MD17G1250400.v1.1

Lipid phosphate phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007586
Physical Location & Seq
Forward (+)
30000208 .. 30004227
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1250400.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGCCTTGGTGGGATCTTGGATTGCTTTCTCGTCTCAAAAACTTGAGTGGCGTCGTCTTTCAGAACAAAATGCAGGAAGTTGAGCTTGGTTCACATACTGTAAGGTCCCATGGAGTGACAGTTGCAAGGACACACATGCATGATTGGGTGATACTGATGCTTCTTGGGTTGATAGAGGTCATCCTTTATGTGATTCATCCATTTTATCGCTTCGTAGGAAAAGATATGATGACTGATCTTAAATATCCCTTGAAAAGTTGCACAGTACCTTTTTGGGCTGTCCCTATGTATGCAGTTGCACTGCCCATAGCAATTTTTGTTGTTGTCTATTTCCGCAGGAGAGATGTCTATGACCTTCACCATGCCATACTAGGTCTATTGTTCTCTGTTCTGATAACAGGGGTTCTTACAGATGCTATAAAAGATGCAGTTGGTCGACCCCGACCAGACTTCTTTTGGCGCTGTTTTCCAGATGGGAAGGATGTGTATGATAAATGGGGAAATGTTGTATGCTATGGTGTGAAGAGTGTTATAAAGGAGGGGCACAAGAGTTTTCCAAGTGGCCATACTTCATGGTCCTTTGCTGGTCTTGGATTTTTCTCACTGTATTTGTCAGGAAAAATACAAGTATTTGATCGCAAAGGCCATGTTGCAAAACTTTGCATTGTTATTCTTCCACTACTTGTTGCATCACTTGTCGGAGTTTCTCTAGTAGATGACTACTGGCACCACTGGCAAGATGTGTTCGCTGGAGGTCTCCTAGGCCTTACGGTTGCCACCTTTTGCTATTTGAAGTTCTTTCCACCCCCATACCATGCCCAAGGTTGGGGCCCTTATGCTTACTTCCAAGTGTTGGAGGAATTGCGTTCAGGGGCACAAGTAACCAGCACAATAAATGGGAGCAACGTGCAGGTCACAGAGGCTCGGGTTGAGAATCAAGTAGACGAAACAAGCAACCATGGATGTATGGGGTTAACATTAGCACGTGGTTCTGGTTCAGCGTTGGAAGATTTAGAATCTGGGAGGAGATAG

Protein Analysis

344

Amino Acids

38.53

Weight (kDa)

8.16

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 123 - 266 3.4e-29 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 901
AccB1I GGYRCC 1 cut(s) 726
AccB7I CCANNNNNTGG 1 cut(s) 853
AccI GTMKAC 2 cut(s) 436, 942
AciI CCGC 1 cut(s) 334
AclWI GGATC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 562
AcvI CACGTG 1 cut(s) 986
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 3 cut(s) 825, 826, 853
AgsI TTSAA 2 cut(s) 253, 793
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 1 cut(s) 85
AluI AGCT 1 cut(s) 85
Alw26I GTCTC 2 cut(s) 38, 761
AlwI GGATC 1 cut(s) 21
Ama87I CYCGRG 1 cut(s) 924
AoxI GGCC 4 cut(s) 562, 643, 763, 829
ApaI GGGCCC 1 cut(s) 833
AspA2I CCTAGG 1 cut(s) 760
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 4 cut(s) 105, 576, 829, 830
AsuHPI GGTGA 2 cut(s) 160, 350
AvaI CYCGRG 1 cut(s) 924
AvaII GGWCC 2 cut(s) 105, 576
AvrII CCTAGG 1 cut(s) 760
BaeGI GKGCMC 3 cut(s) 546, 833, 877
BalI TGGCCA 1 cut(s) 564
BanI GGYRCC 1 cut(s) 726
BanII GRGCYC 1 cut(s) 833
BbrPI CACGTG 1 cut(s) 986
BccI CCATC 1 cut(s) 467
BcoDI GTCTC 2 cut(s) 38, 761
BfaI CTAG 3 cut(s) 371, 710, 761
BfoI RGCGCY 1 cut(s) 463
BfuAI ACCTGC 1 cut(s) 901
BlnI CCTAGG 1 cut(s) 760
Bme18I GGWCC 2 cut(s) 105, 576
BmeT110I CYCGRG 1 cut(s) 924
BmgT120I GGNCC 4 cut(s) 105, 576, 829, 830
BmiI GGNNCC 4 cut(s) 107, 728, 830, 831
BmsI GCATC 4 cut(s) 147, 403, 415, 698
BpmI CTGGAG 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 986
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 761
BsaJI CCNNGG 5 cut(s) 5, 109, 760, 820, 958
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 3 cut(s) 825, 826, 853
Bse1I ACTGG 2 cut(s) 728, 737
BseDI CCNNGG 5 cut(s) 5, 109, 760, 820, 958
BseGI GGATG 4 cut(s) 180, 196, 487, 968
BseLI CCNNNNNNNGG 3 cut(s) 825, 826, 853
BseNI ACTGG 2 cut(s) 728, 737
BseSI GKGCMC 3 cut(s) 546, 833, 877
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 4 cut(s) 564, 645, 765, 831
BshNI GGYRCC 1 cut(s) 726
BsiHKCI CYCGRG 1 cut(s) 924
BslFI GGGAC 2 cut(s) 91, 266
BslI CCNNNNNNNGG 3 cut(s) 825, 826, 853
BsmAI GTCTC 2 cut(s) 38, 761
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 2 cut(s) 91, 266
BsnI GGCC 4 cut(s) 564, 645, 765, 831
Bso31I GGTCTC 1 cut(s) 761
BsoBI CYCGRG 1 cut(s) 924
Bsp120I GGGCCC 1 cut(s) 829
Bsp1286I GDGCHC 3 cut(s) 546, 833, 877
Bsp143I GATC 3 cut(s) 13, 235, 634
Bsp19I CCATGG 2 cut(s) 109, 958
BspACI CCGC 1 cut(s) 334
BspANI GGCC 4 cut(s) 564, 645, 765, 831
BspLI GGNNCC 4 cut(s) 107, 728, 830, 831
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 1 cut(s) 21
BspT107I GGYRCC 1 cut(s) 726
BspTNI GGTCTC 1 cut(s) 761
BsrI ACTGG 2 cut(s) 728, 737
BssECI CCNNGG 5 cut(s) 5, 109, 760, 820, 958
BssMI GATC 3 cut(s) 13, 235, 634
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 5 cut(s) 5, 109, 760, 820, 958
Bst4CI ACNGT 5 cut(s) 100, 121, 265, 606, 772
Bst6I CTCTTC 1 cut(s) 518
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 986
BstDSI CCRYGG 2 cut(s) 109, 958
BstF5I GGATG 4 cut(s) 180, 196, 487, 968
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 3 cut(s) 16, 238, 637
BstMAI GTCTC 2 cut(s) 38, 761
BstMBI GATC 3 cut(s) 13, 235, 634
BstMWI GCNNNNNNNGC 1 cut(s) 733
BstNSI RCATGY 1 cut(s) 139
BstSLI GKGCMC 3 cut(s) 546, 833, 877
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 4 cut(s) 564, 645, 765, 831
BtgI CCRYGG 2 cut(s) 109, 958
BtsCI GGATG 4 cut(s) 180, 196, 487, 968
BtsI GCAGTG 1 cut(s) 299
BtsIMutI CAGTG 3 cut(s) 299, 602, 730
BveI ACCTGC 1 cut(s) 901
CfoI GCGC 1 cut(s) 462
Cfr13I GGNCC 4 cut(s) 105, 576, 829, 830
CseI GACGC 1 cut(s) 40
Csp6I GTAC 1 cut(s) 266
CviAII CATG 8 cut(s) 110, 136, 140, 362, 573, 647, 815, 959
CviJI RGCY 7 cut(s) 85, 278, 564, 645, 765, 831, 923
CviKI_1 RGCY 7 cut(s) 85, 278, 564, 645, 765, 831, 923
CviQI GTAC 1 cut(s) 266
DpnI GATC 3 cut(s) 15, 237, 636
DpnII GATC 3 cut(s) 13, 235, 634
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 518
Eco130I CCWWGG 5 cut(s) 5, 109, 760, 820, 958
Eco147I AGGCCT 1 cut(s) 765
Eco24I GRGCYC 1 cut(s) 833
Eco31I GGTCTC 1 cut(s) 761
Eco47I GGWCC 2 cut(s) 105, 576
Eco72I CACGTG 1 cut(s) 986
Eco88I CYCGRG 1 cut(s) 924
EcoO109I RGGNCCY 3 cut(s) 105, 829, 830
EcoT14I CCWWGG 5 cut(s) 5, 109, 760, 820, 958
EcoT22I ATGCAT 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 833
ErhI CCWWGG 5 cut(s) 5, 109, 760, 820, 958
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 8 cut(s) 113, 139, 143, 365, 576, 650, 818, 962
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 2 cut(s) 91, 266
FatI CATG 8 cut(s) 109, 135, 139, 361, 572, 646, 814, 958
FblI GTMKAC 2 cut(s) 436, 942
FokI GGATG 4 cut(s) 167, 183, 494, 975
FriOI GRGCYC 1 cut(s) 833
FspBI CTAG 3 cut(s) 371, 710, 761
GlaI GCGC 1 cut(s) 461
GsuI CTGGAG 1 cut(s) 771
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 4 cut(s) 564, 645, 765, 831
HgaI GACGC 1 cut(s) 40
HhaI GCGC 1 cut(s) 462
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 8 cut(s) 113, 139, 143, 365, 576, 650, 818, 962
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HincII GTYRAC 2 cut(s) 437, 975
HindII GTYRAC 2 cut(s) 437, 975
HinfI GANTC 3 cut(s) 193, 934, 1016
HpaI GTTAAC 1 cut(s) 975
HphI GGTGA 2 cut(s) 160, 350
Hpy166II GTNNAC 4 cut(s) 92, 437, 943, 975
Hpy188I TCNGA 3 cut(s) 63, 393, 701
Hpy188III TCNNGA 2 cut(s) 470, 615
Hpy8I GTNNAC 4 cut(s) 92, 437, 943, 975
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 2 cut(s) 365, 472
HpyCH4III ACNGT 5 cut(s) 100, 121, 265, 606, 772
HpyCH4IV ACGT 2 cut(s) 906, 985
HpyF10VI GCNNNNNNNGC 1 cut(s) 733
HpySE526I ACGT 2 cut(s) 906, 985
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 8 cut(s) 113, 139, 143, 365, 576, 650, 818, 962
HspAI GCGC 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 975
Kzo9I GATC 3 cut(s) 13, 235, 634
LmnI GCTCC 1 cut(s) 900
LweI GCATC 4 cut(s) 147, 403, 415, 698
MaeI CTAG 3 cut(s) 371, 710, 761
MaeII ACGT 2 cut(s) 906, 985
MaeIII GTNAC 3 cut(s) 115, 880, 913
MalI GATC 3 cut(s) 15, 237, 636
MboI GATC 3 cut(s) 13, 235, 634
MboII GAAGA 3 cut(s) 535, 665, 1019
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 3 cut(s) 546, 833, 877
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 2 cut(s) 312, 860
MluNI TGGCCA 1 cut(s) 564
MmeI TCCRAC 3 cut(s) 679, 834, 984
MnlI CCTC 6 cut(s) 169, 532, 746, 850, 913, 1017
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 141
MscI TGGCCA 1 cut(s) 564
MseI TTAA 2 cut(s) 240, 974
MslI CAYNNNNRTG 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 564
MwoI GCNNNNNNNGC 1 cut(s) 733
NcoI CCATGG 2 cut(s) 109, 958
NdeII GATC 3 cut(s) 13, 235, 634
NlaIII CATG 8 cut(s) 113, 139, 143, 365, 576, 650, 818, 962
NlaIV GGNNCC 4 cut(s) 107, 728, 830, 831
NmuCI GTSAC 2 cut(s) 115, 913
NsiI ATGCAT 1 cut(s) 141
NspI RCATGY 1 cut(s) 139
PceI AGGCCT 1 cut(s) 765
PfeI GAWTC 3 cut(s) 193, 934, 1016
PflMI CCANNNNNTGG 1 cut(s) 853
PmaCI CACGTG 1 cut(s) 986
PmlI CACGTG 1 cut(s) 986
Ppu21I YACGTR 1 cut(s) 986
PpuMI RGGWCCY 1 cut(s) 105
PsiI TTATAA 1 cut(s) 533
Psp5II RGGWCCY 1 cut(s) 105
PspCI CACGTG 1 cut(s) 986
PspN4I GGNNCC 4 cut(s) 107, 728, 830, 831
PspOMI GGGCCC 1 cut(s) 829
PspPI GGNCC 4 cut(s) 105, 576, 829, 830
PspPPI RGGWCCY 1 cut(s) 105
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
RseI CAYNNNNRTG 1 cut(s) 138
SalI GTCGAC 1 cut(s) 435
SaqAI TTAA 2 cut(s) 240, 974
Sau3AI GATC 3 cut(s) 13, 235, 634
Sau96I GGNCC 4 cut(s) 105, 576, 829, 830
SduI GDGCHC 3 cut(s) 546, 833, 877
SfaNI GCATC 4 cut(s) 147, 403, 415, 698
SinI GGWCC 2 cut(s) 105, 576
SmiMI CAYNNNNRTG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 43
SmoI CTYRAG 1 cut(s) 43
Sse9I AATT 2 cut(s) 312, 860
SseBI AGGCCT 1 cut(s) 765
SsiI CCGC 1 cut(s) 334
SspMI CTAG 3 cut(s) 371, 710, 761
StuI AGGCCT 1 cut(s) 765
StyI CCWWGG 5 cut(s) 5, 109, 760, 820, 958
TaaI ACNGT 5 cut(s) 100, 121, 265, 606, 772
TaiI ACGT 2 cut(s) 909, 988
TaqI TCGA 1 cut(s) 436
TasI AATT 2 cut(s) 312, 860
TfiI GAWTC 3 cut(s) 193, 934, 1016
Tru1I TTAA 2 cut(s) 240, 974
Tru9I TTAA 2 cut(s) 240, 974
TscAI CASTG 3 cut(s) 306, 609, 737
TseFI GTSAC 2 cut(s) 115, 913
Tsp45I GTSAC 2 cut(s) 115, 913
TspDTI ATGAA 2 cut(s) 185, 561
TspRI CASTG 3 cut(s) 306, 609, 737
Van91I CCANNNNNTGG 1 cut(s) 853
VpaK11BI GGWCC 2 cut(s) 105, 576
XceI RCATGY 1 cut(s) 139
XcmI CCANNNNNNNNNTGG 1 cut(s) 453
XmaJI CCTAGG 1 cut(s) 760
XmiI GTMKAC 2 cut(s) 436, 942
XspI CTAG 3 cut(s) 371, 710, 761
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.