MD15G1443100.v1.1

Protein NRT1 PTR FAMILY 4.3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009913
Physical Location & Seq
Forward (+)
54322869 .. 54325021
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1443100.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGCAGGGTTTCATATTACTGTCAGTCCAAGCTCACGTTCCGCAGCTAAAACCACCGCCATGCAACGTTATTAACGGAGAGGAGTGTGTAGAGGCAAAAGGGTTCAAGTCATTAATCTTCTTTGTGGCACTTTACTTGGTGGCATTGGGGAGTGGCTGTGTCAAGCCAAACATGATCGCTCACGGGGCTGACCAGTTCAACCCAGATGACCCAAAGCAGTACAAGAAACTCTCCACCTACTTCAACGCTGCCTACTTTGCCTTTTCCATGGGTGAACTCGTCGCCCTCACTCTTCTTGTGTGGGTCCAAACTCACTCTGGAATGGATGTTGGCTTTGGAGTCTCAGCAGCCGCCATGGCAATGGGATTAATCAGCTTGGTTTCCGGTACCCTATATTACAGGAACAAGCGACCTCAAGGAAGCATACTCACTCCCATTGCTCAAGTTTTTGTGGCTGCAATGCTAAAGAGAAAGCAGGTTTGTCCATCTAATCCACAGTTGCTCCATGGAAACCAAAACAATGTGCTGCATGACAATACTACTTTCTTTTCTGACGCTGCCGGCTTTCTTCACACTGAAAAGTTCAGGTTCTTGGACAAGGCTTGCATTAAAATTCAAGGTGGCACTAATTCAAAGGAAAGCCCGTGGAGATTGTGCACCGTTACTCAAGTGAAGCAAGTCAAAATACTGCTTTCAGTTATTCCAATTTTTGCTTCCACCATAATTTTCAACACAATTTTGGCTCAGCTCCAAACTTTCTCAGTCCAACAAGGAAGTATGATGGACACCAAGCTCACCAAATCCTTCCATATCCCTCCAGCTTCACTCCAATCCATCCCCTACATCATTCTCATCGCCCTCGTCCCTCTCTACGACACTTTCTTTGTTCCCTTTGCTCGAAAATTCACCGGTCACAATTCTGGCATCTCTCCGTTAATGAGAATAGGGTTTGGACTCTTTTTTGCAACATTTTCGATGATCGCGGCTGCCATCATGGAGGAAAAGAGAAGGGATGCAGCTGTGCATTCGAACAAAATAATATCCATTTTTTGGATCACCCCACAATTCTTGATCTTTGGATTATCAGAAATGTTCACTGCCGTAGGCCTCGTTGAGTTCTTTTACAAACAATCCTTGAAAGGGATGCAAGCATTTTTAACTGCCTTGACCTATTGCTCATATTCGTTTGGGTTTTACCTGAGCTCTCTACTTGTCTCTCTGGTGAATAAGATCACAACCTCATCAGGTGGTGGTGGTTGGCTGGGTGACAATAATCTCAACAGAGACAGACTGGACCTTTTCTATTGGTTGCTGGCAGGCCTCAGCTTCCTCAACTTCCTCAACTATCTATTTTGGTCTCATTGGTACTCTCGCACCCCGTCATCTTCATCAGCACCAAGTGCACTACCCCAGCTGCATGCTCATGAGACTACTTGCATGGAGGATTATAAACATCATAGACTGGATGATAATATACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0016020 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

494

Amino Acids

54.88

Weight (kDa)

9.1

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 3 - 420 1.3e-88 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G59740
fragaria_vesca FvH4_5g27820 FvH4_5g27820 FvH4_5g27820
malus_domestica MD08G1248200.v1.1 MD15G1443100.v1.1
prunus_persica Prupe.1G585400_v2.0.a1
pyrus_communis pycom08g21500 pycom15g39080
rosa_chinensis RchiOBHm_Chr7g0219651
rosa_laevigata RLG00000002280
rosa_multiflora Rmu_sc0001828.1_g000003
rosa_roxburghii Rroxscaffold_3G00239960
rosa_rugosa Rorug07G0186700
rosa_samantha Rh1BG165700 Rh1DG196300 Rh7AG330500 Rh7BG320300 Rh7CG347800 Rh7DG325800
rosa_wichuraiana Rw7G027820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1449
Acc36I ACCTGC 1 cut(s) 466
Acc65I GGTACC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 1050
AccII CGCG 1 cut(s) 983
AciI CCGC 4 cut(s) 41, 56, 351, 983
AclI AACGTT 1 cut(s) 66
AclWI GGATC 1 cut(s) 1061
AcsI RAATTY 2 cut(s) 612, 902
AdeI CACNNNGTG 1 cut(s) 1400
AfaI GTAC 3 cut(s) 221, 388, 1367
AfiI CCNNNNNNNGG 2 cut(s) 1050, 1140
AgeI ACCGGT 1 cut(s) 908
AgsI TTSAA 7 cut(s) 106, 199, 244, 617, 633, 730, 1138
AjuI GAANNNNNNNTTGG 4 cut(s) 21, 53, 697, 729
Alw21I GWGCWC 3 cut(s) 659, 1205, 1405
Alw26I GTCTC 5 cut(s) 346, 1219, 1278, 1362, 1421
Alw44I GTGCAC 2 cut(s) 655, 1401
AlwI GGATC 1 cut(s) 1061
AoxI GGCC 2 cut(s) 1105, 1318
ApaLI GTGCAC 2 cut(s) 655, 1401
ApeKI GCWGC 9 cut(s) 43, 248, 347, 455, 526, 557, 986, 1016, 1414
ApoI RAATTY 2 cut(s) 612, 902
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AseI ATTAAT 2 cut(s) 113, 368
AsiGI ACCGGT 1 cut(s) 908
Asp718I GGTACC 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 304, 1294
AsuHPI GGTGA 6 cut(s) 284, 787, 898, 1048, 1234, 1277
AsuII TTCGAA 1 cut(s) 1028
AvaII GGWCC 2 cut(s) 304, 1294
BaeGI GKGCMC 2 cut(s) 659, 1405
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 1205
Bbv12I GWGCWC 3 cut(s) 659, 1205, 1405
BbvCI CCTCAGC 1 cut(s) 1322
BbvI GCAGC 9 cut(s) 55, 235, 359, 442, 513, 544, 973, 1028, 1401
BccI CCATC 4 cut(s) 493, 775, 842, 998
BceAI ACGGC 1 cut(s) 1085
BcgI CGANNNNNNTGC 2 cut(s) 954, 988
BcoDI GTCTC 5 cut(s) 346, 1219, 1278, 1362, 1421
BfuAI ACCTGC 1 cut(s) 466
BglI GCCNNNNNGGC 1 cut(s) 356
BlpI GCTNAGC 1 cut(s) 744
Bme18I GGWCC 2 cut(s) 304, 1294
BmgT120I GGNCC 2 cut(s) 304, 1294
BmiI GGNNCC 2 cut(s) 305, 388
BmsI GCATC 3 cut(s) 933, 1003, 1134
BpmI CTGGAG 1 cut(s) 801
Bpu10I CCTNAGC 2 cut(s) 1199, 1322
Bpu1102I GCTNAGC 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 1028
BpuEI CTTGAG 3 cut(s) 399, 426, 651
BsaI GGTCTC 1 cut(s) 1362
BsaJI CCNNGG 4 cut(s) 267, 354, 505, 644
BsaWI WCCGGW 2 cut(s) 383, 908
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 486, 516
Bsc4I CCNNNNNNNGG 2 cut(s) 1050, 1140
Bse118I RCCGGY 2 cut(s) 560, 908
Bse1I ACTGG 3 cut(s) 193, 1296, 1467
Bse3DI GCAATG 3 cut(s) 366, 435, 465
BseDI CCNNGG 4 cut(s) 267, 354, 505, 644
BseGI GGATG 5 cut(s) 331, 834, 1018, 1149, 1471
BseLI CCNNNNNNNGG 2 cut(s) 1050, 1140
BseMI GCAATG 3 cut(s) 366, 435, 465
BseMII CTCAG 5 cut(s) 357, 758, 774, 1190, 1336
BseNI ACTGG 3 cut(s) 193, 1296, 1467
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 2 cut(s) 659, 1405
BseXI GCAGC 9 cut(s) 55, 235, 359, 442, 513, 544, 973, 1028, 1401
BseYI CCCAGC 2 cut(s) 1261, 1410
Bsh1236I CGCG 1 cut(s) 983
BshFI GGCC 2 cut(s) 1107, 1320
BshNI GGYRCC 1 cut(s) 386
BshTI ACCGGT 1 cut(s) 908
BsiHKAI GWGCWC 3 cut(s) 659, 1205, 1405
BsiSI CCGG 3 cut(s) 384, 561, 909
BslFI GGGAC 1 cut(s) 848
BslI CCNNNNNNNGG 2 cut(s) 1050, 1140
BsmAI GTCTC 5 cut(s) 346, 1219, 1278, 1362, 1421
BsmFI GGGAC 1 cut(s) 848
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 2 cut(s) 1107, 1320
Bso31I GGTCTC 1 cut(s) 1362
Bsp119I TTCGAA 1 cut(s) 1028
Bsp1286I GDGCHC 3 cut(s) 659, 1205, 1405
Bsp143I GATC 5 cut(s) 174, 978, 1053, 1071, 1230
Bsp1720I GCTNAGC 1 cut(s) 744
Bsp19I CCATGG 3 cut(s) 267, 354, 505
BspACI CCGC 4 cut(s) 41, 56, 351, 983
BspANI GGCC 2 cut(s) 1107, 1320
BspCNI CTCAG 5 cut(s) 356, 757, 773, 1191, 1335
BspFNI CGCG 1 cut(s) 983
BspHI TCATGA 1 cut(s) 1423
BspLI GGNNCC 2 cut(s) 305, 388
BspMI ACCTGC 1 cut(s) 466
BspPI GGATC 1 cut(s) 1061
BspT104I TTCGAA 1 cut(s) 1028
BspT107I GGYRCC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 1362
BsrDI GCAATG 3 cut(s) 366, 435, 465
BsrFI RCCGGY 2 cut(s) 560, 908
BsrI ACTGG 3 cut(s) 193, 1296, 1467
BssAI RCCGGY 2 cut(s) 560, 908
BssECI CCNNGG 4 cut(s) 267, 354, 505, 644
BssMI GATC 5 cut(s) 174, 978, 1053, 1071, 1230
BssT1I CCWWGG 3 cut(s) 267, 354, 505
Bst4CI ACNGT 3 cut(s) 21, 498, 661
Bst6I CTCTTC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 1400
BstBI TTCGAA 1 cut(s) 1028
BstC8I GCNNGC 6 cut(s) 562, 604, 1149, 1314, 1318, 1419
BstDEI CTNAG 5 cut(s) 343, 744, 760, 1199, 1322
BstDSI CCRYGG 4 cut(s) 267, 354, 505, 644
BstF5I GGATG 5 cut(s) 331, 834, 1018, 1149, 1471
BstFNI CGCG 1 cut(s) 983
BstKTI GATC 5 cut(s) 177, 981, 1056, 1074, 1233
BstMAI GTCTC 5 cut(s) 346, 1219, 1278, 1362, 1421
BstMBI GATC 5 cut(s) 174, 978, 1053, 1071, 1230
BstMWI GCNNNNNNNGC 4 cut(s) 185, 257, 356, 1400
BstNSI RCATGY 1 cut(s) 1421
BstSLI GKGCMC 2 cut(s) 659, 1405
BstUI CGCG 1 cut(s) 983
BstV1I GCAGC 9 cut(s) 55, 235, 359, 442, 513, 544, 973, 1028, 1401
BstXI CCANNNNNNTGG 1 cut(s) 361
BsuRI GGCC 2 cut(s) 1107, 1320
BtgI CCRYGG 4 cut(s) 267, 354, 505, 644
BtgZI GCGATG 1 cut(s) 838
BtsCI GGATG 5 cut(s) 331, 834, 1018, 1149, 1471
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 2 cut(s) 573, 1095
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 6 cut(s) 562, 604, 1149, 1314, 1318, 1419
CciI TCATGA 1 cut(s) 1423
Cfr10I RCCGGY 2 cut(s) 560, 908
Cfr13I GGNCC 2 cut(s) 304, 1294
CseI GACGC 1 cut(s) 563
Csp6I GTAC 3 cut(s) 220, 387, 1366
CspAI ACCGGT 1 cut(s) 908
CspCI CAANNNNNGTGG 2 cut(s) 1050, 1085
CviQI GTAC 3 cut(s) 220, 387, 1366
DdeI CTNAG 5 cut(s) 343, 744, 760, 1199, 1322
DpnI GATC 5 cut(s) 176, 980, 1055, 1073, 1232
DpnII GATC 5 cut(s) 174, 978, 1053, 1071, 1230
DraIII CACNNNGTG 1 cut(s) 1400
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 1203
Eco130I CCWWGG 3 cut(s) 267, 354, 505
Eco147I AGGCCT 2 cut(s) 1107, 1320
Eco24I GRGCYC 1 cut(s) 1205
Eco31I GGTCTC 1 cut(s) 1362
Eco47I GGWCC 2 cut(s) 304, 1294
Eco53kI GAGCTC 1 cut(s) 1203
EcoICRI GAGCTC 1 cut(s) 1203
EcoT14I CCWWGG 3 cut(s) 267, 354, 505
EcoT38I GRGCYC 1 cut(s) 1205
ErhI CCWWGG 3 cut(s) 267, 354, 505
FaqI GGGAC 1 cut(s) 848
FokI GGATG 5 cut(s) 338, 821, 1025, 1156, 1478
FriOI GRGCYC 1 cut(s) 1205
GsaI CCCAGC 2 cut(s) 1265, 1414
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 2 cut(s) 1107, 1320
HapII CCGG 3 cut(s) 384, 561, 909
HgaI GACGC 1 cut(s) 563
HinfI GANTC 2 cut(s) 339, 954
HpaII CCGG 3 cut(s) 384, 561, 909
HphI GGTGA 6 cut(s) 284, 787, 898, 1048, 1234, 1277
Hpy166II GTNNAC 4 cut(s) 275, 657, 1095, 1403
Hpy188I TCNGA 2 cut(s) 553, 1087
Hpy188III TCNNGA 3 cut(s) 318, 1069, 1424
Hpy8I GTNNAC 4 cut(s) 275, 657, 1095, 1403
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 2 cut(s) 814, 1002
HpyCH4III ACNGT 3 cut(s) 21, 498, 661
HpyCH4IV ACGT 2 cut(s) 36, 66
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 257, 356, 1400
HpyF3I CTNAG 5 cut(s) 343, 744, 760, 1199, 1322
HpySE526I ACGT 2 cut(s) 36, 66
KpnI GGTACC 1 cut(s) 390
KroI GCCGGC 1 cut(s) 560
KroNI GCCGGC 1 cut(s) 562
Kzo9I GATC 5 cut(s) 174, 978, 1053, 1071, 1230
LmnI GCTCC 2 cut(s) 507, 753
Lsp1109I GCAGC 9 cut(s) 55, 235, 359, 442, 513, 544, 973, 1028, 1401
LweI GCATC 3 cut(s) 933, 1003, 1134
MaeII ACGT 2 cut(s) 36, 66
MaeIII GTNAC 3 cut(s) 661, 911, 1265
MalI GATC 5 cut(s) 176, 980, 1055, 1073, 1232
MboI GATC 5 cut(s) 174, 978, 1053, 1071, 1230
MboII GAAGA 4 cut(s) 109, 284, 560, 1377
MhlI GDGCHC 3 cut(s) 659, 1205, 1405
MluCI AATT 8 cut(s) 612, 628, 705, 723, 735, 902, 916, 1064
MlyI GAGTC 2 cut(s) 348, 948
MmeI TCCRAC 1 cut(s) 790
MroNI GCCGGC 1 cut(s) 560
MseI TTAA 6 cut(s) 72, 113, 368, 609, 935, 1157
MslI CAYNNNNRTG 3 cut(s) 58, 359, 1422
MspA1I CMGCKG 2 cut(s) 1019, 1414
MspI CCGG 3 cut(s) 384, 561, 909
Mva1269I GAATGC 1 cut(s) 1024
MvnI CGCG 1 cut(s) 983
MwoI GCNNNNNNNGC 4 cut(s) 185, 257, 356, 1400
NaeI GCCGGC 1 cut(s) 562
NcoI CCATGG 3 cut(s) 267, 354, 505
NdeII GATC 5 cut(s) 174, 978, 1053, 1071, 1230
NgoMIV GCCGGC 1 cut(s) 560
NlaIV GGNNCC 2 cut(s) 305, 388
NmuCI GTSAC 2 cut(s) 911, 1265
NspI RCATGY 1 cut(s) 1421
NspV TTCGAA 1 cut(s) 1028
PaeI GCATGC 1 cut(s) 1421
PagI TCATGA 1 cut(s) 1423
PceI AGGCCT 2 cut(s) 1107, 1320
PctI GAATGC 1 cut(s) 1024
PdiI GCCGGC 1 cut(s) 562
PflMI CCANNNNNTGG 1 cut(s) 1050
PinAI ACCGGT 1 cut(s) 908
PleI GAGTC 2 cut(s) 347, 948
PpsI GAGTC 2 cut(s) 347, 948
PshBI ATTAAT 2 cut(s) 113, 368
PsiI TTATAA 1 cut(s) 1449
Psp124BI GAGCTC 1 cut(s) 1205
Psp1406I AACGTT 1 cut(s) 66
PspFI CCCAGC 2 cut(s) 1261, 1410
PspN4I GGNNCC 2 cut(s) 305, 388
PspPI GGNCC 2 cut(s) 304, 1294
PvuII CAGCTG 2 cut(s) 1019, 1414
RsaI GTAC 3 cut(s) 221, 388, 1367
RsaNI GTAC 3 cut(s) 220, 387, 1366
RseI CAYNNNNRTG 3 cut(s) 58, 359, 1422
SacI GAGCTC 1 cut(s) 1205
SaqAI TTAA 6 cut(s) 72, 113, 368, 609, 935, 1157
Sau3AI GATC 5 cut(s) 174, 978, 1053, 1071, 1230
Sau96I GGNCC 2 cut(s) 304, 1294
SchI GAGTC 2 cut(s) 348, 948
SduI GDGCHC 3 cut(s) 659, 1205, 1405
SfaNI GCATC 3 cut(s) 933, 1003, 1134
SfuI TTCGAA 1 cut(s) 1028
SinI GGWCC 2 cut(s) 304, 1294
SmiMI CAYNNNNRTG 3 cut(s) 58, 359, 1422
SmlI CTYRAG 3 cut(s) 414, 441, 666
SmoI CTYRAG 3 cut(s) 414, 441, 666
SphI GCATGC 1 cut(s) 1421
Sse9I AATT 8 cut(s) 612, 628, 705, 723, 735, 902, 916, 1064
SseBI AGGCCT 2 cut(s) 1107, 1320
SsiI CCGC 4 cut(s) 41, 56, 351, 983
SstI GAGCTC 1 cut(s) 1205
StuI AGGCCT 2 cut(s) 1107, 1320
StyI CCWWGG 3 cut(s) 267, 354, 505
TaaI ACNGT 3 cut(s) 21, 498, 661
TaiI ACGT 2 cut(s) 39, 69
TaqI TCGA 3 cut(s) 898, 974, 1028
TasI AATT 8 cut(s) 612, 628, 705, 723, 735, 902, 916, 1064
TatI WGTACW 1 cut(s) 219
TauI GCSGC 2 cut(s) 353, 986
Tru1I TTAA 6 cut(s) 72, 113, 368, 609, 935, 1157
Tru9I TTAA 6 cut(s) 72, 113, 368, 609, 935, 1157
TscAI CASTG 2 cut(s) 580, 1102
TseFI GTSAC 2 cut(s) 911, 1265
TseI GCWGC 9 cut(s) 43, 248, 347, 455, 526, 557, 986, 1016, 1414
Tsp45I GTSAC 2 cut(s) 911, 1265
TspDTI ATGAA 1 cut(s) 1377
TspGWI ACGGA 2 cut(s) 90, 921
TspRI CASTG 2 cut(s) 580, 1102
Van91I CCANNNNNTGG 1 cut(s) 1050
VneI GTGCAC 2 cut(s) 655, 1401
VpaK11BI GGWCC 2 cut(s) 304, 1294
VspI ATTAAT 2 cut(s) 113, 368
XapI RAATTY 2 cut(s) 612, 902
XceI RCATGY 1 cut(s) 1421
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.