MD16G1126300.v1.1

Somatic embryogenesis receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015602
Physical Location & Seq
Reverse (-)
9193035 .. 9195896
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1126300.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGCGATCTGGGTTTTGCTGCTTCATCTTGTTGCTGCTGCCCTTGTTCTACTGTCACCCGCAACTGCAAACCTAGAAGGAGATGCTTTGTATGCTTTGAGACAAGCTGTGAAGGATCCGAACAGTGTTCTGCAGAGCTGGGATCCGACCTTGGTTGATCCCTGCACTTGGTTTCATGTCACCTGCGACGGCGACAATCGGGTTACTCGACTAGACCTTGGAAATGCAAACCTGTCGGGCAATCTTGTACCCGAGTTGGGGAGGCTCGAGCGCCTTCAGTACCTGGAATTGTACATGAACAACTTGATGGGATCTATACCAGAACAACTTGGAGGATTGAAGAGCCTTGTGAGCTTGGATCTCTACCACAATAACTTCTCTGGGTCCATCCCACCCTCCCTATCCAACCTCTCCAATCTTAAATTCTTGAGGCTGAATGATAACAGATTGACTGGAAGCATACCTAGAGAGCTTACCAAACTTCAAAGCCTCAAGATACTTGATGTATCCAACAACGATTTGTGTGGTACTTTCCCAACCACAGGCTCCTTCTCCAATTTCTCAGAAGAAAGTTTCAAGAATAACCCAAGATTGGAAGGGCCAGAACTGATGGGATTTGTGAGATATGATAGTACCAGAGGAAGCTGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8

Protein Analysis

218

Amino Acids

23.97

Weight (kDa)

5.37

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 3.6e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 71 - 169 6.3e-12 Leucine-rich repeat region
LRR_4 PF12799 91 - 132 2.5e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 139 - 174 2e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015602)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23093
malus_domestica MD13G1125500.v1.1 MD16G1126300.v1.1
prunus_persica Prupe.1G219900_v2.0.a1
pyrus_communis pycom13g10950 pycom16g10740
rosa_chinensis RchiOBHm_Chr4g0429391
rosa_laevigata RLG00000007074
rosa_multiflora Rmu_sc0003708.1_g000021
rosa_roxburghii Rroxscaffold_5G00371030
rosa_rugosa Rorug04G0235700
rosa_samantha Rh4AG291400 Rh4BG297100 Rh4CG312700 Rh4DG294200
rosa_wichuraiana Rw4G025230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 191
Acc36I ACCTGC 1 cut(s) 191
AciI CCGC 1 cut(s) 60
AclWI GGATC 7 cut(s) 110, 123, 137, 150, 152, 319, 366
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 5 cut(s) 249, 281, 293, 529, 634
AfiI CCNNNNNNNGG 5 cut(s) 168, 257, 258, 542, 592
AgsI TTSAA 3 cut(s) 340, 485, 577
AjnI CCWGG 1 cut(s) 282
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 5 cut(s) 107, 138, 354, 472, 645
AluI AGCT 5 cut(s) 107, 138, 354, 472, 645
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 7 cut(s) 110, 123, 137, 150, 152, 319, 366
AlwNI CAGNNNCTG 1 cut(s) 283
Ama87I CYCGRG 2 cut(s) 251, 266
AoxI GGCC 1 cut(s) 599
ApeKI GCWGC 4 cut(s) 19, 35, 38, 645
ApoI RAATTY 1 cut(s) 422
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 2 cut(s) 384, 599
AsuHPI GGTGA 2 cut(s) 48, 172
AvaI CYCGRG 2 cut(s) 251, 266
AvaII GGWCC 1 cut(s) 384
BamHI GGATCC 2 cut(s) 115, 142
BbvI GCAGC 4 cut(s) 6, 22, 25, 632
BccI CCATC 3 cut(s) 301, 395, 604
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 1 cut(s) 284
BciVI GTATCC 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 3 cut(s) 74, 212, 465
BfmI CTRYAG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 191
BfuI GTATCC 1 cut(s) 517
BisI GCNGC 4 cut(s) 20, 36, 39, 646
BlsI GCNGC 4 cut(s) 21, 37, 40, 647
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 384
BmeT110I CYCGRG 2 cut(s) 251, 266
BmgT120I GGNCC 2 cut(s) 384, 599
BmiI GGNNCC 4 cut(s) 117, 144, 385, 547
BmrFI CCNGG 1 cut(s) 284
BmsI GCATC 1 cut(s) 73
BpuEI CTTGAG 2 cut(s) 448, 476
BsaJI CCNNGG 2 cut(s) 150, 217
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 257, 258, 542, 592
Bse1I ACTGG 1 cut(s) 457
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 2 cut(s) 150, 217
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 5 cut(s) 168, 257, 258, 542, 592
BseMII CTCAG 1 cut(s) 576
BseNI ACTGG 1 cut(s) 457
BseXI GCAGC 4 cut(s) 6, 22, 25, 632
BseYI CCCAGC 1 cut(s) 138
BsgI GTGCAG 1 cut(s) 148
BshFI GGCC 1 cut(s) 601
BsiHKCI CYCGRG 2 cut(s) 251, 266
BslI CCNNNNNNNGG 5 cut(s) 168, 257, 258, 542, 592
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 1 cut(s) 601
BsoBI CYCGRG 2 cut(s) 251, 266
Bsp1407I TGTACA 1 cut(s) 291
Bsp143I GATC 6 cut(s) 6, 115, 142, 157, 311, 358
BspACI CCGC 1 cut(s) 60
BspANI GGCC 1 cut(s) 601
BspCNI CTCAG 1 cut(s) 575
BspLI GGNNCC 4 cut(s) 117, 144, 385, 547
BspMAI CTGCAG 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 7 cut(s) 110, 123, 137, 150, 152, 319, 366
BspQI GCTCTTC 1 cut(s) 335
BsrGI TGTACA 1 cut(s) 291
BsrI ACTGG 1 cut(s) 457
BssECI CCNNGG 2 cut(s) 150, 217
BssMI GATC 6 cut(s) 6, 115, 142, 157, 311, 358
BssT1I CCWWGG 2 cut(s) 150, 217
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 2 cut(s) 54, 125
Bst6I CTCTTC 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 562
BstF5I GGATG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 6 cut(s) 9, 118, 145, 160, 314, 361
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 6 cut(s) 6, 115, 142, 157, 311, 358
BstMWI GCNNNNNNNGC 2 cut(s) 92, 351
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 131
BstV1I GCAGC 4 cut(s) 6, 22, 25, 632
BstX2I RGATCY 4 cut(s) 115, 142, 311, 358
BstYI RGATCY 4 cut(s) 115, 142, 311, 358
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 1 cut(s) 601
BtsCI GGATG 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 130
BveI ACCTGC 1 cut(s) 191
CaiI CAGNNNCTG 1 cut(s) 283
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 384, 599
Csp6I GTAC 5 cut(s) 248, 280, 292, 528, 633
CviAII CATG 2 cut(s) 176, 295
CviQI GTAC 5 cut(s) 248, 280, 292, 528, 633
DdeI CTNAG 1 cut(s) 562
DpnI GATC 6 cut(s) 8, 117, 144, 159, 313, 360
DpnII GATC 6 cut(s) 6, 115, 142, 157, 311, 358
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 150, 217
Eco47I GGWCC 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 260
Eco88I CYCGRG 2 cut(s) 251, 266
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 2 cut(s) 150, 217
ErhI CCWWGG 2 cut(s) 150, 217
FaeI CATG 2 cut(s) 179, 298
FaiI YATR 6 cut(s) 93, 177, 296, 317, 461, 627
FatI CATG 2 cut(s) 175, 294
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 4 cut(s) 20, 36, 39, 646
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 4 cut(s) 20, 36, 39, 646
FspBI CTAG 3 cut(s) 74, 212, 465
GlaI GCGC 1 cut(s) 272
GluI GCNGC 4 cut(s) 20, 36, 39, 646
GsaI CCCAGC 1 cut(s) 142
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 1 cut(s) 601
HhaI GCGC 1 cut(s) 273
Hin1II CATG 2 cut(s) 179, 298
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HphI GGTGA 2 cut(s) 48, 172
Hpy188I TCNGA 3 cut(s) 120, 147, 565
Hpy188III TCNNGA 3 cut(s) 427, 493, 577
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 5 cut(s) 71, 106, 284, 559, 590
HpyCH4III ACNGT 2 cut(s) 54, 125
HpyCH4V TGCA 5 cut(s) 68, 133, 165, 227, 648
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 351
HpyF3I CTNAG 1 cut(s) 562
Hsp92II CATG 2 cut(s) 179, 298
HspAI GCGC 1 cut(s) 271
Kzo9I GATC 6 cut(s) 6, 115, 142, 157, 311, 358
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 1 cut(s) 551
Lsp1109I GCAGC 4 cut(s) 6, 22, 25, 632
LweI GCATC 1 cut(s) 73
MaeI CTAG 3 cut(s) 74, 212, 465
MaeIII GTNAC 3 cut(s) 54, 178, 202
MalI GATC 6 cut(s) 8, 117, 144, 159, 313, 360
MboI GATC 6 cut(s) 6, 115, 142, 157, 311, 358
MboII GAAGA 2 cut(s) 352, 578
MflI RGATCY 4 cut(s) 115, 142, 311, 358
MluCI AATT 3 cut(s) 287, 422, 556
MmeI TCCRAC 3 cut(s) 170, 429, 534
MnlI CCTC 7 cut(s) 255, 326, 406, 419, 423, 500, 632
MseI TTAA 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 284
MvaI CCWGG 1 cut(s) 284
MwoI GCNNNNNNNGC 2 cut(s) 92, 351
NdeII GATC 6 cut(s) 6, 115, 142, 157, 311, 358
NlaIII CATG 2 cut(s) 179, 298
NlaIV GGNNCC 4 cut(s) 117, 144, 385, 547
NmuCI GTSAC 2 cut(s) 54, 178
PaeR7I CTCGAG 1 cut(s) 266
PaqCI CACCTGC 1 cut(s) 191
PciSI GCTCTTC 1 cut(s) 335
PcsI WCGNNNNNNNCGW 1 cut(s) 205
PkrI GCNGC 4 cut(s) 21, 37, 40, 647
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 4 cut(s) 117, 144, 385, 547
PspPI GGNCC 2 cut(s) 384, 599
PspXI VCTCGAGB 1 cut(s) 266
PstI CTGCAG 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 283
PsuI RGATCY 4 cut(s) 115, 142, 311, 358
RsaI GTAC 5 cut(s) 249, 281, 293, 529, 634
RsaNI GTAC 5 cut(s) 248, 280, 292, 528, 633
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 4 cut(s) 20, 36, 39, 646
Sau3AI GATC 6 cut(s) 6, 115, 142, 157, 311, 358
Sau96I GGNCC 2 cut(s) 384, 599
ScrFI CCNGG 1 cut(s) 284
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 131
Sfr274I CTCGAG 1 cut(s) 266
SinI GGWCC 1 cut(s) 384
SlaI CTCGAG 1 cut(s) 266
SmlI CTYRAG 3 cut(s) 266, 427, 491
SmoI CTYRAG 3 cut(s) 266, 427, 491
Sse9I AATT 3 cut(s) 287, 422, 556
SsiI CCGC 1 cut(s) 60
SspMI CTAG 3 cut(s) 74, 212, 465
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 2 cut(s) 150, 217
TaaI ACNGT 2 cut(s) 54, 125
TaqI TCGA 2 cut(s) 208, 267
TasI AATT 3 cut(s) 287, 422, 556
TatI WGTACW 1 cut(s) 291
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 2 cut(s) 54, 178
TseI GCWGC 4 cut(s) 19, 35, 38, 645
Tsp45I GTSAC 2 cut(s) 54, 178
TspDTI ATGAA 3 cut(s) 14, 164, 311
TspRI CASTG 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 384
XapI RAATTY 1 cut(s) 422
XhoI CTCGAG 1 cut(s) 266
XspI CTAG 3 cut(s) 74, 212, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.