MD17G1085500.v1.1

Protein THYLAKOID FORMATION1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011451
Physical Location & Seq
Forward (+)
7023536 .. 7026493
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1085500.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGCGGCTGTTGCTGCTCTGTCTTTCTCGGCACTCAGTCCATGCTCTGATAGAAAATCGGTGGTCTCGTCCACTCGGAATTTGGCTTCCACCGCCGATGGCCTTCGATTCCGTAGGTGCATTTCCAGCCGTAGCGGTGGAATTCGAGCTTCGAGTTCGAGCTCTCGCATGGTGGTTCATTGCATGGCAGGATCGTCAGATCCTCCGACTGTGGCGGATACAAAGTTGAAATTTTTGAAGGCGTATAAGCGACCAATCCCTAGCGTTTACAACAGTGTCCTGCAGGAGCTGATTGTGCAGCAACATTTGATGAAGTACAAGAGGACATACCGGTATGATCCTGTGTTCGCTCTTGGTTTTGTCACTGTATACGATCAGCTCATGGATGGATACCCCAGCGATGATGATCGAGAGGCAATCTTCCAAGCGTACATTAAAGCGTTAAATGAGGACCCAGAGCAATACAGAACTGATGCACAAAAGTTGGAAGAGTGGGCTAGGGCGCAAACTTCCAGTTCATTAGTGGAGTTCTCATCCAGAGAAGGAGAAGTTGAGGCAGCACTGAAGGACATTGCAGAGAGGGCAGCAGGAAAAGGAAGTTTTAGCTACAGTCGTTTCTTTGCTATTGGGCTTTTTCGTCTTCTTGAGGTGGCAAAAGCAACTGAACCAACAGTATTAGAAAAGTTATGTGGAGCCTTAAACATTGACAAAAGAAGTGTGGACCGGGACCTTGATGTGTACCGCAATCTGCTTTCCAAGCTGGTTCAGGCCAAAGAACTGCTAAGGGAATATGTGGACAGGGAGAAGAAGAAAAGAGAAGAACGGGCGGAAACACAGAGGGCCAGTGAAACAGTTACGAAATGTTTGGGGGACTATGACTATGTTAGCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006605 GO:0006612 GO:0006725 GO:0006778 GO:0006787 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009507 GO:0009526 GO:0009527 GO:0009528 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009668 GO:0009707 GO:0009743 GO:0009756 GO:0009889 GO:0009941 GO:0009987 GO:0010027 GO:0010033 GO:0010119 GO:0010182 GO:0010207 GO:0010319 GO:0015031 GO:0015833 GO:0015979 GO:0015994 GO:0015996 GO:0016020 GO:0016043 GO:0017038 GO:0019222 GO:0019439 GO:0019684 GO:0019866 GO:0019867 GO:0022607 GO:0023052 GO:0031090 GO:0031323 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0031976 GO:0031984 GO:0033013 GO:0033015 GO:0033036 GO:0033365 GO:0034357 GO:0034613 GO:0034622 GO:0034641 GO:0042170 GO:0042221 GO:0042440 GO:0042651 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044248 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045036 GO:0045037 GO:0045038 GO:0045184 GO:0046149 GO:0046483 GO:0046700 GO:0046907 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051187 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055035 GO:0055085 GO:0061024 GO:0065002 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071322 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0072657 GO:0080134 GO:0090150 GO:0098588 GO:0098805 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1901701 GO:1902456 GO:1902458 GO:1903426 GO:2000070 GO:2000377
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThylakoidFormat

Protein Analysis

298

Amino Acids

33.42

Weight (kDa)

8.81

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThylakoidFormat PF11264 70 - 275 1.5e-76 Thylakoid formation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 369
AciI CCGC 6 cut(s) 5, 93, 135, 215, 742, 827
AclWI GGATC 3 cut(s) 194, 199, 332
AcsI RAATTY 3 cut(s) 79, 141, 230
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 3 cut(s) 317, 431, 740
AgeI ACCGGT 1 cut(s) 330
AgsI TTSAA 2 cut(s) 229, 238
AluBI AGCT 6 cut(s) 149, 162, 289, 379, 606, 760
AluI AGCT 6 cut(s) 149, 162, 289, 379, 606, 760
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 3 cut(s) 194, 199, 332
AlwNI CAGNNNCTG 1 cut(s) 289
AoxI GGCC 3 cut(s) 100, 768, 840
ApeKI GCWGC 4 cut(s) 14, 298, 557, 584
ApoI RAATTY 3 cut(s) 79, 141, 230
AsiGI ACCGGT 1 cut(s) 330
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 4 cut(s) 451, 721, 727, 840
AsuC2I CCSGG 1 cut(s) 725
AvaII GGWCC 3 cut(s) 451, 721, 727
BanII GRGCYC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 3 cut(s) 310, 569, 596
BccI CCATC 2 cut(s) 92, 380
BceAI ACGGC 1 cut(s) 114
BciVI GTATCC 2 cut(s) 211, 383
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 2 cut(s) 261, 498
BfmI CTRYAG 2 cut(s) 281, 607
BfuI GTATCC 2 cut(s) 211, 383
BisI GCNGC 5 cut(s) 6, 15, 299, 558, 585
BlsI GCNGC 5 cut(s) 7, 16, 300, 559, 586
Bme1390I CCNGG 1 cut(s) 725
Bme18I GGWCC 3 cut(s) 451, 721, 727
BmgT120I GGNCC 4 cut(s) 451, 721, 727, 840
BmiI GGNNCC 3 cut(s) 453, 694, 728
BmrFI CCNGG 1 cut(s) 725
BmsI GCATC 1 cut(s) 463
BpiI GAAGAC 1 cut(s) 632
Bpu10I CCTNAGC 1 cut(s) 782
BpuEI CTTGAG 1 cut(s) 665
BpuMI CCSGG 1 cut(s) 725
BsaBI GATNNNNATC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 70
BsaWI WCCGGW 1 cut(s) 330
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 3 cut(s) 513, 843, 889
Bse3DI GCAATG 2 cut(s) 178, 570
Bse8I GATNNNNATC 1 cut(s) 405
BseGI GGATG 2 cut(s) 391, 533
BseJI GATNNNNATC 1 cut(s) 405
BseMI GCAATG 2 cut(s) 178, 570
BseMII CTCAG 1 cut(s) 49
BseNI ACTGG 3 cut(s) 513, 843, 889
BseXI GCAGC 3 cut(s) 310, 569, 596
BseYI CCCAGC 1 cut(s) 395
BsgI GTGCAG 1 cut(s) 317
BshFI GGCC 3 cut(s) 102, 770, 842
BshTI ACCGGT 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 2 cut(s) 331, 724
BslFI GGGAC 2 cut(s) 740, 884
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 2 cut(s) 740, 884
BsnI GGCC 3 cut(s) 102, 770, 842
Bso31I GGTCTC 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 5 cut(s) 191, 199, 337, 373, 406
BspACI CCGC 6 cut(s) 5, 93, 135, 215, 742, 827
BspANI GGCC 3 cut(s) 102, 770, 842
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 453, 694, 728
BspMAI CTGCAG 1 cut(s) 285
BspPI GGATC 3 cut(s) 194, 199, 332
BspTNI GGTCTC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 178, 570
BsrFI RCCGGY 1 cut(s) 330
BsrI ACTGG 3 cut(s) 513, 843, 889
BssAI RCCGGY 1 cut(s) 330
BssMI GATC 5 cut(s) 191, 199, 337, 373, 406
BssNAI GTATAC 1 cut(s) 370
Bst1107I GTATAC 1 cut(s) 370
Bst4CI ACNGT 6 cut(s) 211, 275, 367, 611, 673, 853
Bst6I CTCTTC 1 cut(s) 483
BstDEI CTNAG 2 cut(s) 35, 782
BstF5I GGATG 2 cut(s) 391, 533
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 5 cut(s) 194, 202, 340, 376, 409
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 5 cut(s) 191, 199, 337, 373, 406
BstMWI GCNNNNNNNGC 7 cut(s) 11, 14, 92, 126, 295, 581, 757
BstSCI CCNGG 1 cut(s) 723
BstSFI CTRYAG 2 cut(s) 281, 607
BstV1I GCAGC 3 cut(s) 310, 569, 596
BstV2I GAAGAC 1 cut(s) 632
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
BstZ17I GTATAC 1 cut(s) 370
BsuI GTATCC 2 cut(s) 211, 383
BsuRI GGCC 3 cut(s) 102, 770, 842
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 2 cut(s) 391, 533
BtsIMutI CAGTG 4 cut(s) 280, 363, 560, 850
CaiI CAGNNNCTG 1 cut(s) 289
CfoI GCGC 1 cut(s) 505
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 4 cut(s) 451, 721, 727, 840
Csp6I GTAC 3 cut(s) 316, 430, 739
CspAI ACCGGT 1 cut(s) 330
CviAII CATG 4 cut(s) 42, 169, 184, 382
CviQI GTAC 3 cut(s) 316, 430, 739
DdeI CTNAG 2 cut(s) 35, 782
DpnI GATC 5 cut(s) 193, 201, 339, 375, 408
DpnII GATC 5 cut(s) 191, 199, 337, 373, 406
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EciI GGCGGA 2 cut(s) 230, 842
Ecl136II GAGCTC 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 1 cut(s) 70
Eco47I GGWCC 3 cut(s) 451, 721, 727
Eco53kI GAGCTC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 584
EcoICRI GAGCTC 1 cut(s) 162
EcoO109I RGGNCCY 2 cut(s) 451, 727
EcoRI GAATTC 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 164
FaeI CATG 4 cut(s) 45, 172, 187, 385
FaqI GGGAC 2 cut(s) 740, 884
FatI CATG 4 cut(s) 41, 168, 183, 381
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 5 cut(s) 6, 15, 299, 558, 585
FokI GGATG 2 cut(s) 398, 520
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 5 cut(s) 6, 15, 299, 558, 585
FspBI CTAG 2 cut(s) 261, 498
GlaI GCGC 1 cut(s) 504
GluI GCNGC 5 cut(s) 6, 15, 299, 558, 585
GsaI CCCAGC 1 cut(s) 399
HaeIII GGCC 3 cut(s) 102, 770, 842
HapII CCGG 2 cut(s) 331, 724
HhaI GCGC 1 cut(s) 505
Hin1II CATG 4 cut(s) 45, 172, 187, 385
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HinfI GANTC 1 cut(s) 108
HpaII CCGG 2 cut(s) 331, 724
Hpy166II GTNNAC 6 cut(s) 72, 268, 370, 721, 739, 796
Hpy188I TCNGA 4 cut(s) 49, 78, 199, 207
Hpy188III TCNNGA 3 cut(s) 410, 537, 644
Hpy8I GTNNAC 6 cut(s) 72, 268, 370, 721, 739, 796
HpyAV CCTTC 4 cut(s) 113, 232, 536, 559
HpyCH4III ACNGT 6 cut(s) 211, 275, 367, 611, 673, 853
HpyCH4V TGCA 6 cut(s) 120, 183, 283, 298, 476, 575
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 14, 92, 126, 295, 581, 757
HpyF3I CTNAG 2 cut(s) 35, 782
Hsp92II CATG 4 cut(s) 45, 172, 187, 385
HspAI GCGC 1 cut(s) 503
Kzo9I GATC 5 cut(s) 191, 199, 337, 373, 406
LmnI GCTCC 2 cut(s) 286, 692
Lsp1109I GCAGC 3 cut(s) 310, 569, 596
LweI GCATC 1 cut(s) 463
MaeI CTAG 2 cut(s) 261, 498
MaeIII GTNAC 2 cut(s) 361, 853
MalI GATC 5 cut(s) 193, 201, 339, 375, 408
MboI GATC 5 cut(s) 191, 199, 337, 373, 406
MboII GAAGA 6 cut(s) 412, 500, 632, 817, 820, 830
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 3 cut(s) 79, 141, 230
MmeI TCCRAC 2 cut(s) 230, 465
MnlI CCTC 8 cut(s) 213, 315, 406, 442, 547, 573, 640, 831
MseI TTAA 3 cut(s) 435, 443, 698
MspI CCGG 2 cut(s) 331, 724
MspR9I CCNGG 1 cut(s) 725
MwoI GCNNNNNNNGC 7 cut(s) 11, 14, 92, 126, 295, 581, 757
NciI CCSGG 1 cut(s) 725
NdeII GATC 5 cut(s) 191, 199, 337, 373, 406
NlaIII CATG 4 cut(s) 45, 172, 187, 385
NlaIV GGNNCC 3 cut(s) 453, 694, 728
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PfeI GAWTC 1 cut(s) 108
PinAI ACCGGT 1 cut(s) 330
PkrI GCNGC 5 cut(s) 7, 16, 300, 559, 586
PpuMI RGGWCCY 2 cut(s) 451, 727
Psp124BI GAGCTC 1 cut(s) 164
Psp5II RGGWCCY 2 cut(s) 451, 727
PspFI CCCAGC 1 cut(s) 395
PspN4I GGNNCC 3 cut(s) 453, 694, 728
PspPI GGNCC 4 cut(s) 451, 721, 727, 840
PspPPI RGGWCCY 2 cut(s) 451, 727
PstI CTGCAG 1 cut(s) 285
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 3 cut(s) 317, 431, 740
RsaNI GTAC 3 cut(s) 316, 430, 739
SacI GAGCTC 1 cut(s) 164
SaqAI TTAA 3 cut(s) 435, 443, 698
SatI GCNGC 5 cut(s) 6, 15, 299, 558, 585
Sau3AI GATC 5 cut(s) 191, 199, 337, 373, 406
Sau96I GGNCC 4 cut(s) 451, 721, 727, 840
SbfI CCTGCAGG 1 cut(s) 285
ScrFI CCNGG 1 cut(s) 725
SdaI CCTGCAGG 1 cut(s) 285
SduI GDGCHC 1 cut(s) 164
SetI ASST 9 cut(s) 119, 151, 164, 291, 381, 608, 651, 732, 762
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 2 cut(s) 281, 607
SinI GGWCC 3 cut(s) 451, 721, 727
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse8387I CCTGCAGG 1 cut(s) 285
Sse9I AATT 3 cut(s) 79, 141, 230
SsiI CCGC 6 cut(s) 5, 93, 135, 215, 742, 827
SspMI CTAG 2 cut(s) 261, 498
SstI GAGCTC 1 cut(s) 164
StyD4I CCNGG 1 cut(s) 723
TaaI ACNGT 6 cut(s) 211, 275, 367, 611, 673, 853
TaqI TCGA 5 cut(s) 106, 145, 152, 158, 409
TasI AATT 3 cut(s) 79, 141, 230
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 3 cut(s) 435, 443, 698
Tru9I TTAA 3 cut(s) 435, 443, 698
TscAI CASTG 4 cut(s) 280, 370, 567, 850
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 4 cut(s) 14, 298, 557, 584
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 3 cut(s) 167, 326, 507
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 4 cut(s) 280, 370, 567, 850
VpaK11BI GGWCC 3 cut(s) 451, 721, 727
XapI RAATTY 3 cut(s) 79, 141, 230
XcmI CCANNNNNNNNNTGG 2 cut(s) 79, 520
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 2 cut(s) 261, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.