Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0014409

Physical Location & Seq

Genomic Coordinates Reverse (-)

20109164 .. 20113348

Gene Structure 4185 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1177100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 771 bp

ATGACGGACTATATACAAGAGCAGGAGATGGAAATTGAAGCGTTGGAAGCAATACTTGCGGATGACTTCAAAGAAATACATTCGGGTGAGAGCGGGTTAAATACTTCCAAGAGGTGTTTTCAAATAAAAGTATCTCCAGTGGATGATGAAGCAGATGAATTAACAACCAATCCAGTTCAGCTGGCTTTGATTTTCTCACACACAGAAAGATACCCAGATGAACCTCCACTTTTGAATTTGAATAGTTTACGCGGTATACAAGGAGAACATCTAAGAATTTTGAAAGAAAAGCTTCTGCAAGAGGCATCTGAAAACCTTGGTATGGCTATGATCTACACACTAGTTACATCAGCCAAGGAATGGCTTTCTGAACAATATTGTCAAGACACTAGTCTTGAGGATGCTGAAGCAGAAGAAGCAGCAAAGGATGATGTAATCATACCACATGGAGAACCAGTTACAGTTGAAACATTTCTGGCATGGAGAGAGATGTTTGAAGCAGAGTTGGCCCTGGAACGAGCCAAGTTAATGCCTGACGCTGCACTTTCAGCACCCAAGGAAAAGAGGCTCACAGGTAGACAGTGGTTTGAAACCGGAAGACATGCTGTGAAAGGTGCAGCTCCAGCCTCTGAAGGGTCCGATGAAGAGGATGAGGACGAGATTGACTTCGATTCTGATGATTTTGAAGATGACGAAGATGATATGCTGGAACACTATTTGGCTGAGAAGTCGGACTCATCAACTCACTCTTCGAAGAGGGTCAATAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005844 GO:0006355 GO:0006357 GO:0006412 GO:0006518 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007568 GO:0007569 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010469 GO:0010556 GO:0010646 GO:0010647 GO:0014070 GO:0019219 GO:0019222 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031326 GO:0032502 GO:0032870 GO:0032991 GO:0033143 GO:0033145 GO:0033554 GO:0033574 GO:0033993 GO:0034599 GO:0034641 GO:0034645 GO:0042221 GO:0043043 GO:0043170 GO:0043401 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0048518 GO:0048522 GO:0048545 GO:0048583 GO:0048584 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0060765 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071383 GO:0071394 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0080090 GO:1901564 GO:1901566 GO:1901576 GO:1901654 GO:1901655 GO:1901700 GO:1901701 GO:1903506 GO:1990904 GO:2000112 GO:2000273 GO:2000823 GO:2000825 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RWD

Protein Analysis

257

Amino Acids

28.91

Weight (kDa)

4.27

Isoelectric Point (pI)

42.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	6 - 122	8.4e-25	RWD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014409)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51730
fragaria_vesca	FvH4_6g32940
malus_domestica	MD09G1196300.v1.1 MD17G1177100.v1.1
prunus_persica	Prupe.3G052700_v2.0.a1
pyrus_communis	pycom09g11340 pycom17g18600
rosa_chinensis	RchiOBHm_Chr2g0142451
rosa_multiflora	Rmu_sc0001211.1_g000015 Rmu_sc0005700.1_g000001
rosa_roxburghii	Rroxscaffold_2G00102590
rosa_rugosa	Rorug02G0372900
rosa_samantha	Rh2AG424500 Rh2BG434200 Rh2CG411100 Rh2DG444200
rosa_wichuraiana	Rw2G034770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	360
AccBSI	CCGCTC	1 cut(s)	93
AccI	GTMKAC	2 cut(s)	256, 577
AccII	CGCG	1 cut(s)	252
AciI	CCGC	3 cut(s)	59, 93, 252
AcsI	RAATTY	2 cut(s)	235, 276
AcuI	CTGAAG	2 cut(s)	426, 651
AfiI	CCNNNNNNNGG	3 cut(s)	322, 360, 633
AhlI	ACTAGT	2 cut(s)	340, 389
AjnI	CCWGG	1 cut(s)	510
AluBI	AGCT	3 cut(s)	181, 292, 620
AluI	AGCT	3 cut(s)	181, 292, 620
AlwNI	CAGNNNCTG	1 cut(s)	629
AoxI	GGCC	1 cut(s)	507
ApeKI	GCWGC	3 cut(s)	419, 539, 617
ApoI	RAATTY	2 cut(s)	235, 276
Asp700I	GAANNNNTTC	2 cut(s)	291, 471
AspS9I	GGNCC	2 cut(s)	508, 636
AsuHPI	GGTGA	1 cut(s)	98
AsuII	TTCGAA	1 cut(s)	752
AvaII	GGWCC	1 cut(s)	636
BbsI	GAAGAC	1 cut(s)	604
BbvI	GCAGC	3 cut(s)	431, 526, 629
BccI	CCATC	1 cut(s)	22
BciT130I	CCWGG	1 cut(s)	512
BcuI	ACTAGT	2 cut(s)	340, 389
BfaI	CTAG	3 cut(s)	341, 390, 769
BisI	GCNGC	3 cut(s)	420, 540, 618
BlsI	GCNGC	3 cut(s)	421, 541, 619
Bme1390I	CCNGG	1 cut(s)	512
Bme18I	GGWCC	1 cut(s)	636
BmgT120I	GGNCC	2 cut(s)	508, 636
BmiI	GGNNCC	1 cut(s)	637
BmrFI	CCNGG	1 cut(s)	512
BmsI	GCATC	2 cut(s)	314, 391
BoxI	GACNNNNGTC	1 cut(s)	390
BpiI	GAAGAC	1 cut(s)	604
BpmI	CTGGAG	2 cut(s)	120, 606
Bpu14I	TTCGAA	1 cut(s)	752
BpuEI	CTTGAG	1 cut(s)	416
BsaJI	CCNNGG	4 cut(s)	316, 354, 510, 555
BsaWI	WCCGGW	1 cut(s)	593
BsaXI	ACNNNNNCTCC	2 cut(s)	441, 471
Bsc4I	CCNNNNNNNGG	3 cut(s)	322, 360, 633
Bse1I	ACTGG	3 cut(s)	137, 173, 455
BseBI	CCWGG	1 cut(s)	512
BseDI	CCNNGG	4 cut(s)	316, 354, 510, 555
BseGI	GGATG	5 cut(s)	67, 148, 406, 433, 655
BseLI	CCNNNNNNNGG	3 cut(s)	322, 360, 633
BseMII	CTCAG	1 cut(s)	714
BseNI	ACTGG	3 cut(s)	137, 173, 455
BseXI	GCAGC	3 cut(s)	431, 526, 629
BsgI	GTGCAG	2 cut(s)	525, 636
Bsh1236I	CGCG	1 cut(s)	252
BshFI	GGCC	1 cut(s)	509
BsiSI	CCGG	1 cut(s)	594
BslI	CCNNNNNNNGG	3 cut(s)	322, 360, 633
BsnI	GGCC	1 cut(s)	509
Bsp119I	TTCGAA	1 cut(s)	752
Bsp143I	GATC	1 cut(s)	330
BspACI	CCGC	3 cut(s)	59, 93, 252
BspANI	GGCC	1 cut(s)	509
BspCNI	CTCAG	1 cut(s)	715
BspFNI	CGCG	1 cut(s)	252
BspLI	GGNNCC	1 cut(s)	637
BspT104I	TTCGAA	1 cut(s)	752
BsrBI	CCGCTC	1 cut(s)	93
BsrI	ACTGG	3 cut(s)	137, 173, 455
BssECI	CCNNGG	4 cut(s)	316, 354, 510, 555
BssMI	GATC	1 cut(s)	330
BssNAI	GTATAC	1 cut(s)	257
BssT1I	CCWWGG	3 cut(s)	316, 354, 555
Bst1107I	GTATAC	1 cut(s)	257
Bst2UI	CCWGG	1 cut(s)	512
Bst4CI	ACNGT	2 cut(s)	463, 582
Bst6I	CTCTTC	3 cut(s)	639, 749, 754
BstAPI	GCANNNNNTGC	1 cut(s)	56
BstBI	TTCGAA	1 cut(s)	752
BstC8I	GCNNGC	1 cut(s)	183
BstDEI	CTNAG	2 cut(s)	272, 723
BstF5I	GGATG	5 cut(s)	67, 148, 406, 433, 655
BstFNI	CGCG	1 cut(s)	252
BstKTI	GATC	1 cut(s)	333
BstMBI	GATC	1 cut(s)	330
BstMWI	GCNNNNNNNGC	6 cut(s)	47, 56, 416, 506, 548, 623
BstNI	CCWGG	1 cut(s)	512
BstNSI	RCATGY	1 cut(s)	605
BstPAI	GACNNNNGTC	1 cut(s)	390
BstSCI	CCNGG	1 cut(s)	510
BstUI	CGCG	1 cut(s)	252
BstV1I	GCAGC	3 cut(s)	431, 526, 629
BstV2I	GAAGAC	1 cut(s)	604
BstZ17I	GTATAC	1 cut(s)	257
BsuRI	GGCC	1 cut(s)	509
BtsCI	GGATG	5 cut(s)	67, 148, 406, 433, 655
BtsIMutI	CAGTG	2 cut(s)	144, 587
Cac8I	GCNNGC	1 cut(s)	183
CaiI	CAGNNNCTG	1 cut(s)	629
Cfr13I	GGNCC	2 cut(s)	508, 636
CseI	GACGC	1 cut(s)	545
CviAII	CATG	3 cut(s)	446, 480, 602
DdeI	CTNAG	2 cut(s)	272, 723
DpnI	GATC	1 cut(s)	332
DpnII	GATC	1 cut(s)	330
Eam1104I	CTCTTC	3 cut(s)	639, 749, 754
EarI	CTCTTC	3 cut(s)	639, 749, 754
Eco130I	CCWWGG	3 cut(s)	316, 354, 555
Eco47I	GGWCC	1 cut(s)	636
Eco57I	CTGAAG	2 cut(s)	426, 651
EcoRII	CCWGG	1 cut(s)	510
EcoT14I	CCWWGG	3 cut(s)	316, 354, 555
ErhI	CCWWGG	3 cut(s)	316, 354, 555
FaeI	CATG	3 cut(s)	449, 483, 605
FalI	AAGNNNNNCTT	4 cut(s)	39, 71, 276, 308
FatI	CATG	3 cut(s)	445, 479, 601
FauI	CCCGC	1 cut(s)	86
FblI	GTMKAC	2 cut(s)	256, 577
Fnu4HI	GCNGC	3 cut(s)	420, 540, 618
FokI	GGATG	5 cut(s)	74, 155, 413, 440, 662
Fsp4HI	GCNGC	3 cut(s)	420, 540, 618
FspBI	CTAG	3 cut(s)	341, 390, 769
GluI	GCNGC	3 cut(s)	420, 540, 618
GsuI	CTGGAG	2 cut(s)	120, 606
HaeIII	GGCC	1 cut(s)	509
HapII	CCGG	1 cut(s)	594
HgaI	GACGC	1 cut(s)	545
Hin1II	CATG	3 cut(s)	449, 483, 605
HindIII	AAGCTT	1 cut(s)	290
HinfI	GANTC	2 cut(s)	671, 734
HpaII	CCGG	1 cut(s)	594
HphI	GGTGA	1 cut(s)	98
Hpy166II	GTNNAC	3 cut(s)	248, 257, 578
Hpy188I	TCNGA	6 cut(s)	310, 370, 631, 640, 676, 733
Hpy188III	TCNNGA	2 cut(s)	383, 395
Hpy8I	GTNNAC	3 cut(s)	248, 257, 578
HpyAV	CCTTC	1 cut(s)	626
HpyCH4III	ACNGT	2 cut(s)	463, 582
HpyCH4V	TGCA	3 cut(s)	298, 542, 617
HpyF10VI	GCNNNNNNNGC	6 cut(s)	47, 56, 416, 506, 548, 623
HpyF3I	CTNAG	2 cut(s)	272, 723
Hsp92II	CATG	3 cut(s)	449, 483, 605
Kzo9I	GATC	1 cut(s)	330
LmnI	GCTCC	1 cut(s)	625
Lsp1109I	GCAGC	3 cut(s)	431, 526, 629
LweI	GCATC	2 cut(s)	314, 391
MaeI	CTAG	3 cut(s)	341, 390, 769
MaeIII	GTNAC	2 cut(s)	343, 457
MalI	GATC	1 cut(s)	332
MbiI	CCGCTC	1 cut(s)	93
MboI	GATC	1 cut(s)	330
MboII	GAAGA	7 cut(s)	425, 609, 656, 698, 707, 741, 766
MluCI	AATT	4 cut(s)	33, 158, 235, 276
MlyI	GAGTC	1 cut(s)	728
MmeI	TCCRAC	2 cut(s)	24, 711
MnlI	CCTC	9 cut(s)	105, 234, 295, 391, 558, 637, 640, 646, 750
MroXI	GAANNNNTTC	2 cut(s)	291, 471
MseI	TTAA	3 cut(s)	98, 161, 527
MslI	CAYNNNNRTG	1 cut(s)	84
MspA1I	CMGCKG	1 cut(s)	181
MspI	CCGG	1 cut(s)	594
MspR9I	CCNGG	1 cut(s)	512
MvaI	CCWGG	1 cut(s)	512
MvnI	CGCG	1 cut(s)	252
MwoI	GCNNNNNNNGC	6 cut(s)	47, 56, 416, 506, 548, 623
NdeII	GATC	1 cut(s)	330
NlaIII	CATG	3 cut(s)	449, 483, 605
NlaIV	GGNNCC	1 cut(s)	637
NspI	RCATGY	1 cut(s)	605
NspV	TTCGAA	1 cut(s)	752
PdmI	GAANNNNTTC	2 cut(s)	291, 471
PfeI	GAWTC	1 cut(s)	671
PflMI	CCANNNNNTGG	1 cut(s)	360
PkrI	GCNGC	3 cut(s)	421, 541, 619
PleI	GAGTC	1 cut(s)	728
PpsI	GAGTC	1 cut(s)	728
PshAI	GACNNNNGTC	1 cut(s)	390
Psp6I	CCWGG	1 cut(s)	510
PspGI	CCWGG	1 cut(s)	510
PspN4I	GGNNCC	1 cut(s)	637
PspPI	GGNCC	2 cut(s)	508, 636
PstNI	CAGNNNCTG	1 cut(s)	629
PvuII	CAGCTG	1 cut(s)	181
RseI	CAYNNNNRTG	1 cut(s)	84
SaqAI	TTAA	3 cut(s)	98, 161, 527
SatI	GCNGC	3 cut(s)	420, 540, 618
Sau3AI	GATC	1 cut(s)	330
Sau96I	GGNCC	2 cut(s)	508, 636
SchI	GAGTC	1 cut(s)	728
ScrFI	CCNGG	1 cut(s)	512
SetI	ASST	8 cut(s)	116, 183, 226, 294, 318, 577, 616, 622
SfaNI	GCATC	2 cut(s)	314, 391
SfuI	TTCGAA	1 cut(s)	752
SinI	GGWCC	1 cut(s)	636
SmiMI	CAYNNNNRTG	1 cut(s)	84
SmlI	CTYRAG	1 cut(s)	395
SmoI	CTYRAG	1 cut(s)	395
SpeI	ACTAGT	2 cut(s)	340, 389
Sse9I	AATT	4 cut(s)	33, 158, 235, 276
SsiI	CCGC	3 cut(s)	59, 93, 252
SspI	AATATT	1 cut(s)	377
SspMI	CTAG	3 cut(s)	341, 390, 769
StyD4I	CCNGG	1 cut(s)	510
StyI	CCWWGG	3 cut(s)	316, 354, 555
TaaI	ACNGT	2 cut(s)	463, 582
TaqI	TCGA	2 cut(s)	669, 752
TasI	AATT	4 cut(s)	33, 158, 235, 276
TfiI	GAWTC	1 cut(s)	671
Tru1I	TTAA	3 cut(s)	98, 161, 527
Tru9I	TTAA	3 cut(s)	98, 161, 527
TscAI	CASTG	2 cut(s)	144, 587
TseI	GCWGC	3 cut(s)	419, 539, 617
TspDTI	ATGAA	4 cut(s)	162, 171, 234, 657
TspGWI	ACGGA	1 cut(s)	20
TspRI	CASTG	2 cut(s)	144, 587
Van91I	CCANNNNNTGG	1 cut(s)	360
VpaK11BI	GGWCC	1 cut(s)	636
XapI	RAATTY	2 cut(s)	235, 276
XceI	RCATGY	1 cut(s)	605
XmiI	GTMKAC	2 cut(s)	256, 577
XmnI	GAANNNNTTC	2 cut(s)	291, 471
XspI	CTAG	3 cut(s)	341, 390, 769

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.