACTCCCTGCTTCTCTACTCTTCAATGCTGCGAAGAAGCTGCTATTCCCAACCCAACAACCACACTTTCACTTTCTTGTTGCAAGCTTGCAGCGAGGCCTCCGCGCTCTGTGAAGGAGCTCAGGTCCATGCTCATGCACTCAAACATGGTTTCGCTGACTTCGTGTTTGTCACGAATGCTTTGATTCATTTCTATTGTTCTTGTTCTTGTATTGAATGCTCCATGAGGGTGTTTGACGACAATGCGCACTCCCGAGATATAGTTACTTGGAACTCAATGCTCGCTGCTCTTGTCAGAGGCGGACAGATTGATAACGCACGCAAGGTGTTTGATGAAATGCCCCAGAGAGATGTGATCTCATGGAGCACTGTTATCTCCGGTTACGTGCAAAACGGGCAGTTAGAGGAAGGGTTGGAGTGCTTTAAAGAGATGAGAGACAAGGGGATGAGGTTGAATGAGGCCACATTAGTCTCGGTGCTTTCGGCATCCGCCCAGATGGGTTTGCTAGAACACGGTAGGTTAGTTCACTCGCTTGTAGAGTCGTTGAAACTCCCGCTGACTGTTTCGCTTGGCACAGCACTAATCGACATGTATGCCAAATGTGGATGCATTGAACAGTCTAAACACTTGTTTAACAATATGCCCCTGAGAGATATATGGACATGGAATGTTATGATCTGTGGATTGGCCTCTCACGGCCTTGCAAAAGAGGCGCTCGAACATTTTGAGAGGTTAACTAACGACGGTCTCTGTCCCGCCAATGTGACGTTCATTGGTGTCTTGGGTGCCTGCAGTAGAGCAGGTTTGGTTAGTGAAGGACGACATTATTTTAAATTAATGACCGACAGGTACGGCATTCAGCCAGAGATGGAGCATTATGGGTGCATGGTTGATCTCTTAGGCCGTGCAGGCTTTGTCGATGAAGCCGCTGAGATGATTGACAAGATGACGGTTCCACCAGATCCTGTATTATGGGCAACGCTACTTGGTGCTTGCAAGATGCATGGCTCAATAGAATTGGGAGAAAAGATTGGGAATAAGTTGATTCAATTGGATCCAACCCATGATGGGCATTATGTACAACTAGCCAGTATATATGCAAAAGCAAAGAAATGGGAAGATGTTGTTAGAGTTCGGAGATTGATGGCTGCAAAAAACACCAATAAAGCTGCAGGTTGGAGTTTGATTGAAGCACAAGGTAAAGTTCATAGATTTGTTGCAGGGGATAGAGAGCATGAACGTTCTCGAGAGATTTATAAAATGCTAGAGACTATTGTAACACGAATAGCGGAAGCTGGCTACTCGCCAAATGTTTTGTCGGTGTTGCATGATATAGGGGAGGAAGAAAAGGAGAATGCAATAAAGGAGCATAGTGAGCGGCTTGCAATGGCATTTGGCTTGTTGGTAACAGGGGATGGTGATTGCATTCGGATTGTGAAGAATTTGAGAGTTTGTGAGGACTGCCATGAGGTAAGCAAGATAATATCAAAGGTGTTTGGGAGAGAAATTATAGTGAGGGATGGAAGCAGATTTCATCATTTCAAGGAGGGCAAGTGTTCTTGCCTTGATTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

623

Amino Acids

69.55

Weight (kDa)

7.98

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 79 - 129 2e-06 PPR repeat family
PPR_2 PF13041 183 - 215 5.6e-07 PPR repeat family
PPR PF01535 186 - 215 1e-06 PPR repeat
PPR_2 PF13041 214 - 260 8.3e-10 PPR repeat family
PPR PF01535 217 - 246 2.9e-09 PPR repeat
E_motif PF20431 433 - 495 1e-13 E motif
DYW_deaminase PF14432 531 - 623 2.9e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016920)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g17040
malus_domestica MD15G1440600.v1.1
prunus_persica Prupe.1G582700_v2.0.a1 Prupe.1G582700_v2.0.a1
pyrus_communis pycom15g38840
rosa_chinensis RchiOBHm_Chr7g0220271
rosa_laevigata RLG00000002244
rosa_multiflora Rmu_sc0001136.1_g000028
rosa_roxburghii Rroxscaffold_3G00239660
rosa_rugosa Rorug07G0191700
rosa_samantha Rh7AG333700 Rh7BG323400 Rh7CG351300
rosa_wichuraiana Rw7G028150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 15, 1551
Acc16I TGCGCA 1 cut(s) 540
Acc36I ACCTGC 2 cut(s) 1085, 1457
AccB1I GGYRCC 1 cut(s) 1079
AccB7I CCANNNNNTGG 1 cut(s) 151
AccBSI CCGCTC 1 cut(s) 1672
AccII CGCG 1 cut(s) 398
AclI AACGTT 1 cut(s) 1534
AclWI GGATC 4 cut(s) 256, 1250, 1343, 1356
AcsI RAATTY 1 cut(s) 1735
AfaI GTAC 2 cut(s) 1145, 1374
AfiI CCNNNNNNNGG 2 cut(s) 151, 1362
AflIII ACRYGT 1 cut(s) 882
AgsI TTSAA 9 cut(s) 72, 318, 509, 748, 841, 908, 1343, 1484, 1837
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AluBI AGCT 5 cut(s) 333, 380, 413, 1463, 1589
AluI AGCT 5 cut(s) 333, 380, 413, 1463, 1589
Alw21I GWGCWC 2 cut(s) 415, 662
Alw26I GTCTC 5 cut(s) 127, 723, 769, 1046, 1556
AlwI GGATC 4 cut(s) 256, 1250, 1343, 1356
AlwNI CAGNNNCTG 1 cut(s) 1259
Ama87I CYCGRG 2 cut(s) 546, 1539
AoxI GGCC 7 cut(s) 33, 127, 390, 753, 981, 991, 1195
ApeKI GCWGC 7 cut(s) 267, 322, 333, 384, 578, 1442, 1463
ApoI RAATTY 1 cut(s) 1735
ArsI GACNNNNNNTTYG 4 cut(s) 443, 475, 1595, 1627
AseI ATTAAT 1 cut(s) 1130
AspLEI GCGC 3 cut(s) 400, 541, 1009
AspS9I GGNCC 2 cut(s) 33, 418
AsuHPI GGTGA 5 cut(s) 97, 124, 149, 279, 1724
AvaI CYCGRG 2 cut(s) 546, 1539
AvaII GGWCC 1 cut(s) 418
BamHI GGATCC 1 cut(s) 1348
BanI GGYRCC 1 cut(s) 1079
BanII GRGCYC 1 cut(s) 415
Bbv12I GWGCWC 2 cut(s) 415, 662
BbvI GCAGC 7 cut(s) 254, 309, 320, 396, 565, 1429, 1450
BccI CCATC 7 cut(s) 233, 784, 1156, 1355, 1432, 1703, 1808
BceAI ACGGC 3 cut(s) 1006, 1162, 1182
BcgI CGANNNNNNTGC 4 cut(s) 315, 349, 869, 903
BcoDI GTCTC 5 cut(s) 127, 723, 769, 1046, 1556
BfaI CTAG 3 cut(s) 800, 1379, 1559
BfmI CTRYAG 2 cut(s) 1084, 1464
BfoI RGCGCY 1 cut(s) 1010
BfuAI ACCTGC 2 cut(s) 1085, 1457
BglI GCCNNNNNGGC 1 cut(s) 1203
BisI GCNGC 9 cut(s) 268, 323, 334, 385, 579, 1221, 1443, 1464, 1673
BlsI GCNGC 9 cut(s) 269, 324, 335, 386, 580, 1222, 1444, 1465, 1674
Bme18I GGWCC 1 cut(s) 418
BmeT110I CYCGRG 2 cut(s) 546, 1539
BmgT120I GGNCC 2 cut(s) 33, 418
BmiI GGNNCC 4 cut(s) 34, 1081, 1248, 1350
BmsI GCATC 3 cut(s) 788, 890, 1284
Bpu10I CCTNAGC 1 cut(s) 414
BsaAI YACGTR 1 cut(s) 679
BsaI GGTCTC 1 cut(s) 1046
BsaWI WCCGGW 1 cut(s) 671
BsaXI ACNNNNNCTCC 4 cut(s) 402, 432, 1832, 1862
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 1362
Bse1I ACTGG 1 cut(s) 1383
Bse3DI GCAATG 1 cut(s) 1686
BseGI GGATG 5 cut(s) 744, 779, 905, 1714, 1819
BseLI CCNNNNNNNGG 2 cut(s) 151, 1362
BseMI GCAATG 1 cut(s) 1686
BseMII CTCAG 3 cut(s) 428, 932, 1215
BseNI ACTGG 1 cut(s) 1383
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 7 cut(s) 254, 309, 320, 396, 565, 1429, 1450
BsgI GTGCAG 1 cut(s) 1221
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 7 cut(s) 35, 129, 392, 755, 983, 993, 1197
BshNI GGYRCC 1 cut(s) 1079
BsiHKAI GWGCWC 2 cut(s) 415, 662
BsiHKCI CYCGRG 2 cut(s) 546, 1539
BsiSI CCGG 2 cut(s) 126, 672
BslFI GGGAC 1 cut(s) 1032
BslI CCNNNNNNNGG 2 cut(s) 151, 1362
BsmAI GTCTC 5 cut(s) 127, 723, 769, 1046, 1556
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 1032
BsmI GAATGC 5 cut(s) 475, 515, 1149, 1654, 1719
BsnI GGCC 7 cut(s) 35, 129, 392, 755, 983, 993, 1197
Bso31I GGTCTC 1 cut(s) 1046
BsoBI CYCGRG 2 cut(s) 546, 1539
Bsp1286I GDGCHC 2 cut(s) 415, 662
Bsp1407I TGTACA 1 cut(s) 1372
Bsp143I GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
BspANI GGCC 7 cut(s) 35, 129, 392, 755, 983, 993, 1197
BspCNI CTCAG 3 cut(s) 427, 933, 1216
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 4 cut(s) 34, 1081, 1248, 1350
BspMAI CTGCAG 2 cut(s) 1088, 1468
BspMI ACCTGC 2 cut(s) 1085, 1457
BspPI GGATC 4 cut(s) 256, 1250, 1343, 1356
BspT107I GGYRCC 1 cut(s) 1079
BspTNI GGTCTC 1 cut(s) 1046
BsrBI CCGCTC 1 cut(s) 1672
BsrDI GCAATG 1 cut(s) 1686
BsrGI TGTACA 1 cut(s) 1372
BsrI ACTGG 1 cut(s) 1383
BssMI GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
Bst4CI ACNGT 7 cut(s) 241, 664, 809, 856, 912, 1040, 1246
Bst6I CTCTTC 1 cut(s) 319
BstAUI TGTACA 1 cut(s) 1372
BstBAI YACGTR 1 cut(s) 679
BstDEI CTNAG 4 cut(s) 414, 941, 1192, 1224
BstF5I GGATG 5 cut(s) 744, 779, 905, 1714, 1819
BstFNI CGCG 1 cut(s) 398
BstH2I RGCGCY 1 cut(s) 1010
BstHHI GCGC 3 cut(s) 400, 541, 1009
BstKTI GATC 6 cut(s) 264, 651, 972, 1189, 1258, 1351
BstMAI GTCTC 5 cut(s) 127, 723, 769, 1046, 1556
BstMBI GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
BstMWI GCNNNNNNNGC 5 cut(s) 97, 688, 1004, 1203, 1669
BstNSI RCATGY 1 cut(s) 886
BstSFI CTRYAG 2 cut(s) 1084, 1464
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 7 cut(s) 254, 309, 320, 396, 565, 1429, 1450
BstX2I RGATCY 2 cut(s) 1255, 1348
BstYI RGATCY 2 cut(s) 1255, 1348
BsuRI GGCC 7 cut(s) 35, 129, 392, 755, 983, 993, 1197
BtsCI GGATG 5 cut(s) 744, 779, 905, 1714, 1819
BtsIMutI CAGTG 2 cut(s) 237, 660
BveI ACCTGC 2 cut(s) 1085, 1457
CaiI CAGNNNCTG 1 cut(s) 1259
CfoI GCGC 3 cut(s) 400, 541, 1009
Cfr13I GGNCC 2 cut(s) 33, 418
Csp6I GTAC 2 cut(s) 1144, 1373
CviQI GTAC 2 cut(s) 1144, 1373
DdeI CTNAG 4 cut(s) 414, 941, 1192, 1224
DpnI GATC 6 cut(s) 263, 650, 971, 1188, 1257, 1350
DpnII GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
DraI TTTAAA 2 cut(s) 718, 1126
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
EciI GGCGGA 2 cut(s) 609, 772
Ecl136II GAGCTC 1 cut(s) 413
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 1046
Eco47I GGWCC 1 cut(s) 418
Eco53kI GAGCTC 1 cut(s) 413
Eco88I CYCGRG 2 cut(s) 546, 1539
EcoICRI GAGCTC 1 cut(s) 413
EcoT22I ATGCAT 2 cut(s) 905, 1299
EcoT38I GRGCYC 1 cut(s) 415
Esp3I CGTCTC 1 cut(s) 127
FaqI GGGAC 1 cut(s) 1032
FauI CCCGC 2 cut(s) 855, 1057
Fnu4HI GCNGC 9 cut(s) 268, 323, 334, 385, 579, 1221, 1443, 1464, 1673
FokI GGATG 5 cut(s) 751, 766, 912, 1721, 1826
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 9 cut(s) 268, 323, 334, 385, 579, 1221, 1443, 1464, 1673
FspAI RTGCGCAY 1 cut(s) 540
FspBI CTAG 3 cut(s) 800, 1379, 1559
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 3 cut(s) 399, 540, 1008
GluI GCNGC 9 cut(s) 268, 323, 334, 385, 579, 1221, 1443, 1464, 1673
HaeII RGCGCY 1 cut(s) 1010
HaeIII GGCC 7 cut(s) 35, 129, 392, 755, 983, 993, 1197
HapII CCGG 2 cut(s) 126, 672
HhaI GCGC 3 cut(s) 400, 541, 1009
Hin6I GCGC 3 cut(s) 398, 539, 1007
HinP1I GCGC 3 cut(s) 398, 539, 1007
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 3 cut(s) 478, 833, 1339
HpaI GTTAAC 1 cut(s) 1029
HpaII CCGG 2 cut(s) 126, 672
HphI GGTGA 5 cut(s) 97, 124, 149, 279, 1724
Hpy166II GTNNAC 2 cut(s) 820, 1029
Hpy188I TCNGA 3 cut(s) 590, 1431, 1725
Hpy188III TCNNGA 5 cut(s) 63, 466, 546, 1539, 1541
Hpy8I GTNNAC 2 cut(s) 820, 1029
Hpy99I CGWCG 1 cut(s) 1040
HpyAV CCTTC 3 cut(s) 401, 695, 1103
HpyCH4III ACNGT 7 cut(s) 241, 664, 809, 856, 912, 1040, 1246
HpyCH4IV ACGT 3 cut(s) 678, 1061, 1534
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 688, 1004, 1203, 1669
HpyF3I CTNAG 4 cut(s) 414, 941, 1192, 1224
HpySE526I ACGT 3 cut(s) 678, 1061, 1534
HspAI GCGC 3 cut(s) 398, 539, 1007
KspAI GTTAAC 1 cut(s) 1029
Kzo9I GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
LmnI GCTCC 6 cut(s) 173, 410, 518, 657, 1165, 1660
Lsp1109I GCAGC 7 cut(s) 254, 309, 320, 396, 565, 1429, 1450
LweI GCATC 3 cut(s) 788, 890, 1284
MaeI CTAG 3 cut(s) 800, 1379, 1559
MaeII ACGT 3 cut(s) 678, 1061, 1534
MaeIII GTNAC 6 cut(s) 463, 556, 674, 1057, 1570, 1699
MalI GATC 6 cut(s) 263, 650, 971, 1188, 1257, 1350
MbiI CCGCTC 1 cut(s) 1672
MboI GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
MboII GAAGA 5 cut(s) 306, 339, 1424, 1649, 1744
MfeI CAATTG 1 cut(s) 1343
MflI RGATCY 2 cut(s) 1255, 1348
MhlI GDGCHC 2 cut(s) 415, 662
MluCI AATT 5 cut(s) 1127, 1310, 1343, 1735, 1800
MlyI GAGTC 1 cut(s) 842
MmeI TCCRAC 3 cut(s) 687, 1376, 1451
Mph1103I ATGCAT 2 cut(s) 905, 1299
MseI TTAA 5 cut(s) 717, 927, 1028, 1125, 1130
MslI CAYNNNNRTG 2 cut(s) 426, 521
MspA1I CMGCKG 3 cut(s) 267, 850, 1223
MspI CCGG 2 cut(s) 126, 672
MunI CAATTG 1 cut(s) 1343
Mva1269I GAATGC 5 cut(s) 475, 515, 1149, 1654, 1719
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 5 cut(s) 97, 688, 1004, 1203, 1669
NdeII GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
NlaIV GGNNCC 4 cut(s) 34, 1081, 1248, 1350
NmuCI GTSAC 2 cut(s) 463, 1057
NsbI TGCGCA 1 cut(s) 540
NsiI ATGCAT 2 cut(s) 905, 1299
NspI RCATGY 1 cut(s) 886
PaeR7I CTCGAG 1 cut(s) 1539
PceI AGGCCT 1 cut(s) 392
PciI ACATGT 1 cut(s) 882
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PctI GAATGC 5 cut(s) 475, 515, 1149, 1654, 1719
PfeI GAWTC 2 cut(s) 478, 1339
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 9 cut(s) 269, 324, 335, 386, 580, 1222, 1444, 1465, 1674
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
Ppu21I YACGTR 1 cut(s) 679
PscI ACATGT 1 cut(s) 882
PshBI ATTAAT 1 cut(s) 1130
PsiI TTATAA 2 cut(s) 15, 1551
Psp124BI GAGCTC 1 cut(s) 415
Psp1406I AACGTT 1 cut(s) 1534
PspN4I GGNNCC 4 cut(s) 34, 1081, 1248, 1350
PspPI GGNCC 2 cut(s) 33, 418
PstI CTGCAG 2 cut(s) 1088, 1468
PstNI CAGNNNCTG 1 cut(s) 1259
PsuI RGATCY 2 cut(s) 1255, 1348
RsaI GTAC 2 cut(s) 1145, 1374
RsaNI GTAC 2 cut(s) 1144, 1373
RseI CAYNNNNRTG 2 cut(s) 426, 521
SacI GAGCTC 1 cut(s) 415
SaqAI TTAA 5 cut(s) 717, 927, 1028, 1125, 1130
SatI GCNGC 9 cut(s) 268, 323, 334, 385, 579, 1221, 1443, 1464, 1673
Sau3AI GATC 6 cut(s) 261, 648, 969, 1186, 1255, 1348
Sau96I GGNCC 2 cut(s) 33, 418
SchI GAGTC 1 cut(s) 842
SduI GDGCHC 2 cut(s) 415, 662
SfaNI GCATC 3 cut(s) 788, 890, 1284
SfcI CTRYAG 2 cut(s) 1084, 1464
Sfr274I CTCGAG 1 cut(s) 1539
SinI GGWCC 1 cut(s) 418
SlaI CTCGAG 1 cut(s) 1539
SmiMI CAYNNNNRTG 2 cut(s) 426, 521
SmlI CTYRAG 1 cut(s) 1539
SmoI CTYRAG 1 cut(s) 1539
Sse9I AATT 5 cut(s) 1127, 1310, 1343, 1735, 1800
SseBI AGGCCT 1 cut(s) 392
SspMI CTAG 3 cut(s) 800, 1379, 1559
SstI GAGCTC 1 cut(s) 415
StuI AGGCCT 1 cut(s) 392
TaaI ACNGT 7 cut(s) 241, 664, 809, 856, 912, 1040, 1246
TaiI ACGT 3 cut(s) 681, 1064, 1537
TaqI TCGA 5 cut(s) 292, 879, 1011, 1212, 1540
TaqII GACCGA 1 cut(s) 1151
TasI AATT 5 cut(s) 1127, 1310, 1343, 1735, 1800
TatI WGTACW 1 cut(s) 1372
TauI GCSGC 2 cut(s) 1223, 1675
TfiI GAWTC 2 cut(s) 478, 1339
Tru1I TTAA 5 cut(s) 717, 927, 1028, 1125, 1130
Tru9I TTAA 5 cut(s) 717, 927, 1028, 1125, 1130
TscAI CASTG 2 cut(s) 244, 667
TseFI GTSAC 2 cut(s) 463, 1057
TseI GCWGC 7 cut(s) 267, 322, 333, 384, 578, 1442, 1463
Tsp45I GTSAC 2 cut(s) 463, 1057
TspDTI ATGAA 7 cut(s) 470, 642, 1054, 1230, 1490, 1545, 1817
TspRI CASTG 2 cut(s) 244, 667
Van91I CCANNNNNTGG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 418
VspI ATTAAT 1 cut(s) 1130
XapI RAATTY 1 cut(s) 1735
XceI RCATGY 1 cut(s) 886
XhoI CTCGAG 1 cut(s) 1539
XspI CTAG 3 cut(s) 800, 1379, 1559
Zsp2I ATGCAT 2 cut(s) 905, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.