Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0013787

Physical Location & Seq

Genomic Coordinates Reverse (-)

13857818 .. 13858879

Gene Structure 1062 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1150400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGCACCACCTCTCCGAGCACACGCAAGTAGTCAGAATCCGCGGCACCTACGAGGACGCCGCCGCCGTCCATTTGGTCATGGAGCTCTGCCGCGGCGGTGAGCTTTTCGATAGGATCATGAAGAAAGGGCATTACAGCGAGCGGGAGGCGGCGAAGCTTCTGAAAACCATTGTTGGGGTTGTGGAGGCTTGTCATTCGCTTAGGGTTATGCATTGGGATTTGAAGCCGGAGAACTTCTTGTTCAAAAGCGAGAAGGAGGACGCGGCGCTGAAGGCGGTTTTCTACAAGCCCGGTGAGACCTTTTCTGAAGTTGTTGGAAGTAAATATTACGTTGCCCCTGAGGTGCTGAGGAAGCACTACGGGCCGGAATCGGATGTGTGGAGCGCAGGGGTGATTCTGTACATTTTGCTAAGTGAGGTTCTGCCGTTCTGGGCGGAGACGGAGATTGGGATCTTCCGGCAGATTTTGCAGGGGAGGTTGGATTTTGAGTCCGAGCCGTGGCCTGGGATTTCTAGTAGTGCACGATTGCAGAAGAGAATCACTGCCCATGAGGTGTTTTGCCATCCGTGGATCATAGATGACACGATTGCGCTCGATAAGCCGCTGGACTCGGCTGTCCTGTCGAGGATGAAGCAGTTCTCCACCATGAACAAGCTCAAGAAAATGGCGCTGAGAGTGATCGCTGAGCGGCTTTCGGAGGAGGAGATTGGCGGGCTGAGGGAGATTTTTAAGATGATTGATGCAGATGGGAGCGGGAGCATCACGTTTGATGAGCTCAAAGAGGGGCTGAGGAAAGTAGGGTCTGAGCTAATGGAGTTTGAGATCAAGGATCTAATGAAGGCAGCTGACATTGACAGCAGCGAGACAATCGACTACGGCGAGTTTCTGGCGGCGACAGTGCACTTGAACAAGCTGGAGAGGGAGGAGAATCTACTGTCTGCTTTCTCATTTTTCGACAAGGACGGAAGCGGGTTCATCACCATTGATGAGCTGAGTCAAGCTTGCAGGGAGTTTGGATTGGGGGAGCTGCATCTGGAGGACATGATCAAGGAGATTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

354

Amino Acids

40.14

Weight (kDa)

5.15

Isoelectric Point (pI)

44.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	1 - 138	7.4e-10	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	2 - 191	1.7e-40	Protein kinase domain
EF-hand_1	PF00036	239 - 265	4.9e-08	EF hand domain
EF-hand_6	PF13405	239 - 267	9.3e-08	EF-hand domain
EF-hand_7	PF13499	239 - 298	9.6e-11	EF-hand domain pair
EF-hand_5	PF13202	239 - 261	8e-06	EF hand
EF-hand_7	PF13499	308 - 353	3.2e-06	EF-hand domain pair
EF-hand_6	PF13405	311 - 339	4.6e-07	EF-hand domain
EF-hand_1	PF00036	313 - 337	3.5e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013787)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23580
fragaria_vesca	FvH4_6g35360
malus_domestica	MD09G1176400.v1.1 MD17G1150400.v1.1
prunus_persica	Prupe.3G035400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0146801
rosa_laevigata	RLG00000020223
rosa_multiflora	Rmu_co8336567.1_g000001 Rmu_sc0007829.1_g000006 Rmu_sc0015268.1_g000005 Rmu_sc0017620.1_g000002
rosa_roxburghii	Rroxscaffold_2G00099900
rosa_rugosa	Rorug02G0397200
rosa_samantha	Rh2AG453400 Rh2CG440500 Rh2DG475300
rosa_wichuraiana	Rw2G037010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	614
AccB1I	GGYRCC	1 cut(s)	44
AccBSI	CCGCTC	3 cut(s)	142, 688, 753
AccII	CGCG	3 cut(s)	42, 93, 263
AclWI	GGATC	4 cut(s)	122, 458, 578, 837
AcuI	CTGAAG	2 cut(s)	290, 327
AcyI	GRCGYC	1 cut(s)	57
AfaI	GTAC	1 cut(s)	401
AfiI	CCNNNNNNNGG	3 cut(s)	174, 498, 503
AgsI	TTSAA	3 cut(s)	223, 244, 907
AjnI	CCWGG	1 cut(s)	502
Alw21I	GWGCWC	5 cut(s)	21, 87, 523, 777, 903
Alw26I	GTCTC	3 cut(s)	290, 431, 857
Alw44I	GTGCAC	2 cut(s)	519, 899
AlwI	GGATC	4 cut(s)	122, 458, 578, 837
AoxI	GGCC	2 cut(s)	362, 500
ApaLI	GTGCAC	2 cut(s)	519, 899
ApeKI	GCWGC	3 cut(s)	842, 858, 1027
Asp700I	GAANNNNTTC	1 cut(s)	635
AspLEI	GCGC	4 cut(s)	268, 386, 592, 670
AspS9I	GGNCC	1 cut(s)	362
AsuC2I	CCSGG	1 cut(s)	291
AsuHPI	GGTGA	4 cut(s)	110, 305, 403, 970
AxyI	CCTNAGG	1 cut(s)	339
BaeGI	GKGCMC	2 cut(s)	523, 903
BanI	GGYRCC	1 cut(s)	44
BanII	GRGCYC	2 cut(s)	87, 777
Bbv12I	GWGCWC	5 cut(s)	21, 87, 523, 777, 903
BbvCI	CCTCAGC	3 cut(s)	347, 716, 788
BbvI	GCAGC	3 cut(s)	854, 870, 1014
BccI	CCATC	2 cut(s)	570, 740
BceAI	ACGGC	4 cut(s)	50, 409, 481, 892
BciT130I	CCWGG	1 cut(s)	504
BclI	TGATCA	1 cut(s)	1044
BcnI	CCSGG	1 cut(s)	291
BcoDI	GTCTC	3 cut(s)	290, 431, 857
BfaI	CTAG	1 cut(s)	513
BfoI	RGCGCY	2 cut(s)	269, 671
BlpI	GCTNAGC	1 cut(s)	684
Bme1390I	CCNGG	2 cut(s)	291, 504
BmgT120I	GGNCC	1 cut(s)	362
BmiI	GGNNCC	1 cut(s)	46
BmrFI	CCNGG	2 cut(s)	291, 504
BmsI	GCATC	3 cut(s)	730, 768, 1039
BpmI	CTGGAG	2 cut(s)	935, 1055
Bpu10I	CCTNAGC	4 cut(s)	200, 347, 716, 788
Bpu1102I	GCTNAGC	1 cut(s)	684
BpuEI	CTTGAG	1 cut(s)	641
BpuMI	CCSGG	1 cut(s)	291
BsaBI	GATNNNNATC	1 cut(s)	449
BsaHI	GRCGYC	1 cut(s)	57
BsaI	GGTCTC	1 cut(s)	290
BsaJI	CCNNGG	5 cut(s)	40, 91, 497, 503, 566
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	3 cut(s)	174, 498, 503
Bse21I	CCTNAGG	1 cut(s)	339
Bse8I	GATNNNNATC	1 cut(s)	449
BseBI	CCWGG	1 cut(s)	504
BseDI	CCNNGG	5 cut(s)	40, 91, 497, 503, 566
BseGI	GGATG	3 cut(s)	379, 562, 633
BseJI	GATNNNNATC	1 cut(s)	449
BseLI	CCNNNNNNNGG	3 cut(s)	174, 498, 503
BseMII	CTCAG	8 cut(s)	330, 338, 662, 675, 707, 779, 795, 983
BseRI	GAGGAG	3 cut(s)	713, 716, 938
BseSI	GKGCMC	2 cut(s)	523, 903
BseXI	GCAGC	3 cut(s)	854, 870, 1014
Bsh1236I	CGCG	3 cut(s)	42, 93, 263
BshFI	GGCC	2 cut(s)	364, 502
BshNI	GGYRCC	1 cut(s)	44
BsiHKAI	GWGCWC	5 cut(s)	21, 87, 523, 777, 903
BsiSI	CCGG	4 cut(s)	227, 291, 365, 457
BslI	CCNNNNNNNGG	3 cut(s)	174, 498, 503
BsmAI	GTCTC	3 cut(s)	290, 431, 857
BsmBI	CGTCTC	1 cut(s)	431
BsnI	GGCC	2 cut(s)	364, 502
Bso31I	GGTCTC	1 cut(s)	290
Bsp1286I	GDGCHC	5 cut(s)	21, 87, 523, 777, 903
Bsp1407I	TGTACA	1 cut(s)	399
Bsp143I	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
Bsp1720I	GCTNAGC	1 cut(s)	684
BspANI	GGCC	2 cut(s)	364, 502
BspCNI	CTCAG	8 cut(s)	331, 339, 663, 676, 708, 780, 796, 984
BspFNI	CGCG	3 cut(s)	42, 93, 263
BspHI	TCATGA	1 cut(s)	117
BspLI	GGNNCC	1 cut(s)	46
BspPI	GGATC	4 cut(s)	122, 458, 578, 837
BspT107I	GGYRCC	1 cut(s)	44
BspTNI	GGTCTC	1 cut(s)	290
BsrBI	CCGCTC	3 cut(s)	142, 688, 753
BsrGI	TGTACA	1 cut(s)	399
BssECI	CCNNGG	5 cut(s)	40, 91, 497, 503, 566
BssMI	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
BssNI	GRCGYC	1 cut(s)	57
Bst2UI	CCWGG	1 cut(s)	504
Bst4CI	ACNGT	2 cut(s)	898, 936
Bst6I	CTCTTC	1 cut(s)	527
BstACI	GRCGYC	1 cut(s)	57
BstAPI	GCANNNNNTGC	1 cut(s)	466
BstAUI	TGTACA	1 cut(s)	399
BstC8I	GCNNGC	3 cut(s)	140, 713, 1003
BstDSI	CCRYGG	4 cut(s)	40, 91, 497, 566
BstF5I	GGATG	3 cut(s)	379, 562, 633
BstFNI	CGCG	3 cut(s)	42, 93, 263
BstH2I	RGCGCY	2 cut(s)	269, 671
BstHHI	GCGC	4 cut(s)	268, 386, 592, 670
BstKTI	GATC	7 cut(s)	117, 453, 573, 681, 825, 832, 1047
BstMAI	GTCTC	3 cut(s)	290, 431, 857
BstMBI	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
BstMWI	GCNNNNNNNGC	5 cut(s)	272, 352, 361, 466, 598
BstNI	CCWGG	1 cut(s)	504
BstSCI	CCNGG	2 cut(s)	289, 502
BstSLI	GKGCMC	2 cut(s)	523, 903
BstUI	CGCG	3 cut(s)	42, 93, 263
BstV1I	GCAGC	3 cut(s)	854, 870, 1014
BstX2I	RGATCY	2 cut(s)	450, 829
BstYI	RGATCY	2 cut(s)	450, 829
Bsu36I	CCTNAGG	1 cut(s)	339
BsuRI	GGCC	2 cut(s)	364, 502
BtgI	CCRYGG	4 cut(s)	40, 91, 497, 566
BtsCI	GGATG	3 cut(s)	379, 562, 633
BtsI	GCAGTG	1 cut(s)	540
BtsIMutI	CAGTG	2 cut(s)	540, 903
Cac8I	GCNNGC	3 cut(s)	140, 713, 1003
CciI	TCATGA	1 cut(s)	117
CfoI	GCGC	4 cut(s)	268, 386, 592, 670
Cfr13I	GGNCC	1 cut(s)	362
Cfr42I	CCGCGG	2 cut(s)	43, 94
CseI	GACGC	2 cut(s)	65, 269
Csp6I	GTAC	1 cut(s)	400
CviAII	CATG	5 cut(s)	79, 118, 548, 646, 1042
CviQI	GTAC	1 cut(s)	400
DpnI	GATC	7 cut(s)	116, 452, 572, 680, 824, 831, 1046
DpnII	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
DrdI	GACNNNNNNGTC	1 cut(s)	614
DseDI	GACNNNNNNGTC	1 cut(s)	614
Eam1104I	CTCTTC	1 cut(s)	527
EarI	CTCTTC	1 cut(s)	527
EciI	GGCGGA	1 cut(s)	449
Ecl136II	GAGCTC	2 cut(s)	85, 775
Eco24I	GRGCYC	2 cut(s)	87, 777
Eco31I	GGTCTC	1 cut(s)	290
Eco53kI	GAGCTC	2 cut(s)	85, 775
Eco57I	CTGAAG	2 cut(s)	290, 327
Eco81I	CCTNAGG	1 cut(s)	339
EcoICRI	GAGCTC	2 cut(s)	85, 775
EcoRII	CCWGG	1 cut(s)	502
EcoT22I	ATGCAT	1 cut(s)	213
EcoT38I	GRGCYC	2 cut(s)	87, 777
Esp3I	CGTCTC	1 cut(s)	431
FaeI	CATG	5 cut(s)	82, 121, 551, 649, 1045
FaiI	YATR	7 cut(s)	80, 119, 209, 549, 575, 647, 1043
FatI	CATG	5 cut(s)	78, 117, 547, 645, 1041
FauI	CCCGC	4 cut(s)	135, 704, 746, 962
FbaI	TGATCA	1 cut(s)	1044
FokI	GGATG	3 cut(s)	386, 549, 640
FriOI	GRGCYC	2 cut(s)	87, 777
FspBI	CTAG	1 cut(s)	513
GlaI	GCGC	4 cut(s)	267, 385, 591, 669
GsuI	CTGGAG	2 cut(s)	935, 1055
HaeII	RGCGCY	2 cut(s)	269, 671
HaeIII	GGCC	2 cut(s)	364, 502
HapII	CCGG	4 cut(s)	227, 291, 365, 457
HgaI	GACGC	2 cut(s)	65, 269
HhaI	GCGC	4 cut(s)	268, 386, 592, 670
Hin1I	GRCGYC	1 cut(s)	57
Hin1II	CATG	5 cut(s)	82, 121, 551, 649, 1045
Hin6I	GCGC	4 cut(s)	266, 384, 590, 668
HinP1I	GCGC	4 cut(s)	266, 384, 590, 668
HindIII	AAGCTT	2 cut(s)	155, 999
HinfI	GANTC	8 cut(s)	36, 368, 394, 488, 537, 608, 928, 994
HpaII	CCGG	4 cut(s)	227, 291, 365, 457
HphI	GGTGA	4 cut(s)	110, 305, 403, 970
Hpy166II	GTNNAC	2 cut(s)	521, 901
Hpy188I	TCNGA	8 cut(s)	16, 35, 162, 307, 373, 493, 697, 805
Hpy188III	TCNNGA	3 cut(s)	118, 658, 1034
Hpy8I	GTNNAC	2 cut(s)	521, 901
HpyAV	CCTTC	3 cut(s)	247, 265, 832
HpyCH4III	ACNGT	2 cut(s)	898, 936
HpyCH4IV	ACGT	2 cut(s)	330, 764
HpyCH4V	TGCA	9 cut(s)	4, 211, 469, 521, 529, 743, 901, 1005, 1030
HpyF10VI	GCNNNNNNNGC	5 cut(s)	272, 352, 361, 466, 598
HpySE526I	ACGT	2 cut(s)	330, 764
Hsp92I	GRCGYC	1 cut(s)	57
Hsp92II	CATG	5 cut(s)	82, 121, 551, 649, 1045
HspAI	GCGC	4 cut(s)	266, 384, 590, 668
Ksp22I	TGATCA	1 cut(s)	1044
KspI	CCGCGG	2 cut(s)	43, 94
Kzo9I	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
LmnI	GCTCC	5 cut(s)	82, 381, 750, 756, 1024
Lsp1109I	GCAGC	3 cut(s)	854, 870, 1014
LweI	GCATC	3 cut(s)	730, 768, 1039
MaeI	CTAG	1 cut(s)	513
MaeII	ACGT	2 cut(s)	330, 764
MalI	GATC	7 cut(s)	116, 452, 572, 680, 824, 831, 1046
MbiI	CCGCTC	3 cut(s)	142, 688, 753
MboI	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
MboII	GAAGA	3 cut(s)	133, 445, 544
MflI	RGATCY	2 cut(s)	450, 829
MhlI	GDGCHC	5 cut(s)	21, 87, 523, 777, 903
MlyI	GAGTC	3 cut(s)	497, 602, 1003
MmeI	TCCRAC	2 cut(s)	295, 459
Mph1103I	ATGCAT	1 cut(s)	213
MroXI	GAANNNNTTC	1 cut(s)	635
MseI	TTAA	1 cut(s)	729
MspA1I	CMGCKG	4 cut(s)	42, 93, 604, 845
MspI	CCGG	4 cut(s)	227, 291, 365, 457
MspR9I	CCNGG	2 cut(s)	291, 504
MvaI	CCWGG	1 cut(s)	504
MvnI	CGCG	3 cut(s)	42, 93, 263
MwoI	GCNNNNNNNGC	5 cut(s)	272, 352, 361, 466, 598
NciI	CCSGG	1 cut(s)	291
NdeII	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
NlaIII	CATG	5 cut(s)	82, 121, 551, 649, 1045
NlaIV	GGNNCC	1 cut(s)	46
NmeAIII	GCCGAG	1 cut(s)	590
NsiI	ATGCAT	1 cut(s)	213
PagI	TCATGA	1 cut(s)	117
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 876
PdmI	GAANNNNTTC	1 cut(s)	635
PfeI	GAWTC	5 cut(s)	36, 368, 394, 537, 928
PleI	GAGTC	3 cut(s)	496, 602, 1002
PpsI	GAGTC	3 cut(s)	496, 602, 1002
Psp124BI	GAGCTC	2 cut(s)	87, 777
Psp6I	CCWGG	1 cut(s)	502
PspGI	CCWGG	1 cut(s)	502
PspN4I	GGNNCC	1 cut(s)	46
PspPI	GGNCC	1 cut(s)	362
PsuI	RGATCY	2 cut(s)	450, 829
PvuII	CAGCTG	1 cut(s)	845
RsaI	GTAC	1 cut(s)	401
RsaNI	GTAC	1 cut(s)	400
SacI	GAGCTC	2 cut(s)	87, 777
SacII	CCGCGG	2 cut(s)	43, 94
SaqAI	TTAA	1 cut(s)	729
Sau3AI	GATC	7 cut(s)	114, 450, 570, 678, 822, 829, 1044
Sau96I	GGNCC	1 cut(s)	362
SchI	GAGTC	3 cut(s)	497, 602, 1003
ScrFI	CCNGG	2 cut(s)	291, 504
SduI	GDGCHC	5 cut(s)	21, 87, 523, 777, 903
SfaNI	GCATC	3 cut(s)	730, 768, 1039
Sfr303I	CCGCGG	2 cut(s)	43, 94
SgrBI	CCGCGG	2 cut(s)	43, 94
SmlI	CTYRAG	1 cut(s)	656
SmoI	CTYRAG	1 cut(s)	656
SspI	AATATT	1 cut(s)	326
SspMI	CTAG	1 cut(s)	513
SstI	GAGCTC	2 cut(s)	87, 777
StyD4I	CCNGG	2 cut(s)	289, 502
TaaI	ACNGT	2 cut(s)	898, 936
TaiI	ACGT	2 cut(s)	333, 767
TaqI	TCGA	5 cut(s)	108, 594, 623, 870, 954
TatI	WGTACW	1 cut(s)	399
TfiI	GAWTC	5 cut(s)	36, 368, 394, 537, 928
Tru1I	TTAA	1 cut(s)	729
Tru9I	TTAA	1 cut(s)	729
TscAI	CASTG	2 cut(s)	547, 903
TseI	GCWGC	3 cut(s)	842, 858, 1027
TspDTI	ATGAA	5 cut(s)	134, 644, 662, 851, 964
TspGWI	ACGGA	3 cut(s)	455, 555, 978
TspRI	CASTG	2 cut(s)	547, 903
VneI	GTGCAC	2 cut(s)	519, 899
XmnI	GAANNNNTTC	1 cut(s)	635
XspI	CTAG	1 cut(s)	513
Zsp2I	ATGCAT	1 cut(s)	213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.