Rh2CG535100

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016063
Physical Location & Seq
Forward (+)
71105881 .. 71108384
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG535100.1

Sequence Viewer

Length: 1533 bp
ATGGAAACAAGATCTGCCGCTGCCAAGAAGAAAAAGCTTTTCTTCTGCAAAGAAAATGAACACAGCCCCGATCACCACAACAATATTGACCGCATCACCGAACTCCCCGACGCCGTTCTCCACCACATCCTCTTCCTCCTCCCCATCAAAACCGTCGCGCAAACCAGCGTCTTGTCAAAACGGTGGCGTTCGCTCTGGTCGTCCTTCCCCGACCTCGACTTCACAACCCTAAACCCATTTCCGAAACCATGTTCCTCATCCAAGTCACATTCTCTGAATTGCAAAGAGTCCGACTTTATCACTCAGGTCCTCAACAACCGAGACAAGCACTCCGATATTCGGATTCTCCGGTTCCGGGCCCGGCTCAGCTTTTCACGTCTCAACGGTCTGATTCGGCTCGCAATCAGACACAAGGTGCAGGTGCTCGACGTGGAGGTTTTCACAGAGGATTACTTCAACTTCCCTAGATGTGTCATCGCCAGCGACTCATTAAGGGTTTTCAAGTTAAAGTCCCGGAGACCCGGTTTCCGGTTACCACCTTCTTCTGTAATGACAAGCGGTTTCCGATCTCTTCAGTCTTTATCGCTTTCCCGGATCAGTCTATGCAACCAGCCCTCTCTCTCCGATATGTTCTCCGAGTCGTCCTTCCTTCTGCTCAAGAAACTGAGCCTCGACACGTGTTTTGGGTTGAAGCATCTTAGGGTTGGGTGCCGTGCGCTGCAAGACTTAACCCTAGAGAACTGTTTCGAGCTGCAAGGGCTGGATGTTTCGGGTTCGAAATTGGAGAGGTTGCGGATAGCGAAATGTTTTGATGGGTACATTGAGAAGAGTTGGGTGAAGATTGATGCGCCGAGGCTTGGAGTGGTTTTGTGGGAACACAATGCTCTTAGTGATAATATCTGTATAGAGAATCTGAGTTACTTGCATAAGGCCTCCATTGGTTTGCTCCATGAAGATATTAGTGTTGCAAAGCTTCAAAGTGTGTCCAATCTTTTTTCGGCTCTCTCTCTTGCACATTGCTTGACACTCGAAAGCAATTGTATTGAGATTCTATCAAACAACAATTATTTTGCAGTTTATTTGCATCAGTTTAGCAATCTGAAATCTTTGGAGTTGCACACGGGTTTCAAGAAGAACAATGTCCCTGGATTAGCATCCATATTCAGGAGTTGTCCTATGCTTCACACTTTGATTCTCAACATTATTAACGATTTCAAAACTGAAAGGAGACAATGGAACAAGGATTTGTGGGAAATGTCTAACTCAGAGGAAGAGAAATACTGGGAATCCCAAACCCTAAACTTGAAGTTGTTCTTGCATCACCTTAAGATAGTGAAGATTCTTGGGTTTTTAGAGTGCGAGAATGAAGTGAGCCTGGCAAAGTTCTTGCTCAAACATGGAAAGGCCTTGGAAGAAATGATTTTGTCCACCGGGCACTGCAAAGCTAGACACTCTCTTCAGAGACAAAAGATTAGGTCACAGATGATGGGATTTTCTTGGGCTTCTTCTAATGCTAAAATTACATTTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

510

Amino Acids

58.59

Weight (kDa)

9.28

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 32 - 70 3.2e-08 F-box domain
LRR_At1g61320_AtMIF1 PF23622 121 - 267 5.7e-10 At1g61320/AtMIF1, LRR domain
LRR_At1g61320_AtMIF1 PF23622 237 - 490 9.9e-12 At1g61320/AtMIF1, LRR domain
FBD PF08387 438 - 475 3.7e-07 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016063)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03220
fragaria_vesca FvH4_6g44010
malus_domestica MD09G1101500.v1.1 MD17G1089100.v1.1
prunus_persica Prupe.3G224700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0160751
rosa_laevigata RLG00000021239
rosa_multiflora Rmu_sc0002773.1_g000009
rosa_roxburghii Rroxscaffold_2G00089400
rosa_rugosa Rorug02G0485700
rosa_samantha Rh2AG550700 Rh2BG565300 Rh2CG535100 Rh2DG574200
rosa_wichuraiana Rw2G045630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 409
Acc36I ACCTGC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 708
AccII CGCG 1 cut(s) 158
AciI CCGC 4 cut(s) 18, 91, 558, 793
AclWI GGATC 1 cut(s) 602
AcuI CTGAAG 2 cut(s) 557, 1442
AcvI CACGTG 1 cut(s) 678
AcyI GRCGYC 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 1 cut(s) 818
AfiI CCNNNNNNNGG 5 cut(s) 339, 355, 528, 857, 1164
AflII CTTAAG 1 cut(s) 1325
AflIII ACRYGT 2 cut(s) 675, 677
AgsI TTSAA 7 cut(s) 457, 502, 691, 977, 1129, 1216, 1306
AjiI CACGTC 2 cut(s) 377, 430
AjnI CCWGG 2 cut(s) 1144, 1374
AluBI AGCT 5 cut(s) 37, 369, 751, 973, 1445
AluI AGCT 5 cut(s) 37, 369, 751, 973, 1445
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 5 cut(s) 315, 383, 511, 1222, 1456
AlwI GGATC 1 cut(s) 602
AoxI GGCC 3 cut(s) 357, 930, 1404
ApaI GGGCCC 1 cut(s) 361
ApeKI GCWGC 3 cut(s) 20, 718, 751
ArsI GACNNNNNNTTYG 2 cut(s) 665, 697
Asp700I GAANNNNTTC 2 cut(s) 743, 1310
AspLEI GCGC 3 cut(s) 160, 718, 850
AspS9I GGNCC 3 cut(s) 307, 357, 358
AsuC2I CCSGG 6 cut(s) 356, 361, 514, 522, 592, 1432
AsuHPI GGTGA 4 cut(s) 65, 88, 847, 1313
AsuII TTCGAA 1 cut(s) 776
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 2 cut(s) 361, 1437
BanI GGYRCC 1 cut(s) 708
BanII GRGCYC 1 cut(s) 361
BbrPI CACGTG 1 cut(s) 678
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 3 cut(s) 7, 705, 738
BccI CCATC 3 cut(s) 152, 806, 1480
BceAI ACGGC 2 cut(s) 98, 696
BciT130I CCWGG 2 cut(s) 1146, 1376
BcnI CCSGG 6 cut(s) 356, 361, 514, 522, 592, 1432
BcoDI GTCTC 5 cut(s) 315, 383, 511, 1222, 1456
BfaI CTAG 3 cut(s) 465, 734, 1446
BfrI CTTAAG 1 cut(s) 1325
BfuAI ACCTGC 1 cut(s) 409
BglII AGATCT 1 cut(s) 11
BisI GCNGC 4 cut(s) 18, 21, 719, 752
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 4 cut(s) 19, 22, 720, 753
Bme1390I CCNGG 8 cut(s) 356, 361, 514, 522, 592, 1146, 1376, 1432
Bme18I GGWCC 1 cut(s) 307
BmgBI CACGTC 2 cut(s) 377, 430
BmgT120I GGNCC 3 cut(s) 307, 357, 358
BmiI GGNNCC 3 cut(s) 353, 359, 710
BmrFI CCNGG 8 cut(s) 356, 361, 514, 522, 592, 1146, 1376, 1432
BmrI ACTGGG 1 cut(s) 1291
BmsI GCATC 6 cut(s) 102, 703, 835, 1093, 1163, 1327
BmuI ACTGGG 1 cut(s) 1291
Bpu1102I GCTNAGC 1 cut(s) 365
Bpu14I TTCGAA 1 cut(s) 776
BpuEI CTTGAG 1 cut(s) 641
BpuMI CCSGG 6 cut(s) 356, 361, 514, 522, 592, 1432
BsaAI YACGTR 1 cut(s) 678
BsaBI GATNNNNATC 1 cut(s) 1153
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 511
BsaJI CCNNGG 3 cut(s) 851, 1144, 1407
BsaWI WCCGGW 2 cut(s) 348, 528
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 508, 538
Bsc4I CCNNNNNNNGG 5 cut(s) 339, 355, 528, 857, 1164
Bse1I ACTGG 1 cut(s) 1286
Bse3DI GCAATG 1 cut(s) 1015
Bse8I GATNNNNATC 1 cut(s) 1153
BseBI CCWGG 2 cut(s) 1146, 1376
BseDI CCNNGG 3 cut(s) 851, 1144, 1407
BseGI GGATG 4 cut(s) 126, 257, 769, 1154
BseJI GATNNNNATC 1 cut(s) 1153
BseLI CCNNNNNNNGG 5 cut(s) 339, 355, 528, 857, 1164
BseMI GCAATG 1 cut(s) 1015
BseMII CTCAG 5 cut(s) 317, 379, 656, 905, 1278
BseNI ACTGG 1 cut(s) 1286
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 2 cut(s) 361, 1437
BseXI GCAGC 3 cut(s) 7, 705, 738
BsgI GTGCAG 1 cut(s) 437
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 3 cut(s) 359, 932, 1406
BshNI GGYRCC 1 cut(s) 708
BsiHKAI GWGCWC 1 cut(s) 426
BsiSI CCGG 8 cut(s) 349, 355, 361, 514, 522, 529, 592, 1431
BslFI GGGAC 2 cut(s) 496, 1127
BslI CCNNNNNNNGG 5 cut(s) 339, 355, 528, 857, 1164
BsmAI GTCTC 5 cut(s) 315, 383, 511, 1222, 1456
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 496, 1127
BsnI GGCC 3 cut(s) 359, 932, 1406
Bso31I GGTCTC 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 776
Bsp120I GGGCCC 1 cut(s) 357
Bsp1286I GDGCHC 3 cut(s) 361, 426, 1437
Bsp143I GATC 4 cut(s) 11, 70, 566, 594
Bsp1720I GCTNAGC 1 cut(s) 365
BspACI CCGC 4 cut(s) 18, 91, 558, 793
BspANI GGCC 3 cut(s) 359, 932, 1406
BspCNI CTCAG 5 cut(s) 316, 378, 657, 906, 1277
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 3 cut(s) 353, 359, 710
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 1 cut(s) 602
BspT104I TTCGAA 1 cut(s) 776
BspT107I GGYRCC 1 cut(s) 708
BspTI CTTAAG 1 cut(s) 1325
BspTNI GGTCTC 1 cut(s) 511
BsrDI GCAATG 1 cut(s) 1015
BsrI ACTGG 1 cut(s) 1286
BssECI CCNNGG 3 cut(s) 851, 1144, 1407
BssMI GATC 4 cut(s) 11, 70, 566, 594
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 1 cut(s) 1407
Bst2UI CCWGG 2 cut(s) 1146, 1376
Bst4CI ACNGT 4 cut(s) 154, 183, 386, 743
Bst6I CTCTTC 5 cut(s) 137, 576, 821, 1266, 1461
BstACI GRCGYC 1 cut(s) 111
BstAFI CTTAAG 1 cut(s) 1325
BstBAI YACGTR 1 cut(s) 678
BstBI TTCGAA 1 cut(s) 776
BstC8I GCNNGC 2 cut(s) 399, 481
BstDEI CTNAG 7 cut(s) 303, 365, 665, 698, 887, 914, 1264
BstEII GGTNACC 1 cut(s) 531
BstF5I GGATG 4 cut(s) 126, 257, 769, 1154
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 3 cut(s) 160, 718, 850
BstKTI GATC 4 cut(s) 14, 73, 569, 597
BstMAI GTCTC 5 cut(s) 315, 383, 511, 1222, 1456
BstMBI GATC 4 cut(s) 11, 70, 566, 594
BstMWI GCNNNNNNNGC 1 cut(s) 757
BstNI CCWGG 2 cut(s) 1146, 1376
BstPI GGTNACC 1 cut(s) 531
BstSCI CCNGG 8 cut(s) 354, 359, 512, 520, 590, 1144, 1374, 1430
BstSLI GKGCMC 2 cut(s) 361, 1437
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 7, 705, 738
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BsuRI GGCC 3 cut(s) 359, 932, 1406
BtgZI GCGATG 1 cut(s) 460
BtrI CACGTC 2 cut(s) 377, 430
BtsCI GGATG 4 cut(s) 126, 257, 769, 1154
BtsI GCAGTG 1 cut(s) 1435
BtsIMutI CAGTG 1 cut(s) 1435
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 399, 481
CfoI GCGC 3 cut(s) 160, 718, 850
Cfr13I GGNCC 3 cut(s) 307, 357, 358
CseI GACGC 2 cut(s) 119, 157
Csp6I GTAC 1 cut(s) 817
CviAII CATG 3 cut(s) 249, 950, 1397
CviQI GTAC 1 cut(s) 817
DdeI CTNAG 7 cut(s) 303, 365, 665, 698, 887, 914, 1264
DpnI GATC 4 cut(s) 13, 72, 568, 596
DpnII GATC 4 cut(s) 11, 70, 566, 594
DraIII CACNNNGTG 1 cut(s) 415
Eam1104I CTCTTC 5 cut(s) 137, 576, 821, 1266, 1461
EarI CTCTTC 5 cut(s) 137, 576, 821, 1266, 1461
Eco130I CCWWGG 1 cut(s) 1407
Eco147I AGGCCT 2 cut(s) 932, 1406
Eco24I GRGCYC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 307
Eco57I CTGAAG 2 cut(s) 557, 1442
Eco72I CACGTG 1 cut(s) 678
Eco91I GGTNACC 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 531
EcoRII CCWGG 2 cut(s) 1144, 1374
EcoT14I CCWWGG 1 cut(s) 1407
EcoT38I GRGCYC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 1407
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 3 cut(s) 252, 953, 1400
FaiI YATR 9 cut(s) 250, 604, 629, 905, 927, 951, 1160, 1178, 1398
FalI AAGNNNNNCTT 4 cut(s) 26, 58, 1298, 1330
FaqI GGGAC 2 cut(s) 496, 1127
FatI CATG 3 cut(s) 248, 949, 1396
Fnu4HI GCNGC 4 cut(s) 18, 21, 719, 752
FokI GGATG 4 cut(s) 113, 244, 776, 1141
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 4 cut(s) 18, 21, 719, 752
FspBI CTAG 3 cut(s) 465, 734, 1446
GlaI GCGC 3 cut(s) 159, 717, 849
GluI GCNGC 4 cut(s) 18, 21, 719, 752
HaeIII GGCC 3 cut(s) 359, 932, 1406
HapII CCGG 8 cut(s) 349, 355, 361, 514, 522, 529, 592, 1431
HgaI GACGC 2 cut(s) 119, 157
HhaI GCGC 3 cut(s) 160, 718, 850
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 3 cut(s) 252, 953, 1400
Hin6I GCGC 3 cut(s) 158, 716, 848
HinP1I GCGC 3 cut(s) 158, 716, 848
HindIII AAGCTT 2 cut(s) 35, 971
HpaII CCGG 8 cut(s) 349, 355, 361, 514, 522, 529, 592, 1431
HphI GGTGA 4 cut(s) 65, 88, 847, 1313
Hpy166II GTNNAC 1 cut(s) 1428
Hpy188III TCNNGA 3 cut(s) 658, 1129, 1165
Hpy8I GTNNAC 1 cut(s) 1428
Hpy99I CGWCG 3 cut(s) 113, 158, 431
HpyAV CCTTC 4 cut(s) 214, 549, 655, 659
HpyCH4III ACNGT 4 cut(s) 154, 183, 386, 743
HpyCH4IV ACGT 3 cut(s) 376, 429, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 757
HpyF3I CTNAG 7 cut(s) 303, 365, 665, 698, 887, 914, 1264
HpySE526I ACGT 3 cut(s) 376, 429, 677
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 3 cut(s) 252, 953, 1400
HspAI GCGC 3 cut(s) 158, 716, 848
Kzo9I GATC 4 cut(s) 11, 70, 566, 594
LmnI GCTCC 1 cut(s) 951
Lsp1109I GCAGC 3 cut(s) 7, 705, 738
LweI GCATC 6 cut(s) 102, 703, 835, 1093, 1163, 1327
MaeI CTAG 3 cut(s) 465, 734, 1446
MaeII ACGT 3 cut(s) 376, 429, 677
MaeIII GTNAC 4 cut(s) 264, 531, 917, 1476
MalI GATC 4 cut(s) 13, 72, 568, 596
MboI GATC 4 cut(s) 11, 70, 566, 594
MfeI CAATTG 1 cut(s) 1036
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 3 cut(s) 361, 426, 1437
MluCI AATT 5 cut(s) 277, 779, 1036, 1063, 1518
MlyI GAGTC 3 cut(s) 296, 479, 647
MmeI TCCRAC 1 cut(s) 315
MroXI GAANNNNTTC 2 cut(s) 743, 1310
MseI TTAA 5 cut(s) 491, 506, 728, 1206, 1326
MspA1I CMGCKG 1 cut(s) 20
MspCI CTTAAG 1 cut(s) 1325
MspI CCGG 8 cut(s) 349, 355, 361, 514, 522, 529, 592, 1431
MspR9I CCNGG 8 cut(s) 356, 361, 514, 522, 592, 1146, 1376, 1432
MunI CAATTG 1 cut(s) 1036
MvaI CCWGG 2 cut(s) 1146, 1376
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 757
NciI CCSGG 6 cut(s) 356, 361, 514, 522, 592, 1432
NdeII GATC 4 cut(s) 11, 70, 566, 594
NlaIII CATG 3 cut(s) 252, 953, 1400
NlaIV GGNNCC 3 cut(s) 353, 359, 710
NmeAIII GCCGAG 1 cut(s) 876
NmuCI GTSAC 2 cut(s) 264, 1476
NspV TTCGAA 1 cut(s) 776
PaqCI CACCTGC 1 cut(s) 409
PceI AGGCCT 2 cut(s) 932, 1406
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PdmI GAANNNNTTC 2 cut(s) 743, 1310
PfeI GAWTC 7 cut(s) 343, 391, 910, 1048, 1192, 1286, 1339
PfoI TCCNGGA 2 cut(s) 512, 590
PkrI GCNGC 4 cut(s) 19, 22, 720, 753
PleI GAGTC 3 cut(s) 295, 479, 646
PmaCI CACGTG 1 cut(s) 678
PmlI CACGTG 1 cut(s) 678
PpsI GAGTC 3 cut(s) 295, 479, 646
Ppu21I YACGTR 1 cut(s) 678
PpuMI RGGWCCY 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 2 cut(s) 1144, 1374
PspCI CACGTG 1 cut(s) 678
PspEI GGTNACC 1 cut(s) 531
PspGI CCWGG 2 cut(s) 1144, 1374
PspN4I GGNNCC 3 cut(s) 353, 359, 710
PspOMI GGGCCC 1 cut(s) 357
PspPI GGNCC 3 cut(s) 307, 357, 358
PspPPI RGGWCCY 1 cut(s) 307
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 1 cut(s) 818
RsaNI GTAC 1 cut(s) 817
SaqAI TTAA 5 cut(s) 491, 506, 728, 1206, 1326
SatI GCNGC 4 cut(s) 18, 21, 719, 752
Sau3AI GATC 4 cut(s) 11, 70, 566, 594
Sau96I GGNCC 3 cut(s) 307, 357, 358
SchI GAGTC 3 cut(s) 296, 479, 647
ScrFI CCNGG 8 cut(s) 356, 361, 514, 522, 592, 1146, 1376, 1432
SduI GDGCHC 3 cut(s) 361, 426, 1437
SfaNI GCATC 6 cut(s) 102, 703, 835, 1093, 1163, 1327
SfuI TTCGAA 1 cut(s) 776
SinI GGWCC 1 cut(s) 307
SmlI CTYRAG 2 cut(s) 656, 1325
SmoI CTYRAG 2 cut(s) 656, 1325
Sse9I AATT 5 cut(s) 277, 779, 1036, 1063, 1518
SseBI AGGCCT 2 cut(s) 932, 1406
SsiI CCGC 4 cut(s) 18, 91, 558, 793
SspI AATATT 1 cut(s) 85
SspMI CTAG 3 cut(s) 465, 734, 1446
StuI AGGCCT 2 cut(s) 932, 1406
StyD4I CCNGG 8 cut(s) 354, 359, 512, 520, 590, 1144, 1374, 1430
StyI CCWWGG 1 cut(s) 1407
TaaI ACNGT 4 cut(s) 154, 183, 386, 743
TaiI ACGT 3 cut(s) 379, 432, 680
TaqI TCGA 6 cut(s) 216, 426, 672, 747, 776, 1029
TasI AATT 5 cut(s) 277, 779, 1036, 1063, 1518
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 7 cut(s) 343, 391, 910, 1048, 1192, 1286, 1339
Tru1I TTAA 5 cut(s) 491, 506, 728, 1206, 1326
Tru9I TTAA 5 cut(s) 491, 506, 728, 1206, 1326
TscAI CASTG 1 cut(s) 1442
TseFI GTSAC 2 cut(s) 264, 1476
TseI GCWGC 3 cut(s) 20, 718, 751
Tsp45I GTSAC 2 cut(s) 264, 1476
TspDTI ATGAA 3 cut(s) 72, 966, 1380
TspRI CASTG 1 cut(s) 1442
Vha464I CTTAAG 1 cut(s) 1325
VpaK11BI GGWCC 1 cut(s) 307
XmnI GAANNNNTTC 2 cut(s) 743, 1310
XspI CTAG 3 cut(s) 465, 734, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.