Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0013293

Physical Location & Seq

Genomic Coordinates Forward (+)

503374 .. 505796

Gene Structure 2423 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1007800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1962 bp

ATGAAGATTTCAAAATCGAGTTTTTGTCGCATCCGCAGGCACCAATATTCGGTCGCAGTTAACAACCTCAACCTTGCAACCTGGTTCAACAAATACGTCGACAAAGACGATGTCGCTTCCTGGAACTCCGTCATTGCCGACTTGGCCCGGAGCGGCGACTCCCTCGAAGCCCTCCGGGCATTTTCGTCCATGCGAAAGCTTTCCCTCAGACCAAACCGCTCCTCCTTCCCCTGCGCCATCAAATCCTGCTCGGCTTTGCTGGACCTTCACTCCGGCAAGCAAGCTCACCAGCAGGCGTTGGTCTTCGGCTTTGAGTCCGATCTCTTTGTCTCCTCTGCACTCATCGATATGTACTCCAGATGTGGTCGATTGACGGATGCATGGAAACTGTTCGATACAATTCCTCACAGGAACGTGGTTACTTGGACGTCAATGATCACCGGGTGCGTCCAGAACGACGATGCCTGCCAAGCCTTGGCTCTCTTTAAAGAGTTGTTGATTGAGGAAATTGACAATGAGGATGTTTCTTTAGATCCTGTTGTACTGGTTTCTGTTCTGTCGGCTTGTTCTCGCGTGTCAAGTAAGGGACTTGCTCAAAGTGTTCATGGGTTGGTGGTGAAGAGAGGCTTTGATGGGGATTTATGGGTTGGGAATACTTTGATGGATGCATATGCCAAATGTGGCGAGCTCGGTCTTGTTAGAAAGGTGTTTGATGGAATGTCTCAAAAGGATTTGGTTTCTTGGAACTCCATGATTGCAACTTATGCACAGAGTGGATTATCTTCTGAGGCATGGCAAGTCTTCTACGGGATGGCAAGGGATGGTGACGTCCACTACAATGCGGTGACGTTGTCCACTTTGTTATTAGCTTGTGCACATGCTGGGCCTCTGCTAGTTGGGAAGTCTATACATGATCAGGTTCTGAAAAAGGGCTTGGAAGAGAATGTGATAGTGTCTACCTCTATTATCGACATGTACTGCAAACGTGGGAGAGTTGACTTGGCAAGGAAGGCATTTGATTGCATGAAGGAGAAGAATGTCAGGACATGGACTGCCATGGTTGCAGGTTATGGAATGCATGGCCATGCCAAAGAAGCGTTGGAGGTCTTCTATAAGATGATAAGAGATGGTGTGACACCAAATTACATAACATTTGTGTCACTTCTAAATGCTTGCAGCCATGCTGGTCTATTAGAACAGGGTTGGTATTGGTTTAATGCCATGAGCCGCAAATTCGGTGTAGAACCTGGAATTGAGCACTACGGGTGCATGGTTGATCTTCTTGGACGTTCTGGTGATCTTAACAAGGCTTATGATTTAATAAAGGGAATGAGGATCATGCCAGATTGTGTTGTCTGGGGTTCGTTTCTTGGGGCTTGCAGGGTACACAAAAATGTGACATTTGCAGAGATTTCTGCAAGAAAACTATTTGAGCTTGACCCTCATAATTGTGGGTACTACATCTTGCTTTCAAATATATATGCCGATGCTGGGAGATGGGAAGATGTTGAGAGGATGAGAGTACTCATGAAGAATCGTGGGTTGGTAAAACCTCCTGGATTCAGTTTGGTTGAACTGAGAGGTATTGTGCATTTATTTTTAGTTGGAGACAGAGAGCATCCTCAGCATGAGAAGATTTACGAGTATTTGGAAAAACTGACAATCAAGCTGCAAGAGGTTGGCTATGCACCCAATATCAGTTCAGTTCTTCATGACGTTGATGAGGAAGAGAAGGAAATGGTCCTAAGAGTACATAGTGAGAAGCTGGCCGTTGCCTTTGGAATCATGAATTCTGTATCTGGTGCGACGATTCAGATAATCAAGAATCTTCGAGTTTGTGCCAATTGTCATACTGTTATTAAGTTGATTACGAAGGTTGTTGATCGGGAAATTGTGGTCAGAGATTCAAAGCGGTTTCATCACTTCAGGAATGGGTTGTGTTCTTGTGGAGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

654

Amino Acids

73.25

Weight (kDa)

8.14

Isoelectric Point (pI)

38.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	244 - 293	5.2e-06	PPR repeat family
PPR_2	PF13041	345 - 393	5.7e-10	PPR repeat family
PPR_3	PF13812	346 - 392	1.3e-06	Pentatricopeptide repeat domain
PPR	PF01535	349 - 378	2.2e-07	PPR repeat
E_motif	PF20431	463 - 525	5.1e-21	E motif
DYW_deaminase	PF14432	561 - 653	2.2e-42	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013293)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26782
fragaria_vesca	FvH4_6g54200 FvH4_6g54200 FvH4_6g54200
malus_domestica	MD09G1004300.v1.1 MD17G1007800.v1.1
prunus_persica	Prupe.3G312400_v2.0.a1
pyrus_communis	pycom111g00310 pycom17g00330
rosa_chinensis	RchiOBHm_Chr2g0176381
rosa_laevigata	RLG00000022408
rosa_multiflora	Rmu_co8497233.1_g000001 Rmu_sc0009140.1_g000003
rosa_roxburghii	Rroxscaffold_2G00076480
rosa_rugosa	Rorug02G0593800
rosa_samantha	Rh2BG683700 Rh2CG646400
rosa_wichuraiana	Rw2G055130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	431, 831
Acc36I	ACCTGC	1 cut(s)	1055
AccB1I	GGYRCC	1 cut(s)	39
AccB7I	CCANNNNNTGG	1 cut(s)	475
AccBSI	CCGCTC	2 cut(s)	153, 219
AccI	GTMKAC	2 cut(s)	99, 956
AccII	CGCG	1 cut(s)	573
AciI	CCGC	6 cut(s)	34, 153, 217, 842, 1228, 1912
AclWI	GGATC	2 cut(s)	527, 1343
AcoI	YGGCCR	2 cut(s)	1081, 1767
AcsI	RAATTY	2 cut(s)	1232, 1789
AcuI	CTGAAG	1 cut(s)	1909
AcyI	GRCGYC	2 cut(s)	428, 828
AdeI	CACNNNGTG	2 cut(s)	444, 773
AfaI	GTAC	7 cut(s)	353, 543, 977, 1386, 1457, 1524, 1752
AfiI	CCNNNNNNNGG	3 cut(s)	49, 475, 1491
AflIII	ACRYGT	1 cut(s)	972
AgsI	TTSAA	5 cut(s)	12, 88, 1473, 1574, 1908
AjnI	CCWGG	4 cut(s)	80, 119, 1246, 1555
AjuI	GAANNNNNNNTTGG	4 cut(s)	917, 949, 1526, 1558
AluBI	AGCT	7 cut(s)	199, 284, 688, 869, 1435, 1669, 1765
AluI	AGCT	7 cut(s)	199, 284, 688, 869, 1435, 1669, 1765
Alw21I	GWGCWC	3 cut(s)	690, 877, 1260
Alw26I	GTCTC	3 cut(s)	334, 726, 1602
Alw44I	GTGCAC	1 cut(s)	873
AlwI	GGATC	2 cut(s)	527, 1343
AlwNI	CAGNNNCTG	1 cut(s)	922
AoxI	GGCC	4 cut(s)	144, 884, 1081, 1767
ApaLI	GTGCAC	1 cut(s)	873
ApeKI	GCWGC	2 cut(s)	1176, 1669
ApoI	RAATTY	2 cut(s)	1232, 1789
ArsI	GACNNNNNNTTYG	2 cut(s)	96, 128
Asp700I	GAANNNNTTC	2 cut(s)	199, 389
AspLEI	GCGC	1 cut(s)	236
AspS9I	GGNCC	4 cut(s)	145, 262, 884, 1741
AsuC2I	CCSGG	3 cut(s)	148, 176, 442
AsuHPI	GGTGA	6 cut(s)	278, 430, 628, 836, 856, 1307
AvaII	GGWCC	2 cut(s)	262, 1741
BaeGI	GKGCMC	1 cut(s)	877
BalI	TGGCCA	1 cut(s)	1083
BanI	GGYRCC	1 cut(s)	39
BanII	GRGCYC	1 cut(s)	690
BbsI	GAAGAC	3 cut(s)	295, 793, 1099
Bbv12I	GWGCWC	3 cut(s)	690, 877, 1260
BbvCI	CCTCAGC	1 cut(s)	1623
BbvI	GCAGC	2 cut(s)	1188, 1656
BccI	CCATC	8 cut(s)	245, 626, 655, 707, 805, 815, 1121, 1491
BceAI	ACGGC	1 cut(s)	1754
BciT130I	CCWGG	4 cut(s)	82, 121, 1248, 1557
BclI	TGATCA	2 cut(s)	435, 913
BcnI	CCSGG	3 cut(s)	148, 176, 442
BcoDI	GTCTC	3 cut(s)	334, 726, 1602
BfaI	CTAG	1 cut(s)	893
BfuAI	ACCTGC	1 cut(s)	1055
BglI	GCCNNNNNGGC	2 cut(s)	143, 176
BisI	GCNGC	4 cut(s)	154, 1177, 1228, 1670
BlsI	GCNGC	4 cut(s)	155, 1178, 1229, 1671
BmcAI	AGTACT	1 cut(s)	1524
Bme1390I	CCNGG	7 cut(s)	82, 121, 148, 176, 442, 1248, 1557
Bme18I	GGWCC	2 cut(s)	262, 1741
BmgT120I	GGNCC	4 cut(s)	145, 262, 884, 1741
BmiI	GGNNCC	1 cut(s)	41
BmrFI	CCNGG	7 cut(s)	82, 121, 148, 176, 442, 1248, 1557
BmsI	GCATC	6 cut(s)	39, 367, 451, 655, 1477, 1627
BpiI	GAAGAC	3 cut(s)	295, 793, 1099
BpmI	CTGGAG	1 cut(s)	340
Bpu10I	CCTNAGC	1 cut(s)	1623
BpuMI	CCSGG	3 cut(s)	148, 176, 442
Bsa29I	ATCGAT	1 cut(s)	345
BsaHI	GRCGYC	2 cut(s)	428, 828
BsaJI	CCNNGG	2 cut(s)	474, 1056
BsaXI	ACNNNNNCTCC	4 cut(s)	206, 236, 254, 284
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 475, 1491
Bse1I	ACTGG	1 cut(s)	549
Bse3DI	GCAATG	1 cut(s)	132
BseBI	CCWGG	4 cut(s)	82, 121, 1248, 1557
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	2 cut(s)	474, 1056
BseGI	GGATG	8 cut(s)	30, 382, 526, 670, 816, 826, 1521, 1618
BseLI	CCNNNNNNNGG	3 cut(s)	49, 475, 1491
BseMI	GCAATG	1 cut(s)	132
BseMII	CTCAG	4 cut(s)	220, 777, 1568, 1637
BseNI	ACTGG	1 cut(s)	549
BseRI	GAGGAG	2 cut(s)	211, 322
BseSI	GKGCMC	1 cut(s)	877
BseXI	GCAGC	2 cut(s)	1188, 1656
BseYI	CCCAGC	2 cut(s)	881, 1490
BsgI	GTGCAG	1 cut(s)	321
Bsh1236I	CGCG	1 cut(s)	573
Bsh1285I	CGRYCG	1 cut(s)	54
BshFI	GGCC	4 cut(s)	146, 886, 1083, 1769
BshNI	GGYRCC	1 cut(s)	39
BshVI	ATCGAT	1 cut(s)	345
BsiEI	CGRYCG	1 cut(s)	54
BsiHKAI	GWGCWC	3 cut(s)	690, 877, 1260
BsiSI	CCGG	4 cut(s)	148, 175, 273, 441
BslFI	GGGAC	1 cut(s)	600
BslI	CCNNNNNNNGG	3 cut(s)	49, 475, 1491
BsmAI	GTCTC	3 cut(s)	334, 726, 1602
BsmFI	GGGAC	1 cut(s)	600
BsmI	GAATGC	1 cut(s)	1080
BsnI	GGCC	4 cut(s)	146, 886, 1083, 1769
Bsp1286I	GDGCHC	3 cut(s)	690, 877, 1260
Bsp143I	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
Bsp19I	CCATGG	1 cut(s)	1056
BspACI	CCGC	6 cut(s)	34, 153, 217, 842, 1228, 1912
BspANI	GGCC	4 cut(s)	146, 886, 1083, 1769
BspCNI	CTCAG	4 cut(s)	219, 778, 1569, 1636
BspDI	ATCGAT	1 cut(s)	345
BspFNI	CGCG	1 cut(s)	573
BspHI	TCATGA	3 cut(s)	1527, 1711, 1785
BspLI	GGNNCC	1 cut(s)	41
BspMI	ACCTGC	1 cut(s)	1055
BspPI	GGATC	2 cut(s)	527, 1343
BspT107I	GGYRCC	1 cut(s)	39
BsrBI	CCGCTC	2 cut(s)	153, 219
BsrDI	GCAATG	1 cut(s)	132
BsrI	ACTGG	1 cut(s)	549
BssECI	CCNNGG	2 cut(s)	474, 1056
BssMI	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
BssNI	GRCGYC	2 cut(s)	428, 828
BssT1I	CCWWGG	2 cut(s)	474, 1056
Bst2UI	CCWGG	4 cut(s)	82, 121, 1248, 1557
Bst4CI	ACNGT	2 cut(s)	390, 1855
Bst6I	CTCTTC	3 cut(s)	614, 933, 1722
BstACI	GRCGYC	2 cut(s)	428, 828
BstAPI	GCANNNNNTGC	1 cut(s)	764
BstC8I	GCNNGC	9 cut(s)	38, 278, 282, 294, 466, 686, 1174, 1378, 1767
BstDEI	CTNAG	5 cut(s)	206, 786, 1577, 1623, 1745
BstDSI	CCRYGG	1 cut(s)	1056
BstF5I	GGATG	8 cut(s)	30, 382, 526, 670, 816, 826, 1521, 1618
BstFNI	CGCG	1 cut(s)	573
BstHHI	GCGC	1 cut(s)	236
BstKTI	GATC	8 cut(s)	322, 438, 535, 916, 1279, 1300, 1338, 1885
BstMAI	GTCTC	3 cut(s)	334, 726, 1602
BstMBI	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
BstMCI	CGRYCG	1 cut(s)	54
BstMWI	GCNNNNNNNGC	8 cut(s)	143, 176, 470, 764, 1010, 1061, 1094, 1624
BstNI	CCWGG	4 cut(s)	82, 121, 1248, 1557
BstNSI	RCATGY	2 cut(s)	881, 976
BstSCI	CCNGG	7 cut(s)	80, 119, 146, 174, 440, 1246, 1555
BstSLI	GKGCMC	1 cut(s)	877
BstUI	CGCG	1 cut(s)	573
BstV1I	GCAGC	2 cut(s)	1188, 1656
BstV2I	GAAGAC	3 cut(s)	295, 793, 1099
BstX2I	RGATCY	1 cut(s)	532
BstYI	RGATCY	1 cut(s)	532
Bsu15I	ATCGAT	1 cut(s)	345
BsuRI	GGCC	4 cut(s)	146, 886, 1083, 1769
BsuTUI	ATCGAT	1 cut(s)	345
BtgI	CCRYGG	1 cut(s)	1056
BtsCI	GGATG	8 cut(s)	30, 382, 526, 670, 816, 826, 1521, 1618
BveI	ACCTGC	1 cut(s)	1055
Cac8I	GCNNGC	9 cut(s)	38, 278, 282, 294, 466, 686, 1174, 1378, 1767
CaiI	CAGNNNCTG	1 cut(s)	922
CciI	TCATGA	3 cut(s)	1527, 1711, 1785
CfoI	GCGC	1 cut(s)	236
Cfr13I	GGNCC	4 cut(s)	145, 262, 884, 1741
ClaI	ATCGAT	1 cut(s)	345
CseI	GACGC	1 cut(s)	436
CsiI	ACCWGGT	1 cut(s)	80
Csp6I	GTAC	7 cut(s)	352, 542, 976, 1385, 1456, 1523, 1751
CviQI	GTAC	7 cut(s)	352, 542, 976, 1385, 1456, 1523, 1751
DdeI	CTNAG	5 cut(s)	206, 786, 1577, 1623, 1745
DpnI	GATC	8 cut(s)	321, 437, 534, 915, 1278, 1299, 1337, 1884
DpnII	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
DraI	TTTAAA	1 cut(s)	487
DraIII	CACNNNGTG	2 cut(s)	444, 773
EaeI	YGGCCR	2 cut(s)	1081, 1767
Eam1104I	CTCTTC	3 cut(s)	614, 933, 1722
EarI	CTCTTC	3 cut(s)	614, 933, 1722
Ecl136II	GAGCTC	1 cut(s)	688
Eco130I	CCWWGG	2 cut(s)	474, 1056
Eco24I	GRGCYC	1 cut(s)	690
Eco47I	GGWCC	2 cut(s)	262, 1741
Eco53kI	GAGCTC	1 cut(s)	688
Eco57I	CTGAAG	1 cut(s)	1909
EcoICRI	GAGCTC	1 cut(s)	688
EcoRI	GAATTC	1 cut(s)	1789
EcoRII	CCWGG	4 cut(s)	80, 119, 1246, 1555
EcoT14I	CCWWGG	2 cut(s)	474, 1056
EcoT22I	ATGCAT	3 cut(s)	382, 670, 1080
EcoT38I	GRGCYC	1 cut(s)	690
ErhI	CCWWGG	2 cut(s)	474, 1056
FalI	AAGNNNNNCTT	2 cut(s)	611, 643
FaqI	GGGAC	1 cut(s)	600
FauNDI	CATATG	1 cut(s)	670
FbaI	TGATCA	2 cut(s)	435, 913
FblI	GTMKAC	2 cut(s)	99, 956
Fnu4HI	GCNGC	4 cut(s)	154, 1177, 1228, 1670
FokI	GGATG	8 cut(s)	17, 389, 533, 677, 823, 833, 1528, 1605
FriOI	GRGCYC	1 cut(s)	690
Fsp4HI	GCNGC	4 cut(s)	154, 1177, 1228, 1670
FspBI	CTAG	1 cut(s)	893
GlaI	GCGC	1 cut(s)	235
GluI	GCNGC	4 cut(s)	154, 1177, 1228, 1670
GsaI	CCCAGC	2 cut(s)	885, 1494
GsuI	CTGGAG	1 cut(s)	340
HaeIII	GGCC	4 cut(s)	146, 886, 1083, 1769
HapII	CCGG	4 cut(s)	148, 175, 273, 441
HgaI	GACGC	1 cut(s)	436
HhaI	GCGC	1 cut(s)	236
Hin1I	GRCGYC	2 cut(s)	428, 828
Hin6I	GCGC	1 cut(s)	234
HinP1I	GCGC	1 cut(s)	234
HincII	GTYRAC	3 cut(s)	61, 100, 997
HindII	GTYRAC	3 cut(s)	61, 100, 997
HindIII	AAGCTT	1 cut(s)	197
HinfI	GANTC	8 cut(s)	158, 314, 1534, 1560, 1782, 1810, 1825, 1904
HpaI	GTTAAC	1 cut(s)	61
HpaII	CCGG	4 cut(s)	148, 175, 273, 441
HphI	GGTGA	6 cut(s)	278, 430, 628, 836, 856, 1307
Hpy166II	GTNNAC	8 cut(s)	61, 100, 832, 855, 875, 957, 997, 1387
Hpy188I	TCNGA	6 cut(s)	209, 319, 787, 924, 1815, 1901
Hpy188III	TCNNGA	9 cut(s)	357, 451, 1042, 1528, 1712, 1786, 1822, 1886, 1927
Hpy8I	GTNNAC	8 cut(s)	61, 100, 832, 855, 875, 957, 997, 1387
Hpy99I	CGWCG	3 cut(s)	101, 461, 1810
HpyAV	CCTTC	6 cut(s)	235, 275, 1003, 1021, 1726, 1867
HpyCH4III	ACNGT	2 cut(s)	390, 1855
HpyCH4IV	ACGT	8 cut(s)	96, 414, 428, 828, 848, 985, 1288, 1716
HpyF10VI	GCNNNNNNNGC	8 cut(s)	143, 176, 470, 764, 1010, 1061, 1094, 1624
HpyF3I	CTNAG	5 cut(s)	206, 786, 1577, 1623, 1745
HpySE526I	ACGT	8 cut(s)	96, 414, 428, 828, 848, 985, 1288, 1716
Hsp92I	GRCGYC	2 cut(s)	428, 828
HspAI	GCGC	1 cut(s)	234
Ksp22I	TGATCA	2 cut(s)	435, 913
KspAI	GTTAAC	1 cut(s)	61
Kzo9I	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
LmnI	GCTCC	2 cut(s)	150, 224
Lsp1109I	GCAGC	2 cut(s)	1188, 1656
LweI	GCATC	6 cut(s)	39, 367, 451, 655, 1477, 1627
MabI	ACCWGGT	1 cut(s)	80
MaeI	CTAG	1 cut(s)	893
MaeII	ACGT	8 cut(s)	96, 414, 428, 828, 848, 985, 1288, 1716
MaeIII	GTNAC	6 cut(s)	418, 824, 844, 1132, 1158, 1396
MalI	GATC	8 cut(s)	321, 437, 534, 915, 1278, 1299, 1337, 1884
MbiI	CCGCTC	2 cut(s)	153, 219
MboI	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
MfeI	CAATTG	1 cut(s)	1843
MflI	RGATCY	1 cut(s)	532
MhlI	GDGCHC	3 cut(s)	690, 877, 1260
MlsI	TGGCCA	1 cut(s)	1083
MluCI	AATT	9 cut(s)	399, 507, 1141, 1232, 1251, 1447, 1789, 1843, 1890
MluNI	TGGCCA	1 cut(s)	1083
MlyI	GAGTC	2 cut(s)	152, 323
MmeI	TCCRAC	2 cut(s)	1080, 1585
Mox20I	TGGCCA	1 cut(s)	1083
Mph1103I	ATGCAT	3 cut(s)	382, 670, 1080
MroXI	GAANNNNTTC	2 cut(s)	199, 389
MscI	TGGCCA	1 cut(s)	1083
MseI	TTAA	6 cut(s)	60, 486, 1215, 1302, 1319, 1860
MslI	CAYNNNNRTG	5 cut(s)	347, 837, 1083, 1392, 1449
Msp20I	TGGCCA	1 cut(s)	1083
MspI	CCGG	4 cut(s)	148, 175, 273, 441
MspR9I	CCNGG	7 cut(s)	82, 121, 148, 176, 442, 1248, 1557
MunI	CAATTG	1 cut(s)	1843
Mva1269I	GAATGC	1 cut(s)	1080
MvaI	CCWGG	4 cut(s)	82, 121, 1248, 1557
MvnI	CGCG	1 cut(s)	573
MwoI	GCNNNNNNNGC	8 cut(s)	143, 176, 470, 764, 1010, 1061, 1094, 1624
NciI	CCSGG	3 cut(s)	148, 176, 442
NcoI	CCATGG	1 cut(s)	1056
NdeI	CATATG	1 cut(s)	670
NdeII	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
NlaIV	GGNNCC	1 cut(s)	41
NmeAIII	GCCGAG	1 cut(s)	230
NmuCI	GTSAC	5 cut(s)	824, 844, 1132, 1158, 1396
NsiI	ATGCAT	3 cut(s)	382, 670, 1080
NspI	RCATGY	2 cut(s)	881, 976
PagI	TCATGA	3 cut(s)	1527, 1711, 1785
PciI	ACATGT	1 cut(s)	972
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	1 cut(s)	1080
PdmI	GAANNNNTTC	2 cut(s)	199, 389
PfeI	GAWTC	6 cut(s)	1534, 1560, 1782, 1810, 1825, 1904
PflFI	GACNNNGTC	2 cut(s)	110, 850
PflMI	CCANNNNNTGG	1 cut(s)	475
PfoI	TCCNGGA	2 cut(s)	119, 1555
PkrI	GCNGC	4 cut(s)	155, 1178, 1229, 1671
PleI	GAGTC	2 cut(s)	152, 322
PpsI	GAGTC	2 cut(s)	152, 322
PscI	ACATGT	1 cut(s)	972
Psp124BI	GAGCTC	1 cut(s)	690
Psp6I	CCWGG	4 cut(s)	80, 119, 1246, 1555
PspFI	CCCAGC	2 cut(s)	881, 1490
PspGI	CCWGG	4 cut(s)	80, 119, 1246, 1555
PspN4I	GGNNCC	1 cut(s)	41
PspPI	GGNCC	4 cut(s)	145, 262, 884, 1741
PstNI	CAGNNNCTG	1 cut(s)	922
PsuI	RGATCY	1 cut(s)	532
PsyI	GACNNNGTC	2 cut(s)	110, 850
RsaI	GTAC	7 cut(s)	353, 543, 977, 1386, 1457, 1524, 1752
RsaNI	GTAC	7 cut(s)	352, 542, 976, 1385, 1456, 1523, 1751
RseI	CAYNNNNRTG	5 cut(s)	347, 837, 1083, 1392, 1449
SacI	GAGCTC	1 cut(s)	690
SalI	GTCGAC	1 cut(s)	98
SaqAI	TTAA	6 cut(s)	60, 486, 1215, 1302, 1319, 1860
SatI	GCNGC	4 cut(s)	154, 1177, 1228, 1670
Sau3AI	GATC	8 cut(s)	319, 435, 532, 913, 1276, 1297, 1335, 1882
Sau96I	GGNCC	4 cut(s)	145, 262, 884, 1741
ScaI	AGTACT	1 cut(s)	1524
SchI	GAGTC	2 cut(s)	152, 323
ScrFI	CCNGG	7 cut(s)	82, 121, 148, 176, 442, 1248, 1557
SduI	GDGCHC	3 cut(s)	690, 877, 1260
SexAI	ACCWGGT	1 cut(s)	80
SfaNI	GCATC	6 cut(s)	39, 367, 451, 655, 1477, 1627
SinI	GGWCC	2 cut(s)	262, 1741
SmiMI	CAYNNNNRTG	5 cut(s)	347, 837, 1083, 1392, 1449
Sse9I	AATT	9 cut(s)	399, 507, 1141, 1232, 1251, 1447, 1789, 1843, 1890
SsiI	CCGC	6 cut(s)	34, 153, 217, 842, 1228, 1912
SspI	AATATT	1 cut(s)	47
SspMI	CTAG	1 cut(s)	893
SstI	GAGCTC	1 cut(s)	690
StyD4I	CCNGG	7 cut(s)	80, 119, 146, 174, 440, 1246, 1555
StyI	CCWWGG	2 cut(s)	474, 1056
TaaI	ACNGT	2 cut(s)	390, 1855
TaiI	ACGT	8 cut(s)	99, 417, 431, 831, 851, 988, 1291, 1719
TaqI	TCGA	8 cut(s)	17, 99, 165, 345, 367, 393, 969, 1831
TaqII	GACCGA	2 cut(s)	40, 680
TasI	AATT	9 cut(s)	399, 507, 1141, 1232, 1251, 1447, 1789, 1843, 1890
TatI	WGTACW	5 cut(s)	351, 541, 975, 1522, 1750
TauI	GCSGC	2 cut(s)	156, 1230
TfiI	GAWTC	6 cut(s)	1534, 1560, 1782, 1810, 1825, 1904
Tru1I	TTAA	6 cut(s)	60, 486, 1215, 1302, 1319, 1860
Tru9I	TTAA	6 cut(s)	60, 486, 1215, 1302, 1319, 1860
TseFI	GTSAC	5 cut(s)	824, 844, 1132, 1158, 1396
TseI	GCWGC	2 cut(s)	1176, 1669
Tsp45I	GTSAC	5 cut(s)	824, 844, 1132, 1158, 1396
TspDTI	ATGAA	7 cut(s)	17, 593, 1040, 1544, 1700, 1802, 1907
TspGWI	ACGGA	2 cut(s)	118, 389
Tth111I	GACNNNGTC	2 cut(s)	110, 850
Van91I	CCANNNNNTGG	1 cut(s)	475
VneI	GTGCAC	1 cut(s)	873
VpaK11BI	GGWCC	2 cut(s)	262, 1741
XapI	RAATTY	2 cut(s)	1232, 1789
XceI	RCATGY	2 cut(s)	881, 976
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1096
XmiI	GTMKAC	2 cut(s)	99, 956
XmnI	GAANNNNTTC	2 cut(s)	199, 389
XspI	CTAG	1 cut(s)	893
ZraI	GACGTC	2 cut(s)	429, 829
ZrmI	AGTACT	1 cut(s)	1524
Zsp2I	ATGCAT	3 cut(s)	382, 670, 1080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.