MD17G1103300.v1.1
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007368
Physical Location & Seq
Forward (+)
8775534 .. 8779421
3888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1103300.v1.1.491

Sequence Viewer

Length: 1437 bp
ATGAACTTCGTCTCACTCTCTGCTTACCTTGATCCGATGTGGTTGGCTACTACCGTACATCACCAGCCGGTCCGTCCGGTTCCGACGATCTCCACGGCGGGTCCGAACCCGAAAGTCGTGTATCCCTGTCTTCCGAAACCGAAACCGTTTCGGTTCGGACCCGTCGGTGTCCGGCTCCGCCTTAACAAGTACAAAGTTTTGGCGGAAAAGAAGCAGCTCGCCGATTTAGGGACTCAGAATTCAGATAGTGAGAAATTAGATGTAGGAGAAGAGAAAGTTGTAGAAGAAGAGGAAGTTGAAGGTTTGGATTTGGGATGGTTAGCTGCTTTTCCGCACGTGTTTATAGCTTCCATGTCGAATTTTCTGTTCGGTTATCACATTGGGGTGATGAATGGTCCGATTGTTTCGATTGCCCGAGAACTCGGTTTCGAAGGGAATTCGATTCTGGAGGGACTTGTAGTTAGCATTTTCATTGTTGGTGCATTTCTTGGAAGCCTATGCTGCGGCACAATTGTTGATAAACTTGGTTGTAGAAGGACTTTTCAGATTGCCACTATTCCTCTCATTCTTGGTGCATTGATAAGTGCACAAGCTCATTCCTTGGATGAAGTGACTTTTGGAAGGTTTCTTGTTGGCCTTGGCATTGGTGTTGACACTGTTCTTGTACCAATCTATATCTCAGAGGTAGGGAGACTGGACAATGCTAAAGAAGTTATCAAAAATGTTTGGGGAGCATCTGAAGTTGAAAAGGCGATTGAAGAATTTCAGTCAGTTATCAAGAATGATGGTAGTGATTTGGACAGCAGCTGGTTTGAACTTTTGAAGGAGCCAAACTCAAGAGGTTGTATCAAGTTTGCAGGCATAAACGGAGTTCTTTACTTTTCATCATTGACTTTCAAAGATGTTGGAATCACAAGTAGCGCTTTGGCAAGTTTGTTGATCGGAGTTACGAACTTTGCAGGGGCACTTTGTGCTTTGTACTTGATTGATAAGCAGGGAAGGCAGAAGCTCTTGATTGGAAGTTACTTGGGAATGGCACTTTCGATGTTTCTTGTGGTCTATGCCATCAGTTTTCCCTTAGATGAGCAACTAAGTCACAACTTATCGATACTTGGAACAGTCATGTACATATTCACCTTTGCAATAGGAGCTGGCCCAGTGACAGGCCTTATCATACCAGAACTTAGCAGCAACAGGTCACGCGGGAAGATCATGGGATTCAGCTTTTCTGCGCATTGGGTTTGCAACTTTTTGGTGGGTCTGTTTTTCCTCGACTTAGTGGAGAAACTCGGCGTTGCTCCAGTTTATGGTACCTTCGGTGGAGTCTCCTTGTTGACAGCAATTTTTGCAAACTACTTTATCGTTGAAACTAAAGGCCGCTCCCTTGAGGAAATAGAAATGTCACTAACCTCCGATTTCAGGGCTAGAGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

479

Amino Acids

51.96

Weight (kDa)

5.63

Isoelectric Point (pI)

27.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 113 - 229 4.1e-23 Sugar (and other) transporter
MFS_1 PF07690 116 - 228 2.7e-08 Major Facilitator Superfamily
Sugar_tr PF00083 229 - 466 4.5e-47 Sugar (and other) transporter
MFS_1 PF07690 299 - 460 7.9e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1233
Acc65I GGTACC 1 cut(s) 1310
AccB1I GGYRCC 1 cut(s) 1310
AccB7I CCANNNNNTGG 1 cut(s) 1307
AccBSI CCGCTC 1 cut(s) 1380
AccII CGCG 1 cut(s) 1203
AciI CCGC 7 cut(s) 98, 178, 203, 332, 504, 1203, 1378
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 4 cut(s) 238, 358, 436, 761
AcuI CTGAAG 1 cut(s) 759
AcvI CACGTG 1 cut(s) 337
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 6 cut(s) 57, 191, 666, 980, 1127, 1312
AfeI AGCGCT 1 cut(s) 922
AfiI CCNNNNNNNGG 4 cut(s) 228, 1163, 1307, 1419
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 7 cut(s) 299, 746, 758, 815, 823, 898, 1367
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
AluBI AGCT 8 cut(s) 217, 323, 347, 593, 807, 1009, 1151, 1224
AluI AGCT 8 cut(s) 217, 323, 347, 593, 807, 1009, 1151, 1224
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 4 cut(s) 16, 685, 1330, 1422
Alw44I GTGCAC 1 cut(s) 585
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 807
Ama87I CYCGRG 1 cut(s) 414
Aor51HI AGCGCT 1 cut(s) 922
AoxI GGCC 4 cut(s) 634, 1153, 1165, 1375
ApaLI GTGCAC 1 cut(s) 585
ApeKI GCWGC 5 cut(s) 214, 323, 501, 804, 1188
ApoI RAATTY 4 cut(s) 238, 358, 436, 761
Asp700I GAANNNNTTC 1 cut(s) 762
Asp718I GGTACC 1 cut(s) 1310
AspLEI GCGC 2 cut(s) 923, 1234
AspS9I GGNCC 5 cut(s) 70, 101, 158, 395, 1154
AsuHPI GGTGA 3 cut(s) 53, 397, 1126
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 4 cut(s) 70, 101, 158, 395
BaeGI GKGCMC 2 cut(s) 589, 967
BanI GGYRCC 1 cut(s) 1310
BbrPI CACGTG 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 589
BbvI GCAGC 5 cut(s) 226, 310, 488, 816, 1200
BccI CCATC 3 cut(s) 309, 779, 1073
BceAI ACGGC 1 cut(s) 111
BciVI GTATCC 1 cut(s) 132
BcoDI GTCTC 4 cut(s) 16, 685, 1330, 1422
BfaI CTAG 1 cut(s) 1425
BfoI RGCGCY 1 cut(s) 924
BfuI GTATCC 1 cut(s) 132
BisI GCNGC 7 cut(s) 215, 324, 502, 505, 805, 1189, 1378
BlsI GCNGC 7 cut(s) 216, 325, 503, 506, 806, 1190, 1379
Bme18I GGWCC 4 cut(s) 70, 101, 158, 395
BmeT110I CYCGRG 1 cut(s) 414
BmgT120I GGNCC 5 cut(s) 70, 101, 158, 395, 1154
BmiI GGNNCC 6 cut(s) 81, 102, 160, 176, 828, 1312
BmrI ACTGGG 1 cut(s) 1151
BmsI GCATC 1 cut(s) 743
BmuI ACTGGG 1 cut(s) 1151
BpiI GAAGAC 1 cut(s) 122
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 2 cut(s) 467, 1284
Bpu14I TTCGAA 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 820, 1406
Bsa29I ATCGAT 1 cut(s) 1106
BsaAI YACGTR 1 cut(s) 337
BsaJI CCNNGG 3 cut(s) 93, 600, 637
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 1163, 1307, 1419
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 699, 1157, 1301
BseCI ATCGAT 1 cut(s) 1106
BseDI CCNNGG 3 cut(s) 93, 600, 637
BseGI GGATG 2 cut(s) 320, 610
BseLI CCNNNNNNNGG 4 cut(s) 228, 1163, 1307, 1419
BseMII CTCAG 2 cut(s) 248, 693
BseNI ACTGG 3 cut(s) 699, 1157, 1301
BseSI GKGCMC 2 cut(s) 589, 967
BseXI GCAGC 5 cut(s) 226, 310, 488, 816, 1200
Bsh1236I CGCG 1 cut(s) 1203
BshFI GGCC 4 cut(s) 636, 1155, 1167, 1377
BshNI GGYRCC 1 cut(s) 1310
BshVI ATCGAT 1 cut(s) 1106
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 3 cut(s) 68, 77, 172
BslFI GGGAC 2 cut(s) 244, 465
BslI CCNNNNNNNGG 4 cut(s) 228, 1163, 1307, 1419
BsmAI GTCTC 4 cut(s) 16, 685, 1330, 1422
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 2 cut(s) 244, 465
BsnI GGCC 4 cut(s) 636, 1155, 1167, 1377
BsoBI CYCGRG 1 cut(s) 414
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 589, 967
Bsp1407I TGTACA 1 cut(s) 1125
Bsp143I GATC 4 cut(s) 31, 87, 939, 1209
BspACI CCGC 7 cut(s) 98, 178, 203, 332, 504, 1203, 1378
BspANI GGCC 4 cut(s) 636, 1155, 1167, 1377
BspCNI CTCAG 2 cut(s) 247, 692
BspDI ATCGAT 1 cut(s) 1106
BspFNI CGCG 1 cut(s) 1203
BspLI GGNNCC 6 cut(s) 81, 102, 160, 176, 828, 1312
BspPI GGATC 1 cut(s) 26
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 1310
BsrBI CCGCTC 1 cut(s) 1380
BsrFI RCCGGY 1 cut(s) 67
BsrGI TGTACA 1 cut(s) 1125
BsrI ACTGG 3 cut(s) 699, 1157, 1301
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 3 cut(s) 93, 600, 637
BssMI GATC 4 cut(s) 31, 87, 939, 1209
BssT1I CCWWGG 2 cut(s) 600, 637
Bst4CI ACNGT 4 cut(s) 55, 147, 658, 1120
Bst6I CTCTTC 2 cut(s) 264, 282
BstAPI GCANNNNNTGC 2 cut(s) 971, 1346
BstAUI TGTACA 1 cut(s) 1125
BstBAI YACGTR 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 219, 859, 1153
BstDEI CTNAG 6 cut(s) 234, 679, 1078, 1091, 1184, 1276
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 2 cut(s) 320, 610
BstFNI CGCG 1 cut(s) 1203
BstH2I RGCGCY 1 cut(s) 924
BstHHI GCGC 2 cut(s) 923, 1234
BstKTI GATC 4 cut(s) 34, 90, 942, 1212
BstMAI GTCTC 4 cut(s) 16, 685, 1330, 1422
BstMBI GATC 4 cut(s) 31, 87, 939, 1209
BstMWI GCNNNNNNNGC 5 cut(s) 501, 971, 1000, 1148, 1346
BstSLI GKGCMC 2 cut(s) 589, 967
BstUI CGCG 1 cut(s) 1203
BstV1I GCAGC 5 cut(s) 226, 310, 488, 816, 1200
BstV2I GAAGAC 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 1106
BsuI GTATCC 1 cut(s) 132
BsuRI GGCC 4 cut(s) 636, 1155, 1167, 1377
BsuTUI ATCGAT 1 cut(s) 1106
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 2 cut(s) 320, 610
BtsIMutI CAGTG 2 cut(s) 654, 1164
Cac8I GCNNGC 3 cut(s) 219, 859, 1153
CaiI CAGNNNCTG 1 cut(s) 807
CfoI GCGC 2 cut(s) 923, 1234
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 5 cut(s) 70, 101, 158, 395, 1154
ClaI ATCGAT 1 cut(s) 1106
CpoI CGGWCCG 1 cut(s) 70
Csp6I GTAC 6 cut(s) 56, 190, 665, 979, 1126, 1311
CspI CGGWCCG 1 cut(s) 70
CviAII CATG 3 cut(s) 352, 1123, 1213
CviQI GTAC 6 cut(s) 56, 190, 665, 979, 1126, 1311
DdeI CTNAG 6 cut(s) 234, 679, 1078, 1091, 1184, 1276
DpnI GATC 4 cut(s) 33, 89, 941, 1211
DpnII GATC 4 cut(s) 31, 87, 939, 1209
DraIII CACNNNGTG 1 cut(s) 971
Eam1104I CTCTTC 2 cut(s) 264, 282
EarI CTCTTC 2 cut(s) 264, 282
EciI GGCGGA 2 cut(s) 167, 218
Eco130I CCWWGG 2 cut(s) 600, 637
Eco147I AGGCCT 1 cut(s) 1167
Eco47I GGWCC 4 cut(s) 70, 101, 158, 395
Eco47III AGCGCT 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 759
Eco72I CACGTG 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 414
EcoRI GAATTC 2 cut(s) 238, 436
EcoT14I CCWWGG 2 cut(s) 600, 637
ErhI CCWWGG 2 cut(s) 600, 637
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 3 cut(s) 355, 1126, 1216
FalI AAGNNNNNCTT 2 cut(s) 907, 939
FaqI GGGAC 2 cut(s) 244, 465
FatI CATG 3 cut(s) 351, 1122, 1212
FauI CCCGC 2 cut(s) 91, 1196
Fnu4HI GCNGC 7 cut(s) 215, 324, 502, 505, 805, 1189, 1378
FokI GGATG 2 cut(s) 327, 617
Fsp4HI GCNGC 7 cut(s) 215, 324, 502, 505, 805, 1189, 1378
FspBI CTAG 1 cut(s) 1425
FspI TGCGCA 1 cut(s) 1233
GlaI GCGC 2 cut(s) 922, 1233
GluI GCNGC 7 cut(s) 215, 324, 502, 505, 805, 1189, 1378
GsuI CTGGAG 2 cut(s) 467, 1284
HaeII RGCGCY 1 cut(s) 924
HaeIII GGCC 4 cut(s) 636, 1155, 1167, 1377
HapII CCGG 3 cut(s) 68, 77, 172
HhaI GCGC 2 cut(s) 923, 1234
Hin1II CATG 3 cut(s) 355, 1126, 1216
Hin6I GCGC 2 cut(s) 921, 1232
HinP1I GCGC 2 cut(s) 921, 1232
HincII GTYRAC 2 cut(s) 652, 1335
HindII GTYRAC 2 cut(s) 652, 1335
HinfI GANTC 5 cut(s) 232, 442, 909, 1218, 1323
HpaII CCGG 3 cut(s) 68, 77, 172
HphI GGTGA 3 cut(s) 53, 397, 1126
Hpy166II GTNNAC 3 cut(s) 587, 652, 1335
Hpy188III TCNNGA 4 cut(s) 446, 778, 837, 1012
Hpy8I GTNNAC 3 cut(s) 587, 652, 1335
Hpy99I CGWCG 2 cut(s) 88, 167
HpyAV CCTTC 7 cut(s) 293, 425, 528, 615, 817, 993, 1324
HpyCH4III ACNGT 4 cut(s) 55, 147, 658, 1120
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 8 cut(s) 482, 575, 587, 857, 959, 1142, 1245, 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 501, 971, 1000, 1148, 1346
HpyF3I CTNAG 6 cut(s) 234, 679, 1078, 1091, 1184, 1276
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 3 cut(s) 355, 1126, 1216
HspAI GCGC 2 cut(s) 921, 1232
KpnI GGTACC 1 cut(s) 1314
Kzo9I GATC 4 cut(s) 31, 87, 939, 1209
LmnI GCTCC 6 cut(s) 180, 731, 826, 1148, 1303, 1385
Lsp1109I GCAGC 5 cut(s) 226, 310, 488, 816, 1200
LweI GCATC 1 cut(s) 743
MaeI CTAG 1 cut(s) 1425
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 7 cut(s) 610, 946, 1022, 1094, 1159, 1197, 1401
MalI GATC 4 cut(s) 33, 89, 941, 1211
MbiI CCGCTC 1 cut(s) 1380
MboI GATC 4 cut(s) 31, 87, 939, 1209
MboII GAAGA 6 cut(s) 122, 281, 296, 299, 770, 1219
MfeI CAATTG 1 cut(s) 510
MhlI GDGCHC 2 cut(s) 589, 967
MluCI AATT 7 cut(s) 238, 254, 358, 436, 510, 761, 1341
MlyI GAGTC 2 cut(s) 226, 1332
MmeI TCCRAC 2 cut(s) 107, 886
MnlI CCTC 8 cut(s) 283, 442, 570, 676, 833, 1280, 1381, 1420
MroXI GAANNNNTTC 1 cut(s) 762
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 807
MspI CCGG 3 cut(s) 68, 77, 172
MunI CAATTG 1 cut(s) 510
MvnI CGCG 1 cut(s) 1203
MwoI GCNNNNNNNGC 5 cut(s) 501, 971, 1000, 1148, 1346
NdeII GATC 4 cut(s) 31, 87, 939, 1209
NlaIII CATG 3 cut(s) 355, 1126, 1216
NlaIV GGNNCC 6 cut(s) 81, 102, 160, 176, 828, 1312
NmeAIII GCCGAG 1 cut(s) 1269
NmuCI GTSAC 5 cut(s) 610, 1094, 1159, 1197, 1401
NsbI TGCGCA 1 cut(s) 1233
NspV TTCGAA 1 cut(s) 429
PceI AGGCCT 1 cut(s) 1167
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 3 cut(s) 442, 909, 1218
PflMI CCANNNNNTGG 1 cut(s) 1307
PkrI GCNGC 7 cut(s) 216, 325, 503, 506, 806, 1190, 1379
PleI GAGTC 2 cut(s) 226, 1331
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
PpsI GAGTC 2 cut(s) 226, 1331
Ppu21I YACGTR 1 cut(s) 337
PspCI CACGTG 1 cut(s) 337
PspN4I GGNNCC 6 cut(s) 81, 102, 160, 176, 828, 1312
PspPI GGNCC 5 cut(s) 70, 101, 158, 395, 1154
PstNI CAGNNNCTG 1 cut(s) 807
PvuII CAGCTG 1 cut(s) 807
RsaI GTAC 6 cut(s) 57, 191, 666, 980, 1127, 1312
RsaNI GTAC 6 cut(s) 56, 190, 665, 979, 1126, 1311
RseI CAYNNNNRTG 1 cut(s) 383
Rsr2I CGGWCCG 1 cut(s) 70
RsrII CGGWCCG 1 cut(s) 70
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 7 cut(s) 215, 324, 502, 505, 805, 1189, 1378
Sau3AI GATC 4 cut(s) 31, 87, 939, 1209
Sau96I GGNCC 5 cut(s) 70, 101, 158, 395, 1154
SchI GAGTC 2 cut(s) 226, 1332
SduI GDGCHC 2 cut(s) 589, 967
SfaNI GCATC 1 cut(s) 743
SfuI TTCGAA 1 cut(s) 429
SinI GGWCC 4 cut(s) 70, 101, 158, 395
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 835, 1385
SmoI CTYRAG 2 cut(s) 835, 1385
Sse9I AATT 7 cut(s) 238, 254, 358, 436, 510, 761, 1341
SseBI AGGCCT 1 cut(s) 1167
SsiI CCGC 7 cut(s) 98, 178, 203, 332, 504, 1203, 1378
SspMI CTAG 1 cut(s) 1425
StuI AGGCCT 1 cut(s) 1167
StyI CCWWGG 2 cut(s) 600, 637
TaaI ACNGT 4 cut(s) 55, 147, 658, 1120
TaiI ACGT 1 cut(s) 339
TaqI TCGA 7 cut(s) 356, 407, 429, 440, 1043, 1106, 1272
TasI AATT 7 cut(s) 238, 254, 358, 436, 510, 761, 1341
TatI WGTACW 3 cut(s) 189, 978, 1125
TauI GCSGC 2 cut(s) 507, 1380
TfiI GAWTC 3 cut(s) 442, 909, 1218
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 2 cut(s) 661, 1164
TseFI GTSAC 5 cut(s) 610, 1094, 1159, 1197, 1401
TseI GCWGC 5 cut(s) 214, 323, 501, 804, 1188
Tsp45I GTSAC 5 cut(s) 610, 1094, 1159, 1197, 1401
TspDTI ATGAA 5 cut(s) 17, 404, 460, 621, 873
TspGWI ACGGA 2 cut(s) 62, 882
TspRI CASTG 2 cut(s) 661, 1164
Van91I CCANNNNNTGG 1 cut(s) 1307
VneI GTGCAC 1 cut(s) 585
VpaK11BI GGWCC 4 cut(s) 70, 101, 158, 395
XapI RAATTY 4 cut(s) 238, 358, 436, 761
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 1 cut(s) 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.