Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0015244

Physical Location & Seq

Genomic Coordinates Forward (+)

4651166 .. 4653676

Gene Structure 2511 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00081570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2511 bp

ATGGCCGATCACCACCACCACCACCACCACCGCCCCCACCGCCTCTCCCTCCCTCAACGCCCCACCACCTTCGCCTCCACCACCCCCACCGCCTCCCGCCAATACCCACTCTACCCCTTCACTCCCTCCGCCGCCACTCCCAAACACCGCCTCTCTTCTCTCAACCCCAAACCCCTCTCCGCCGCCGCCACCGGAAGCAAATCCTCTCTCTCATTCCTCTTCCTCCTCCTCCTCTCCCTCCGATCACTCTACTCCCTCCTCCCCTTCCTCCGCTCCTCCCCTTCCTTCTCTCTCTTCCCCTTCTCCTTCCTCGTCTCCCTCCTCTCCTTCCTCCTAACCCTAGCTTTCTCTTTCTTCACCTCCTCCGCCGCCTCCAAAGACCCGTTTCACCAGAAGCCCAACCAGCCCATCTTCTCCCTCACTCTCATCACTCAATCCCAGCACAGACTCCTCGTCGCGAAATCGATTCTTCTCGCCGGAGTCTTCCTCCTCCGCTTCCAAGCTCTCCGGTACTGCGGCACCGCCGCCATGATCCTAGCCGAGATGTCTGGAAATGTGGCGGCAAGGTTCCTGGCGGAGGGGAGGAAACAGAGTGTGGTGGGCGGTCGTGGTGATCGGAATCGGAGCTCCAAGGTTCGTGGGTTTCTTGCTCTGTTTACTGGGTTGTTCTTGTTATCTATGAGCTGGGATCGGATTGAATGCTTTCCCTTTTCGGCTAAGTTTGTGCAGAAAATGGGAGTGTCTGTGTTGCCTAGAGTGGATTGTGTGAGAATTTGGCCAATGCTGCTTCCGTTTCTGTCTGGATTTTTGGGGTGTTATGAGAGGGTTTCGATGAATTGGGGGACTATGAGGCAGTTGGGGAGGAAGAGGGTTCGGGTGATTTCGTTGTTTTTTACTACTGTTGTGCTGTTCATTCCTGCTGTTGTTAGTGTTTTCATGTTTGAAGCTGAGGGAGATGGAGTTGTTTCCATTGGGAACTTGGGCTGGCCTCTGGCAAACACTGTTGTTTTCGGTGTGCTTCTGAGTGAGAGTTATAGCGATGAGAAGTTAATGAGTTCTAAAGATTTTCGGAGGGAGTATTTGGTGACTTTCTTATGTACTGTTGTATTGGAGCTGTTCTATTTTGCTGAATTGTCGCTTTGGGGGCTATTGCTTTGTGGTATGTTGCTGTACGTTGCTGTTAGGGAGTTGGATCCTTTTCACGCGAGTTATGTTGAACTGGGAATGGAGTCGTCGGAATCATTTAGGGCATCCGTTATGAAGCCTATTAGGCACATTTTGAGTGAGAGGAAGTCCCGAAAGATTGCACTTTTCCTTCTGATCAATACTGCATACATGTTTGTGGAATTCATTGCTGGGTTCATGAGCAATAGTCTAGGGCTCATATCAGATGCCTGTCACATGTTGTTTGATTGTGCTGCTCTGGCAATTGGGCTATATGCTTCGTATATTTCGCACTTGCCTGCAAATAATCAGTTTAACTATGGCCGTGGGAGATTTGAGGTTCTTGCAGGATATACAAATGCTGTTTTCCTGGTTTTGGTTGGAGCATTAATAGTATTGGAATCACTTGAGAGGATTTTGGACCCTCAGGAGATGTCAACTAGCAGTATACTAGTTGTTTCAATTGGAGGGCTTCTTGTCAATGTGGTTGGTTTAATCTTCTTTCATGAGGAGCATCATCATGCCCATGGTGGATCCGGATCATGCTCTCACTCCCACAGTCATCAACATTCGCATGACTCTGTTGGACATAATCATGCAGGTCATGAAAAGCTTGAGGAATCGTGTATAGCTATTTCTCATGAATGCCATGAAGAATTGTGTGCCAGCCATGATGACCATCACCAACATGGCAGTTCTGTTCGCTGCAAAGATCACCGGCACGATCACCATGATCACAGCCACCAGCATGACCAGCATGACCACAGTCACCAGCATGAGGACCATGACCACAGCCACCAGCATGACGACCATGACCACAGCCACCAGCGTGACCACCATGATCACAGCCACCAGCATGACCACCATGATCACAGCCACCAGCATGACCACCATGGCCACTGTCACCAGCATGACAACCATGGCCACAACCACCAGCATGATCACCATGGCCACAACCACCAGCATGGTGGTTCCGAGCTGAAAAGACTGCCAACCGGTGGAGAAAAATCAACTAAGCAACAGCATCGCCACATTGACCACAACATGGAAGGAATATTTCTGCATGTTTTGGCTGACACCTTAGGGAGTGTCGGAGTTGTTATATCAACACTCTTAATTAAGTACAAGGGATGGCTTGTTGCTGATCCTGCATGCTCGATATTTATTTCTGTCTTGATTATATCTTCAGTTATCCCATTGCTCAGGAACTCTGCAGAAGTCTTGCTCCAAAGAATTCCGAGGGCACATGAGCAGGATTTGAGAAAAGCTCTTAATGATGTTAGGAAGATAAGGGGGTTTCTGGTATTCGGAACTTGCATGTATGGAGTTTCACAAACTCAGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010043 GO:0012505 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0071578 GO:0072509 GO:0072511 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098739 GO:0099587

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

836

Amino Acids

94.09

Weight (kDa)

7.38

Isoelectric Point (pI)

39.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	436 - 568	3e-32	Cation efflux transmembrane domain
Cation_efflux	PF01545	727 - 797	1.5e-13	Cation efflux transmembrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04620 AT2G04620
fragaria_vesca	FvH4_6g49950
malus_domestica	MD17G1042100.v1.1
prunus_persica	Prupe.3G277100_v2.0.a1
pyrus_communis	pycom17g03760
rosa_chinensis	RchiOBHm_Chr2g0170121
rosa_laevigata	RLG00000021925
rosa_multiflora	Rmu_sc0013078.1_g000005
rosa_roxburghii	Rroxscaffold_2G00081570
rosa_rugosa	Rorug02G0545900.1
rosa_samantha	Rh2AG618000 Rh2BG629500 Rh2CG598900 Rh2DG641300
rosa_wichuraiana	Rw2G051170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1754
AccB1I	GGYRCC	1 cut(s)	518
AccB7I	CCANNNNNTGG	2 cut(s)	2162, 2209
AccBSI	CCGCTC	1 cut(s)	273
AccI	GTMKAC	1 cut(s)	1612
AccII	CGCG	2 cut(s)	458, 1205
AccIII	TCCGGA	1 cut(s)	1700
AclWI	GGATC	8 cut(s)	526, 696, 1187, 1200, 1692, 1705, 1711, 2303
AcoI	YGGCCR	6 cut(s)	3, 776, 1486, 2059, 2086, 2113
AcsI	RAATTY	3 cut(s)	771, 1346, 2397
AcuI	CTGAAG	1 cut(s)	2334
AfaI	GTAC	4 cut(s)	512, 1099, 1172, 2288
AfiI	CCNNNNNNNGG	5 cut(s)	577, 1540, 1942, 2162, 2209
AflIII	ACRYGT	2 cut(s)	1335, 1401
AgeI	ACCGGT	1 cut(s)	2159
AgsI	TTSAA	4 cut(s)	698, 944, 1217, 1626
AhdI	GACNNNNNGTC	1 cut(s)	452
AhlI	ACTAGT	1 cut(s)	1615
AjnI	CCWGG	2 cut(s)	570, 1533
AleI	CACNNNNGTG	1 cut(s)	1992
Alw21I	GWGCWC	1 cut(s)	629
Alw26I	GTCTC	1 cut(s)	319
AlwI	GGATC	8 cut(s)	526, 696, 1187, 1200, 1692, 1705, 1711, 2303
Aor13HI	TCCGGA	1 cut(s)	1700
AoxI	GGCC	7 cut(s)	3, 776, 986, 1486, 2059, 2086, 2113
ApeKI	GCWGC	3 cut(s)	784, 1418, 1869
ApoI	RAATTY	3 cut(s)	771, 1346, 2397
AseI	ATTAAT	1 cut(s)	1553
AsiGI	ACCGGT	1 cut(s)	2159
Asp700I	GAANNNNTTC	1 cut(s)	702
AspS9I	GGNCC	2 cut(s)	1585, 1945
AvaII	GGWCC	2 cut(s)	1585, 1945
AxyI	CCTNAGG	2 cut(s)	1590, 2245
BaeGI	GKGCMC	1 cut(s)	2410
BalI	TGGCCA	4 cut(s)	778, 2061, 2088, 2115
BamHI	GGATCC	2 cut(s)	1192, 1697
BanI	GGYRCC	1 cut(s)	518
BanII	GRGCYC	2 cut(s)	629, 1383
BbsI	GAAGAC	1 cut(s)	475
Bbv12I	GWGCWC	1 cut(s)	629
BbvCI	CCTCAGC	1 cut(s)	948
BbvI	GCAGC	3 cut(s)	771, 1405, 1856
BccI	CCATC	4 cut(s)	416, 950, 1851, 2289
BceAI	ACGGC	1 cut(s)	1473
BcgI	CGANNNNNNTGC	2 cut(s)	2171, 2205
BciT130I	CCWGG	2 cut(s)	572, 1535
BclI	TGATCA	5 cut(s)	1320, 1897, 2005, 2032, 2104
BcoDI	GTCTC	1 cut(s)	319
BcuI	ACTAGT	1 cut(s)	1615
BfaI	CTAG	6 cut(s)	341, 536, 753, 1376, 1605, 1616
BfmI	CTRYAG	1 cut(s)	2376
BfuAI	ACCTGC	1 cut(s)	1754
BglI	GCCNNNNNGGC	1 cut(s)	1270
Bme1390I	CCNGG	2 cut(s)	572, 1535
Bme18I	GGWCC	2 cut(s)	1585, 1945
BmeRI	GACNNNNNGTC	1 cut(s)	452
BmgT120I	GGNCC	2 cut(s)	1585, 1945
BmiI	GGNNCC	6 cut(s)	520, 569, 1194, 1587, 1699, 2137
BmrFI	CCNGG	2 cut(s)	572, 1535
BmrI	ACTGGG	2 cut(s)	669, 1229
BmsI	GCATC	4 cut(s)	1259, 1381, 1687, 2197
BmuI	ACTGGG	2 cut(s)	669, 1229
BoxI	GACNNNNGTC	1 cut(s)	1929
BpiI	GAAGAC	1 cut(s)	475
BplI	GAGNNNNNCTC	4 cut(s)	471, 503, 2416, 2448
Bpu10I	CCTNAGC	2 cut(s)	948, 2366
BpuEI	CTTGAG	2 cut(s)	1592, 1799
Bsa29I	ATCGAT	1 cut(s)	464
BsaBI	GATNNNNATC	3 cut(s)	618, 1702, 1842
BsaJI	CCNNGG	7 cut(s)	630, 1489, 1690, 2056, 2083, 2110, 2402
BsaWI	WCCGGW	4 cut(s)	191, 507, 1700, 2159
BsaXI	ACNNNNNCTCC	6 cut(s)	29, 59, 729, 759, 945, 975
Bsc4I	CCNNNNNNNGG	5 cut(s)	577, 1540, 1942, 2162, 2209
Bse118I	RCCGGY	2 cut(s)	1881, 2159
Bse1I	ACTGG	2 cut(s)	664, 1224
Bse21I	CCTNAGG	2 cut(s)	1590, 2245
Bse3DI	GCAATG	2 cut(s)	1350, 2360
Bse8I	GATNNNNATC	3 cut(s)	618, 1702, 1842
BseAI	TCCGGA	1 cut(s)	1700
BseBI	CCWGG	2 cut(s)	572, 1535
BseCI	ATCGAT	1 cut(s)	464
BseDI	CCNNGG	7 cut(s)	630, 1489, 1690, 2056, 2083, 2110, 2402
BseGI	GGATG	2 cut(s)	1250, 2300
BseJI	GATNNNNATC	3 cut(s)	618, 1702, 1842
BseLI	CCNNNNNNNGG	5 cut(s)	577, 1540, 1942, 2162, 2209
BseMI	GCAATG	2 cut(s)	1350, 2360
BseMII	CTCAG	4 cut(s)	939, 1013, 1604, 2380
BseNI	ACTGG	2 cut(s)	664, 1224
BseSI	GKGCMC	1 cut(s)	2410
BseXI	GCAGC	3 cut(s)	771, 1405, 1856
BseYI	CCCAGC	3 cut(s)	438, 684, 1355
BsgI	GTGCAG	1 cut(s)	746
Bsh1236I	CGCG	2 cut(s)	458, 1205
Bsh1285I	CGRYCG	1 cut(s)	607
BshFI	GGCC	7 cut(s)	5, 778, 988, 1488, 2061, 2088, 2115
BshNI	GGYRCC	1 cut(s)	518
BshTI	ACCGGT	1 cut(s)	2159
BshVI	ATCGAT	1 cut(s)	464
BsiEI	CGRYCG	1 cut(s)	607
BsiHKAI	GWGCWC	1 cut(s)	629
BsiSI	CCGG	6 cut(s)	192, 477, 508, 1701, 1882, 2160
BslFI	GGGAC	2 cut(s)	856, 1279
BslI	CCNNNNNNNGG	5 cut(s)	577, 1540, 1942, 2162, 2209
BsmAI	GTCTC	1 cut(s)	319
BsmBI	CGTCTC	1 cut(s)	319
BsmFI	GGGAC	2 cut(s)	856, 1279
BsmI	GAATGC	2 cut(s)	704, 1814
BsnI	GGCC	7 cut(s)	5, 778, 988, 1488, 2061, 2088, 2115
Bsp1286I	GDGCHC	3 cut(s)	629, 1383, 2410
Bsp13I	TCCGGA	1 cut(s)	1700
Bsp19I	CCATGG	4 cut(s)	1690, 2056, 2083, 2110
Bsp68I	TCGCGA	1 cut(s)	458
BspANI	GGCC	7 cut(s)	5, 778, 988, 1488, 2061, 2088, 2115
BspCNI	CTCAG	4 cut(s)	940, 1014, 1603, 2379
BspDI	ATCGAT	1 cut(s)	464
BspEI	TCCGGA	1 cut(s)	1700
BspFNI	CGCG	2 cut(s)	458, 1205
BspHI	TCATGA	4 cut(s)	1362, 1669, 1768, 1804
BspLI	GGNNCC	6 cut(s)	520, 569, 1194, 1587, 1699, 2137
BspMAI	CTGCAG	1 cut(s)	2380
BspMI	ACCTGC	1 cut(s)	1754
BspPI	GGATC	8 cut(s)	526, 696, 1187, 1200, 1692, 1705, 1711, 2303
BspT107I	GGYRCC	1 cut(s)	518
BsrBI	CCGCTC	1 cut(s)	273
BsrDI	GCAATG	2 cut(s)	1350, 2360
BsrFI	RCCGGY	2 cut(s)	1881, 2159
BsrI	ACTGG	2 cut(s)	664, 1224
BssAI	RCCGGY	2 cut(s)	1881, 2159
BssECI	CCNNGG	7 cut(s)	630, 1489, 1690, 2056, 2083, 2110, 2402
BssNAI	GTATAC	1 cut(s)	1613
BssT1I	CCWWGG	5 cut(s)	630, 1690, 2056, 2083, 2110
Bst1107I	GTATAC	1 cut(s)	1613
Bst2UI	CCWGG	2 cut(s)	572, 1535
Bst4CI	ACNGT	6 cut(s)	901, 1003, 1102, 1724, 1931, 2066
Bst6I	CTCTTC	4 cut(s)	160, 224, 299, 860
BstC8I	GCNNGC	4 cut(s)	986, 1464, 1831, 2317
BstDEI	CTNAG	8 cut(s)	717, 948, 1022, 1590, 2178, 2245, 2366, 2502
BstDSI	CCRYGG	5 cut(s)	1489, 1690, 2056, 2083, 2110
BstENI	CCTNNNNNAGG	1 cut(s)	575
BstF5I	GGATG	2 cut(s)	1250, 2300
BstFNI	CGCG	2 cut(s)	458, 1205
BstMAI	GTCTC	1 cut(s)	319
BstMCI	CGRYCG	1 cut(s)	607
BstMWI	GCNNNNNNNGC	8 cut(s)	39, 403, 784, 1144, 1270, 1424, 1918, 2312
BstNI	CCWGG	2 cut(s)	572, 1535
BstNSI	RCATGY	5 cut(s)	1339, 1405, 2231, 2319, 2485
BstPAI	GACNNNNGTC	1 cut(s)	1929
BstSCI	CCNGG	2 cut(s)	570, 1533
BstSFI	CTRYAG	1 cut(s)	2376
BstSLI	GKGCMC	1 cut(s)	2410
BstUI	CGCG	2 cut(s)	458, 1205
BstV1I	GCAGC	3 cut(s)	771, 1405, 1856
BstV2I	GAAGAC	1 cut(s)	475
BstX2I	RGATCY	2 cut(s)	1192, 1697
BstXI	CCANNNNNNTGG	2 cut(s)	2129, 2132
BstYI	RGATCY	2 cut(s)	1192, 1697
BstZ17I	GTATAC	1 cut(s)	1613
Bsu15I	ATCGAT	1 cut(s)	464
Bsu36I	CCTNAGG	2 cut(s)	1590, 2245
BsuRI	GGCC	7 cut(s)	5, 778, 988, 1488, 2061, 2088, 2115
BsuTUI	ATCGAT	1 cut(s)	464
BtgI	CCRYGG	5 cut(s)	1489, 1690, 2056, 2083, 2110
BtgZI	GCGATG	2 cut(s)	1053, 2174
BtsCI	GGATG	2 cut(s)	1250, 2300
BtsIMutI	CAGTG	2 cut(s)	999, 2062
BtuMI	TCGCGA	1 cut(s)	458
BveI	ACCTGC	1 cut(s)	1754
Cac8I	GCNNGC	4 cut(s)	986, 1464, 1831, 2317
CciI	TCATGA	4 cut(s)	1362, 1669, 1768, 1804
Cfr10I	RCCGGY	2 cut(s)	1881, 2159
Cfr13I	GGNCC	2 cut(s)	1585, 1945
ClaI	ATCGAT	1 cut(s)	464
Csp6I	GTAC	4 cut(s)	511, 1098, 1171, 2287
CspAI	ACCGGT	1 cut(s)	2159
CspCI	CAANNNNNGTGG	2 cut(s)	619, 654
CviQI	GTAC	4 cut(s)	511, 1098, 1171, 2287
DdeI	CTNAG	8 cut(s)	717, 948, 1022, 1590, 2178, 2245, 2366, 2502
DriI	GACNNNNNGTC	1 cut(s)	452
EaeI	YGGCCR	6 cut(s)	3, 776, 1486, 2059, 2086, 2113
Eam1104I	CTCTTC	4 cut(s)	160, 224, 299, 860
Eam1105I	GACNNNNNGTC	1 cut(s)	452
EarI	CTCTTC	4 cut(s)	160, 224, 299, 860
EciI	GGCGGA	4 cut(s)	118, 169, 355, 590
Ecl136II	GAGCTC	1 cut(s)	627
Eco130I	CCWWGG	5 cut(s)	630, 1690, 2056, 2083, 2110
Eco24I	GRGCYC	2 cut(s)	629, 1383
Eco47I	GGWCC	2 cut(s)	1585, 1945
Eco53kI	GAGCTC	1 cut(s)	627
Eco57I	CTGAAG	1 cut(s)	2334
Eco81I	CCTNAGG	2 cut(s)	1590, 2245
EcoICRI	GAGCTC	1 cut(s)	627
EcoNI	CCTNNNNNAGG	1 cut(s)	575
EcoRI	GAATTC	2 cut(s)	1346, 2397
EcoRII	CCWGG	2 cut(s)	570, 1533
EcoT14I	CCWWGG	5 cut(s)	630, 1690, 2056, 2083, 2110
EcoT38I	GRGCYC	2 cut(s)	629, 1383
ErhI	CCWWGG	5 cut(s)	630, 1690, 2056, 2083, 2110
Esp3I	CGTCTC	1 cut(s)	319
FaqI	GGGAC	2 cut(s)	856, 1279
FauI	CCCGC	1 cut(s)	104
FbaI	TGATCA	5 cut(s)	1320, 1897, 2005, 2032, 2104
FblI	GTMKAC	1 cut(s)	1612
FokI	GGATG	2 cut(s)	1237, 2307
FriOI	GRGCYC	2 cut(s)	629, 1383
FspBI	CTAG	6 cut(s)	341, 536, 753, 1376, 1605, 1616
GsaI	CCCAGC	3 cut(s)	442, 688, 1359
HaeIII	GGCC	7 cut(s)	5, 778, 988, 1488, 2061, 2088, 2115
HapII	CCGG	6 cut(s)	192, 477, 508, 1701, 1882, 2160
HincII	GTYRAC	1 cut(s)	1602
HindII	GTYRAC	1 cut(s)	1602
HindIII	AAGCTT	1 cut(s)	1775
HinfI	GANTC	9 cut(s)	447, 466, 480, 619, 1229, 1238, 1565, 1742, 1784
HpaII	CCGG	6 cut(s)	192, 477, 508, 1701, 1882, 2160
Hpy166II	GTNNAC	3 cut(s)	657, 1602, 1613
Hpy8I	GTNNAC	3 cut(s)	657, 1602, 1613
Hpy99I	CGWCG	2 cut(s)	458, 1237
HpyCH4III	ACNGT	6 cut(s)	901, 1003, 1102, 1724, 1931, 2066
HpyCH4IV	ACGT	1 cut(s)	1173
HpyF10VI	GCNNNNNNNGC	8 cut(s)	39, 403, 784, 1144, 1270, 1424, 1918, 2312
HpyF3I	CTNAG	8 cut(s)	717, 948, 1022, 1590, 2178, 2245, 2366, 2502
HpySE526I	ACGT	1 cut(s)	1173
Kpn2I	TCCGGA	1 cut(s)	1700
Ksp22I	TGATCA	5 cut(s)	1320, 1897, 2005, 2032, 2104
LmnI	GCTCC	7 cut(s)	278, 624, 632, 1111, 1547, 1675, 2394
Lsp1109I	GCAGC	3 cut(s)	771, 1405, 1856
LweI	GCATC	4 cut(s)	1259, 1381, 1687, 2197
MaeI	CTAG	6 cut(s)	341, 536, 753, 1376, 1605, 1616
MaeII	ACGT	1 cut(s)	1173
MaeIII	GTNAC	5 cut(s)	1084, 1397, 1931, 1994, 2066
MbiI	CCGCTC	1 cut(s)	273
MfeI	CAATTG	2 cut(s)	1428, 1626
MflI	RGATCY	2 cut(s)	1192, 1697
MhlI	GDGCHC	3 cut(s)	629, 1383, 2410
MlsI	TGGCCA	4 cut(s)	778, 2061, 2088, 2115
MluCI	AATT	9 cut(s)	771, 835, 1130, 1346, 1428, 1626, 1820, 2280, 2397
MluNI	TGGCCA	4 cut(s)	778, 2061, 2088, 2115
MlyI	GAGTC	4 cut(s)	441, 489, 1238, 1736
MmeI	TCCRAC	5 cut(s)	1170, 1215, 1525, 1729, 2236
Mox20I	TGGCCA	4 cut(s)	778, 2061, 2088, 2115
MroI	TCCGGA	1 cut(s)	1700
MroXI	GAANNNNTTC	1 cut(s)	702
MscI	TGGCCA	4 cut(s)	778, 2061, 2088, 2115
MseI	TTAA	7 cut(s)	1049, 1479, 1553, 1658, 2279, 2283, 2436
Msp20I	TGGCCA	4 cut(s)	778, 2061, 2088, 2115
MspI	CCGG	6 cut(s)	192, 477, 508, 1701, 1882, 2160
MspR9I	CCNGG	2 cut(s)	572, 1535
MunI	CAATTG	2 cut(s)	1428, 1626
Mva1269I	GAATGC	2 cut(s)	704, 1814
MvaI	CCWGG	2 cut(s)	572, 1535
MvnI	CGCG	2 cut(s)	458, 1205
MwoI	GCNNNNNNNGC	8 cut(s)	39, 403, 784, 1144, 1270, 1424, 1918, 2312
NcoI	CCATGG	4 cut(s)	1690, 2056, 2083, 2110
NlaIV	GGNNCC	6 cut(s)	520, 569, 1194, 1587, 1699, 2137
NmeAIII	GCCGAG	1 cut(s)	565
NmuCI	GTSAC	5 cut(s)	1084, 1397, 1931, 1994, 2066
NruI	TCGCGA	1 cut(s)	458
NspI	RCATGY	5 cut(s)	1339, 1405, 2231, 2319, 2485
OliI	CACNNNNGTG	1 cut(s)	1992
PacI	TTAATTAA	1 cut(s)	2283
PaeI	GCATGC	1 cut(s)	2319
PagI	TCATGA	4 cut(s)	1362, 1669, 1768, 1804
PciI	ACATGT	2 cut(s)	1335, 1401
PctI	GAATGC	2 cut(s)	704, 1814
PdmI	GAANNNNTTC	1 cut(s)	702
PfeI	GAWTC	5 cut(s)	466, 619, 1238, 1565, 1784
PflMI	CCANNNNNTGG	2 cut(s)	2162, 2209
PinAI	ACCGGT	1 cut(s)	2159
PleI	GAGTC	4 cut(s)	441, 488, 1237, 1736
PpsI	GAGTC	4 cut(s)	441, 488, 1237, 1736
PscI	ACATGT	2 cut(s)	1335, 1401
PshAI	GACNNNNGTC	1 cut(s)	1929
PshBI	ATTAAT	1 cut(s)	1553
Psp124BI	GAGCTC	1 cut(s)	629
Psp6I	CCWGG	2 cut(s)	570, 1533
PspFI	CCCAGC	3 cut(s)	438, 684, 1355
PspGI	CCWGG	2 cut(s)	570, 1533
PspN4I	GGNNCC	6 cut(s)	520, 569, 1194, 1587, 1699, 2137
PspPI	GGNCC	2 cut(s)	1585, 1945
PstI	CTGCAG	1 cut(s)	2380
PsuI	RGATCY	2 cut(s)	1192, 1697
RruI	TCGCGA	1 cut(s)	458
RsaI	GTAC	4 cut(s)	512, 1099, 1172, 2288
RsaNI	GTAC	4 cut(s)	511, 1098, 1171, 2287
SacI	GAGCTC	1 cut(s)	629
SaqAI	TTAA	7 cut(s)	1049, 1479, 1553, 1658, 2279, 2283, 2436
Sau96I	GGNCC	2 cut(s)	1585, 1945
SchI	GAGTC	4 cut(s)	441, 489, 1238, 1736
ScrFI	CCNGG	2 cut(s)	572, 1535
SduI	GDGCHC	3 cut(s)	629, 1383, 2410
SfaNI	GCATC	4 cut(s)	1259, 1381, 1687, 2197
SfcI	CTRYAG	1 cut(s)	2376
SinI	GGWCC	2 cut(s)	1585, 1945
SmlI	CTYRAG	2 cut(s)	1571, 1778
SmoI	CTYRAG	2 cut(s)	1571, 1778
SpeI	ACTAGT	1 cut(s)	1615
SphI	GCATGC	1 cut(s)	2319
Sse9I	AATT	9 cut(s)	771, 835, 1130, 1346, 1428, 1626, 1820, 2280, 2397
SspI	AATATT	1 cut(s)	2220
SspMI	CTAG	6 cut(s)	341, 536, 753, 1376, 1605, 1616
SstI	GAGCTC	1 cut(s)	629
StyD4I	CCNGG	2 cut(s)	570, 1533
StyI	CCWWGG	5 cut(s)	630, 1690, 2056, 2083, 2110
TaaI	ACNGT	6 cut(s)	901, 1003, 1102, 1724, 1931, 2066
TaiI	ACGT	1 cut(s)	1176
TaqI	TCGA	3 cut(s)	464, 830, 2321
TasI	AATT	9 cut(s)	771, 835, 1130, 1346, 1428, 1626, 1820, 2280, 2397
TatI	WGTACW	2 cut(s)	1097, 2286
TauI	GCSGC	7 cut(s)	134, 185, 188, 371, 519, 527, 563
TfiI	GAWTC	5 cut(s)	466, 619, 1238, 1565, 1784
Tru1I	TTAA	7 cut(s)	1049, 1479, 1553, 1658, 2279, 2283, 2436
Tru9I	TTAA	7 cut(s)	1049, 1479, 1553, 1658, 2279, 2283, 2436
TscAI	CASTG	2 cut(s)	1006, 2069
TseFI	GTSAC	5 cut(s)	1084, 1397, 1931, 1994, 2066
TseI	GCWGC	3 cut(s)	784, 1418, 1869
Tsp45I	GTSAC	5 cut(s)	1084, 1397, 1931, 1994, 2066
TspGWI	ACGGA	2 cut(s)	780, 1243
TspRI	CASTG	2 cut(s)	1006, 2069
Van91I	CCANNNNNTGG	2 cut(s)	2162, 2209
VpaK11BI	GGWCC	2 cut(s)	1585, 1945
VspI	ATTAAT	1 cut(s)	1553
XagI	CCTNNNNNAGG	1 cut(s)	575
XapI	RAATTY	3 cut(s)	771, 1346, 2397
XceI	RCATGY	5 cut(s)	1339, 1405, 2231, 2319, 2485
XcmI	CCANNNNNNNNNTGG	3 cut(s)	976, 1850, 2129
XmiI	GTMKAC	1 cut(s)	1612
XmnI	GAANNNNTTC	1 cut(s)	702
XspI	CTAG	6 cut(s)	341, 536, 753, 1376, 1605, 1616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.