MD16G1153600.v1.1

Huntingtin-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015094
Physical Location & Seq
Forward (+)
12182387 .. 12183757
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1153600.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGAGGGAGGTGGAGAAGAAGGAGTCGAGATGGTGGTGGACTCCAAGGACTTGCAGCAGCAGAGCAAAGCCTTCGATAAGCTCACCGACCGCGTGGAGGATCGACAGCTTGATTCCACCCGCGTCCAGGAGGCCATGGCTTCCATCGCGGCCTCCTCCGAAGCCGACTGGAATGCTATGCGATTGAGGTCGAAGGAATTGGCTGCAGTGAAGATCAATGCCGGTGACGTTGACATAATTGCAAATGAGCTAGAGCTGGACAAGAAGGTTGCCGAGAGGACCTTGCGGGAGCACAAAGGCGATGCCGTCGCTGCCATTCGACACTTGCTTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.29

Weight (kDa)

4.95

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_HYPK PF19026 70 - 110 1.3e-17 HYPK UBA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015094)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06610
fragaria_vesca FvH4_4g19820
malus_domestica MD13G1153200.v1.1 MD16G1153600.v1.1
prunus_persica Prupe.1G116800_v2.0.a1
pyrus_communis pycom16g13040
rosa_chinensis RchiOBHm_Chr4g0425361
rosa_laevigata RLG00000007373
rosa_multiflora Rmu_sc0001900.1_g000049
rosa_roxburghii Rroxscaffold_5G00367350
rosa_rugosa Rorug04G0202500
rosa_samantha Rh4AG259700 Rh4BG265600 Rh4CG276900 Rh4DG261600
rosa_wichuraiana Rw4G022480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 93, 123, 149
AciI CCGC 4 cut(s) 91, 121, 149, 286
AclWI GGATC 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 97, 127
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 4 cut(s) 82, 109, 250, 256
AluI AGCT 4 cut(s) 82, 109, 250, 256
Alw21I GWGCWC 1 cut(s) 294
AlwI GGATC 1 cut(s) 108
AoxI GGCC 2 cut(s) 132, 150
ApeKI GCWGC 4 cut(s) 55, 58, 203, 311
ArsI GACNNNNNNTTYG 1 cut(s) 312
AspS9I GGNCC 1 cut(s) 279
AsuHPI GGTGA 2 cut(s) 76, 236
AvaII GGWCC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 4 cut(s) 67, 70, 190, 298
BccI CCATC 2 cut(s) 25, 152
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 4 cut(s) 55, 89, 155, 189
BciT130I CCWGG 1 cut(s) 128
BfaI CTAG 2 cut(s) 251, 334
BfmI CTRYAG 1 cut(s) 204
BisI GCNGC 5 cut(s) 56, 59, 150, 204, 312
BlsI GCNGC 5 cut(s) 57, 60, 151, 205, 313
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 45, 135
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 127
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 2 cut(s) 45, 135
BseLI CCNNNNNNNGG 2 cut(s) 97, 127
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 4 cut(s) 67, 70, 190, 298
Bsh1236I CGCG 3 cut(s) 93, 123, 149
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 2 cut(s) 134, 152
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 97, 127
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 2 cut(s) 134, 152
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 2 cut(s) 100, 213
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 4 cut(s) 91, 121, 149, 286
BspANI GGCC 2 cut(s) 134, 152
BspFNI CGCG 3 cut(s) 93, 123, 149
BspMAI CTGCAG 1 cut(s) 208
BspPI GGATC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 45, 135
BssMI GATC 2 cut(s) 100, 213
BssT1I CCWWGG 2 cut(s) 45, 135
Bst2UI CCWGG 1 cut(s) 128
BstDSI CCRYGG 1 cut(s) 135
BstFNI CGCG 3 cut(s) 93, 123, 149
BstKTI GATC 2 cut(s) 103, 216
BstMBI GATC 2 cut(s) 100, 213
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 2 cut(s) 146, 311
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 204
BstUI CGCG 3 cut(s) 93, 123, 149
BstV1I GCAGC 4 cut(s) 67, 70, 190, 298
BsuRI GGCC 2 cut(s) 134, 152
BtgI CCRYGG 1 cut(s) 135
BtgZI GCGATG 2 cut(s) 130, 315
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 213
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 279
CseI GACGC 1 cut(s) 112
CviAII CATG 1 cut(s) 136
DpnI GATC 2 cut(s) 102, 215
DpnII GATC 2 cut(s) 100, 213
Eco130I CCWWGG 2 cut(s) 45, 135
Eco47I GGWCC 1 cut(s) 279
EcoO109I RGGNCCY 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 126
EcoT14I CCWWGG 2 cut(s) 45, 135
ErhI CCWWGG 2 cut(s) 45, 135
FaeI CATG 1 cut(s) 139
FaiI YATR 3 cut(s) 137, 179, 236
FatI CATG 1 cut(s) 135
FauI CCCGC 2 cut(s) 128, 279
Fnu4HI GCNGC 5 cut(s) 56, 59, 150, 204, 312
Fsp4HI GCNGC 5 cut(s) 56, 59, 150, 204, 312
FspBI CTAG 2 cut(s) 251, 334
GluI GCNGC 5 cut(s) 56, 59, 150, 204, 312
HaeIII GGCC 2 cut(s) 134, 152
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 139
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 3 cut(s) 24, 41, 113
HpaII CCGG 1 cut(s) 222
HphI GGTGA 2 cut(s) 76, 236
Hpy166II GTNNAC 2 cut(s) 40, 232
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 1 cut(s) 28
Hpy8I GTNNAC 2 cut(s) 40, 232
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 14, 82, 187, 259
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 3 cut(s) 55, 206, 242
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 311
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 1 cut(s) 139
Kzo9I GATC 2 cut(s) 100, 213
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 5 cut(s) 113, 140, 154, 235, 242
Lsp1109I GCAGC 4 cut(s) 67, 70, 190, 298
LweI GCATC 1 cut(s) 292
MaeI CTAG 2 cut(s) 251, 334
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 102, 215
MboI GATC 2 cut(s) 100, 213
MboII GAAGA 2 cut(s) 29, 223
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 2 cut(s) 197, 237
MlyI GAGTC 2 cut(s) 33, 35
MnlI CCTC 6 cut(s) 91, 124, 163, 166, 180, 270
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 128
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 3 cut(s) 93, 123, 149
MwoI GCNNNNNNNGC 2 cut(s) 146, 311
NcoI CCATGG 1 cut(s) 135
NdeII GATC 2 cut(s) 100, 213
NlaIII CATG 1 cut(s) 139
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 224
PctI GAATGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 113
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 5 cut(s) 57, 60, 151, 205, 313
PleI GAGTC 2 cut(s) 32, 35
PpsI GAGTC 2 cut(s) 32, 35
PpuMI RGGWCCY 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspPI GGNCC 1 cut(s) 279
PspPPI RGGWCCY 1 cut(s) 279
PstI CTGCAG 1 cut(s) 208
SatI GCNGC 5 cut(s) 56, 59, 150, 204, 312
Sau3AI GATC 2 cut(s) 100, 213
Sau96I GGNCC 1 cut(s) 279
SchI GAGTC 2 cut(s) 33, 35
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 294
SetI ASST 9 cut(s) 13, 84, 111, 191, 231, 252, 258, 270, 284
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 279
Sse9I AATT 2 cut(s) 197, 237
SsiI CCGC 4 cut(s) 91, 121, 149, 286
SspMI CTAG 2 cut(s) 251, 334
StyD4I CCNGG 1 cut(s) 126
StyI CCWWGG 2 cut(s) 45, 135
TaiI ACGT 1 cut(s) 231
TaqI TCGA 5 cut(s) 27, 75, 103, 191, 319
TasI AATT 2 cut(s) 197, 237
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 113
TscAI CASTG 1 cut(s) 213
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 4 cut(s) 55, 58, 203, 311
Tsp45I GTSAC 1 cut(s) 224
TspRI CASTG 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 279
XspI CTAG 2 cut(s) 251, 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.