MD17G1106300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015010
Physical Location & Seq
Forward (+)
9066736 .. 9068460
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1106300.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGAGAACTTCCCAGTTATCAACTTGGAGAGCCTCAATGGTGAGGGAAGAAAAGCTACAATGGAGAAAATCAAAGATGCCTGTGAGAACTGGGGTTTCTTTGAGCTGGTGAGTCATGGGATTCCAACTGAGTTTTTGGACACAGTGGAGAGGCTGACAAAAGAGCACTACCGGCAGTGTATGGAGCAAAGGTTCAAGGAGTTGGTGGCCAGCAAAGGCCTTGATGCCGTTCAGACAGAAGTCAACGATATGGATTGGGAAAGCACTTTCTACTTGCGCCATCTTCCTCAATCCAACATCTCTGAAGTCCCAGATCTCAAGGATGAGTACAGGAATGTGATGAAGGAGTTTGCATTGAAACTGGAGAAATTAGCAGAGCAGCTGCTGGGCTTGTTGTGTGAGAATCTTGGACTGGAACAAGGGTACCTTACGAAGGCATTTTATGGAACAAATGGACCAACTTTTGGCACCAAGGTTAGCAACTACCCTCCATGTCCCAACAATGACAAGATCAAGGGTCTCCGGGCCCACACCGATGCCGGCGGCCTCATCTTGCTCTTCCAGGATGACAAGGTCAGTGGCCTCCAGCTCCTCAAGGACGGAGAGTGGGTTGATGTGCCTCCCATGCGCCACTCCCTTGTTATCAACCTTGGTGACCAACTTGAGGTGATCACCAATGGAAAGTACAAGAGTGTGGAGCACAGAGTGATTGCCCAAACAGATGGAACCAGAATGTCAATAGCTTCATTCTACAACCCAAGCAGTGATGCAGTGATCTACCCAGCACCAACCCTAGTGGAGAAAAAGGCAGTGGAGAAGAATCAAGTGTACCCGAAATTCGTGTTTGAAGACTACATGAAGCACTATGTTGGTGTCAAGTTCCAGGCCAAGGAACCCAGATTTGAAGCTATGAAAGCAGTGGAAGTTAAGGCCAGTTTTGGTTTGAGTCCAGTTAAAACTACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009506 GO:0009593 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009692 GO:0009693 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009735 GO:0009815 GO:0009987 GO:0010029 GO:0010030 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010817 GO:0012505 GO:0016020 GO:0016491 GO:0016638 GO:0016641 GO:0030054 GO:0030312 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043449 GO:0043450 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0048518 GO:0048580 GO:0048582 GO:0050789 GO:0050793 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0055114 GO:0065007 GO:0065008 GO:0070542 GO:0070887 GO:0071229 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071396 GO:0071398 GO:0071704 GO:0071731 GO:0071732 GO:0071944 GO:0097366 GO:1900140 GO:1900673 GO:1900674 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko01110 map00270 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

323

Amino Acids

36.47

Weight (kDa)

5.63

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 5 - 98 8.9e-15 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 159 - 253 1.7e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05010
fragaria_vesca FvH4_6g42090
malus_domestica MD09G1114800.v1.1 MD17G1106300.v1.1
prunus_persica Prupe.3G209900_v2.0.a1
pyrus_communis pycom17g09900 pycom17g09920
rosa_chinensis RchiOBHm_Chr2g0158011
rosa_laevigata RLG00000021048
rosa_multiflora Rmu_sc0000419.1_g000014
rosa_roxburghii Rroxscaffold_2G00091750
rosa_rugosa Rorug02G0465200
rosa_samantha Rh2AG531100 Rh2CG515400 Rh2DG553700
rosa_wichuraiana Rw2G043920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 423, 467
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 1 cut(s) 543
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 836
AcuI CTGAAG 1 cut(s) 324
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 4 cut(s) 329, 425, 686, 830
AfiI CCNNNNNNNGG 4 cut(s) 433, 464, 664, 889
AgsI TTSAA 4 cut(s) 196, 358, 848, 905
AjnI CCWGG 2 cut(s) 561, 882
AloI GAACNNNNNNTCC 2 cut(s) 176, 208
AluBI AGCT 6 cut(s) 56, 106, 382, 589, 743, 908
AluI AGCT 6 cut(s) 56, 106, 382, 589, 743, 908
Alw21I GWGCWC 2 cut(s) 168, 702
Alw26I GTCTC 1 cut(s) 524
AlwNI CAGNNNCTG 1 cut(s) 385
AoxI GGCC 7 cut(s) 207, 217, 525, 544, 580, 885, 930
ApaI GGGCCC 1 cut(s) 529
ApeKI GCWGC 2 cut(s) 379, 382
ApoI RAATTY 1 cut(s) 836
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 2 cut(s) 279, 630
AspS9I GGNCC 3 cut(s) 455, 525, 526
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 5 cut(s) 53, 121, 664, 665, 679
AvaII GGWCC 1 cut(s) 455
BaeGI GKGCMC 1 cut(s) 529
BaeI ACNNNNGTAYC 2 cut(s) 407, 440
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 2 cut(s) 423, 467
BanII GRGCYC 1 cut(s) 529
BarI GAAGNNNNNNTAC 2 cut(s) 40, 72
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 168, 702
BbvI GCAGC 2 cut(s) 369, 391
BccI CCATC 2 cut(s) 288, 716
BceAI ACGGC 1 cut(s) 212
BciT130I CCWGG 2 cut(s) 563, 884
BclI TGATCA 1 cut(s) 669
BcnI CCSGG 1 cut(s) 524
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 1 cut(s) 794
BglII AGATCT 1 cut(s) 313
BisI GCNGC 3 cut(s) 380, 383, 544
BlsI GCNGC 3 cut(s) 381, 384, 545
Bme1390I CCNGG 3 cut(s) 524, 563, 884
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 3 cut(s) 455, 525, 526
BmiI GGNNCC 5 cut(s) 425, 469, 527, 727, 894
BmrFI CCNGG 3 cut(s) 524, 563, 884
BmrI ACTGGG 2 cut(s) 8, 100
BmsI GCATC 4 cut(s) 67, 214, 526, 757
BmuI ACTGGG 2 cut(s) 8, 100
BoxI GACNNNNGTC 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 855
BpmI CTGGAG 2 cut(s) 383, 569
BpuEI CTTGAG 3 cut(s) 302, 578, 683
BpuMI CCSGG 1 cut(s) 524
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 3 cut(s) 471, 649, 888
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 433, 464, 664, 889
Bse118I RCCGGY 2 cut(s) 171, 539
Bse1I ACTGG 6 cut(s) 14, 95, 366, 417, 933, 950
BseBI CCWGG 2 cut(s) 563, 884
BseDI CCNNGG 3 cut(s) 471, 649, 888
BseGI GGATG 2 cut(s) 328, 571
BseLI CCNNNNNNNGG 4 cut(s) 433, 464, 664, 889
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 6 cut(s) 14, 95, 366, 417, 933, 950
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 2 cut(s) 369, 391
BseYI CCCAGC 2 cut(s) 385, 781
BshFI GGCC 7 cut(s) 209, 219, 527, 546, 582, 887, 932
BshNI GGYRCC 2 cut(s) 423, 467
BsiHKAI GWGCWC 2 cut(s) 168, 702
BsiSI CCGG 3 cut(s) 172, 523, 540
BslFI GGGAC 2 cut(s) 293, 480
BslI CCNNNNNNNGG 4 cut(s) 433, 464, 664, 889
BsmAI GTCTC 1 cut(s) 524
BsmFI GGGAC 2 cut(s) 293, 480
BsnI GGCC 7 cut(s) 209, 219, 527, 546, 582, 887, 932
Bso31I GGTCTC 1 cut(s) 524
Bsp120I GGGCCC 1 cut(s) 525
Bsp1286I GDGCHC 3 cut(s) 168, 529, 702
Bsp143I GATC 4 cut(s) 313, 510, 669, 774
BspACI CCGC 1 cut(s) 543
BspANI GGCC 7 cut(s) 209, 219, 527, 546, 582, 887, 932
BspCNI CTCAG 1 cut(s) 121
BspLI GGNNCC 5 cut(s) 425, 469, 527, 727, 894
BspQI GCTCTTC 1 cut(s) 563
BspT107I GGYRCC 2 cut(s) 423, 467
BspTNI GGTCTC 1 cut(s) 524
BsrFI RCCGGY 2 cut(s) 171, 539
BsrI ACTGG 6 cut(s) 14, 95, 366, 417, 933, 950
BssAI RCCGGY 2 cut(s) 171, 539
BssECI CCNNGG 3 cut(s) 471, 649, 888
BssMI GATC 4 cut(s) 313, 510, 669, 774
BssT1I CCWWGG 3 cut(s) 471, 649, 888
Bst2UI CCWGG 2 cut(s) 563, 884
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 563
BstC8I GCNNGC 2 cut(s) 211, 541
BstDEI CTNAG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 653
BstENI CCTNNNNNAGG 1 cut(s) 431
BstF5I GGATG 2 cut(s) 328, 571
BstHHI GCGC 2 cut(s) 279, 630
BstKTI GATC 4 cut(s) 316, 513, 672, 777
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 4 cut(s) 313, 510, 669, 774
BstMWI GCNNNNNNNGC 3 cut(s) 172, 625, 914
BstNI CCWGG 2 cut(s) 563, 884
BstPAI GACNNNNGTC 1 cut(s) 239
BstPI GGTNACC 1 cut(s) 653
BstSCI CCNGG 3 cut(s) 522, 561, 882
BstSLI GKGCMC 1 cut(s) 529
BstV1I GCAGC 2 cut(s) 369, 391
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 313
BstXI CCANNNNNNTGG 1 cut(s) 722
BstYI RGATCY 1 cut(s) 313
BsuRI GGCC 7 cut(s) 209, 219, 527, 546, 582, 887, 932
BtsCI GGATG 2 cut(s) 328, 571
BtsI GCAGTG 5 cut(s) 182, 769, 777, 816, 924
BtsIMutI CAGTG 7 cut(s) 150, 182, 583, 769, 777, 816, 924
Cac8I GCNNGC 2 cut(s) 211, 541
CaiI CAGNNNCTG 1 cut(s) 385
CfoI GCGC 2 cut(s) 279, 630
Cfr10I RCCGGY 2 cut(s) 171, 539
Cfr13I GGNCC 3 cut(s) 455, 525, 526
Csp6I GTAC 4 cut(s) 328, 424, 685, 829
CspCI CAANNNNNGTGG 4 cut(s) 559, 594, 777, 812
CviAII CATG 4 cut(s) 116, 492, 625, 856
CviQI GTAC 4 cut(s) 328, 424, 685, 829
DdeI CTNAG 1 cut(s) 129
DpnI GATC 4 cut(s) 315, 512, 671, 776
DpnII GATC 4 cut(s) 313, 510, 669, 774
DraIII CACNNNGTG 1 cut(s) 706
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco130I CCWWGG 3 cut(s) 471, 649, 888
Eco147I AGGCCT 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 529
Eco31I GGTCTC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 324
Eco91I GGTNACC 1 cut(s) 653
EcoNI CCTNNNNNAGG 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 561, 882
EcoT14I CCWWGG 3 cut(s) 471, 649, 888
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 3 cut(s) 471, 649, 888
FaeI CATG 4 cut(s) 119, 495, 628, 859
FaiI YATR 9 cut(s) 117, 182, 251, 444, 493, 626, 857, 867, 911
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 2 cut(s) 293, 480
FatI CATG 4 cut(s) 115, 491, 624, 855
FbaI TGATCA 1 cut(s) 669
Fnu4HI GCNGC 3 cut(s) 380, 383, 544
FokI GGATG 2 cut(s) 335, 578
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 3 cut(s) 380, 383, 544
FspBI CTAG 1 cut(s) 794
GlaI GCGC 2 cut(s) 278, 629
GluI GCNGC 3 cut(s) 380, 383, 544
GsaI CCCAGC 2 cut(s) 389, 785
GsuI CTGGAG 2 cut(s) 383, 569
HaeIII GGCC 7 cut(s) 209, 219, 527, 546, 582, 887, 932
HapII CCGG 3 cut(s) 172, 523, 540
HhaI GCGC 2 cut(s) 279, 630
Hin1II CATG 4 cut(s) 119, 495, 628, 859
Hin6I GCGC 2 cut(s) 277, 628
HinP1I GCGC 2 cut(s) 277, 628
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 5 cut(s) 112, 121, 403, 820, 946
HpaII CCGG 3 cut(s) 172, 523, 540
HphI GGTGA 5 cut(s) 53, 121, 664, 665, 679
Hpy166II GTNNAC 2 cut(s) 244, 829
Hpy188I TCNGA 2 cut(s) 234, 304
Hpy8I GTNNAC 2 cut(s) 244, 829
HpyAV CCTTC 2 cut(s) 337, 427
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4V TGCA 2 cut(s) 353, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 625, 914
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 4 cut(s) 119, 495, 628, 859
HspAI GCGC 2 cut(s) 277, 628
KpnI GGTACC 1 cut(s) 427
KroI GCCGGC 1 cut(s) 539
KroNI GCCGGC 1 cut(s) 541
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 4 cut(s) 313, 510, 669, 774
LguI GCTCTTC 1 cut(s) 563
LmnI GCTCC 3 cut(s) 184, 594, 697
Lsp1109I GCAGC 2 cut(s) 369, 391
LweI GCATC 4 cut(s) 67, 214, 526, 757
MaeI CTAG 1 cut(s) 794
MaeIII GTNAC 1 cut(s) 653
MalI GATC 4 cut(s) 315, 512, 671, 776
MboI GATC 4 cut(s) 313, 510, 669, 774
MboII GAAGA 5 cut(s) 60, 275, 550, 829, 860
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 3 cut(s) 168, 529, 702
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 2 cut(s) 368, 836
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 121, 955
MmeI TCCRAC 2 cut(s) 149, 318
Mox20I TGGCCA 1 cut(s) 209
MroNI GCCGGC 1 cut(s) 539
MscI TGGCCA 1 cut(s) 209
MseI TTAA 2 cut(s) 927, 954
MslI CAYNNNNRTG 1 cut(s) 534
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 3 cut(s) 172, 523, 540
MspR9I CCNGG 3 cut(s) 524, 563, 884
MvaI CCWGG 2 cut(s) 563, 884
MwoI GCNNNNNNNGC 3 cut(s) 172, 625, 914
NaeI GCCGGC 1 cut(s) 541
NciI CCSGG 1 cut(s) 524
NdeII GATC 4 cut(s) 313, 510, 669, 774
NgoMIV GCCGGC 1 cut(s) 539
NlaIII CATG 4 cut(s) 119, 495, 628, 859
NlaIV GGNNCC 5 cut(s) 425, 469, 527, 727, 894
NmuCI GTSAC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 563
PdiI GCCGGC 1 cut(s) 541
PfeI GAWTC 3 cut(s) 121, 403, 820
PflFI GACNNNGTC 1 cut(s) 572
PflMI CCANNNNNTGG 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 3 cut(s) 381, 384, 545
PleI GAGTC 2 cut(s) 120, 954
PpsI GAGTC 2 cut(s) 120, 954
PshAI GACNNNNGTC 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 561, 882
PspEI GGTNACC 1 cut(s) 653
PspFI CCCAGC 2 cut(s) 385, 781
PspGI CCWGG 2 cut(s) 561, 882
PspN4I GGNNCC 5 cut(s) 425, 469, 527, 727, 894
PspOMI GGGCCC 1 cut(s) 525
PspPI GGNCC 3 cut(s) 455, 525, 526
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 572
PvuII CAGCTG 1 cut(s) 382
RsaI GTAC 4 cut(s) 329, 425, 686, 830
RsaNI GTAC 4 cut(s) 328, 424, 685, 829
RseI CAYNNNNRTG 1 cut(s) 534
SapI GCTCTTC 1 cut(s) 563
SaqAI TTAA 2 cut(s) 927, 954
SatI GCNGC 3 cut(s) 380, 383, 544
Sau3AI GATC 4 cut(s) 313, 510, 669, 774
Sau96I GGNCC 3 cut(s) 455, 525, 526
SchI GAGTC 2 cut(s) 121, 955
ScrFI CCNGG 3 cut(s) 524, 563, 884
SduI GDGCHC 3 cut(s) 168, 529, 702
SfaNI GCATC 4 cut(s) 67, 214, 526, 757
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 3 cut(s) 317, 593, 662
SmoI CTYRAG 3 cut(s) 317, 593, 662
Sse9I AATT 2 cut(s) 368, 836
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 1 cut(s) 543
SspMI CTAG 1 cut(s) 794
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 522, 561, 882
StyI CCWWGG 3 cut(s) 471, 649, 888
TaaI ACNGT 1 cut(s) 145
TasI AATT 2 cut(s) 368, 836
TatI WGTACW 2 cut(s) 327, 684
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 3 cut(s) 121, 403, 820
Tru1I TTAA 2 cut(s) 927, 954
Tru9I TTAA 2 cut(s) 927, 954
TscAI CASTG 7 cut(s) 150, 182, 583, 769, 777, 816, 924
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 379, 382
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 4 cut(s) 356, 735, 872, 926
TspGWI ACGGA 1 cut(s) 615
TspRI CASTG 7 cut(s) 150, 182, 583, 769, 777, 816, 924
Tth111I GACNNNGTC 1 cut(s) 572
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 455
XagI CCTNNNNNAGG 1 cut(s) 431
XapI RAATTY 1 cut(s) 836
XspI CTAG 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.