Rmu_sc0003548.1_g000004

Protein THYLAKOID FORMATION1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003548.1
OG0011451
Physical Location & Seq
Reverse (-)
19976 .. 22642
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003548.1_g000004.1.cds

Sequence Viewer

Length: 879 bp
atggccgccgtcacttctctctctttctctgctctaactcagtcgtctttcccgtcggctagaaatttggtttcgaactccgatggtctgcgattccgtaccagcttctcgctgcattatggtggcttccggtcatccaattccagccctcgtttggttgttcattgcatgtcttcgacttcagatttggcaactgtagctgatacgaagttgaattttctaaaggcgtataagcggccgatcccgagtgtgtacaacactgtgttgcaggagctgattgtgcagcaacatttgatgaggtacaagaggacctaccgttacgaccctgtgtttgctcttggctttgttactgtatatgatcagctcatggatgggtaccctagcgatgaggatagagatgcaatcttcagagcttacataaatgcactaaaggaggacccggagcaatacagagctgatgcaaaaaagttggaagagtgggctcgggctcagacttccagttcgttgattgaatttccgtccaaagaaggagaagttgaggggatattgaaggacattgcagaaagagcaggtggcaaaggaagttttagctatagccgcttctttgcggttgggctctttcgtcttcttgagttggcaaatgcaactgaaccaacagtattggaaaagttgtgtgcagccttaaacattgagaaaagaagtgtggaccgggaccttgatgtataccgcaatctactttctaagctggttcaggcaaaagaactgctaaaggaatatgtggccagggagaagaagaaaagggaagaaagggcaggagcacagaaggctaatgaagccattgcaacgtgtttgggggagccccaatatatgggcctgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006605 GO:0006612 GO:0006725 GO:0006778 GO:0006787 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009507 GO:0009526 GO:0009527 GO:0009528 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009668 GO:0009707 GO:0009743 GO:0009756 GO:0009889 GO:0009941 GO:0009987 GO:0010027 GO:0010033 GO:0010119 GO:0010182 GO:0010207 GO:0010319 GO:0015031 GO:0015833 GO:0015979 GO:0015994 GO:0015996 GO:0016020 GO:0016043 GO:0017038 GO:0019222 GO:0019439 GO:0019684 GO:0019866 GO:0019867 GO:0022607 GO:0023052 GO:0031090 GO:0031323 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0031976 GO:0031984 GO:0033013 GO:0033015 GO:0033036 GO:0033365 GO:0034357 GO:0034613 GO:0034622 GO:0034641 GO:0042170 GO:0042221 GO:0042440 GO:0042651 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044248 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045036 GO:0045037 GO:0045038 GO:0045184 GO:0046149 GO:0046483 GO:0046700 GO:0046907 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051187 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055035 GO:0055085 GO:0061024 GO:0065002 GO:0065003 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071322 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0072657 GO:0080134 GO:0090150 GO:0098588 GO:0098805 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1901701 GO:1902456 GO:1902458 GO:1903426 GO:2000070 GO:2000377
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ThylakoidFormat

Protein Analysis

292

Amino Acids

32.91

Weight (kDa)

8.78

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 560
Acc36I ACCTGC 1 cut(s) 560
Acc65I GGTACC 1 cut(s) 375
AccB1I GGYRCC 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 868
AccI GTMKAC 1 cut(s) 723
AciI CCGC 5 cut(s) 6, 235, 598, 608, 727
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 3 cut(s) 3, 236, 780
AcsI RAATTY 3 cut(s) 64, 214, 512
AcuI CTGAAG 2 cut(s) 165, 391
AfaI GTAC 4 cut(s) 100, 254, 302, 377
AfiI CCNNNNNNNGG 2 cut(s) 154, 868
AflIII ACRYGT 1 cut(s) 845
AgsI TTSAA 3 cut(s) 214, 512, 550
AjnI CCWGG 1 cut(s) 782
AluBI AGCT 8 cut(s) 105, 200, 274, 364, 413, 455, 591, 745
AluI AGCT 8 cut(s) 105, 200, 274, 364, 413, 455, 591, 745
Alw21I GWGCWC 1 cut(s) 820
AlwI GGATC 1 cut(s) 235
AlwNI CAGNNNCTG 1 cut(s) 274
Ama87I CYCGRG 2 cut(s) 244, 483
AoxI GGCC 4 cut(s) 3, 236, 780, 871
ApeKI GCWGC 3 cut(s) 112, 283, 677
ApoI RAATTY 3 cut(s) 64, 214, 512
ArsI GACNNNNNNTTYG 6 cut(s) 169, 201, 314, 346, 484, 516
Asp718I GGTACC 1 cut(s) 375
AspS9I GGNCC 5 cut(s) 309, 436, 706, 712, 871
AsuC2I CCSGG 2 cut(s) 440, 710
AsuII TTCGAA 1 cut(s) 74
AvaI CYCGRG 2 cut(s) 244, 483
AvaII GGWCC 4 cut(s) 309, 436, 706, 712
BalI TGGCCA 1 cut(s) 782
BanI GGYRCC 1 cut(s) 375
BanII GRGCYC 4 cut(s) 484, 490, 618, 861
BbsI GAAGAC 2 cut(s) 165, 617
Bbv12I GWGCWC 1 cut(s) 820
BbvI GCAGC 3 cut(s) 99, 295, 689
BccI CCATC 2 cut(s) 77, 365
BciT130I CCWGG 1 cut(s) 784
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 2 cut(s) 440, 710
BfaI CTAG 2 cut(s) 60, 381
BfmI CTRYAG 3 cut(s) 195, 592, 875
BfuAI ACCTGC 1 cut(s) 560
BisI GCNGC 6 cut(s) 6, 113, 236, 284, 598, 678
BlsI GCNGC 6 cut(s) 7, 114, 237, 285, 599, 679
Bme1390I CCNGG 3 cut(s) 440, 710, 784
Bme18I GGWCC 4 cut(s) 309, 436, 706, 712
BmeT110I CYCGRG 2 cut(s) 244, 483
BmgT120I GGNCC 5 cut(s) 309, 436, 706, 712, 871
BmiI GGNNCC 4 cut(s) 377, 438, 713, 858
BmrFI CCNGG 3 cut(s) 440, 710, 784
BmsI GCATC 2 cut(s) 388, 448
BpiI GAAGAC 2 cut(s) 165, 617
Bpu14I TTCGAA 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 2 cut(s) 440, 710
BsaJI CCNNGG 1 cut(s) 783
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 868
Bse1I ACTGG 1 cut(s) 498
Bse3DI GCAATG 3 cut(s) 163, 555, 837
BseBI CCWGG 1 cut(s) 784
BseDI CCNNGG 1 cut(s) 783
BseGI GGATG 2 cut(s) 134, 376
BseLI CCNNNNNNNGG 2 cut(s) 154, 868
BseMI GCAATG 3 cut(s) 163, 555, 837
BseMII CTCAG 2 cut(s) 53, 503
BseNI ACTGG 1 cut(s) 498
BseX3I CGGCCG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 99, 295, 689
BsgI GTGCAG 2 cut(s) 302, 696
Bsh1285I CGRYCG 1 cut(s) 239
BshFI GGCC 4 cut(s) 5, 238, 782, 873
BshNI GGYRCC 1 cut(s) 375
BsiEI CGRYCG 1 cut(s) 239
BsiHKAI GWGCWC 1 cut(s) 820
BsiHKCI CYCGRG 2 cut(s) 244, 483
BsiSI CCGG 3 cut(s) 130, 440, 709
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 2 cut(s) 154, 868
BsmFI GGGAC 1 cut(s) 725
BsnI GGCC 4 cut(s) 5, 238, 782, 873
BsoBI CYCGRG 2 cut(s) 244, 483
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 5 cut(s) 484, 490, 618, 820, 861
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 2 cut(s) 240, 358
BspACI CCGC 5 cut(s) 6, 235, 598, 608, 727
BspANI GGCC 4 cut(s) 5, 238, 782, 873
BspCNI CTCAG 2 cut(s) 52, 502
BspLI GGNNCC 4 cut(s) 377, 438, 713, 858
BspMI ACCTGC 1 cut(s) 560
BspPI GGATC 1 cut(s) 235
BspT104I TTCGAA 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 375
BsrDI GCAATG 3 cut(s) 163, 555, 837
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 1 cut(s) 498
BssECI CCNNGG 1 cut(s) 783
BssMI GATC 2 cut(s) 240, 358
BssNAI GTATAC 1 cut(s) 724
Bst1107I GTATAC 1 cut(s) 724
Bst2UI CCWGG 1 cut(s) 784
Bst4CI ACNGT 5 cut(s) 196, 262, 317, 352, 658
Bst6I CTCTTC 1 cut(s) 468
BstAUI TGTACA 1 cut(s) 252
BstBI TTCGAA 1 cut(s) 74
BstDEI CTNAG 3 cut(s) 39, 489, 741
BstF5I GGATG 2 cut(s) 134, 376
BstKTI GATC 2 cut(s) 243, 361
BstMBI GATC 2 cut(s) 240, 358
BstMCI CGRYCG 1 cut(s) 239
BstMWI GCNNNNNNNGC 6 cut(s) 197, 280, 566, 597, 824, 833
BstNI CCWGG 1 cut(s) 784
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 3 cut(s) 438, 708, 782
BstSFI CTRYAG 3 cut(s) 195, 592, 875
BstV1I GCAGC 3 cut(s) 99, 295, 689
BstV2I GAAGAC 2 cut(s) 165, 617
BstZ17I GTATAC 1 cut(s) 724
BstZI CGGCCG 1 cut(s) 236
BsuRI GGCC 4 cut(s) 5, 238, 782, 873
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 2 cut(s) 134, 376
BtsIMutI CAGTG 1 cut(s) 258
BveI ACCTGC 1 cut(s) 560
CaiI CAGNNNCTG 1 cut(s) 274
Cfr13I GGNCC 5 cut(s) 309, 436, 706, 712, 871
Csp6I GTAC 4 cut(s) 99, 253, 301, 376
CviAII CATG 2 cut(s) 169, 367
CviQI GTAC 4 cut(s) 99, 253, 301, 376
DdeI CTNAG 3 cut(s) 39, 489, 741
DpnI GATC 2 cut(s) 242, 360
DpnII GATC 2 cut(s) 240, 358
EaeI YGGCCR 3 cut(s) 3, 236, 780
EagI CGGCCG 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
EclXI CGGCCG 1 cut(s) 236
Eco24I GRGCYC 4 cut(s) 484, 490, 618, 861
Eco47I GGWCC 4 cut(s) 309, 436, 706, 712
Eco52I CGGCCG 1 cut(s) 236
Eco57I CTGAAG 2 cut(s) 165, 391
Eco88I CYCGRG 2 cut(s) 244, 483
EcoO109I RGGNCCY 3 cut(s) 309, 436, 712
EcoRII CCWGG 1 cut(s) 782
EcoT38I GRGCYC 4 cut(s) 484, 490, 618, 861
FaeI CATG 2 cut(s) 172, 370
FaqI GGGAC 1 cut(s) 725
FatI CATG 2 cut(s) 168, 366
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 6 cut(s) 6, 113, 236, 284, 598, 678
FokI GGATG 2 cut(s) 121, 383
FriOI GRGCYC 4 cut(s) 484, 490, 618, 861
Fsp4HI GCNGC 6 cut(s) 6, 113, 236, 284, 598, 678
FspBI CTAG 2 cut(s) 60, 381
GluI GCNGC 6 cut(s) 6, 113, 236, 284, 598, 678
HaeIII GGCC 4 cut(s) 5, 238, 782, 873
HapII CCGG 3 cut(s) 130, 440, 709
Hin1II CATG 2 cut(s) 172, 370
HinfI GANTC 1 cut(s) 93
HpaII CCGG 3 cut(s) 130, 440, 709
Hpy166II GTNNAC 3 cut(s) 253, 706, 724
Hpy188I TCNGA 4 cut(s) 82, 184, 410, 492
Hpy188III TCNNGA 2 cut(s) 244, 629
Hpy8I GTNNAC 3 cut(s) 253, 706, 724
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 3 cut(s) 521, 544, 817
HpyCH4III ACNGT 5 cut(s) 196, 262, 317, 352, 658
HpyCH4IV ACGT 1 cut(s) 845
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 280, 566, 597, 824, 833
HpyF3I CTNAG 3 cut(s) 39, 489, 741
HpySE526I ACGT 1 cut(s) 845
Hsp92II CATG 2 cut(s) 172, 370
KpnI GGTACC 1 cut(s) 379
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 2 cut(s) 240, 358
LmnI GCTCC 4 cut(s) 271, 442, 815, 856
Lsp1109I GCAGC 3 cut(s) 99, 295, 689
LweI GCATC 2 cut(s) 388, 448
MaeI CTAG 2 cut(s) 60, 381
MaeII ACGT 1 cut(s) 845
MaeIII GTNAC 3 cut(s) 10, 317, 346
MalI GATC 2 cut(s) 242, 360
MboI GATC 2 cut(s) 240, 358
MboII GAAGA 7 cut(s) 165, 397, 485, 617, 802, 805, 815
MhlI GDGCHC 5 cut(s) 484, 490, 618, 820, 861
MlsI TGGCCA 1 cut(s) 782
MluCI AATT 4 cut(s) 64, 139, 214, 512
MluNI TGGCCA 1 cut(s) 782
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 6 cut(s) 159, 291, 300, 382, 427, 532
Mox20I TGGCCA 1 cut(s) 782
MscI TGGCCA 1 cut(s) 782
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 782
MspI CCGG 3 cut(s) 130, 440, 709
MspR9I CCNGG 3 cut(s) 440, 710, 784
MvaI CCWGG 1 cut(s) 784
MwoI GCNNNNNNNGC 6 cut(s) 197, 280, 566, 597, 824, 833
NciI CCSGG 2 cut(s) 440, 710
NdeII GATC 2 cut(s) 240, 358
NlaIII CATG 2 cut(s) 172, 370
NlaIV GGNNCC 4 cut(s) 377, 438, 713, 858
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 1 cut(s) 172
NspV TTCGAA 1 cut(s) 74
PaqCI CACCTGC 1 cut(s) 560
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PfeI GAWTC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 868
PkrI GCNGC 6 cut(s) 7, 114, 237, 285, 599, 679
PpuMI RGGWCCY 3 cut(s) 309, 436, 712
Psp5II RGGWCCY 3 cut(s) 309, 436, 712
Psp6I CCWGG 1 cut(s) 782
PspGI CCWGG 1 cut(s) 782
PspN4I GGNNCC 4 cut(s) 377, 438, 713, 858
PspPI GGNCC 5 cut(s) 309, 436, 706, 712, 871
PspPPI RGGWCCY 3 cut(s) 309, 436, 712
PstNI CAGNNNCTG 1 cut(s) 274
RsaI GTAC 4 cut(s) 100, 254, 302, 377
RsaNI GTAC 4 cut(s) 99, 253, 301, 376
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 1 cut(s) 683
SatI GCNGC 6 cut(s) 6, 113, 236, 284, 598, 678
Sau3AI GATC 2 cut(s) 240, 358
Sau96I GGNCC 5 cut(s) 309, 436, 706, 712, 871
ScrFI CCNGG 3 cut(s) 440, 710, 784
SduI GDGCHC 5 cut(s) 484, 490, 618, 820, 861
SfaNI GCATC 2 cut(s) 388, 448
SfcI CTRYAG 3 cut(s) 195, 592, 875
SfuI TTCGAA 1 cut(s) 74
SinI GGWCC 4 cut(s) 309, 436, 706, 712
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 4 cut(s) 64, 139, 214, 512
SsiI CCGC 5 cut(s) 6, 235, 598, 608, 727
SspMI CTAG 2 cut(s) 60, 381
StyD4I CCNGG 3 cut(s) 438, 708, 782
TaaI ACNGT 5 cut(s) 196, 262, 317, 352, 658
TaiI ACGT 1 cut(s) 848
TaqI TCGA 2 cut(s) 74, 176
TasI AATT 4 cut(s) 64, 139, 214, 512
TatI WGTACW 1 cut(s) 252
TauI GCSGC 3 cut(s) 8, 238, 600
TfiI GAWTC 1 cut(s) 93
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 112, 283, 677
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 152, 846
TspGWI ACGGA 2 cut(s) 86, 507
TspRI CASTG 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 868
VpaK11BI GGWCC 4 cut(s) 309, 436, 706, 712
XapI RAATTY 3 cut(s) 64, 214, 512
XceI RCATGY 1 cut(s) 172
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmiI GTMKAC 1 cut(s) 723
XspI CTAG 2 cut(s) 60, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.