Prupe.3G224700_v2.0.a1

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0016063
Physical Location & Seq
Forward (+)
22402900 .. 22405101
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G224700.1

Sequence Viewer

Length: 1572 bp
ATGGAAACAAGATCCGCTGCCAAGAAAAGAAAGCTCCTCATTTTATACAACCAGAAGGATCAAGTTGACGACGATGACGAAAGCGAGAAGAGCAACAGAAGCAGAAGCGTTGACCGCATCAGTGACCTCCCAGATTACGTTCTCCACCACATCCTCTTCCTCCTACCCATCAAAACCGTCGCCCAAACCAGCGTCTTGTCAAAACGATGGCGTTCTATCTGGTCCTCGTTCCCGGACCTCGACTTCACCACCCTCACTCGAACCCCCACAAAGCCACATTCTTCTTCTTCATCAAAATCGCTCTCTCAGAATTCCAAAGAAACAGACTTCATCACGCAAGTCCTGACTCTCCGCGATAAGCACTCCGATATTCGGATCCTTCGGTTCGGTTCCCGGTTGAGCTTCTCCCGTCTCAATGGCCTGATCCGCCTGGCCATCCGGCGCAATGTGCAGGAACTGGACGTGGAGGTTGCCACAGAGGATTATTTCAACTTCCCTAGATGCGTCATCGCCAGCGACTCTCTCCGCATTTTCAAGTTGAAATCGCGCTACCCAGGCTTCAGATTGCCCCCTTCATCTGTGATGACGAGCGGATTCAAATCCCTCCACTCGTTGTCGCTTTCACGCATTGTCTTATACAACCAGCCTTCTCTCTGCGACATGTTCGGAGAGTCTGCTTTCCCTATGCTCAAGAAACTGAGCTTGGACGCTTGTTTCGGGTTGAAGTACCTTCGGATTGGGTGCAGGGCGCTTGAGGATTTGACCCTGGAGAACTGCTTTCAGTTACAAGGGTTGGATGTTTTGGGTGGGAAGTTGGAGAGGTTGAGAATTGTGAGGTGTTTTGATGCTTATGCTGATAAGAGTTGGGTGAAGATTGGTGGTCCAAGACTTAGAATTATTCTTTGGGAAAACAATGCTATTACTGATAGCTCTTCTCTGGACAATTTGAGCTCTCTGCAAGAGGCCTCCATTGGTTTCTTTTTGCTCCAGGAAGATATTAGTGTGGCCAAGCTTCAGAGTGTGTCCAACCTTTTTATGGGACTCTATCATGCCCATTGCTTGACACTTGATACGCAATGTATTGAGATTCTGTCAAACAGTAATTACTTTGCGGTTTATCTGCATCCATTCAACAATCTCAAGTCTTTGGAGTTGCATACGGGTTTCAACAAGAACAATGTCCCTGGATTGGCGTGCATATTCAGGAGCTCTCCCATGCTTCACACTCTGACCCTCAATATTATTAACGATTACAAGATTGAAAGGAGACAATGGAATAAGGATTTGTGGGAAATGTCCAATTCTGAGGAAGAAAAATTCTGGGAATCGCAAACGCAAGCCTTGAGGTCGTTTTTACAAAACCTTACGGTAGTAAAGATCCAGGGGTTTTTAGAGTGCGAGAATGAAGTGAGTCTAGCAAAGTTCTTGCTGAAGCATGGAAAGGCCTTGCAGGAAATGATTCTGTGCACAGGACACTGCAAAGCTAGATATTCTCTCCAGCGACAAAAGATTAGGTCACAGATGATGGGATTCTCTTGGGCTTCTTCTAATGCCAAAATTGCATTTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

524

Amino Acids

60.02

Weight (kDa)

9.17

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016063)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03220
fragaria_vesca FvH4_6g44010
malus_domestica MD09G1101500.v1.1 MD17G1089100.v1.1
prunus_persica Prupe.3G224700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0160751
rosa_laevigata RLG00000021239
rosa_multiflora Rmu_sc0002773.1_g000009
rosa_roxburghii Rroxscaffold_2G00089400
rosa_rugosa Rorug02G0485700
rosa_samantha Rh2AG550700 Rh2BG565300 Rh2CG535100 Rh2DG574200
rosa_wichuraiana Rw2G045630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 591
AccII CGCG 2 cut(s) 354, 547
AciI CCGC 7 cut(s) 15, 115, 352, 427, 526, 591, 1112
AclWI GGATC 6 cut(s) 6, 66, 370, 383, 418, 1372
AcoI YGGCCR 2 cut(s) 432, 1005
AcsI RAATTY 2 cut(s) 310, 1316
AcuI CTGAAG 3 cut(s) 544, 998, 1451
AfaI GTAC 1 cut(s) 728
AfiI CCNNNNNNNGG 3 cut(s) 372, 1036, 1189
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 8 cut(s) 490, 535, 541, 598, 724, 1132, 1168, 1262
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 6 cut(s) 429, 553, 765, 987, 1183, 1380
AjuI GAANNNNNNNTTGG 4 cut(s) 686, 718, 886, 918
AluBI AGCT 8 cut(s) 34, 402, 702, 930, 951, 1012, 1209, 1484
AluI AGCT 8 cut(s) 34, 402, 702, 930, 951, 1012, 1209, 1484
Alw21I GWGCWC 3 cut(s) 953, 1211, 1469
Alw26I GTCTC 2 cut(s) 416, 1261
Alw44I GTGCAC 1 cut(s) 1465
AlwI GGATC 6 cut(s) 6, 66, 370, 383, 418, 1372
AlwNI CAGNNNCTG 1 cut(s) 457
AoxI GGCC 5 cut(s) 418, 432, 963, 1005, 1443
ApaLI GTGCAC 1 cut(s) 1465
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 2 cut(s) 310, 1316
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
Asp700I GAANNNNTTC 1 cut(s) 1458
AspLEI GCGC 3 cut(s) 444, 549, 751
AspS9I GGNCC 3 cut(s) 222, 235, 881
AsuC2I CCSGG 2 cut(s) 233, 394
AsuHPI GGTGA 2 cut(s) 238, 880
AvaII GGWCC 3 cut(s) 222, 235, 881
BaeGI GKGCMC 1 cut(s) 1469
BalI TGGCCA 2 cut(s) 434, 1007
BamHI GGATCC 1 cut(s) 375
BanII GRGCYC 2 cut(s) 953, 1211
Bbv12I GWGCWC 3 cut(s) 953, 1211, 1469
BbvI GCAGC 1 cut(s) 4
BccI CCATC 4 cut(s) 176, 201, 443, 1519
BciT130I CCWGG 6 cut(s) 431, 555, 767, 989, 1185, 1382
BcnI CCSGG 2 cut(s) 233, 394
BcoDI GTCTC 2 cut(s) 416, 1261
BfaI CTAG 3 cut(s) 498, 1415, 1485
BfoI RGCGCY 1 cut(s) 752
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 8 cut(s) 233, 394, 431, 555, 767, 989, 1185, 1382
Bme18I GGWCC 3 cut(s) 222, 235, 881
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 3 cut(s) 222, 235, 881
BmiI GGNNCC 2 cut(s) 377, 391
BmrFI CCNGG 8 cut(s) 233, 394, 431, 555, 767, 989, 1185, 1382
BmsI GCATC 4 cut(s) 126, 491, 835, 1132
BpmI CTGGAG 3 cut(s) 788, 971, 1481
BpuEI CTTGAG 4 cut(s) 674, 773, 1124, 1363
BpuMI CCSGG 2 cut(s) 233, 394
BsaBI GATNNNNATC 1 cut(s) 598
BsaJI CCNNGG 4 cut(s) 553, 765, 1183, 1381
Bsc4I CCNNNNNNNGG 3 cut(s) 372, 1036, 1189
Bse1I ACTGG 1 cut(s) 462
Bse3DI GCAATG 3 cut(s) 451, 1054, 1082
Bse8I GATNNNNATC 1 cut(s) 598
BseBI CCWGG 6 cut(s) 431, 555, 767, 989, 1185, 1382
BseDI CCNNGG 4 cut(s) 553, 765, 1183, 1381
BseGI GGATG 4 cut(s) 150, 435, 802, 1123
BseJI GATNNNNATC 1 cut(s) 598
BseLI CCNNNNNNNGG 3 cut(s) 372, 1036, 1189
BseMI GCAATG 3 cut(s) 451, 1054, 1082
BseMII CTCAG 3 cut(s) 320, 689, 1296
BseNI ACTGG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 1469
BseXI GCAGC 1 cut(s) 4
BsgI GTGCAG 2 cut(s) 470, 763
Bsh1236I CGCG 2 cut(s) 354, 547
BshFI GGCC 5 cut(s) 420, 434, 965, 1007, 1445
BsiHKAI GWGCWC 3 cut(s) 953, 1211, 1469
BsiSI CCGG 3 cut(s) 233, 394, 439
BslFI GGGAC 2 cut(s) 1053, 1166
BslI CCNNNNNNNGG 3 cut(s) 372, 1036, 1189
BsmAI GTCTC 2 cut(s) 416, 1261
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 2 cut(s) 1053, 1166
BsnI GGCC 5 cut(s) 420, 434, 965, 1007, 1445
Bsp1286I GDGCHC 3 cut(s) 953, 1211, 1469
Bsp143I GATC 5 cut(s) 11, 58, 375, 423, 1377
BspACI CCGC 7 cut(s) 15, 115, 352, 427, 526, 591, 1112
BspANI GGCC 5 cut(s) 420, 434, 965, 1007, 1445
BspCNI CTCAG 3 cut(s) 319, 690, 1297
BspFNI CGCG 2 cut(s) 354, 547
BspLI GGNNCC 2 cut(s) 377, 391
BspPI GGATC 6 cut(s) 6, 66, 370, 383, 418, 1372
BspQI GCTCTTC 2 cut(s) 83, 937
BsrBI CCGCTC 1 cut(s) 591
BsrDI GCAATG 3 cut(s) 451, 1054, 1082
BsrI ACTGG 1 cut(s) 462
BssECI CCNNGG 4 cut(s) 553, 765, 1183, 1381
BssMI GATC 5 cut(s) 11, 58, 375, 423, 1377
Bst2UI CCWGG 6 cut(s) 431, 555, 767, 989, 1185, 1382
Bst4CI ACNGT 3 cut(s) 178, 1100, 1369
Bst6I CTCTTC 3 cut(s) 83, 161, 937
BstC8I GCNNGC 3 cut(s) 514, 1195, 1338
BstDEI CTNAG 4 cut(s) 306, 698, 890, 1305
BstF5I GGATG 4 cut(s) 150, 435, 802, 1123
BstFNI CGCG 2 cut(s) 354, 547
BstH2I RGCGCY 1 cut(s) 752
BstHHI GCGC 3 cut(s) 444, 549, 751
BstKTI GATC 5 cut(s) 14, 61, 378, 426, 1380
BstMAI GTCTC 2 cut(s) 416, 1261
BstMBI GATC 5 cut(s) 11, 58, 375, 423, 1377
BstMWI GCNNNNNNNGC 7 cut(s) 90, 99, 114, 426, 448, 555, 1559
BstNI CCWGG 6 cut(s) 431, 555, 767, 989, 1185, 1382
BstNSI RCATGY 1 cut(s) 664
BstSCI CCNGG 8 cut(s) 231, 392, 429, 553, 765, 987, 1183, 1380
BstSLI GKGCMC 1 cut(s) 1469
BstUI CGCG 2 cut(s) 354, 547
BstV1I GCAGC 1 cut(s) 4
BstX2I RGATCY 3 cut(s) 11, 375, 1377
BstYI RGATCY 3 cut(s) 11, 375, 1377
BsuRI GGCC 5 cut(s) 420, 434, 965, 1007, 1445
BtgZI GCGATG 1 cut(s) 493
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 4 cut(s) 150, 435, 802, 1123
BtsI GCAGTG 1 cut(s) 1474
BtsIMutI CAGTG 2 cut(s) 127, 1474
Cac8I GCNNGC 3 cut(s) 514, 1195, 1338
CaiI CAGNNNCTG 1 cut(s) 457
CfoI GCGC 3 cut(s) 444, 549, 751
Cfr13I GGNCC 3 cut(s) 222, 235, 881
CseI GACGC 3 cut(s) 181, 493, 716
Csp6I GTAC 1 cut(s) 727
CviAII CATG 4 cut(s) 661, 1049, 1216, 1436
CviQI GTAC 1 cut(s) 727
DdeI CTNAG 4 cut(s) 306, 698, 890, 1305
DpnI GATC 5 cut(s) 13, 60, 377, 425, 1379
DpnII GATC 5 cut(s) 11, 58, 375, 423, 1377
EaeI YGGCCR 2 cut(s) 432, 1005
Eam1104I CTCTTC 3 cut(s) 83, 161, 937
EarI CTCTTC 3 cut(s) 83, 161, 937
EciI GGCGGA 1 cut(s) 416
Ecl136II GAGCTC 2 cut(s) 951, 1209
Eco147I AGGCCT 2 cut(s) 965, 1445
Eco24I GRGCYC 2 cut(s) 953, 1211
Eco47I GGWCC 3 cut(s) 222, 235, 881
Eco53kI GAGCTC 2 cut(s) 951, 1209
Eco57I CTGAAG 3 cut(s) 544, 998, 1451
EcoICRI GAGCTC 2 cut(s) 951, 1209
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 6 cut(s) 429, 553, 765, 987, 1183, 1380
EcoT38I GRGCYC 2 cut(s) 953, 1211
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 4 cut(s) 664, 1052, 1219, 1439
FaqI GGGAC 2 cut(s) 1053, 1166
FatI CATG 4 cut(s) 660, 1048, 1215, 1435
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 4 cut(s) 137, 422, 809, 1110
FriOI GRGCYC 2 cut(s) 953, 1211
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 3 cut(s) 498, 1415, 1485
GlaI GCGC 3 cut(s) 443, 548, 750
GluI GCNGC 1 cut(s) 18
GsuI CTGGAG 3 cut(s) 788, 971, 1481
HaeII RGCGCY 1 cut(s) 752
HaeIII GGCC 5 cut(s) 420, 434, 965, 1007, 1445
HapII CCGG 3 cut(s) 233, 394, 439
HgaI GACGC 3 cut(s) 181, 493, 716
HhaI GCGC 3 cut(s) 444, 549, 751
Hin1II CATG 4 cut(s) 664, 1052, 1219, 1439
Hin6I GCGC 3 cut(s) 442, 547, 749
HinP1I GCGC 3 cut(s) 442, 547, 749
HincII GTYRAC 2 cut(s) 67, 112
HindII GTYRAC 2 cut(s) 67, 112
HindIII AAGCTT 1 cut(s) 1010
HpaII CCGG 3 cut(s) 233, 394, 439
HphI GGTGA 2 cut(s) 238, 880
Hpy166II GTNNAC 3 cut(s) 67, 112, 1467
Hpy188I TCNGA 9 cut(s) 309, 367, 375, 563, 668, 735, 1017, 1230, 1306
Hpy188III TCNNGA 4 cut(s) 343, 691, 938, 1204
Hpy8I GTNNAC 3 cut(s) 67, 112, 1467
Hpy99I CGWCG 2 cut(s) 74, 182
HpyAV CCTTC 5 cut(s) 49, 389, 582, 657, 740
HpyCH4III ACNGT 3 cut(s) 178, 1100, 1369
HpyCH4IV ACGT 2 cut(s) 138, 462
HpyF10VI GCNNNNNNNGC 7 cut(s) 90, 99, 114, 426, 448, 555, 1559
HpyF3I CTNAG 4 cut(s) 306, 698, 890, 1305
HpySE526I ACGT 2 cut(s) 138, 462
Hsp92II CATG 4 cut(s) 664, 1052, 1219, 1439
HspAI GCGC 3 cut(s) 442, 547, 749
Kzo9I GATC 5 cut(s) 11, 58, 375, 423, 1377
LguI GCTCTTC 2 cut(s) 83, 937
LmnI GCTCC 3 cut(s) 39, 990, 1206
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 4 cut(s) 126, 491, 835, 1132
MaeI CTAG 3 cut(s) 498, 1415, 1485
MaeII ACGT 2 cut(s) 138, 462
MaeIII GTNAC 3 cut(s) 122, 783, 1515
MalI GATC 5 cut(s) 13, 60, 377, 425, 1379
MbiI CCGCTC 1 cut(s) 591
MboI GATC 5 cut(s) 11, 58, 375, 423, 1377
MflI RGATCY 3 cut(s) 11, 375, 1377
MhlI GDGCHC 3 cut(s) 953, 1211, 1469
MlsI TGGCCA 2 cut(s) 434, 1007
MluCI AATT 8 cut(s) 310, 828, 894, 943, 1102, 1300, 1316, 1557
MluNI TGGCCA 2 cut(s) 434, 1007
MlyI GAGTC 5 cut(s) 340, 512, 680, 1035, 1420
MmeI TCCRAC 3 cut(s) 774, 795, 1050
Mox20I TGGCCA 2 cut(s) 434, 1007
MroXI GAANNNNTTC 1 cut(s) 1458
MscI TGGCCA 2 cut(s) 434, 1007
MseI TTAA 1 cut(s) 1245
Msp20I TGGCCA 2 cut(s) 434, 1007
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 233, 394, 439
MspR9I CCNGG 8 cut(s) 233, 394, 431, 555, 767, 989, 1185, 1382
MvaI CCWGG 6 cut(s) 431, 555, 767, 989, 1185, 1382
MvnI CGCG 2 cut(s) 354, 547
MwoI GCNNNNNNNGC 7 cut(s) 90, 99, 114, 426, 448, 555, 1559
NciI CCSGG 2 cut(s) 233, 394
NdeII GATC 5 cut(s) 11, 58, 375, 423, 1377
NlaIII CATG 4 cut(s) 664, 1052, 1219, 1439
NlaIV GGNNCC 2 cut(s) 377, 391
NmuCI GTSAC 2 cut(s) 122, 1515
NspI RCATGY 1 cut(s) 664
PceI AGGCCT 2 cut(s) 965, 1445
PciI ACATGT 1 cut(s) 660
PciSI GCTCTTC 2 cut(s) 83, 937
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 1458
PfeI GAWTC 5 cut(s) 594, 1087, 1325, 1459, 1530
PfoI TCCNGGA 2 cut(s) 231, 987
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 5 cut(s) 340, 512, 679, 1035, 1419
PpsI GAGTC 5 cut(s) 340, 512, 679, 1035, 1419
PscI ACATGT 1 cut(s) 660
Psp124BI GAGCTC 2 cut(s) 953, 1211
Psp6I CCWGG 6 cut(s) 429, 553, 765, 987, 1183, 1380
PspGI CCWGG 6 cut(s) 429, 553, 765, 987, 1183, 1380
PspN4I GGNNCC 2 cut(s) 377, 391
PspPI GGNCC 3 cut(s) 222, 235, 881
PstNI CAGNNNCTG 1 cut(s) 457
PsuI RGATCY 3 cut(s) 11, 375, 1377
RsaI GTAC 1 cut(s) 728
RsaNI GTAC 1 cut(s) 727
SacI GAGCTC 2 cut(s) 953, 1211
SapI GCTCTTC 2 cut(s) 83, 937
SaqAI TTAA 1 cut(s) 1245
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 5 cut(s) 11, 58, 375, 423, 1377
Sau96I GGNCC 3 cut(s) 222, 235, 881
SchI GAGTC 5 cut(s) 340, 512, 680, 1035, 1420
ScrFI CCNGG 8 cut(s) 233, 394, 431, 555, 767, 989, 1185, 1382
SduI GDGCHC 3 cut(s) 953, 1211, 1469
SfaNI GCATC 4 cut(s) 126, 491, 835, 1132
SinI GGWCC 3 cut(s) 222, 235, 881
SmlI CTYRAG 4 cut(s) 689, 752, 1139, 1342
SmoI CTYRAG 4 cut(s) 689, 752, 1139, 1342
Sse9I AATT 8 cut(s) 310, 828, 894, 943, 1102, 1300, 1316, 1557
SseBI AGGCCT 2 cut(s) 965, 1445
SsiI CCGC 7 cut(s) 15, 115, 352, 427, 526, 591, 1112
SspI AATATT 1 cut(s) 1240
SspMI CTAG 3 cut(s) 498, 1415, 1485
SstI GAGCTC 2 cut(s) 953, 1211
StuI AGGCCT 2 cut(s) 965, 1445
StyD4I CCNGG 8 cut(s) 231, 392, 429, 553, 765, 987, 1183, 1380
TaaI ACNGT 3 cut(s) 178, 1100, 1369
TaiI ACGT 2 cut(s) 141, 465
TaqI TCGA 2 cut(s) 240, 259
TasI AATT 8 cut(s) 310, 828, 894, 943, 1102, 1300, 1316, 1557
TfiI GAWTC 5 cut(s) 594, 1087, 1325, 1459, 1530
Tru1I TTAA 1 cut(s) 1245
Tru9I TTAA 1 cut(s) 1245
TscAI CASTG 2 cut(s) 127, 1481
TseFI GTSAC 2 cut(s) 122, 1515
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 2 cut(s) 122, 1515
TspDTI ATGAA 4 cut(s) 279, 319, 564, 1419
TspRI CASTG 2 cut(s) 127, 1481
VneI GTGCAC 1 cut(s) 1465
VpaK11BI GGWCC 3 cut(s) 222, 235, 881
XapI RAATTY 2 cut(s) 310, 1316
XceI RCATGY 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XmnI GAANNNNTTC 1 cut(s) 1458
XspI CTAG 3 cut(s) 498, 1415, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.