Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012255

Physical Location & Seq

Genomic Coordinates Forward (+)

35094495 .. 35098423

Gene Structure 3929 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1270500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGCATACGAAGACAATCCGTACCGTGATGAAGATGGGGAGCCATTGATGGACTTCAACGACTTTGGAGCTGGTCGCGACCCGTCTCCCGAGCCCCTCCAAGATGACGTCCTAGAAGATAACGACGATTGGCCCGACCACGACCGGTCTCGGACCCCCGTCCACGAGGATCCGTCGTCGTTGAAATCGAAGCCGAGGAAGCGGTTGATCAAGAAAAGCCAAGTGGGAAAATTGCCTGTGAGCTCTGATTTGGACGATGATGACGGCAATCGGAAGAGGTTGAAGAAGGAGAAGAGGCATAAGGGGGAGAAGAAGGGGTCTAAATACCCCAGGAGATCCTCATCTGACAAGGCGGCCACCGATGGTGAGGTCAAGGAGATGTGGGAAACCATCGCCGGCGGCGACTCTGAGGATGATCAAGAGGGTGTCAGGAATTACGACGACGACAACTTTATAGATGACACTGGTGTGCATCCTTCTGATCGGTATGGAAGTGACAACGAACCACGTTCTCCTAGTCACCATCCTCAGGCTGAAGAGGGTGAGGAAGATGATGAAATTAAGGATATGTTTAAGATGGGTAAGAAAAGGAAGAAGAATGAAAAAAGTCCCGCAGAAGTAGCATTCCTTGTTGAGAAAGTCATGGCTGAGCTCGAGGTAACAGCTGAAGAGGATGCAGATTTAAATAGACAGGGGAAACCTGCCATTAATAAGCTCAAGAAGTTGCCTCTTCTTACAGACGTCCTATCAAAGAAGCAGCTTCAACAAGAGTTTCTAGATCATGGAGTGTTAACTCTACTGAAGAATTGGCTTGAACCTCTTCCAGATGGAAGTTTACCGAACATAAATATACGTGCAGCAATTTTACAGATTTTGACTGATTTCCCTATAGATCTAGAGCAGTATGATAGAAGAGAACAACTGAAAAAGAGTGGCCTTGGAAAGGTCATCATGTTTTTATCAAAATCTGATGAAGAAACAACTTCCAACAGAAAACTTGCAAAGGAATTGGTTGACAAATGGAGTCGACCTATATTTAATAAAAGTACAAGGTTTGAGGATATGAGAAACGTTGACGATGAGAGAGTTCCCTTTAGAAGGCCTACAATTAAAAAGCCAGTGAGTAATGGCACAGGAATGGAATCTAGAGATGACGTGGATGAATTTTCAAGGGAAAGAAAATCTGGCCAATCATCTTCTCGGCAACATGCCTCAAGACCAGAAGCGACACCAATGGACTTTTTGGTGCGCCCACAATCCAGGATTGATCCTGATGAAATTAGAGCCCGTGCTAAACAAGGGACACAAGATCAGCGCCGTATGAAGATGAATAGGAAGTTACAGCAGTTGAAAGCACCAAAGAAGAAGCAGCTTCAGGCCACAAAATTAAGCGTGGAGGGTCGTGGCATGGTCAAGTATTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000414 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0010793 GO:0019219 GO:0019222 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032386 GO:0032784 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0033157 GO:0035065 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046822 GO:0046825 GO:0046831 GO:0050684 GO:0050789 GO:0050794 GO:0051049 GO:0051128 GO:0051171 GO:0051223 GO:0051246 GO:0051252 GO:0060255 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0080090 GO:0090087 GO:0090239 GO:1901983 GO:1902275 GO:1903311 GO:1903506 GO:1903827 GO:2000112 GO:2000197 GO:2000756 GO:2001141 GO:2001253

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med26

Protein Analysis

475

Amino Acids

54.42

Weight (kDa)

6.06

Isoelectric Point (pI)

54.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med26	PF08711	305 - 345	8.2e-13	TFIIS helical bundle-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32130 AT1G32130
fragaria_vesca	FvH4_5g23250
malus_domestica	MD06G1038400.v1.1 MD16G1270500.v1.1
prunus_persica	Prupe.5G026700_v2.0.a1 Prupe.5G026700_v2.0.a1
pyrus_communis	pycom06g03210 pycom16g23940
rosa_chinensis	RchiOBHm_Chr7g0212171
rosa_laevigata	RLG00000002920
rosa_multiflora	Rmu_sc0002283.1_g000053
rosa_roxburghii	Rroxscaffold_3G00247050
rosa_rugosa	Rorug07G0131800 Rorug07G0131900
rosa_samantha	Rh7AG264700 Rh7CG281200 Rh7DG271100
rosa_wichuraiana	Rw7G022600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	111, 744
Acc36I	ACCTGC	1 cut(s)	709
AccI	GTMKAC	1 cut(s)	1027
AccII	CGCG	1 cut(s)	78
AciI	CCGC	4 cut(s)	202, 353, 399, 612
AclI	AACGTT	1 cut(s)	1071
AclWI	GGATC	4 cut(s)	164, 177, 330, 1262
AcoI	YGGCCR	2 cut(s)	354, 1186
AcsI	RAATTY	1 cut(s)	1163
AcuI	CTGAAG	4 cut(s)	555, 687, 821, 1358
AcyI	GRCGYC	2 cut(s)	108, 741
AfaI	GTAC	2 cut(s)	23, 1048
AfiI	CCNNNNNNNGG	3 cut(s)	529, 943, 1098
AgeI	ACCGGT	1 cut(s)	144
AgsI	TTSAA	7 cut(s)	58, 184, 283, 764, 815, 1170, 1351
AjiI	CACGTC	1 cut(s)	1156
AjnI	CCWGG	2 cut(s)	329, 1259
AleI	CACNNNNGTG	1 cut(s)	467
AluBI	AGCT	7 cut(s)	71, 243, 652, 665, 715, 760, 1372
AluI	AGCT	7 cut(s)	71, 243, 652, 665, 715, 760, 1372
Alw21I	GWGCWC	2 cut(s)	245, 654
Alw26I	GTCTC	2 cut(s)	90, 153
AlwI	GGATC	4 cut(s)	164, 177, 330, 1262
Ama87I	CYCGRG	2 cut(s)	89, 653
AoxI	GGCC	6 cut(s)	131, 354, 934, 1100, 1186, 1377
ApeKI	GCWGC	3 cut(s)	757, 857, 1369
ApoI	RAATTY	1 cut(s)	1163
AseI	ATTAAT	1 cut(s)	708
AsiGI	ACCGGT	1 cut(s)	144
Asp700I	GAANNNNTTC	1 cut(s)	819
AspLEI	GCGC	2 cut(s)	1251, 1317
AspS9I	GGNCC	2 cut(s)	132, 153
AsuHPI	GGTGA	3 cut(s)	377, 512, 554
AvaI	CYCGRG	2 cut(s)	89, 653
AvaII	GGWCC	1 cut(s)	153
AxyI	CCTNAGG	1 cut(s)	528
BalI	TGGCCA	1 cut(s)	1188
BamHI	GGATCC	1 cut(s)	169
BanII	GRGCYC	4 cut(s)	96, 245, 654, 1288
BauI	CACGAG	1 cut(s)	164
BbsI	GAAGAC	1 cut(s)	18
Bbv12I	GWGCWC	2 cut(s)	245, 654
BbvI	GCAGC	3 cut(s)	769, 869, 1381
BccI	CCATC	7 cut(s)	29, 43, 356, 398, 531, 571, 821
BceAI	ACGGC	2 cut(s)	280, 1302
BciT130I	CCWGG	2 cut(s)	331, 1261
BclI	TGATCA	2 cut(s)	207, 415
BcoDI	GTCTC	2 cut(s)	90, 153
BfaI	CTAG	5 cut(s)	113, 516, 776, 896, 1146
BfmI	CTRYAG	1 cut(s)	888
BfoI	RGCGCY	1 cut(s)	1318
BfuAI	ACCTGC	1 cut(s)	709
BglII	AGATCT	1 cut(s)	892
BisI	GCNGC	5 cut(s)	354, 400, 758, 858, 1370
BlpI	GCTNAGC	1 cut(s)	648
BlsI	GCNGC	5 cut(s)	355, 401, 759, 859, 1371
Bme1390I	CCNGG	2 cut(s)	331, 1261
Bme18I	GGWCC	1 cut(s)	153
BmeT110I	CYCGRG	2 cut(s)	89, 653
BmgBI	CACGTC	1 cut(s)	1156
BmgT120I	GGNCC	2 cut(s)	132, 153
BmiI	GGNNCC	3 cut(s)	42, 155, 171
BmrFI	CCNGG	2 cut(s)	331, 1261
BmsI	GCATC	2 cut(s)	481, 664
BoxI	GACNNNNGTC	1 cut(s)	158
BpiI	GAAGAC	1 cut(s)	18
Bpu1102I	GCTNAGC	1 cut(s)	648
BpuEI	CTTGAG	2 cut(s)	701, 1198
BsaAI	YACGTR	1 cut(s)	854
BsaBI	GATNNNNATC	1 cut(s)	340
BsaHI	GRCGYC	2 cut(s)	108, 741
BsaI	GGTCTC	1 cut(s)	153
BsaJI	CCNNGG	3 cut(s)	194, 329, 937
BsaWI	WCCGGW	1 cut(s)	144
Bsc4I	CCNNNNNNNGG	3 cut(s)	529, 943, 1098
Bse118I	RCCGGY	2 cut(s)	144, 395
Bse1I	ACTGG	2 cut(s)	469, 1118
Bse21I	CCTNAGG	1 cut(s)	528
Bse8I	GATNNNNATC	1 cut(s)	340
BseBI	CCWGG	2 cut(s)	331, 1261
BseDI	CCNNGG	3 cut(s)	194, 329, 937
BseGI	GGATG	5 cut(s)	418, 472, 523, 679, 1165
BseJI	GATNNNNATC	1 cut(s)	340
BseLI	CCNNNNNNNGG	3 cut(s)	529, 943, 1098
BseMII	CTCAG	3 cut(s)	399, 542, 639
BseNI	ACTGG	2 cut(s)	469, 1118
BseXI	GCAGC	3 cut(s)	769, 869, 1381
BsgI	GTGCAG	1 cut(s)	876
Bsh1236I	CGCG	1 cut(s)	78
Bsh1285I	CGRYCG	1 cut(s)	145
BshFI	GGCC	6 cut(s)	133, 356, 936, 1102, 1188, 1379
BshTI	ACCGGT	1 cut(s)	144
BsiEI	CGRYCG	1 cut(s)	145
BsiHKAI	GWGCWC	2 cut(s)	245, 654
BsiHKCI	CYCGRG	2 cut(s)	89, 653
BsiSI	CCGG	2 cut(s)	145, 396
BslFI	GGGAC	2 cut(s)	594, 1315
BslI	CCNNNNNNNGG	3 cut(s)	529, 943, 1098
BsmAI	GTCTC	2 cut(s)	90, 153
BsmBI	CGTCTC	1 cut(s)	90
BsmFI	GGGAC	2 cut(s)	594, 1315
BsmI	GAATGC	1 cut(s)	623
BsnI	GGCC	6 cut(s)	133, 356, 936, 1102, 1188, 1379
Bso31I	GGTCTC	1 cut(s)	153
BsoBI	CYCGRG	2 cut(s)	89, 653
Bsp1286I	GDGCHC	4 cut(s)	96, 245, 654, 1288
Bsp143I	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
Bsp1720I	GCTNAGC	1 cut(s)	648
Bsp68I	TCGCGA	1 cut(s)	78
BspACI	CCGC	4 cut(s)	202, 353, 399, 612
BspANI	GGCC	6 cut(s)	133, 356, 936, 1102, 1188, 1379
BspCNI	CTCAG	3 cut(s)	400, 541, 640
BspFNI	CGCG	1 cut(s)	78
BspLI	GGNNCC	3 cut(s)	42, 155, 171
BspMI	ACCTGC	1 cut(s)	709
BspPI	GGATC	4 cut(s)	164, 177, 330, 1262
BspTNI	GGTCTC	1 cut(s)	153
BsrFI	RCCGGY	2 cut(s)	144, 395
BsrI	ACTGG	2 cut(s)	469, 1118
BssAI	RCCGGY	2 cut(s)	144, 395
BssECI	CCNNGG	3 cut(s)	194, 329, 937
BssMI	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
BssNI	GRCGYC	2 cut(s)	108, 741
BssSI	CACGAG	1 cut(s)	164
BssT1I	CCWWGG	1 cut(s)	937
Bst2BI	CACGAG	1 cut(s)	164
Bst2UI	CCWGG	2 cut(s)	331, 1261
Bst4CI	ACNGT	1 cut(s)	26
Bst6I	CTCTTC	7 cut(s)	269, 287, 531, 663, 735, 825, 907
BstACI	GRCGYC	2 cut(s)	108, 741
BstBAI	YACGTR	1 cut(s)	854
BstC8I	GCNNGC	1 cut(s)	397
BstDEI	CTNAG	3 cut(s)	408, 528, 648
BstENI	CCTNNNNNAGG	2 cut(s)	941, 1096
BstF5I	GGATG	5 cut(s)	418, 472, 523, 679, 1165
BstFNI	CGCG	1 cut(s)	78
BstH2I	RGCGCY	1 cut(s)	1318
BstHHI	GCGC	2 cut(s)	1251, 1317
BstKTI	GATC	9 cut(s)	172, 210, 338, 418, 484, 781, 895, 1270, 1312
BstMAI	GTCTC	2 cut(s)	90, 153
BstMBI	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
BstMCI	CGRYCG	1 cut(s)	145
BstMWI	GCNNNNNNNGC	2 cut(s)	199, 620
BstNI	CCWGG	2 cut(s)	331, 1261
BstNSI	RCATGY	1 cut(s)	1211
BstPAI	GACNNNNGTC	1 cut(s)	158
BstSCI	CCNGG	2 cut(s)	329, 1259
BstSFI	CTRYAG	1 cut(s)	888
BstUI	CGCG	1 cut(s)	78
BstV1I	GCAGC	3 cut(s)	769, 869, 1381
BstV2I	GAAGAC	1 cut(s)	18
BstX2I	RGATCY	3 cut(s)	169, 335, 892
BstYI	RGATCY	3 cut(s)	169, 335, 892
Bsu36I	CCTNAGG	1 cut(s)	528
BsuRI	GGCC	6 cut(s)	133, 356, 936, 1102, 1188, 1379
BtgZI	GCGATG	1 cut(s)	376
BtrI	CACGTC	1 cut(s)	1156
BtsCI	GGATG	5 cut(s)	418, 472, 523, 679, 1165
BtsIMutI	CAGTG	2 cut(s)	462, 1125
BtuMI	TCGCGA	1 cut(s)	78
BveI	ACCTGC	1 cut(s)	709
Cac8I	GCNNGC	1 cut(s)	397
CfoI	GCGC	2 cut(s)	1251, 1317
Cfr10I	RCCGGY	2 cut(s)	144, 395
Cfr13I	GGNCC	2 cut(s)	132, 153
Csp6I	GTAC	2 cut(s)	22, 1047
CspAI	ACCGGT	1 cut(s)	144
CviAII	CATG	5 cut(s)	643, 782, 952, 1208, 1408
CviQI	GTAC	2 cut(s)	22, 1047
DdeI	CTNAG	3 cut(s)	408, 528, 648
DpnI	GATC	9 cut(s)	171, 209, 337, 417, 483, 780, 894, 1269, 1311
DpnII	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
DraI	TTTAAA	1 cut(s)	684
EaeI	YGGCCR	2 cut(s)	354, 1186
Eam1104I	CTCTTC	7 cut(s)	269, 287, 531, 663, 735, 825, 907
EarI	CTCTTC	7 cut(s)	269, 287, 531, 663, 735, 825, 907
Ecl136II	GAGCTC	2 cut(s)	243, 652
Eco130I	CCWWGG	1 cut(s)	937
Eco147I	AGGCCT	1 cut(s)	1102
Eco24I	GRGCYC	4 cut(s)	96, 245, 654, 1288
Eco31I	GGTCTC	1 cut(s)	153
Eco47I	GGWCC	1 cut(s)	153
Eco53kI	GAGCTC	2 cut(s)	243, 652
Eco57I	CTGAAG	4 cut(s)	555, 687, 821, 1358
Eco81I	CCTNAGG	1 cut(s)	528
Eco88I	CYCGRG	2 cut(s)	89, 653
EcoICRI	GAGCTC	2 cut(s)	243, 652
EcoNI	CCTNNNNNAGG	2 cut(s)	941, 1096
EcoRII	CCWGG	2 cut(s)	329, 1259
EcoT14I	CCWWGG	1 cut(s)	937
EcoT38I	GRGCYC	4 cut(s)	96, 245, 654, 1288
ErhI	CCWWGG	1 cut(s)	937
Esp3I	CGTCTC	1 cut(s)	90
FaeI	CATG	5 cut(s)	646, 785, 955, 1211, 1411
FaqI	GGGAC	2 cut(s)	594, 1315
FatI	CATG	5 cut(s)	642, 781, 951, 1207, 1407
FauI	CCCGC	1 cut(s)	619
FbaI	TGATCA	2 cut(s)	207, 415
FblI	GTMKAC	1 cut(s)	1027
Fnu4HI	GCNGC	5 cut(s)	354, 400, 758, 858, 1370
FokI	GGATG	5 cut(s)	425, 459, 510, 686, 1172
FriOI	GRGCYC	4 cut(s)	96, 245, 654, 1288
Fsp4HI	GCNGC	5 cut(s)	354, 400, 758, 858, 1370
FspBI	CTAG	5 cut(s)	113, 516, 776, 896, 1146
GlaI	GCGC	2 cut(s)	1250, 1316
GluI	GCNGC	5 cut(s)	354, 400, 758, 858, 1370
HaeII	RGCGCY	1 cut(s)	1318
HaeIII	GGCC	6 cut(s)	133, 356, 936, 1102, 1188, 1379
HapII	CCGG	2 cut(s)	145, 396
HhaI	GCGC	2 cut(s)	1251, 1317
Hin1I	GRCGYC	2 cut(s)	108, 741
Hin1II	CATG	5 cut(s)	646, 785, 955, 1211, 1411
Hin6I	GCGC	2 cut(s)	1249, 1315
HinP1I	GCGC	2 cut(s)	1249, 1315
HincII	GTYRAC	4 cut(s)	792, 1015, 1028, 1075
HindII	GTYRAC	4 cut(s)	792, 1015, 1028, 1075
HinfI	GANTC	3 cut(s)	404, 1024, 1142
HpaI	GTTAAC	1 cut(s)	792
HpaII	CCGG	2 cut(s)	145, 396
HphI	GGTGA	3 cut(s)	377, 512, 554
Hpy166II	GTNNAC	6 cut(s)	163, 792, 836, 1015, 1028, 1075
Hpy188I	TCNGA	7 cut(s)	153, 247, 273, 346, 409, 481, 970
Hpy8I	GTNNAC	6 cut(s)	163, 792, 836, 1015, 1028, 1075
Hpy99I	CGWCG	5 cut(s)	128, 178, 181, 443, 446
HpyAV	CCTTC	4 cut(s)	280, 307, 486, 1092
HpyCH4III	ACNGT	1 cut(s)	26
HpyCH4IV	ACGT	6 cut(s)	108, 508, 741, 853, 1071, 1155
HpyCH4V	TGCA	4 cut(s)	472, 677, 857, 1001
HpyF10VI	GCNNNNNNNGC	2 cut(s)	199, 620
HpyF3I	CTNAG	3 cut(s)	408, 528, 648
HpySE526I	ACGT	6 cut(s)	108, 508, 741, 853, 1071, 1155
Hsp92I	GRCGYC	2 cut(s)	108, 741
Hsp92II	CATG	5 cut(s)	646, 785, 955, 1211, 1411
HspAI	GCGC	2 cut(s)	1249, 1315
KroI	GCCGGC	1 cut(s)	395
KroNI	GCCGGC	1 cut(s)	397
Ksp22I	TGATCA	2 cut(s)	207, 415
KspAI	GTTAAC	1 cut(s)	792
Kzo9I	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
LmnI	GCTCC	2 cut(s)	40, 68
Lsp1109I	GCAGC	3 cut(s)	769, 869, 1381
LweI	GCATC	2 cut(s)	481, 664
MaeI	CTAG	5 cut(s)	113, 516, 776, 896, 1146
MaeII	ACGT	6 cut(s)	108, 508, 741, 853, 1071, 1155
MaeIII	GTNAC	4 cut(s)	494, 518, 658, 1338
MalI	GATC	9 cut(s)	171, 209, 337, 417, 483, 780, 894, 1269, 1311
MboI	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
MflI	RGATCY	3 cut(s)	169, 335, 892
MhlI	GDGCHC	4 cut(s)	96, 245, 654, 1288
MlsI	TGGCCA	1 cut(s)	1188
MluNI	TGGCCA	1 cut(s)	1188
MlyI	GAGTC	2 cut(s)	398, 1033
MmeI	TCCRAC	1 cut(s)	1011
Mox20I	TGGCCA	1 cut(s)	1188
MreI	CGCCGGCG	1 cut(s)	395
MroNI	GCCGGC	1 cut(s)	395
MroXI	GAANNNNTTC	1 cut(s)	819
MscI	TGGCCA	1 cut(s)	1188
MseI	TTAA	8 cut(s)	561, 573, 683, 708, 791, 1038, 1110, 1388
MslI	CAYNNNNRTG	2 cut(s)	467, 1136
Msp20I	TGGCCA	1 cut(s)	1188
MspA1I	CMGCKG	1 cut(s)	665
MspI	CCGG	2 cut(s)	145, 396
MspR9I	CCNGG	2 cut(s)	331, 1261
Mva1269I	GAATGC	1 cut(s)	623
MvaI	CCWGG	2 cut(s)	331, 1261
MvnI	CGCG	1 cut(s)	78
MwoI	GCNNNNNNNGC	2 cut(s)	199, 620
NaeI	GCCGGC	1 cut(s)	397
NdeII	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
NgoMIV	GCCGGC	1 cut(s)	395
NlaIII	CATG	5 cut(s)	646, 785, 955, 1211, 1411
NlaIV	GGNNCC	3 cut(s)	42, 155, 171
NmeAIII	GCCGAG	2 cut(s)	219, 1180
NmuCI	GTSAC	2 cut(s)	494, 518
NruI	TCGCGA	1 cut(s)	78
NspI	RCATGY	1 cut(s)	1211
OliI	CACNNNNGTG	1 cut(s)	467
PaeR7I	CTCGAG	1 cut(s)	653
PceI	AGGCCT	1 cut(s)	1102
PcsI	WCGNNNNNNNCGW	3 cut(s)	132, 185, 399
PctI	GAATGC	1 cut(s)	623
PdiI	GCCGGC	1 cut(s)	397
PdmI	GAANNNNTTC	1 cut(s)	819
PfeI	GAWTC	1 cut(s)	1142
PfoI	TCCNGGA	1 cut(s)	1259
PinAI	ACCGGT	1 cut(s)	144
PkrI	GCNGC	5 cut(s)	355, 401, 759, 859, 1371
PleI	GAGTC	2 cut(s)	398, 1032
PpsI	GAGTC	2 cut(s)	398, 1032
Ppu21I	YACGTR	1 cut(s)	854
PshAI	GACNNNNGTC	1 cut(s)	158
PshBI	ATTAAT	1 cut(s)	708
Psp124BI	GAGCTC	2 cut(s)	245, 654
Psp1406I	AACGTT	1 cut(s)	1071
Psp6I	CCWGG	2 cut(s)	329, 1259
PspGI	CCWGG	2 cut(s)	329, 1259
PspN4I	GGNNCC	3 cut(s)	42, 155, 171
PspPI	GGNCC	2 cut(s)	132, 153
PspXI	VCTCGAGB	1 cut(s)	653
PsuI	RGATCY	3 cut(s)	169, 335, 892
PvuII	CAGCTG	1 cut(s)	665
RruI	TCGCGA	1 cut(s)	78
RsaI	GTAC	2 cut(s)	23, 1048
RsaNI	GTAC	2 cut(s)	22, 1047
RseI	CAYNNNNRTG	2 cut(s)	467, 1136
SacI	GAGCTC	2 cut(s)	245, 654
SalI	GTCGAC	1 cut(s)	1026
SaqAI	TTAA	8 cut(s)	561, 573, 683, 708, 791, 1038, 1110, 1388
SatI	GCNGC	5 cut(s)	354, 400, 758, 858, 1370
Sau3AI	GATC	9 cut(s)	169, 207, 335, 415, 481, 778, 892, 1267, 1309
Sau96I	GGNCC	2 cut(s)	132, 153
SchI	GAGTC	2 cut(s)	398, 1033
ScrFI	CCNGG	2 cut(s)	331, 1261
SduI	GDGCHC	4 cut(s)	96, 245, 654, 1288
SfaNI	GCATC	2 cut(s)	481, 664
SfcI	CTRYAG	1 cut(s)	888
Sfr274I	CTCGAG	1 cut(s)	653
SgrAI	CRCCGGYG	1 cut(s)	395
SinI	GGWCC	1 cut(s)	153
SlaI	CTCGAG	1 cut(s)	653
SmiI	ATTTAAAT	1 cut(s)	684
SmiMI	CAYNNNNRTG	2 cut(s)	467, 1136
SmlI	CTYRAG	3 cut(s)	653, 716, 1213
SmoI	CTYRAG	3 cut(s)	653, 716, 1213
SseBI	AGGCCT	1 cut(s)	1102
SsiI	CCGC	4 cut(s)	202, 353, 399, 612
SspMI	CTAG	5 cut(s)	113, 516, 776, 896, 1146
SstI	GAGCTC	2 cut(s)	245, 654
StuI	AGGCCT	1 cut(s)	1102
StyD4I	CCNGG	2 cut(s)	329, 1259
StyI	CCWWGG	1 cut(s)	937
SwaI	ATTTAAAT	1 cut(s)	684
TaaI	ACNGT	1 cut(s)	26
TaiI	ACGT	6 cut(s)	111, 511, 744, 856, 1074, 1158
TaqI	TCGA	3 cut(s)	188, 654, 1027
TatI	WGTACW	1 cut(s)	1046
TauI	GCSGC	2 cut(s)	356, 402
TfiI	GAWTC	1 cut(s)	1142
Tru1I	TTAA	8 cut(s)	561, 573, 683, 708, 791, 1038, 1110, 1388
Tru9I	TTAA	8 cut(s)	561, 573, 683, 708, 791, 1038, 1110, 1388
TscAI	CASTG	2 cut(s)	469, 1125
TseFI	GTSAC	2 cut(s)	494, 518
TseI	GCWGC	3 cut(s)	757, 857, 1369
Tsp45I	GTSAC	2 cut(s)	494, 518
TspDTI	ATGAA	8 cut(s)	45, 570, 615, 987, 1176, 1290, 1337, 1343
TspGWI	ACGGA	2 cut(s)	9, 162
TspRI	CASTG	2 cut(s)	469, 1125
VpaK11BI	GGWCC	1 cut(s)	153
VspI	ATTAAT	1 cut(s)	708
XagI	CCTNNNNNAGG	2 cut(s)	941, 1096
XapI	RAATTY	1 cut(s)	1163
XbaI	TCTAGA	3 cut(s)	775, 895, 1145
XceI	RCATGY	1 cut(s)	1211
XhoI	CTCGAG	1 cut(s)	653
XmiI	GTMKAC	1 cut(s)	1027
XmnI	GAANNNNTTC	1 cut(s)	819
XspI	CTAG	5 cut(s)	113, 516, 776, 896, 1146
ZraI	GACGTC	2 cut(s)	109, 742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.