Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0010217

Physical Location & Seq

Genomic Coordinates Forward (+)

12174651 .. 12182005

Gene Structure 7355 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006961

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGTCTGGAACCGGAAAACGAAGCACTAGTAGTCGGAGCCGAGTCAAACCGTCCAAGCACTCCAACGACGACGAAGACGACGGCGTTTCTCTCCGCCGTCGTAGTAGGCCCAATCTCCGCCGCCGAAACGACAGCTCGATCAGAATCTTCAACGTCGATCTCAAAATCGTGCTAGGGCTCAGCGTCGTGGCCTTCTTTGTCATTTTTTTTTTCATCTATAACCTCATAAACCATGTCGCACAGCCTCAGATACCTAGGGTCGTCACGCCATTCCCTGCTCCGAAGCTCATGGACCTCCCTCAGTTTCAAGGTGAGCATAAGGAGAGCTTGTATTGGGGAACGTATCGGCCTCATGCTTATCTCGGAATCCGGGCTAGGACTCCTCGGTCTTTGATTGCTGGATTAATGTGGCTAGGAATACAGGACGGGAGATATTCCCTGCGGCATTTTTGCCAAGATTCTGATGGTCTGGCCCAATATGGTTGGACAGATCATGATGGGCGCACTTATGGACATCAAGTCATTGTTGACCAGGAAATGACGTTGGCCACTAGTTTCTTGAAATCGAAAGGGGAAGACAGTGGTTACGGGGGAGACTGGGCAGTTCGACTTGATGTACAAAACCACAAATCTAACGGCAATGATGAACTTTCGAGAAGTGCACATCTCTTTTTCTATTTGGCTGATGAGGAGGGAAATGCTCTAACTGTAGGAGAAGACACATTGGGAACTTATGAGAATTCCCTCCTTGCATCTGGTTCTCGTATGGACGTTGGAAGTTGGCAGCTGCATCTAAAATCAGAAGATGATTTGGAAGTTCATTATTCTGGTTTTAAGACGCCTCATATTCACAACTTATCTGATCTTGTCCAGGAAAATCTTGGACTACAAGTAAGAAAATTTAATCGACTGCAGCTACCTGACTCATTTGAAGATTATCCAAGTATTCTGGTTTTTCAGATTTCTGCTAAGATTCCTTTCAAGGCGGATATCACATTTGTATCTGGAACTGGTTTGGAAAGTTCAAGAGTAGAGAAACGTGTCAGCAGCCTTACAGGTACTTCACTAACCAGCCAACTGAAAGAGAAGCAAAGAGAATTTGACTCGAAGTTTGAGAAGACCTTTAACCTAACTGACAAGGTTGATCCTGAATCTATTATTGCTGGTAAGGCTGCTATTGGAAACTTGTTGGGTGGCATCGGCTATTTCTTTGGCCAGTCAAAAATTGCCCTGCCTAGGGATGTGAATCTGGCAAGTCAGGATAATTCTATTTTATATTGGCCTGCCGAGCTTTACACAGCTGTTCCAAGTCGACCTTTCTTTCCAAGGGGATTTTTGTGGGATGAAGGCTTTCATCAGCTCTTGATTTGGCGTTGGGATACACACATTTCTTTAGACATCTTAGGACACTGGTTAGATCTGATGAATATTGATGGATGGATTCCACGTGAGCAAATTTTAGGTGCCGAAGCTCTCAGTAAAGTCCCCAAGGAATTTGTTGCTCAGCTTCCATCAAATGGAAATCCACCAACTCTATTTTTAGTATTACGTGAACTACTTCATGGTTTAAAGAAAAATCAGTTTACTGCTCCTGAAAGCAATGAGATCTCTTCATTCCTAAAACAGGCTTTTGTTCGCCTAGAAGCATGGTTTCAGTGGTTCAACACTACCCAGTCAGGAAAAGAAATTGGCGCCTACTACTGGCATGGAAGAGACCATACAACAACGCGGCAACTAAATCCGCAGACACTGTCTTCTGGGTTTGATGATTATCCTCGTGCTTCTCACCCAAGTGAGGATGAACGTCATGTAGATCTTAGATGCTGGATGCTTCTTGCAGCAGATTGCTTGCATTCCATTTCGAAGCTTTTTGAGGAGGATAACAAATTTGGAAAGGAGTATGATACTACAGTTAAGATGCTCTCGGATTTTGAAATTCTGAATCAGATGCATTATGACGATGCGTATGGAGCTTACTTTGATTTCGGGAATCATACAGAAAAGGTTCGTTTAAGGTGGAAGGAAATGATGGTGGGACACAACTATCCAACTCGAGAGCTTTTACGGGAAGTATTGGAAACTCCCAAGCTGAGACTGGTTCCTCACATTGGCTATGTTAGTCTTTTCCCCTTCATGGGAAGGATTATTCCAGCTGATTCGTGGATTCTGGAGAAACAGCTAGATCTTATATCAAACCGGAGTATCTTGTGGACAGATTTTGGACTGCGTTCTCTTGCAAAAACAAGCTCCATCTATATGAAACATAACACAGAGCATGACGCTCCTTATTGGAGGGGTCCAATATGGATGAACATGAATTACATGATTCTTTCATCACTCTATCATTACTCCACAGAGGATGGGCCATATAGAGAAAAGGCAAGAACCATCTATGATGACTTGAGAGGCAATTTGATTAGAAATGTGGTTCGGAATTACCACAAAGCTGGGTTCTTCTGGGAACAGTTTGATCAGAGAAATGGCAAGGGTAAAGGTGCACGCGTATTTACTGGTTGGACATCCCTTGTCTTGTTAATCATGGCAGAAGCTTACCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004573 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006457 GO:0006464 GO:0006486 GO:0006487 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0012505 GO:0015926 GO:0016020 GO:0016787 GO:0016798 GO:0019538 GO:0031984 GO:0034645 GO:0036211 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0070085 GO:0071704 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

Glyco_hydro_63 Glyco_hydro_63N

Protein Analysis

853

Amino Acids

97.94

Weight (kDa)

6.6

Isoelectric Point (pI)

46.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_63N	PF16923	109 - 273	3.9e-42	Glycosyl hydrolase family 63 N-terminal domain
Glyco_hydro_63	PF03200	352 - 849	8.9e-221	Glycosyl hydrolase family 63 C-terminal domain
MGH1-like_GH	PF22422	536 - 837	1.2e-16	Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010217)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67490 AT1G67490
fragaria_vesca	FvH4_4g23960 FvH4_4g23960
malus_domestica	MD13G1116700.v1.1 MD16G1117400.v1.1
prunus_persica	Prupe.1G228800_v2.0.a1
pyrus_communis	pycom13g10130 pycom16g10060
rosa_chinensis	RchiOBHm_Chr4g0430631
rosa_laevigata	RLG00000006961
rosa_multiflora	Rmu_sc0000131.1_g000015 Rmu_sc0005117.1_g000005 Rmu_sc0006863.1_g000002
rosa_roxburghii	Rroxscaffold_5G00372380
rosa_rugosa	Rorug04G0244900
rosa_samantha	Rh4AG300900 Rh4BG308000 Rh4CG325100 Rh4DG305600
rosa_wichuraiana	Rw4G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	385
AccB1I	GGYRCC	2 cut(s)	1463, 1691
AccB7I	CCANNNNNTGG	1 cut(s)	1517
AccI	GTMKAC	1 cut(s)	1312
AccII	CGCG	2 cut(s)	1729, 2502
AciI	CCGC	7 cut(s)	94, 118, 121, 442, 986, 1729, 1742
AclWI	GGATC	1 cut(s)	1139
AcoI	YGGCCR	2 cut(s)	546, 1213
AcsI	RAATTY	7 cut(s)	739, 899, 1097, 1455, 1493, 1886, 1935
AcvI	CACGTG	1 cut(s)	1448
AcyI	GRCGYC	2 cut(s)	839, 1692
AfaI	GTAC	2 cut(s)	618, 1060
AfiI	CCNNNNNNNGG	9 cut(s)	1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
AflIII	ACRYGT	2 cut(s)	1039, 2500
AgsI	TTSAA	8 cut(s)	151, 308, 562, 932, 982, 1026, 1663, 1934
AhdI	GACNNNNNGTC	1 cut(s)	518
AhlI	ACTAGT	2 cut(s)	26, 551
AjnI	CCWGG	2 cut(s)	531, 870
AleI	CACNNNNGTG	1 cut(s)	1791
Alw21I	GWGCWC	2 cut(s)	664, 2500
Alw26I	GTCTC	3 cut(s)	588, 1707, 2086
Alw44I	GTGCAC	2 cut(s)	660, 2496
AlwI	GGATC	1 cut(s)	1139
AlwNI	CAGNNNCTG	1 cut(s)	1750
Ama87I	CYCGRG	1 cut(s)	2052
AoxI	GGCC	8 cut(s)	107, 189, 347, 471, 546, 1213, 1280, 2363
ApaLI	GTGCAC	2 cut(s)	660, 2496
ApeKI	GCWGC	6 cut(s)	784, 787, 913, 1047, 1172, 1838
ApoI	RAATTY	7 cut(s)	739, 899, 1097, 1455, 1493, 1886, 1935
ArsI	GACNNNNNNTTYG	2 cut(s)	1094, 1126
AseI	ATTAAT	1 cut(s)	404
Asp700I	GAANNNNTTC	3 cut(s)	1350, 1557, 2004
AspA2I	CCTAGG	2 cut(s)	254, 1235
AspLEI	GCGC	2 cut(s)	504, 1694
AspS9I	GGNCC	5 cut(s)	108, 292, 472, 2297, 2363
AsuC2I	CCSGG	1 cut(s)	371
AsuHPI	GGTGA	2 cut(s)	323, 1778
AsuII	TTCGAA	1 cut(s)	1862
AvaI	CYCGRG	1 cut(s)	2052
AvaII	GGWCC	2 cut(s)	292, 2297
AvrII	CCTAGG	2 cut(s)	254, 1235
BaeGI	GKGCMC	2 cut(s)	664, 2500
BaeI	ACNNNNGTAYC	6 cut(s)	984, 1017, 1896, 1929, 2185, 2218
BalI	TGGCCA	2 cut(s)	548, 1215
BanI	GGYRCC	2 cut(s)	1463, 1691
BanII	GRGCYC	1 cut(s)	180
BarI	GAAGNNNNNNTAC	2 cut(s)	1702, 1734
BauI	CACGAG	1 cut(s)	1776
BbrPI	CACGTG	1 cut(s)	1448
BbsI	GAAGAC	5 cut(s)	81, 582, 723, 1124, 1746
Bbv12I	GWGCWC	2 cut(s)	664, 2500
BbvI	GCAGC	6 cut(s)	774, 796, 925, 1059, 1159, 1850
BccI	CCATC	9 cut(s)	458, 491, 1427, 1431, 1519, 2023, 2258, 2354, 2396
BceAI	ACGGC	3 cut(s)	81, 97, 652
BciT130I	CCWGG	2 cut(s)	533, 872
BciVI	GTATCC	1 cut(s)	1372
BclI	TGATCA	1 cut(s)	2470
BcnI	CCSGG	1 cut(s)	371
BcoDI	GTCTC	3 cut(s)	588, 1707, 2086
BcuI	ACTAGT	2 cut(s)	26, 551
BfaI	CTAG	9 cut(s)	27, 173, 255, 375, 413, 552, 1236, 1640, 2180
BfmI	CTRYAG	3 cut(s)	708, 911, 1908
BfoI	RGCGCY	1 cut(s)	1695
BfuI	GTATCC	1 cut(s)	1372
BglII	AGATCT	4 cut(s)	1417, 1605, 1813, 2182
BisI	GCNGC	9 cut(s)	121, 443, 785, 788, 914, 1048, 1173, 1730, 1839
BlnI	CCTAGG	2 cut(s)	254, 1235
BlpI	GCTNAGC	2 cut(s)	179, 1503
BlsI	GCNGC	9 cut(s)	122, 444, 786, 789, 915, 1049, 1174, 1731, 1840
Bme1390I	CCNGG	3 cut(s)	371, 533, 872
Bme18I	GGWCC	2 cut(s)	292, 2297
BmeRI	GACNNNNNGTC	1 cut(s)	518
BmeT110I	CYCGRG	1 cut(s)	2052
BmgT120I	GGNCC	5 cut(s)	108, 292, 472, 2297, 2363
BmiI	GGNNCC	6 cut(s)	10, 38, 1465, 1693, 2100, 2298
BmrFI	CCNGG	3 cut(s)	371, 533, 872
BmrI	ACTGGG	2 cut(s)	607, 1666
BmsI	GCATC	8 cut(s)	761, 799, 1206, 1811, 1818, 1908, 1938, 1951
BmuI	ACTGGG	2 cut(s)	607, 1666
BpiI	GAAGAC	5 cut(s)	81, 582, 723, 1124, 1746
BpmI	CTGGAG	1 cut(s)	2189
Bpu1102I	GCTNAGC	2 cut(s)	179, 1503
Bpu14I	TTCGAA	1 cut(s)	1862
BpuEI	CTTGAG	1 cut(s)	2422
BpuMI	CCSGG	1 cut(s)	371
BsaAI	YACGTR	2 cut(s)	1448, 1550
BsaBI	GATNNNNATC	3 cut(s)	143, 1245, 1770
BsaHI	GRCGYC	2 cut(s)	839, 1692
BsaI	GGTCTC	1 cut(s)	1707
BsaJI	CCNNGG	5 cut(s)	254, 383, 1235, 1325, 1488
BsaWI	WCCGGW	2 cut(s)	11, 2196
BsaXI	ACNNNNNCTCC	2 cut(s)	314, 344
Bsc4I	CCNNNNNNNGG	9 cut(s)	1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
Bse1I	ACTGG	8 cut(s)	602, 1015, 1216, 1415, 1672, 1706, 2100, 2515
Bse3DI	GCAATG	2 cut(s)	646, 1606
Bse8I	GATNNNNATC	3 cut(s)	143, 1245, 1770
BseBI	CCWGG	2 cut(s)	533, 872
BseDI	CCNNGG	5 cut(s)	254, 383, 1235, 1325, 1488
BseGI	GGATG	8 cut(s)	1246, 1348, 1442, 1804, 1833, 2313, 2365, 2519
BseJI	GATNNNNATC	3 cut(s)	143, 1245, 1770
BseLI	CCNNNNNNNGG	9 cut(s)	1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
BseMI	GCAATG	2 cut(s)	646, 1606
BseMII	CTCAG	6 cut(s)	193, 260, 314, 1489, 1517, 2081
BseNI	ACTGG	8 cut(s)	602, 1015, 1216, 1415, 1672, 1706, 2100, 2515
BseRI	GAGGAG	3 cut(s)	372, 704, 1889
BseSI	GKGCMC	2 cut(s)	664, 2500
BseXI	GCAGC	6 cut(s)	774, 796, 925, 1059, 1159, 1850
BseYI	CCCAGC	1 cut(s)	2447
Bsh1236I	CGCG	2 cut(s)	1729, 2502
BshFI	GGCC	8 cut(s)	109, 191, 349, 473, 548, 1215, 1282, 2365
BshNI	GGYRCC	2 cut(s)	1463, 1691
BsiHKAI	GWGCWC	2 cut(s)	664, 2500
BsiHKCI	CYCGRG	1 cut(s)	2052
BsiSI	CCGG	3 cut(s)	12, 370, 2197
BslFI	GGGAC	2 cut(s)	1469, 2049
BslI	CCNNNNNNNGG	9 cut(s)	1236, 1237, 1517, 1624, 1701, 1795, 2108, 2134, 2135
BsmAI	GTCTC	3 cut(s)	588, 1707, 2086
BsmFI	GGGAC	2 cut(s)	1469, 2049
BsmI	GAATGC	1 cut(s)	1852
BsnI	GGCC	8 cut(s)	109, 191, 349, 473, 548, 1215, 1282, 2365
Bso31I	GGTCTC	1 cut(s)	1707
BsoBI	CYCGRG	1 cut(s)	2052
Bsp119I	TTCGAA	1 cut(s)	1862
Bsp1286I	GDGCHC	3 cut(s)	180, 664, 2500
Bsp1407I	TGTACA	1 cut(s)	616
Bsp1720I	GCTNAGC	2 cut(s)	179, 1503
BspACI	CCGC	7 cut(s)	94, 118, 121, 442, 986, 1729, 1742
BspANI	GGCC	8 cut(s)	109, 191, 349, 473, 548, 1215, 1282, 2365
BspCNI	CTCAG	6 cut(s)	192, 259, 313, 1488, 1516, 2082
BspFNI	CGCG	2 cut(s)	1729, 2502
BspHI	TCATGA	1 cut(s)	493
BspLI	GGNNCC	6 cut(s)	10, 38, 1465, 1693, 2100, 2298
BspMAI	CTGCAG	1 cut(s)	915
BspPI	GGATC	1 cut(s)	1139
BspT104I	TTCGAA	1 cut(s)	1862
BspT107I	GGYRCC	2 cut(s)	1463, 1691
BspTNI	GGTCTC	1 cut(s)	1707
BsrDI	GCAATG	2 cut(s)	646, 1606
BsrGI	TGTACA	1 cut(s)	616
BsrI	ACTGG	8 cut(s)	602, 1015, 1216, 1415, 1672, 1706, 2100, 2515
BssECI	CCNNGG	5 cut(s)	254, 383, 1235, 1325, 1488
BssNI	GRCGYC	2 cut(s)	839, 1692
BssSI	CACGAG	1 cut(s)	1776
BssT1I	CCWWGG	4 cut(s)	254, 1235, 1325, 1488
Bst2BI	CACGAG	1 cut(s)	1776
Bst2UI	CCWGG	2 cut(s)	533, 872
Bst4CI	ACNGT	6 cut(s)	51, 581, 709, 1752, 1912, 2466
Bst6I	CTCTTC	2 cut(s)	1615, 1705
BstACI	GRCGYC	2 cut(s)	839, 1692
BstAUI	TGTACA	1 cut(s)	616
BstBAI	YACGTR	2 cut(s)	1448, 1550
BstBI	TTCGAA	1 cut(s)	1862
BstC8I	GCNNGC	3 cut(s)	1284, 1850, 2500
BstDEI	CTNAG	9 cut(s)	179, 246, 300, 969, 1402, 1475, 1503, 1817, 2090
BstENI	CCTNNNNNAGG	1 cut(s)	1622
BstF5I	GGATG	8 cut(s)	1246, 1348, 1442, 1804, 1833, 2313, 2365, 2519
BstFNI	CGCG	2 cut(s)	1729, 2502
BstH2I	RGCGCY	1 cut(s)	1695
BstHHI	GCGC	2 cut(s)	504, 1694
BstMAI	GTCTC	3 cut(s)	588, 1707, 2086
BstMWI	GCNNNNNNNGC	3 cut(s)	1169, 1288, 1970
BstNI	CCWGG	2 cut(s)	533, 872
BstSCI	CCNGG	3 cut(s)	369, 531, 870
BstSFI	CTRYAG	3 cut(s)	708, 911, 1908
BstSLI	GKGCMC	2 cut(s)	664, 2500
BstUI	CGCG	2 cut(s)	1729, 2502
BstV1I	GCAGC	6 cut(s)	774, 796, 925, 1059, 1159, 1850
BstV2I	GAAGAC	5 cut(s)	81, 582, 723, 1124, 1746
BstX2I	RGATCY	4 cut(s)	1417, 1605, 1813, 2182
BstXI	CCANNNNNNTGG	1 cut(s)	2447
BstYI	RGATCY	4 cut(s)	1417, 1605, 1813, 2182
BsuI	GTATCC	1 cut(s)	1372
BsuRI	GGCC	8 cut(s)	109, 191, 349, 473, 548, 1215, 1282, 2365
BtsCI	GGATG	8 cut(s)	1246, 1348, 1442, 1804, 1833, 2313, 2365, 2519
BtsIMutI	CAGTG	4 cut(s)	586, 1408, 1661, 1748
Cac8I	GCNNGC	3 cut(s)	1284, 1850, 2500
CaiI	CAGNNNCTG	1 cut(s)	1750
CciI	TCATGA	1 cut(s)	493
CfoI	GCGC	2 cut(s)	504, 1694
Cfr13I	GGNCC	5 cut(s)	108, 292, 472, 2297, 2363
CseI	GACGC	3 cut(s)	172, 847, 2288
Csp6I	GTAC	2 cut(s)	617, 1059
CviQI	GTAC	2 cut(s)	617, 1059
DdeI	CTNAG	9 cut(s)	179, 246, 300, 969, 1402, 1475, 1503, 1817, 2090
DinI	GGCGCC	1 cut(s)	1693
DraI	TTTAAA	1 cut(s)	1569
DrdI	GACNNNNNNGTC	1 cut(s)	385
DriI	GACNNNNNGTC	1 cut(s)	518
DseDI	GACNNNNNNGTC	1 cut(s)	385
EaeI	YGGCCR	2 cut(s)	546, 1213
Eam1104I	CTCTTC	2 cut(s)	1615, 1705
Eam1105I	GACNNNNNGTC	1 cut(s)	518
EarI	CTCTTC	2 cut(s)	1615, 1705
EciI	GGCGGA	3 cut(s)	83, 107, 1001
Eco130I	CCWWGG	4 cut(s)	254, 1235, 1325, 1488
Eco24I	GRGCYC	1 cut(s)	180
Eco31I	GGTCTC	1 cut(s)	1707
Eco32I	GATATC	1 cut(s)	991
Eco47I	GGWCC	2 cut(s)	292, 2297
Eco72I	CACGTG	1 cut(s)	1448
Eco88I	CYCGRG	1 cut(s)	2052
EcoNI	CCTNNNNNAGG	1 cut(s)	1622
EcoRI	GAATTC	1 cut(s)	739
EcoRII	CCWGG	2 cut(s)	531, 870
EcoRV	GATATC	1 cut(s)	991
EcoT14I	CCWWGG	4 cut(s)	254, 1235, 1325, 1488
EcoT22I	ATGCAT	1 cut(s)	1953
EcoT38I	GRGCYC	1 cut(s)	180
EgeI	GGCGCC	1 cut(s)	1693
EheI	GGCGCC	1 cut(s)	1693
ErhI	CCWWGG	4 cut(s)	254, 1235, 1325, 1488
FalI	AAGNNNNNCTT	4 cut(s)	311, 343, 1300, 1332
FaqI	GGGAC	2 cut(s)	1469, 2049
FbaI	TGATCA	1 cut(s)	2470
FblI	GTMKAC	1 cut(s)	1312
Fnu4HI	GCNGC	9 cut(s)	121, 443, 785, 788, 914, 1048, 1173, 1730, 1839
FokI	GGATG	8 cut(s)	1253, 1355, 1449, 1811, 1840, 2320, 2372, 2506
FriOI	GRGCYC	1 cut(s)	180
Fsp4HI	GCNGC	9 cut(s)	121, 443, 785, 788, 914, 1048, 1173, 1730, 1839
FspBI	CTAG	9 cut(s)	27, 173, 255, 375, 413, 552, 1236, 1640, 2180
GlaI	GCGC	2 cut(s)	503, 1693
GluI	GCNGC	9 cut(s)	121, 443, 785, 788, 914, 1048, 1173, 1730, 1839
GsaI	CCCAGC	1 cut(s)	2451
GsuI	CTGGAG	1 cut(s)	2189
HaeII	RGCGCY	1 cut(s)	1695
HaeIII	GGCC	8 cut(s)	109, 191, 349, 473, 548, 1215, 1282, 2365
HapII	CCGG	3 cut(s)	12, 370, 2197
HgaI	GACGC	3 cut(s)	172, 847, 2288
HhaI	GCGC	2 cut(s)	504, 1694
Hin1I	GRCGYC	2 cut(s)	839, 1692
Hin6I	GCGC	2 cut(s)	502, 1692
HinP1I	GCGC	2 cut(s)	502, 1692
HincII	GTYRAC	2 cut(s)	529, 1313
HindII	GTYRAC	2 cut(s)	529, 1313
HindIII	AAGCTT	2 cut(s)	1865, 2547
HpaII	CCGG	3 cut(s)	12, 370, 2197
HphI	GGTGA	2 cut(s)	323, 1778
Hpy166II	GTNNAC	7 cut(s)	529, 662, 1313, 1553, 1584, 2211, 2498
Hpy8I	GTNNAC	7 cut(s)	529, 662, 1313, 1553, 1584, 2211, 2498
Hpy99I	CGWCG	6 cut(s)	71, 74, 83, 102, 158, 188
HpyAV	CCTTC	5 cut(s)	202, 1340, 2014, 2133, 2140
HpyCH4III	ACNGT	6 cut(s)	51, 581, 709, 1752, 1912, 2466
HpyCH4IV	ACGT	8 cut(s)	153, 341, 542, 771, 1039, 1447, 1549, 1804
HpyCH4V	TGCA	9 cut(s)	662, 752, 790, 913, 1838, 1852, 1951, 2237, 2498
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1169, 1288, 1970
HpyF3I	CTNAG	9 cut(s)	179, 246, 300, 969, 1402, 1475, 1503, 1817, 2090
HpySE526I	ACGT	8 cut(s)	153, 341, 542, 771, 1039, 1447, 1549, 1804
Hsp92I	GRCGYC	2 cut(s)	839, 1692
HspAI	GCGC	2 cut(s)	502, 1692
KasI	GGCGCC	1 cut(s)	1691
Ksp22I	TGATCA	1 cut(s)	2470
LmnI	GCTCC	6 cut(s)	36, 283, 1594, 1970, 2252, 2287
Lsp1109I	GCAGC	6 cut(s)	774, 796, 925, 1059, 1159, 1850
LweI	GCATC	8 cut(s)	761, 799, 1206, 1811, 1818, 1908, 1938, 1951
MaeI	CTAG	9 cut(s)	27, 173, 255, 375, 413, 552, 1236, 1640, 2180
MaeII	ACGT	8 cut(s)	153, 341, 542, 771, 1039, 1447, 1549, 1804
MaeIII	GTNAC	2 cut(s)	262, 584
MflI	RGATCY	4 cut(s)	1417, 1605, 1813, 2182
MhlI	GDGCHC	3 cut(s)	180, 664, 2500
MlsI	TGGCCA	2 cut(s)	548, 1215
MluI	ACGCGT	1 cut(s)	2500
MluNI	TGGCCA	2 cut(s)	548, 1215
Mly113I	GGCGCC	1 cut(s)	1692
MlyI	GAGTC	4 cut(s)	51, 373, 917, 1097
MmeI	TCCRAC	6 cut(s)	14, 87, 464, 754, 2072, 2495
Mox20I	TGGCCA	2 cut(s)	548, 1215
Mph1103I	ATGCAT	1 cut(s)	1953
MroXI	GAANNNNTTC	3 cut(s)	1350, 1557, 2004
MscI	TGGCCA	2 cut(s)	548, 1215
MseI	TTAA	8 cut(s)	404, 834, 903, 1125, 1568, 1914, 2012, 2534
MslI	CAYNNNNRTG	2 cut(s)	1791, 2255
Msp20I	TGGCCA	2 cut(s)	548, 1215
MspA1I	CMGCKG	3 cut(s)	787, 1301, 2153
MspI	CCGG	3 cut(s)	12, 370, 2197
MspR9I	CCNGG	3 cut(s)	371, 533, 872
Mva1269I	GAATGC	1 cut(s)	1852
MvaI	CCWGG	2 cut(s)	533, 872
MvnI	CGCG	2 cut(s)	1729, 2502
MwoI	GCNNNNNNNGC	3 cut(s)	1169, 1288, 1970
NarI	GGCGCC	1 cut(s)	1692
NciI	CCSGG	1 cut(s)	371
NlaIV	GGNNCC	6 cut(s)	10, 38, 1465, 1693, 2100, 2298
NmeAIII	GCCGAG	2 cut(s)	65, 1312
NmuCI	GTSAC	1 cut(s)	262
NsiI	ATGCAT	1 cut(s)	1953
NspV	TTCGAA	1 cut(s)	1862
OliI	CACNNNNGTG	1 cut(s)	1791
PaeR7I	CTCGAG	1 cut(s)	2052
PagI	TCATGA	1 cut(s)	493
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1852
PdmI	GAANNNNTTC	3 cut(s)	1350, 1557, 2004
PflFI	GACNNNGTC	1 cut(s)	1750
PflMI	CCANNNNNTGG	1 cut(s)	1517
PfoI	TCCNGGA	1 cut(s)	870
PkrI	GCNGC	9 cut(s)	122, 444, 786, 789, 915, 1049, 1174, 1731, 1840
PleI	GAGTC	4 cut(s)	50, 373, 917, 1097
PluTI	GGCGCC	1 cut(s)	1695
PmaCI	CACGTG	1 cut(s)	1448
PmlI	CACGTG	1 cut(s)	1448
PpsI	GAGTC	4 cut(s)	50, 373, 917, 1097
Ppu21I	YACGTR	2 cut(s)	1448, 1550
PshBI	ATTAAT	1 cut(s)	404
Psp6I	CCWGG	2 cut(s)	531, 870
PspCI	CACGTG	1 cut(s)	1448
PspFI	CCCAGC	1 cut(s)	2447
PspGI	CCWGG	2 cut(s)	531, 870
PspN4I	GGNNCC	6 cut(s)	10, 38, 1465, 1693, 2100, 2298
PspPI	GGNCC	5 cut(s)	108, 292, 472, 2297, 2363
PsrI	GAACNNNNNNTAC	2 cut(s)	2303, 2335
PstI	CTGCAG	1 cut(s)	915
PstNI	CAGNNNCTG	1 cut(s)	1750
PsuI	RGATCY	4 cut(s)	1417, 1605, 1813, 2182
PsyI	GACNNNGTC	1 cut(s)	1750
PvuII	CAGCTG	3 cut(s)	787, 1301, 2153
RsaI	GTAC	2 cut(s)	618, 1060
RsaNI	GTAC	2 cut(s)	617, 1059
RseI	CAYNNNNRTG	2 cut(s)	1791, 2255
SalI	GTCGAC	1 cut(s)	1311
SaqAI	TTAA	8 cut(s)	404, 834, 903, 1125, 1568, 1914, 2012, 2534
SatI	GCNGC	9 cut(s)	121, 443, 785, 788, 914, 1048, 1173, 1730, 1839
Sau96I	GGNCC	5 cut(s)	108, 292, 472, 2297, 2363
SchI	GAGTC	4 cut(s)	51, 373, 917, 1097
ScrFI	CCNGG	3 cut(s)	371, 533, 872
SduI	GDGCHC	3 cut(s)	180, 664, 2500
SfaNI	GCATC	8 cut(s)	761, 799, 1206, 1811, 1818, 1908, 1938, 1951
SfcI	CTRYAG	3 cut(s)	708, 911, 1908
SfoI	GGCGCC	1 cut(s)	1693
Sfr274I	CTCGAG	1 cut(s)	2052
SfuI	TTCGAA	1 cut(s)	1862
SinI	GGWCC	2 cut(s)	292, 2297
SlaI	CTCGAG	1 cut(s)	2052
SmiMI	CAYNNNNRTG	2 cut(s)	1791, 2255
SmlI	CTYRAG	2 cut(s)	2052, 2401
SmoI	CTYRAG	2 cut(s)	2052, 2401
SpeI	ACTAGT	2 cut(s)	26, 551
SsiI	CCGC	7 cut(s)	94, 118, 121, 442, 986, 1729, 1742
SspDI	GGCGCC	1 cut(s)	1691
SspI	AATATT	1 cut(s)	1429
SspMI	CTAG	9 cut(s)	27, 173, 255, 375, 413, 552, 1236, 1640, 2180
StyD4I	CCNGG	3 cut(s)	369, 531, 870
StyI	CCWWGG	4 cut(s)	254, 1235, 1325, 1488
TaaI	ACNGT	6 cut(s)	51, 581, 709, 1752, 1912, 2466
TaiI	ACGT	8 cut(s)	156, 344, 545, 774, 1042, 1450, 1552, 1807
TaqII	GACCGA	1 cut(s)	375
TatI	WGTACW	1 cut(s)	616
TauI	GCSGC	3 cut(s)	123, 445, 1732
Tru1I	TTAA	8 cut(s)	404, 834, 903, 1125, 1568, 1914, 2012, 2534
Tru9I	TTAA	8 cut(s)	404, 834, 903, 1125, 1568, 1914, 2012, 2534
TscAI	CASTG	4 cut(s)	586, 1415, 1661, 1755
TseFI	GTSAC	1 cut(s)	262
TseI	GCWGC	6 cut(s)	784, 787, 913, 1047, 1172, 1838
Tsp45I	GTSAC	1 cut(s)	262
TspRI	CASTG	4 cut(s)	586, 1415, 1661, 1755
Tth111I	GACNNNGTC	1 cut(s)	1750
Van91I	CCANNNNNTGG	1 cut(s)	1517
VneI	GTGCAC	2 cut(s)	660, 2496
VpaK11BI	GGWCC	2 cut(s)	292, 2297
VspI	ATTAAT	1 cut(s)	404
XagI	CCTNNNNNAGG	1 cut(s)	1622
XapI	RAATTY	7 cut(s)	739, 899, 1097, 1455, 1493, 1886, 1935
XcmI	CCANNNNNNNNNTGG	4 cut(s)	461, 878, 2092, 2157
XhoI	CTCGAG	1 cut(s)	2052
XmaJI	CCTAGG	2 cut(s)	254, 1235
XmiI	GTMKAC	1 cut(s)	1312
XmnI	GAANNNNTTC	3 cut(s)	1350, 1557, 2004
XspI	CTAG	9 cut(s)	27, 173, 255, 375, 413, 552, 1236, 1640, 2180
Zsp2I	ATGCAT	1 cut(s)	1953

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.