RLG00000019277

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021196
Physical Location & Seq
Forward (+)
49530721 .. 49533471
2751 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019277

Sequence Viewer

Length: 2751 bp
ATGGCTGGAGCACTTGTTCGTCGACTGATACCCTATTCAGTCAGAACAAATGAAATAGAGAAGTTCACCTCTGATCTCGGAGGAGTAAAAGCCGTGCTTCAGGTTGCCGATCAGAGTGAAGAAAAGGAGATCCATATCCGCTGGTTGAATGAGCTCAGAGATTTAAGGTGCGACATGGAGGACGCGTTGGATGAATGGAACACTGCCCTTCTCAAATCAAAAATTGGGAAAGAAGAAGATGGGTCCTCAAACAAGGTACTCTTCGGCGGCTCTTGCTTTAGTTTCAGTCATGTGGATCGAGTGGGTCAACGCCGTCATATTGCTGTCAAGATGAAAGATTTGACTAAAAGACTCGACTCAATTGCCGAGTTGTGGCGTCAATATAGGCCTACAGAAGTCACAAGCGTTGAGGTTAAAGAGAGAGAGACCAACTCTTTTCTTGATATAAGCTCTGTATACGGTCGAGAAGGAGAACGGGAAAATCTAATAGAAGCGTTGCTATCTGAGAGCAGGGTGGTGCCCGATGTCGTCGCTATTGTGGGGATGGGTGGATTGGGGAAAACCACTCTTGCCCAATTAGCATATAATGACGGCAAGGTCTGTGCCCATTTTTATCCGCAAATGTGGGTGTGCGTCTCAGATCCTTTCGATGTGAACAGAGTTGCCATTGCAATTCTTGAAGCTATATCCGGCGAAAGGGTAGACTACTCACTTGGAAGGAATTTTTTGTTTTCCCGTATAAGGGACTGTATTGCTGGAAAGAAGTTCTTACTTGTCCTAGACGATGTATGGAATCAAGACCCCTGGAAGTGGGAACCTTTGAAGCGGCCGTTTTGTTATGGTGCTGTTGGAAGTAAAATTTTGGTGACAACAAGAAATGAGAATGTTGCTAGAATGATGGCAACAAGTCCTGTAATTACTTTGGGGGCATTGTCTGAGGAAGATAGCTGGTCGTTGTTCAGCAATTCAGCAGGTCTGAGTAGGGATGCGAGCTATCGCACACAATTTCAAGAAATTGGTAGAGAAATTGTAAGCAAGTGCAAGGGCATGCCGCTTGCTTTGAAGACCTTGGGAGCTCTCTTGCGGTCCAAAAACACCAGAGAAGAATGGGAGAGTGTTTTGAATAGTGAAACGTGGAGATTGGAAGATGTGGGTGGGAACATTTTTGCCGCACTCCGATTGAGTTATTACGACTTGGTTCCTTCTGAGAGACGTTGTTTCTCATACTGTGCTATTTTTCCAAAAGATTACATGATTGAGACTGACGGTTTGATCAAGATGTGGATGTCACAAGGTTTTCTTGATTCCGGCAGCAAAATAGAGAAGGAATTGATAGGTCAGAGATACTTTATGGAGTTAGCATCGAGATCTTTCTTCCAGGAATTTGAAAAAGATTATAGAGGAAATATCATAAGATGCAAGATGCATGACTTGGTGCATGATTTGGCACAGTCTCTCACAGAACATGAATGCATTCTCATAGAGGTTTCTGGTGCTACACGGGAAATGAAGCGACATCTTGATAAACCTCGCCATGCTACCTTTGTGCTTGCAGCTGGTGCTACTTTTCCTATCTCAACATGCAATGAGCAAACTCTGCGTACTGCCTTTTTTACATGCTCCACAAATACTATCATAACTCATGATGTATTTGTACATCTAAAATGTCTTAGGACATTAAATTTGAGTGATTTCTGGTTCAAAGAACTACCCAAGGAGATTGGTAAGTTTATGCATTTGAGATATCTCAATTTATCTGGTAATGACGAGTTGAAGACATTGCCTGAGACACTATGTGATTTGGCTAACTTGCAAACCTTGTTGGTGAGTAGGTGCATGCAACTGGAAAAACTTCCAGACCGCATGGAGAAACTTGTTAGCTTAAGACATCTTCATGTTGATGGATCTTTTAATCTATTGACGTTACCAAAAGGAATTGCGAAGCTAGCTTCTCTTCGGACCCTAGACATGCTTGTTGTACCACCTTCTGACAATCTGCAAGCATTTCAGCTTGAAGATATGAAAAAGTTGAACAACGTTGAAGGGTATCTTCGTATCTGCAGGTGCGGAAATCTGAGAAATGTGCAAGAGGCTGAAAATGCACAACTGTTTTGTAGAAGCCAGATTCTTGATTTGAATCTAGATTTCAGTTGGAGCGAAGGCCGGAGGCTCAAAGATGAAATAGTACTTGAAGCCTTACGTCCGCAGCCCAATTTGGAATCTTTGGAAATTAGGAAGTACAGAGGCACCACCATCTCCCCACATTGGTTGATGTGGTTAGACAATTTGAGAAGGCTTGTCCTATATTCCTGCCAATACTGTGAGTCTTTACCCCCTCTGGGAAAACTTGCTTCACTCGAATCACTTAAAATATGTCTTATGAAAGGTGTGAAAAAGGTGGGGCTTGAGTTTTTGGGAATTGAAACTGATGGTGGAGAAAAATCATCATTTGTTTCATTCCCAAAATTGAGAGAACTTTGTTTTACAGAATTGAGCAACTGGAAAGTGTGGGAAGGGAGTGCAGGGTTTACAGAAGAACATGATTCTATCGCAATAATGCCGCTTCTTAATTCCTTAGAGATTGACTCCTGCTTCTTGTTACAAGTGCTACCAGATTTCCTGAAGATGACAACATTGCAGAATTTGACCGTCACTGAGTGTGAAATTCTTCAAGAACATTGCAAGGAGGGAACAGGAAAGGAGTGGGTCAAAATTTCTCAGATCCCGAATGTCAAATTTGTTAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

917

Amino Acids

104.57

Weight (kDa)

6.12

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 17 - 81 3.7e-09 Rx N-terminal domain
NB-ARC PF00931 158 - 325 2.2e-33 NB-ARC domain
WHD_DRP PF23559 412 - 483 5.9e-26 Disease resistance protein Winged helix domain
LRR_14 PF23598 538 - 889 1.9e-24 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 671 - 792 3.7e-29 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021196)

Species Orthologous Gene IDs
malus_domestica MD17G1224300.v1.1
prunus_persica Prupe.3G100000_v2.0.a1
pyrus_communis pycom17g22800
rosa_laevigata RLG00000019277 RLG00000019281

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2052
AasI GACNNNNNNGTC 1 cut(s) 596
Acc36I ACCTGC 2 cut(s) 962, 2052
AccB1I GGYRCC 2 cut(s) 517, 2246
AccI GTMKAC 3 cut(s) 22, 456, 702
AccII CGCG 1 cut(s) 185
AclI AACGTT 1 cut(s) 2037
AclWI GGATC 5 cut(s) 124, 303, 635, 1912, 2716
AcoI YGGCCR 1 cut(s) 827
AcsI RAATTY 8 cut(s) 721, 858, 1382, 1681, 2641, 2664, 2712, 2735
AcuI CTGAAG 2 cut(s) 83, 2642
AcyI GRCGYC 1 cut(s) 376
AdeI CACNNNGTG 2 cut(s) 1796, 2658
AfaI GTAC 6 cut(s) 258, 1603, 1656, 1980, 2187, 2240
AfiI CCNNNNNNNGG 7 cut(s) 372, 696, 741, 742, 810, 2265, 2339
AflII CTTAAG 1 cut(s) 1882
AflIII ACRYGT 1 cut(s) 183
AjnI CCWGG 2 cut(s) 803, 1377
AloI GAACNNNNNNTCC 1 cut(s) 32
Alw21I GWGCWC 3 cut(s) 13, 156, 1078
Alw26I GTCTC 6 cut(s) 419, 640, 1204, 1253, 1458, 1781
AlwI GGATC 5 cut(s) 124, 303, 635, 1912, 2716
AoxI GGCC 3 cut(s) 386, 827, 2161
ApeKI GCWGC 3 cut(s) 1311, 1553, 2206
ApoI RAATTY 8 cut(s) 721, 858, 1382, 1681, 2641, 2664, 2712, 2735
ArsI GACNNNNNNTTYG 4 cut(s) 1252, 1284, 1666, 1698
Asp700I GAANNNNTTC 3 cut(s) 1473, 1851, 2667
AspS9I GGNCC 3 cut(s) 243, 1086, 1959
AsuHPI GGTGA 3 cut(s) 58, 877, 1837
AsuNHI GCTAGC 1 cut(s) 1945
AvaII GGWCC 3 cut(s) 243, 1086, 1959
BaeGI GKGCMC 2 cut(s) 522, 607
BanI GGYRCC 2 cut(s) 517, 2246
BanII GRGCYC 2 cut(s) 156, 1078
BbsI GAAGAC 2 cut(s) 1070, 1781
Bbv12I GWGCWC 3 cut(s) 13, 156, 1078
BbvI GCAGC 3 cut(s) 1323, 1565, 2218
BccI CCATC 6 cut(s) 233, 538, 892, 1895, 2261, 2423
BceAI ACGGC 4 cut(s) 77, 297, 607, 814
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 2 cut(s) 805, 1379
BclI TGATCA 1 cut(s) 1272
BcoDI GTCTC 6 cut(s) 419, 640, 1204, 1253, 1458, 1781
BfaI CTAG 5 cut(s) 779, 891, 1946, 1964, 2141
BfmI CTRYAG 2 cut(s) 390, 2059
BfrI CTTAAG 1 cut(s) 1882
BfuAI ACCTGC 2 cut(s) 962, 2052
BglII AGATCT 1 cut(s) 1367
BisI GCNGC 8 cut(s) 268, 827, 1052, 1170, 1312, 1554, 2207, 2561
BlsI GCNGC 8 cut(s) 269, 828, 1053, 1171, 1313, 1555, 2208, 2562
BmcAI AGTACT 1 cut(s) 2187
Bme1390I CCNGG 2 cut(s) 805, 1379
Bme18I GGWCC 3 cut(s) 243, 1086, 1959
BmgT120I GGNCC 3 cut(s) 243, 1086, 1959
BmiI GGNNCC 6 cut(s) 244, 519, 816, 1200, 1961, 2248
BmrFI CCNGG 2 cut(s) 805, 1379
BmsI GCATC 4 cut(s) 976, 1370, 1406, 1413
BmtI GCTAGC 1 cut(s) 1949
BpiI GAAGAC 2 cut(s) 1070, 1781
BplI GAGNNNNNCTC 4 cut(s) 416, 448, 2570, 2602
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 2426
BsaBI GATNNNNATC 2 cut(s) 134, 2136
BsaHI GRCGYC 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 803, 1068, 1713
BsaXI ACNNNNNCTCC 3 cut(s) 30, 170, 200
Bsc4I CCNNNNNNNGG 7 cut(s) 372, 696, 741, 742, 810, 2265, 2339
Bse1I ACTGG 2 cut(s) 1848, 2504
Bse3DI GCAATG 5 cut(s) 666, 1591, 1778, 2633, 2677
Bse8I GATNNNNATC 2 cut(s) 134, 2136
BseBI CCWGG 2 cut(s) 805, 1379
BseDI CCNNGG 3 cut(s) 803, 1068, 1713
BseGI GGATG 4 cut(s) 196, 549, 991, 1290
BseJI GATNNNNATC 2 cut(s) 134, 2136
BseLI CCNNNNNNNGG 7 cut(s) 372, 696, 741, 742, 810, 2265, 2339
BseMI GCAATG 5 cut(s) 666, 1591, 1778, 2633, 2677
BseNI ACTGG 2 cut(s) 1848, 2504
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 2 cut(s) 522, 607
BseX3I CGGCCG 1 cut(s) 827
BseXI GCAGC 3 cut(s) 1323, 1565, 2218
BsgI GTGCAG 1 cut(s) 2541
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 2 cut(s) 463, 830
BshFI GGCC 3 cut(s) 388, 829, 2163
BshNI GGYRCC 2 cut(s) 517, 2246
BsiEI CGRYCG 2 cut(s) 463, 830
BsiHKAI GWGCWC 3 cut(s) 13, 156, 1078
BsiSI CCGG 3 cut(s) 690, 1308, 2164
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 7 cut(s) 372, 696, 741, 742, 810, 2265, 2339
BsmAI GTCTC 6 cut(s) 419, 640, 1204, 1253, 1458, 1781
BsmBI CGTCTC 2 cut(s) 640, 1204
BsmFI GGGAC 1 cut(s) 758
BsmI GAATGC 2 cut(s) 1473, 1475
BsnI GGCC 3 cut(s) 388, 829, 2163
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 5 cut(s) 13, 156, 522, 607, 1078
Bsp1407I TGTACA 1 cut(s) 1654
Bsp143I GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
BspANI GGCC 3 cut(s) 388, 829, 2163
BspFNI CGCG 1 cut(s) 185
BspHI TCATGA 1 cut(s) 1642
BspLI GGNNCC 6 cut(s) 244, 519, 816, 1200, 1961, 2248
BspMAI CTGCAG 1 cut(s) 2063
BspMI ACCTGC 2 cut(s) 962, 2052
BspOI GCTAGC 1 cut(s) 1949
BspPI GGATC 5 cut(s) 124, 303, 635, 1912, 2716
BspT107I GGYRCC 2 cut(s) 517, 2246
BspTI CTTAAG 1 cut(s) 1882
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 5 cut(s) 666, 1591, 1778, 2633, 2677
BsrGI TGTACA 1 cut(s) 1654
BsrI ACTGG 2 cut(s) 1848, 2504
BssECI CCNNGG 3 cut(s) 803, 1068, 1713
BssMI GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
BssNAI GTATAC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 376
BssT1I CCWWGG 2 cut(s) 1068, 1713
Bst1107I GTATAC 1 cut(s) 457
Bst2UI CCWGG 2 cut(s) 805, 1379
Bst4CI ACNGT 8 cut(s) 461, 749, 1229, 1268, 1452, 2109, 2321, 2650
Bst6I CTCTTC 2 cut(s) 266, 1959
BstACI GRCGYC 1 cut(s) 376
BstAFI CTTAAG 1 cut(s) 1882
BstAPI GCANNNNNTGC 2 cut(s) 1559, 1597
BstAUI TGTACA 1 cut(s) 1654
BstC8I GCNNGC 7 cut(s) 991, 1049, 1056, 1551, 1838, 1947, 2001
BstF5I GGATG 4 cut(s) 196, 549, 991, 1290
BstFNI CGCG 1 cut(s) 185
BstKTI GATC 9 cut(s) 76, 112, 132, 298, 643, 1275, 1370, 1907, 2724
BstMAI GTCTC 6 cut(s) 419, 640, 1204, 1253, 1458, 1781
BstMBI GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
BstMCI CGRYCG 2 cut(s) 463, 830
BstMWI GCNNNNNNNGC 6 cut(s) 273, 578, 1559, 1597, 1946, 2099
BstNI CCWGG 2 cut(s) 805, 1379
BstNSI RCATGY 5 cut(s) 1051, 1584, 1620, 1840, 1972
BstSCI CCNGG 2 cut(s) 803, 1377
BstSFI CTRYAG 2 cut(s) 390, 2059
BstSLI GKGCMC 2 cut(s) 522, 607
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 3 cut(s) 1323, 1565, 2218
BstV2I GAAGAC 2 cut(s) 1070, 1781
BstX2I RGATCY 5 cut(s) 129, 640, 1367, 1904, 2721
BstYI RGATCY 5 cut(s) 129, 640, 1367, 1904, 2721
BstZ17I GTATAC 1 cut(s) 457
BstZI CGGCCG 1 cut(s) 827
BsuRI GGCC 3 cut(s) 388, 829, 2163
BtsCI GGATG 4 cut(s) 196, 549, 991, 1290
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 2 cut(s) 201, 2652
BveI ACCTGC 2 cut(s) 962, 2052
Cac8I GCNNGC 7 cut(s) 991, 1049, 1056, 1551, 1838, 1947, 2001
CciI TCATGA 1 cut(s) 1642
Cfr13I GGNCC 3 cut(s) 243, 1086, 1959
CseI GACGC 3 cut(s) 191, 365, 622
Csp6I GTAC 6 cut(s) 257, 1602, 1655, 1979, 2186, 2239
CspCI CAANNNNNGTGG 2 cut(s) 2250, 2285
CviQI GTAC 6 cut(s) 257, 1602, 1655, 1979, 2186, 2239
DpnI GATC 9 cut(s) 75, 111, 131, 297, 642, 1274, 1369, 1906, 2723
DpnII GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
DraIII CACNNNGTG 2 cut(s) 1796, 2658
DrdI GACNNNNNNGTC 1 cut(s) 596
DseDI GACNNNNNNGTC 1 cut(s) 596
EaeI YGGCCR 1 cut(s) 827
EagI CGGCCG 1 cut(s) 827
Eam1104I CTCTTC 2 cut(s) 266, 1959
EarI CTCTTC 2 cut(s) 266, 1959
Ecl136II GAGCTC 2 cut(s) 154, 1076
EclXI CGGCCG 1 cut(s) 827
Eco130I CCWWGG 2 cut(s) 1068, 1713
Eco147I AGGCCT 1 cut(s) 388
Eco24I GRGCYC 2 cut(s) 156, 1078
Eco31I GGTCTC 1 cut(s) 419
Eco32I GATATC 1 cut(s) 1745
Eco47I GGWCC 3 cut(s) 243, 1086, 1959
Eco52I CGGCCG 1 cut(s) 827
Eco53kI GAGCTC 2 cut(s) 154, 1076
Eco57I CTGAAG 2 cut(s) 83, 2642
EcoICRI GAGCTC 2 cut(s) 154, 1076
EcoO109I RGGNCCY 1 cut(s) 243
EcoRII CCWGG 2 cut(s) 803, 1377
EcoRV GATATC 1 cut(s) 1745
EcoT14I CCWWGG 2 cut(s) 1068, 1713
EcoT22I ATGCAT 3 cut(s) 1428, 1475, 1737
EcoT38I GRGCYC 2 cut(s) 156, 1078
ErhI CCWWGG 2 cut(s) 1068, 1713
Esp3I CGTCTC 2 cut(s) 640, 1204
FaqI GGGAC 1 cut(s) 758
FbaI TGATCA 1 cut(s) 1272
FblI GTMKAC 3 cut(s) 22, 456, 702
Fnu4HI GCNGC 8 cut(s) 268, 827, 1052, 1170, 1312, 1554, 2207, 2561
FokI GGATG 4 cut(s) 203, 556, 998, 1297
FriOI GRGCYC 2 cut(s) 156, 1078
Fsp4HI GCNGC 8 cut(s) 268, 827, 1052, 1170, 1312, 1554, 2207, 2561
FspBI CTAG 5 cut(s) 779, 891, 1946, 1964, 2141
GluI GCNGC 8 cut(s) 268, 827, 1052, 1170, 1312, 1554, 2207, 2561
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 3 cut(s) 388, 829, 2163
HapII CCGG 3 cut(s) 690, 1308, 2164
HgaI GACGC 3 cut(s) 191, 365, 622
Hin1I GRCGYC 1 cut(s) 376
HincII GTYRAC 2 cut(s) 23, 308
HindII GTYRAC 2 cut(s) 23, 308
HpaII CCGG 3 cut(s) 690, 1308, 2164
HphI GGTGA 3 cut(s) 58, 877, 1837
Hpy166II GTNNAC 7 cut(s) 23, 66, 308, 457, 655, 703, 2529
Hpy8I GTNNAC 7 cut(s) 23, 66, 308, 457, 655, 703, 2529
Hpy99I CGWCG 2 cut(s) 24, 533
HpyCH4III ACNGT 8 cut(s) 461, 749, 1229, 1268, 1452, 2109, 2321, 2650
HpyCH4IV ACGT 5 cut(s) 1133, 1213, 1922, 2037, 2200
HpyF10VI GCNNNNNNNGC 6 cut(s) 273, 578, 1559, 1597, 1946, 2099
HpySE526I ACGT 5 cut(s) 1133, 1213, 1922, 2037, 2200
Hsp92I GRCGYC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 1272
Kzo9I GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
LmnI GCTCC 4 cut(s) 8, 1073, 1625, 2154
Lsp1109I GCAGC 3 cut(s) 1323, 1565, 2218
LweI GCATC 4 cut(s) 976, 1370, 1406, 1413
MaeI CTAG 5 cut(s) 779, 891, 1946, 1964, 2141
MaeII ACGT 5 cut(s) 1133, 1213, 1922, 2037, 2200
MaeIII GTNAC 6 cut(s) 397, 865, 1287, 1923, 2598, 2650
MalI GATC 9 cut(s) 75, 111, 131, 297, 642, 1274, 1369, 1906, 2723
MboI GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 5 cut(s) 129, 640, 1367, 1904, 2721
MhlI GDGCHC 5 cut(s) 13, 156, 522, 607, 1078
MluI ACGCGT 1 cut(s) 183
MlyI GAGTC 4 cut(s) 345, 350, 2333, 2579
MmeI TCCRAC 3 cut(s) 168, 829, 2132
Mph1103I ATGCAT 3 cut(s) 1428, 1475, 1737
MroXI GAANNNNTTC 3 cut(s) 1473, 1851, 2667
MseI TTAA 9 cut(s) 164, 414, 1679, 1883, 1911, 2367, 2568, 2742, 2749
MslI CAYNNNNRTG 2 cut(s) 1893, 1899
MspA1I CMGCKG 2 cut(s) 141, 1556
MspCI CTTAAG 1 cut(s) 1882
MspI CCGG 3 cut(s) 690, 1308, 2164
MspR9I CCNGG 2 cut(s) 805, 1379
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 2 cut(s) 1473, 1475
MvaI CCWGG 2 cut(s) 805, 1379
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 6 cut(s) 273, 578, 1559, 1597, 1946, 2099
NdeII GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
NheI GCTAGC 1 cut(s) 1945
NlaIV GGNNCC 6 cut(s) 244, 519, 816, 1200, 1961, 2248
NmeAIII GCCGAG 1 cut(s) 391
NmuCI GTSAC 4 cut(s) 397, 865, 1287, 2650
NsiI ATGCAT 3 cut(s) 1428, 1475, 1737
NspI RCATGY 5 cut(s) 1051, 1584, 1620, 1840, 1972
PaeI GCATGC 2 cut(s) 1051, 1840
PagI TCATGA 1 cut(s) 1642
PaqCI CACCTGC 1 cut(s) 2052
PceI AGGCCT 1 cut(s) 388
PctI GAATGC 2 cut(s) 1473, 1475
PdmI GAANNNNTTC 3 cut(s) 1473, 1851, 2667
PfeI GAWTC 7 cut(s) 793, 1304, 2125, 2137, 2219, 2360, 2543
PfoI TCCNGGA 1 cut(s) 1377
PkrI GCNGC 8 cut(s) 269, 828, 1053, 1171, 1313, 1555, 2208, 2562
PleI GAGTC 4 cut(s) 345, 350, 2332, 2579
PpsI GAGTC 4 cut(s) 345, 350, 2332, 2579
PpuMI RGGWCCY 1 cut(s) 243
Psp124BI GAGCTC 2 cut(s) 156, 1078
Psp1406I AACGTT 1 cut(s) 2037
Psp5II RGGWCCY 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 803, 1377
PspGI CCWGG 2 cut(s) 803, 1377
PspN4I GGNNCC 6 cut(s) 244, 519, 816, 1200, 1961, 2248
PspPI GGNCC 3 cut(s) 243, 1086, 1959
PspPPI RGGWCCY 1 cut(s) 243
PstI CTGCAG 1 cut(s) 2063
PsuI RGATCY 5 cut(s) 129, 640, 1367, 1904, 2721
PvuII CAGCTG 1 cut(s) 1556
RsaI GTAC 6 cut(s) 258, 1603, 1656, 1980, 2187, 2240
RsaNI GTAC 6 cut(s) 257, 1602, 1655, 1979, 2186, 2239
RseI CAYNNNNRTG 2 cut(s) 1893, 1899
SacI GAGCTC 2 cut(s) 156, 1078
SalI GTCGAC 1 cut(s) 21
SaqAI TTAA 9 cut(s) 164, 414, 1679, 1883, 1911, 2367, 2568, 2742, 2749
SatI GCNGC 8 cut(s) 268, 827, 1052, 1170, 1312, 1554, 2207, 2561
Sau3AI GATC 9 cut(s) 73, 109, 129, 295, 640, 1272, 1367, 1904, 2721
Sau96I GGNCC 3 cut(s) 243, 1086, 1959
ScaI AGTACT 1 cut(s) 2187
SchI GAGTC 4 cut(s) 345, 350, 2333, 2579
ScrFI CCNGG 2 cut(s) 805, 1379
SduI GDGCHC 5 cut(s) 13, 156, 522, 607, 1078
SfaNI GCATC 4 cut(s) 976, 1370, 1406, 1413
SfcI CTRYAG 2 cut(s) 390, 2059
SinI GGWCC 3 cut(s) 243, 1086, 1959
SmiMI CAYNNNNRTG 2 cut(s) 1893, 1899
SmlI CTYRAG 2 cut(s) 1882, 2405
SmoI CTYRAG 2 cut(s) 1882, 2405
SphI GCATGC 2 cut(s) 1051, 1840
SseBI AGGCCT 1 cut(s) 388
SspMI CTAG 5 cut(s) 779, 891, 1946, 1964, 2141
SstI GAGCTC 2 cut(s) 156, 1078
StuI AGGCCT 1 cut(s) 388
StyD4I CCNGG 2 cut(s) 803, 1377
StyI CCWWGG 2 cut(s) 1068, 1713
TaaI ACNGT 8 cut(s) 461, 749, 1229, 1268, 1452, 2109, 2321, 2650
TaiI ACGT 5 cut(s) 1136, 1216, 1925, 2040, 2203
TaqI TCGA 7 cut(s) 22, 298, 354, 463, 648, 1364, 2358
TatI WGTACW 3 cut(s) 1654, 2185, 2238
TauI GCSGC 5 cut(s) 270, 829, 1054, 1172, 2563
TfiI GAWTC 7 cut(s) 793, 1304, 2125, 2137, 2219, 2360, 2543
Tru1I TTAA 9 cut(s) 164, 414, 1679, 1883, 1911, 2367, 2568, 2742, 2749
Tru9I TTAA 9 cut(s) 164, 414, 1679, 1883, 1911, 2367, 2568, 2742, 2749
TscAI CASTG 2 cut(s) 208, 2659
TseFI GTSAC 4 cut(s) 397, 865, 1287, 2650
TseI GCWGC 3 cut(s) 1311, 1553, 2206
Tsp45I GTSAC 4 cut(s) 397, 865, 1287, 2650
TspRI CASTG 2 cut(s) 208, 2659
Vha464I CTTAAG 1 cut(s) 1882
VpaK11BI GGWCC 3 cut(s) 243, 1086, 1959
XapI RAATTY 8 cut(s) 721, 858, 1382, 1681, 2641, 2664, 2712, 2735
XbaI TCTAGA 1 cut(s) 2140
XceI RCATGY 5 cut(s) 1051, 1584, 1620, 1840, 1972
XmiI GTMKAC 3 cut(s) 22, 456, 702
XmnI GAANNNNTTC 3 cut(s) 1473, 1851, 2667
XspI CTAG 5 cut(s) 779, 891, 1946, 1964, 2141
ZrmI AGTACT 1 cut(s) 2187
Zsp2I ATGCAT 3 cut(s) 1428, 1475, 1737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.