MD17G1271900.v1.1

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008596
Physical Location & Seq
Forward (+)
33252122 .. 33253062
941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1271900.v1.1.491

Sequence Viewer

Length: 834 bp
ATGGAGGAAGATGACATTGTGGACGGCGATTTCTACCTCCTTGCTAAGTCGTATTTTGACTGCAGGGAGTACAGAAGAGCTGCTCATGTGCTTCGTGATCAGAACGGAAAGAAGCGGTCGAACTTTGAGGGGCCCCTAGGAAAGAGCGATATTGTGAATGGTGAACTGGTTTCTCTCGAGAGGGAGTTATCAACCCTTCGCAAGAATGGGACTATTGATCCTTTTGGACTCTACTTGTATGGCCTTGTGCTCAAAGACAAAGGCAGCAAGACTACCCTTGGAACTGGGAGTGCCTGGTCGGAGCTGCAGTCGTTGTGCACTACAGTTGATGTCTTGAACAGCCTTAAGCTCAATAACCATTGGATGAAGGACTTCTTTCTCGCCAGTGCTTATCAAGAACTGAGGAAGCACAGTGAATCCTTAAAACAATATGAATATCTTAAGGGCACTTTTGTTTTCAGTAATTACATTCAGGCTCAAATTGCAAAGGCACAATACAATCTCAGGGAATTTGAACAAGTTGAAGCAATTTTTGAAGAACTTCTCAGGAATGACCCTTATCGAGTGGAAGACATGGACATGTATTCCAATGTGCTATATGCAAAGGAGTGCTACTCTGCACTAAGTTATCTTGCCCATAGAGTATTTATCACTGATAAATACAGGCCTGAATCTTGTTGTATTATCGGAAATTACTACAGCTTAAAAGGGCAGCATGAGAAGGCGGTTGTGTACTTTAGGAGGGCACTCAAATTGAACAGAAACTATTTATCTGCTTGGACCCTTATGGGTCATGAATATGTTGAGATGAAAAAACACCCCAGCTGCTATTGA

Protein Analysis

278

Amino Acids

32.36

Weight (kDa)

7.04

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC8 PF04049 9 - 38 2.5e-08 Anaphase promoting complex subunit 8 / Cdc23
ANAPC8 PF04049 97 - 162 4.6e-12 Anaphase promoting complex subunit 8 / Cdc23
TPR_1 PF00515 224 - 256 2.3e-07 Tetratricopeptide repeat
TPR_8 PF13181 224 - 255 2.6e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 115, 725
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 1 cut(s) 509
AfaI GTAC 2 cut(s) 71, 734
AflII CTTAAG 2 cut(s) 344, 440
AflIII ACRYGT 1 cut(s) 579
AgsI TTSAA 5 cut(s) 337, 515, 524, 536, 757
AjnI CCWGG 1 cut(s) 293
AluBI AGCT 5 cut(s) 80, 304, 349, 702, 825
AluI AGCT 5 cut(s) 80, 304, 349, 702, 825
Alw21I GWGCWC 2 cut(s) 252, 320
Alw44I GTGCAC 1 cut(s) 316
AlwI GGATC 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 3 cut(s) 131, 241, 665
ApaI GGGCCC 1 cut(s) 135
ApaLI GTGCAC 1 cut(s) 316
ApeKI GCWGC 5 cut(s) 80, 264, 304, 712, 825
ApoI RAATTY 1 cut(s) 509
Asp700I GAANNNNTTC 2 cut(s) 371, 540
AspA2I CCTAGG 1 cut(s) 136
AspS9I GGNCC 3 cut(s) 131, 132, 780
AsuHPI GGTGA 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 780
AvrII CCTAGG 1 cut(s) 136
BaeGI GKGCMC 4 cut(s) 135, 320, 449, 748
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 576
Bbv12I GWGCWC 2 cut(s) 252, 320
BbvI GCAGC 5 cut(s) 67, 276, 291, 724, 812
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 295
BclI TGATCA 1 cut(s) 97
BfaI CTAG 1 cut(s) 137
BfmI CTRYAG 4 cut(s) 61, 305, 321, 697
BfrI CTTAAG 2 cut(s) 344, 440
BisI GCNGC 5 cut(s) 81, 265, 305, 713, 826
BlnI CCTAGG 1 cut(s) 136
BlsI GCNGC 5 cut(s) 82, 266, 306, 714, 827
Bme1390I CCNGG 1 cut(s) 295
Bme18I GGWCC 1 cut(s) 780
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 3 cut(s) 131, 132, 780
BmiI GGNNCC 4 cut(s) 132, 133, 134, 782
BmrFI CCNGG 1 cut(s) 295
BmrI ACTGGG 1 cut(s) 294
BmuI ACTGGG 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 576
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BsaJI CCNNGG 2 cut(s) 136, 277
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse1I ACTGG 3 cut(s) 171, 289, 384
BseBI CCWGG 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 136, 277
BseGI GGATG 1 cut(s) 369
BseMII CTCAG 3 cut(s) 392, 517, 559
BseNI ACTGG 3 cut(s) 171, 289, 384
BseSI GKGCMC 4 cut(s) 135, 320, 449, 748
BseXI GCAGC 5 cut(s) 67, 276, 291, 724, 812
BseYI CCCAGC 1 cut(s) 821
BsgI GTGCAG 1 cut(s) 603
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 133, 243, 667
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 252, 320
BsiHKCI CYCGRG 1 cut(s) 176
BslFI GGGAC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 3 cut(s) 133, 243, 667
BsoBI CYCGRG 1 cut(s) 176
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 5 cut(s) 135, 252, 320, 449, 748
Bsp143I GATC 2 cut(s) 97, 217
BspACI CCGC 2 cut(s) 115, 725
BspANI GGCC 3 cut(s) 133, 243, 667
BspCNI CTCAG 3 cut(s) 393, 516, 558
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 4 cut(s) 132, 133, 134, 782
BspMAI CTGCAG 2 cut(s) 65, 309
BspPI GGATC 1 cut(s) 212
BspQI GCTCTTC 1 cut(s) 70
BspTI CTTAAG 2 cut(s) 344, 440
BsrI ACTGG 3 cut(s) 171, 289, 384
BssECI CCNNGG 2 cut(s) 136, 277
BssMI GATC 2 cut(s) 97, 217
BssT1I CCWWGG 2 cut(s) 136, 277
Bst2UI CCWGG 1 cut(s) 295
Bst4CI ACNGT 2 cut(s) 325, 413
Bst6I CTCTTC 1 cut(s) 70
BstAFI CTTAAG 2 cut(s) 344, 440
BstDEI CTNAG 5 cut(s) 45, 401, 503, 545, 623
BstF5I GGATG 1 cut(s) 369
BstKTI GATC 2 cut(s) 100, 220
BstMBI GATC 2 cut(s) 97, 217
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 1 cut(s) 295
BstNSI RCATGY 1 cut(s) 583
BstSCI CCNGG 1 cut(s) 293
BstSFI CTRYAG 4 cut(s) 61, 305, 321, 697
BstSLI GKGCMC 4 cut(s) 135, 320, 449, 748
BstV1I GCAGC 5 cut(s) 67, 276, 291, 724, 812
BstV2I GAAGAC 1 cut(s) 576
BsuRI GGCC 3 cut(s) 133, 243, 667
BtsCI GGATG 1 cut(s) 369
BtsIMutI CAGTG 3 cut(s) 391, 418, 651
CciI TCATGA 1 cut(s) 793
Cfr13I GGNCC 3 cut(s) 131, 132, 780
Csp6I GTAC 2 cut(s) 70, 733
CviAII CATG 5 cut(s) 86, 574, 580, 716, 794
CviQI GTAC 2 cut(s) 70, 733
DdeI CTNAG 5 cut(s) 45, 401, 503, 545, 623
DpnI GATC 2 cut(s) 99, 219
DpnII GATC 2 cut(s) 97, 217
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco130I CCWWGG 2 cut(s) 136, 277
Eco147I AGGCCT 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 780
Eco88I CYCGRG 1 cut(s) 176
EcoO109I RGGNCCY 2 cut(s) 131, 132
EcoRII CCWGG 1 cut(s) 293
EcoT14I CCWWGG 2 cut(s) 136, 277
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 2 cut(s) 136, 277
FaeI CATG 5 cut(s) 89, 577, 583, 719, 797
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 1 cut(s) 223
FatI CATG 5 cut(s) 85, 573, 579, 715, 793
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 5 cut(s) 81, 265, 305, 713, 826
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 5 cut(s) 81, 265, 305, 713, 826
FspBI CTAG 1 cut(s) 137
GluI GCNGC 5 cut(s) 81, 265, 305, 713, 826
GsaI CCCAGC 1 cut(s) 825
HaeIII GGCC 3 cut(s) 133, 243, 667
Hin1II CATG 5 cut(s) 89, 577, 583, 719, 797
HinfI GANTC 3 cut(s) 228, 416, 671
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 4 cut(s) 22, 164, 318, 733
Hpy188I TCNGA 3 cut(s) 102, 301, 689
Hpy188III TCNNGA 7 cut(s) 95, 176, 178, 334, 395, 547, 794
Hpy8I GTNNAC 4 cut(s) 22, 164, 318, 733
HpyAV CCTTC 3 cut(s) 206, 361, 715
HpyCH4III ACNGT 2 cut(s) 325, 413
HpyCH4V TGCA 6 cut(s) 63, 307, 318, 485, 602, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 5 cut(s) 45, 401, 503, 545, 623
Hsp92II CATG 5 cut(s) 89, 577, 583, 719, 797
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 2 cut(s) 97, 217
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 301
Lsp1109I GCAGC 5 cut(s) 67, 276, 291, 724, 812
MaeI CTAG 1 cut(s) 137
MalI GATC 2 cut(s) 99, 219
MboI GATC 2 cut(s) 97, 217
MboII GAAGA 4 cut(s) 20, 87, 548, 581
MhlI GDGCHC 5 cut(s) 135, 252, 320, 449, 748
MluCI AATT 6 cut(s) 463, 480, 509, 528, 691, 752
MlyI GAGTC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 5 cut(s) 47, 121, 174, 396, 735
MroXI GAANNNNTTC 2 cut(s) 371, 540
MseI TTAA 4 cut(s) 345, 422, 441, 704
MslI CAYNNNNRTG 2 cut(s) 578, 798
MspA1I CMGCKG 1 cut(s) 825
MspCI CTTAAG 2 cut(s) 344, 440
MspR9I CCNGG 1 cut(s) 295
MvaI CCWGG 1 cut(s) 295
MwoI GCNNNNNNNGC 1 cut(s) 482
NdeII GATC 2 cut(s) 97, 217
NlaIII CATG 5 cut(s) 89, 577, 583, 719, 797
NlaIV GGNNCC 4 cut(s) 132, 133, 134, 782
NspI RCATGY 1 cut(s) 583
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 793
PceI AGGCCT 1 cut(s) 667
PciI ACATGT 1 cut(s) 579
PciSI GCTCTTC 1 cut(s) 70
PdmI GAANNNNTTC 2 cut(s) 371, 540
PfeI GAWTC 2 cut(s) 416, 671
PkrI GCNGC 5 cut(s) 82, 266, 306, 714, 827
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
PscI ACATGT 1 cut(s) 579
Psp6I CCWGG 1 cut(s) 293
PspFI CCCAGC 1 cut(s) 821
PspGI CCWGG 1 cut(s) 293
PspN4I GGNNCC 4 cut(s) 132, 133, 134, 782
PspOMI GGGCCC 1 cut(s) 131
PspPI GGNCC 3 cut(s) 131, 132, 780
PstI CTGCAG 2 cut(s) 65, 309
PvuII CAGCTG 1 cut(s) 825
RsaI GTAC 2 cut(s) 71, 734
RsaNI GTAC 2 cut(s) 70, 733
RseI CAYNNNNRTG 2 cut(s) 578, 798
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 4 cut(s) 345, 422, 441, 704
SatI GCNGC 5 cut(s) 81, 265, 305, 713, 826
Sau3AI GATC 2 cut(s) 97, 217
Sau96I GGNCC 3 cut(s) 131, 132, 780
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 5 cut(s) 135, 252, 320, 449, 748
SetI ASST 6 cut(s) 39, 82, 306, 351, 704, 827
SfcI CTRYAG 4 cut(s) 61, 305, 321, 697
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 1 cut(s) 780
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 2 cut(s) 578, 798
SmlI CTYRAG 3 cut(s) 176, 344, 440
SmoI CTYRAG 3 cut(s) 176, 344, 440
Sse9I AATT 6 cut(s) 463, 480, 509, 528, 691, 752
SseBI AGGCCT 1 cut(s) 667
SsiI CCGC 2 cut(s) 115, 725
SspMI CTAG 1 cut(s) 137
StuI AGGCCT 1 cut(s) 667
StyD4I CCNGG 1 cut(s) 293
StyI CCWWGG 2 cut(s) 136, 277
TaaI ACNGT 2 cut(s) 325, 413
TaqI TCGA 3 cut(s) 119, 177, 562
TasI AATT 6 cut(s) 463, 480, 509, 528, 691, 752
TatI WGTACW 2 cut(s) 69, 732
TfiI GAWTC 2 cut(s) 416, 671
Tru1I TTAA 4 cut(s) 345, 422, 441, 704
Tru9I TTAA 4 cut(s) 345, 422, 441, 704
TscAI CASTG 3 cut(s) 391, 418, 658
TseI GCWGC 5 cut(s) 80, 264, 304, 712, 825
TspDTI ATGAA 4 cut(s) 380, 447, 810, 824
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 3 cut(s) 391, 418, 658
Vha464I CTTAAG 2 cut(s) 344, 440
VneI GTGCAC 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 780
XapI RAATTY 1 cut(s) 509
XceI RCATGY 1 cut(s) 583
XhoI CTCGAG 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 136
XmnI GAANNNNTTC 2 cut(s) 371, 540
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.