Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008014

Physical Location & Seq

Genomic Coordinates Reverse (-)

436648 .. 442462

Gene Structure 5815 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1006400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGGCGGCTGATCAGCGGAGAAAGCGACTAAATGGTGTTATCATTGCCGGTTGCAATTCCAGGGAGCAACATAAAGCAAAAAAGAAAAGTATTAGATTGCTTCAGGATGATTCAAACATAAAACCACATATTTCTCTTGAGTGGGATGGGAATCAGAATATGGTAGTTGCCAAAAGGGATCAAATTGGCATAAGTAGGAGAACTTTGAGGCCGTTCGTTGATTCCGCTTTCAATAGCCACAATACTTTGGCAGATGTTTTTGCTGTTCCTGAGGGAATCTATGATTTGGAAAATTTGGAAGATGTGCTTTCATATGAGGTTTGGAATACTCATCTTTCTGAGATTGAGAAGAATCACCTTATGCAGTTTCTTCCTAGCAGAGCAGAGGCAGAGGAAGTTGTGCAAGCATTGCTTGCTGGGGGTTGCTTTGACTTTGGAAGCCCTTTTCTTAAATGGGGTGCTTCACTTTGCTCAGGTGATTTCCATCCTGATGCAATACTTCGTAGGGAACAATGTCTAAAGAATGATAAGAAAACATTCTACAATGAGTTGCAGGTGTATCATAATGATATGATTGCATATTTACAGAAGTTGAAGAAGAGATGCGCAAGCTGTAAGGATCCTGAACAGGAAATTGTGCAGAATATGTGGAGGTTAAGAAGTGATGTTGAGAAACAATTTTTTTCACATGCAAATGAATCCAGATCGCATGATCTTGAGGAAAATGCCATAGTTACATCTGAATCTTGTTCTTGGGAGAAAGCATGCAGTAGTGATAACCAGATTTCCTCAGTGAACAAAGGAGGAGATTTTCAGAACAGGATCCGTGAGAAAAGCTTCCTAAAGGACAAAGGTAGAAATTTATTGGTTACACCAGATGGGGCACATAATGCGGGAGGAAGATCCAAAAAAGGAGACAATCTACACAAGCGCAATATCTGTAGTAGCGATGGTGCTAAATACATGTCATATGTTAAGATTAGCACGAAGCAGTACGAAATTGTTAAGAGCCTGAAGCAGTCTGGCAAAAGCATTCAGTCCAGGTCTCTCAACCGGATATTAGGTAACCTTGATAGCTTTGAGGTACAACCATATGAAGTCTTTGTGGAAGAAGAACAGAAGAAGTTGCATCAGCACTGGTTGCAATTGGCAATTAAAGATATCCCGGCAGCTTATGCAAACTGGATTGAAATGCATTTACAAAGACGGCAAATGACAAACTCTTTGGAGAAGGACATGAAAAGGTGGCTAAAGCCCCTGATAGAGGATGACAAAGGTGATCACAACCTTGAAAGCGTGCTTCAAGATAAGATAGCTATTGGAGCAACAAAGCATCAATCTCCCATGGAAGATGATGAAAAGTCTGTACCCAGTTTCCCACAGGGTGATGAGTGCAACCCCACAGACATGGAAGAAGATGACTATTCTCGAGAGAAGCTTACTGGCAGTCGTGAGTGCAACCGCACAGATATAGATTCAGATGAACATTTCAGTACAGAGTCAGATAATGATTCAGAAAAGCATATCATTATAGAATCAGATCATTCTCCTCCAAATGTACCTGATTATGCGGAGTATCTGAGTACTGCAGATACTGCGGGAGCACAATTATGTGCTAGTGGAGATGCCTGGACGGCAGTTAATGTGCCACATTCTTACAAGGATTCTACCACAAGTCATGCATATTCATCTGCCAGTGAATTATCGCTCGCACATCTACAAGTTAATGAAGTTCAACAGACACACTTAGTTGCTATGGAGTCTGACTTGCCTGTAGGAGACAGTGGGAAAGATTTATTGCACATACAATCAGAGAATGGTTCCTTCAGTTCTTACCATAACCAAGATCGAAATGAATTGCTTCAATCTCTCTTTAAGGGCCAGAGCATGCTATCTTACAATCCTGAGAAGAAACAGACAGGGTTGGATTTCCGGACACCAGCCAATGTGTTTATGGGTCAGTTTCCAGTGCATTTTGAAGAGCAGCAACACCAGTCACTGCCTCTGGAGCAAGGCCAGAAGAGAGAGAGCGAGGTTTACATGCAACCAAGATTATCTGAGAACGTTTACTCTGATGGAGGTAGATACTTAAATTCGAGGCCAGAACATCCGGCCCCAGTCGATGTGCAGGATTGGGCTGTCAATAGTGTTCGCATGCCAAGACCTCTACAATCTCACTTAGATGGTGGAGAAATGTTGAGTCATAATTGGTTTTCTGGTGAGCATCAAGTGCATGGTGGATGGTCGGCGTCAGGTGGTACTAGTGTCATGAGTCAGATCATTGCGAATGGAACGAATGCAGATCAAAGCTTATTCAGTGTTCTATCCCATTGTAACCAATTGCGATCAAGTAGCCCCTATCAGCACGTAGCCTCCACCGAGCAGTTCATTTCCTCTAGGAGCTATGGAACTGTGGGTGGGGCCACTCCAAGGATCGGAAATGTTGTACCACAAGCAGGTCATGAACTTGATTACTTGGGTGGGCGGGAAGCAGCTACTCCCATGATGCACGATGATATGCAATGGATGAATTTACCGCGTCAGAACTCTGGTTTACGTGATCCTATGGGAAAACCATTCTTGAGGTCGTGGAACCCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

867

Amino Acids

97.84

Weight (kDa)

5.71

Isoelectric Point (pI)

61.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008014)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13950 AT5G13950 AT5G13950 AT5G13950
fragaria_vesca	FvH4_6g54340 FvH4_6g54340 FvH4_6g54340 FvH4_6g54340
malus_domestica	MD09G1002200.v1.1 MD17G1006400.v1.1
prunus_persica	Prupe.3G314000_v2.0.a1 Prupe.3G314000_v2.0.a1 Prupe.3G314000_v2.0.a1
pyrus_communis	pycom111g00170 pycom17g00200
rosa_chinensis	RchiOBHm_Chr2g0176561
rosa_laevigata	RLG00000022425
rosa_roxburghii	Rroxscaffold_2G00076300
rosa_rugosa	Rorug02G0596200
rosa_samantha	Rh2AG674100 Rh2BG685300 Rh2CG648300 Rh2DG699200
rosa_wichuraiana	Rw2G055290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	544
Acc16I	TGCGCA	1 cut(s)	607
Acc36I	ACCTGC	2 cut(s)	544, 2447
AccII	CGCG	1 cut(s)	2539
AccIII	TCCGGA	1 cut(s)	1932
AciI	CCGC	9 cut(s)	5, 16, 225, 893, 1462, 1571, 1598, 2485, 2537
AclI	AACGTT	1 cut(s)	2064
AclWI	GGATC	8 cut(s)	186, 614, 627, 817, 830, 897, 2441, 2555
AcsI	RAATTY	4 cut(s)	292, 859, 2092, 2530
AcuI	CTGAAG	3 cut(s)	86, 1034, 1810
AcyI	GRCGYC	1 cut(s)	2249
AdeI	CACNNNGTG	1 cut(s)	1385
AfaI	GTAC	8 cut(s)	995, 1086, 1368, 1495, 1560, 1585, 2260, 2448
AfiI	CCNNNNNNNGG	4 cut(s)	1264, 2430, 2435, 2456
AflIII	ACRYGT	1 cut(s)	963
AgsI	TTSAA	9 cut(s)	114, 232, 595, 1190, 1292, 1304, 1736, 1865, 1979
AhlI	ACTAGT	1 cut(s)	2261
AjnI	CCWGG	3 cut(s)	59, 1040, 1628
AluBI	AGCT	9 cut(s)	612, 837, 1077, 1172, 1316, 1438, 2310, 2403, 2495
AluI	AGCT	9 cut(s)	612, 837, 1077, 1172, 1316, 1438, 2310, 2403, 2495
Alw21I	GWGCWC	1 cut(s)	1606
Alw26I	GTCTC	3 cut(s)	909, 1050, 1773
AlwI	GGATC	8 cut(s)	186, 614, 627, 817, 830, 897, 2441, 2555
AlwNI	CAGNNNCTG	2 cut(s)	1595, 1999
Ama87I	CYCGRG	1 cut(s)	1428
Aor13HI	TCCGGA	1 cut(s)	1932
AoxI	GGCC	6 cut(s)	209, 1879, 2014, 2099, 2112, 2421
ApeKI	GCWGC	3 cut(s)	1169, 1984, 2492
ApoI	RAATTY	4 cut(s)	292, 859, 2092, 2530
ArsI	GACNNNNNNTTYG	2 cut(s)	1207, 1239
Asp700I	GAANNNNTTC	3 cut(s)	536, 836, 1860
AspLEI	GCGC	2 cut(s)	608, 933
AspS9I	GGNCC	3 cut(s)	1879, 2113, 2421
AsuC2I	CCSGG	1 cut(s)	1166
AsuHPI	GGTGA	5 cut(s)	347, 488, 1289, 1397, 2231
AvaI	CYCGRG	1 cut(s)	1428
AxyI	CCTNAGG	1 cut(s)	270
BaeGI	GKGCMC	1 cut(s)	886
BaeI	ACNNNNGTAYC	2 cut(s)	2250, 2283
BamHI	GGATCC	2 cut(s)	619, 822
Bbv12I	GWGCWC	1 cut(s)	1606
BbvI	GCAGC	3 cut(s)	1181, 1996, 2504
BccI	CCATC	7 cut(s)	140, 492, 872, 944, 2069, 2177, 2235
BceAI	ACGGC	3 cut(s)	196, 1223, 1650
BciT130I	CCWGG	3 cut(s)	61, 1042, 1630
BclI	TGATCA	2 cut(s)	10, 1279
BcnI	CCSGG	1 cut(s)	1166
BcoDI	GTCTC	3 cut(s)	909, 1050, 1773
BcuI	ACTAGT	1 cut(s)	2261
BfaI	CTAG	4 cut(s)	375, 1617, 2262, 2397
BfmI	CTRYAG	3 cut(s)	940, 1587, 1773
BfuAI	ACCTGC	2 cut(s)	544, 2447
BglI	GCCNNNNNGGC	1 cut(s)	1634
BisI	GCNGC	4 cut(s)	6, 1170, 1985, 2493
BlsI	GCNGC	4 cut(s)	7, 1171, 1986, 2494
BmcAI	AGTACT	1 cut(s)	1585
Bme1390I	CCNGG	4 cut(s)	61, 1042, 1166, 1630
BmeT110I	CYCGRG	1 cut(s)	1428
BmgT120I	GGNCC	3 cut(s)	1879, 2113, 2421
BmiI	GGNNCC	6 cut(s)	621, 824, 1822, 2115, 2422, 2594
BmrFI	CCNGG	4 cut(s)	61, 1042, 1166, 1630
BmrI	ACTGGG	2 cut(s)	1365, 2111
BmsI	GCATC	7 cut(s)	481, 593, 1138, 1342, 1615, 2233, 2496
BmuI	ACTGGG	2 cut(s)	1365, 2111
BpmI	CTGGAG	1 cut(s)	2027
Bpu10I	CCTNAGC	1 cut(s)	472
BpuEI	CTTGAG	2 cut(s)	158, 737
BpuMI	CCSGG	1 cut(s)	1166
BsaAI	YACGTR	2 cut(s)	2368, 2558
BsaBI	GATNNNNATC	2 cut(s)	150, 483
BsaHI	GRCGYC	1 cut(s)	2249
BsaI	GGTCTC	1 cut(s)	1050
BsaJI	CCNNGG	3 cut(s)	60, 1344, 2429
BsaWI	WCCGGW	2 cut(s)	1053, 1932
BsaXI	ACNNNNNCTCC	2 cut(s)	2357, 2387
Bsc4I	CCNNNNNNNGG	4 cut(s)	1264, 2430, 2435, 2456
Bse118I	RCCGGY	1 cut(s)	47
Bse1I	ACTGG	8 cut(s)	1142, 1187, 1371, 1447, 1695, 1967, 1993, 2117
Bse21I	CCTNAGG	1 cut(s)	270
Bse3DI	GCAATG	4 cut(s)	42, 407, 2280, 2528
Bse8I	GATNNNNATC	2 cut(s)	150, 483
BseAI	TCCGGA	1 cut(s)	1932
BseBI	CCWGG	3 cut(s)	61, 1042, 1630
BseDI	CCNNGG	3 cut(s)	60, 1344, 2429
BseGI	GGATG	7 cut(s)	112, 151, 484, 1273, 2107, 2246, 2532
BseJI	GATNNNNATC	2 cut(s)	150, 483
BseLI	CCNNNNNNNGG	4 cut(s)	1264, 2430, 2435, 2456
BseMI	GCAATG	4 cut(s)	42, 407, 2280, 2528
BseMII	CTCAG	7 cut(s)	261, 330, 486, 804, 1571, 1896, 2049
BseNI	ACTGG	8 cut(s)	1142, 1187, 1371, 1447, 1695, 1967, 1993, 2117
BseRI	GAGGAG	2 cut(s)	819, 1539
BseSI	GKGCMC	1 cut(s)	886
BseXI	GCAGC	3 cut(s)	1181, 1996, 2504
BseYI	CCCAGC	1 cut(s)	416
BsgI	GTGCAG	2 cut(s)	659, 2147
Bsh1236I	CGCG	1 cut(s)	2539
BshFI	GGCC	6 cut(s)	211, 1881, 2016, 2101, 2114, 2423
BsiHKAI	GWGCWC	1 cut(s)	1606
BsiHKCI	CYCGRG	1 cut(s)	1428
BsiSI	CCGG	5 cut(s)	48, 1054, 1166, 1933, 2111
BslI	CCNNNNNNNGG	4 cut(s)	1264, 2430, 2435, 2456
BsmAI	GTCTC	3 cut(s)	909, 1050, 1773
BsmI	GAATGC	2 cut(s)	1032, 2302
BsnI	GGCC	6 cut(s)	211, 1881, 2016, 2101, 2114, 2423
Bso31I	GGTCTC	1 cut(s)	1050
BsoBI	CYCGRG	1 cut(s)	1428
Bsp1286I	GDGCHC	2 cut(s)	886, 1606
Bsp13I	TCCGGA	1 cut(s)	1932
Bsp19I	CCATGG	1 cut(s)	1344
BspACI	CCGC	9 cut(s)	5, 16, 225, 893, 1462, 1571, 1598, 2485, 2537
BspANI	GGCC	6 cut(s)	211, 1881, 2016, 2101, 2114, 2423
BspCNI	CTCAG	7 cut(s)	262, 331, 485, 803, 1572, 1897, 2050
BspEI	TCCGGA	1 cut(s)	1932
BspFNI	CGCG	1 cut(s)	2539
BspHI	TCATGA	2 cut(s)	2268, 2461
BspLI	GGNNCC	6 cut(s)	621, 824, 1822, 2115, 2422, 2594
BspMAI	CTGCAG	1 cut(s)	1591
BspMI	ACCTGC	2 cut(s)	544, 2447
BspPI	GGATC	8 cut(s)	186, 614, 627, 817, 830, 897, 2441, 2555
BspQI	GCTCTTC	1 cut(s)	1974
BspTNI	GGTCTC	1 cut(s)	1050
BsrDI	GCAATG	4 cut(s)	42, 407, 2280, 2528
BsrFI	RCCGGY	1 cut(s)	47
BsrI	ACTGG	8 cut(s)	1142, 1187, 1371, 1447, 1695, 1967, 1993, 2117
BssAI	RCCGGY	1 cut(s)	47
BssECI	CCNNGG	3 cut(s)	60, 1344, 2429
BssNI	GRCGYC	1 cut(s)	2249
BssT1I	CCWWGG	2 cut(s)	1344, 2429
Bst2UI	CCWGG	3 cut(s)	61, 1042, 1630
Bst4CI	ACNGT	2 cut(s)	1784, 2413
Bst6I	CTCTTC	3 cut(s)	593, 1974, 2015
BstACI	GRCGYC	1 cut(s)	2249
BstAPI	GCANNNNNTGC	7 cut(s)	409, 413, 890, 1141, 1175, 1595, 2230
BstBAI	YACGTR	2 cut(s)	2368, 2558
BstC8I	GCNNGC	8 cut(s)	405, 414, 610, 766, 1298, 1710, 1889, 2156
BstDEI	CTNAG	9 cut(s)	270, 339, 472, 790, 1580, 1747, 1905, 2058, 2179
BstDSI	CCRYGG	1 cut(s)	1344
BstEII	GGTNACC	1 cut(s)	1064
BstENI	CCTNNNNNAGG	1 cut(s)	1262
BstF5I	GGATG	7 cut(s)	112, 151, 484, 1273, 2107, 2246, 2532
BstFNI	CGCG	1 cut(s)	2539
BstHHI	GCGC	2 cut(s)	608, 933
BstMAI	GTCTC	3 cut(s)	909, 1050, 1773
BstNI	CCWGG	3 cut(s)	61, 1042, 1630
BstNSI	RCATGY	6 cut(s)	692, 768, 967, 1891, 2044, 2158
BstPI	GGTNACC	1 cut(s)	1064
BstSCI	CCNGG	4 cut(s)	59, 1040, 1164, 1628
BstSFI	CTRYAG	3 cut(s)	940, 1587, 1773
BstSLI	GKGCMC	1 cut(s)	886
BstUI	CGCG	1 cut(s)	2539
BstV1I	GCAGC	3 cut(s)	1181, 1996, 2504
BstX2I	RGATCY	3 cut(s)	619, 822, 902
BstXI	CCANNNNNNTGG	1 cut(s)	1408
BstYI	RGATCY	3 cut(s)	619, 822, 902
Bsu36I	CCTNAGG	1 cut(s)	270
BsuRI	GGCC	6 cut(s)	211, 1881, 2016, 2101, 2114, 2423
BtgI	CCRYGG	1 cut(s)	1344
BtgZI	GCGATG	1 cut(s)	963
BtsCI	GGATG	7 cut(s)	112, 151, 484, 1273, 2107, 2246, 2532
BtsI	GCAGTG	1 cut(s)	1997
BtsIMutI	CAGTG	7 cut(s)	798, 1135, 1702, 1789, 1974, 1997, 2323
BveI	ACCTGC	2 cut(s)	544, 2447
Cac8I	GCNNGC	8 cut(s)	405, 414, 610, 766, 1298, 1710, 1889, 2156
CaiI	CAGNNNCTG	2 cut(s)	1595, 1999
CciI	TCATGA	2 cut(s)	2268, 2461
CfoI	GCGC	2 cut(s)	608, 933
Cfr10I	RCCGGY	1 cut(s)	47
Cfr13I	GGNCC	3 cut(s)	1879, 2113, 2421
CseI	GACGC	2 cut(s)	2238, 2528
Csp6I	GTAC	8 cut(s)	994, 1085, 1367, 1494, 1559, 1584, 2259, 2447
CviQI	GTAC	8 cut(s)	994, 1085, 1367, 1494, 1559, 1584, 2259, 2447
DdeI	CTNAG	9 cut(s)	270, 339, 472, 790, 1580, 1747, 1905, 2058, 2179
DraIII	CACNNNGTG	1 cut(s)	1385
Eam1104I	CTCTTC	3 cut(s)	593, 1974, 2015
EarI	CTCTTC	3 cut(s)	593, 1974, 2015
Eco130I	CCWWGG	2 cut(s)	1344, 2429
Eco31I	GGTCTC	1 cut(s)	1050
Eco32I	GATATC	1 cut(s)	1162
Eco57I	CTGAAG	3 cut(s)	86, 1034, 1810
Eco81I	CCTNAGG	1 cut(s)	270
Eco88I	CYCGRG	1 cut(s)	1428
Eco91I	GGTNACC	1 cut(s)	1064
EcoNI	CCTNNNNNAGG	1 cut(s)	1262
EcoO65I	GGTNACC	1 cut(s)	1064
EcoRII	CCWGG	3 cut(s)	59, 1040, 1628
EcoRV	GATATC	1 cut(s)	1162
EcoT14I	CCWWGG	2 cut(s)	1344, 2429
EcoT22I	ATGCAT	2 cut(s)	1197, 1684
ErhI	CCWWGG	2 cut(s)	1344, 2429
FalI	AAGNNNNNCTT	4 cut(s)	291, 323, 396, 428
FauI	CCCGC	3 cut(s)	886, 1591, 2478
FauNDI	CATATG	3 cut(s)	313, 970, 1093
FbaI	TGATCA	2 cut(s)	10, 1279
Fnu4HI	GCNGC	4 cut(s)	6, 1170, 1985, 2493
FokI	GGATG	7 cut(s)	119, 158, 471, 1280, 2094, 2253, 2539
Fsp4HI	GCNGC	4 cut(s)	6, 1170, 1985, 2493
FspBI	CTAG	4 cut(s)	375, 1617, 2262, 2397
FspI	TGCGCA	1 cut(s)	607
GlaI	GCGC	2 cut(s)	607, 932
GluI	GCNGC	4 cut(s)	6, 1170, 1985, 2493
GsaI	CCCAGC	1 cut(s)	420
GsuI	CTGGAG	1 cut(s)	2027
HaeIII	GGCC	6 cut(s)	211, 1881, 2016, 2101, 2114, 2423
HapII	CCGG	5 cut(s)	48, 1054, 1166, 1933, 2111
HgaI	GACGC	2 cut(s)	2238, 2528
HhaI	GCGC	2 cut(s)	608, 933
Hin1I	GRCGYC	1 cut(s)	2249
Hin6I	GCGC	2 cut(s)	606, 931
HinP1I	GCGC	2 cut(s)	606, 931
HindIII	AAGCTT	3 cut(s)	835, 1436, 2308
HpaII	CCGG	5 cut(s)	48, 1054, 1166, 1933, 2111
HphI	GGTGA	5 cut(s)	347, 488, 1289, 1397, 2231
Hpy166II	GTNNAC	4 cut(s)	796, 2038, 2068, 2555
Hpy8I	GTNNAC	4 cut(s)	796, 2038, 2068, 2555
HpyAV	CCTTC	2 cut(s)	1225, 1834
HpyCH4III	ACNGT	2 cut(s)	1784, 2413
HpyCH4IV	ACGT	3 cut(s)	2064, 2367, 2557
HpyF3I	CTNAG	9 cut(s)	270, 339, 472, 790, 1580, 1747, 1905, 2058, 2179
HpySE526I	ACGT	3 cut(s)	2064, 2367, 2557
Hsp92I	GRCGYC	1 cut(s)	2249
HspAI	GCGC	2 cut(s)	606, 931
Kpn2I	TCCGGA	1 cut(s)	1932
Ksp22I	TGATCA	2 cut(s)	10, 1279
LguI	GCTCTTC	1 cut(s)	1974
LmnI	GCTCC	5 cut(s)	64, 1322, 1601, 2008, 2400
Lsp1109I	GCAGC	3 cut(s)	1181, 1996, 2504
LweI	GCATC	7 cut(s)	481, 593, 1138, 1342, 1615, 2233, 2496
MaeI	CTAG	4 cut(s)	375, 1617, 2262, 2397
MaeII	ACGT	3 cut(s)	2064, 2367, 2557
MaeIII	GTNAC	5 cut(s)	733, 868, 1064, 1995, 2333
MfeI	CAATTG	2 cut(s)	1145, 2339
MflI	RGATCY	3 cut(s)	619, 822, 902
MhlI	GDGCHC	2 cut(s)	886, 1606
MlyI	GAGTC	4 cut(s)	1508, 1769, 2209, 2281
MmeI	TCCRAC	1 cut(s)	1905
Mph1103I	ATGCAT	2 cut(s)	1197, 1684
MroI	TCCGGA	1 cut(s)	1932
MroXI	GAANNNNTTC	3 cut(s)	536, 836, 1860
MseI	TTAA	9 cut(s)	450, 656, 975, 1005, 1155, 1641, 1725, 1875, 2090
MslI	CAYNNNNRTG	5 cut(s)	489, 693, 1406, 1609, 2181
MspA1I	CMGCKG	1 cut(s)	16
MspI	CCGG	5 cut(s)	48, 1054, 1166, 1933, 2111
MspR9I	CCNGG	4 cut(s)	61, 1042, 1166, 1630
MunI	CAATTG	2 cut(s)	1145, 2339
Mva1269I	GAATGC	2 cut(s)	1032, 2302
MvaI	CCWGG	3 cut(s)	61, 1042, 1630
MvnI	CGCG	1 cut(s)	2539
NciI	CCSGG	1 cut(s)	1166
NcoI	CCATGG	1 cut(s)	1344
NdeI	CATATG	3 cut(s)	313, 970, 1093
NlaIV	GGNNCC	6 cut(s)	621, 824, 1822, 2115, 2422, 2594
NmuCI	GTSAC	1 cut(s)	1995
NsbI	TGCGCA	1 cut(s)	607
NsiI	ATGCAT	2 cut(s)	1197, 1684
NspI	RCATGY	6 cut(s)	692, 768, 967, 1891, 2044, 2158
PaeI	GCATGC	3 cut(s)	768, 1891, 2158
PaeR7I	CTCGAG	1 cut(s)	1428
PagI	TCATGA	2 cut(s)	2268, 2461
PaqCI	CACCTGC	1 cut(s)	544
PciI	ACATGT	1 cut(s)	963
PciSI	GCTCTTC	1 cut(s)	1974
PcsI	WCGNNNNNNNCGW	1 cut(s)	2594
PctI	GAATGC	2 cut(s)	1032, 2302
PdmI	GAANNNNTTC	3 cut(s)	536, 836, 1860
PkrI	GCNGC	4 cut(s)	7, 1171, 1986, 2494
PleI	GAGTC	4 cut(s)	1507, 1768, 2208, 2280
PpsI	GAGTC	4 cut(s)	1507, 1768, 2208, 2280
Ppu21I	YACGTR	2 cut(s)	2368, 2558
PscI	ACATGT	1 cut(s)	963
Psp1406I	AACGTT	1 cut(s)	2064
Psp6I	CCWGG	3 cut(s)	59, 1040, 1628
PspEI	GGTNACC	1 cut(s)	1064
PspFI	CCCAGC	1 cut(s)	416
PspGI	CCWGG	3 cut(s)	59, 1040, 1628
PspN4I	GGNNCC	6 cut(s)	621, 824, 1822, 2115, 2422, 2594
PspPI	GGNCC	3 cut(s)	1879, 2113, 2421
PstI	CTGCAG	1 cut(s)	1591
PstNI	CAGNNNCTG	2 cut(s)	1595, 1999
PsuI	RGATCY	3 cut(s)	619, 822, 902
RsaI	GTAC	8 cut(s)	995, 1086, 1368, 1495, 1560, 1585, 2260, 2448
RsaNI	GTAC	8 cut(s)	994, 1085, 1367, 1494, 1559, 1584, 2259, 2447
RseI	CAYNNNNRTG	5 cut(s)	489, 693, 1406, 1609, 2181
SapI	GCTCTTC	1 cut(s)	1974
SaqAI	TTAA	9 cut(s)	450, 656, 975, 1005, 1155, 1641, 1725, 1875, 2090
SatI	GCNGC	4 cut(s)	6, 1170, 1985, 2493
Sau96I	GGNCC	3 cut(s)	1879, 2113, 2421
ScaI	AGTACT	1 cut(s)	1585
SchI	GAGTC	4 cut(s)	1508, 1769, 2209, 2281
ScrFI	CCNGG	4 cut(s)	61, 1042, 1166, 1630
SduI	GDGCHC	2 cut(s)	886, 1606
SfaNI	GCATC	7 cut(s)	481, 593, 1138, 1342, 1615, 2233, 2496
SfcI	CTRYAG	3 cut(s)	940, 1587, 1773
Sfr274I	CTCGAG	1 cut(s)	1428
SlaI	CTCGAG	1 cut(s)	1428
SmiMI	CAYNNNNRTG	5 cut(s)	489, 693, 1406, 1609, 2181
SmlI	CTYRAG	4 cut(s)	137, 716, 1428, 2581
SmoI	CTYRAG	4 cut(s)	137, 716, 1428, 2581
SpeI	ACTAGT	1 cut(s)	2261
SphI	GCATGC	3 cut(s)	768, 1891, 2158
SsiI	CCGC	9 cut(s)	5, 16, 225, 893, 1462, 1571, 1598, 2485, 2537
SspMI	CTAG	4 cut(s)	375, 1617, 2262, 2397
StyD4I	CCNGG	4 cut(s)	59, 1040, 1164, 1628
StyI	CCWWGG	2 cut(s)	1344, 2429
TaaI	ACNGT	2 cut(s)	1784, 2413
TaiI	ACGT	3 cut(s)	2067, 2370, 2560
TaqI	TCGA	4 cut(s)	1429, 1849, 2096, 2121
TatI	WGTACW	2 cut(s)	1493, 1583
TauI	GCSGC	1 cut(s)	8
Tru1I	TTAA	9 cut(s)	450, 656, 975, 1005, 1155, 1641, 1725, 1875, 2090
Tru9I	TTAA	9 cut(s)	450, 656, 975, 1005, 1155, 1641, 1725, 1875, 2090
TscAI	CASTG	7 cut(s)	798, 1142, 1702, 1789, 1974, 2004, 2323
TseFI	GTSAC	1 cut(s)	1995
TseI	GCWGC	3 cut(s)	1169, 1984, 2492
Tsp45I	GTSAC	1 cut(s)	1995
TspGWI	ACGGA	1 cut(s)	815
TspRI	CASTG	7 cut(s)	798, 1142, 1702, 1789, 1974, 2004, 2323
XagI	CCTNNNNNAGG	1 cut(s)	1262
XapI	RAATTY	4 cut(s)	292, 859, 2092, 2530
XceI	RCATGY	6 cut(s)	692, 768, 967, 1891, 2044, 2158
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1951
XhoI	CTCGAG	1 cut(s)	1428
XmnI	GAANNNNTTC	3 cut(s)	536, 836, 1860
XspI	CTAG	4 cut(s)	375, 1617, 2262, 2397
ZrmI	AGTACT	1 cut(s)	1585
Zsp2I	ATGCAT	2 cut(s)	1197, 1684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.