Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0015244

Physical Location & Seq

Genomic Coordinates Reverse (-)

78838688 .. 78841650

Gene Structure 2963 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G051170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2580 bp

ATGGCCGATCACCACCACCACCACCACCGCCCCCACCGCCTCTCCCTCCCTCAACGCCCCACCACCCCCACCACCTTCGCCTCCACCACCCCCACCGCCTCCCGCCAATACCCACTCTACCCCTTCACTCCCTCCGCCGCCACTCCTAAACACCGCCTCTCTTCTCTCAACCCCAAACCCCTCTCCGCCGGCGCCGGCGCCGGAAGCAAATCCTCTCTCTCATTCCTCTTCCTCCTCCTCCTCTCTCTCCGATCACTCTACTCCCTCCTCCCCTTCCTCCGCTCCTCCCCTTCCTTCTCTCTCTTCCCCTTCTCCTTCCTCGTCTCCCTTCTCTCCTTCCTCCTAACCCTAGCTTTCTCTTTCTTCACCTCCTCCGCCGCCTCCAAAGACCCGTTCCACCAGAAGCCCAACCAGCCCATCTTCTCCCTCACTCTCATCACTCAATCCCAGCACAGACTCCTCGTCGCGAAATCGATTCTTCTCGCCGGAGTCTTCCTCCTCCGCTTCCAAGCCCTCCGGTACTGCGGCACCGCCGCCATGATCCTAGCCGAGATGTCTGGAAATGTGGCGGCAAGGTTCTTGGCGGAGGGGAGGAAACAGAGTGTGGTGGGCGGCGGTGGTGATCGGAATCGGAGCTCCAAGGTTCGTGGGTTTCTTGCTCTGTTTACTGGGTTGTTCTTGTTATCTATGAGCTGGGATCGGATTGAATGCTTTCCGTTTTCGGCCAAGTTTGTGCAAAAAATGGGAGTGTCTGTGTTGCCTAGAGTGGATTGTGTGAGGATTTGGCCAATGCTGCTTCCGTTTCTGTCTGGATTTTTGGGGTGTTATGAGAGGGTTTCGATGAATTGGGGGACTATTAAGCAGCTGGGGAGGAAGAGGGTTCGGGTGATTTCGTTGTTTTTTACTACTGTTGTGCTGTTCATTCCTGCTGTTGTTAGCGTTTTCATGTTTGAAGCTGAGGGAGATGGAGTTGTTTCCATTGGGAACTTGGGCTGGCCTCTGGCAAACACTGTTGTTTTCGGTGTGCTTCTGAGTGAGAGTTATAGTGATGAGAAGTTAATGAGTTCTAAAGATTTTCGGAGGGAGTATTTGGTGACTTTCTTATGTACTGTTGTATTGGAGCTGTTCTATTTTGCTGAATTGTCGCTTTGGGGGCTATTGCTTTGTGGTATGTTGCTGTACGTTGCTGTTAGGGAGTTGGATCCTTTTCATGCGAGTTATGTTGAACTGGGAATGGAGTCGTCGGAATCATTTAGTGCATCCGTTATGAAACCTATTAGGCACATTTTGAGTGAGAGGAAATCGCGGAAGATTGCACTTTTCCTTCTGATCAATACTGCATACATGTTTGTGGAATTCATTGCTGGGTTCATGAGCAATAGTCTAGGGCTGATATCAGATGCCTGTCACATGTTGTTTGATTGCGCTGCTCTGGCAATTGGGCTATATGCTTCGTATATTTCGCGCTTGCCTGCAAATAATCAGTTTAACTATGGCCGTGGGAGATTTGAGGTTCTTGCAGGATATACAAATGCTGTTTTCCTGGTTTTGGTTGGAGCATTAATAGTATTGGAATCACTTGAGAGGATTTTGGACCCTCAGGAGATGTCAACTAGCAGTATACTAGTTGTTTCAATTGGAGGGCTTCTTGTCAATGTGGTTGGTTTAATCTTCTTTCATGAGGAGCATCATCATGCCCATGGTGGATCCGGATCATGCTCTCACTCCCACAGTCATCAACATTCGCATGACTCTGTTGGACATAATCATGCAGGTCATGAAAAGCTTGAGGAATCATGTATAGCTATTTCTCATGAATGCCATGAAGAATCGTGTGCCAGCCATGATGACCATCACCAACATGGCAGTTCTGTTGGCTGCAAAGATCACCGGCACGATCACCATGACCACAGTCACCAGCATGATCACCATGACCACAGCCACCAGCATGATCACCAGCATGACCACCATGACCACAGCCACCAGCATGACCACCATGATCACAGCCATCATCATGACCACCATGGCCACAGTCACCAGCATGACAACCATGGCCACAACCACCAGCATGGTGGTTCCGAGCTGAAAAGACTGCCAACCGGTGGAGAAAAATCAACTAAGCAACAGCATCACCACATTGACCACAACATGGAAGGAATATTTCTGCATGTTTTGGCTGACACCTTAGGGAGTGTCGGAGTTGTTATATCAACACTCTTAATTAAGTACAAGGGATGGCTTGTTGCTGATCCTGCATGCTCGATATTTATTTCTGTCTTGATTATATCTTCAGTTATCCCATTGCTCAGGAACTCTGCAGAAGTCTTGCTCCAAAGAATTCCGAGGGCACATGAGCAGGATTTGAGAAAAGCTCTTAATGATGTTAGGAAGATAAGGGGGGTTTCTGGTATTCGGAACTTGCATGTCTGGAGTTTCACAAACTCAGATGTAGTGGGTACGCTCCATCTTCATGTTTCCACAGAAGTTGACAAAGGATCTGCAAAAGCTCAGGTGTCGCACTTATTACATGAAGCGGGAATCAAGGATTTAACGGTGCAGTTGGAATGTGTTGAGGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0071578 GO:0072509 GO:0072511 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098739 GO:0099587

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

859

Amino Acids

95.65

Weight (kDa)

7.25

Isoelectric Point (pI)

38.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	439 - 571	3.3e-33	Cation efflux transmembrane domain
Cation_efflux	PF01545	707 - 777	1.6e-13	Cation efflux transmembrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04620 AT2G04620
fragaria_vesca	FvH4_6g49950
malus_domestica	MD17G1042100.v1.1
prunus_persica	Prupe.3G277100_v2.0.a1
pyrus_communis	pycom17g03760
rosa_chinensis	RchiOBHm_Chr2g0170121
rosa_laevigata	RLG00000021925
rosa_multiflora	Rmu_sc0013078.1_g000005
rosa_roxburghii	Rroxscaffold_2G00081570
rosa_rugosa	Rorug02G0545900.1
rosa_samantha	Rh2AG618000 Rh2BG629500 Rh2CG598900 Rh2DG641300
rosa_wichuraiana	Rw2G051170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1763
AccB1I	GGYRCC	3 cut(s)	191, 197, 527
AccB7I	CCANNNNNTGG	2 cut(s)	2102, 2149
AccBSI	CCGCTC	1 cut(s)	282
AccI	GTMKAC	1 cut(s)	1621
AccII	CGCG	3 cut(s)	467, 1306, 1465
AccIII	TCCGGA	1 cut(s)	1709
AclWI	GGATC	9 cut(s)	535, 705, 1196, 1209, 1701, 1714, 1720, 2243, 2503
AcoI	YGGCCR	6 cut(s)	3, 723, 785, 1495, 2026, 2053
AcsI	RAATTY	2 cut(s)	1355, 2337
AcuI	CTGAAG	1 cut(s)	2274
AcyI	GRCGYC	2 cut(s)	192, 198
AfaI	GTAC	5 cut(s)	521, 1108, 1181, 2228, 2458
AfiI	CCNNNNNNNGG	3 cut(s)	1549, 2102, 2149
AflIII	ACRYGT	2 cut(s)	1344, 1410
AgeI	ACCGGT	1 cut(s)	2099
AgsI	TTSAA	4 cut(s)	707, 953, 1226, 1635
AhdI	GACNNNNNGTC	1 cut(s)	461
AhlI	ACTAGT	1 cut(s)	1624
AjnI	CCWGG	1 cut(s)	1542
AjuI	GAANNNNNNNTTGG	2 cut(s)	377, 409
Alw21I	GWGCWC	1 cut(s)	638
Alw26I	GTCTC	1 cut(s)	328
AlwI	GGATC	9 cut(s)	535, 705, 1196, 1209, 1701, 1714, 1720, 2243, 2503
Aor13HI	TCCGGA	1 cut(s)	1709
AoxI	GGCC	7 cut(s)	3, 723, 785, 995, 1495, 2026, 2053
ApeKI	GCWGC	4 cut(s)	793, 862, 1427, 1878
ApoI	RAATTY	2 cut(s)	1355, 2337
AseI	ATTAAT	1 cut(s)	1562
AsiGI	ACCGGT	1 cut(s)	2099
Asp700I	GAANNNNTTC	1 cut(s)	711
AspLEI	GCGC	4 cut(s)	194, 200, 1427, 1467
AspS9I	GGNCC	1 cut(s)	1594
AvaII	GGWCC	1 cut(s)	1594
AxyI	CCTNAGG	2 cut(s)	1599, 2185
BaeGI	GKGCMC	1 cut(s)	2350
BalI	TGGCCA	3 cut(s)	787, 2028, 2055
BamHI	GGATCC	2 cut(s)	1201, 1706
BanI	GGYRCC	3 cut(s)	191, 197, 527
BanII	GRGCYC	1 cut(s)	638
BbsI	GAAGAC	1 cut(s)	484
Bbv12I	GWGCWC	1 cut(s)	638
BbvCI	CCTCAGC	1 cut(s)	957
BbvI	GCAGC	4 cut(s)	780, 874, 1414, 1865
BccI	CCATC	6 cut(s)	425, 959, 1860, 2016, 2229, 2472
BceAI	ACGGC	1 cut(s)	1482
BciT130I	CCWGG	1 cut(s)	1544
BclI	TGATCA	4 cut(s)	1329, 1924, 1951, 1999
BcoDI	GTCTC	1 cut(s)	328
BcuI	ACTAGT	1 cut(s)	1624
BfaI	CTAG	6 cut(s)	350, 545, 762, 1385, 1614, 1625
BfmI	CTRYAG	1 cut(s)	2316
BfoI	RGCGCY	2 cut(s)	195, 201
BfuAI	ACCTGC	1 cut(s)	1763
Bme1390I	CCNGG	1 cut(s)	1544
Bme18I	GGWCC	1 cut(s)	1594
BmeRI	GACNNNNNGTC	1 cut(s)	461
BmgT120I	GGNCC	1 cut(s)	1594
BmiI	GGNNCC	7 cut(s)	193, 199, 529, 1203, 1596, 1708, 2077
BmrFI	CCNGG	1 cut(s)	1544
BmrI	ACTGGG	2 cut(s)	678, 1238
BmsI	GCATC	4 cut(s)	1268, 1390, 1696, 2137
BmuI	ACTGGG	2 cut(s)	678, 1238
BoxI	GACNNNNGTC	1 cut(s)	1911
BpiI	GAAGAC	1 cut(s)	484
BplI	GAGNNNNNCTC	4 cut(s)	480, 512, 2356, 2388
BpmI	CTGGAG	1 cut(s)	2449
Bpu10I	CCTNAGC	3 cut(s)	957, 2306, 2508
BpuEI	CTTGAG	2 cut(s)	1601, 1808
Bsa29I	ATCGAT	1 cut(s)	473
BsaBI	GATNNNNATC	3 cut(s)	627, 1711, 1851
BsaHI	GRCGYC	2 cut(s)	192, 198
BsaJI	CCNNGG	6 cut(s)	639, 1498, 1699, 2023, 2050, 2342
BsaWI	WCCGGW	3 cut(s)	516, 1709, 2099
BsaXI	ACNNNNNCTCC	6 cut(s)	26, 56, 738, 768, 954, 984
Bsc4I	CCNNNNNNNGG	3 cut(s)	1549, 2102, 2149
Bse118I	RCCGGY	4 cut(s)	188, 194, 1890, 2099
Bse1I	ACTGG	2 cut(s)	673, 1233
Bse21I	CCTNAGG	2 cut(s)	1599, 2185
Bse3DI	GCAATG	2 cut(s)	1359, 2300
Bse8I	GATNNNNATC	3 cut(s)	627, 1711, 1851
BseAI	TCCGGA	1 cut(s)	1709
BseBI	CCWGG	1 cut(s)	1544
BseCI	ATCGAT	1 cut(s)	473
BseDI	CCNNGG	6 cut(s)	639, 1498, 1699, 2023, 2050, 2342
BseGI	GGATG	2 cut(s)	1259, 2240
BseJI	GATNNNNATC	3 cut(s)	627, 1711, 1851
BseLI	CCNNNNNNNGG	3 cut(s)	1549, 2102, 2149
BseMI	GCAATG	2 cut(s)	1359, 2300
BseMII	CTCAG	6 cut(s)	948, 1022, 1613, 2320, 2457, 2522
BseNI	ACTGG	2 cut(s)	673, 1233
BseRI	GAGGAG	9 cut(s)	224, 227, 230, 257, 274, 361, 449, 488, 1697
BseSI	GKGCMC	1 cut(s)	2350
BseXI	GCAGC	4 cut(s)	780, 874, 1414, 1865
BseYI	CCCAGC	4 cut(s)	447, 693, 865, 1364
BsgI	GTGCAG	1 cut(s)	2576
Bsh1236I	CGCG	3 cut(s)	467, 1306, 1465
BshFI	GGCC	7 cut(s)	5, 725, 787, 997, 1497, 2028, 2055
BshNI	GGYRCC	3 cut(s)	191, 197, 527
BshTI	ACCGGT	1 cut(s)	2099
BshVI	ATCGAT	1 cut(s)	473
BsiHKAI	GWGCWC	1 cut(s)	638
BsiSI	CCGG	8 cut(s)	189, 195, 201, 486, 517, 1710, 1891, 2100
BslFI	GGGAC	1 cut(s)	865
BslI	CCNNNNNNNGG	3 cut(s)	1549, 2102, 2149
BsmAI	GTCTC	1 cut(s)	328
BsmBI	CGTCTC	1 cut(s)	328
BsmFI	GGGAC	1 cut(s)	865
BsmI	GAATGC	2 cut(s)	713, 1823
BsnI	GGCC	7 cut(s)	5, 725, 787, 997, 1497, 2028, 2055
Bsp1286I	GDGCHC	2 cut(s)	638, 2350
Bsp13I	TCCGGA	1 cut(s)	1709
Bsp19I	CCATGG	3 cut(s)	1699, 2023, 2050
Bsp68I	TCGCGA	1 cut(s)	467
BspANI	GGCC	7 cut(s)	5, 725, 787, 997, 1497, 2028, 2055
BspCNI	CTCAG	6 cut(s)	949, 1023, 1612, 2319, 2456, 2521
BspDI	ATCGAT	1 cut(s)	473
BspEI	TCCGGA	1 cut(s)	1709
BspFNI	CGCG	3 cut(s)	467, 1306, 1465
BspHI	TCATGA	5 cut(s)	1371, 1678, 1777, 1813, 2014
BspLI	GGNNCC	7 cut(s)	193, 199, 529, 1203, 1596, 1708, 2077
BspMAI	CTGCAG	1 cut(s)	2320
BspMI	ACCTGC	1 cut(s)	1763
BspPI	GGATC	9 cut(s)	535, 705, 1196, 1209, 1701, 1714, 1720, 2243, 2503
BspT107I	GGYRCC	3 cut(s)	191, 197, 527
BsrBI	CCGCTC	1 cut(s)	282
BsrDI	GCAATG	2 cut(s)	1359, 2300
BsrFI	RCCGGY	4 cut(s)	188, 194, 1890, 2099
BsrI	ACTGG	2 cut(s)	673, 1233
BssAI	RCCGGY	4 cut(s)	188, 194, 1890, 2099
BssECI	CCNNGG	6 cut(s)	639, 1498, 1699, 2023, 2050, 2342
BssNAI	GTATAC	1 cut(s)	1622
BssNI	GRCGYC	2 cut(s)	192, 198
BssT1I	CCWWGG	4 cut(s)	639, 1699, 2023, 2050
Bst1107I	GTATAC	1 cut(s)	1622
Bst2UI	CCWGG	1 cut(s)	1544
Bst4CI	ACNGT	7 cut(s)	910, 1012, 1111, 1733, 1913, 2033, 2554
Bst6I	CTCTTC	4 cut(s)	166, 233, 308, 869
BstACI	GRCGYC	2 cut(s)	192, 198
BstC8I	GCNNGC	7 cut(s)	190, 196, 995, 1469, 1473, 1840, 2257
BstDEI	CTNAG	8 cut(s)	957, 1031, 1599, 2118, 2185, 2306, 2443, 2508
BstDSI	CCRYGG	4 cut(s)	1498, 1699, 2023, 2050
BstF5I	GGATG	2 cut(s)	1259, 2240
BstFNI	CGCG	3 cut(s)	467, 1306, 1465
BstH2I	RGCGCY	2 cut(s)	195, 201
BstHHI	GCGC	4 cut(s)	194, 200, 1427, 1467
BstMAI	GTCTC	1 cut(s)	328
BstMWI	GCNNNNNNNGC	7 cut(s)	36, 204, 412, 793, 1153, 1433, 2252
BstNI	CCWGG	1 cut(s)	1544
BstNSI	RCATGY	5 cut(s)	1348, 1414, 2171, 2259, 2426
BstPAI	GACNNNNGTC	1 cut(s)	1911
BstSCI	CCNGG	1 cut(s)	1542
BstSFI	CTRYAG	1 cut(s)	2316
BstSLI	GKGCMC	1 cut(s)	2350
BstUI	CGCG	3 cut(s)	467, 1306, 1465
BstV1I	GCAGC	4 cut(s)	780, 874, 1414, 1865
BstV2I	GAAGAC	1 cut(s)	484
BstX2I	RGATCY	3 cut(s)	1201, 1706, 2495
BstXI	CCANNNNNNTGG	2 cut(s)	2069, 2072
BstYI	RGATCY	3 cut(s)	1201, 1706, 2495
BstZ17I	GTATAC	1 cut(s)	1622
Bsu15I	ATCGAT	1 cut(s)	473
Bsu36I	CCTNAGG	2 cut(s)	1599, 2185
BsuRI	GGCC	7 cut(s)	5, 725, 787, 997, 1497, 2028, 2055
BsuTUI	ATCGAT	1 cut(s)	473
BtgI	CCRYGG	4 cut(s)	1498, 1699, 2023, 2050
BtsCI	GGATG	2 cut(s)	1259, 2240
BtsIMutI	CAGTG	1 cut(s)	1008
BtuMI	TCGCGA	1 cut(s)	467
BveI	ACCTGC	1 cut(s)	1763
Cac8I	GCNNGC	7 cut(s)	190, 196, 995, 1469, 1473, 1840, 2257
CciI	TCATGA	5 cut(s)	1371, 1678, 1777, 1813, 2014
CfoI	GCGC	4 cut(s)	194, 200, 1427, 1467
Cfr10I	RCCGGY	4 cut(s)	188, 194, 1890, 2099
Cfr13I	GGNCC	1 cut(s)	1594
ClaI	ATCGAT	1 cut(s)	473
Csp6I	GTAC	5 cut(s)	520, 1107, 1180, 2227, 2457
CspAI	ACCGGT	1 cut(s)	2099
CspCI	CAANNNNNGTGG	4 cut(s)	628, 663, 2467, 2502
CviQI	GTAC	5 cut(s)	520, 1107, 1180, 2227, 2457
DdeI	CTNAG	8 cut(s)	957, 1031, 1599, 2118, 2185, 2306, 2443, 2508
DinI	GGCGCC	2 cut(s)	193, 199
DriI	GACNNNNNGTC	1 cut(s)	461
EaeI	YGGCCR	6 cut(s)	3, 723, 785, 1495, 2026, 2053
Eam1104I	CTCTTC	4 cut(s)	166, 233, 308, 869
Eam1105I	GACNNNNNGTC	1 cut(s)	461
EarI	CTCTTC	4 cut(s)	166, 233, 308, 869
EciI	GGCGGA	4 cut(s)	124, 175, 364, 599
Ecl136II	GAGCTC	1 cut(s)	636
Eco130I	CCWWGG	4 cut(s)	639, 1699, 2023, 2050
Eco24I	GRGCYC	1 cut(s)	638
Eco32I	GATATC	1 cut(s)	1395
Eco47I	GGWCC	1 cut(s)	1594
Eco53kI	GAGCTC	1 cut(s)	636
Eco57I	CTGAAG	1 cut(s)	2274
Eco81I	CCTNAGG	2 cut(s)	1599, 2185
EcoICRI	GAGCTC	1 cut(s)	636
EcoRI	GAATTC	2 cut(s)	1355, 2337
EcoRII	CCWGG	1 cut(s)	1542
EcoRV	GATATC	1 cut(s)	1395
EcoT14I	CCWWGG	4 cut(s)	639, 1699, 2023, 2050
EcoT38I	GRGCYC	1 cut(s)	638
EgeI	GGCGCC	2 cut(s)	193, 199
EheI	GGCGCC	2 cut(s)	193, 199
ErhI	CCWWGG	4 cut(s)	639, 1699, 2023, 2050
Esp3I	CGTCTC	1 cut(s)	328
FaqI	GGGAC	1 cut(s)	865
FauI	CCCGC	2 cut(s)	110, 2527
FbaI	TGATCA	4 cut(s)	1329, 1924, 1951, 1999
FblI	GTMKAC	1 cut(s)	1621
FokI	GGATG	2 cut(s)	1246, 2247
FriOI	GRGCYC	1 cut(s)	638
FspBI	CTAG	6 cut(s)	350, 545, 762, 1385, 1614, 1625
GlaI	GCGC	4 cut(s)	193, 199, 1426, 1466
GsaI	CCCAGC	4 cut(s)	451, 697, 869, 1368
GsuI	CTGGAG	1 cut(s)	2449
HaeII	RGCGCY	2 cut(s)	195, 201
HaeIII	GGCC	7 cut(s)	5, 725, 787, 997, 1497, 2028, 2055
HapII	CCGG	8 cut(s)	189, 195, 201, 486, 517, 1710, 1891, 2100
HhaI	GCGC	4 cut(s)	194, 200, 1427, 1467
Hin1I	GRCGYC	2 cut(s)	192, 198
Hin6I	GCGC	4 cut(s)	192, 198, 1425, 1465
HinP1I	GCGC	4 cut(s)	192, 198, 1425, 1465
HincII	GTYRAC	2 cut(s)	1611, 2488
HindII	GTYRAC	2 cut(s)	1611, 2488
HindIII	AAGCTT	1 cut(s)	1784
HpaII	CCGG	8 cut(s)	189, 195, 201, 486, 517, 1710, 1891, 2100
Hpy166II	GTNNAC	4 cut(s)	666, 1611, 1622, 2488
Hpy8I	GTNNAC	4 cut(s)	666, 1611, 1622, 2488
Hpy99I	CGWCG	2 cut(s)	467, 1246
HpyCH4III	ACNGT	7 cut(s)	910, 1012, 1111, 1733, 1913, 2033, 2554
HpyCH4IV	ACGT	1 cut(s)	1182
HpyF10VI	GCNNNNNNNGC	7 cut(s)	36, 204, 412, 793, 1153, 1433, 2252
HpyF3I	CTNAG	8 cut(s)	957, 1031, 1599, 2118, 2185, 2306, 2443, 2508
HpySE526I	ACGT	1 cut(s)	1182
Hsp92I	GRCGYC	2 cut(s)	192, 198
HspAI	GCGC	4 cut(s)	192, 198, 1425, 1465
KasI	GGCGCC	2 cut(s)	191, 197
Kpn2I	TCCGGA	1 cut(s)	1709
KroI	GCCGGC	2 cut(s)	188, 194
KroNI	GCCGGC	2 cut(s)	190, 196
Ksp22I	TGATCA	4 cut(s)	1329, 1924, 1951, 1999
LmnI	GCTCC	8 cut(s)	287, 633, 641, 1120, 1556, 1684, 2334, 2466
Lsp1109I	GCAGC	4 cut(s)	780, 874, 1414, 1865
LweI	GCATC	4 cut(s)	1268, 1390, 1696, 2137
MaeI	CTAG	6 cut(s)	350, 545, 762, 1385, 1614, 1625
MaeII	ACGT	1 cut(s)	1182
MaeIII	GTNAC	4 cut(s)	1093, 1406, 1913, 2033
MbiI	CCGCTC	1 cut(s)	282
MfeI	CAATTG	2 cut(s)	1437, 1635
MflI	RGATCY	3 cut(s)	1201, 1706, 2495
MhlI	GDGCHC	2 cut(s)	638, 2350
MlsI	TGGCCA	3 cut(s)	787, 2028, 2055
MluCI	AATT	7 cut(s)	844, 1139, 1355, 1437, 1635, 2220, 2337
MluNI	TGGCCA	3 cut(s)	787, 2028, 2055
Mly113I	GGCGCC	2 cut(s)	192, 198
MlyI	GAGTC	4 cut(s)	450, 498, 1247, 1745
MmeI	TCCRAC	6 cut(s)	1179, 1224, 1534, 1738, 2176, 2541
Mox20I	TGGCCA	3 cut(s)	787, 2028, 2055
MreI	CGCCGGCG	2 cut(s)	188, 194
MroI	TCCGGA	1 cut(s)	1709
MroNI	GCCGGC	2 cut(s)	188, 194
MroXI	GAANNNNTTC	1 cut(s)	711
MscI	TGGCCA	3 cut(s)	787, 2028, 2055
MseI	TTAA	9 cut(s)	858, 1058, 1488, 1562, 1667, 2219, 2223, 2376, 2549
Msp20I	TGGCCA	3 cut(s)	787, 2028, 2055
MspA1I	CMGCKG	1 cut(s)	865
MspI	CCGG	8 cut(s)	189, 195, 201, 486, 517, 1710, 1891, 2100
MspR9I	CCNGG	1 cut(s)	1544
MunI	CAATTG	2 cut(s)	1437, 1635
Mva1269I	GAATGC	2 cut(s)	713, 1823
MvaI	CCWGG	1 cut(s)	1544
MvnI	CGCG	3 cut(s)	467, 1306, 1465
MwoI	GCNNNNNNNGC	7 cut(s)	36, 204, 412, 793, 1153, 1433, 2252
NaeI	GCCGGC	2 cut(s)	190, 196
NarI	GGCGCC	2 cut(s)	192, 198
NcoI	CCATGG	3 cut(s)	1699, 2023, 2050
NgoMIV	GCCGGC	2 cut(s)	188, 194
NlaIV	GGNNCC	7 cut(s)	193, 199, 529, 1203, 1596, 1708, 2077
NmeAIII	GCCGAG	1 cut(s)	574
NmuCI	GTSAC	4 cut(s)	1093, 1406, 1913, 2033
NruI	TCGCGA	1 cut(s)	467
NspI	RCATGY	5 cut(s)	1348, 1414, 2171, 2259, 2426
PacI	TTAATTAA	1 cut(s)	2223
PaeI	GCATGC	1 cut(s)	2259
PagI	TCATGA	5 cut(s)	1371, 1678, 1777, 1813, 2014
PciI	ACATGT	2 cut(s)	1344, 1410
PctI	GAATGC	2 cut(s)	713, 1823
PdiI	GCCGGC	2 cut(s)	190, 196
PdmI	GAANNNNTTC	1 cut(s)	711
PfeI	GAWTC	7 cut(s)	475, 628, 1247, 1574, 1793, 1829, 2538
PflMI	CCANNNNNTGG	2 cut(s)	2102, 2149
PinAI	ACCGGT	1 cut(s)	2099
PleI	GAGTC	4 cut(s)	450, 497, 1246, 1745
PluTI	GGCGCC	2 cut(s)	195, 201
PpsI	GAGTC	4 cut(s)	450, 497, 1246, 1745
PscI	ACATGT	2 cut(s)	1344, 1410
PshAI	GACNNNNGTC	1 cut(s)	1911
PshBI	ATTAAT	1 cut(s)	1562
Psp124BI	GAGCTC	1 cut(s)	638
Psp6I	CCWGG	1 cut(s)	1542
PspFI	CCCAGC	4 cut(s)	447, 693, 865, 1364
PspGI	CCWGG	1 cut(s)	1542
PspN4I	GGNNCC	7 cut(s)	193, 199, 529, 1203, 1596, 1708, 2077
PspPI	GGNCC	1 cut(s)	1594
PstI	CTGCAG	1 cut(s)	2320
PsuI	RGATCY	3 cut(s)	1201, 1706, 2495
PvuII	CAGCTG	1 cut(s)	865
RruI	TCGCGA	1 cut(s)	467
RsaI	GTAC	5 cut(s)	521, 1108, 1181, 2228, 2458
RsaNI	GTAC	5 cut(s)	520, 1107, 1180, 2227, 2457
SacI	GAGCTC	1 cut(s)	638
SaqAI	TTAA	9 cut(s)	858, 1058, 1488, 1562, 1667, 2219, 2223, 2376, 2549
Sau96I	GGNCC	1 cut(s)	1594
SchI	GAGTC	4 cut(s)	450, 498, 1247, 1745
ScrFI	CCNGG	1 cut(s)	1544
SduI	GDGCHC	2 cut(s)	638, 2350
SfaNI	GCATC	4 cut(s)	1268, 1390, 1696, 2137
SfcI	CTRYAG	1 cut(s)	2316
SfoI	GGCGCC	2 cut(s)	193, 199
SgrAI	CRCCGGYG	2 cut(s)	188, 194
SinI	GGWCC	1 cut(s)	1594
SmlI	CTYRAG	2 cut(s)	1580, 1787
SmoI	CTYRAG	2 cut(s)	1580, 1787
SpeI	ACTAGT	1 cut(s)	1624
SphI	GCATGC	1 cut(s)	2259
Sse9I	AATT	7 cut(s)	844, 1139, 1355, 1437, 1635, 2220, 2337
SspDI	GGCGCC	2 cut(s)	191, 197
SspI	AATATT	1 cut(s)	2160
SspMI	CTAG	6 cut(s)	350, 545, 762, 1385, 1614, 1625
SstI	GAGCTC	1 cut(s)	638
StyD4I	CCNGG	1 cut(s)	1542
StyI	CCWWGG	4 cut(s)	639, 1699, 2023, 2050
TaaI	ACNGT	7 cut(s)	910, 1012, 1111, 1733, 1913, 2033, 2554
TaiI	ACGT	1 cut(s)	1185
TaqI	TCGA	3 cut(s)	473, 839, 2261
TasI	AATT	7 cut(s)	844, 1139, 1355, 1437, 1635, 2220, 2337
TatI	WGTACW	2 cut(s)	1106, 2226
TauI	GCSGC	6 cut(s)	140, 380, 528, 536, 572, 615
TfiI	GAWTC	7 cut(s)	475, 628, 1247, 1574, 1793, 1829, 2538
Tru1I	TTAA	9 cut(s)	858, 1058, 1488, 1562, 1667, 2219, 2223, 2376, 2549
Tru9I	TTAA	9 cut(s)	858, 1058, 1488, 1562, 1667, 2219, 2223, 2376, 2549
TscAI	CASTG	1 cut(s)	1015
TseFI	GTSAC	4 cut(s)	1093, 1406, 1913, 2033
TseI	GCWGC	4 cut(s)	793, 862, 1427, 1878
Tsp45I	GTSAC	4 cut(s)	1093, 1406, 1913, 2033
TspGWI	ACGGA	3 cut(s)	705, 789, 1252
TspRI	CASTG	1 cut(s)	1015
Van91I	CCANNNNNTGG	2 cut(s)	2102, 2149
VpaK11BI	GGWCC	1 cut(s)	1594
VspI	ATTAAT	1 cut(s)	1562
XapI	RAATTY	2 cut(s)	1355, 2337
XceI	RCATGY	5 cut(s)	1348, 1414, 2171, 2259, 2426
XcmI	CCANNNNNNNNNTGG	3 cut(s)	985, 1859, 2069
XmiI	GTMKAC	1 cut(s)	1621
XmnI	GAANNNNTTC	1 cut(s)	711
XspI	CTAG	6 cut(s)	350, 545, 762, 1385, 1614, 1625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.