MD16G1262200.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0020170
Physical Location & Seq
Forward (+)
32649292 .. 32650427
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1262200.v1.1.491

Sequence Viewer

Length: 501 bp
ATGACCAACTTAGACAGAAAGAAGGCTATCAAGTTATCAGCAGATATAGCCATGGCTTCTACAAGAAATGGAACTACATTTTGGAGCCGCGCCGTCATAGCCAATGCTACCCACAGCAACGGCGACAATAACAGAATCTTCGTTGAGCGTACACTGGGTGGTGGTGATGGTGGTGGTGGTCGTTTGTCCGAGCACTGTGGGAGAACGAAAGCTTCGAGATCACTGTCGTCAGTGATGCACAAGAAGATCTTGAAGAAAAGCCGGAGGGTATGTAGTAGAACTGTGTTGGGGAGGAAACATAACCTAGCAAAAACCCATTCGATTGCCAAGAGATTGGTCCGGAAGAAAACACAAGTGCTCAAGAGTCTTGTTCCTGGAGGAGAATCCATGGATGAGCTCTCTTTGGTTGTGGAAACCCTAGATTATATCGCGTCTCTGCGAGCTCAGGTTGAGGTAATGCGGTGTCTTGCCACCACCGCGGAGTTATTAAATGGTACATAA

Protein Analysis

167

Amino Acids

18.09

Weight (kDa)

10.67

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBH1_N PF26576 3 - 38 4e-07 IBH1-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30410 AT4G30410
malus_domestica MD13G1260000.v1.1 MD16G1262200.v1.1
prunus_persica Prupe.1G097600_v2.0.a1
pyrus_communis pycom16g23100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 90, 431, 479
AccIII TCCGGA 1 cut(s) 339
AciI CCGC 4 cut(s) 88, 460, 477, 479
AdeI CACNNNGTG 1 cut(s) 158
AfaI GTAC 2 cut(s) 151, 496
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 373
AjuI GAANNNNNNNTTGG 2 cut(s) 64, 96
AluBI AGCT 3 cut(s) 212, 397, 443
AluI AGCT 3 cut(s) 212, 397, 443
Alw21I GWGCWC 4 cut(s) 195, 360, 399, 445
Alw26I GTCTC 1 cut(s) 438
Aor13HI TCCGGA 1 cut(s) 339
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 1 cut(s) 176
AvaII GGWCC 1 cut(s) 337
BanII GRGCYC 2 cut(s) 399, 445
Bbv12I GWGCWC 4 cut(s) 195, 360, 399, 445
BccI CCATC 1 cut(s) 161
BceAI ACGGC 2 cut(s) 77, 136
BciT130I CCWGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 2 cut(s) 305, 419
BglII AGATCT 1 cut(s) 246
BisI GCNGC 1 cut(s) 88
BlsI GCNGC 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 375
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 375
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 164
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 51, 387, 477
BsaWI WCCGGW 1 cut(s) 339
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bse1I ACTGG 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 339
BseBI CCWGG 1 cut(s) 375
BseDI CCNNGG 3 cut(s) 51, 387, 477
BseGI GGATG 1 cut(s) 397
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 393
Bsh1236I CGCG 3 cut(s) 90, 431, 479
BsiHKAI GWGCWC 4 cut(s) 195, 360, 399, 445
BsiSI CCGG 2 cut(s) 262, 340
BsmAI GTCTC 1 cut(s) 438
BsmBI CGTCTC 1 cut(s) 438
Bsp1286I GDGCHC 4 cut(s) 195, 360, 399, 445
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 2 cut(s) 218, 246
Bsp19I CCATGG 2 cut(s) 51, 387
BspACI CCGC 4 cut(s) 88, 460, 477, 479
BspCNI CTCAG 1 cut(s) 457
BspEI TCCGGA 1 cut(s) 339
BspFNI CGCG 3 cut(s) 90, 431, 479
BspLI GGNNCC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 159
BssECI CCNNGG 3 cut(s) 51, 387, 477
BssMI GATC 2 cut(s) 218, 246
BssT1I CCWWGG 2 cut(s) 51, 387
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 3 cut(s) 197, 225, 283
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 2 cut(s) 10, 444
BstDSI CCRYGG 3 cut(s) 51, 387, 477
BstF5I GGATG 1 cut(s) 397
BstFNI CGCG 3 cut(s) 90, 431, 479
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 2 cut(s) 221, 249
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 2 cut(s) 218, 246
BstMWI GCNNNNNNNGC 3 cut(s) 47, 98, 476
BstNI CCWGG 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 373
BstUI CGCG 3 cut(s) 90, 431, 479
BstX2I RGATCY 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 334
BstYI RGATCY 1 cut(s) 246
BtgI CCRYGG 3 cut(s) 51, 387, 477
BtsCI GGATG 1 cut(s) 397
BtsIMutI CAGTG 4 cut(s) 152, 193, 221, 237
Cac8I GCNNGC 1 cut(s) 441
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 337
Cfr42I CCGCGG 1 cut(s) 480
CseI GACGC 1 cut(s) 420
Csp6I GTAC 2 cut(s) 150, 495
CviAII CATG 2 cut(s) 52, 388
CviJI RGCY 9 cut(s) 26, 50, 56, 87, 101, 212, 261, 397, 443
CviKI_1 RGCY 9 cut(s) 26, 50, 56, 87, 101, 212, 261, 397, 443
CviQI GTAC 2 cut(s) 150, 495
DdeI CTNAG 2 cut(s) 10, 444
DpnI GATC 2 cut(s) 220, 248
DpnII GATC 2 cut(s) 218, 246
DraIII CACNNNGTG 1 cut(s) 158
Ecl136II GAGCTC 2 cut(s) 397, 443
Eco130I CCWWGG 2 cut(s) 51, 387
Eco24I GRGCYC 2 cut(s) 399, 445
Eco47I GGWCC 1 cut(s) 337
Eco53kI GAGCTC 2 cut(s) 397, 443
EcoICRI GAGCTC 2 cut(s) 397, 443
EcoRII CCWGG 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 51, 387
EcoT38I GRGCYC 2 cut(s) 399, 445
ErhI CCWWGG 2 cut(s) 51, 387
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 2 cut(s) 55, 391
FaiI YATR 8 cut(s) 47, 53, 98, 271, 300, 389, 426, 499
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FatI CATG 2 cut(s) 51, 387
Fnu4HI GCNGC 1 cut(s) 88
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 2 cut(s) 399, 445
Fsp4HI GCNGC 1 cut(s) 88
FspBI CTAG 2 cut(s) 305, 419
GlaI GCGC 1 cut(s) 91
GluI GCNGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 396
HapII CCGG 2 cut(s) 262, 340
HgaI GACGC 1 cut(s) 420
HhaI GCGC 1 cut(s) 92
Hin1II CATG 2 cut(s) 55, 391
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 3 cut(s) 135, 364, 383
HpaII CCGG 2 cut(s) 262, 340
HphI GGTGA 1 cut(s) 176
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 4 cut(s) 216, 250, 340, 361
Hpy8I GTNNAC 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 3 cut(s) 197, 225, 283
HpyCH4V TGCA 1 cut(s) 238
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 98, 476
HpyF3I CTNAG 2 cut(s) 10, 444
Hsp92II CATG 2 cut(s) 55, 391
HspAI GCGC 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 339
KspI CCGCGG 1 cut(s) 480
Kzo9I GATC 2 cut(s) 218, 246
LmnI GCTCC 1 cut(s) 84
LpnPI CCDG 6 cut(s) 140, 275, 353, 360, 387, 431
LweI GCATC 1 cut(s) 225
MaeI CTAG 2 cut(s) 305, 419
MalI GATC 2 cut(s) 220, 248
MboI GATC 2 cut(s) 218, 246
MboII GAAGA 4 cut(s) 130, 256, 265, 355
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 4 cut(s) 195, 360, 399, 445
MlyI GAGTC 1 cut(s) 373
MnlI CCTC 4 cut(s) 258, 285, 371, 445
MroI TCCGGA 1 cut(s) 339
MseI TTAA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 2 cut(s) 262, 340
MspR9I CCNGG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 375
MvnI CGCG 3 cut(s) 90, 431, 479
MwoI GCNNNNNNNGC 3 cut(s) 47, 98, 476
NcoI CCATGG 2 cut(s) 51, 387
NdeII GATC 2 cut(s) 218, 246
NlaIII CATG 2 cut(s) 55, 391
NlaIV GGNNCC 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PfeI GAWTC 2 cut(s) 135, 383
PfoI TCCNGGA 1 cut(s) 373
PkrI GCNGC 1 cut(s) 89
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
Psp124BI GAGCTC 2 cut(s) 399, 445
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 86
PspPI GGNCC 1 cut(s) 337
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 2 cut(s) 151, 496
RsaNI GTAC 2 cut(s) 150, 495
SacI GAGCTC 2 cut(s) 399, 445
SacII CCGCGG 1 cut(s) 480
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 1 cut(s) 88
Sau3AI GATC 2 cut(s) 218, 246
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 4 cut(s) 195, 360, 399, 445
SetI ASST 6 cut(s) 214, 306, 399, 445, 450, 456
SfaNI GCATC 1 cut(s) 225
Sfr303I CCGCGG 1 cut(s) 480
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 1 cut(s) 337
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SsiI CCGC 4 cut(s) 88, 460, 477, 479
SspMI CTAG 2 cut(s) 305, 419
SstI GAGCTC 2 cut(s) 399, 445
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 2 cut(s) 51, 387
TaaI ACNGT 3 cut(s) 197, 225, 283
TaqI TCGA 2 cut(s) 215, 320
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 2 cut(s) 135, 383
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 4 cut(s) 159, 200, 228, 237
TspRI CASTG 4 cut(s) 159, 200, 228, 237
VpaK11BI GGWCC 1 cut(s) 337
XspI CTAG 2 cut(s) 305, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.