RLG00000019281

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0021196
Physical Location & Seq
Forward (+)
49630052 .. 49633535
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019281

Sequence Viewer

Length: 2541 bp
ATGGCTGGAGCACTTGTTCGCCGACTGATGCCCCATTCAGTAGGAACACATGAAATAGAGAAGTTAAGCTCTGACCTTGGAGCAGTAAAAGCCGCGCTTGAGGAGGCCGAGGAGAGACGAGAAGAGGGGCACTGGTTGGATGAGCTCAGAGATTTAAGGTGCGACATGGAGGACGTGTTGGATGAATGGAACACTGCCCTTCTCAAATTAAAAATTGCGAGAGAAGAAGATGAGTCCTCAAACAAGGTACGGTTCAACTCTTGCTTTTGTTCCGGTCATGTGGATCGAGTGGGTCATCGCCGTCATTTTGCTCTCAAGATGAAAGATTTGAGTCAAAGAGTGAATTCACTTGACAATTTCATTACCTCATTTAGATTCAATTCAGTTGAGAATCGTGGAGTTGACTTCTCCGAGAGACTAACAACTACCTCCCTTCTTAATTTTGGCTCTGTATACGGTCGAGAAGGAGAACGGGACAATCTAATAGAAGCGTTGCTATCTGAGACTGAGAGCATGGCGGTGCCCGATGTCGTCGCTATTGTGGGGATGGGTGGATTGGGGAAAACCACTCTTGCCCAATTAGCCTATAATGACCCAACGGTCGTAGTCGATTTTGATCTGCGAACGTGGGTGTGCGTCTCAGAGCCTTTCCTTTGGCAAAGAATTGCCCTTGCAATTCTTGAAGTTATCTCCGGTGAAAGGCAAGACCCGGTTGATTTGGACTCTTTGTCTTCCCGGATAAGGGACTCTATTACTGGAAAGAAGTTCTTACTTGTCCTAGACGATGTATGGAATGAAGACTCCTCAAGATGGGAACTTTTGAAGCAATCCTTGTGTTATGGTGCTGTTGGAAGTAAAATTTTGGTTACAACAAGAAATATGAACGTTGCTAGAATGATGGCAACAACTCCTGTAATTACTTTGGGGGCATTGTCCGAGGAAGATTCCTGGTTCTTGTTCAGCAATATAGCATATAGGAGTATGGATGTGAGCCATCACAAACAAGTTGAAGACATTGGTAGAAAAATTGCAAGCAAGTGCAAGGGCATGCCGCTTGCTTTGAAGACCTTGGGAGATCTCTTGTGGTCCAAACGCACCAGAGAAGAATGGCTGCGTGTTTTGAATAGTGAACTGTGGGAGTTAGAAGAAAAAGTGAGTTCGGACATTTTCGCCCCACTCCGATTGAGTTACTATGACTTGGCTCCTTCTGTAAAACGTTGTTTCTCTTACTGTGCTATTTTTCCAAAAGATTACATAATTGAGACTGACAGTATGATTAAAATGTGGATGTCACAAGGTTTTCTTGATTCTTACAGCGGAATAGAGAAGGAATTGTTAGGTCAGAGATACTTTATGGAATTGGCATCAAGATCTTTCTTCCAGGAATTTGAAAAAGATTATAGAGGAAATATTATAAGATGCAAGATGCATGACTTAGTGCATGATTTGGCCCAGTCTGTTACAGAACATGAATGCTTTCTCATAGAGGTTTCTGGTGCTACACAGGATATGAAGCGAGTCCTTGAAAAACCTCGCCATGGTACCTTTGTGCTTTCAGCTGGTGCTACATTTCCTATCTCAACATGCAATGAGAAAACTCTGCGTACTGCCTTTTTTTTGTGCTCCACAAATGCTGCCATAACTCATGATGTGTTTGTACCTCTAAAATGTCTTAGGACATTAAATTTGAGTGATTTCTGGTTCAAAGAACTACCGAAGGAGATTGGTGAGTTTGTGCATTTGAGATATCTCAATTTATCTGGTAATGACGAGTTGAAGATATTGCCTGAGACACTGTGTGAGCTGTCTAACTTGCAAACCTTGTTGGTGAGTAAGTGCATGCAACTGGAAAAACTTCCAGACCACATGGAGAAACTTGTTAGCTTAAGACATCTTCATGTTGATGGGTCTTTTAATCTATTGACATTACCAAAAGGAATTGCGAAGCTAGCTTCTCTTCAGACCCTAGACATGCTTGTTGTACCACCTTCTGACAATCTGCAAGCATTTCAGCTTGAAGATACGAAAAAGTTGAACAACGTTGAAGGGTATCTTCGTATCTGCAGGTGTGGAAATCTGAGAAATGTGCACGAGGCTGAAAATGCACAACTGTTTTGTAGAAGGCAGATTGTTGATTTGAATTTAGATTTTAGTTGGAGCCAAGGCCGGAGACTCAAAGATGAAATAGTACTTGAAGCCTTACGTCCACAGCCCAATTTGGAATCTTTAGAAATTCGGAAGTACAGAGGCACCACCATCTCCCCACATTGTTTATTAGTTTATATGAATGCGGTAATGTTTCAAAAGGTGATGCCTCTGGTTTCAGAGAATGAGCTGAAATCTCTATTGGCTCTTGGGTTGATAAGGAAGGATGTTCTTCATAGTGGTCAAGCAACAGCGCACTATAGGCCTCCTTCTGGGGTGCTTTTAAGCTTACTTGTGATGGGGCCACCGCGAGCTTCCTCTTATGACTGTTGGGTGGGGAAGTTGTGCACGATGAGGAAGAAAGTTTTATTTATGCATTTGGCCGGTGACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

847

Amino Acids

96.42

Weight (kDa)

6.11

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 17 - 79 2.5e-10 Rx N-terminal domain
NB-ARC PF00931 159 - 326 2.3e-35 NB-ARC domain
WHD_DRP PF23559 413 - 484 3.3e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 555 - 754 1.1e-16 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 673 - 781 5.8e-16 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021196)

Species Orthologous Gene IDs
malus_domestica MD17G1224300.v1.1
prunus_persica Prupe.3G100000_v2.0.a1
pyrus_communis pycom17g22800
rosa_laevigata RLG00000019277 RLG00000019281

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1415
AarI CACCTGC 1 cut(s) 2055
AasI GACNNNNNNGTC 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 2055
Acc65I GGTACC 1 cut(s) 1541
AccB1I GGYRCC 3 cut(s) 520, 1541, 2249
AccI GTMKAC 1 cut(s) 453
AccII CGCG 2 cut(s) 95, 2455
AciI CCGC 6 cut(s) 93, 518, 1052, 1317, 2291, 2453
AclI AACGTT 3 cut(s) 885, 1216, 2040
AclWI GGATC 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 2526
AcsI RAATTY 6 cut(s) 343, 858, 1385, 1684, 2140, 2232
AcuI CTGAAG 1 cut(s) 1943
AdeI CACNNNGTG 1 cut(s) 1799
AfaI GTAC 7 cut(s) 249, 1543, 1606, 1659, 1983, 2190, 2243
AfiI CCNNNNNNNGG 7 cut(s) 699, 741, 742, 810, 1538, 2417, 2535
AflII CTTAAG 1 cut(s) 1885
AflIII ACRYGT 1 cut(s) 174
AhdI GACNNNNNGTC 1 cut(s) 727
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 2 cut(s) 947, 1380
AjuI GAANNNNNNNTTGG 2 cut(s) 2330, 2362
AloI GAACNNNNNNTCC 1 cut(s) 32
Alw21I GWGCWC 5 cut(s) 13, 147, 1625, 2091, 2495
Alw26I GTCTC 7 cut(s) 109, 409, 497, 643, 1256, 1784, 2164
Alw44I GTGCAC 2 cut(s) 2087, 2491
AlwI GGATC 1 cut(s) 291
AoxI GGCC 6 cut(s) 105, 1449, 2164, 2408, 2447, 2526
ApaLI GTGCAC 2 cut(s) 2087, 2491
ApeKI GCWGC 2 cut(s) 1111, 1634
ApoI RAATTY 6 cut(s) 343, 858, 1385, 1684, 2140, 2232
ArsI GACNNNNNNTTYG 2 cut(s) 1669, 1701
Asp700I GAANNNNTTC 2 cut(s) 1476, 1854
Asp718I GGTACC 1 cut(s) 1541
AspLEI GCGC 2 cut(s) 97, 2401
AspS9I GGNCC 3 cut(s) 1086, 1450, 2447
AsuC2I CCSGG 2 cut(s) 710, 736
AsuHPI GGTGA 4 cut(s) 707, 1739, 1840, 2320
AsuNHI GCTAGC 1 cut(s) 1948
AvaII GGWCC 1 cut(s) 1086
BaeGI GKGCMC 4 cut(s) 132, 525, 2091, 2495
BaeI ACNNNNGTAYC 2 cut(s) 1533, 1566
BanI GGYRCC 3 cut(s) 520, 1541, 2249
BanII GRGCYC 1 cut(s) 147
BauI CACGAG 1 cut(s) 2090
BbsI GAAGAC 4 cut(s) 723, 804, 1017, 1070
Bbv12I GWGCWC 5 cut(s) 13, 147, 1625, 2091, 2495
BbvI GCAGC 2 cut(s) 1098, 1621
BccI CCATC 7 cut(s) 541, 804, 892, 1002, 1898, 2264, 2437
BceAI ACGGC 1 cut(s) 285
BciT130I CCWGG 2 cut(s) 949, 1382
BcnI CCSGG 2 cut(s) 710, 736
BcoDI GTCTC 7 cut(s) 109, 409, 497, 643, 1256, 1784, 2164
BfaI CTAG 4 cut(s) 779, 891, 1949, 1967
BfmI CTRYAG 2 cut(s) 2062, 2404
BfrI CTTAAG 1 cut(s) 1885
BfuAI ACCTGC 1 cut(s) 2055
BglII AGATCT 2 cut(s) 1075, 1370
BisI GCNGC 4 cut(s) 93, 1052, 1112, 1635
BlsI GCNGC 4 cut(s) 94, 1053, 1113, 1636
BmcAI AGTACT 1 cut(s) 2190
Bme1390I CCNGG 4 cut(s) 710, 736, 949, 1382
Bme18I GGWCC 1 cut(s) 1086
BmeRI GACNNNNNGTC 1 cut(s) 727
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 3 cut(s) 1086, 1450, 2447
BmiI GGNNCC 6 cut(s) 522, 1203, 1543, 2159, 2251, 2448
BmrFI CCNGG 4 cut(s) 710, 736, 949, 1382
BmrI ACTGGG 1 cut(s) 1447
BmsI GCATC 5 cut(s) 18, 1373, 1409, 1416, 2301
BmtI GCTAGC 1 cut(s) 1952
BmuI ACTGGG 1 cut(s) 1447
BpiI GAAGAC 4 cut(s) 723, 804, 1017, 1070
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 3 cut(s) 119, 299, 790
BpuMI CCSGG 2 cut(s) 710, 736
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 6 cut(s) 76, 108, 936, 1068, 1537, 2161
BsaWI WCCGGW 2 cut(s) 272, 692
BsaXI ACNNNNNCTCC 3 cut(s) 30, 161, 191
Bsc4I CCNNNNNNNGG 7 cut(s) 699, 741, 742, 810, 1538, 2417, 2535
Bse118I RCCGGY 1 cut(s) 2528
Bse1I ACTGG 5 cut(s) 137, 760, 1453, 1851, 2540
Bse3DI GCAATG 1 cut(s) 1594
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 2 cut(s) 949, 1382
BseDI CCNNGG 6 cut(s) 76, 108, 936, 1068, 1537, 2161
BseGI GGATG 6 cut(s) 145, 187, 552, 991, 1293, 2377
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 7 cut(s) 699, 741, 742, 810, 1538, 2417, 2535
BseMI GCAATG 1 cut(s) 1594
BseMII CTCAG 6 cut(s) 160, 492, 498, 654, 1779, 2069
BseNI ACTGG 5 cut(s) 137, 760, 1453, 1851, 2540
BseRI GAGGAG 3 cut(s) 116, 125, 793
BseSI GKGCMC 4 cut(s) 132, 525, 2091, 2495
BseXI GCAGC 2 cut(s) 1098, 1621
Bsh1236I CGCG 2 cut(s) 95, 2455
Bsh1285I CGRYCG 2 cut(s) 460, 603
BshFI GGCC 6 cut(s) 107, 1451, 2166, 2410, 2449, 2528
BshNI GGYRCC 3 cut(s) 520, 1541, 2249
BsiEI CGRYCG 2 cut(s) 460, 603
BsiHKAI GWGCWC 5 cut(s) 13, 147, 1625, 2091, 2495
BsiSI CCGG 6 cut(s) 273, 693, 710, 736, 2167, 2529
BslFI GGGAC 2 cut(s) 488, 758
BslI CCNNNNNNNGG 7 cut(s) 699, 741, 742, 810, 1538, 2417, 2535
BsmAI GTCTC 7 cut(s) 109, 409, 497, 643, 1256, 1784, 2164
BsmBI CGTCTC 2 cut(s) 109, 643
BsmFI GGGAC 2 cut(s) 488, 758
BsmI GAATGC 2 cut(s) 1478, 2293
BsnI GGCC 6 cut(s) 107, 1451, 2166, 2410, 2449, 2528
Bsp1286I GDGCHC 7 cut(s) 13, 132, 147, 525, 1625, 2091, 2495
Bsp143I GATC 4 cut(s) 283, 616, 1075, 1370
Bsp19I CCATGG 1 cut(s) 1537
BspACI CCGC 6 cut(s) 93, 518, 1052, 1317, 2291, 2453
BspANI GGCC 6 cut(s) 107, 1451, 2166, 2410, 2449, 2528
BspCNI CTCAG 6 cut(s) 159, 493, 499, 653, 1780, 2070
BspFNI CGCG 2 cut(s) 95, 2455
BspHI TCATGA 1 cut(s) 1645
BspLI GGNNCC 6 cut(s) 522, 1203, 1543, 2159, 2251, 2448
BspMAI CTGCAG 1 cut(s) 2066
BspMI ACCTGC 1 cut(s) 2055
BspOI GCTAGC 1 cut(s) 1952
BspPI GGATC 1 cut(s) 291
BspT107I GGYRCC 3 cut(s) 520, 1541, 2249
BspTI CTTAAG 1 cut(s) 1885
BsrDI GCAATG 1 cut(s) 1594
BsrFI RCCGGY 1 cut(s) 2528
BsrI ACTGG 5 cut(s) 137, 760, 1453, 1851, 2540
BssAI RCCGGY 1 cut(s) 2528
BssECI CCNNGG 6 cut(s) 76, 108, 936, 1068, 1537, 2161
BssMI GATC 4 cut(s) 283, 616, 1075, 1370
BssNAI GTATAC 1 cut(s) 454
BssSI CACGAG 1 cut(s) 2090
BssT1I CCWWGG 4 cut(s) 76, 1068, 1537, 2161
Bst1107I GTATAC 1 cut(s) 454
Bst2BI CACGAG 1 cut(s) 2090
Bst2UI CCWGG 2 cut(s) 949, 1382
Bst4CI ACNGT 9 cut(s) 252, 458, 601, 1134, 1232, 1271, 1797, 2112, 2474
Bst6I CTCTTC 2 cut(s) 117, 1962
BstAFI CTTAAG 1 cut(s) 1885
BstC8I GCNNGC 7 cut(s) 1033, 1049, 1056, 1841, 1950, 2004, 2457
BstDEI CTNAG 8 cut(s) 146, 501, 507, 640, 1435, 1673, 1788, 2078
BstDSI CCRYGG 1 cut(s) 1537
BstF5I GGATG 6 cut(s) 145, 187, 552, 991, 1293, 2377
BstFNI CGCG 2 cut(s) 95, 2455
BstHHI GCGC 2 cut(s) 97, 2401
BstKTI GATC 4 cut(s) 286, 619, 1078, 1373
BstMAI GTCTC 7 cut(s) 109, 409, 497, 643, 1256, 1784, 2164
BstMBI GATC 4 cut(s) 283, 616, 1075, 1370
BstMCI CGRYCG 2 cut(s) 460, 603
BstMWI GCNNNNNNNGC 5 cut(s) 89, 581, 1949, 2102, 2407
BstNI CCWGG 2 cut(s) 949, 1382
BstNSI RCATGY 4 cut(s) 1051, 1587, 1843, 1975
BstSCI CCNGG 4 cut(s) 708, 734, 947, 1380
BstSFI CTRYAG 2 cut(s) 2062, 2404
BstSLI GKGCMC 4 cut(s) 132, 525, 2091, 2495
BstUI CGCG 2 cut(s) 95, 2455
BstV1I GCAGC 2 cut(s) 1098, 1621
BstV2I GAAGAC 4 cut(s) 723, 804, 1017, 1070
BstX2I RGATCY 2 cut(s) 1075, 1370
BstYI RGATCY 2 cut(s) 1075, 1370
BstZ17I GTATAC 1 cut(s) 454
BsuRI GGCC 6 cut(s) 107, 1451, 2166, 2410, 2449, 2528
BtgI CCRYGG 1 cut(s) 1537
BtgZI GCGATG 1 cut(s) 281
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 6 cut(s) 145, 187, 552, 991, 1293, 2377
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 3 cut(s) 130, 192, 1793
BveI ACCTGC 1 cut(s) 2055
Cac8I GCNNGC 7 cut(s) 1033, 1049, 1056, 1841, 1950, 2004, 2457
CciI TCATGA 1 cut(s) 1645
CfoI GCGC 2 cut(s) 97, 2401
Cfr10I RCCGGY 1 cut(s) 2528
Cfr13I GGNCC 3 cut(s) 1086, 1450, 2447
CseI GACGC 1 cut(s) 625
Csp6I GTAC 7 cut(s) 248, 1542, 1605, 1658, 1982, 2189, 2242
CspCI CAANNNNNGTGG 2 cut(s) 1164, 1199
CviQI GTAC 7 cut(s) 248, 1542, 1605, 1658, 1982, 2189, 2242
DdeI CTNAG 8 cut(s) 146, 501, 507, 640, 1435, 1673, 1788, 2078
DpnI GATC 4 cut(s) 285, 618, 1077, 1372
DpnII GATC 4 cut(s) 283, 616, 1075, 1370
DraIII CACNNNGTG 1 cut(s) 1799
DrdI GACNNNNNNGTC 1 cut(s) 599
DriI GACNNNNNGTC 1 cut(s) 727
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 1 cut(s) 2526
Eam1104I CTCTTC 2 cut(s) 117, 1962
Eam1105I GACNNNNNGTC 1 cut(s) 727
EarI CTCTTC 2 cut(s) 117, 1962
Ecl136II GAGCTC 1 cut(s) 145
Eco130I CCWWGG 4 cut(s) 76, 1068, 1537, 2161
Eco147I AGGCCT 1 cut(s) 2410
Eco24I GRGCYC 1 cut(s) 147
Eco32I GATATC 1 cut(s) 1748
Eco47I GGWCC 1 cut(s) 1086
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 1943
EcoICRI GAGCTC 1 cut(s) 145
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 2 cut(s) 947, 1380
EcoRV GATATC 1 cut(s) 1748
EcoT14I CCWWGG 4 cut(s) 76, 1068, 1537, 2161
EcoT22I ATGCAT 2 cut(s) 1431, 2523
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 4 cut(s) 76, 1068, 1537, 2161
Esp3I CGTCTC 2 cut(s) 109, 643
FaqI GGGAC 2 cut(s) 488, 758
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 4 cut(s) 93, 1052, 1112, 1635
FokI GGATG 6 cut(s) 152, 194, 559, 998, 1300, 2384
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 4 cut(s) 93, 1052, 1112, 1635
FspBI CTAG 4 cut(s) 779, 891, 1949, 1967
GlaI GCGC 2 cut(s) 96, 2400
GluI GCNGC 4 cut(s) 93, 1052, 1112, 1635
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 6 cut(s) 107, 1451, 2166, 2410, 2449, 2528
HapII CCGG 6 cut(s) 273, 693, 710, 736, 2167, 2529
HgaI GACGC 1 cut(s) 625
HhaI GCGC 2 cut(s) 97, 2401
Hin6I GCGC 2 cut(s) 95, 2399
HinP1I GCGC 2 cut(s) 95, 2399
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 2431
HpaII CCGG 6 cut(s) 273, 693, 710, 736, 2167, 2529
HphI GGTGA 4 cut(s) 707, 1739, 1840, 2320
Hpy166II GTNNAC 6 cut(s) 403, 454, 1130, 2089, 2207, 2493
Hpy188III TCNNGA 8 cut(s) 316, 461, 680, 807, 1304, 1368, 1646, 1859
Hpy8I GTNNAC 6 cut(s) 403, 454, 1130, 2089, 2207, 2493
Hpy99I CGWCG 1 cut(s) 536
HpyCH4III ACNGT 9 cut(s) 252, 458, 601, 1134, 1232, 1271, 1797, 2112, 2474
HpyCH4IV ACGT 6 cut(s) 174, 626, 885, 1216, 2040, 2203
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 581, 1949, 2102, 2407
HpyF3I CTNAG 8 cut(s) 146, 501, 507, 640, 1435, 1673, 1788, 2078
HpySE526I ACGT 6 cut(s) 174, 626, 885, 1216, 2040, 2203
HspAI GCGC 2 cut(s) 95, 2399
KpnI GGTACC 1 cut(s) 1545
Kzo9I GATC 4 cut(s) 283, 616, 1075, 1370
LmnI GCTCC 5 cut(s) 8, 80, 1207, 1628, 2157
Lsp1109I GCAGC 2 cut(s) 1098, 1621
LweI GCATC 5 cut(s) 18, 1373, 1409, 1416, 2301
MaeI CTAG 4 cut(s) 779, 891, 1949, 1967
MaeII ACGT 6 cut(s) 174, 626, 885, 1216, 2040, 2203
MaeIII GTNAC 5 cut(s) 865, 1187, 1290, 1459, 2531
MalI GATC 4 cut(s) 285, 618, 1077, 1372
MboI GATC 4 cut(s) 283, 616, 1075, 1370
MflI RGATCY 2 cut(s) 1075, 1370
MhlI GDGCHC 7 cut(s) 13, 132, 147, 525, 1625, 2091, 2495
MlyI GAGTC 7 cut(s) 242, 340, 716, 740, 794, 1527, 2166
MmeI TCCRAC 4 cut(s) 117, 159, 829, 2135
Mph1103I ATGCAT 2 cut(s) 1431, 2523
MroXI GAANNNNTTC 2 cut(s) 1476, 1854
MseI TTAA 9 cut(s) 65, 155, 209, 438, 1278, 1682, 1886, 1914, 2429
MslI CAYNNNNRTG 3 cut(s) 518, 1896, 1902
MspA1I CMGCKG 2 cut(s) 1317, 1559
MspCI CTTAAG 1 cut(s) 1885
MspI CCGG 6 cut(s) 273, 693, 710, 736, 2167, 2529
MspR9I CCNGG 4 cut(s) 710, 736, 949, 1382
Mva1269I GAATGC 2 cut(s) 1478, 2293
MvaI CCWGG 2 cut(s) 949, 1382
MvnI CGCG 2 cut(s) 95, 2455
MwoI GCNNNNNNNGC 5 cut(s) 89, 581, 1949, 2102, 2407
NciI CCSGG 2 cut(s) 710, 736
NcoI CCATGG 1 cut(s) 1537
NdeII GATC 4 cut(s) 283, 616, 1075, 1370
NheI GCTAGC 1 cut(s) 1948
NlaIV GGNNCC 6 cut(s) 522, 1203, 1543, 2159, 2251, 2448
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 1290, 2531
NsiI ATGCAT 2 cut(s) 1431, 2523
NspI RCATGY 4 cut(s) 1051, 1587, 1843, 1975
PaeI GCATGC 2 cut(s) 1051, 1843
PagI TCATGA 1 cut(s) 1645
PaqCI CACCTGC 1 cut(s) 2055
PceI AGGCCT 1 cut(s) 2410
PctI GAATGC 2 cut(s) 1478, 2293
PdmI GAANNNNTTC 2 cut(s) 1476, 1854
PfeI GAWTC 5 cut(s) 375, 391, 944, 1307, 2222
PfoI TCCNGGA 2 cut(s) 734, 1380
PkrI GCNGC 4 cut(s) 94, 1053, 1113, 1636
PleI GAGTC 7 cut(s) 241, 339, 716, 740, 794, 1526, 2166
PpsI GAGTC 7 cut(s) 241, 339, 716, 740, 794, 1526, 2166
PsiI TTATAA 1 cut(s) 1415
Psp124BI GAGCTC 1 cut(s) 147
Psp1406I AACGTT 3 cut(s) 885, 1216, 2040
Psp6I CCWGG 2 cut(s) 947, 1380
PspGI CCWGG 2 cut(s) 947, 1380
PspN4I GGNNCC 6 cut(s) 522, 1203, 1543, 2159, 2251, 2448
PspPI GGNCC 3 cut(s) 1086, 1450, 2447
PstI CTGCAG 1 cut(s) 2066
PsuI RGATCY 2 cut(s) 1075, 1370
PvuII CAGCTG 1 cut(s) 1559
RsaI GTAC 7 cut(s) 249, 1543, 1606, 1659, 1983, 2190, 2243
RsaNI GTAC 7 cut(s) 248, 1542, 1605, 1658, 1982, 2189, 2242
RseI CAYNNNNRTG 3 cut(s) 518, 1896, 1902
SacI GAGCTC 1 cut(s) 147
SaqAI TTAA 9 cut(s) 65, 155, 209, 438, 1278, 1682, 1886, 1914, 2429
SatI GCNGC 4 cut(s) 93, 1052, 1112, 1635
Sau3AI GATC 4 cut(s) 283, 616, 1075, 1370
Sau96I GGNCC 3 cut(s) 1086, 1450, 2447
ScaI AGTACT 1 cut(s) 2190
SchI GAGTC 7 cut(s) 242, 340, 716, 740, 794, 1527, 2166
ScrFI CCNGG 4 cut(s) 710, 736, 949, 1382
SduI GDGCHC 7 cut(s) 13, 132, 147, 525, 1625, 2091, 2495
SfaNI GCATC 5 cut(s) 18, 1373, 1409, 1416, 2301
SfcI CTRYAG 2 cut(s) 2062, 2404
SinI GGWCC 1 cut(s) 1086
SmiMI CAYNNNNRTG 3 cut(s) 518, 1896, 1902
SmlI CTYRAG 4 cut(s) 98, 314, 805, 1885
SmoI CTYRAG 4 cut(s) 98, 314, 805, 1885
SphI GCATGC 2 cut(s) 1051, 1843
SseBI AGGCCT 1 cut(s) 2410
SsiI CCGC 6 cut(s) 93, 518, 1052, 1317, 2291, 2453
SspI AATATT 1 cut(s) 1411
SspMI CTAG 4 cut(s) 779, 891, 1949, 1967
SstI GAGCTC 1 cut(s) 147
StuI AGGCCT 1 cut(s) 2410
StyD4I CCNGG 4 cut(s) 708, 734, 947, 1380
StyI CCWWGG 4 cut(s) 76, 1068, 1537, 2161
TaaI ACNGT 9 cut(s) 252, 458, 601, 1134, 1232, 1271, 1797, 2112, 2474
TaiI ACGT 6 cut(s) 177, 629, 888, 1219, 2043, 2206
TaqI TCGA 3 cut(s) 286, 460, 609
TatI WGTACW 2 cut(s) 2188, 2241
TauI GCSGC 2 cut(s) 95, 1054
TfiI GAWTC 5 cut(s) 375, 391, 944, 1307, 2222
Tru1I TTAA 9 cut(s) 65, 155, 209, 438, 1278, 1682, 1886, 1914, 2429
Tru9I TTAA 9 cut(s) 65, 155, 209, 438, 1278, 1682, 1886, 1914, 2429
TscAI CASTG 3 cut(s) 137, 199, 1800
TseFI GTSAC 2 cut(s) 1290, 2531
TseI GCWGC 2 cut(s) 1111, 1634
Tsp45I GTSAC 2 cut(s) 1290, 2531
TspRI CASTG 3 cut(s) 137, 199, 1800
Vha464I CTTAAG 1 cut(s) 1885
VneI GTGCAC 2 cut(s) 2087, 2491
VpaK11BI GGWCC 1 cut(s) 1086
XapI RAATTY 6 cut(s) 343, 858, 1385, 1684, 2140, 2232
XceI RCATGY 4 cut(s) 1051, 1587, 1843, 1975
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 2 cut(s) 1476, 1854
XspI CTAG 4 cut(s) 779, 891, 1949, 1967
ZrmI AGTACT 1 cut(s) 2190
Zsp2I ATGCAT 2 cut(s) 1431, 2523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.