Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0010217

Physical Location & Seq

Genomic Coordinates Forward (+)

8328934 .. 8336667

Gene Structure 7734 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1117400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2514 bp

ATGGCCGGAACTGGAAGAAGCTCTCGGAGCAGAGTCAGCTCCTCCAGAGACGGCATTGACGGAGACGCCGTTCCTCTCCGCCCAACTCCCAATTTTGGGCCGCAGAGAGGTAGCAGAGGCAGAGGCAGAGGCAGAAATCAAACTTCAATCGGAACTTTCAACGTCAATCTCAAAGTCCTGCTAGGGCTCAGCGCGATCGCCTTCTTCGCCGTCTTGTATTTGATCATCAACCTCATGACCCATGCCGAACAAGCTCAGACGCCGAGGGTCGTCACGCCCTTCCCGGCTCCAAAGCTCATGGACCTCCCTCAGTTTCAAGGTGAGCACAAGGAGAGCTTGTATTGGGGAACATATCGCCCGAACGTTTATCTCGGAATCCGGGCTAGGACTCCCCGGTCTTTGATAGCCGGGTTAATGTGGATTGGTATAAAGGATGGGAGGTATTTCATGCGGCATGTTTGCCAAGATTCTGATGATCTGAGTAAATATGGTTGGGCACAACATGATGGGCGTAGTTTTGGACATCAAGTCATTGTTGACCAGGACATGAACTTGGGAACAAGTTTCTTGAAATCCAAGGGTGAAGACAGGAATGAGGAAGCTTCAAGAAGTGCACATCTCTTTTTCTATTTGGCCGATGAAGATGGAAATACTCTAAATTTAGGTGGAGATACCTTGGACATTCATGAGAACCCTCTCCTTGCATCTGGTTCACGGATGGACATTGGAAGTTGGCAGCTACATTTAAAATCACTGGACGATTTGGAAGTTCATTATGCTGGTTTTGAGACGCCTCATATTCACAACTTATCAGATCTTGTTCAGGAGAATCTTGGACTACAAGTAAGGAAGTTTGGTCGACTGCAGCTACCTGATACATCTGTTGACTCTCCAAATATTCTAGTTTTTCAGATTTCTGCTAGGGCTCCTTTCAGGACAGATGTTGCATTCATATCTGGAACTCGTTTGGAAAGTTCAAGAGTAGAAGAACGTGTCAGCAGCCTTACAGGTACCTCACTGACCAATCAACTGAAGGACAAGCAAAGAGAATTTGACACAAAATTTGAGAAGAGCTTTAATCTGGTTGACAAGCTTGATTCTGAATCTATTGTTGCTGGTAAGGCCGCTATAGCAAACTTGTTGGGTGGCATTGGCTACTTCTTTGGCCAGTCAAAAATTGCCGTCTCTGGGGATATGTTACCGGGAAGTCATGATAATTCTATCTTATATTGGCCTGCTGAGCTTTACACAGCTGTTCCCAGTCGACCATTTTTTCCAAGGGGATTTTTGTGGGATGAAGGTTTTCATCAACTTTTGATCTGGCGTTGGGATACACATATTTCCTTGGACATCATAGGACACTGGTTAGATCTGATGAATATTGATGGATGGATTCCACGTGAGCAAATTTTAGGAGTTGAAGCTCTAAGTAAAGTCCCAAGTGATTTTGTTACTCAGCTTCCAACAAATGGAAATCCACCAGCTCTGCTTTTGGCGTTGCGCGACCTAGTTCACGGCTTAAAGAAAAACAAGTTTACTGCTGCTGAAAGTGATGCGATATCTTCATTCGTAGAACGGGCTTTTGTTCGTCTAGAAGCATGGTTTCAATGGTTCAACACTACCCAGTCAGGGAAGGAAGTGGGCAGCTACTATTGGCATGGGAGAGACAACATAACAAATAGGGAACTCAACCCGAAGACGCTGACTTCTGGATTGGATGATTATCCACGTGCTTCCCATCCTAGCAAGGATGAGCGTCACGTAGATCTTAGATGCTGGATGCTACTTGCAGCAGATTGCTTGCATTCCATTGCGGAACTGTTTGAGAAGGAAAATAAGTCTGCGAAGGAATACGGTGCTACGGTTAAGTTACTCTCGGATTTTGAAGTACTGAATCAGATGCACTATGACGATGCATATGGAACATATCTTGATTTCGGAAATCATACGGAAAAGGTTCGTTTAGTTTGGAAAGAAACGATGACAGGACACAATTATGCAACTCGAGAGCTTGTACGGGAGGTATTGGAAAGCCCCAAGCTGAGACTGGTTCCTCACATTGGCCCTGTTAGTCTATTCCCCTTCATTGGAAGGATTATACCGGCTGATTCAAAGGTATTGGAAAAACAGCTAGACATTATATCAAATCGGAGTATCTTATGGACAGATTTTGGACTCCGCTCTCTTGCCAAAACAAGTTCCTTGTACATGAAATACAACACAGAGCATGATGCGCCTTACTGGAGGGGTCCAATATGGATGAACATGAATTACATGATTCTTTCGTCGCTCTATCATTACTCTCGAGTGGATGGACCGTACAGAGAAAAAGCGAGAACTATCTATGATGACTTAAGAGGCAATTTGATTAGAAACGTGGTGAAAAATTATCACAAAACCGGGTTCTTCTGGGAACAATATGATCAGAAGAACGGCAAGGGAAAAGGCGCACGCGTATTCAATGGCTGGACATCTCTCGTGTTGTTAATCATGGCGGAAGCTTACTCTAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004573 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006457 GO:0006464 GO:0006486 GO:0006487 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0015926 GO:0016020 GO:0016787 GO:0016798 GO:0019538 GO:0031984 GO:0034645 GO:0036211 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0070085 GO:0071704 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

Glyco_hydro_63 Glyco_hydro_63N

Protein Analysis

838

Amino Acids

94.95

Weight (kDa)

6.99

Isoelectric Point (pI)

37.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_63N	PF16923	112 - 252	6.1e-33	Glycosyl hydrolase family 63 N-terminal domain
Glyco_hydro_63	PF03200	336 - 833	1.9e-219	Glycosyl hydrolase family 63 C-terminal domain
MGH1-like_GH	PF22422	508 - 821	3.4e-18	Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67490 AT1G67490
fragaria_vesca	FvH4_4g23960 FvH4_4g23960
malus_domestica	MD13G1116700.v1.1 MD16G1117400.v1.1
prunus_persica	Prupe.1G228800_v2.0.a1
pyrus_communis	pycom13g10130 pycom16g10060
rosa_chinensis	RchiOBHm_Chr4g0430631
rosa_laevigata	RLG00000006961
rosa_multiflora	Rmu_sc0000131.1_g000015 Rmu_sc0005117.1_g000005 Rmu_sc0006863.1_g000002
rosa_roxburghii	Rroxscaffold_5G00372380
rosa_rugosa	Rorug04G0244900
rosa_samantha	Rh4AG300900 Rh4BG308000 Rh4CG325100 Rh4DG305600
rosa_wichuraiana	Rw4G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	394
Acc65I	GGTACC	1 cut(s)	1010
AccB1I	GGYRCC	1 cut(s)	1010
AccB7I	CCANNNNNTGG	1 cut(s)	1469
AccBSI	CCGCTC	1 cut(s)	2181
AccI	GTMKAC	2 cut(s)	859, 1264
AccII	CGCG	3 cut(s)	194, 1503, 2454
AciI	CCGC	7 cut(s)	79, 101, 451, 1125, 1814, 2179, 2495
AclI	AACGTT	1 cut(s)	363
AcoI	YGGCCR	3 cut(s)	3, 633, 1165
AcsI	RAATTY	4 cut(s)	658, 1049, 1061, 1407
AcuI	CTGAAG	1 cut(s)	1052
AcvI	CACGTG	2 cut(s)	1400, 1730
AcyI	GRCGYC	3 cut(s)	66, 260, 791
AfaI	GTAC	5 cut(s)	1012, 1890, 2016, 2207, 2321
AfiI	CCNNNNNNNGG	8 cut(s)	95, 96, 107, 1188, 1469, 1629, 2060, 2087
AflII	CTTAAG	1 cut(s)	2353
AflIII	ACRYGT	2 cut(s)	991, 2452
AhdI	GACNNNNNGTC	1 cut(s)	527
AjnI	CCWGG	1 cut(s)	540
AloI	GAACNNNNNNTCC	2 cut(s)	54, 86
Alw21I	GWGCWC	2 cut(s)	327, 616
Alw26I	GTCTC	6 cut(s)	42, 57, 782, 1189, 1659, 2038
Alw44I	GTGCAC	1 cut(s)	612
Ama87I	CYCGRG	2 cut(s)	2004, 2304
AoxI	GGCC	7 cut(s)	3, 98, 633, 1122, 1165, 1232, 2062
ApaLI	GTGCAC	1 cut(s)	612
ApeKI	GCWGC	6 cut(s)	736, 865, 999, 1541, 1644, 1790
ApoI	RAATTY	4 cut(s)	658, 1049, 1061, 1407
ArsI	GACNNNNNNTTYG	2 cut(s)	1046, 1078
AsiSI	GCGATCGC	1 cut(s)	198
Asp700I	GAANNNNTTC	2 cut(s)	1302, 1956
Asp718I	GGTACC	1 cut(s)	1010
AspLEI	GCGC	4 cut(s)	194, 1503, 2236, 2450
AspS9I	GGNCC	5 cut(s)	98, 301, 2063, 2249, 2315
AsuC2I	CCSGG	6 cut(s)	284, 380, 394, 409, 1203, 2401
AsuHPI	GGTGA	3 cut(s)	332, 593, 2392
AvaI	CYCGRG	2 cut(s)	2004, 2304
AvaII	GGWCC	3 cut(s)	301, 2249, 2315
BaeGI	GKGCMC	2 cut(s)	499, 616
BaeI	ACNNNNGTAYC	2 cut(s)	867, 900
BalI	TGGCCA	1 cut(s)	1167
BanI	GGYRCC	1 cut(s)	1010
BanII	GRGCYC	2 cut(s)	189, 928
BauI	CACGAG	1 cut(s)	2477
BbrPI	CACGTG	2 cut(s)	1400, 1730
BbsI	GAAGAC	2 cut(s)	591, 1703
Bbv12I	GWGCWC	2 cut(s)	327, 616
BbvI	GCAGC	6 cut(s)	748, 877, 1011, 1528, 1656, 1802
BccI	CCATC	8 cut(s)	428, 500, 638, 712, 1379, 1383, 1746, 2306
BceAI	ACGGC	6 cut(s)	53, 67, 194, 1166, 1531, 2449
BciT130I	CCWGG	1 cut(s)	542
BciVI	GTATCC	1 cut(s)	1324
BclI	TGATCA	2 cut(s)	222, 2422
BcnI	CCSGG	6 cut(s)	284, 380, 394, 409, 1203, 2401
BcoDI	GTCTC	6 cut(s)	42, 57, 782, 1189, 1659, 2038
BfmI	CTRYAG	2 cut(s)	863, 1128
BfrI	CTTAAG	1 cut(s)	2353
BfuI	GTATCC	1 cut(s)	1324
BglII	AGATCT	3 cut(s)	814, 1369, 1765
BisI	GCNGC	9 cut(s)	101, 452, 737, 866, 1000, 1125, 1542, 1645, 1791
BlpI	GCTNAGC	2 cut(s)	188, 1239
BlsI	GCNGC	9 cut(s)	102, 453, 738, 867, 1001, 1126, 1543, 1646, 1792
BmcAI	AGTACT	1 cut(s)	1890
Bme1390I	CCNGG	7 cut(s)	284, 380, 394, 409, 542, 1203, 2401
Bme18I	GGWCC	3 cut(s)	301, 2249, 2315
BmeRI	GACNNNNNGTC	1 cut(s)	527
BmeT110I	CYCGRG	2 cut(s)	2004, 2304
BmgT120I	GGNCC	5 cut(s)	98, 301, 2063, 2249, 2315
BmiI	GGNNCC	5 cut(s)	288, 927, 1012, 2052, 2250
BmrFI	CCNGG	7 cut(s)	284, 380, 394, 409, 542, 1203, 2401
BmrI	ACTGGG	2 cut(s)	1254, 1618
BmsI	GCATC	7 cut(s)	713, 1543, 1763, 1770, 1890, 1903, 2221
BmuI	ACTGGG	2 cut(s)	1254, 1618
BpiI	GAAGAC	2 cut(s)	591, 1703
BpmI	CTGGAG	2 cut(s)	28, 2263
Bpu1102I	GCTNAGC	2 cut(s)	188, 1239
BpuMI	CCSGG	6 cut(s)	284, 380, 394, 409, 1203, 2401
BsaAI	YACGTR	3 cut(s)	1400, 1730, 1762
BsaBI	GATNNNNATC	1 cut(s)	1722
BsaHI	GRCGYC	3 cut(s)	66, 260, 791
BsaJI	CCNNGG	6 cut(s)	263, 392, 576, 675, 1277, 1344
BsaXI	ACNNNNNCTCC	4 cut(s)	54, 84, 323, 353
Bsc4I	CCNNNNNNNGG	8 cut(s)	95, 96, 107, 1188, 1469, 1629, 2060, 2087
Bse118I	RCCGGY	1 cut(s)	2101
Bse1I	ACTGG	8 cut(s)	16, 759, 1168, 1260, 1367, 1624, 2052, 2246
Bse3DI	GCAATG	1 cut(s)	1809
Bse8I	GATNNNNATC	1 cut(s)	1722
BseBI	CCWGG	1 cut(s)	542
BseDI	CCNNGG	6 cut(s)	263, 392, 576, 675, 1277, 1344
BseJI	GATNNNNATC	1 cut(s)	1722
BseLI	CCNNNNNNNGG	8 cut(s)	95, 96, 107, 1188, 1469, 1629, 2060, 2087
BseMI	GCAATG	1 cut(s)	1809
BseMII	CTCAG	7 cut(s)	202, 269, 323, 470, 1230, 1469, 2033
BseNI	ACTGG	8 cut(s)	16, 759, 1168, 1260, 1367, 1624, 2052, 2246
BseRI	GAGGAG	1 cut(s)	31
BseSI	GKGCMC	2 cut(s)	499, 616
BseXI	GCAGC	6 cut(s)	748, 877, 1011, 1528, 1656, 1802
Bsh1236I	CGCG	3 cut(s)	194, 1503, 2454
Bsh1285I	CGRYCG	1 cut(s)	198
BshFI	GGCC	7 cut(s)	5, 100, 635, 1124, 1167, 1234, 2064
BshNI	GGYRCC	1 cut(s)	1010
BsiEI	CGRYCG	1 cut(s)	198
BsiHKAI	GWGCWC	2 cut(s)	327, 616
BsiHKCI	CYCGRG	2 cut(s)	2004, 2304
BsiSI	CCGG	8 cut(s)	6, 284, 379, 394, 408, 1202, 2102, 2400
BslFI	GGGAC	1 cut(s)	1421
BslI	CCNNNNNNNGG	8 cut(s)	95, 96, 107, 1188, 1469, 1629, 2060, 2087
BsmAI	GTCTC	6 cut(s)	42, 57, 782, 1189, 1659, 2038
BsmBI	CGTCTC	4 cut(s)	42, 57, 782, 1189
BsmFI	GGGAC	1 cut(s)	1421
BsmI	GAATGC	2 cut(s)	947, 1804
BsnI	GGCC	7 cut(s)	5, 100, 635, 1124, 1167, 1234, 2064
BsoBI	CYCGRG	2 cut(s)	2004, 2304
Bsp1286I	GDGCHC	5 cut(s)	189, 327, 499, 616, 928
Bsp1407I	TGTACA	1 cut(s)	2205
Bsp143I	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
Bsp1720I	GCTNAGC	2 cut(s)	188, 1239
BspACI	CCGC	7 cut(s)	79, 101, 451, 1125, 1814, 2179, 2495
BspANI	GGCC	7 cut(s)	5, 100, 635, 1124, 1167, 1234, 2064
BspCNI	CTCAG	7 cut(s)	201, 268, 322, 471, 1231, 1468, 2034
BspFNI	CGCG	3 cut(s)	194, 1503, 2454
BspHI	TCATGA	3 cut(s)	234, 685, 1210
BspLI	GGNNCC	5 cut(s)	288, 927, 1012, 2052, 2250
BspMAI	CTGCAG	1 cut(s)	867
BspQI	GCTCTTC	1 cut(s)	1064
BspT107I	GGYRCC	1 cut(s)	1010
BspTI	CTTAAG	1 cut(s)	2353
BsrBI	CCGCTC	1 cut(s)	2181
BsrDI	GCAATG	1 cut(s)	1809
BsrFI	RCCGGY	1 cut(s)	2101
BsrGI	TGTACA	1 cut(s)	2205
BsrI	ACTGG	8 cut(s)	16, 759, 1168, 1260, 1367, 1624, 2052, 2246
BssAI	RCCGGY	1 cut(s)	2101
BssECI	CCNNGG	6 cut(s)	263, 392, 576, 675, 1277, 1344
BssMI	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
BssNI	GRCGYC	3 cut(s)	66, 260, 791
BssSI	CACGAG	1 cut(s)	2477
BssT1I	CCWWGG	4 cut(s)	576, 675, 1277, 1344
Bst2BI	CACGAG	1 cut(s)	2477
Bst2UI	CCWGG	1 cut(s)	542
Bst4CI	ACNGT	4 cut(s)	1821, 1856, 1864, 2319
Bst6I	CTCTTC	1 cut(s)	1064
BstACI	GRCGYC	3 cut(s)	66, 260, 791
BstAFI	CTTAAG	1 cut(s)	2353
BstAUI	TGTACA	1 cut(s)	2205
BstBAI	YACGTR	3 cut(s)	1400, 1730, 1762
BstC8I	GCNNGC	3 cut(s)	1236, 1802, 2452
BstDEI	CTNAG	9 cut(s)	188, 255, 309, 479, 1239, 1427, 1455, 1769, 2042
BstFNI	CGCG	3 cut(s)	194, 1503, 2454
BstHHI	GCGC	4 cut(s)	194, 1503, 2236, 2450
BstKTI	GATC	8 cut(s)	198, 225, 478, 817, 1320, 1372, 1768, 2425
BstMAI	GTCTC	6 cut(s)	42, 57, 782, 1189, 1659, 2038
BstMBI	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
BstMCI	CGRYCG	1 cut(s)	198
BstMWI	GCNNNNNNNGC	8 cut(s)	27, 36, 206, 251, 1121, 1130, 1240, 2233
BstNI	CCWGG	1 cut(s)	542
BstNSI	RCATGY	1 cut(s)	458
BstSCI	CCNGG	7 cut(s)	282, 378, 392, 407, 540, 1201, 2399
BstSFI	CTRYAG	2 cut(s)	863, 1128
BstSLI	GKGCMC	2 cut(s)	499, 616
BstUI	CGCG	3 cut(s)	194, 1503, 2454
BstV1I	GCAGC	6 cut(s)	748, 877, 1011, 1528, 1656, 1802
BstV2I	GAAGAC	2 cut(s)	591, 1703
BstX2I	RGATCY	3 cut(s)	814, 1369, 1765
BstYI	RGATCY	3 cut(s)	814, 1369, 1765
BsuI	GTATCC	1 cut(s)	1324
BsuRI	GGCC	7 cut(s)	5, 100, 635, 1124, 1167, 1234, 2064
BtsIMutI	CAGTG	3 cut(s)	752, 1016, 1360
Cac8I	GCNNGC	3 cut(s)	1236, 1802, 2452
CciI	TCATGA	3 cut(s)	234, 685, 1210
CfoI	GCGC	4 cut(s)	194, 1503, 2236, 2450
Cfr10I	RCCGGY	1 cut(s)	2101
Cfr13I	GGNCC	5 cut(s)	98, 301, 2063, 2249, 2315
CseI	GACGC	5 cut(s)	74, 268, 799, 1708, 1745
Csp6I	GTAC	5 cut(s)	1011, 1889, 2015, 2206, 2320
CviQI	GTAC	5 cut(s)	1011, 1889, 2015, 2206, 2320
DdeI	CTNAG	9 cut(s)	188, 255, 309, 479, 1239, 1427, 1455, 1769, 2042
DpnI	GATC	8 cut(s)	197, 224, 477, 816, 1319, 1371, 1767, 2424
DpnII	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
DraI	TTTAAA	1 cut(s)	747
DrdI	GACNNNNNNGTC	1 cut(s)	394
DriI	GACNNNNNGTC	1 cut(s)	527
DseDI	GACNNNNNNGTC	1 cut(s)	394
EaeI	YGGCCR	3 cut(s)	3, 633, 1165
Eam1104I	CTCTTC	1 cut(s)	1064
Eam1105I	GACNNNNNGTC	1 cut(s)	527
EarI	CTCTTC	1 cut(s)	1064
EciI	GGCGGA	2 cut(s)	68, 2510
Eco130I	CCWWGG	4 cut(s)	576, 675, 1277, 1344
Eco24I	GRGCYC	2 cut(s)	189, 928
Eco32I	GATATC	1 cut(s)	1560
Eco47I	GGWCC	3 cut(s)	301, 2249, 2315
Eco57I	CTGAAG	1 cut(s)	1052
Eco72I	CACGTG	2 cut(s)	1400, 1730
Eco88I	CYCGRG	2 cut(s)	2004, 2304
EcoRII	CCWGG	1 cut(s)	540
EcoRV	GATATC	1 cut(s)	1560
EcoT14I	CCWWGG	4 cut(s)	576, 675, 1277, 1344
EcoT22I	ATGCAT	1 cut(s)	1918
EcoT38I	GRGCYC	2 cut(s)	189, 928
ErhI	CCWWGG	4 cut(s)	576, 675, 1277, 1344
Esp3I	CGTCTC	4 cut(s)	42, 57, 782, 1189
FalI	AAGNNNNNCTT	2 cut(s)	320, 352
FaqI	GGGAC	1 cut(s)	1421
FauNDI	CATATG	1 cut(s)	1918
FbaI	TGATCA	2 cut(s)	222, 2422
FblI	GTMKAC	2 cut(s)	859, 1264
Fnu4HI	GCNGC	9 cut(s)	101, 452, 737, 866, 1000, 1125, 1542, 1645, 1791
FriOI	GRGCYC	2 cut(s)	189, 928
Fsp4HI	GCNGC	9 cut(s)	101, 452, 737, 866, 1000, 1125, 1542, 1645, 1791
GlaI	GCGC	4 cut(s)	193, 1502, 2235, 2449
GluI	GCNGC	9 cut(s)	101, 452, 737, 866, 1000, 1125, 1542, 1645, 1791
GsuI	CTGGAG	2 cut(s)	28, 2263
HaeIII	GGCC	7 cut(s)	5, 100, 635, 1124, 1167, 1234, 2064
HapII	CCGG	8 cut(s)	6, 284, 379, 394, 408, 1202, 2102, 2400
HgaI	GACGC	5 cut(s)	74, 268, 799, 1708, 1745
HhaI	GCGC	4 cut(s)	194, 1503, 2236, 2450
Hin1I	GRCGYC	3 cut(s)	66, 260, 791
Hin6I	GCGC	4 cut(s)	192, 1501, 2234, 2448
HinP1I	GCGC	4 cut(s)	192, 1501, 2234, 2448
HincII	GTYRAC	5 cut(s)	538, 860, 886, 1087, 1265
HindII	GTYRAC	5 cut(s)	538, 860, 886, 1087, 1265
HindIII	AAGCTT	3 cut(s)	600, 1091, 2499
HpaII	CCGG	8 cut(s)	6, 284, 379, 394, 408, 1202, 2102, 2400
HphI	GGTGA	3 cut(s)	332, 593, 2392
Hpy166II	GTNNAC	9 cut(s)	538, 614, 713, 860, 886, 1087, 1265, 1513, 1536
Hpy8I	GTNNAC	9 cut(s)	538, 614, 713, 860, 886, 1087, 1265, 1513, 1536
Hpy99I	CGWCG	1 cut(s)	2290
HpyAV	CCTTC	9 cut(s)	211, 289, 1027, 1292, 1627, 1822, 1840, 2085, 2092
HpyCH4III	ACNGT	4 cut(s)	1821, 1856, 1864, 2319
HpyCH4IV	ACGT	7 cut(s)	162, 363, 991, 1399, 1729, 1761, 2376
HpyCH4V	TGCA	9 cut(s)	614, 704, 865, 947, 1790, 1804, 1903, 1916, 2000
HpyF10VI	GCNNNNNNNGC	8 cut(s)	27, 36, 206, 251, 1121, 1130, 1240, 2233
HpyF3I	CTNAG	9 cut(s)	188, 255, 309, 479, 1239, 1427, 1455, 1769, 2042
HpySE526I	ACGT	7 cut(s)	162, 363, 991, 1399, 1729, 1761, 2376
Hsp92I	GRCGYC	3 cut(s)	66, 260, 791
HspAI	GCGC	4 cut(s)	192, 1501, 2234, 2448
KpnI	GGTACC	1 cut(s)	1014
Ksp22I	TGATCA	2 cut(s)	222, 2422
Kzo9I	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
LguI	GCTCTTC	1 cut(s)	1064
LmnI	GCTCC	4 cut(s)	27, 44, 292, 931
Lsp1109I	GCAGC	6 cut(s)	748, 877, 1011, 1528, 1656, 1802
LweI	GCATC	7 cut(s)	713, 1543, 1763, 1770, 1890, 1903, 2221
MaeII	ACGT	7 cut(s)	162, 363, 991, 1399, 1729, 1761, 2376
MaeIII	GTNAC	5 cut(s)	271, 1197, 1450, 1757, 1869
MalI	GATC	8 cut(s)	197, 224, 477, 816, 1319, 1371, 1767, 2424
MbiI	CCGCTC	1 cut(s)	2181
MboI	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
MflI	RGATCY	3 cut(s)	814, 1369, 1765
MhlI	GDGCHC	5 cut(s)	189, 327, 499, 616, 928
MlsI	TGGCCA	1 cut(s)	1167
MluI	ACGCGT	1 cut(s)	2452
MluNI	TGGCCA	1 cut(s)	1167
MlyI	GAGTC	4 cut(s)	42, 382, 881, 2169
MmeI	TCCRAC	1 cut(s)	1487
Mox20I	TGGCCA	1 cut(s)	1167
Mph1103I	ATGCAT	1 cut(s)	1918
MroXI	GAANNNNTTC	2 cut(s)	1302, 1956
MscI	TGGCCA	1 cut(s)	1167
MseI	TTAA	7 cut(s)	413, 746, 1077, 1520, 1866, 2354, 2486
MslI	CAYNNNNRTG	1 cut(s)	1995
Msp20I	TGGCCA	1 cut(s)	1167
MspA1I	CMGCKG	1 cut(s)	1253
MspCI	CTTAAG	1 cut(s)	2353
MspI	CCGG	8 cut(s)	6, 284, 379, 394, 408, 1202, 2102, 2400
MspR9I	CCNGG	7 cut(s)	284, 380, 394, 409, 542, 1203, 2401
Mva1269I	GAATGC	2 cut(s)	947, 1804
MvaI	CCWGG	1 cut(s)	542
MvnI	CGCG	3 cut(s)	194, 1503, 2454
MwoI	GCNNNNNNNGC	8 cut(s)	27, 36, 206, 251, 1121, 1130, 1240, 2233
NciI	CCSGG	6 cut(s)	284, 380, 394, 409, 1203, 2401
NdeI	CATATG	1 cut(s)	1918
NdeII	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
NlaIV	GGNNCC	5 cut(s)	288, 927, 1012, 2052, 2250
NmeAIII	GCCGAG	1 cut(s)	288
NmuCI	GTSAC	2 cut(s)	271, 1757
NsiI	ATGCAT	1 cut(s)	1918
NspI	RCATGY	1 cut(s)	458
PaeR7I	CTCGAG	2 cut(s)	2004, 2304
PagI	TCATGA	3 cut(s)	234, 685, 1210
PciSI	GCTCTTC	1 cut(s)	1064
PcsI	WCGNNNNNNNCGW	1 cut(s)	66
PctI	GAATGC	2 cut(s)	947, 1804
PdmI	GAANNNNTTC	2 cut(s)	1302, 1956
PfeI	GAWTC	9 cut(s)	375, 467, 829, 1097, 1103, 1393, 1894, 2108, 2278
PflMI	CCANNNNNTGG	1 cut(s)	1469
PkrI	GCNGC	9 cut(s)	102, 453, 738, 867, 1001, 1126, 1543, 1646, 1792
Ple19I	CGATCG	1 cut(s)	198
PleI	GAGTC	4 cut(s)	41, 382, 881, 2169
PmaCI	CACGTG	2 cut(s)	1400, 1730
PmlI	CACGTG	2 cut(s)	1400, 1730
PpsI	GAGTC	4 cut(s)	41, 382, 881, 2169
Ppu21I	YACGTR	3 cut(s)	1400, 1730, 1762
Psp1406I	AACGTT	1 cut(s)	363
Psp6I	CCWGG	1 cut(s)	540
PspCI	CACGTG	2 cut(s)	1400, 1730
PspGI	CCWGG	1 cut(s)	540
PspN4I	GGNNCC	5 cut(s)	288, 927, 1012, 2052, 2250
PspPI	GGNCC	5 cut(s)	98, 301, 2063, 2249, 2315
PsrI	GAACNNNNNNTAC	2 cut(s)	2255, 2287
PstI	CTGCAG	1 cut(s)	867
PsuI	RGATCY	3 cut(s)	814, 1369, 1765
PvuI	CGATCG	1 cut(s)	198
PvuII	CAGCTG	1 cut(s)	1253
RgaI	GCGATCGC	1 cut(s)	198
RsaI	GTAC	5 cut(s)	1012, 1890, 2016, 2207, 2321
RsaNI	GTAC	5 cut(s)	1011, 1889, 2015, 2206, 2320
RseI	CAYNNNNRTG	1 cut(s)	1995
SalI	GTCGAC	2 cut(s)	858, 1263
SapI	GCTCTTC	1 cut(s)	1064
SaqAI	TTAA	7 cut(s)	413, 746, 1077, 1520, 1866, 2354, 2486
SatI	GCNGC	9 cut(s)	101, 452, 737, 866, 1000, 1125, 1542, 1645, 1791
Sau3AI	GATC	8 cut(s)	195, 222, 475, 814, 1317, 1369, 1765, 2422
Sau96I	GGNCC	5 cut(s)	98, 301, 2063, 2249, 2315
ScaI	AGTACT	1 cut(s)	1890
SchI	GAGTC	4 cut(s)	42, 382, 881, 2169
ScrFI	CCNGG	7 cut(s)	284, 380, 394, 409, 542, 1203, 2401
SduI	GDGCHC	5 cut(s)	189, 327, 499, 616, 928
SfaAI	GCGATCGC	1 cut(s)	198
SfaNI	GCATC	7 cut(s)	713, 1543, 1763, 1770, 1890, 1903, 2221
SfcI	CTRYAG	2 cut(s)	863, 1128
Sfr274I	CTCGAG	2 cut(s)	2004, 2304
SgfI	GCGATCGC	1 cut(s)	198
SinI	GGWCC	3 cut(s)	301, 2249, 2315
SlaI	CTCGAG	2 cut(s)	2004, 2304
SmiMI	CAYNNNNRTG	1 cut(s)	1995
SmlI	CTYRAG	3 cut(s)	2004, 2304, 2353
SmoI	CTYRAG	3 cut(s)	2004, 2304, 2353
SsiI	CCGC	7 cut(s)	79, 101, 451, 1125, 1814, 2179, 2495
SspI	AATATT	2 cut(s)	898, 1381
StyD4I	CCNGG	7 cut(s)	282, 378, 392, 407, 540, 1201, 2399
StyI	CCWWGG	4 cut(s)	576, 675, 1277, 1344
TaaI	ACNGT	4 cut(s)	1821, 1856, 1864, 2319
TaiI	ACGT	7 cut(s)	165, 366, 994, 1402, 1732, 1764, 2379
TaqI	TCGA	4 cut(s)	859, 1264, 2005, 2305
TatI	WGTACW	2 cut(s)	1888, 2205
TauI	GCSGC	3 cut(s)	103, 454, 1127
TfiI	GAWTC	9 cut(s)	375, 467, 829, 1097, 1103, 1393, 1894, 2108, 2278
Tru1I	TTAA	7 cut(s)	413, 746, 1077, 1520, 1866, 2354, 2486
Tru9I	TTAA	7 cut(s)	413, 746, 1077, 1520, 1866, 2354, 2486
TscAI	CASTG	3 cut(s)	759, 1023, 1367
TseFI	GTSAC	2 cut(s)	271, 1757
TseI	GCWGC	6 cut(s)	736, 865, 999, 1541, 1644, 1790
Tsp45I	GTSAC	2 cut(s)	271, 1757
TspGWI	ACGGA	3 cut(s)	75, 730, 1964
TspRI	CASTG	3 cut(s)	759, 1023, 1367
Van91I	CCANNNNNTGG	1 cut(s)	1469
Vha464I	CTTAAG	1 cut(s)	2353
VneI	GTGCAC	1 cut(s)	612
VpaK11BI	GGWCC	3 cut(s)	301, 2249, 2315
XapI	RAATTY	4 cut(s)	658, 1049, 1061, 1407
XbaI	TCTAGA	1 cut(s)	1591
XceI	RCATGY	1 cut(s)	458
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2044
XhoI	CTCGAG	2 cut(s)	2004, 2304
XmiI	GTMKAC	2 cut(s)	859, 1264
XmnI	GAANNNNTTC	2 cut(s)	1302, 1956
ZrmI	AGTACT	1 cut(s)	1890
Zsp2I	ATGCAT	1 cut(s)	1918

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.