MD16G1108100.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014515
Physical Location & Seq
Reverse (-)
7554894 .. 7560460
5567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1108100.v1.1.491

Sequence Viewer

Length: 2790 bp
ATGGGTTTCTCCTTCTTCCCGTTCATAATTTATCTTTGCTTTATATCATCTGTTATTTCCCAGGTTCAAGATTTTGTGAGCATAGATTGTGGAGGGACAAGTAACTACACTGACACAAGCACTGGACTGGCATGGATTTCAGACGTGGGACTCATGTCCCAGGGAAAATCAGTCCAGGTCGAAAACTCTAATGGAAACTTGTTGCAGTTTCAGAGACGCAGGGATTTTCCCATAGACAGCAAGAAGTACTGCTATACATTGAACACTGTGGAGAGGAGGAGGTATTTAGTCCGGGCAACATTTCAGTACGGCAGTCTCAAAAGTGAAGACACATATCCGAAATTCGAGCTTTACTTGGATGCCACGCAGTGGGCAATCGTGACTATTTTTGATGCTTCAAGAACATATGTGAATGAGATGATCATTAGGGCTTCGTCGGATTCTGTCGATGTGTGTATATGTTGCGCAACAACTGGGTTTCCGTTTATATCGACTTTGGAGCTGAGGCCTTTGAATCTTTCGATGTATGCCACGGACTATGAGGATGAGTTTTTCTTGAAAGTTGCAGCTAGGGTGAATTTTGGTGCTCCAAGCAAAGATGCTATAAGGTACCCGGATGATCCATATGACAGAATTTGGGATTCTGATCTTGTGAAAAGGCAGAATTATTTGGTTGGAGTGGCACCAGGTACAGAAAGAATCAGTACATCAAGGGATGTAGACACAAGGAGTAGAGAGTACCCGCCTGTTAAAGTAATGCAGACCGCGGTAGTTGGCACAAAGGGAGTGCTGAGCTACAGATTAAATCTGGATGGCTTCCCAGGAATGGGTCGAGCTTATGCTTACTTTGCGGAAATTGAGGATTTAGGCGCAAATGAGAACCGGAAATTCAAGTTGGAGCAACCTTTCTCGGCTGACTATAATAGTGCAGTGGTAAATATCGCTGAGAATGCAAATGGGGACTACAGGCTTTATGAGCCGAGCTACATGAATGTGAGTCTGGAGTTTGTTCTATCATTTGCCTTTGTTAGGACCCCTGATTCTACGCGAGGGCCGCTTCTAAATGCAATGGAGATAAGTAAATATGTTCAAATTGCTGCAAAGACCGATAGGCGAGATTTGGCGGTTCTGAATGTGCTTCACTTCATGGCTGCTGAAAGTCTATCAGTAGATGAAGGTGATCCTTGTGCTCCAACTCCGTGGGAATGGGTGAATTGTAGCTCCACAGCACCACCAAGAATCACAAAAATTGACCTATCAGGACAGAATGTGAAGGGTGAAATCCCCGTGGAGCTTAAAAACCTGCAGGATTTGACAGAGTTGTGGTTGGGTGGTAACTACCTTACCGGACAATTTCCTGACATCAGTAATCTTATCAATTTAAAGATTCTGCATCTGGAGAACAACAAATTGAGTGGTTCGGTACCTTCTTACCTGGCTAGCTTGCCAAGCTTACAAGAACTGTACATACAGAACAACTCTTTCAGTGGGGAAATTCCGGCAGGACTGTTAAGGGGGAAAATCACATTTAAATATGAATATAATATTGGACTACGTAAGAGGGAACGAAAACAGAAGCATTTCAAGTTGATAATTGGAATTTCTGTTGGAGTAGTTGCAACTGTATCATTGTTATTATTAGGGACTCTATTGATATTGCGTAATCTTCAAAGAAAGTCATCTTTTCAGAGAAGTAATGGAAAAGGTGACTCGCTGCGTGCCAGCACCAAGCCTTCAACTGGCTATTCAATTTCTCGTATGGATGAAGGCATAGCTTGCTACATTACTCTTCCTGATCTGGAAGAAGCGACTAGCAGCTTTTCAAAAAGAATTGGAAAAGGAAGCTTTGGGTCTGTATATTATGGAAAGATGAAAGATGGAAAGGAGATAGCAGTTAAAATGATGGCAGAATCATCTACCCACATGAATAAGCAATTTGTAACTGAGGTAGCACTCTTGTCACGTATTCATCATCGGAACTTGGTTCCTCTAACTGGATATTGCGAAGAAGAACATCAATGCATTCTGGTTTACGAGTATATGCACAATGGAACCTTACGCGATCACATACACGGTTCTACCACCCAGAAGCACTTAGACTGGCAAACTCGCCTTCGCATTGCAGAAGATGCAGCTAAAGGTCTCGAGTATTTACACACTGGTTGCAACCCTAGTATCATCCACCGGGACGTGAAAACAAGCAACATTCTCTTAGACATAAACATGAGAGCAAAAGTGTCGGATTTCGGATTGTCTAGGCAAGCAGAAGAGGATGTAACGCATCTATCAAGCGTGGCTCGGGGAACAGTAGGCTACCTTGATCCTGAGTATTATGCTAGTCAGCAATTAACTGAGAAAAGTGATGTGTATAGTTTTGGGGTTGTTCTTTTGGAGCTGATTTCAGGAAAAAAGCCAGTTTCAACAGAAGACTTTGGTTCTGAGTTGAACATTGTTCACTGGGCAAGATCCTTGATTCTTAAAGGAGATGTTGAAAGCATCATAGATCCTATCCTAGCAGGAAGTGTGAAAGTTGAGTCCATGTGGAGAATTGCTGAAGTAGCAATCCAGTGTGTTGAGCAACATGGTGTTTCGAGGCCAAGAATGCAGGAGATAATCGTGGCCGTTCAAGATGCTATGAAGATTGAAAAGGGCAGTGAATCCAACCAGAAAATATCACCCAGCAGTAGCAGTTCAAAAGCACAACCCTCCAGGAAAACTTTGCTAACAAGTTTTCTGGAAATTGAGGGCCCTGATTTGTCCGAGGATTGTTTAGTCCCATCTGCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_4 LRR_8 Malectin_like Pkinase_Tyr

Protein Analysis

930

Amino Acids

103.94

Weight (kDa)

6.06

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 28 - 361 6.8e-81 Malectin-like domain
LRR_8 PF13855 438 - 494 1.4e-06 Leucine rich repeat
LRR_4 PF12799 460 - 493 2.2e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 607 - 871 1.7e-50 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 608 - 810 1.2e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014515)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67720
fragaria_vesca FvH4_4g24900 FvH4_4g24900 FvH4_4g24900
malus_domestica MD13G1108000.v1.1 MD16G1108100.v1.1
prunus_persica Prupe.1G238400_v2.0.a1 Prupe.1G238400_v2.0.a1
pyrus_communis pycom16g09210
rosa_chinensis RchiOBHm_Chr4g0431961
rosa_laevigata RLG00000006871
rosa_multiflora Rmu_sc0004965.1_g000014
rosa_roxburghii Rroxscaffold_5G00373400
rosa_rugosa Rorug04G0253600 Rorug04G0253700
rosa_samantha Rh4BG317900
rosa_wichuraiana Rw4G027040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 466
Acc36I ACCTGC 1 cut(s) 1311
Acc65I GGTACC 2 cut(s) 609, 1423
AccB1I GGYRCC 3 cut(s) 609, 682, 1423
AccB7I CCANNNNNTGG 1 cut(s) 369
AccI GTMKAC 1 cut(s) 720
AccII CGCG 3 cut(s) 767, 1048, 2061
AciI CCGC 6 cut(s) 743, 765, 767, 851, 1055, 1124
AclWI GGATC 5 cut(s) 614, 1175, 2315, 2460, 2498
AcoI YGGCCR 1 cut(s) 2619
AcsI RAATTY 6 cut(s) 341, 577, 633, 887, 1494, 1599
AcuI CTGAAG 1 cut(s) 2574
AdeI CACNNNGTG 1 cut(s) 369
AfaI GTAC 8 cut(s) 248, 308, 611, 691, 706, 740, 1425, 1466
AfiI CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1029, 1739, 1994
AjiI CACGTC 2 cut(s) 145, 2191
AjnI CCWGG 7 cut(s) 60, 159, 174, 685, 820, 1434, 2708
AjuI GAANNNNNNNTTGG 4 cut(s) 878, 910, 1530, 1562
Alw21I GWGCWC 2 cut(s) 589, 1192
Alw26I GTCTC 3 cut(s) 208, 320, 2147
AlwI GGATC 5 cut(s) 614, 1175, 2315, 2460, 2498
Ama87I CYCGRG 2 cut(s) 2144, 2298
AoxI GGCC 5 cut(s) 506, 1052, 2594, 2619, 2746
ApaI GGGCCC 1 cut(s) 2750
ApeKI GCWGC 6 cut(s) 566, 1097, 1151, 1714, 1815, 2132
ApoI RAATTY 6 cut(s) 341, 577, 633, 887, 1494, 1599
Asp700I GAANNNNTTC 1 cut(s) 1580
Asp718I GGTACC 2 cut(s) 609, 1423
AspLEI GCGC 2 cut(s) 467, 872
AspS9I GGNCC 4 cut(s) 1032, 1052, 2746, 2747
AsuC2I CCSGG 3 cut(s) 293, 614, 2186
AsuHPI GGTGA 6 cut(s) 586, 1190, 1222, 1289, 1718, 2667
AsuNHI GCTAGC 1 cut(s) 1439
AvaI CYCGRG 2 cut(s) 2144, 2298
AvaII GGWCC 1 cut(s) 1032
BaeGI GKGCMC 1 cut(s) 2750
BaeI ACNNNNGTAYC 2 cut(s) 2158, 2191
BanI GGYRCC 3 cut(s) 609, 682, 1423
BanII GRGCYC 1 cut(s) 2750
BbsI GAAGAC 2 cut(s) 333, 2433
Bbv12I GWGCWC 2 cut(s) 589, 1192
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 6 cut(s) 578, 1084, 1138, 1701, 1827, 2144
BccI CCATC 4 cut(s) 806, 1871, 1897, 2785
BceAI ACGGC 2 cut(s) 325, 2606
BcgI CGANNNNNNTGC 2 cut(s) 2220, 2254
BciT130I CCWGG 7 cut(s) 62, 161, 176, 687, 822, 1436, 2710
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 3 cut(s) 293, 614, 2186
BcoDI GTCTC 3 cut(s) 208, 320, 2147
BfaI CTAG 7 cut(s) 570, 1440, 1812, 2172, 2256, 2337, 2513
BfmI CTRYAG 3 cut(s) 796, 964, 1304
BfuAI ACCTGC 1 cut(s) 1311
BisI GCNGC 7 cut(s) 567, 1055, 1098, 1152, 1715, 1816, 2133
BlpI GCTNAGC 1 cut(s) 791
BlsI GCNGC 7 cut(s) 568, 1056, 1099, 1153, 1716, 1817, 2134
BmcAI AGTACT 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 1032
BmeT110I CYCGRG 2 cut(s) 2144, 2298
BmgBI CACGTC 2 cut(s) 145, 2191
BmgT120I GGNCC 4 cut(s) 1032, 1052, 2746, 2747
BmiI GGNNCC 7 cut(s) 611, 684, 1034, 1425, 1986, 2053, 2748
BmrI ACTGGG 2 cut(s) 483, 2467
BmsI GCATC 8 cut(s) 349, 382, 589, 1402, 2119, 2290, 2505, 2620
BmtI GCTAGC 1 cut(s) 1443
BmuI ACTGGG 2 cut(s) 483, 2467
BoxI GACNNNNGTC 1 cut(s) 154
BpiI GAAGAC 2 cut(s) 333, 2433
BpmI CTGGAG 3 cut(s) 1022, 1418, 2692
Bpu10I CCTNAGC 1 cut(s) 503
Bpu1102I GCTNAGC 1 cut(s) 791
BpuMI CCSGG 3 cut(s) 293, 614, 2186
BsaAI YACGTR 2 cut(s) 1556, 1964
BsaBI GATNNNNATC 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 2147
BsaJI CCNNGG 9 cut(s) 60, 159, 160, 531, 765, 820, 1199, 1287, 2760
BsaWI WCCGGW 2 cut(s) 882, 1346
Bsc4I CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1029, 1739, 1994
Bse3DI GCAATG 2 cut(s) 1074, 2118
Bse8I GATNNNNATC 1 cut(s) 645
BseBI CCWGG 7 cut(s) 62, 161, 176, 687, 822, 1436, 2710
BseDI CCNNGG 9 cut(s) 60, 159, 160, 531, 765, 820, 1199, 1287, 2760
BseGI GGATG 8 cut(s) 364, 550, 622, 721, 817, 1768, 2178, 2278
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1029, 1739, 1994
BseMI GCAATG 2 cut(s) 1074, 2118
BseMII CTCAG 7 cut(s) 494, 782, 936, 1935, 2316, 2343, 2430
BseRI GAGGAG 2 cut(s) 289, 292
BseSI GKGCMC 1 cut(s) 2750
BseXI GCAGC 6 cut(s) 578, 1084, 1138, 1701, 1827, 2144
BseYI CCCAGC 1 cut(s) 2678
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 3 cut(s) 767, 1048, 2061
BshFI GGCC 5 cut(s) 508, 1054, 2596, 2621, 2748
BshNI GGYRCC 3 cut(s) 609, 682, 1423
BsiHKAI GWGCWC 2 cut(s) 589, 1192
BsiHKCI CYCGRG 2 cut(s) 2144, 2298
BsiSI CCGG 6 cut(s) 292, 614, 883, 1347, 1499, 2185
BslFI GGGAC 7 cut(s) 109, 142, 162, 974, 1657, 2201, 2759
BslI CCNNNNNNNGG 6 cut(s) 369, 826, 827, 1029, 1739, 1994
BsmAI GTCTC 3 cut(s) 208, 320, 2147
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 7 cut(s) 109, 142, 162, 974, 1657, 2201, 2759
BsmI GAATGC 3 cut(s) 955, 2022, 2607
BsnI GGCC 5 cut(s) 508, 1054, 2596, 2621, 2748
Bso31I GGTCTC 1 cut(s) 2147
BsoBI CYCGRG 2 cut(s) 2144, 2298
Bsp120I GGGCCC 1 cut(s) 2746
Bsp1286I GDGCHC 3 cut(s) 589, 1192, 2750
Bsp1407I TGTACA 1 cut(s) 1464
Bsp143I GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
Bsp1720I GCTNAGC 1 cut(s) 791
BspACI CCGC 6 cut(s) 743, 765, 767, 851, 1055, 1124
BspANI GGCC 5 cut(s) 508, 1054, 2596, 2621, 2748
BspCNI CTCAG 7 cut(s) 495, 783, 937, 1936, 2317, 2344, 2431
BspFNI CGCG 3 cut(s) 767, 1048, 2061
BspLI GGNNCC 7 cut(s) 611, 684, 1034, 1425, 1986, 2053, 2748
BspMAI CTGCAG 1 cut(s) 1308
BspMI ACCTGC 1 cut(s) 1311
BspOI GCTAGC 1 cut(s) 1443
BspPI GGATC 5 cut(s) 614, 1175, 2315, 2460, 2498
BspT107I GGYRCC 3 cut(s) 609, 682, 1423
BspTNI GGTCTC 1 cut(s) 2147
BsrDI GCAATG 2 cut(s) 1074, 2118
BsrGI TGTACA 1 cut(s) 1464
BssECI CCNNGG 9 cut(s) 60, 159, 160, 531, 765, 820, 1199, 1287, 2760
BssMI GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
Bst2UI CCWGG 7 cut(s) 62, 161, 176, 687, 822, 1436, 2710
Bst4CI ACNGT 6 cut(s) 268, 1464, 1509, 1624, 2075, 2308
Bst6I CTCTTC 2 cut(s) 1794, 2262
BstAPI GCANNNNNTGC 2 cut(s) 1776, 2129
BstAUI TGTACA 1 cut(s) 1464
BstBAI YACGTR 2 cut(s) 1556, 1964
BstC8I GCNNGC 6 cut(s) 1441, 1445, 1719, 1723, 1777, 2262
BstDEI CTNAG 9 cut(s) 503, 791, 945, 1944, 2095, 2212, 2325, 2352, 2439
BstDSI CCRYGG 4 cut(s) 531, 765, 1199, 1287
BstENI CCTNNNNNAGG 1 cut(s) 1027
BstF5I GGATG 8 cut(s) 364, 550, 622, 721, 817, 1768, 2178, 2278
BstFNI CGCG 3 cut(s) 767, 1048, 2061
BstHHI GCGC 2 cut(s) 467, 872
BstKTI GATC 9 cut(s) 423, 622, 649, 1183, 1798, 2065, 2323, 2468, 2506
BstMAI GTCTC 3 cut(s) 208, 320, 2147
BstMBI GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
BstMWI GCNNNNNNNGC 9 cut(s) 848, 950, 976, 1054, 1449, 1776, 2129, 2558, 2602
BstNI CCWGG 7 cut(s) 62, 161, 176, 687, 822, 1436, 2710
BstPAI GACNNNNGTC 1 cut(s) 154
BstSFI CTRYAG 3 cut(s) 796, 964, 1304
BstSLI GKGCMC 1 cut(s) 2750
BstSNI TACGTA 1 cut(s) 1556
BstUI CGCG 3 cut(s) 767, 1048, 2061
BstV1I GCAGC 6 cut(s) 578, 1084, 1138, 1701, 1827, 2144
BstV2I GAAGAC 2 cut(s) 333, 2433
BstX2I RGATCY 2 cut(s) 2465, 2503
BstXI CCANNNNNNTGG 1 cut(s) 1200
BstYI RGATCY 2 cut(s) 2465, 2503
BsuRI GGCC 5 cut(s) 508, 1054, 2596, 2621, 2748
BtgI CCRYGG 4 cut(s) 531, 765, 1199, 1287
BtrI CACGTC 2 cut(s) 145, 2191
BtsCI GGATG 8 cut(s) 364, 550, 622, 721, 817, 1768, 2178, 2278
BtsI GCAGTG 3 cut(s) 374, 936, 2659
BveI ACCTGC 1 cut(s) 1311
Cac8I GCNNGC 6 cut(s) 1441, 1445, 1719, 1723, 1777, 2262
CfoI GCGC 2 cut(s) 467, 872
Cfr13I GGNCC 4 cut(s) 1032, 1052, 2746, 2747
Cfr42I CCGCGG 1 cut(s) 768
CseI GACGC 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 685
Csp6I GTAC 8 cut(s) 247, 307, 610, 690, 705, 739, 1424, 1465
CspCI CAANNNNNGTGG 2 cut(s) 1396, 1431
CviAII CATG 8 cut(s) 132, 154, 988, 1147, 1924, 2224, 2539, 2582
CviQI GTAC 8 cut(s) 247, 307, 610, 690, 705, 739, 1424, 1465
DdeI CTNAG 9 cut(s) 503, 791, 945, 1944, 2095, 2212, 2325, 2352, 2439
DpnI GATC 9 cut(s) 422, 621, 648, 1182, 1797, 2064, 2322, 2467, 2505
DpnII GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
DraI TTTAAA 2 cut(s) 1383, 1531
DraIII CACNNNGTG 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 2619
Eam1104I CTCTTC 2 cut(s) 1794, 2262
EarI CTCTTC 2 cut(s) 1794, 2262
Eco105I TACGTA 1 cut(s) 1556
Eco147I AGGCCT 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 2750
Eco31I GGTCTC 1 cut(s) 2147
Eco47I GGWCC 1 cut(s) 1032
Eco57I CTGAAG 1 cut(s) 2574
Eco88I CYCGRG 2 cut(s) 2144, 2298
EcoNI CCTNNNNNAGG 1 cut(s) 1027
EcoO109I RGGNCCY 3 cut(s) 1032, 2746, 2747
EcoRII CCWGG 7 cut(s) 60, 159, 174, 685, 820, 1434, 2708
EcoT22I ATGCAT 1 cut(s) 2024
EcoT38I GRGCYC 1 cut(s) 2750
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 8 cut(s) 135, 157, 991, 1150, 1927, 2227, 2542, 2585
FaqI GGGAC 7 cut(s) 109, 142, 162, 974, 1657, 2201, 2759
FatI CATG 8 cut(s) 131, 153, 987, 1146, 1923, 2223, 2538, 2581
FauI CCCGC 1 cut(s) 750
FauNDI CATATG 2 cut(s) 406, 625
FbaI TGATCA 1 cut(s) 420
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 7 cut(s) 567, 1055, 1098, 1152, 1715, 1816, 2133
FokI GGATG 8 cut(s) 371, 557, 629, 728, 824, 1775, 2165, 2285
FriOI GRGCYC 1 cut(s) 2750
Fsp4HI GCNGC 7 cut(s) 567, 1055, 1098, 1152, 1715, 1816, 2133
FspBI CTAG 7 cut(s) 570, 1440, 1812, 2172, 2256, 2337, 2513
FspI TGCGCA 1 cut(s) 466
GlaI GCGC 2 cut(s) 466, 871
GluI GCNGC 7 cut(s) 567, 1055, 1098, 1152, 1715, 1816, 2133
GsaI CCCAGC 1 cut(s) 2682
GsuI CTGGAG 3 cut(s) 1022, 1418, 2692
HaeIII GGCC 5 cut(s) 508, 1054, 2596, 2621, 2748
HapII CCGG 6 cut(s) 292, 614, 883, 1347, 1499, 2185
HgaI GACGC 1 cut(s) 225
HhaI GCGC 2 cut(s) 467, 872
Hin1II CATG 8 cut(s) 135, 157, 991, 1150, 1927, 2227, 2542, 2585
Hin6I GCGC 2 cut(s) 465, 870
HinP1I GCGC 2 cut(s) 465, 870
HindIII AAGCTT 2 cut(s) 1450, 1843
HpaII CCGG 6 cut(s) 292, 614, 883, 1347, 1499, 2185
HphI GGTGA 6 cut(s) 586, 1190, 1222, 1289, 1718, 2667
Hpy166II GTNNAC 3 cut(s) 721, 2032, 2455
Hpy8I GTNNAC 3 cut(s) 721, 2032, 2455
Hpy99I CGWCG 1 cut(s) 439
HpyAV CCTTC 7 cut(s) 22, 1169, 1267, 1437, 1743, 1760, 2123
HpyCH4III ACNGT 6 cut(s) 268, 1464, 1509, 1624, 2075, 2308
HpyCH4IV ACGT 4 cut(s) 144, 1555, 1963, 2190
HpyF10VI GCNNNNNNNGC 9 cut(s) 848, 950, 976, 1054, 1449, 1776, 2129, 2558, 2602
HpyF3I CTNAG 9 cut(s) 503, 791, 945, 1944, 2095, 2212, 2325, 2352, 2439
HpySE526I ACGT 4 cut(s) 144, 1555, 1963, 2190
Hsp92II CATG 8 cut(s) 135, 157, 991, 1150, 1927, 2227, 2542, 2585
HspAI GCGC 2 cut(s) 465, 870
KpnI GGTACC 2 cut(s) 613, 1427
Ksp22I TGATCA 1 cut(s) 420
KspI CCGCGG 1 cut(s) 768
Kzo9I GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
LmnI GCTCC 7 cut(s) 499, 592, 898, 1195, 1226, 1291, 2392
Lsp1109I GCAGC 6 cut(s) 578, 1084, 1138, 1701, 1827, 2144
LweI GCATC 8 cut(s) 349, 382, 589, 1402, 2119, 2290, 2505, 2620
MabI ACCWGGT 1 cut(s) 685
MaeI CTAG 7 cut(s) 570, 1440, 1812, 2172, 2256, 2337, 2513
MaeII ACGT 4 cut(s) 144, 1555, 1963, 2190
MaeIII GTNAC 7 cut(s) 101, 379, 1334, 1706, 1939, 1959, 2275
MalI GATC 9 cut(s) 422, 621, 648, 1182, 1797, 2064, 2322, 2467, 2505
MboI GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
MflI RGATCY 2 cut(s) 2465, 2503
MhlI GDGCHC 3 cut(s) 589, 1192, 2750
MlyI GAGTC 5 cut(s) 144, 1006, 1639, 1703, 2543
MmeI TCCRAC 7 cut(s) 417, 655, 876, 1217, 1588, 2220, 2685
Mph1103I ATGCAT 1 cut(s) 2024
MroXI GAANNNNTTC 1 cut(s) 1580
MseI TTAA 9 cut(s) 750, 803, 1296, 1382, 1511, 1530, 1896, 2348, 2478
MslI CAYNNNNRTG 2 cut(s) 992, 2222
MspA1I CMGCKG 1 cut(s) 767
MspI CCGG 6 cut(s) 292, 614, 883, 1347, 1499, 2185
Mva1269I GAATGC 3 cut(s) 955, 2022, 2607
MvaI CCWGG 7 cut(s) 62, 161, 176, 687, 822, 1436, 2710
MvnI CGCG 3 cut(s) 767, 1048, 2061
MwoI GCNNNNNNNGC 9 cut(s) 848, 950, 976, 1054, 1449, 1776, 2129, 2558, 2602
NciI CCSGG 3 cut(s) 293, 614, 2186
NdeI CATATG 2 cut(s) 406, 625
NdeII GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
NheI GCTAGC 1 cut(s) 1439
NlaIII CATG 8 cut(s) 135, 157, 991, 1150, 1927, 2227, 2542, 2585
NlaIV GGNNCC 7 cut(s) 611, 684, 1034, 1425, 1986, 2053, 2748
NmeAIII GCCGAG 2 cut(s) 890, 1005
NmuCI GTSAC 3 cut(s) 379, 1706, 1959
NsbI TGCGCA 1 cut(s) 466
NsiI ATGCAT 1 cut(s) 2024
PaeR7I CTCGAG 1 cut(s) 2144
PasI CCCWGGG 1 cut(s) 160
PceI AGGCCT 1 cut(s) 508
PctI GAATGC 3 cut(s) 955, 2022, 2607
PdmI GAANNNNTTC 1 cut(s) 1580
PflMI CCANNNNNTGG 1 cut(s) 369
PfoI TCCNGGA 1 cut(s) 2708
PkrI GCNGC 7 cut(s) 568, 1056, 1099, 1153, 1716, 1817, 2134
PleI GAGTC 5 cut(s) 144, 1005, 1639, 1703, 2542
PpsI GAGTC 5 cut(s) 144, 1005, 1639, 1703, 2542
Ppu21I YACGTR 2 cut(s) 1556, 1964
PpuMI RGGWCCY 1 cut(s) 1032
PshAI GACNNNNGTC 1 cut(s) 154
Psp5II RGGWCCY 1 cut(s) 1032
Psp6I CCWGG 7 cut(s) 60, 159, 174, 685, 820, 1434, 2708
PspFI CCCAGC 1 cut(s) 2678
PspGI CCWGG 7 cut(s) 60, 159, 174, 685, 820, 1434, 2708
PspN4I GGNNCC 7 cut(s) 611, 684, 1034, 1425, 1986, 2053, 2748
PspOMI GGGCCC 1 cut(s) 2746
PspPI GGNCC 4 cut(s) 1032, 1052, 2746, 2747
PspPPI RGGWCCY 1 cut(s) 1032
PsrI GAACNNNNNNTAC 2 cut(s) 1452, 1484
PstI CTGCAG 1 cut(s) 1308
PsuI RGATCY 2 cut(s) 2465, 2503
RsaI GTAC 8 cut(s) 248, 308, 611, 691, 706, 740, 1425, 1466
RsaNI GTAC 8 cut(s) 247, 307, 610, 690, 705, 739, 1424, 1465
RseI CAYNNNNRTG 2 cut(s) 992, 2222
SacII CCGCGG 1 cut(s) 768
SaqAI TTAA 9 cut(s) 750, 803, 1296, 1382, 1511, 1530, 1896, 2348, 2478
SatI GCNGC 7 cut(s) 567, 1055, 1098, 1152, 1715, 1816, 2133
Sau3AI GATC 9 cut(s) 420, 619, 646, 1180, 1795, 2062, 2320, 2465, 2503
Sau96I GGNCC 4 cut(s) 1032, 1052, 2746, 2747
SbfI CCTGCAGG 1 cut(s) 1308
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 5 cut(s) 144, 1006, 1639, 1703, 2543
SdaI CCTGCAGG 1 cut(s) 1308
SduI GDGCHC 3 cut(s) 589, 1192, 2750
SexAI ACCWGGT 1 cut(s) 685
SfaNI GCATC 8 cut(s) 349, 382, 589, 1402, 2119, 2290, 2505, 2620
SfcI CTRYAG 3 cut(s) 796, 964, 1304
Sfr274I CTCGAG 1 cut(s) 2144
Sfr303I CCGCGG 1 cut(s) 768
SgrBI CCGCGG 1 cut(s) 768
SinI GGWCC 1 cut(s) 1032
SlaI CTCGAG 1 cut(s) 2144
SmiI ATTTAAAT 1 cut(s) 1531
SmiMI CAYNNNNRTG 2 cut(s) 992, 2222
SmlI CTYRAG 1 cut(s) 2144
SmoI CTYRAG 1 cut(s) 2144
SnaBI TACGTA 1 cut(s) 1556
Sse8387I CCTGCAGG 1 cut(s) 1308
SseBI AGGCCT 1 cut(s) 508
SsiI CCGC 6 cut(s) 743, 765, 767, 851, 1055, 1124
SspI AATATT 1 cut(s) 1546
SspMI CTAG 7 cut(s) 570, 1440, 1812, 2172, 2256, 2337, 2513
StuI AGGCCT 1 cut(s) 508
SwaI ATTTAAAT 1 cut(s) 1531
TaaI ACNGT 6 cut(s) 268, 1464, 1509, 1624, 2075, 2308
TaiI ACGT 4 cut(s) 147, 1558, 1966, 2193
TaqI TCGA 8 cut(s) 180, 345, 447, 491, 521, 832, 2145, 2591
TaqII GACCGA 1 cut(s) 1121
TatI WGTACW 3 cut(s) 246, 704, 1464
TauI GCSGC 1 cut(s) 1057
Tru1I TTAA 9 cut(s) 750, 803, 1296, 1382, 1511, 1530, 1896, 2348, 2478
Tru9I TTAA 9 cut(s) 750, 803, 1296, 1382, 1511, 1530, 1896, 2348, 2478
TseFI GTSAC 3 cut(s) 379, 1706, 1959
TseI GCWGC 6 cut(s) 566, 1097, 1151, 1714, 1815, 2132
Tsp45I GTSAC 3 cut(s) 379, 1706, 1959
TspGWI ACGGA 3 cut(s) 471, 548, 1188
Van91I CCANNNNNTGG 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 1032
XagI CCTNNNNNAGG 1 cut(s) 1027
XapI RAATTY 6 cut(s) 341, 577, 633, 887, 1494, 1599
XhoI CTCGAG 1 cut(s) 2144
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 1580
XspI CTAG 7 cut(s) 570, 1440, 1812, 2172, 2256, 2337, 2513
ZrmI AGTACT 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 2024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.