Rh7AG189400
HSP70 Family

heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0021166
Physical Location & Seq
Reverse (-)
16931102 .. 16931598
497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG189400.1

Sequence Viewer

Length: 405 bp
ATGATTGTGATTAACTACAAGAATGAAGAGAAAAAGTTTTCTGCTGAGGAGCATACTTTAATGACTTCCAACGCCGGCCAGGCTACAAAAGATGCTGGTATAATTGCAGGTATGAATGTCATGCGCATCATAAATGAGCCTACCGCTACTGCTATTGCCTATGGTCTTGACAAAAAAGGAAGCACTGTTGGTGGAAAGAACGTTCTTATTTTTGATCTTGGTGGTGGCACTTTCGATGTTTCCCTTCTCAAAATTGAAGAGGGAATTTTCGAGGTGCAAGCTACTGCTGGAGATTCCCATCTTGGAGGTGAAGCTTTTGACAACCGAATGGTGAACCATTTTGTGCAAGAGTTTAAGAGGAAACACAAAAAAGATATAAGCAATAACCCCAAATTAAAGCACTAA

Protein Analysis

134

Amino Acids

14.65

Weight (kDa)

6.97

Isoelectric Point (pI)

23.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 27 - 131 5.1e-49 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021166)

Species Orthologous Gene IDs
malus_domestica MD16G1239900.v1.1
rosa_chinensis RchiOBHm_Chr6g0245001 RchiOBHm_Chr7g0201211
rosa_rugosa Rorug07G0062300
rosa_samantha Rh7AG189400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 125
Acc36I ACCTGC 1 cut(s) 98
AciI CCGC 1 cut(s) 144
AclI AACGTT 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 1 cut(s) 264
AgsI TTSAA 1 cut(s) 257
AjnI CCWGG 1 cut(s) 78
AloI GAACNNNNNNTCC 2 cut(s) 186, 218
AluBI AGCT 2 cut(s) 281, 314
AluI AGCT 2 cut(s) 281, 314
AoxI GGCC 1 cut(s) 76
ApoI RAATTY 1 cut(s) 264
AspLEI GCGC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 320, 343
BbvCI CCTCAGC 1 cut(s) 45
BccI CCATC 1 cut(s) 306
BciT130I CCWGG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 98
BglI GCCNNNNNGGC 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 80
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 82, 135
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 297
Bse118I RCCGGY 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 1 cut(s) 80
BseJI GATNNNNATC 1 cut(s) 297
BseMII CTCAG 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 62
BshFI GGCC 1 cut(s) 78
BsiSI CCGG 1 cut(s) 75
BsnI GGCC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 214
BspACI CCGC 1 cut(s) 144
BspANI GGCC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssMI GATC 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 2 cut(s) 21, 252
BstC8I GCNNGC 2 cut(s) 76, 279
BstDEI CTNAG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BsuRI GGCC 1 cut(s) 78
BtsIMutI CAGTG 1 cut(s) 183
BveI ACCTGC 1 cut(s) 98
Cac8I GCNNGC 2 cut(s) 76, 279
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 74
CviAII CATG 1 cut(s) 121
CviJI RGCY 5 cut(s) 78, 83, 139, 281, 314
CviKI_1 RGCY 5 cut(s) 78, 83, 139, 281, 314
DdeI CTNAG 1 cut(s) 45
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 2 cut(s) 21, 252
EarI CTCTTC 2 cut(s) 21, 252
EcoRII CCWGG 1 cut(s) 78
FaeI CATG 1 cut(s) 124
FaiI YATR 7 cut(s) 54, 101, 113, 122, 131, 162, 377
FatI CATG 1 cut(s) 120
FspAI RTGCGCAY 1 cut(s) 125
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 1 cut(s) 125
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 75
HhaI GCGC 1 cut(s) 126
Hin1II CATG 1 cut(s) 124
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 1 cut(s) 293
HpaII CCGG 1 cut(s) 75
HphI GGTGA 2 cut(s) 320, 343
Hpy166II GTNNAC 1 cut(s) 334
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 107, 277, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 124
HspAI GCGC 1 cut(s) 124
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Kzo9I GATC 1 cut(s) 214
LmnI GCTCC 1 cut(s) 49
LpnPI CCDG 6 cut(s) 65, 81, 88, 92, 93, 273
LweI GCATC 2 cut(s) 82, 135
MaeII ACGT 1 cut(s) 201
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 2 cut(s) 38, 269
MluCI AATT 4 cut(s) 102, 252, 264, 392
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 5 cut(s) 40, 253, 265, 299, 351
MroNI GCCGGC 1 cut(s) 74
MseI TTAA 4 cut(s) 12, 59, 354, 395
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 80
NaeI GCCGGC 1 cut(s) 76
NdeII GATC 1 cut(s) 214
NgoMIV GCCGGC 1 cut(s) 74
NlaIII CATG 1 cut(s) 124
NsbI TGCGCA 1 cut(s) 125
PdiI GCCGGC 1 cut(s) 76
PfeI GAWTC 1 cut(s) 293
Psp1406I AACGTT 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
SaqAI TTAA 4 cut(s) 12, 59, 354, 395
Sau3AI GATC 1 cut(s) 214
ScrFI CCNGG 1 cut(s) 80
SetI ASST 6 cut(s) 112, 204, 276, 283, 310, 316
SfaNI GCATC 2 cut(s) 82, 135
Sse9I AATT 4 cut(s) 102, 252, 264, 392
SsiI CCGC 1 cut(s) 144
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 234, 270
TasI AATT 4 cut(s) 102, 252, 264, 392
TfiI GAWTC 1 cut(s) 293
Tru1I TTAA 4 cut(s) 12, 59, 354, 395
Tru9I TTAA 4 cut(s) 12, 59, 354, 395
TscAI CASTG 1 cut(s) 190
TspDTI ATGAA 2 cut(s) 39, 128
TspRI CASTG 1 cut(s) 190
XapI RAATTY 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.