Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012715

Physical Location & Seq

Genomic Coordinates Forward (+)

27054841 .. 27056405

Gene Structure 1565 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1222200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 825 bp

ATGCCGCTCTTACCGAAAATCGATTCGATTCATATTCGTGAGGTGTGGAACGACAATCTGGAGGAGGAATTTGCTTTGATACGCAAAATTGTCGATGATTATCCGTATGTCGCCATGGACACCGAGTTTCCGGGCATTGTTATTCGTCCGGTGGGCAATTTCAAGAACGGTTACGACTACCATTACCAGACCTTGAAGGACAACGTGGATATGCTCAAGCTGATTCAACTCGGCCTCACGTTTTCCGATGAGCAAGGGAATTTCCCCTCTTGTGGAACTGATAAACAATGCATTTGGCAGTTCAATTTTCGCGAGTTCAATCTCAATGAGGATGTATTCTCCAGTGATTCTATTGAGCTTCTGCGCCAAAGCGGCATCGATTTTAAGAAGAACAACGAAAAGGGTATTGATGCAAAGCGGTTTGGGGAGCTCCTAATGTCGTCTGGGATTGTGTTGAATGATAACGTGCATTGGGTCACCTTCCATAGCGGCTATGATTTTGGTTACTTGCTAAAGCTTTTGACCTGCCAGAACTCGCCCCAAACTCAAGCAGGGTTCTTTAATTTGATCAACATATACTTTCCGAAAATATACGATATCAAGCATCTGATGAAGTTTTGCAATAGCCTCCATGGCGGGTTGAACAAGCTTGCGGAACTGTTGGAAGTGGAGAGAGTTGGCATTTGTCACCAGGCAGGTTCGGATAGTTTGCTCACGTCCTGTACGTTCAGAAAACTGAAGGAGAGTTTCTTTAGCGGTTCGATGGAGAAGTATGCTGGTGTCTTGTATGGTTTAGGTGTGGATAATGGACAGGATACTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000288 GO:0000291 GO:0000932 GO:0000956 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004535 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0017148 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1990904 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

CAF1

Protein Analysis

275

Amino Acids

31.37

Weight (kDa)

5.17

Isoelectric Point (pI)

35.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1	PF04857	15 - 136	2e-13	CAF1 family ribonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012715)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80780 AT1G80780 AT1G80780 AT2G32070
fragaria_vesca	FvH4_6g28750
malus_domestica	MD17G1222200.v1.1
prunus_persica	Prupe.3G097100_v2.0.a1
pyrus_communis	pycom09g14370 pycom17g22590
rosa_chinensis	RchiOBHm_Chr2g0133151
rosa_laevigata	RLG00000019312
rosa_roxburghii	Rroxscaffold_2G00111360
rosa_rugosa	Rorug02G0310300
rosa_samantha	Rh2AG362600 Rh2BG368200 Rh2CG345800 Rh2DG385500
rosa_wichuraiana	Rw2G029480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	533, 686
AccBSI	CCGCTC	1 cut(s)	7
AccII	CGCG	1 cut(s)	312
AciI	CCGC	7 cut(s)	5, 372, 418, 489, 636, 653, 756
AcsI	RAATTY	2 cut(s)	68, 259
AcuI	CTGAAG	1 cut(s)	758
AfaI	GTAC	1 cut(s)	724
AfiI	CCNNNNNNNGG	1 cut(s)	272
AgsI	TTSAA	7 cut(s)	163, 196, 227, 304, 319, 457, 643
AjiI	CACGTC	1 cut(s)	717
AjnI	CCWGG	1 cut(s)	690
AluBI	AGCT	5 cut(s)	220, 358, 430, 517, 649
AluI	AGCT	5 cut(s)	220, 358, 430, 517, 649
Alw21I	GWGCWC	1 cut(s)	432
AoxI	GGCC	1 cut(s)	232
ApoI	RAATTY	2 cut(s)	68, 259
AspLEI	GCGC	1 cut(s)	366
AsuC2I	CCSGG	1 cut(s)	132
AsuHPI	GGTGA	2 cut(s)	469, 680
BanII	GRGCYC	1 cut(s)	432
Bbv12I	GWGCWC	1 cut(s)	432
BccI	CCATC	1 cut(s)	757
BcgI	CGANNNNNNTGC	2 cut(s)	73, 107
BciT130I	CCWGG	1 cut(s)	692
BciVI	GTATCC	1 cut(s)	808
BclI	TGATCA	1 cut(s)	567
BcnI	CCSGG	1 cut(s)	132
BfuAI	ACCTGC	2 cut(s)	533, 686
BfuI	GTATCC	1 cut(s)	808
BglI	GCCNNNNNGGC	2 cut(s)	372, 633
BisI	GCNGC	3 cut(s)	5, 373, 490
BlsI	GCNGC	3 cut(s)	6, 374, 491
Bme1390I	CCNGG	2 cut(s)	132, 692
BmgBI	CACGTC	1 cut(s)	717
BmrFI	CCNGG	2 cut(s)	132, 692
BmsI	GCATC	3 cut(s)	384, 400, 613
BpmI	CTGGAG	2 cut(s)	80, 325
BpuEI	CTTGAG	2 cut(s)	200, 531
BpuMI	CCSGG	1 cut(s)	132
Bsa29I	ATCGAT	2 cut(s)	21, 378
BsaBI	GATNNNNATC	1 cut(s)	99
BsaJI	CCNNGG	2 cut(s)	114, 631
BsaWI	WCCGGW	1 cut(s)	148
Bsc4I	CCNNNNNNNGG	1 cut(s)	272
Bse1I	ACTGG	1 cut(s)	342
Bse8I	GATNNNNATC	1 cut(s)	99
BseBI	CCWGG	1 cut(s)	692
BseCI	ATCGAT	2 cut(s)	21, 378
BseDI	CCNNGG	2 cut(s)	114, 631
BseGI	GGATG	1 cut(s)	337
BseJI	GATNNNNATC	1 cut(s)	99
BseLI	CCNNNNNNNGG	1 cut(s)	272
BseNI	ACTGG	1 cut(s)	342
BseRI	GAGGAG	1 cut(s)	77
Bsh1236I	CGCG	1 cut(s)	312
BshFI	GGCC	1 cut(s)	234
BshVI	ATCGAT	2 cut(s)	21, 378
BsiHKAI	GWGCWC	1 cut(s)	432
BsiSI	CCGG	2 cut(s)	131, 149
BslI	CCNNNNNNNGG	1 cut(s)	272
BsnI	GGCC	1 cut(s)	234
Bsp1286I	GDGCHC	1 cut(s)	432
Bsp143I	GATC	1 cut(s)	567
Bsp19I	CCATGG	2 cut(s)	114, 631
Bsp68I	TCGCGA	1 cut(s)	312
BspACI	CCGC	7 cut(s)	5, 372, 418, 489, 636, 653, 756
BspANI	GGCC	1 cut(s)	234
BspDI	ATCGAT	2 cut(s)	21, 378
BspFNI	CGCG	1 cut(s)	312
BspMI	ACCTGC	2 cut(s)	533, 686
BsrBI	CCGCTC	1 cut(s)	7
BsrI	ACTGG	1 cut(s)	342
BssECI	CCNNGG	2 cut(s)	114, 631
BssMI	GATC	1 cut(s)	567
BssT1I	CCWWGG	2 cut(s)	114, 631
Bst2UI	CCWGG	1 cut(s)	692
Bst4CI	ACNGT	2 cut(s)	170, 660
BstC8I	GCNNGC	1 cut(s)	651
BstDSI	CCRYGG	2 cut(s)	114, 631
BstEII	GGTNACC	1 cut(s)	475
BstF5I	GGATG	1 cut(s)	337
BstFNI	CGCG	1 cut(s)	312
BstHHI	GCGC	1 cut(s)	366
BstKTI	GATC	1 cut(s)	570
BstMBI	GATC	1 cut(s)	567
BstMWI	GCNNNNNNNGC	2 cut(s)	372, 633
BstNI	CCWGG	1 cut(s)	692
BstPI	GGTNACC	1 cut(s)	475
BstSCI	CCNGG	2 cut(s)	130, 690
BstUI	CGCG	1 cut(s)	312
Bsu15I	ATCGAT	2 cut(s)	21, 378
BsuI	GTATCC	1 cut(s)	808
BsuRI	GGCC	1 cut(s)	234
BsuTUI	ATCGAT	2 cut(s)	21, 378
BtgI	CCRYGG	2 cut(s)	114, 631
BtrI	CACGTC	1 cut(s)	717
BtsCI	GGATG	1 cut(s)	337
BtsIMutI	CAGTG	1 cut(s)	349
BtuMI	TCGCGA	1 cut(s)	312
BveI	ACCTGC	2 cut(s)	533, 686
Cac8I	GCNNGC	1 cut(s)	651
CfoI	GCGC	1 cut(s)	366
ClaI	ATCGAT	2 cut(s)	21, 378
Csp6I	GTAC	1 cut(s)	723
CviAII	CATG	2 cut(s)	115, 632
CviJI	RGCY	8 cut(s)	220, 234, 358, 430, 492, 517, 627, 649
CviKI_1	RGCY	8 cut(s)	220, 234, 358, 430, 492, 517, 627, 649
CviQI	GTAC	1 cut(s)	723
DpnI	GATC	1 cut(s)	569
DpnII	GATC	1 cut(s)	567
Ecl136II	GAGCTC	1 cut(s)	430
Eco130I	CCWWGG	2 cut(s)	114, 631
Eco24I	GRGCYC	1 cut(s)	432
Eco32I	GATATC	1 cut(s)	598
Eco53kI	GAGCTC	1 cut(s)	430
Eco57I	CTGAAG	1 cut(s)	758
Eco91I	GGTNACC	1 cut(s)	475
EcoICRI	GAGCTC	1 cut(s)	430
EcoO65I	GGTNACC	1 cut(s)	475
EcoRII	CCWGG	1 cut(s)	690
EcoRV	GATATC	1 cut(s)	598
EcoT14I	CCWWGG	2 cut(s)	114, 631
EcoT22I	ATGCAT	1 cut(s)	293
EcoT38I	GRGCYC	1 cut(s)	432
ErhI	CCWWGG	2 cut(s)	114, 631
FaeI	CATG	2 cut(s)	118, 635
FatI	CATG	2 cut(s)	114, 631
FauI	CCCGC	1 cut(s)	629
FbaI	TGATCA	1 cut(s)	567
Fnu4HI	GCNGC	3 cut(s)	5, 373, 490
FokI	GGATG	1 cut(s)	344
FriOI	GRGCYC	1 cut(s)	432
Fsp4HI	GCNGC	3 cut(s)	5, 373, 490
GlaI	GCGC	1 cut(s)	365
GluI	GCNGC	3 cut(s)	5, 373, 490
GsuI	CTGGAG	2 cut(s)	80, 325
HaeIII	GGCC	1 cut(s)	234
HapII	CCGG	2 cut(s)	131, 149
HhaI	GCGC	1 cut(s)	366
Hin1II	CATG	2 cut(s)	118, 635
Hin6I	GCGC	1 cut(s)	364
HinP1I	GCGC	1 cut(s)	364
HindIII	AAGCTT	2 cut(s)	515, 647
HinfI	GANTC	4 cut(s)	23, 28, 223, 347
HpaII	CCGG	2 cut(s)	131, 149
HphI	GGTGA	2 cut(s)	469, 680
Hpy188I	TCNGA	6 cut(s)	247, 585, 609, 703, 731, 824
Hpy188III	TCNNGA	4 cut(s)	38, 59, 163, 311
HpyAV	CCTTC	3 cut(s)	190, 490, 733
HpyCH4III	ACNGT	2 cut(s)	170, 660
HpyCH4IV	ACGT	5 cut(s)	204, 239, 465, 716, 725
HpyCH4V	TGCA	4 cut(s)	291, 413, 469, 621
HpyF10VI	GCNNNNNNNGC	2 cut(s)	372, 633
HpySE526I	ACGT	5 cut(s)	204, 239, 465, 716, 725
Hsp92II	CATG	2 cut(s)	118, 635
HspAI	GCGC	1 cut(s)	364
Ksp22I	TGATCA	1 cut(s)	567
Kzo9I	GATC	1 cut(s)	567
LmnI	GCTCC	2 cut(s)	427, 435
LweI	GCATC	3 cut(s)	384, 400, 613
MaeII	ACGT	5 cut(s)	204, 239, 465, 716, 725
MaeIII	GTNAC	4 cut(s)	170, 475, 503, 686
MalI	GATC	1 cut(s)	569
MbiI	CCGCTC	1 cut(s)	7
MboI	GATC	1 cut(s)	567
MboII	GAAGA	1 cut(s)	400
MhlI	GDGCHC	1 cut(s)	432
MluCI	AATT	6 cut(s)	68, 87, 157, 259, 304, 562
MmeI	TCCRAC	1 cut(s)	642
MnlI	CCTC	7 cut(s)	34, 55, 58, 245, 277, 322, 638
Mph1103I	ATGCAT	1 cut(s)	293
MseI	TTAA	2 cut(s)	384, 561
MslI	CAYNNNNRTG	1 cut(s)	36
MspI	CCGG	2 cut(s)	131, 149
MspR9I	CCNGG	2 cut(s)	132, 692
MvaI	CCWGG	1 cut(s)	692
MvnI	CGCG	1 cut(s)	312
MwoI	GCNNNNNNNGC	2 cut(s)	372, 633
NciI	CCSGG	1 cut(s)	132
NcoI	CCATGG	2 cut(s)	114, 631
NdeII	GATC	1 cut(s)	567
NlaIII	CATG	2 cut(s)	118, 635
NmeAIII	GCCGAG	1 cut(s)	210
NmuCI	GTSAC	2 cut(s)	475, 686
NruI	TCGCGA	1 cut(s)	312
NsiI	ATGCAT	1 cut(s)	293
PcsI	WCGNNNNNNNCGW	1 cut(s)	722
PfeI	GAWTC	4 cut(s)	23, 28, 223, 347
PkrI	GCNGC	3 cut(s)	6, 374, 491
Psp124BI	GAGCTC	1 cut(s)	432
Psp6I	CCWGG	1 cut(s)	690
PspEI	GGTNACC	1 cut(s)	475
PspGI	CCWGG	1 cut(s)	690
RruI	TCGCGA	1 cut(s)	312
RsaI	GTAC	1 cut(s)	724
RsaNI	GTAC	1 cut(s)	723
RseI	CAYNNNNRTG	1 cut(s)	36
SacI	GAGCTC	1 cut(s)	432
SaqAI	TTAA	2 cut(s)	384, 561
SatI	GCNGC	3 cut(s)	5, 373, 490
Sau3AI	GATC	1 cut(s)	567
ScrFI	CCNGG	2 cut(s)	132, 692
SduI	GDGCHC	1 cut(s)	432
SfaNI	GCATC	3 cut(s)	384, 400, 613
SmiMI	CAYNNNNRTG	1 cut(s)	36
SmlI	CTYRAG	2 cut(s)	215, 546
SmoI	CTYRAG	2 cut(s)	215, 546
Sse9I	AATT	6 cut(s)	68, 87, 157, 259, 304, 562
SsiI	CCGC	7 cut(s)	5, 372, 418, 489, 636, 653, 756
SstI	GAGCTC	1 cut(s)	432
StyD4I	CCNGG	2 cut(s)	130, 690
StyI	CCWWGG	2 cut(s)	114, 631
TaaI	ACNGT	2 cut(s)	170, 660
TaiI	ACGT	5 cut(s)	207, 242, 468, 719, 728
TaqI	TCGA	5 cut(s)	21, 26, 93, 378, 761
TasI	AATT	6 cut(s)	68, 87, 157, 259, 304, 562
TauI	GCSGC	3 cut(s)	7, 375, 492
TfiI	GAWTC	4 cut(s)	23, 28, 223, 347
Tru1I	TTAA	2 cut(s)	384, 561
Tru9I	TTAA	2 cut(s)	384, 561
TscAI	CASTG	1 cut(s)	349
TseFI	GTSAC	2 cut(s)	475, 686
Tsp45I	GTSAC	2 cut(s)	475, 686
TspDTI	ATGAA	2 cut(s)	20, 626
TspGWI	ACGGA	1 cut(s)	93
TspRI	CASTG	1 cut(s)	349
XapI	RAATTY	2 cut(s)	68, 259
Zsp2I	ATGCAT	1 cut(s)	293

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.