MD17G1089100.v1.1

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016063
Physical Location & Seq
Reverse (-)
7322015 .. 7322933
919 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1089100.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGGACTCTCTCATGTCCATTGCTTGACACTTGAAAGCCAATGCATTGAGATTCTATCAAACAGTAATTATTTTGCAGTTGATCTAAATCCATTTAACAATCTCAAATCTTTGGAGTTGCATACGTGTTTCAACAAGAATAATATACCTGGATTGGCGTGCATATTCAAGAGCTCTCCCATGTTGCACACTCTGATTCTCAATATTATAAATGATTACAAGATTGTAAGGAGAAGGAAGAAAAGTACTGGGAATCCGACCTTGAAGCCCTTCCTACAAAACCTTAAGGTGGTAAAGATTCATGGGTTTTTAGAGTACGAGAATGAAGTTAGTCTAACAAAGTTCTTGCTGAAGCATGGAAAGGCCTTGGAAGAAATGATTTTGTGCACTGGACACTGCAAAGCTAAATATTCTCTCCAGCGACAAAAGATTAGGTCACAGATGATGGGATTTTCTTGGGCTTCTTCTAATGCTAAAATTGCATTTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

164

Amino Acids

18.64

Weight (kDa)

9.56

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 27 - 142 1.3e-07 At1g61320/AtMIF1, LRR domain
FBD PF08387 91 - 128 1.6e-07 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016063)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03220
fragaria_vesca FvH4_6g44010
malus_domestica MD09G1101500.v1.1 MD17G1089100.v1.1
prunus_persica Prupe.3G224700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0160751
rosa_laevigata RLG00000021239
rosa_multiflora Rmu_sc0002773.1_g000009
rosa_roxburghii Rroxscaffold_2G00089400
rosa_rugosa Rorug02G0485700
rosa_samantha Rh2AG550700 Rh2BG565300 Rh2CG535100 Rh2DG574200
rosa_wichuraiana Rw2G045630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 2 cut(s) 247, 317
AfiI CCNNNNNNNGG 1 cut(s) 289
AflII CTTAAG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 125
AgsI TTSAA 4 cut(s) 35, 133, 169, 265
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 2 cut(s) 174, 404
AluI AGCT 2 cut(s) 174, 404
Alw21I GWGCWC 2 cut(s) 176, 389
Alw44I GTGCAC 1 cut(s) 385
AoxI GGCC 1 cut(s) 363
ApaLI GTGCAC 1 cut(s) 385
Asp700I GAANNNNTTC 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 176
Bbv12I GWGCWC 2 cut(s) 176, 389
BccI CCATC 1 cut(s) 439
BciT130I CCWGG 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 284
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 150
BmrFI CCNGG 1 cut(s) 150
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 401
BsaAI YACGTR 1 cut(s) 126
BsaBI GATNNNNATC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 366
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 2 cut(s) 253, 394
Bse3DI GCAATG 1 cut(s) 19
Bse8I GATNNNNATC 1 cut(s) 87
BseBI CCWGG 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 366
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 1 cut(s) 19
BseNI ACTGG 2 cut(s) 253, 394
BseSI GKGCMC 1 cut(s) 389
BshFI GGCC 1 cut(s) 365
BsiHKAI GWGCWC 2 cut(s) 176, 389
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 176, 389
Bsp143I GATC 1 cut(s) 82
BspANI GGCC 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 2 cut(s) 253, 394
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 65
BstAFI CTTAAG 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 160
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 389
BsuRI GGCC 1 cut(s) 365
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 3 cut(s) 387, 394, 487
Cac8I GCNNGC 1 cut(s) 160
Csp6I GTAC 2 cut(s) 246, 316
CviAII CATG 4 cut(s) 14, 181, 302, 356
CviJI RGCY 6 cut(s) 39, 174, 268, 365, 404, 461
CviKI_1 RGCY 6 cut(s) 39, 174, 268, 365, 404, 461
CviQI GTAC 2 cut(s) 246, 316
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 1 cut(s) 365
Eco24I GRGCYC 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 174
EcoRII CCWGG 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 4 cut(s) 17, 184, 305, 359
FaiI YATR 8 cut(s) 15, 123, 146, 164, 182, 209, 303, 357
FaqI GGGAC 1 cut(s) 18
FatI CATG 4 cut(s) 13, 180, 301, 355
FriOI GRGCYC 1 cut(s) 176
GsuI CTGGAG 1 cut(s) 401
HaeIII GGCC 1 cut(s) 365
Hin1II CATG 4 cut(s) 17, 184, 305, 359
HinfI GANTC 5 cut(s) 6, 52, 196, 253, 298
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 2 cut(s) 195, 258
Hpy188III TCNNGA 1 cut(s) 169
Hpy8I GTNNAC 1 cut(s) 387
HpyAV CCTTC 2 cut(s) 228, 280
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 8 cut(s) 45, 77, 121, 162, 187, 387, 399, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 4 cut(s) 17, 184, 305, 359
Kzo9I GATC 1 cut(s) 82
LpnPI CCDG 5 cut(s) 135, 162, 234, 375, 431
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 1 cut(s) 435
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 3 cut(s) 250, 383, 456
MhlI GDGCHC 2 cut(s) 176, 389
MluCI AATT 2 cut(s) 67, 477
MmeI TCCRAC 1 cut(s) 281
Mph1103I ATGCAT 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 269
MseI TTAA 2 cut(s) 96, 285
MspCI CTTAAG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 150
MwoI GCNNNNNNNGC 1 cut(s) 479
NdeII GATC 1 cut(s) 82
NlaIII CATG 4 cut(s) 17, 184, 305, 359
NmuCI GTSAC 1 cut(s) 435
NsiI ATGCAT 1 cut(s) 47
PceI AGGCCT 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 4 cut(s) 52, 196, 253, 298
Ppu21I YACGTR 1 cut(s) 126
PsiI TTATAA 1 cut(s) 209
Psp124BI GAGCTC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
RsaI GTAC 2 cut(s) 247, 317
RsaNI GTAC 2 cut(s) 246, 316
SacI GAGCTC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 96, 285
Sau3AI GATC 1 cut(s) 82
ScaI AGTACT 1 cut(s) 247
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 2 cut(s) 176, 389
SetI ASST 8 cut(s) 128, 151, 176, 263, 285, 291, 406, 437
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 2 cut(s) 67, 477
SseBI AGGCCT 1 cut(s) 365
SspI AATATT 2 cut(s) 205, 410
SstI GAGCTC 1 cut(s) 176
StuI AGGCCT 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 148
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 1 cut(s) 128
TasI AATT 2 cut(s) 67, 477
TatI WGTACW 1 cut(s) 245
TfiI GAWTC 4 cut(s) 52, 196, 253, 298
Tru1I TTAA 2 cut(s) 96, 285
Tru9I TTAA 2 cut(s) 96, 285
TscAI CASTG 2 cut(s) 394, 401
TseFI GTSAC 1 cut(s) 435
Tsp45I GTSAC 1 cut(s) 435
TspDTI ATGAA 2 cut(s) 290, 339
TspRI CASTG 2 cut(s) 394, 401
Vha464I CTTAAG 1 cut(s) 284
VneI GTGCAC 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 269
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.