MD17G1194900.v1.1

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017171
Physical Location & Seq
Forward (+)
23359467 .. 23360330
864 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1194900.v1.1.491

Sequence Viewer

Length: 864 bp
ATGCCCAATAAACTCCAAAAGTATCTCCAAGACTACCTCTCCAAGATAAAAAAGCCACCCCCACAGATCCCACTCCCCTCCAGAAAATGGATCCGATCAGGCTGTAAGCACCCGAAAACCCTTTCTTTCGCCGTCAATCGGGACCAGAAGGAAGCAAATAACAACGACGATGCAGCCTCCCTCTCCGATATTGATCAGTTTTTGTTTGAGAATTTCAGATCACTCTACTTGAGAGACGACGGCAATGACAACGATGAAAGAAGGGTTAAAGAAGATCGTCATGCTGAGGCTTTTTGGGATAAAACCTTACACCTGAAGGAACGTCGGCCGGACAATGAAACTGATCAAAACTCAGGAGGGGTTTTGTTCGAATCGCCTAGATTCAATATTGATCCGCCTGCCGTGGATCTTTGTGGCTCCCACAGGTTCTTTGTGTCCCCCGGCCGAGCATCAAGCTCGGTCATGGAGGAAGCTCGGACAAGTACTACCACTACATTCGAGGACTTGGGCTCAAGCTCCATCTCAACTCCAACCCTAAACGATTCGGCCACTACGTCGAACTCTAATAACAATGATGACGCGAAAGACGTCACGCTTCCCAACGACTGTATCGCGGTCTTGACCTACTCTCCGAGCCCATACGACGATTTCCAGCGATCCATGCATGAGGTGGTTGAAGCCCGCCTTCGACATCATGCAAAGGTGGATTGGAATTTCATGGAAGAGCTTCTGTTTTGTTATCTCAACCTCAACGAGAAGAAGTCTTACAGGTTCATATTAAGCGCCTTCGCTGATTTGGTCGTGGATTTGCGTCAGAATTCAGACAGAGTTTCGGAGACATCGTCGTCGTCTTCAAAATTTTGA

Protein Analysis

288

Amino Acids

32.8

Weight (kDa)

5.45

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 210 - 270 6.5e-20 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79960
fragaria_vesca FvH4_6g31210
malus_domestica MD17G1194900.v1.1
prunus_persica Prupe.3G070900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0137881
rosa_laevigata RLG00000019687
rosa_roxburghii Rroxscaffold_2G00106690
rosa_rugosa Rorug02G0347700
rosa_samantha Rh2AG394000 Rh2BG401400 Rh2CG380700 Rh2DG416200
rosa_wichuraiana Rw2G032250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 844
AatII GACGTC 1 cut(s) 591
AccB7I CCANNNNNTGG 1 cut(s) 87
AccII CGCG 2 cut(s) 581, 614
AciI CCGC 3 cut(s) 395, 614, 682
AclWI GGATC 6 cut(s) 61, 85, 98, 386, 414, 651
AcoI YGGCCR 3 cut(s) 326, 442, 546
AcsI RAATTY 4 cut(s) 211, 712, 817, 857
AcuI CTGAAG 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 588
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 2 cut(s) 87, 138
AgsI TTSAA 3 cut(s) 385, 677, 855
AluBI AGCT 4 cut(s) 456, 473, 516, 727
AluI AGCT 4 cut(s) 456, 473, 516, 727
Alw26I GTCTC 2 cut(s) 228, 830
AlwI GGATC 6 cut(s) 61, 85, 98, 386, 414, 651
AoxI GGCC 3 cut(s) 326, 442, 546
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 4 cut(s) 211, 712, 817, 857
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 1 cut(s) 142
AsuC2I CCSGG 1 cut(s) 441
AsuII TTCGAA 1 cut(s) 369
AvaII GGWCC 1 cut(s) 142
BaeI ACNNNNGTAYC 2 cut(s) 592, 625
BamHI GGATCC 1 cut(s) 90
BanII GRGCYC 2 cut(s) 512, 638
BarI GAAGNNNNNNTAC 2 cut(s) 749, 781
BbsI GAAGAC 1 cut(s) 843
BbvCI CCTCAGC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 185
BccI CCATC 1 cut(s) 527
BceAI ACGGC 3 cut(s) 116, 256, 386
BcgI CGANNNNNNTGC 2 cut(s) 438, 472
BclI TGATCA 2 cut(s) 193, 343
BcnI CCSGG 1 cut(s) 441
BcoDI GTCTC 2 cut(s) 228, 830
BfaI CTAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 786
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
BmcAI AGTACT 1 cut(s) 484
Bme1390I CCNGG 1 cut(s) 441
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 3 cut(s) 92, 143, 418
BmrFI CCNGG 1 cut(s) 441
BmsI GCATC 2 cut(s) 160, 458
BpiI GAAGAC 1 cut(s) 843
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 285
Bpu14I TTCGAA 1 cut(s) 369
BpuEI CTTGAG 2 cut(s) 250, 496
BpuMI CCSGG 1 cut(s) 441
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 1 cut(s) 588
BsaJI CCNNGG 2 cut(s) 402, 439
BsaXI ACNNNNNCTCC 6 cut(s) 23, 53, 613, 643, 827, 857
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 138
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 402, 439
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 87, 138
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 2 cut(s) 276, 366
BseX3I CGGCCG 2 cut(s) 326, 442
BseXI GCAGC 1 cut(s) 185
Bsh1236I CGCG 2 cut(s) 581, 614
Bsh1285I CGRYCG 2 cut(s) 329, 445
BshFI GGCC 3 cut(s) 328, 444, 548
BsiEI CGRYCG 2 cut(s) 329, 445
BsiSI CCGG 2 cut(s) 329, 441
BslFI GGGAC 2 cut(s) 155, 421
BslI CCNNNNNNNGG 2 cut(s) 87, 138
BsmAI GTCTC 2 cut(s) 228, 830
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 2 cut(s) 155, 421
BsnI GGCC 3 cut(s) 328, 444, 548
Bsp119I TTCGAA 1 cut(s) 369
Bsp1286I GDGCHC 2 cut(s) 512, 638
BspACI CCGC 3 cut(s) 395, 614, 682
BspANI GGCC 3 cut(s) 328, 444, 548
BspCNI CTCAG 2 cut(s) 277, 365
BspFNI CGCG 2 cut(s) 581, 614
BspLI GGNNCC 3 cut(s) 92, 143, 418
BspPI GGATC 6 cut(s) 61, 85, 98, 386, 414, 651
BspQI GCTCTTC 1 cut(s) 717
BspT104I TTCGAA 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 250
BssECI CCNNGG 2 cut(s) 402, 439
BssNI GRCGYC 1 cut(s) 588
Bst4CI ACNGT 1 cut(s) 608
Bst6I CTCTTC 1 cut(s) 717
BstACI GRCGYC 1 cut(s) 588
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 399, 682
BstDEI CTNAG 2 cut(s) 285, 352
BstDSI CCRYGG 1 cut(s) 402
BstFNI CGCG 2 cut(s) 581, 614
BstH2I RGCGCY 1 cut(s) 786
BstHHI GCGC 1 cut(s) 785
BstMAI GTCTC 2 cut(s) 228, 830
BstMCI CGRYCG 2 cut(s) 329, 445
BstMWI GCNNNNNNNGC 1 cut(s) 661
BstSCI CCNGG 1 cut(s) 439
BstUI CGCG 2 cut(s) 581, 614
BstV1I GCAGC 1 cut(s) 185
BstV2I GAAGAC 1 cut(s) 843
BstX2I RGATCY 3 cut(s) 66, 90, 406
BstYI RGATCY 3 cut(s) 66, 90, 406
BstZI CGGCCG 2 cut(s) 326, 442
BsuRI GGCC 3 cut(s) 328, 444, 548
BtgI CCRYGG 1 cut(s) 402
Cac8I GCNNGC 2 cut(s) 399, 682
CfoI GCGC 1 cut(s) 785
Cfr13I GGNCC 1 cut(s) 142
CseI GACGC 2 cut(s) 587, 800
Csp6I GTAC 1 cut(s) 483
CviAII CATG 6 cut(s) 281, 463, 661, 665, 695, 718
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 2 cut(s) 285, 352
DrdI GACNNNNNNGTC 1 cut(s) 844
DseDI GACNNNNNNGTC 1 cut(s) 844
EaeI YGGCCR 3 cut(s) 326, 442, 546
EagI CGGCCG 2 cut(s) 326, 442
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
EciI GGCGGA 1 cut(s) 384
EclXI CGGCCG 2 cut(s) 326, 442
Eco24I GRGCYC 2 cut(s) 512, 638
Eco47I GGWCC 1 cut(s) 142
Eco52I CGGCCG 2 cut(s) 326, 442
Eco57I CTGAAG 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 817
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 2 cut(s) 512, 638
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 6 cut(s) 284, 466, 664, 668, 698, 721
FaiI YATR 8 cut(s) 282, 464, 640, 662, 666, 696, 719, 776
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FaqI GGGAC 2 cut(s) 155, 421
FatI CATG 6 cut(s) 280, 462, 660, 664, 694, 717
FauI CCCGC 1 cut(s) 689
FbaI TGATCA 2 cut(s) 193, 343
Fnu4HI GCNGC 1 cut(s) 174
FriOI GRGCYC 2 cut(s) 512, 638
Fsp4HI GCNGC 1 cut(s) 174
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 784
GluI GCNGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 786
HaeIII GGCC 3 cut(s) 328, 444, 548
HapII CCGG 2 cut(s) 329, 441
HgaI GACGC 2 cut(s) 587, 800
HhaI GCGC 1 cut(s) 785
Hin1I GRCGYC 1 cut(s) 588
Hin1II CATG 6 cut(s) 284, 466, 664, 668, 698, 721
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HinfI GANTC 3 cut(s) 371, 381, 542
HpaII CCGG 2 cut(s) 329, 441
Hpy188I TCNGA 8 cut(s) 95, 187, 218, 477, 633, 816, 823, 835
Hpy188III TCNNGA 4 cut(s) 81, 140, 354, 619
Hpy99I CGWCG 7 cut(s) 170, 242, 327, 559, 647, 847, 850
HpyAV CCTTC 5 cut(s) 142, 255, 310, 695, 796
HpyCH4III ACNGT 1 cut(s) 608
HpyCH4IV ACGT 3 cut(s) 322, 554, 588
HpyCH4V TGCA 3 cut(s) 173, 664, 698
HpyF10VI GCNNNNNNNGC 1 cut(s) 661
HpyF3I CTNAG 2 cut(s) 285, 352
HpySE526I ACGT 3 cut(s) 322, 554, 588
Hsp92I GRCGYC 1 cut(s) 588
Hsp92II CATG 6 cut(s) 284, 466, 664, 668, 698, 721
HspAI GCGC 1 cut(s) 783
Ksp22I TGATCA 2 cut(s) 193, 343
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 2 cut(s) 422, 521
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 2 cut(s) 160, 458
MaeI CTAG 1 cut(s) 378
MaeII ACGT 3 cut(s) 322, 554, 588
MaeIII GTNAC 1 cut(s) 589
MboII GAAGA 4 cut(s) 284, 734, 769, 843
MflI RGATCY 3 cut(s) 66, 90, 406
MhlI GDGCHC 2 cut(s) 512, 638
MluCI AATT 4 cut(s) 211, 712, 817, 857
MmeI TCCRAC 1 cut(s) 554
Mph1103I ATGCAT 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 2 cut(s) 267, 779
MspI CCGG 2 cut(s) 329, 441
MspR9I CCNGG 1 cut(s) 441
MvnI CGCG 2 cut(s) 581, 614
MwoI GCNNNNNNNGC 1 cut(s) 661
NciI CCSGG 1 cut(s) 441
NlaIII CATG 6 cut(s) 284, 466, 664, 668, 698, 721
NlaIV GGNNCC 3 cut(s) 92, 143, 418
NmeAIII GCCGAG 1 cut(s) 470
NmuCI GTSAC 1 cut(s) 589
NsiI ATGCAT 1 cut(s) 666
NspV TTCGAA 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 717
PcsI WCGNNNNNNNCGW 3 cut(s) 551, 585, 839
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 3 cut(s) 371, 381, 542
PflFI GACNNNGTC 1 cut(s) 841
PflMI CCANNNNNTGG 1 cut(s) 87
PkrI GCNGC 1 cut(s) 175
PspN4I GGNNCC 3 cut(s) 92, 143, 418
PspPI GGNCC 1 cut(s) 142
PsuI RGATCY 3 cut(s) 66, 90, 406
PsyI GACNNNGTC 1 cut(s) 841
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 2 cut(s) 267, 779
SatI GCNGC 1 cut(s) 174
Sau96I GGNCC 1 cut(s) 142
ScaI AGTACT 1 cut(s) 484
ScrFI CCNGG 1 cut(s) 441
SduI GDGCHC 2 cut(s) 512, 638
SfaNI GCATC 2 cut(s) 160, 458
SfuI TTCGAA 1 cut(s) 369
SinI GGWCC 1 cut(s) 142
SmlI CTYRAG 2 cut(s) 229, 511
SmoI CTYRAG 2 cut(s) 229, 511
Sse9I AATT 4 cut(s) 211, 712, 817, 857
SsiI CCGC 3 cut(s) 395, 614, 682
SspI AATATT 1 cut(s) 388
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 439
TaaI ACNGT 1 cut(s) 608
TaiI ACGT 3 cut(s) 325, 557, 591
TaqI TCGA 4 cut(s) 369, 498, 557, 688
TaqII GACCGA 1 cut(s) 448
TasI AATT 4 cut(s) 211, 712, 817, 857
TatI WGTACW 1 cut(s) 482
TfiI GAWTC 3 cut(s) 371, 381, 542
Tru1I TTAA 2 cut(s) 267, 779
Tru9I TTAA 2 cut(s) 267, 779
TseFI GTSAC 1 cut(s) 589
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 4 cut(s) 270, 351, 706, 763
Tth111I GACNNNGTC 1 cut(s) 841
Van91I CCANNNNNTGG 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 142
XapI RAATTY 4 cut(s) 211, 712, 817, 857
XcmI CCANNNNNNNNNTGG 1 cut(s) 667
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 1 cut(s) 378
ZraI GACGTC 1 cut(s) 589
ZrmI AGTACT 1 cut(s) 484
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.