MD16G1239900.v1.1
HSP70 Family

Hsp70 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0021166
Physical Location & Seq
Reverse (-)
25490242 .. 25491033
792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1239900.v1.1.491

Sequence Viewer

Length: 792 bp
ATGAACCCTATTAACACCGTCTTCGATGCTAAGCGGTTGATTGGTCGTAGAGTTACCGATGCCTCTGTCCAGAGCGACATGAAGTTGTGGCCATTCAAGGTTACCTCCGGGCCCGGTGACAAGCGCATGATTGGAGTCTTTTACAAGGAAGAAGATAAGCTCTTCGCCGCTGAGGAAATCTCCTCCATGGTCCTTATCAAGATGCATGAGATCACCGAGGCCTACCTTGGCAGCACCATCAAGAACGCTGTTGTCACAGTTCCTGCCTACTTTAACAACTCCCAGCGTCAAGCAACCAAGGATGCTGGAGTTATTGCCGAAATCAATGTCCTCCATATCATCAATGAGCCCACTGCTGCCGCCATTGCTTATGGCCTTGACAAGAAGGCCACCAACGTTGGTGAGAAGAATGTCTTGATCTTCGATCTTAGAGGTGGTACCTTAGATGTGTCTCTTCTCACCATTGAGAAGGGTATCTTTGAGGTTAAGGCCACAACCAATGACACCCAGTTGGGAGGAGAAGATTTTGATAACAGAATGGAAAACCACTTCGTTCAGGAGTTCAAGAGGAAGAACAAGAAGGATATCAGTGGCAACCCCAGAGCACTCAGGAGGTTGATGACATCCTATGAAAGGGCAAAGAGAACCCTCTCTTCCACCGCGCAGACCACCATCGAAATCGACTCCCTTTATAAGGGTATTGATTTCTACTCCACCATCACCCGTGCTAGATTCGAGGAGTTGAACGTGGATTTTTCAGGAAGTGTATGGAACCAATCGAGAAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

264

Amino Acids

29.52

Weight (kDa)

8.6

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 1 - 252 3e-96 Hsp70 protein
MreB_Mbl PF06723 79 - 188 1.5e-06 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021166)

Species Orthologous Gene IDs
malus_domestica MD16G1239900.v1.1
rosa_chinensis RchiOBHm_Chr6g0245001 RchiOBHm_Chr7g0201211
rosa_rugosa Rorug07G0062300
rosa_samantha Rh7AG189400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
Acc65I GGTACC 1 cut(s) 437
AccB1I GGYRCC 1 cut(s) 437
AccII CGCG 1 cut(s) 662
AciI CCGC 4 cut(s) 34, 168, 360, 660
AclI AACGTT 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 89
AfaI GTAC 1 cut(s) 439
AfiI CCNNNNNNNGG 2 cut(s) 633, 694
AgsI TTSAA 3 cut(s) 97, 565, 745
AluBI AGCT 1 cut(s) 160
AluI AGCT 1 cut(s) 160
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 6 cut(s) 89, 110, 219, 373, 387, 489
ApaI GGGCCC 1 cut(s) 114
ApeKI GCWGC 2 cut(s) 231, 356
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
Asp718I GGTACC 1 cut(s) 437
AspLEI GCGC 2 cut(s) 126, 664
AspS9I GGNCC 3 cut(s) 110, 111, 190
AsuC2I CCSGG 2 cut(s) 109, 114
AsuHPI GGTGA 5 cut(s) 128, 205, 413, 451, 712
AvaII GGWCC 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 91
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 2 cut(s) 114, 351
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 607
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 243, 343
BccI CCATC 3 cut(s) 245, 680, 725
BcnI CCSGG 2 cut(s) 109, 114
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 1 cut(s) 729
BisI GCNGC 4 cut(s) 168, 232, 357, 360
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 4 cut(s) 169, 233, 358, 361
Bme1390I CCNGG 2 cut(s) 109, 114
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 3 cut(s) 110, 111, 190
BmiI GGNNCC 3 cut(s) 112, 439, 773
BmrFI CCNGG 2 cut(s) 109, 114
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 4 cut(s) 16, 49, 192, 292
BmuI ACTGGG 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 13
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 171
Bpu1102I GCTNAGC 1 cut(s) 30
BpuMI CCSGG 2 cut(s) 109, 114
BsaJI CCNNGG 4 cut(s) 186, 216, 226, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 633, 694
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 363
BseDI CCNNGG 4 cut(s) 186, 216, 226, 297
BseGI GGATG 2 cut(s) 307, 623
BseLI CCNNNNNNNGG 2 cut(s) 633, 694
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 2 cut(s) 162, 622
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 3 cut(s) 172, 531, 752
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 2 cut(s) 243, 343
BseYI CCCAGC 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 662
BshFI GGCC 6 cut(s) 91, 112, 221, 375, 389, 491
BshNI GGYRCC 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 607
BsiSI CCGG 2 cut(s) 108, 114
BslI CCNNNNNNNGG 2 cut(s) 633, 694
BsmAI GTCTC 1 cut(s) 456
BsnI GGCC 6 cut(s) 91, 112, 221, 375, 389, 491
Bsp120I GGGCCC 1 cut(s) 110
Bsp1286I GDGCHC 3 cut(s) 114, 351, 607
Bsp143I GATC 3 cut(s) 210, 417, 424
Bsp1720I GCTNAGC 1 cut(s) 30
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 4 cut(s) 34, 168, 360, 660
BspANI GGCC 6 cut(s) 91, 112, 221, 375, 389, 491
BspCNI CTCAG 2 cut(s) 163, 621
BspFNI CGCG 1 cut(s) 662
BspLI GGNNCC 3 cut(s) 112, 439, 773
BspQI GCTCTTC 1 cut(s) 167
BspT107I GGYRCC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 4 cut(s) 186, 216, 226, 297
BssMI GATC 3 cut(s) 210, 417, 424
BssT1I CCWWGG 3 cut(s) 186, 226, 297
Bst4CI ACNGT 2 cut(s) 19, 259
Bst6I CTCTTC 3 cut(s) 167, 459, 658
BstDEI CTNAG 5 cut(s) 30, 171, 428, 442, 608
BstDSI CCRYGG 1 cut(s) 186
BstEII GGTNACC 1 cut(s) 100
BstENI CCTNNNNNAGG 2 cut(s) 631, 692
BstF5I GGATG 2 cut(s) 307, 623
BstFNI CGCG 1 cut(s) 662
BstHHI GCGC 2 cut(s) 126, 664
BstKTI GATC 3 cut(s) 213, 420, 427
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 3 cut(s) 210, 417, 424
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 107, 112
BstSLI GKGCMC 1 cut(s) 114
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 2 cut(s) 243, 343
BstV2I GAAGAC 1 cut(s) 13
BsuRI GGCC 6 cut(s) 91, 112, 221, 375, 389, 491
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 307, 623
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 2 cut(s) 351, 595
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 2 cut(s) 126, 664
Cfr13I GGNCC 3 cut(s) 110, 111, 190
CseI GACGC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 438
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviAII CATG 4 cut(s) 79, 127, 187, 206
CviJI RGCY 8 cut(s) 91, 112, 160, 221, 349, 375, 389, 491
CviKI_1 RGCY 8 cut(s) 91, 112, 160, 221, 349, 375, 389, 491
CviQI GTAC 1 cut(s) 438
DdeI CTNAG 5 cut(s) 30, 171, 428, 442, 608
DpnI GATC 3 cut(s) 212, 419, 426
DpnII GATC 3 cut(s) 210, 417, 424
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 3 cut(s) 167, 459, 658
EarI CTCTTC 3 cut(s) 167, 459, 658
Eco130I CCWWGG 3 cut(s) 186, 226, 297
Eco147I AGGCCT 1 cut(s) 221
Eco24I GRGCYC 2 cut(s) 114, 351
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 190
Eco91I GGTNACC 1 cut(s) 100
EcoNI CCTNNNNNAGG 2 cut(s) 631, 692
EcoO65I GGTNACC 1 cut(s) 100
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 3 cut(s) 186, 226, 297
EcoT22I ATGCAT 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 114, 351
ErhI CCWWGG 3 cut(s) 186, 226, 297
FaeI CATG 4 cut(s) 82, 130, 190, 209
FaiI YATR 9 cut(s) 80, 128, 188, 207, 336, 372, 630, 693, 769
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 461, 493
FatI CATG 4 cut(s) 78, 126, 186, 205
Fnu4HI GCNGC 4 cut(s) 168, 232, 357, 360
FokI GGATG 2 cut(s) 314, 610
FriOI GRGCYC 2 cut(s) 114, 351
Fsp4HI GCNGC 4 cut(s) 168, 232, 357, 360
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 125, 663
GluI GCNGC 4 cut(s) 168, 232, 357, 360
GsaI CCCAGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 6 cut(s) 91, 112, 221, 375, 389, 491
HapII CCGG 2 cut(s) 108, 114
HgaI GACGC 1 cut(s) 275
HhaI GCGC 2 cut(s) 126, 664
Hin1II CATG 4 cut(s) 82, 130, 190, 209
Hin6I GCGC 2 cut(s) 124, 662
HinP1I GCGC 2 cut(s) 124, 662
HinfI GANTC 3 cut(s) 135, 683, 732
HpaII CCGG 2 cut(s) 108, 114
HphI GGTGA 5 cut(s) 128, 205, 413, 451, 712
Hpy188III TCNNGA 9 cut(s) 70, 199, 241, 415, 557, 565, 610, 759, 780
HpyAV CCTTC 3 cut(s) 379, 463, 574
HpyCH4III ACNGT 2 cut(s) 19, 259
HpyCH4IV ACGT 2 cut(s) 396, 747
HpyCH4V TGCA 1 cut(s) 205
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 5 cut(s) 30, 171, 428, 442, 608
HpySE526I ACGT 2 cut(s) 396, 747
Hsp92II CATG 4 cut(s) 82, 130, 190, 209
HspAI GCGC 2 cut(s) 124, 662
KpnI GGTACC 1 cut(s) 441
Kzo9I GATC 3 cut(s) 210, 417, 424
LguI GCTCTTC 1 cut(s) 167
Lsp1109I GCAGC 2 cut(s) 243, 343
LweI GCATC 4 cut(s) 16, 49, 192, 292
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 396, 747
MaeIII GTNAC 4 cut(s) 52, 100, 116, 253
MalI GATC 3 cut(s) 212, 419, 426
MboI GATC 3 cut(s) 210, 417, 424
MhlI GDGCHC 3 cut(s) 114, 351, 607
MlsI TGGCCA 1 cut(s) 91
MluNI TGGCCA 1 cut(s) 91
MlyI GAGTC 2 cut(s) 144, 677
Mox20I TGGCCA 1 cut(s) 91
Mph1103I ATGCAT 1 cut(s) 207
MscI TGGCCA 1 cut(s) 91
MseI TTAA 3 cut(s) 12, 273, 486
Msp20I TGGCCA 1 cut(s) 91
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 108, 114
MspR9I CCNGG 2 cut(s) 109, 114
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 1 cut(s) 365
NciI CCSGG 2 cut(s) 109, 114
NcoI CCATGG 1 cut(s) 186
NdeII GATC 3 cut(s) 210, 417, 424
NlaIII CATG 4 cut(s) 82, 130, 190, 209
NlaIV GGNNCC 3 cut(s) 112, 439, 773
NmuCI GTSAC 2 cut(s) 116, 253
NsiI ATGCAT 1 cut(s) 207
PceI AGGCCT 1 cut(s) 221
PciSI GCTCTTC 1 cut(s) 167
PfeI GAWTC 1 cut(s) 732
PkrI GCNGC 4 cut(s) 169, 233, 358, 361
PleI GAGTC 2 cut(s) 143, 677
PpsI GAGTC 2 cut(s) 143, 677
PsiI TTATAA 1 cut(s) 693
Psp1406I AACGTT 1 cut(s) 396
PspEI GGTNACC 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 282
PspN4I GGNNCC 3 cut(s) 112, 439, 773
PspOMI GGGCCC 1 cut(s) 110
PspPI GGNCC 3 cut(s) 110, 111, 190
PstNI CAGNNNCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 439
RsaNI GTAC 1 cut(s) 438
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 3 cut(s) 12, 273, 486
SatI GCNGC 4 cut(s) 168, 232, 357, 360
Sau3AI GATC 3 cut(s) 210, 417, 424
Sau96I GGNCC 3 cut(s) 110, 111, 190
SchI GAGTC 2 cut(s) 144, 677
ScrFI CCNGG 2 cut(s) 109, 114
SduI GDGCHC 3 cut(s) 114, 351, 607
SfaNI GCATC 4 cut(s) 16, 49, 192, 292
SinI GGWCC 1 cut(s) 190
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 4 cut(s) 34, 168, 360, 660
SspMI CTAG 1 cut(s) 729
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 2 cut(s) 107, 112
StyI CCWWGG 3 cut(s) 186, 226, 297
TaaI ACNGT 2 cut(s) 19, 259
TaiI ACGT 2 cut(s) 399, 750
TaqI TCGA 6 cut(s) 24, 423, 675, 681, 735, 779
TauI GCSGC 2 cut(s) 170, 362
TfiI GAWTC 1 cut(s) 732
Tru1I TTAA 3 cut(s) 12, 273, 486
Tru9I TTAA 3 cut(s) 12, 273, 486
TscAI CASTG 2 cut(s) 358, 595
TseFI GTSAC 2 cut(s) 116, 253
TseI GCWGC 2 cut(s) 231, 356
Tsp45I GTSAC 2 cut(s) 116, 253
TspDTI ATGAA 3 cut(s) 17, 95, 645
TspRI CASTG 2 cut(s) 358, 595
VpaK11BI GGWCC 1 cut(s) 190
XagI CCTNNNNNAGG 2 cut(s) 631, 692
XspI CTAG 1 cut(s) 729
Zsp2I ATGCAT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.