MD17G1224300.v1.1

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0021196
Physical Location & Seq
Reverse (-)
27280832 .. 27283729
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1224300.v1.1.491

Sequence Viewer

Length: 2898 bp
ATGGCTGATGCAGTCGCCTTGGTCTCCAGACTCATAGACCAGCTCCAAGAAAAACACAGGTTCCCAGAGCTCGCCAGAATCCTCCGCGACATCGAACCTGTTCTAGAGGACGCCGAGAAGAGGGGAGTGAAGGAGGAGCCAGTCAGAGTGTGGTTGGACAAGCTCAAAGACGCCAGCTGCGACATCGAGGACGTGCTCGATGAGTGCTGCAGCGCTGCCGTCCCTGAATCAGAAGATACCCAGAAGGAGGAAGAAGAAGAAAGTCGTGGAATATCGAAAGCTTTTAGCTTTGTGAAGGTATGCTTTTCCCAACTCACCTCGTGCTTTTGTTGCACTGATATAGTCGATTGGGTTCGAATTGCAAAGAGGATAAAGTGTTTGAGGGAAAGACTGAGTGAAATTGCAACTGAGAGACATAGGTTCAACTTTAATTCTGTAAAAAATAGAGAGATTGAACAATCCGGGAGACTAGGAATTGGAACTGCCACTTATAAGTTAATTGAAGAAGTAGAGTCCGCAATCTATGGTCGAGACGAAGATACGAAGATTTTGATAAGCAAATTGGTACCTGAGCGGAGTGAAGGGTGTTGGGGGAATGGTGTAATCTCAATTGTTGGGGTGGGTGGAATGGGGAAAACCACTCTTGCCCGGTTAGCATATAATGATCAAAGTGTCAGAACCCATTTCGATCTGAGGATATGGGGTTGCGACTCTGTTTCGCCTGATGAGCCGAGGGTTGCCAGAGAAATTCTTGAAGCTATCCCTCGTAAGAGTGGAACGTTGAACTTTGTTGAACGGGAAGCTTTGTTAGAGAGTATTCGTGCATCGATTGGTGGAAAGAAGTTCCTTCTAGTCCTTGATAATGCGTGGGATCTCTGGGATGGAGACTTCTCAAAGGGGTATGTTCTTTGGAAGCTTTTGCGTTTTGGTGCAGCAGGGAGTAGGATTTTGCTGACTGCAAGATCTAGCAACATTGCTAAGATGATGGGAGCTACTAGCGTAATTGATCTAAGCGCAGTGTCGCAGGAAGATGCGTGGTTAATGTTTAGACAGAGAGCTTTTTTCGGAAGGGATGCAAGTGAGTGCAAACAACTCGAAAGAATTGGTAAGAAAATTGTAAAGAAGTGCTATGGCTTGCCTCTTGCGATAAAGACCTTGGCGGGTCTCATGCACTTTAAGAATACCAGAGGACAGTGGGAATCTGTTTTGCGAGAAATGGTGTGGGAATTGGGACATGCCAACAACATAAGCACTTTTGACCTGCTTCTGTTTAGCTATCATGATCTGCCACCTGTGCTTAGATGTTGTTTCTCATATTGTGCAATATTTCCGAAAGGTCATGTCATTGAGAGAGACAATTTGATCAAAATGTGGATGTCGCAAGGTTATCTTGGTTTCAATGTCGACAGAAATAAAGAGAAGGAAATACTAGGCCAAGAATGCTTTATGAATTTAGCGATGCGGTCTTTCTTCCAAGACTTTGAAACAGACTATAAAGGGAGCATTGTAAAATGCAAGATGCATGAAGAAGTGCATCGCTTGGTCCAATATGTCACAAGGTATGACTGCTTTATCACGGGTATTGATTGCGTTGAAGAGTCTGGCAGTGGAGGCACCTGTGACTCTTCTTTTGTCCTAGCACCTGGGGCTGACCTCGGAGAGGCAAACATTGAAGTAGCCCGTCATTCTACTTTTGTCCTAGCACCTGGGGCTGCATTTCCTAACTCAGCATGCAACGGAAAAGGACTGCGTACACTCTTCACATTGAGTTCACTGAACATTGCGATAAACCCTGATATACTCTTGCATCTAACATGTCTGAGAACACTAAATCTGAGTCGCTGCTGGTTCAAAGAACTACCGGAGGAGGTTGGAAAGTTTATACATTTGAGATATCTCAATTTATCTAGCAACGAAGAGCTGGAAAGGTTGCCTGAGACGCTGTGTGATTTATCTAACTTGCAAACTTTGTTGATCAACGATTGTATGCGAATTAGAAAACTACCAGAGAGGATGGGGAAGCTATTGAACTTAAGACACCTTCACATTGATTGTTCTTTTAATCTAGAGAGTTTGCCCAAGGGAATTGCAAAACTGACTTCTCTACAGACACTAGATATGCTCGTCCTACCACAGTATGACAACGAAGAAGCATTTAAGCTGGGAGATTTGAAAATTTTGAACAAGCTTGAAGGGTATCTTCATATATGTAGGTGTGGGAACCTAGAAGATGTGAATGAGGCTGAGAAAGCGGAACTCGTAAACAGAGGAAACCTTGTTGATTTGACTCTAGATTTTGATGGGAGTGAAGAGAGGGGGCTCGAAGATAAAATTATACTTGAAGCTTTACAACCACATCCGAATTTGGAATCTTTAGAGATTCGGAAGTATAGGGGCAGAACCGTGTCTCCAGATTGGATGATGAGTTTAACGAATTTGAGAAGGCTTATCCTGCATTCCTGCCTGGATTGTGAGTCTCTACCTCCTTTGGGGAAACTCCTGTCCCTTGAATCACTCGAAATATGTCATATGAAAAGCATGAAAAAGGTGGGGTGCGAGTTTTTGGGGATAGAAACCGGTGGTGCAGAAAAGTGCTGTTTAGTTTCATTCCCGAAACTGAGGGAACTTCGTTTTTCTTCCTTGTTCAAGTGGAATGCGTGGGTAGGGGCATCAAAGTTCAAGAAAGAAGATAGTTCGACCCAAATAATGCCATGCCTCATGTCCTTAGAGATTGACTCCTGCTTTTGCGTAAAGGCTTTGCCACACTTCTTGAAAGAGATACCGCTGCAGAATTTGACGATCAGCAAGTGTAAGATCCTCCAAAAACATTGCCAAAAGTCGAAAGGAAAAGAGTGGTCCAAGATTTCTCACATCCCAAATATCAAATTTAACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

966

Amino Acids

109.44

Weight (kDa)

6.45

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 18 - 77 1.4e-13 Rx N-terminal domain
NB-ARC PF00931 195 - 354 5.3e-25 NB-ARC domain
WHD_DRP PF23559 442 - 515 1.5e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 601 - 934 2e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 721 - 844 6e-32 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021196)

Species Orthologous Gene IDs
malus_domestica MD17G1224300.v1.1
prunus_persica Prupe.3G100000_v2.0.a1
pyrus_communis pycom17g22800
rosa_laevigata RLG00000019277 RLG00000019281

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
Acc36I ACCTGC 1 cut(s) 1269
Acc65I GGTACC 1 cut(s) 565
AccB1I GGYRCC 2 cut(s) 565, 1613
AccBSI CCGCTC 1 cut(s) 574
AccI GTMKAC 1 cut(s) 1404
AccII CGCG 1 cut(s) 87
AciI CCGC 7 cut(s) 85, 516, 574, 1160, 1462, 2252, 2783
AclI AACGTT 2 cut(s) 779, 2892
AclWI GGATC 2 cut(s) 879, 2809
AcsI RAATTY 7 cut(s) 747, 1450, 2175, 2362, 2434, 2791, 2885
AcyI GRCGYC 2 cut(s) 111, 171
AdeI CACNNNGTG 1 cut(s) 321
AfaI GTAC 2 cut(s) 567, 1753
AfeI AGCGCT 1 cut(s) 214
AfiI CCNNNNNNNGG 4 cut(s) 120, 247, 1660, 2489
AflII CTTAAG 1 cut(s) 2032
AflIII ACRYGT 1 cut(s) 1814
AgeI ACCGGT 1 cut(s) 2576
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 3 cut(s) 1642, 1705, 2463
AloI GAACNNNNNNTCC 2 cut(s) 2392, 2424
Alw21I GWGCWC 2 cut(s) 72, 198
AlwI GGATC 2 cut(s) 879, 2809
Aor51HI AGCGCT 1 cut(s) 214
AoxI GGCC 1 cut(s) 1432
ApeKI GCWGC 8 cut(s) 177, 207, 210, 215, 932, 1712, 1842, 2785
ApoI RAATTY 7 cut(s) 747, 1450, 2175, 2362, 2434, 2791, 2885
ArsI GACNNNNNNTTYG 2 cut(s) 1613, 1645
AsiGI ACCGGT 1 cut(s) 2576
Asp700I GAANNNNTTC 1 cut(s) 99
Asp718I GGTACC 1 cut(s) 565
AspLEI GCGC 2 cut(s) 215, 1016
AspS9I GGNCC 2 cut(s) 1543, 2856
AsuC2I CCSGG 2 cut(s) 463, 649
AsuHPI GGTGA 1 cut(s) 307
AsuII TTCGAA 1 cut(s) 355
AvaII GGWCC 2 cut(s) 1543, 2856
BanI GGYRCC 2 cut(s) 565, 1613
BanII GRGCYC 2 cut(s) 72, 2322
BauI CACGAG 1 cut(s) 319
Bbv12I GWGCWC 2 cut(s) 72, 198
BbvI GCAGC 8 cut(s) 164, 194, 202, 222, 944, 1699, 1829, 2772
BccI CCATC 4 cut(s) 875, 979, 2008, 2294
BceAI ACGGC 1 cut(s) 203
BcgI CGANNNNNNTGC 2 cut(s) 1075, 1109
BciT130I CCWGG 3 cut(s) 1644, 1707, 2465
BclI TGATCA 3 cut(s) 664, 1362, 1974
BcnI CCSGG 2 cut(s) 463, 649
BfmI CTRYAG 3 cut(s) 208, 2105, 2786
BfoI RGCGCY 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 2032
BfuAI ACCTGC 1 cut(s) 1269
BglII AGATCT 1 cut(s) 962
BisI GCNGC 8 cut(s) 178, 208, 211, 216, 933, 1713, 1843, 2786
BlsI GCNGC 8 cut(s) 179, 209, 212, 217, 934, 1714, 1844, 2787
Bme1390I CCNGG 5 cut(s) 463, 649, 1644, 1707, 2465
Bme18I GGWCC 2 cut(s) 1543, 2856
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 1543, 2856
BmiI GGNNCC 5 cut(s) 62, 138, 567, 1615, 2222
BmrFI CCNGG 5 cut(s) 463, 649, 1644, 1707, 2465
BmsI GCATC 8 cut(s) 833, 1021, 1063, 1449, 1509, 1543, 1816, 2678
BplI GAGNNNNNCTC 2 cut(s) 2720, 2752
BpmI CTGGAG 2 cut(s) 10, 2394
Bpu10I CCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 355
BpuMI CCSGG 2 cut(s) 463, 649
Bsa29I ATCGAT 1 cut(s) 827
BsaHI GRCGYC 2 cut(s) 111, 171
BsaI GGTCTC 2 cut(s) 28, 1169
BsaJI CCNNGG 7 cut(s) 18, 731, 1155, 1643, 1654, 1706, 2079
BsaWI WCCGGW 2 cut(s) 1861, 2576
BsaXI ACNNNNNCTCC 2 cut(s) 2392, 2422
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 247, 1660, 2489
Bse118I RCCGGY 1 cut(s) 2576
Bse1I ACTGG 1 cut(s) 140
Bse3DI GCAATG 3 cut(s) 972, 1779, 2827
BseBI CCWGG 3 cut(s) 1644, 1707, 2465
BseCI ATCGAT 1 cut(s) 827
BseDI CCNNGG 7 cut(s) 18, 731, 1155, 1643, 1654, 1706, 2079
BseGI GGATG 7 cut(s) 886, 1078, 1380, 2019, 2356, 2424, 2871
BseLI CCNNNNNNNGG 4 cut(s) 120, 247, 1660, 2489
BseMI GCAATG 3 cut(s) 972, 1779, 2827
BseNI ACTGG 1 cut(s) 140
BseRI GAGGAG 2 cut(s) 149, 1880
BseXI GCAGC 8 cut(s) 164, 194, 202, 222, 944, 1699, 1829, 2772
BseYI CCCAGC 1 cut(s) 2161
BsgI GTGCAG 2 cut(s) 951, 2604
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 1 cut(s) 1434
BshNI GGYRCC 2 cut(s) 565, 1613
BshTI ACCGGT 1 cut(s) 2576
BshVI ATCGAT 1 cut(s) 827
BsiHKAI GWGCWC 2 cut(s) 72, 198
BsiSI CCGG 4 cut(s) 462, 649, 1862, 2577
BslFI GGGAC 3 cut(s) 206, 1245, 2488
BslI CCNNNNNNNGG 4 cut(s) 120, 247, 1660, 2489
BsmBI CGTCTC 2 cut(s) 525, 1931
BsmFI GGGAC 3 cut(s) 206, 1245, 2488
BsmI GAATGC 3 cut(s) 1445, 2455, 2659
BsnI GGCC 1 cut(s) 1434
Bso31I GGTCTC 2 cut(s) 28, 1169
Bsp119I TTCGAA 1 cut(s) 355
Bsp1286I GDGCHC 3 cut(s) 72, 198, 2322
BspACI CCGC 7 cut(s) 85, 516, 574, 1160, 1462, 2252, 2783
BspANI GGCC 1 cut(s) 1434
BspDI ATCGAT 1 cut(s) 827
BspFNI CGCG 1 cut(s) 87
BspHI TCATGA 1 cut(s) 1279
BspLI GGNNCC 5 cut(s) 62, 138, 567, 1615, 2222
BspMAI CTGCAG 2 cut(s) 212, 2790
BspMI ACCTGC 1 cut(s) 1269
BspPI GGATC 2 cut(s) 879, 2809
BspQI GCTCTTC 1 cut(s) 1911
BspT104I TTCGAA 1 cut(s) 355
BspT107I GGYRCC 2 cut(s) 565, 1613
BspTI CTTAAG 1 cut(s) 2032
BspTNI GGTCTC 2 cut(s) 28, 1169
BsrBI CCGCTC 1 cut(s) 574
BsrDI GCAATG 3 cut(s) 972, 1779, 2827
BsrFI RCCGGY 1 cut(s) 2576
BsrI ACTGG 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 2576
BssECI CCNNGG 7 cut(s) 18, 731, 1155, 1643, 1654, 1706, 2079
BssNI GRCGYC 2 cut(s) 111, 171
BssSI CACGAG 1 cut(s) 319
BssT1I CCWWGG 3 cut(s) 18, 1155, 2079
Bst2BI CACGAG 1 cut(s) 319
Bst2UI CCWGG 3 cut(s) 1644, 1707, 2465
Bst4CI ACNGT 3 cut(s) 1194, 2136, 2404
Bst6I CTCTTC 6 cut(s) 113, 1590, 1630, 1763, 1911, 2304
BstACI GRCGYC 2 cut(s) 111, 171
BstAFI CTTAAG 1 cut(s) 2032
BstBI TTCGAA 1 cut(s) 355
BstC8I GCNNGC 4 cut(s) 72, 175, 1136, 1732
BstENI CCTNNNNNAGG 1 cut(s) 1658
BstF5I GGATG 7 cut(s) 886, 1078, 1380, 2019, 2356, 2424, 2871
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 2 cut(s) 215, 1016
BstNI CCWGG 3 cut(s) 1644, 1707, 2465
BstNSI RCATGY 3 cut(s) 1238, 1734, 1818
BstSCI CCNGG 5 cut(s) 461, 647, 1642, 1705, 2463
BstSFI CTRYAG 3 cut(s) 208, 2105, 2786
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 8 cut(s) 164, 194, 202, 222, 944, 1699, 1829, 2772
BstX2I RGATCY 3 cut(s) 871, 962, 2814
BstYI RGATCY 3 cut(s) 871, 962, 2814
Bsu15I ATCGAT 1 cut(s) 827
BsuRI GGCC 1 cut(s) 1434
BsuTUI ATCGAT 1 cut(s) 827
BtgZI GCGATG 2 cut(s) 1472, 1520
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 7 cut(s) 886, 1078, 1380, 2019, 2356, 2424, 2871
BtsI GCAGTG 2 cut(s) 1023, 1612
BtsIMutI CAGTG 5 cut(s) 333, 1023, 1199, 1612, 1772
BveI ACCTGC 1 cut(s) 1269
Cac8I GCNNGC 4 cut(s) 72, 175, 1136, 1732
CciI TCATGA 1 cut(s) 1279
CfoI GCGC 2 cut(s) 215, 1016
Cfr10I RCCGGY 1 cut(s) 2576
Cfr13I GGNCC 2 cut(s) 1543, 2856
ClaI ATCGAT 1 cut(s) 827
CseI GACGC 3 cut(s) 119, 179, 1948
Csp6I GTAC 2 cut(s) 566, 1752
CspAI ACCGGT 1 cut(s) 2576
CspCI CAANNNNNGTGG 2 cut(s) 2751, 2786
CviQI GTAC 2 cut(s) 566, 1752
DraIII CACNNNGTG 1 cut(s) 321
Eam1104I CTCTTC 6 cut(s) 113, 1590, 1630, 1763, 1911, 2304
EarI CTCTTC 6 cut(s) 113, 1590, 1630, 1763, 1911, 2304
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 3 cut(s) 18, 1155, 2079
Eco24I GRGCYC 2 cut(s) 72, 2322
Eco31I GGTCTC 2 cut(s) 28, 1169
Eco32I GATATC 1 cut(s) 1895
Eco47I GGWCC 2 cut(s) 1543, 2856
Eco47III AGCGCT 1 cut(s) 214
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 1658
EcoRII CCWGG 3 cut(s) 1642, 1705, 2463
EcoRV GATATC 1 cut(s) 1895
EcoT14I CCWWGG 3 cut(s) 18, 1155, 2079
EcoT22I ATGCAT 1 cut(s) 1524
EcoT38I GRGCYC 2 cut(s) 72, 2322
ErhI CCWWGG 3 cut(s) 18, 1155, 2079
Esp3I CGTCTC 2 cut(s) 525, 1931
FalI AAGNNNNNCTT 6 cut(s) 287, 319, 1374, 1406, 2184, 2216
FaqI GGGAC 3 cut(s) 206, 1245, 2488
FauI CCCGC 1 cut(s) 1153
FauNDI CATATG 1 cut(s) 2529
FbaI TGATCA 3 cut(s) 664, 1362, 1974
FblI GTMKAC 1 cut(s) 1404
Fnu4HI GCNGC 8 cut(s) 178, 208, 211, 216, 933, 1713, 1843, 2786
FokI GGATG 7 cut(s) 893, 1085, 1387, 2026, 2343, 2431, 2858
FriOI GRGCYC 2 cut(s) 72, 2322
Fsp4HI GCNGC 8 cut(s) 178, 208, 211, 216, 933, 1713, 1843, 2786
GlaI GCGC 2 cut(s) 214, 1015
GluI GCNGC 8 cut(s) 178, 208, 211, 216, 933, 1713, 1843, 2786
GsaI CCCAGC 1 cut(s) 2165
GsuI CTGGAG 2 cut(s) 10, 2394
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 1 cut(s) 1434
HapII CCGG 4 cut(s) 462, 649, 1862, 2577
HgaI GACGC 3 cut(s) 119, 179, 1948
HhaI GCGC 2 cut(s) 215, 1016
Hin1I GRCGYC 2 cut(s) 111, 171
Hin6I GCGC 2 cut(s) 213, 1014
HinP1I GCGC 2 cut(s) 213, 1014
HincII GTYRAC 1 cut(s) 1405
HindII GTYRAC 1 cut(s) 1405
HindIII AAGCTT 5 cut(s) 279, 801, 914, 2186, 2343
HpaII CCGG 4 cut(s) 462, 649, 1862, 2577
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 4 cut(s) 1405, 1754, 1772, 2263
Hpy8I GTNNAC 4 cut(s) 1405, 1754, 1772, 2263
HpyCH4III ACNGT 3 cut(s) 1194, 2136, 2404
HpyCH4IV ACGT 3 cut(s) 192, 779, 2892
HpySE526I ACGT 3 cut(s) 192, 779, 2892
Hsp92I GRCGYC 2 cut(s) 111, 171
HspAI GCGC 2 cut(s) 213, 1014
KpnI GGTACC 1 cut(s) 569
Ksp22I TGATCA 3 cut(s) 664, 1362, 1974
LguI GCTCTTC 1 cut(s) 1911
LmnI GCTCC 4 cut(s) 48, 136, 989, 1500
Lsp1109I GCAGC 8 cut(s) 164, 194, 202, 222, 944, 1699, 1829, 2772
LweI GCATC 8 cut(s) 833, 1021, 1063, 1449, 1509, 1543, 1816, 2678
MaeII ACGT 3 cut(s) 192, 779, 2892
MaeIII GTNAC 2 cut(s) 1552, 1619
MbiI CCGCTC 1 cut(s) 574
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 3 cut(s) 871, 962, 2814
MhlI GDGCHC 3 cut(s) 72, 198, 2322
MlyI GAGTC 9 cut(s) 24, 521, 704, 1607, 1616, 1846, 2281, 2483, 2729
MmeI TCCRAC 2 cut(s) 135, 1852
Mph1103I ATGCAT 1 cut(s) 1524
MroXI GAANNNNTTC 1 cut(s) 99
MseI TTAA 9 cut(s) 429, 497, 1040, 1176, 2033, 2061, 2157, 2429, 2889
MspA1I CMGCKG 2 cut(s) 177, 2785
MspCI CTTAAG 1 cut(s) 2032
MspI CCGG 4 cut(s) 462, 649, 1862, 2577
MspR9I CCNGG 5 cut(s) 463, 649, 1644, 1707, 2465
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 3 cut(s) 1445, 2455, 2659
MvaI CCWGG 3 cut(s) 1644, 1707, 2465
MvnI CGCG 1 cut(s) 87
NciI CCSGG 2 cut(s) 463, 649
NdeI CATATG 1 cut(s) 2529
NlaIV GGNNCC 5 cut(s) 62, 138, 567, 1615, 2222
NmeAIII GCCGAG 2 cut(s) 139, 756
NmuCI GTSAC 2 cut(s) 1552, 1619
NsiI ATGCAT 1 cut(s) 1524
NspI RCATGY 3 cut(s) 1238, 1734, 1818
NspV TTCGAA 1 cut(s) 355
PaeI GCATGC 1 cut(s) 1734
PagI TCATGA 1 cut(s) 1279
PciI ACATGT 1 cut(s) 1814
PciSI GCTCTTC 1 cut(s) 1911
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 3 cut(s) 1445, 2455, 2659
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 6 cut(s) 78, 227, 1199, 2369, 2380, 2510
PfoI TCCNGGA 1 cut(s) 461
PinAI ACCGGT 1 cut(s) 2576
PkrI GCNGC 8 cut(s) 179, 209, 212, 217, 934, 1714, 1844, 2787
PleI GAGTC 9 cut(s) 24, 520, 704, 1606, 1616, 1845, 2281, 2482, 2729
PpsI GAGTC 9 cut(s) 24, 520, 704, 1606, 1616, 1845, 2281, 2482, 2729
PscI ACATGT 1 cut(s) 1814
PsiI TTATAA 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 72
Psp1406I AACGTT 2 cut(s) 779, 2892
Psp6I CCWGG 3 cut(s) 1642, 1705, 2463
PspFI CCCAGC 1 cut(s) 2161
PspGI CCWGG 3 cut(s) 1642, 1705, 2463
PspN4I GGNNCC 5 cut(s) 62, 138, 567, 1615, 2222
PspPI GGNCC 2 cut(s) 1543, 2856
PstI CTGCAG 2 cut(s) 212, 2790
PsuI RGATCY 3 cut(s) 871, 962, 2814
PvuII CAGCTG 1 cut(s) 177
RsaI GTAC 2 cut(s) 567, 1753
RsaNI GTAC 2 cut(s) 566, 1752
SacI GAGCTC 1 cut(s) 72
SalI GTCGAC 1 cut(s) 1403
SapI GCTCTTC 1 cut(s) 1911
SaqAI TTAA 9 cut(s) 429, 497, 1040, 1176, 2033, 2061, 2157, 2429, 2889
SatI GCNGC 8 cut(s) 178, 208, 211, 216, 933, 1713, 1843, 2786
Sau96I GGNCC 2 cut(s) 1543, 2856
SchI GAGTC 9 cut(s) 24, 521, 704, 1607, 1616, 1846, 2281, 2483, 2729
ScrFI CCNGG 5 cut(s) 463, 649, 1644, 1707, 2465
SduI GDGCHC 3 cut(s) 72, 198, 2322
SfaNI GCATC 8 cut(s) 833, 1021, 1063, 1449, 1509, 1543, 1816, 2678
SfcI CTRYAG 3 cut(s) 208, 2105, 2786
SfuI TTCGAA 1 cut(s) 355
SinI GGWCC 2 cut(s) 1543, 2856
SmlI CTYRAG 1 cut(s) 2032
SmoI CTYRAG 1 cut(s) 2032
SphI GCATGC 1 cut(s) 1734
SsiI CCGC 7 cut(s) 85, 516, 574, 1160, 1462, 2252, 2783
SspI AATATT 1 cut(s) 1326
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 5 cut(s) 461, 647, 1642, 1705, 2463
StyI CCWWGG 3 cut(s) 18, 1155, 2079
TaaI ACNGT 3 cut(s) 1194, 2136, 2404
TaiI ACGT 3 cut(s) 195, 782, 2895
TfiI GAWTC 6 cut(s) 78, 227, 1199, 2369, 2380, 2510
Tru1I TTAA 9 cut(s) 429, 497, 1040, 1176, 2033, 2061, 2157, 2429, 2889
Tru9I TTAA 9 cut(s) 429, 497, 1040, 1176, 2033, 2061, 2157, 2429, 2889
TscAI CASTG 5 cut(s) 340, 1023, 1199, 1612, 1779
TseFI GTSAC 2 cut(s) 1552, 1619
TseI GCWGC 8 cut(s) 177, 207, 210, 215, 932, 1712, 1842, 2785
Tsp45I GTSAC 2 cut(s) 1552, 1619
TspDTI ATGAA 6 cut(s) 1463, 1539, 2192, 2546, 2555, 2595
TspGWI ACGGA 1 cut(s) 1752
TspRI CASTG 5 cut(s) 340, 1023, 1199, 1612, 1779
Vha464I CTTAAG 1 cut(s) 2032
VpaK11BI GGWCC 2 cut(s) 1543, 2856
XagI CCTNNNNNAGG 1 cut(s) 1658
XapI RAATTY 7 cut(s) 747, 1450, 2175, 2362, 2434, 2791, 2885
XbaI TCTAGA 3 cut(s) 103, 2065, 2290
XceI RCATGY 3 cut(s) 1238, 1734, 1818
XcmI CCANNNNNNNNNTGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 1404
XmnI GAANNNNTTC 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.