Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0015244

Physical Location & Seq

Genomic Coordinates Forward (+)

3089322 .. 3092964

Gene Structure 3643 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1042100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGGCCGATCACCACCACCACCACCACCACCAGCACAGGCCTCACCGTCTCTCACTCCCCCAGCGTCCCACCACCCCCACCACCTTCTCCACCACTACACCCACCGCCTTCTCCGCCACACCCACCACCGCCACATCTAGACAGTACCCTGTTTACCCTTTCTCATCGACCCCGATTGCAACTCCGTCCAAATACCGCCTCTCATCCCTCAACCCCAAACCGTTCAACCCCAACAGCAGCAAGTCCTCCCTCTCCTTCCTCTTCCTCCTCCTCCTCTCCCTCCGCTCCCTCTATTCCCTCCTCCCTTTCCTCCGCTCCTCCCCTTCTTTCTCTCTCTTCCCCTTCTCTTTCCTCGTCTCTCTCCTCTCCTTCCTCCTCACCCTCTCCTCTTCCCTCCTCACCTCCTCCTCCTCTTCCAAAGACCCTTTTCACCAAAAGCAAAACCAACCCATCTTCTCTCTCACCTTGATCACACAGTCCCAGCACAGAATCTTGGTTGCCAAATCGATTCTTCTTGCCGTGGTCTTCCTCCTCCGGTTTCAGGCGCTTCGTTATTGCGGCACCGCCGCCATGATCCTGGCAGAATTGTCCGGCAACGTGGCGGCCCGGTTCTTGGCGGATGGCCATGATTCGAGTGTCGGCGGCGATAGGAATCGGTCCTCCAAGGTCCGTGGGTTTCTTGCTCTGTTTTCTGGGTTATTTTTGTTATCTATGAGTTGGGATCGGATTGACTGCTTCCCATTTTCTGCTAAATTGGTTGAAAGTTTGGGACTTTCACTGTTTCCAAGAGTCAATTGCGTGAGAATTTGGCCAATGATGCTTCCATTTCTCGCTGGATTTTTGGGTTGCTACGAACGGGTATCGATGAATTGGGGAACAATTAGGCAATTGGGTCGAAAACGGGTTCGTGTAATTTCGCTGTTTTTTACAACTGTTGTGCTGTTCATTCCTGCTGTTGTGAGTTTTTTCATGTTTGAAGCTGTGGGAGATGGAGTTTCAGTGGGAACTTTAGTTTGGCCTCTGGCAAACACCGTTGTTTTTGGGGTGCTTCTGAGTGAAAGTTTTAGTGATGACAAGTCATTGAGTTCAAACGATTTCCGAAGAGAGTATATGGTAACATTTCTGTGTACTCTTGTGTTGGAGTTGTTCTATTTTCCTGAATTATCACTTTGGGGACTGTTGCTATGTGGGTTGTTACTGTATGTTGCTGTTAGGGAATTGGATCCTTACTATTTGAAATATCTTGAACTGGGGATGGAGTCTTCAGAATCGTTTACTACCGCCATTATGAAGCCGATTAAGCACATTTTGAGTGAGAGGAAGTCACGCAAGATTGCACTTTTCCTTATGATCAATACTGGATACATGTTTGTGGAATTTGCTGCTGGGTTCATGAGCAATAGTCTGGGGCTGATATCTGATGCCTGTCACATGTTGTTTGATTGTGCTGCTCTTGCAATTGGGCTATATGCTTCGTATATTTCTCGCTTGCCTGCAAACAATCAGTATAATTATGGGCGTGGGAGATTTGAGGTTCTTTCGGGATATACTAATGCTGTTTTCCTGGTGTTGGTTGGATTGTTGATAGTTTTGGAGTCGTTTGAGAGGATTTTGGACCCTCAGGAGATATCGACTAGCAGTTTATTAGTTGTTTCAATCGGGGGGCTTCTTGTTAATGTGGTTGGTTTAATCTTCTTTCACGAGGAGCATCATCATGCTCATGGTGGATCAGGATCATGCTCCCACTCCCACCATCACCCGCATCCGCATGACTCTGTTGGGCATAATCATGAAGGTCATGGAAAGCATGAGGAATGCATACCTGTTTTCAGTGAAGACCATGAAAAATCATGTTCTGGCCATCATGACCATCATCATGAACAACAACATGGCAGTTCTGTCAGTTCTAAAGATCACAATTGCGGAAGCAAAGAGTGCCATGATCACCAACATGATCAGCATGACCACCATGGCCATAGTAAGCATGACAGCCACGTTCATAGCCACCACCATGACCAACATGATCATGCCCACCAACATCATCACCATGACCATGCCCACCAGCATGAACATCATGACCATGCCCACCAGCACGAACACCATGACCATGCCCACCTGCACGAACACCATGACCATGCCCACCAGCACGAACACCATGACCATGCCCACCAGCACGAACACCATGACCACGCCCACACTCATGGAAGCTTACCCCATCAAGTGGCGGAATCTCACATTCAAGAAGCAGGTTTCCATAGCCATAAACAGCCAATTGATGGAGAAAAATCAGCTAAGCATCAACATCGCCACATTGACCACAACATGGAAGGAATATTTTTGCACGTTTTGGCTGACACCTTGGGAAGTGTCGGAGTTGTGATATCAACCCTCTTGATTAAGTACAAGGGATGGCTGGTTGCTGATCCTGCCTGCTCGATATTCATTTCCGTCTTGATTATATCTTCGGTTATCCCATTACTTAGAAACTCGGCAGAAATCTTGCTCCAAAGAATTCCCAGAGCACACGAGCAGGATTTGAGAAAAGCTCTTATCGAGGTTAGGAAGATAAGGGGGGTTTCTGGCATTCGGAACTGGCACATATGGAGTTTCACAAACAGCGACGTAGTGGGGACTCTCCATCTCCATGTCTCAACAGAAATCGACAAGGCTGCTGCAAAGGCAAAGGTCTCACACGTACTACACGAAGCTGGAGTCAAGGACTTAACATTGCAGTTGGAATGCGTTGGATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0071578 GO:0072509 GO:0072511 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098739 GO:0099587

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

918

Amino Acids

103.12

Weight (kDa)

6.87

Isoelectric Point (pI)

38.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	446 - 573	1.5e-33	Cation efflux transmembrane domain
Cation_efflux	PF01545	765 - 835	1.5e-13	Cation efflux transmembrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04620 AT2G04620
fragaria_vesca	FvH4_6g49950
malus_domestica	MD17G1042100.v1.1
prunus_persica	Prupe.3G277100_v2.0.a1
pyrus_communis	pycom17g03760
rosa_chinensis	RchiOBHm_Chr2g0170121
rosa_laevigata	RLG00000021925
rosa_multiflora	Rmu_sc0013078.1_g000005
rosa_roxburghii	Rroxscaffold_2G00081570
rosa_rugosa	Rorug02G0545900.1
rosa_samantha	Rh2AG618000 Rh2BG629500 Rh2CG598900 Rh2DG641300
rosa_wichuraiana	Rw2G051170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2124
Acc36I	ACCTGC	2 cut(s)	2124, 2237
AccB1I	GGYRCC	1 cut(s)	560
AccB7I	CCANNNNNTGG	3 cut(s)	2221, 2276, 2323
AccBSI	CCGCTC	2 cut(s)	285, 315
AclWI	GGATC	7 cut(s)	568, 729, 1217, 1230, 1735, 1741, 2417
AcoI	YGGCCR	5 cut(s)	3, 622, 809, 1858, 1972
AcsI	RAATTY	3 cut(s)	804, 1376, 2511
AcuI	CTGAAG	1 cut(s)	1248
AfaI	GTAC	4 cut(s)	146, 1129, 2402, 2697
AfiI	CCNNNNNNNGG	6 cut(s)	541, 613, 1570, 2221, 2276, 2323
AflIII	ACRYGT	3 cut(s)	1365, 1431, 2692
AgsI	TTSAA	8 cut(s)	226, 761, 977, 1089, 1237, 1247, 1656, 2240
AjnI	CCWGG	2 cut(s)	576, 1563
AjuI	GAANNNNNNNTTGG	2 cut(s)	997, 1029
AluBI	AGCT	5 cut(s)	980, 2208, 2291, 2546, 2708
AluI	AGCT	5 cut(s)	980, 2208, 2291, 2546, 2708
Alw21I	GWGCWC	1 cut(s)	2524
Alw26I	GTCTC	4 cut(s)	53, 361, 2653, 2692
AlwI	GGATC	7 cut(s)	568, 729, 1217, 1230, 1735, 1741, 2417
AoxI	GGCC	8 cut(s)	3, 38, 603, 622, 809, 1016, 1858, 1972
ApeKI	GCWGC	5 cut(s)	237, 1382, 1448, 2669, 2672
ApoI	RAATTY	3 cut(s)	804, 1376, 2511
ArsI	GACNNNNNNTTYG	2 cut(s)	2697, 2729
AspLEI	GCGC	1 cut(s)	547
AspS9I	GGNCC	4 cut(s)	604, 657, 667, 1615
AsuC2I	CCSGG	1 cut(s)	607
AsuHPI	GGTGA	8 cut(s)	35, 370, 391, 422, 454, 1748, 1937, 2036
AvaII	GGWCC	3 cut(s)	657, 667, 1615
AxyI	CCTNAGG	1 cut(s)	1620
BalI	TGGCCA	4 cut(s)	624, 811, 1860, 1974
BamHI	GGATCC	1 cut(s)	1222
BanI	GGYRCC	1 cut(s)	560
BauI	CACGAG	2 cut(s)	1700, 2525
BbsI	GAAGAC	3 cut(s)	517, 1254, 1842
Bbv12I	GWGCWC	1 cut(s)	2524
BbvI	GCAGC	5 cut(s)	249, 1369, 1435, 2656, 2659
BceAI	ACGGC	1 cut(s)	503
BcgI	CGANNNNNNTGC	6 cut(s)	875, 909, 2285, 2319, 2651, 2685
BciT130I	CCWGG	2 cut(s)	578, 1565
BciVI	GTATCC	1 cut(s)	1355
BclI	TGATCA	5 cut(s)	468, 1350, 1942, 1954, 2023
BcnI	CCSGG	1 cut(s)	607
BcoDI	GTCTC	4 cut(s)	53, 361, 2653, 2692
BfaI	CTAG	2 cut(s)	138, 1635
BfoI	RGCGCY	1 cut(s)	548
BfuAI	ACCTGC	2 cut(s)	2124, 2237
BfuI	GTATCC	1 cut(s)	1355
BisI	GCNGC	9 cut(s)	238, 559, 567, 603, 643, 1383, 1449, 2670, 2673
BlpI	GCTNAGC	1 cut(s)	2292
BlsI	GCNGC	9 cut(s)	239, 560, 568, 604, 644, 1384, 1450, 2671, 2674
Bme1390I	CCNGG	3 cut(s)	578, 607, 1565
Bme18I	GGWCC	3 cut(s)	657, 667, 1615
BmgT120I	GGNCC	4 cut(s)	604, 657, 667, 1615
BmiI	GGNNCC	3 cut(s)	562, 1224, 1617
BmrFI	CCNGG	3 cut(s)	578, 607, 1565
BmrI	ACTGGG	1 cut(s)	1259
BmsI	GCATC	5 cut(s)	807, 1411, 1717, 1771, 2305
BmuI	ACTGGG	1 cut(s)	1259
BpiI	GAAGAC	3 cut(s)	517, 1254, 1842
BplI	GAGNNNNNCTC	2 cut(s)	2530, 2562
BpmI	CTGGAG	1 cut(s)	2730
Bpu1102I	GCTNAGC	1 cut(s)	2292
BpuMI	CCSGG	1 cut(s)	607
Bsa29I	ATCGAT	2 cut(s)	506, 863
BsaAI	YACGTR	1 cut(s)	2695
BsaBI	GATNNNNATC	2 cut(s)	651, 1732
BsaI	GGTCTC	1 cut(s)	2692
BsaJI	CCNNGG	5 cut(s)	519, 663, 670, 1969, 2358
BsaWI	WCCGGW	1 cut(s)	534
BsaXI	ACNNNNNCTCC	4 cut(s)	978, 984, 1008, 1014
Bsc4I	CCNNNNNNNGG	6 cut(s)	541, 613, 1570, 2221, 2276, 2323
Bse1I	ACTGG	3 cut(s)	1254, 1363, 2597
Bse21I	CCTNAGG	1 cut(s)	1620
Bse3DI	GCAATG	1 cut(s)	2726
Bse8I	GATNNNNATC	2 cut(s)	651, 1732
BseBI	CCWGG	2 cut(s)	578, 1565
BseCI	ATCGAT	2 cut(s)	506, 863
BseDI	CCNNGG	5 cut(s)	519, 663, 670, 1969, 2358
BseGI	GGATG	5 cut(s)	203, 625, 1260, 1762, 2414
BseJI	GATNNNNATC	2 cut(s)	651, 1732
BseLI	CCNNNNNNNGG	6 cut(s)	541, 613, 1570, 2221, 2276, 2323
BseMI	GCAATG	1 cut(s)	2726
BseMII	CTCAG	2 cut(s)	1043, 1634
BseNI	ACTGG	3 cut(s)	1254, 1363, 2597
BseXI	GCAGC	5 cut(s)	249, 1369, 1435, 2656, 2659
BseYI	CCCAGC	3 cut(s)	60, 480, 1385
BsgI	GTGCAG	1 cut(s)	2102
BshFI	GGCC	8 cut(s)	5, 40, 605, 624, 811, 1018, 1860, 1974
BshNI	GGYRCC	1 cut(s)	560
BshVI	ATCGAT	2 cut(s)	506, 863
BsiHKAI	GWGCWC	1 cut(s)	2524
BsiSI	CCGG	3 cut(s)	535, 591, 607
BslFI	GGGAC	5 cut(s)	51, 463, 783, 1188, 2644
BslI	CCNNNNNNNGG	6 cut(s)	541, 613, 1570, 2221, 2276, 2323
BsmAI	GTCTC	4 cut(s)	53, 361, 2653, 2692
BsmBI	CGTCTC	2 cut(s)	53, 361
BsmFI	GGGAC	5 cut(s)	51, 463, 783, 1188, 2644
BsmI	GAATGC	3 cut(s)	1820, 2583, 2744
BsnI	GGCC	8 cut(s)	5, 40, 605, 624, 811, 1018, 1860, 1974
Bso31I	GGTCTC	1 cut(s)	2692
Bsp1286I	GDGCHC	1 cut(s)	2524
Bsp1720I	GCTNAGC	1 cut(s)	2292
Bsp19I	CCATGG	1 cut(s)	1969
BspANI	GGCC	8 cut(s)	5, 40, 605, 624, 811, 1018, 1860, 1974
BspCNI	CTCAG	2 cut(s)	1044, 1633
BspDI	ATCGAT	2 cut(s)	506, 863
BspHI	TCATGA	5 cut(s)	1392, 1789, 1864, 1876, 2074
BspLI	GGNNCC	3 cut(s)	562, 1224, 1617
BspMI	ACCTGC	2 cut(s)	2124, 2237
BspPI	GGATC	7 cut(s)	568, 729, 1217, 1230, 1735, 1741, 2417
BspT107I	GGYRCC	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	2692
BsrBI	CCGCTC	2 cut(s)	285, 315
BsrDI	GCAATG	1 cut(s)	2726
BsrI	ACTGG	3 cut(s)	1254, 1363, 2597
BssECI	CCNNGG	5 cut(s)	519, 663, 670, 1969, 2358
BssSI	CACGAG	2 cut(s)	1700, 2525
BssT1I	CCWWGG	3 cut(s)	663, 1969, 2358
Bst2BI	CACGAG	2 cut(s)	1700, 2525
Bst2UI	CCWGG	2 cut(s)	578, 1565
Bst4CI	ACNGT	9 cut(s)	47, 144, 222, 477, 780, 934, 1033, 1179, 1200
Bst6I	CTCTTC	5 cut(s)	266, 341, 394, 418, 1096
BstAPI	GCANNNNNTGC	1 cut(s)	1935
BstBAI	YACGTR	1 cut(s)	2695
BstC8I	GCNNGC	3 cut(s)	1490, 1494, 2431
BstDEI	CTNAG	4 cut(s)	1052, 1620, 2292, 2480
BstDSI	CCRYGG	3 cut(s)	519, 670, 1969
BstF5I	GGATG	5 cut(s)	203, 625, 1260, 1762, 2414
BstH2I	RGCGCY	1 cut(s)	548
BstHHI	GCGC	1 cut(s)	547
BstMAI	GTCTC	4 cut(s)	53, 361, 2653, 2692
BstMWI	GCNNNNNNNGC	7 cut(s)	113, 817, 1300, 1454, 1935, 2426, 2678
BstNI	CCWGG	2 cut(s)	578, 1565
BstNSI	RCATGY	2 cut(s)	1369, 1435
BstSCI	CCNGG	3 cut(s)	576, 605, 1563
BstV1I	GCAGC	5 cut(s)	249, 1369, 1435, 2656, 2659
BstV2I	GAAGAC	3 cut(s)	517, 1254, 1842
BstX2I	RGATCY	1 cut(s)	1222
BstXI	CCANNNNNNTGG	2 cut(s)	577, 2201
BstYI	RGATCY	1 cut(s)	1222
Bsu15I	ATCGAT	2 cut(s)	506, 863
Bsu36I	CCTNAGG	1 cut(s)	1620
BsuI	GTATCC	1 cut(s)	1355
BsuRI	GGCC	8 cut(s)	5, 40, 605, 624, 811, 1018, 1860, 1974
BsuTUI	ATCGAT	2 cut(s)	506, 863
BtgI	CCRYGG	3 cut(s)	519, 670, 1969
BtgZI	GCGATG	1 cut(s)	2288
BtsCI	GGATG	5 cut(s)	203, 625, 1260, 1762, 2414
BtsIMutI	CAGTG	3 cut(s)	776, 1005, 1837
BveI	ACCTGC	2 cut(s)	2124, 2237
Cac8I	GCNNGC	3 cut(s)	1490, 1494, 2431
CciI	TCATGA	5 cut(s)	1392, 1789, 1864, 1876, 2074
CfoI	GCGC	1 cut(s)	547
Cfr13I	GGNCC	4 cut(s)	604, 657, 667, 1615
ClaI	ATCGAT	2 cut(s)	506, 863
CseI	GACGC	1 cut(s)	53
Csp6I	GTAC	4 cut(s)	145, 1128, 2401, 2696
CspCI	CAANNNNNGTGG	2 cut(s)	652, 687
CviQI	GTAC	4 cut(s)	145, 1128, 2401, 2696
DdeI	CTNAG	4 cut(s)	1052, 1620, 2292, 2480
EaeI	YGGCCR	5 cut(s)	3, 622, 809, 1858, 1972
Eam1104I	CTCTTC	5 cut(s)	266, 341, 394, 418, 1096
EarI	CTCTTC	5 cut(s)	266, 341, 394, 418, 1096
EciI	GGCGGA	3 cut(s)	103, 632, 2240
Eco130I	CCWWGG	3 cut(s)	663, 1969, 2358
Eco147I	AGGCCT	1 cut(s)	40
Eco31I	GGTCTC	1 cut(s)	2692
Eco32I	GATATC	3 cut(s)	1416, 1629, 2382
Eco47I	GGWCC	3 cut(s)	657, 667, 1615
Eco57I	CTGAAG	1 cut(s)	1248
Eco81I	CCTNAGG	1 cut(s)	1620
EcoRI	GAATTC	1 cut(s)	2511
EcoRII	CCWGG	2 cut(s)	576, 1563
EcoRV	GATATC	3 cut(s)	1416, 1629, 2382
EcoT14I	CCWWGG	3 cut(s)	663, 1969, 2358
EcoT22I	ATGCAT	1 cut(s)	1820
ErhI	CCWWGG	3 cut(s)	663, 1969, 2358
Esp3I	CGTCTC	2 cut(s)	53, 361
FaqI	GGGAC	5 cut(s)	51, 463, 783, 1188, 2644
FauI	CCCGC	1 cut(s)	1767
FauNDI	CATATG	1 cut(s)	2600
FbaI	TGATCA	5 cut(s)	468, 1350, 1942, 1954, 2023
Fnu4HI	GCNGC	9 cut(s)	238, 559, 567, 603, 643, 1383, 1449, 2670, 2673
FokI	GGATG	5 cut(s)	190, 632, 1267, 1749, 2421
Fsp4HI	GCNGC	9 cut(s)	238, 559, 567, 603, 643, 1383, 1449, 2670, 2673
FspBI	CTAG	2 cut(s)	138, 1635
GlaI	GCGC	1 cut(s)	546
GluI	GCNGC	9 cut(s)	238, 559, 567, 603, 643, 1383, 1449, 2670, 2673
GsaI	CCCAGC	3 cut(s)	64, 484, 1389
GsuI	CTGGAG	1 cut(s)	2730
HaeII	RGCGCY	1 cut(s)	548
HaeIII	GGCC	8 cut(s)	5, 40, 605, 624, 811, 1018, 1860, 1974
HapII	CCGG	3 cut(s)	535, 591, 607
HgaI	GACGC	1 cut(s)	53
HhaI	GCGC	1 cut(s)	547
Hin6I	GCGC	1 cut(s)	545
HinP1I	GCGC	1 cut(s)	545
HindIII	AAGCTT	1 cut(s)	2206
HpaII	CCGG	3 cut(s)	535, 591, 607
HphI	GGTGA	8 cut(s)	35, 370, 391, 422, 454, 1748, 1937, 2036
Hpy166II	GTNNAC	3 cut(s)	154, 1128, 1275
Hpy188I	TCNGA	7 cut(s)	726, 1053, 1100, 1267, 1420, 2372, 2589
Hpy8I	GTNNAC	3 cut(s)	154, 1128, 1275
Hpy99I	CGWCG	1 cut(s)	2624
HpyAV	CCTTC	8 cut(s)	94, 118, 265, 333, 352, 379, 1787, 2321
HpyCH4III	ACNGT	9 cut(s)	47, 144, 222, 477, 780, 934, 1033, 1179, 1200
HpyCH4IV	ACGT	5 cut(s)	597, 1995, 2343, 2622, 2694
HpyCH4V	TGCA	9 cut(s)	179, 1337, 1457, 1496, 1818, 2119, 2341, 2675, 2731
HpyF10VI	GCNNNNNNNGC	7 cut(s)	113, 817, 1300, 1454, 1935, 2426, 2678
HpyF3I	CTNAG	4 cut(s)	1052, 1620, 2292, 2480
HpySE526I	ACGT	5 cut(s)	597, 1995, 2343, 2622, 2694
HspAI	GCGC	1 cut(s)	545
Ksp22I	TGATCA	5 cut(s)	468, 1350, 1942, 1954, 2023
LmnI	GCTCC	5 cut(s)	290, 320, 1705, 1745, 2508
Lsp1109I	GCAGC	5 cut(s)	249, 1369, 1435, 2656, 2659
LweI	GCATC	5 cut(s)	807, 1411, 1717, 1771, 2305
MaeI	CTAG	2 cut(s)	138, 1635
MaeII	ACGT	5 cut(s)	597, 1995, 2343, 2622, 2694
MaeIII	GTNAC	4 cut(s)	1114, 1194, 1323, 1427
MbiI	CCGCTC	2 cut(s)	285, 315
MfeI	CAATTG	5 cut(s)	793, 887, 1458, 1918, 2271
MflI	RGATCY	1 cut(s)	1222
MhlI	GDGCHC	1 cut(s)	2524
MlsI	TGGCCA	4 cut(s)	624, 811, 1860, 1974
MluNI	TGGCCA	4 cut(s)	624, 811, 1860, 1974
MlyI	GAGTC	6 cut(s)	798, 1268, 1604, 1766, 2626, 2721
MmeI	TCCRAC	5 cut(s)	1119, 1555, 2350, 2715, 2725
Mox20I	TGGCCA	4 cut(s)	624, 811, 1860, 1974
Mph1103I	ATGCAT	1 cut(s)	1820
MscI	TGGCCA	4 cut(s)	624, 811, 1860, 1974
MseI	TTAA	5 cut(s)	1299, 1674, 1688, 2397, 2723
Msp20I	TGGCCA	4 cut(s)	624, 811, 1860, 1974
MspI	CCGG	3 cut(s)	535, 591, 607
MspR9I	CCNGG	3 cut(s)	578, 607, 1565
MunI	CAATTG	5 cut(s)	793, 887, 1458, 1918, 2271
Mva1269I	GAATGC	3 cut(s)	1820, 2583, 2744
MvaI	CCWGG	2 cut(s)	578, 1565
MwoI	GCNNNNNNNGC	7 cut(s)	113, 817, 1300, 1454, 1935, 2426, 2678
NciI	CCSGG	1 cut(s)	607
NcoI	CCATGG	1 cut(s)	1969
NdeI	CATATG	1 cut(s)	2600
NlaIV	GGNNCC	3 cut(s)	562, 1224, 1617
NmeAIII	GCCGAG	1 cut(s)	2468
NmuCI	GTSAC	2 cut(s)	1323, 1427
NsiI	ATGCAT	1 cut(s)	1820
NspI	RCATGY	2 cut(s)	1369, 1435
PagI	TCATGA	5 cut(s)	1392, 1789, 1864, 1876, 2074
PaqCI	CACCTGC	1 cut(s)	2124
PceI	AGGCCT	1 cut(s)	40
PciI	ACATGT	2 cut(s)	1365, 1431
PcsI	WCGNNNNNNNCGW	1 cut(s)	2700
PctI	GAATGC	3 cut(s)	1820, 2583, 2744
PfeI	GAWTC	6 cut(s)	489, 508, 629, 652, 1268, 2228
PflMI	CCANNNNNTGG	3 cut(s)	2221, 2276, 2323
PkrI	GCNGC	9 cut(s)	239, 560, 568, 604, 644, 1384, 1450, 2671, 2674
PleI	GAGTC	6 cut(s)	797, 1267, 1603, 1766, 2626, 2720
PpsI	GAGTC	6 cut(s)	797, 1267, 1603, 1766, 2626, 2720
Ppu21I	YACGTR	1 cut(s)	2695
PscI	ACATGT	2 cut(s)	1365, 1431
Psp6I	CCWGG	2 cut(s)	576, 1563
PspFI	CCCAGC	3 cut(s)	60, 480, 1385
PspGI	CCWGG	2 cut(s)	576, 1563
PspN4I	GGNNCC	3 cut(s)	562, 1224, 1617
PspPI	GGNCC	4 cut(s)	604, 657, 667, 1615
PsuI	RGATCY	1 cut(s)	1222
RsaI	GTAC	4 cut(s)	146, 1129, 2402, 2697
RsaNI	GTAC	4 cut(s)	145, 1128, 2401, 2696
SaqAI	TTAA	5 cut(s)	1299, 1674, 1688, 2397, 2723
SatI	GCNGC	9 cut(s)	238, 559, 567, 603, 643, 1383, 1449, 2670, 2673
Sau96I	GGNCC	4 cut(s)	604, 657, 667, 1615
SchI	GAGTC	6 cut(s)	798, 1268, 1604, 1766, 2626, 2721
ScrFI	CCNGG	3 cut(s)	578, 607, 1565
SduI	GDGCHC	1 cut(s)	2524
SfaNI	GCATC	5 cut(s)	807, 1411, 1717, 1771, 2305
SinI	GGWCC	3 cut(s)	657, 667, 1615
SseBI	AGGCCT	1 cut(s)	40
SspI	AATATT	1 cut(s)	2334
SspMI	CTAG	2 cut(s)	138, 1635
StuI	AGGCCT	1 cut(s)	40
StyD4I	CCNGG	3 cut(s)	576, 605, 1563
StyI	CCWWGG	3 cut(s)	663, 1969, 2358
TaaI	ACNGT	9 cut(s)	47, 144, 222, 477, 780, 934, 1033, 1179, 1200
TaiI	ACGT	5 cut(s)	600, 1998, 2346, 2625, 2697
TaqI	TCGA	9 cut(s)	167, 506, 632, 863, 895, 1631, 2435, 2553, 2661
TaqII	GACCGA	1 cut(s)	645
TatI	WGTACW	2 cut(s)	1127, 2400
TauI	GCSGC	4 cut(s)	561, 569, 605, 645
TfiI	GAWTC	6 cut(s)	489, 508, 629, 652, 1268, 2228
Tru1I	TTAA	5 cut(s)	1299, 1674, 1688, 2397, 2723
Tru9I	TTAA	5 cut(s)	1299, 1674, 1688, 2397, 2723
TscAI	CASTG	3 cut(s)	783, 1005, 1837
TseFI	GTSAC	2 cut(s)	1323, 1427
TseI	GCWGC	5 cut(s)	237, 1382, 1448, 2669, 2672
Tsp45I	GTSAC	2 cut(s)	1323, 1427
TspGWI	ACGGA	3 cut(s)	174, 659, 2437
TspRI	CASTG	3 cut(s)	783, 1005, 1837
Van91I	CCANNNNNTGG	3 cut(s)	2221, 2276, 2323
VpaK11BI	GGWCC	3 cut(s)	657, 667, 1615
XapI	RAATTY	3 cut(s)	804, 1376, 2511
XbaI	TCTAGA	1 cut(s)	137
XceI	RCATGY	2 cut(s)	1369, 1435
XspI	CTAG	2 cut(s)	138, 1635
Zsp2I	ATGCAT	1 cut(s)	1820

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.