MD17G1201600.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0015272
Physical Location & Seq
Reverse (-)
24351438 .. 24355276
3839 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1201600.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGATGAAGAACCCAGACTTGGAAGAATCTATCAGTTTCCCGGGAAAACCCTCCCAATTCGCCGCCGAATGCTGGTTCGATGATGCATGCATACTCGACATGGACTACTTCGTCAAAACCCTAGCCGGAATTAAAGCCAAAGGTGTACGTCCTGACCTCATCGGTTCCATAATCACCCACTACGCATCCAAATGGCTCCCTGACCTCTCCGGCGACAGCAACATTTCCGACCAAAAGAACCTAACCAACTTCTCATCCTCTCCTGAAAGCATCACCGGCTTGTGGATGAGAAAACGCTTCTTCGTTGAAACCCTAGTGGGAGTCCTCCCTCCCGAGAAGGACTCCATCCCATGCAACTTCCTCCTCCGCTTGCTCCGCACCGCCAACATGGTGGCTGTGGAGCCCGCGTACCGGGCGGAGCTGGAGAAGAGGATATCATGGCAGCTGGACCAAGCCTCGCTGAAAGAGCTCATGATACCGTCTTTTAGTCACACATGCGGGACTTTGTTGGATGTTGAGCTTGTGACTAGGTTAGTTAAGAGGTTTGTGAACTTGGATGAGGCGGCTAAGAGCGGAGCCGCCATGGTGAAGGTGGCGAAGCTCGTGGATTGTTATCTCGCCGAGGCCGCCCTGGATGCGAATTTGAGCTTGAATGAGTTTGGAGCGCTTGCTGGGGCTCTGCCTAGCCATTCCCGTGCCACTGACGATGGCTTGTACCGAGCAATTGATACATATCTCAAAGCACATCCTGGAGTCTCCAAGCAAGATCGAAAGATTCTTTGTAGACTAATCGACAGTCGAAAACTCTCCCCAGAGGCATCTCTCCATGCAGCACAAAACGATCGCCTGCCTGTCCGAGCAGTAATCCAAGTCCTCTTTTCAGAGCAAAGCAAGCTTCACAGGAACATTGACTGGAGTGGCTCATTCAGCGGTAGCGCCAGAAGCCCGAACCCCTTTCTTGACCAGCCAGCGCGGTGCTTTTCGAAGCGTGAAATGAGTGCAATGCAGATGGAGATAAGGAACTTGAAGGAAGATGTGCTTAGGCTTCAAAACCAGTGCAACTCCATGCAAGTACAGGCAGAGAGGAAAGACAAAAAGAAAGGGTTATTTAAGTGGACTAGGAATTTGGGGATTTTGACTTCTTTTAGGGGTAATGTGGGCGTCGTGAAGATTAATGAGGGTGGAGATGGGGAGGTTGGGTATGGGAAACAAACGCCAATGGATATGAAGACCATGCTTGTTAGAGCTAGGGCTACTCCAAAGTGGAGGAAATCTGTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

428

Amino Acids

47.69

Weight (kDa)

9.18

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 23 - 274 1.3e-82 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52770 AT3G15570
fragaria_vesca FvH4_6g30650
malus_domestica MD17G1201600.v1.1
prunus_persica Prupe.3G077500_v2.0.a1
pyrus_communis pycom17g20550
rosa_chinensis RchiOBHm_Chr2g0137051
rosa_laevigata RLG00000019602
rosa_multiflora Rmu_sc0001374.1_g000031
rosa_roxburghii Rroxscaffold_2G00107740
rosa_rugosa Rorug02G0337600
rosa_samantha Rh2AG386500 Rh2BG393500 Rh2CG373800 Rh2DG409500
rosa_wichuraiana Rw2G031630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 784
AccII CGCG 2 cut(s) 407, 973
AcsI RAATTY 2 cut(s) 640, 1123
AcyI GRCGYC 1 cut(s) 1161
AfaI GTAC 4 cut(s) 147, 410, 716, 1074
AfeI AGCGCT 1 cut(s) 666
AfiI CCNNNNNNNGG 4 cut(s) 19, 72, 282, 411
AgsI TTSAA 4 cut(s) 308, 652, 1027, 1049
AjnI CCWGG 2 cut(s) 630, 748
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 8 cut(s) 421, 445, 469, 520, 601, 648, 895, 1247
AluI AGCT 8 cut(s) 421, 445, 469, 520, 601, 648, 895, 1247
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 1 cut(s) 760
Ama87I CYCGRG 2 cut(s) 40, 332
Aor51HI AGCGCT 1 cut(s) 666
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 2 cut(s) 442, 830
ApoI RAATTY 2 cut(s) 640, 1123
ArsI GACNNNNNNTTYG 2 cut(s) 1108, 1140
AseI ATTAAT 1 cut(s) 1173
AspLEI GCGC 3 cut(s) 667, 938, 973
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 3 cut(s) 41, 42, 413
AsuHPI GGTGA 3 cut(s) 166, 265, 598
AsuII TTCGAA 1 cut(s) 983
AvaI CYCGRG 2 cut(s) 40, 332
AvaII GGWCC 1 cut(s) 448
BanII GRGCYC 3 cut(s) 405, 471, 679
BauI CACGAG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 1235
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 454, 842
BccI CCATC 4 cut(s) 353, 701, 1003, 1181
BciT130I CCWGG 2 cut(s) 632, 750
BcnI CCSGG 3 cut(s) 41, 42, 413
BcoDI GTCTC 1 cut(s) 760
BfaI CTAG 6 cut(s) 122, 314, 528, 684, 1119, 1248
BfoI RGCGCY 2 cut(s) 668, 939
BisI GCNGC 6 cut(s) 63, 443, 564, 579, 627, 831
BlsI GCNGC 6 cut(s) 64, 444, 565, 580, 628, 832
Bme1390I CCNGG 5 cut(s) 41, 42, 413, 632, 750
Bme18I GGWCC 1 cut(s) 448
BmeT110I CYCGRG 2 cut(s) 40, 332
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 4 cut(s) 166, 197, 402, 577
BmrFI CCNGG 5 cut(s) 41, 42, 413, 632, 750
BmsI GCATC 5 cut(s) 73, 194, 279, 625, 827
BpiI GAAGAC 1 cut(s) 1235
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 3 cut(s) 443, 771, 934
Bpu10I CCTNAGC 1 cut(s) 1040
Bpu14I TTCGAA 1 cut(s) 983
BpuMI CCSGG 3 cut(s) 41, 42, 413
BsaBI GATNNNNATC 2 cut(s) 612, 732
BsaHI GRCGYC 1 cut(s) 1161
BsaJI CCNNGG 4 cut(s) 40, 582, 621, 630
BsaXI ACNNNNNCTCC 4 cut(s) 392, 422, 1184, 1214
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 72, 282, 411
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 917, 1054
Bse3DI GCAATG 1 cut(s) 1008
Bse8I GATNNNNATC 2 cut(s) 612, 732
BseBI CCWGG 2 cut(s) 632, 750
BseDI CCNNGG 4 cut(s) 40, 582, 621, 630
BseGI GGATG 8 cut(s) 185, 254, 291, 345, 517, 562, 640, 745
BseJI GATNNNNATC 2 cut(s) 612, 732
BseLI CCNNNNNNNGG 4 cut(s) 19, 72, 282, 411
BseMI GCAATG 1 cut(s) 1008
BseNI ACTGG 2 cut(s) 917, 1054
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 2 cut(s) 454, 842
BseYI CCCAGC 1 cut(s) 671
Bsh1236I CGCG 2 cut(s) 407, 973
Bsh1285I CGRYCG 1 cut(s) 844
BshFI GGCC 1 cut(s) 626
BsiEI CGRYCG 1 cut(s) 844
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 2 cut(s) 40, 332
BsiSI CCGG 5 cut(s) 41, 126, 210, 276, 412
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 4 cut(s) 19, 72, 282, 411
BsmAI GTCTC 1 cut(s) 760
BsmFI GGGAC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 1 cut(s) 626
BsoBI CYCGRG 2 cut(s) 40, 332
Bsp119I TTCGAA 1 cut(s) 983
Bsp1286I GDGCHC 3 cut(s) 405, 471, 679
Bsp143I GATC 2 cut(s) 766, 841
Bsp19I CCATGG 1 cut(s) 582
BspANI GGCC 1 cut(s) 626
BspFNI CGCG 2 cut(s) 407, 973
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 4 cut(s) 166, 197, 402, 577
BspT104I TTCGAA 1 cut(s) 983
BsrBI CCGCTC 1 cut(s) 573
BsrDI GCAATG 1 cut(s) 1008
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 2 cut(s) 917, 1054
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 4 cut(s) 40, 582, 621, 630
BssMI GATC 2 cut(s) 766, 841
BssNI GRCGYC 1 cut(s) 1161
BssSI CACGAG 1 cut(s) 602
BssT1I CCWWGG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 2 cut(s) 632, 750
Bst4CI ACNGT 2 cut(s) 480, 797
Bst6I CTCTTC 1 cut(s) 422
BstACI GRCGYC 1 cut(s) 1161
BstBI TTCGAA 1 cut(s) 983
BstC8I GCNNGC 7 cut(s) 88, 371, 405, 669, 848, 893, 969
BstDEI CTNAG 2 cut(s) 567, 1040
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 8 cut(s) 185, 254, 291, 345, 517, 562, 640, 745
BstFNI CGCG 2 cut(s) 407, 973
BstH2I RGCGCY 2 cut(s) 668, 939
BstHHI GCGC 3 cut(s) 667, 938, 973
BstKTI GATC 2 cut(s) 769, 844
BstMAI GTCTC 1 cut(s) 760
BstMBI GATC 2 cut(s) 766, 841
BstMCI CGRYCG 1 cut(s) 844
BstMWI GCNNNNNNNGC 9 cut(s) 276, 375, 413, 466, 626, 635, 892, 927, 942
BstNI CCWGG 2 cut(s) 632, 750
BstNSI RCATGY 2 cut(s) 90, 498
BstSCI CCNGG 5 cut(s) 39, 40, 411, 630, 748
BstUI CGCG 2 cut(s) 407, 973
BstV1I GCAGC 2 cut(s) 454, 842
BstV2I GAAGAC 1 cut(s) 1235
BstXI CCANNNNNNTGG 1 cut(s) 391
BsuRI GGCC 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 582
BtsCI GGATG 8 cut(s) 185, 254, 291, 345, 517, 562, 640, 745
BtsIMutI CAGTG 2 cut(s) 699, 1061
Cac8I GCNNGC 7 cut(s) 88, 371, 405, 669, 848, 893, 969
CciI TCATGA 1 cut(s) 471
CfoI GCGC 3 cut(s) 667, 938, 973
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 448
Cfr9I CCCGGG 1 cut(s) 40
CseI GACGC 1 cut(s) 1150
Csp6I GTAC 4 cut(s) 146, 409, 715, 1073
CviQI GTAC 4 cut(s) 146, 409, 715, 1073
DdeI CTNAG 2 cut(s) 567, 1040
DpnI GATC 2 cut(s) 768, 843
DpnII GATC 2 cut(s) 766, 841
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
EciI GGCGGA 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 582
Eco24I GRGCYC 3 cut(s) 405, 471, 679
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 448
Eco47III AGCGCT 1 cut(s) 666
Eco53kI GAGCTC 1 cut(s) 469
Eco88I CYCGRG 2 cut(s) 40, 332
EcoICRI GAGCTC 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 630, 748
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 582
EcoT22I ATGCAT 2 cut(s) 88, 92
EcoT38I GRGCYC 3 cut(s) 405, 471, 679
ErhI CCWWGG 1 cut(s) 582
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FaqI GGGAC 1 cut(s) 514
FauI CCCGC 2 cut(s) 412, 491
FblI GTMKAC 1 cut(s) 784
Fnu4HI GCNGC 6 cut(s) 63, 443, 564, 579, 627, 831
FokI GGATG 8 cut(s) 172, 241, 298, 332, 524, 569, 647, 732
FriOI GRGCYC 3 cut(s) 405, 471, 679
Fsp4HI GCNGC 6 cut(s) 63, 443, 564, 579, 627, 831
FspBI CTAG 6 cut(s) 122, 314, 528, 684, 1119, 1248
GlaI GCGC 3 cut(s) 666, 937, 972
GluI GCNGC 6 cut(s) 63, 443, 564, 579, 627, 831
GsaI CCCAGC 1 cut(s) 675
GsuI CTGGAG 3 cut(s) 443, 771, 934
HaeII RGCGCY 2 cut(s) 668, 939
HaeIII GGCC 1 cut(s) 626
HapII CCGG 5 cut(s) 41, 126, 210, 276, 412
HgaI GACGC 1 cut(s) 1150
HhaI GCGC 3 cut(s) 667, 938, 973
Hin1I GRCGYC 1 cut(s) 1161
Hin6I GCGC 3 cut(s) 665, 936, 971
HinP1I GCGC 3 cut(s) 665, 936, 971
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 5 cut(s) 26, 321, 341, 753, 775
HpaII CCGG 5 cut(s) 41, 126, 210, 276, 412
HphI GGTGA 3 cut(s) 166, 265, 598
Hpy166II GTNNAC 4 cut(s) 146, 550, 785, 1116
Hpy188I TCNGA 3 cut(s) 229, 857, 883
Hpy188III TCNNGA 7 cut(s) 152, 263, 332, 472, 959, 1165, 1281
Hpy8I GTNNAC 4 cut(s) 146, 550, 785, 1116
Hpy99I CGWCG 1 cut(s) 1166
HpyAV CCTTC 3 cut(s) 331, 583, 1021
HpyCH4III ACNGT 2 cut(s) 480, 797
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 8 cut(s) 86, 90, 354, 830, 1001, 1006, 1059, 1069
HpyF10VI GCNNNNNNNGC 9 cut(s) 276, 375, 413, 466, 626, 635, 892, 927, 942
HpyF3I CTNAG 2 cut(s) 567, 1040
HpySE526I ACGT 1 cut(s) 148
Hsp92I GRCGYC 1 cut(s) 1161
HspAI GCGC 3 cut(s) 665, 936, 971
Kzo9I GATC 2 cut(s) 766, 841
LmnI GCTCC 6 cut(s) 201, 378, 400, 418, 575, 662
Lsp1109I GCAGC 2 cut(s) 454, 842
LweI GCATC 5 cut(s) 73, 194, 279, 625, 827
MaeI CTAG 6 cut(s) 122, 314, 528, 684, 1119, 1248
MaeII ACGT 1 cut(s) 148
MaeIII GTNAC 2 cut(s) 488, 523
MalI GATC 2 cut(s) 768, 843
MbiI CCGCTC 1 cut(s) 573
MboI GATC 2 cut(s) 766, 841
MboII GAAGA 7 cut(s) 19, 35, 292, 439, 1043, 1180, 1240
MfeI CAATTG 1 cut(s) 723
MhlI GDGCHC 3 cut(s) 405, 471, 679
MluCI AATT 5 cut(s) 56, 129, 640, 723, 1123
MlyI GAGTC 3 cut(s) 330, 335, 762
MmeI TCCRAC 2 cut(s) 252, 489
Mph1103I ATGCAT 2 cut(s) 88, 92
MseI TTAA 4 cut(s) 132, 537, 1110, 1173
MslI CAYNNNNRTG 2 cut(s) 190, 693
MspA1I CMGCKG 2 cut(s) 445, 930
MspI CCGG 5 cut(s) 41, 126, 210, 276, 412
MspR9I CCNGG 5 cut(s) 41, 42, 413, 632, 750
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 1 cut(s) 74
MvaI CCWGG 2 cut(s) 632, 750
MvnI CGCG 2 cut(s) 407, 973
MwoI GCNNNNNNNGC 9 cut(s) 276, 375, 413, 466, 626, 635, 892, 927, 942
NciI CCSGG 3 cut(s) 41, 42, 413
NcoI CCATGG 1 cut(s) 582
NdeII GATC 2 cut(s) 766, 841
NlaIV GGNNCC 4 cut(s) 166, 197, 402, 577
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 2 cut(s) 488, 523
NsiI ATGCAT 2 cut(s) 88, 92
NspI RCATGY 2 cut(s) 90, 498
NspV TTCGAA 1 cut(s) 983
PaeI GCATGC 1 cut(s) 90
PagI TCATGA 1 cut(s) 471
PctI GAATGC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 26, 775
PfoI TCCNGGA 1 cut(s) 748
PkrI GCNGC 6 cut(s) 64, 444, 565, 580, 628, 832
Ple19I CGATCG 1 cut(s) 844
PleI GAGTC 3 cut(s) 329, 335, 761
PpsI GAGTC 3 cut(s) 329, 335, 761
PshBI ATTAAT 1 cut(s) 1173
Psp124BI GAGCTC 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 630, 748
PspFI CCCAGC 1 cut(s) 671
PspGI CCWGG 2 cut(s) 630, 748
PspN4I GGNNCC 4 cut(s) 166, 197, 402, 577
PspPI GGNCC 1 cut(s) 448
PvuI CGATCG 1 cut(s) 844
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 4 cut(s) 147, 410, 716, 1074
RsaNI GTAC 4 cut(s) 146, 409, 715, 1073
RseI CAYNNNNRTG 2 cut(s) 190, 693
SacI GAGCTC 1 cut(s) 471
SaqAI TTAA 4 cut(s) 132, 537, 1110, 1173
SatI GCNGC 6 cut(s) 63, 443, 564, 579, 627, 831
Sau3AI GATC 2 cut(s) 766, 841
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 3 cut(s) 330, 335, 762
ScrFI CCNGG 5 cut(s) 41, 42, 413, 632, 750
SduI GDGCHC 3 cut(s) 405, 471, 679
SfaNI GCATC 5 cut(s) 73, 194, 279, 625, 827
SfuI TTCGAA 1 cut(s) 983
SinI GGWCC 1 cut(s) 448
SmaI CCCGGG 1 cut(s) 42
SmiMI CAYNNNNRTG 2 cut(s) 190, 693
SphI GCATGC 1 cut(s) 90
Sse9I AATT 5 cut(s) 56, 129, 640, 723, 1123
SspMI CTAG 6 cut(s) 122, 314, 528, 684, 1119, 1248
SstI GAGCTC 1 cut(s) 471
StyD4I CCNGG 5 cut(s) 39, 40, 411, 630, 748
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 2 cut(s) 480, 797
TaiI ACGT 1 cut(s) 151
TaqI TCGA 6 cut(s) 78, 96, 769, 792, 799, 983
TasI AATT 5 cut(s) 56, 129, 640, 723, 1123
TatI WGTACW 1 cut(s) 1072
TauI GCSGC 4 cut(s) 65, 566, 581, 629
TfiI GAWTC 2 cut(s) 26, 775
Tru1I TTAA 4 cut(s) 132, 537, 1110, 1173
Tru9I TTAA 4 cut(s) 132, 537, 1110, 1173
TscAI CASTG 2 cut(s) 706, 1061
TseFI GTSAC 2 cut(s) 488, 523
TseI GCWGC 2 cut(s) 442, 830
Tsp45I GTSAC 2 cut(s) 488, 523
TspDTI ATGAA 2 cut(s) 20, 1241
TspMI CCCGGG 1 cut(s) 40
TspRI CASTG 2 cut(s) 706, 1061
VpaK11BI GGWCC 1 cut(s) 448
VspI ATTAAT 1 cut(s) 1173
XapI RAATTY 2 cut(s) 640, 1123
XceI RCATGY 2 cut(s) 90, 498
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmaI CCCGGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 784
XspI CTAG 6 cut(s) 122, 314, 528, 684, 1119, 1248
Zsp2I ATGCAT 2 cut(s) 88, 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.