Rorug02G0337600

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015272
Physical Location & Seq
Reverse (-)
41939918 .. 41941067
1150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0337600.1

Sequence Viewer

Length: 975 bp
ATGCTGGGGGTTTGGTCTTCTGAGGCCACTTCTCGCAATCTCCAAGATGCATCAATGTATGAGAGGTACGAGCAATGGATGGCCTATTATGGACGTGTTTATAATGACATGACCGAAAAGGAGGACCGCTTCAAAATATTCAAGGACAATGTGGCATTTATAGAATCTTCCAATAATGCTGGAAACAAACTTTACAAATTGAGTGTGAATCAATTTGCAGACCTTACAAATGAAGAATTCACTACCGCAAGAAATCGGTTCAAGGGGCATGAGTGTTCCACAATGACCACTACTTTCAAGTATGAAAATGCTAGCGTGCCAGCTACAATGGACTGGAGGCAGAAAGGAGCTGTAACTCCCATCAAGGACCAAGGCCAATGTGGATGTTGTTGGGCGTTCTCAGCCGTGGCAGCCATGGAAGGAATTACTCAACTTACAACTGGTAAATTGATTTCTTTGTCTGAGCAAGAGCTAGTTGACTGTGATGTCAATGGTGAAGATCAAGGCTGTGAGGGTGGCTTGATGGACGATGCCTTTCAGTTCATCAATCAAAATCATGGACTCAGTACCGAAGCAAATTATCCATACACTGGTGTTGATGGTACATGCAATGCCAAGAAGGAAGCAAACCATGCAGCCTCAATCACCGGCCACGAAGATGTGCCTGCAAATAGTGAAAGTGCACTCCTTAAGGCCGTCGCTAATCAACCTATTTCAGTTGCCATTGATGCTAGTGGATCCGATTTCCAGTTCTACTCAAGTGGTGTTTTCACAGGAACCTGTGGAACTAGCCTAGACCATGGTGTTACCGCTGTTGGTTATGGTGCTAGCAATGATGGGACTAAGTACTGGCTGGTGAAGAACTCATGGGGTGAGCAATGGGGTGAAGAAGGTTACATAAGGATGCAAAGAGATGTTGCTGCACCAGAAGGTCTCTGTGGTATTGCTATGGAAGCTTCCTATCCCACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

324

Amino Acids

35.44

Weight (kDa)

4.6

Isoelectric Point (pI)

21.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 23 - 80 4.4e-18 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 106 - 323 2.9e-85 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52770 AT3G15570
fragaria_vesca FvH4_6g30650
malus_domestica MD17G1201600.v1.1
prunus_persica Prupe.3G077500_v2.0.a1
pyrus_communis pycom17g20550
rosa_chinensis RchiOBHm_Chr2g0137051
rosa_laevigata RLG00000019602
rosa_multiflora Rmu_sc0001374.1_g000031
rosa_roxburghii Rroxscaffold_2G00107740
rosa_rugosa Rorug02G0337600
rosa_samantha Rh2AG386500 Rh2BG393500 Rh2CG373800 Rh2DG409500
rosa_wichuraiana Rw2G031630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AasI GACNNNNNNGTC 1 cut(s) 485
AccB7I CCANNNNNTGG 1 cut(s) 590
AciI CCGC 3 cut(s) 127, 246, 810
AclWI GGATC 2 cut(s) 732, 745
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 1 cut(s) 236
AfaI GTAC 4 cut(s) 68, 568, 604, 848
AfiI CCNNNNNNNGG 1 cut(s) 590
AflII CTTAAG 1 cut(s) 689
AflIII ACRYGT 1 cut(s) 94
AgsI TTSAA 4 cut(s) 133, 142, 262, 298
AjiI CACGTC 1 cut(s) 95
AluBI AGCT 4 cut(s) 323, 350, 472, 956
AluI AGCT 4 cut(s) 323, 350, 472, 956
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 1 cut(s) 938
Alw44I GTGCAC 1 cut(s) 681
AlwI GGATC 2 cut(s) 732, 745
AoxI GGCC 5 cut(s) 24, 81, 373, 649, 693
ApaLI GTGCAC 1 cut(s) 681
ApeKI GCWGC 3 cut(s) 410, 635, 920
ApoI RAATTY 1 cut(s) 236
AspS9I GGNCC 2 cut(s) 124, 367
AsuHPI GGTGA 5 cut(s) 506, 637, 868, 884, 896
AsuNHI GCTAGC 2 cut(s) 311, 827
AvaII GGWCC 2 cut(s) 124, 367
BaeGI GKGCMC 1 cut(s) 685
BamHI GGATCC 1 cut(s) 737
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 3 cut(s) 422, 647, 907
BccI CCATC 5 cut(s) 73, 368, 517, 593, 830
BceAI ACGGC 2 cut(s) 389, 680
BcoDI GTCTC 1 cut(s) 938
BfaI CTAG 6 cut(s) 312, 473, 732, 789, 794, 828
BfrI CTTAAG 1 cut(s) 689
BisI GCNGC 3 cut(s) 411, 636, 921
BlsI GCNGC 3 cut(s) 412, 637, 922
BmcAI AGTACT 1 cut(s) 848
Bme18I GGWCC 2 cut(s) 124, 367
BmgBI CACGTC 1 cut(s) 95
BmgT120I GGNCC 2 cut(s) 124, 367
BmiI GGNNCC 2 cut(s) 739, 778
BmsI GCATC 5 cut(s) 37, 59, 520, 718, 894
BmtI GCTAGC 2 cut(s) 315, 831
BpiI GAAGAC 1 cut(s) 9
BpmI CTGGAG 1 cut(s) 355
BpuEI CTTGAG 1 cut(s) 742
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 4 cut(s) 370, 405, 414, 799
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 5 cut(s) 338, 445, 595, 748, 854
Bse3DI GCAATG 4 cut(s) 80, 616, 838, 884
BseDI CCNNGG 4 cut(s) 370, 405, 414, 799
BseGI GGATG 3 cut(s) 84, 389, 909
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMI GCAATG 4 cut(s) 80, 616, 838, 884
BseMII CTCAG 4 cut(s) 12, 414, 453, 577
BseNI ACTGG 5 cut(s) 338, 445, 595, 748, 854
BseSI GKGCMC 1 cut(s) 685
BseXI GCAGC 3 cut(s) 422, 647, 907
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 1 cut(s) 906
BshFI GGCC 5 cut(s) 26, 83, 375, 651, 695
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 1 cut(s) 648
BslFI GGGAC 1 cut(s) 853
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 1 cut(s) 938
BsmFI GGGAC 1 cut(s) 853
BsnI GGCC 5 cut(s) 26, 83, 375, 651, 695
Bso31I GGTCTC 1 cut(s) 938
Bsp1286I GDGCHC 1 cut(s) 685
Bsp143I GATC 2 cut(s) 499, 737
Bsp19I CCATGG 2 cut(s) 414, 799
BspACI CCGC 3 cut(s) 127, 246, 810
BspANI GGCC 5 cut(s) 26, 83, 375, 651, 695
BspCNI CTCAG 4 cut(s) 13, 413, 454, 576
BspLI GGNNCC 2 cut(s) 739, 778
BspOI GCTAGC 2 cut(s) 315, 831
BspPI GGATC 2 cut(s) 732, 745
BspTI CTTAAG 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 938
BsrDI GCAATG 4 cut(s) 80, 616, 838, 884
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 5 cut(s) 338, 445, 595, 748, 854
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 4 cut(s) 370, 405, 414, 799
BssMI GATC 2 cut(s) 499, 737
BssT1I CCWWGG 3 cut(s) 370, 414, 799
Bst4CI ACNGT 1 cut(s) 482
BstAFI CTTAAG 1 cut(s) 689
BstAPI GCANNNNNTGC 1 cut(s) 632
BstC8I GCNNGC 5 cut(s) 313, 317, 321, 666, 829
BstDEI CTNAG 5 cut(s) 21, 400, 462, 563, 843
BstDSI CCRYGG 3 cut(s) 405, 414, 799
BstF5I GGATG 3 cut(s) 84, 389, 909
BstKTI GATC 2 cut(s) 502, 740
BstMAI GTCTC 1 cut(s) 938
BstMBI GATC 2 cut(s) 499, 737
BstMWI GCNNNNNNNGC 5 cut(s) 401, 410, 632, 728, 953
BstNSI RCATGY 1 cut(s) 609
BstSLI GKGCMC 1 cut(s) 685
BstV1I GCAGC 3 cut(s) 422, 647, 907
BstV2I GAAGAC 1 cut(s) 9
BstX2I RGATCY 1 cut(s) 737
BstYI RGATCY 1 cut(s) 737
BsuRI GGCC 5 cut(s) 26, 83, 375, 651, 695
BtgI CCRYGG 3 cut(s) 405, 414, 799
BtrI CACGTC 1 cut(s) 95
BtsCI GGATG 3 cut(s) 84, 389, 909
BtsI GCAGTG 1 cut(s) 966
BtsIMutI CAGTG 2 cut(s) 588, 966
Cac8I GCNNGC 5 cut(s) 313, 317, 321, 666, 829
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 2 cut(s) 124, 367
Csp6I GTAC 4 cut(s) 67, 567, 603, 847
CviAII CATG 8 cut(s) 109, 269, 415, 557, 606, 632, 800, 867
CviQI GTAC 4 cut(s) 67, 567, 603, 847
DdeI CTNAG 5 cut(s) 21, 400, 462, 563, 843
DpnI GATC 2 cut(s) 501, 739
DpnII GATC 2 cut(s) 499, 737
DrdI GACNNNNNNGTC 1 cut(s) 485
DseDI GACNNNNNNGTC 1 cut(s) 485
EaeI YGGCCR 1 cut(s) 649
Eco130I CCWWGG 3 cut(s) 370, 414, 799
Eco31I GGTCTC 1 cut(s) 938
Eco47I GGWCC 2 cut(s) 124, 367
EcoRI GAATTC 1 cut(s) 236
EcoT14I CCWWGG 3 cut(s) 370, 414, 799
EcoT22I ATGCAT 1 cut(s) 52
ErhI CCWWGG 3 cut(s) 370, 414, 799
FaeI CATG 8 cut(s) 112, 272, 418, 560, 609, 635, 803, 870
FaqI GGGAC 1 cut(s) 853
FatI CATG 8 cut(s) 108, 268, 414, 556, 605, 631, 799, 866
Fnu4HI GCNGC 3 cut(s) 411, 636, 921
FokI GGATG 3 cut(s) 91, 396, 916
Fsp4HI GCNGC 3 cut(s) 411, 636, 921
FspBI CTAG 6 cut(s) 312, 473, 732, 789, 794, 828
GluI GCNGC 3 cut(s) 411, 636, 921
GsaI CCCAGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 355
HaeIII GGCC 5 cut(s) 26, 83, 375, 651, 695
HapII CCGG 1 cut(s) 648
Hin1II CATG 8 cut(s) 112, 272, 418, 560, 609, 635, 803, 870
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 954
HinfI GANTC 3 cut(s) 164, 208, 561
HpaII CCGG 1 cut(s) 648
HphI GGTGA 5 cut(s) 506, 637, 868, 884, 896
Hpy166II GTNNAC 2 cut(s) 478, 683
Hpy188I TCNGA 3 cut(s) 22, 463, 742
Hpy8I GTNNAC 2 cut(s) 478, 683
Hpy99I CGWCG 1 cut(s) 701
HpyAV CCTTC 4 cut(s) 413, 613, 884, 923
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 9 cut(s) 50, 218, 609, 635, 668, 683, 907, 923, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 401, 410, 632, 728, 953
HpyF3I CTNAG 5 cut(s) 21, 400, 462, 563, 843
HpySE526I ACGT 1 cut(s) 94
Hsp92II CATG 8 cut(s) 112, 272, 418, 560, 609, 635, 803, 870
Kzo9I GATC 2 cut(s) 499, 737
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 3 cut(s) 422, 647, 907
LweI GCATC 5 cut(s) 37, 59, 520, 718, 894
MaeI CTAG 6 cut(s) 312, 473, 732, 789, 794, 828
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 3 cut(s) 352, 805, 893
MalI GATC 2 cut(s) 501, 739
MboI GATC 2 cut(s) 499, 737
MboII GAAGA 7 cut(s) 9, 159, 245, 509, 668, 871, 899
MflI RGATCY 1 cut(s) 737
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 6 cut(s) 197, 212, 236, 423, 446, 577
MlyI GAGTC 1 cut(s) 555
MnlI CCTC 6 cut(s) 16, 57, 115, 330, 505, 649
Mph1103I ATGCAT 1 cut(s) 52
MseI TTAA 1 cut(s) 690
MslI CAYNNNNRTG 2 cut(s) 657, 902
MspA1I CMGCKG 1 cut(s) 812
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 1 cut(s) 648
MwoI GCNNNNNNNGC 5 cut(s) 401, 410, 632, 728, 953
NcoI CCATGG 2 cut(s) 414, 799
NdeII GATC 2 cut(s) 499, 737
NheI GCTAGC 2 cut(s) 311, 827
NlaIII CATG 8 cut(s) 112, 272, 418, 560, 609, 635, 803, 870
NlaIV GGNNCC 2 cut(s) 739, 778
NsiI ATGCAT 1 cut(s) 52
NspI RCATGY 1 cut(s) 609
PfeI GAWTC 2 cut(s) 164, 208
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 3 cut(s) 412, 637, 922
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
PsiI TTATAA 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 4
PspN4I GGNNCC 2 cut(s) 739, 778
PspPI GGNCC 2 cut(s) 124, 367
PsuI RGATCY 1 cut(s) 737
RsaI GTAC 4 cut(s) 68, 568, 604, 848
RsaNI GTAC 4 cut(s) 67, 567, 603, 847
RseI CAYNNNNRTG 2 cut(s) 657, 902
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 3 cut(s) 411, 636, 921
Sau3AI GATC 2 cut(s) 499, 737
Sau96I GGNCC 2 cut(s) 124, 367
ScaI AGTACT 1 cut(s) 848
SchI GAGTC 1 cut(s) 555
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 5 cut(s) 37, 59, 520, 718, 894
SinI GGWCC 2 cut(s) 124, 367
SmiMI CAYNNNNRTG 2 cut(s) 657, 902
SmlI CTYRAG 2 cut(s) 689, 757
SmoI CTYRAG 2 cut(s) 689, 757
Sse9I AATT 6 cut(s) 197, 212, 236, 423, 446, 577
SsiI CCGC 3 cut(s) 127, 246, 810
SspI AATATT 1 cut(s) 138
SspMI CTAG 6 cut(s) 312, 473, 732, 789, 794, 828
StyI CCWWGG 3 cut(s) 370, 414, 799
TaaI ACNGT 1 cut(s) 482
TaiI ACGT 1 cut(s) 97
TaqII GACCGA 1 cut(s) 128
TasI AATT 6 cut(s) 197, 212, 236, 423, 446, 577
TatI WGTACW 1 cut(s) 846
TfiI GAWTC 2 cut(s) 164, 208
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 2 cut(s) 595, 973
TseI GCWGC 3 cut(s) 410, 635, 920
TspDTI ATGAA 3 cut(s) 246, 318, 532
TspRI CASTG 2 cut(s) 595, 973
Van91I CCANNNNNTGG 1 cut(s) 590
Vha464I CTTAAG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 681
VpaK11BI GGWCC 2 cut(s) 124, 367
XapI RAATTY 1 cut(s) 236
XceI RCATGY 1 cut(s) 609
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XspI CTAG 6 cut(s) 312, 473, 732, 789, 794, 828
ZrmI AGTACT 1 cut(s) 848
Zsp2I ATGCAT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.