MD16G1285000.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013788
Physical Location & Seq
Forward (+)
40554436 .. 40562902
8467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1285000.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGGGAAACTGTTACGGATCTCCAGTGACTAATCATAGCCCATCATCTACAACCAAGCTGCCTACCTCAGTAGCATCGAGATACGATAACAAGAAAAATGGATTCGTTGGAAAAAGTGTGACCAGCACCACAAAAGTTGCAAGGAATATTAGTGGCAAAAGAAATACTGGTGGAACAACGGAGAGAAGTGAAAGCAAGGCACAAGTGATATTAAGCAAAACCATCAGCAGAAATAATTATGCTGAAGCAGCTGGAGGTGTGGCTCCTCCTCCTCCTGATGCGGCAGCTACAGCTGCTCCAATCGAAAGTATACCAAAGTTGAAAGAGTTCACGTTAGCAGAGCTGAAGAAGTGTACTCAAAATTTCCGACCAGATACAATACTAGGAGAGGGAGGTTTCGGAAGGGTTTTCAAGGGCTGGGTCGATGCCTCAACGTATGCCCCATCAAAGGGAGGCGTCGGCTTGGCCGTCGCAGTCAAGAAGTCCAGCTCAGATAGCTATCAAGGTCACCAAGAATGGCAGGCGGAGGTGAATTTCTTGGGGAAGTGTCGTCATCCAAACCTCGTTAGGCTGCTGGGACATTGCTGTGAAGAAAACCAATTCCTTCTCGTCTACGAATATATGCAGAAAGGCAGCTTGGAAAACCACCTTTTCAGAAAAGGTCAAGAACCACTTCCATGGGAAATAAGACTAAAGATAGCAATTGGAGCTGCACGAGGCCTTGCTTATCTGCACAACTCAGAAAAAGTCATTTACCGTGACTTTAAGGCCTCCAATATTTTGCTGGATCCGGCCTACAATGCGAAACTTTCAGATTTTGGACTGGTAAAGATGGGGCCTATGAATGGAAACTCCCACGTAACCACACGTATTATTGGAACTTACGGATACGCAGCTCCAGAATATGTCGCAACTGGGCACTTGTACGTAAAGAGCGATGTGTACGGTTTCGGCGTTGTATTGCTGGAGATGCTAACGGGATTACGGGCACTTGACAGTAATCGGCCATCTCGCGAGCACAGTTTGGTAGACTGGGTGAGTCCGTTGCTTCACAAGAAAAAGGAGCTGAAGAAGATAATGGACCCAGGGCTTGGAGATGAATACCCCTTAAAAGGTGCCTGCCAAGCAGCTGAGCTTATTTTGAAATGCCTAGAACCTGATCCTAAAATCAGACCATCCATGCAAGAGGTTTTGATAGTTCTGGAAAAGATAAACGACATTAAGGATCAGAAACCCAAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

415

Amino Acids

45.36

Weight (kDa)

9.28

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 125 - 398 7.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 126 - 398 4.1e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013788)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76360
fragaria_vesca FvH4_5g25310
malus_domestica MD06G1009600.v1.1 MD16G1285000.v1.1
prunus_persica Prupe.5G010900_v2.0.a1
pyrus_communis pycom06g00790 pycom16g25930
rosa_chinensis RchiOBHm_Chr7g0215531
rosa_laevigata RLG00000002650
rosa_multiflora Rmu_sc0000898.1_g000098
rosa_roxburghii Rroxscaffold_3G00244160
rosa_rugosa Rorug07G0154700
rosa_samantha Rh7AG290800 Rh7BG282900 Rh7CG310700 Rh7DG294400
rosa_wichuraiana Rw7G024810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1115
AccB7I CCANNNNNTGG 1 cut(s) 1091
AccI GTMKAC 3 cut(s) 310, 612, 1029
AccII CGCG 1 cut(s) 1014
AciI CCGC 2 cut(s) 281, 524
AclWI GGATC 5 cut(s) 25, 782, 795, 1154, 1233
AcoI YGGCCR 2 cut(s) 465, 1004
AcsI RAATTY 2 cut(s) 361, 532
AcuI CTGAAG 3 cut(s) 264, 365, 1088
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 3 cut(s) 355, 926, 944
AfiI CCNNNNNNNGG 5 cut(s) 448, 449, 845, 1091, 1112
AflIII ACRYGT 1 cut(s) 866
AgsI TTSAA 3 cut(s) 322, 412, 1144
AjnI CCWGG 1 cut(s) 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
Alw21I GWGCWC 1 cut(s) 1020
AlwI GGATC 5 cut(s) 25, 782, 795, 1154, 1233
AoxI GGCC 6 cut(s) 465, 718, 768, 792, 836, 1004
ApeKI GCWGC 9 cut(s) 58, 248, 284, 293, 571, 633, 710, 893, 1127
ApoI RAATTY 2 cut(s) 361, 532
Asp700I GAANNNNTTC 2 cut(s) 326, 672
AspS9I GGNCC 2 cut(s) 836, 1081
AsuHPI GGTGA 3 cut(s) 500, 541, 1048
AvaII GGWCC 1 cut(s) 1081
BaeGI GKGCMC 2 cut(s) 921, 991
BamHI GGATCC 1 cut(s) 787
BanI GGYRCC 1 cut(s) 1115
BauI CACGAG 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 9 cut(s) 45, 260, 280, 296, 558, 645, 697, 905, 1139
BccI CCATC 6 cut(s) 49, 230, 451, 826, 1015, 1183
BceAI ACGGC 1 cut(s) 452
BciT130I CCWGG 1 cut(s) 1086
BciVI GTATCC 1 cut(s) 881
BfaI CTAG 3 cut(s) 383, 1151, 1243
BfmI CTRYAG 1 cut(s) 288
BfuI GTATCC 1 cut(s) 881
BlpI GCTNAGC 1 cut(s) 1131
Bme1390I CCNGG 1 cut(s) 1086
Bme18I GGWCC 1 cut(s) 1081
BmgT120I GGNCC 2 cut(s) 836, 1081
BmiI GGNNCC 5 cut(s) 264, 789, 837, 1083, 1117
BmrFI CCNGG 1 cut(s) 1086
BmrI ACTGGG 2 cut(s) 924, 1042
BmsI GCATC 4 cut(s) 83, 268, 415, 960
BmuI ACTGGG 2 cut(s) 924, 1042
BpmI CTGGAG 4 cut(s) 6, 273, 882, 986
Bpu1102I GCTNAGC 1 cut(s) 1131
BsaAI YACGTR 3 cut(s) 859, 869, 928
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 677, 1084, 1085
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 5 cut(s) 448, 449, 845, 1091, 1112
Bse1I ACTGG 5 cut(s) 23, 172, 828, 919, 1037
Bse3DI GCAATG 1 cut(s) 580
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 1 cut(s) 1086
BseDI CCNNGG 3 cut(s) 677, 1084, 1085
BseGI GGATG 2 cut(s) 553, 1175
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 5 cut(s) 448, 449, 845, 1091, 1112
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 4 cut(s) 81, 504, 753, 1122
BseNI ACTGG 5 cut(s) 23, 172, 828, 919, 1037
BseRI GAGGAG 3 cut(s) 255, 258, 261
BseSI GKGCMC 2 cut(s) 921, 991
BseXI GCAGC 9 cut(s) 45, 260, 280, 296, 558, 645, 697, 905, 1139
BseYI CCCAGC 2 cut(s) 417, 574
BsgI GTGCAG 2 cut(s) 696, 716
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 6 cut(s) 467, 720, 770, 794, 838, 1006
BshNI GGYRCC 1 cut(s) 1115
BsiHKAI GWGCWC 1 cut(s) 1020
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 5 cut(s) 448, 449, 845, 1091, 1112
BsmFI GGGAC 1 cut(s) 591
BsnI GGCC 6 cut(s) 467, 720, 770, 794, 838, 1006
Bsp1286I GDGCHC 3 cut(s) 921, 991, 1020
Bsp143I GATC 4 cut(s) 17, 787, 1159, 1225
Bsp1720I GCTNAGC 1 cut(s) 1131
Bsp19I CCATGG 1 cut(s) 677
Bsp68I TCGCGA 1 cut(s) 1014
BspACI CCGC 2 cut(s) 281, 524
BspANI GGCC 6 cut(s) 467, 720, 770, 794, 838, 1006
BspCNI CTCAG 4 cut(s) 80, 503, 752, 1123
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 5 cut(s) 264, 789, 837, 1083, 1117
BspPI GGATC 5 cut(s) 25, 782, 795, 1154, 1233
BspT107I GGYRCC 1 cut(s) 1115
BsrDI GCAATG 1 cut(s) 580
BsrI ACTGG 5 cut(s) 23, 172, 828, 919, 1037
BssECI CCNNGG 3 cut(s) 677, 1084, 1085
BssMI GATC 4 cut(s) 17, 787, 1159, 1225
BssNAI GTATAC 1 cut(s) 311
BssNI GRCGYC 1 cut(s) 456
BssSI CACGAG 1 cut(s) 714
BssT1I CCWWGG 1 cut(s) 677
Bst1107I GTATAC 1 cut(s) 311
Bst2BI CACGAG 1 cut(s) 714
Bst2UI CCWGG 1 cut(s) 1086
Bst4CI ACNGT 5 cut(s) 11, 758, 947, 998, 1022
BstACI GRCGYC 1 cut(s) 456
BstBAI YACGTR 3 cut(s) 859, 869, 928
BstC8I GCNNGC 3 cut(s) 522, 1016, 1120
BstDEI CTNAG 4 cut(s) 67, 490, 739, 1131
BstDSI CCRYGG 1 cut(s) 677
BstEII GGTNACC 1 cut(s) 506
BstF5I GGATG 2 cut(s) 553, 1175
BstFNI CGCG 1 cut(s) 1014
BstKTI GATC 4 cut(s) 20, 790, 1162, 1228
BstMBI GATC 4 cut(s) 17, 787, 1159, 1225
BstMWI GCNNNNNNNGC 8 cut(s) 248, 290, 293, 495, 707, 800, 970, 1124
BstNI CCWGG 1 cut(s) 1086
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 1084
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 2 cut(s) 921, 991
BstSNI TACGTA 1 cut(s) 928
BstUI CGCG 1 cut(s) 1014
BstV1I GCAGC 9 cut(s) 45, 260, 280, 296, 558, 645, 697, 905, 1139
BstX2I RGATCY 2 cut(s) 17, 787
BstXI CCANNNNNNTGG 1 cut(s) 678
BstYI RGATCY 2 cut(s) 17, 787
BstZ17I GTATAC 1 cut(s) 311
BsuI GTATCC 1 cut(s) 881
BsuRI GGCC 6 cut(s) 467, 720, 770, 794, 838, 1006
BtgI CCRYGG 1 cut(s) 677
BtgZI GCGATG 1 cut(s) 951
BtsCI GGATG 2 cut(s) 553, 1175
BtsIMutI CAGTG 1 cut(s) 30
BtuMI TCGCGA 1 cut(s) 1014
Cac8I GCNNGC 3 cut(s) 522, 1016, 1120
Cfr13I GGNCC 2 cut(s) 836, 1081
CseI GACGC 1 cut(s) 445
Csp6I GTAC 3 cut(s) 354, 925, 943
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 2 cut(s) 678, 1180
CviQI GTAC 3 cut(s) 354, 925, 943
DdeI CTNAG 4 cut(s) 67, 490, 739, 1131
DpnI GATC 4 cut(s) 19, 789, 1161, 1227
DpnII GATC 4 cut(s) 17, 787, 1159, 1225
EaeI YGGCCR 2 cut(s) 465, 1004
EciI GGCGGA 1 cut(s) 539
Eco105I TACGTA 1 cut(s) 928
Eco130I CCWWGG 1 cut(s) 677
Eco147I AGGCCT 2 cut(s) 720, 770
Eco47I GGWCC 1 cut(s) 1081
Eco57I CTGAAG 3 cut(s) 264, 365, 1088
Eco91I GGTNACC 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 836
EcoO65I GGTNACC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 1084
EcoT14I CCWWGG 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 677
FaeI CATG 2 cut(s) 681, 1183
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 591
FatI CATG 2 cut(s) 677, 1179
FblI GTMKAC 3 cut(s) 310, 612, 1029
FokI GGATG 2 cut(s) 540, 1162
FspBI CTAG 3 cut(s) 383, 1151, 1243
GsaI CCCAGC 2 cut(s) 421, 578
GsuI CTGGAG 4 cut(s) 6, 273, 882, 986
HaeIII GGCC 6 cut(s) 467, 720, 770, 794, 838, 1006
HapII CCGG 1 cut(s) 791
HgaI GACGC 1 cut(s) 445
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 2 cut(s) 681, 1183
HinfI GANTC 2 cut(s) 102, 1039
HpaII CCGG 1 cut(s) 791
HphI GGTGA 3 cut(s) 500, 541, 1048
Hpy166II GTNNAC 6 cut(s) 311, 330, 354, 613, 943, 1030
Hpy188I TCNGA 8 cut(s) 368, 401, 493, 656, 742, 814, 1172, 1230
Hpy188III TCNNGA 7 cut(s) 78, 275, 478, 665, 899, 1013, 1202
Hpy8I GTNNAC 6 cut(s) 311, 330, 354, 613, 943, 1030
Hpy99I CGWCG 2 cut(s) 461, 473
HpyAV CCTTC 2 cut(s) 396, 614
HpyCH4III ACNGT 5 cut(s) 11, 758, 947, 998, 1022
HpyCH4IV ACGT 5 cut(s) 332, 434, 858, 868, 927
HpyCH4V TGCA 5 cut(s) 140, 625, 713, 733, 1183
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 290, 293, 495, 707, 800, 970, 1124
HpyF3I CTNAG 4 cut(s) 67, 490, 739, 1131
HpySE526I ACGT 5 cut(s) 332, 434, 858, 868, 927
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 2 cut(s) 681, 1183
Kzo9I GATC 4 cut(s) 17, 787, 1159, 1225
LmnI GCTCC 5 cut(s) 268, 301, 707, 901, 1063
Lsp1109I GCAGC 9 cut(s) 45, 260, 280, 296, 558, 645, 697, 905, 1139
LweI GCATC 4 cut(s) 83, 268, 415, 960
MaeI CTAG 3 cut(s) 383, 1151, 1243
MaeII ACGT 5 cut(s) 332, 434, 858, 868, 927
MaeIII GTNAC 6 cut(s) 11, 25, 118, 506, 758, 859
MalI GATC 4 cut(s) 19, 789, 1161, 1227
MboI GATC 4 cut(s) 17, 787, 1159, 1225
MboII GAAGA 4 cut(s) 358, 602, 1081, 1084
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 2 cut(s) 17, 787
MhlI GDGCHC 3 cut(s) 921, 991, 1020
MluCI AATT 5 cut(s) 235, 361, 532, 599, 702
MlyI GAGTC 1 cut(s) 1048
MmeI TCCRAC 2 cut(s) 88, 391
MroXI GAANNNNTTC 2 cut(s) 326, 672
MseI TTAA 4 cut(s) 212, 765, 1109, 1221
MslI CAYNNNNRTG 2 cut(s) 585, 676
MspA1I CMGCKG 3 cut(s) 251, 293, 1130
MspI CCGG 1 cut(s) 791
MspR9I CCNGG 1 cut(s) 1086
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 1 cut(s) 1086
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 8 cut(s) 248, 290, 293, 495, 707, 800, 970, 1124
NcoI CCATGG 1 cut(s) 677
NdeII GATC 4 cut(s) 17, 787, 1159, 1225
NlaIII CATG 2 cut(s) 681, 1183
NlaIV GGNNCC 5 cut(s) 264, 789, 837, 1083, 1117
NmuCI GTSAC 4 cut(s) 25, 118, 506, 758
NruI TCGCGA 1 cut(s) 1014
PasI CCCWGGG 1 cut(s) 1085
PceI AGGCCT 2 cut(s) 720, 770
PcsI WCGNNNNNNNCGW 3 cut(s) 465, 933, 951
PdmI GAANNNNTTC 2 cut(s) 326, 672
PfeI GAWTC 1 cut(s) 102
PflMI CCANNNNNTGG 1 cut(s) 1091
PleI GAGTC 1 cut(s) 1047
PpsI GAGTC 1 cut(s) 1047
Ppu21I YACGTR 3 cut(s) 859, 869, 928
Psp6I CCWGG 1 cut(s) 1084
PspEI GGTNACC 1 cut(s) 506
PspFI CCCAGC 2 cut(s) 417, 574
PspGI CCWGG 1 cut(s) 1084
PspN4I GGNNCC 5 cut(s) 264, 789, 837, 1083, 1117
PspPI GGNCC 2 cut(s) 836, 1081
PsuI RGATCY 2 cut(s) 17, 787
PvuII CAGCTG 3 cut(s) 251, 293, 1130
RruI TCGCGA 1 cut(s) 1014
RsaI GTAC 3 cut(s) 355, 926, 944
RsaNI GTAC 3 cut(s) 354, 925, 943
RseI CAYNNNNRTG 2 cut(s) 585, 676
SaqAI TTAA 4 cut(s) 212, 765, 1109, 1221
Sau3AI GATC 4 cut(s) 17, 787, 1159, 1225
Sau96I GGNCC 2 cut(s) 836, 1081
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 1 cut(s) 1086
SduI GDGCHC 3 cut(s) 921, 991, 1020
SfaNI GCATC 4 cut(s) 83, 268, 415, 960
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 1 cut(s) 1081
SmiMI CAYNNNNRTG 2 cut(s) 585, 676
SnaBI TACGTA 1 cut(s) 928
Sse9I AATT 5 cut(s) 235, 361, 532, 599, 702
SseBI AGGCCT 2 cut(s) 720, 770
SsiI CCGC 2 cut(s) 281, 524
SspI AATATT 2 cut(s) 148, 778
SspMI CTAG 3 cut(s) 383, 1151, 1243
StuI AGGCCT 2 cut(s) 720, 770
StyD4I CCNGG 1 cut(s) 1084
StyI CCWWGG 1 cut(s) 677
TaaI ACNGT 5 cut(s) 11, 758, 947, 998, 1022
TaiI ACGT 5 cut(s) 335, 437, 861, 871, 930
TaqI TCGA 3 cut(s) 77, 303, 423
TasI AATT 5 cut(s) 235, 361, 532, 599, 702
TatI WGTACW 1 cut(s) 353
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 4 cut(s) 212, 765, 1109, 1221
Tru9I TTAA 4 cut(s) 212, 765, 1109, 1221
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 4 cut(s) 25, 118, 506, 758
TseI GCWGC 9 cut(s) 58, 248, 284, 293, 571, 633, 710, 893, 1127
Tsp45I GTSAC 4 cut(s) 25, 118, 506, 758
TspDTI ATGAA 2 cut(s) 857, 1113
TspGWI ACGGA 4 cut(s) 30, 194, 900, 1032
TspRI CASTG 1 cut(s) 30
Van91I CCANNNNNTGG 1 cut(s) 1091
VpaK11BI GGWCC 1 cut(s) 1081
XapI RAATTY 2 cut(s) 361, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XmiI GTMKAC 3 cut(s) 310, 612, 1029
XmnI GAANNNNTTC 2 cut(s) 326, 672
XspI CTAG 3 cut(s) 383, 1151, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.