MD17G1284100.v1.1

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008305
Physical Location & Seq
Reverse (-)
34307774 .. 34311511
3738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1284100.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGAAGAAGAATCAGAATCAGATGATGATGATGCATGTTTCATTGTTATTGTCACATCTGATTCTTCTGCTGCTCTGTATTATTAGTATCATTGATGGTGCTTTTGCTTCTGGGAAAAGTGATTATGTCACAGCAGTTGGAGACCCAGGAATGAGAAGGGATGAGCTGAGAGTGGCCTTGGAAGCTTGGAACTTCTGCAATGAAGTTGGAGAAGAAGCACCTGATATGGGCAGTCCCAGAGCTGCTGATTGCTTTAATCTCACAAGTACTTCCTTAAAACACGGAGTGAGCAAAAAAGACAACTTGCTTGGGGTTGGGACTCCATTCCCAGGGATAAGCCAGGGGGCTTTAAACAATGTGGACCTTTATGCAGTGGAGAAGGAGCTGTATCTGGGTTCTCTATGTCAAGTCCAAGACTCTCCAAAACCGTGGCAATTCTGGATGATTATGCTCAAAAATGGAAACTTTGACACAATGGCTGGGCTGTGCCCTTCCAATGGGAAAAAGACACCGCCTTTTAGCGATCCCGGAAGGTTCCCTTGCTTTGGGAAGGGCTGTATGAATCAACCCACTGTGGTCCACCAGAGGACTCTGCTGGATGGTAATACCACAATGCGAGGAGGTTTCAATGGGACTTATGATCTGGGATCCAACCCCAGTGATGGGCTCGATTCGATATCTTTCTACGAGGTGGAATGGAAGAAGAAACTTGGTGTTGGAAGCTGGGAGTTCAGGCACATGCTCAAGACTTCAAAGAAGTATCCATGGCTGATGCTCTACCTCAGAGCTGATGCAACCCAAGGATATTCTGGTGGCTATCACTATGATACAAGAGGAATGCTCAAAATTCCTCCCGAGTCACCCAATTTTAAAGTCAGGCTGACTTTGGACATCAAACAAGGAGGAGGACCCAAGAGCCAGTTTTACCTCATTGACATTGGTAGCTGTTGGAAGAACAATGGCAAACCCTGTGACGGCGATGTGCTCACGGATGTGACCAGATACACGGAGATGATCATAAACCCGGAAACTCCTGCTTGGTGCAGCCCCACAGGTTTAGGCAATTGCCCGCCGTACCATATCACTCCAAACGACAGAAAAATTTATAGAAACGACACTGCCAACTTCCCATATGGGGCTTATCACTACTACTGTGCTCCGGGAAATGCTGAACATTTAGAGAAACCTGTAAGCGTATGTGATCCTTACAGTAATCCCCAGGCACAAGAAATAGTTCAGTTGCTCCCTCACCCCATCTGGGCCGAGTACGGGTATCCAACCAAACAAGGGCAGGGTTGGATTGGGGATGCTAGAACTTGGGAGCTTGATGTTGGTGGCCTATCTAGTCGACTCCACTACTACCAGGATCCAGGCACCACTCCTGCTAGAAGGATATGGGGATCAATTGATGTTGGTACAGAAATTTTTGTTAGTGACAAAGATGAAGTGGCAGAGTGGACTCTAAGCGACTTTGACGTTATTTATACACCCCCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

500

Amino Acids

55.66

Weight (kDa)

5.91

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7705 PF24804 44 - 494 3.2e-252 Domain of unknown function (DUF7705)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1373
AccI GTMKAC 1 cut(s) 1348
AciI CCGC 2 cut(s) 512, 1070
AclWI GGATC 7 cut(s) 518, 642, 655, 1196, 1361, 1374, 1408
AcsI RAATTY 3 cut(s) 846, 1101, 1422
AdeI CACNNNGTG 1 cut(s) 286
AfaI GTAC 4 cut(s) 268, 1076, 1268, 1417
AgsI TTSAA 2 cut(s) 628, 753
AjnI CCWGG 6 cut(s) 145, 328, 339, 1218, 1362, 1369
AleI CACNNNNGTG 1 cut(s) 992
AluBI AGCT 8 cut(s) 166, 185, 242, 385, 723, 788, 945, 1324
AluI AGCT 8 cut(s) 166, 185, 242, 385, 723, 788, 945, 1324
Alw21I GWGCWC 2 cut(s) 987, 1159
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 7 cut(s) 518, 642, 655, 1196, 1361, 1374, 1408
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 3 cut(s) 174, 1260, 1336
ApeKI GCWGC 3 cut(s) 70, 242, 1044
ApoI RAATTY 3 cut(s) 846, 1101, 1422
Asp700I GAANNNNTTC 1 cut(s) 1233
AspS9I GGNCC 4 cut(s) 361, 577, 908, 1260
AsuC2I CCSGG 3 cut(s) 528, 1025, 1161
AsuHPI GGTGA 2 cut(s) 852, 1241
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 3 cut(s) 361, 577, 908
BaeGI GKGCMC 1 cut(s) 491
BamHI GGATCC 2 cut(s) 647, 1366
BanI GGYRCC 1 cut(s) 1373
BanII GRGCYC 1 cut(s) 669
Bbv12I GWGCWC 2 cut(s) 987, 1159
BbvI GCAGC 3 cut(s) 57, 229, 1056
BccI CCATC 4 cut(s) 89, 593, 656, 1262
BceAI ACGGC 2 cut(s) 991, 1057
BciT130I CCWGG 6 cut(s) 147, 330, 341, 1220, 1364, 1371
BciVI GTATCC 2 cut(s) 771, 1284
BclI TGATCA 1 cut(s) 1014
BcnI CCSGG 3 cut(s) 528, 1025, 1161
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 3 cut(s) 1311, 1344, 1386
BfuI GTATCC 2 cut(s) 771, 1284
BisI GCNGC 3 cut(s) 71, 243, 1045
BlsI GCNGC 3 cut(s) 72, 244, 1046
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 9 cut(s) 147, 330, 341, 528, 1025, 1161, 1220, 1364, 1371
Bme18I GGWCC 3 cut(s) 361, 577, 908
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 4 cut(s) 361, 577, 908, 1260
BmiI GGNNCC 5 cut(s) 536, 649, 910, 1368, 1375
BmrFI CCNGG 9 cut(s) 147, 330, 341, 528, 1025, 1161, 1220, 1364, 1371
BmrI ACTGGG 1 cut(s) 651
BmsI GCATC 4 cut(s) 21, 762, 781, 1297
BmuI ACTGGG 1 cut(s) 651
BplI GAGNNNNNCTC 2 cut(s) 825, 857
BpuEI CTTGAG 1 cut(s) 728
BpuMI CCSGG 3 cut(s) 528, 1025, 1161
BsaI GGTCTC 1 cut(s) 135
BsaJI CCNNGG 9 cut(s) 145, 177, 328, 329, 340, 428, 764, 799, 1218
Bse1I ACTGG 2 cut(s) 657, 919
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 6 cut(s) 147, 330, 341, 1220, 1364, 1371
BseDI CCNNGG 9 cut(s) 145, 177, 328, 329, 340, 428, 764, 799, 1218
BseGI GGATG 5 cut(s) 166, 447, 604, 997, 1312
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 158, 796
BseNI ACTGG 2 cut(s) 657, 919
BseRI GAGGAG 2 cut(s) 633, 918
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 3 cut(s) 57, 229, 1056
BseYI CCCAGC 2 cut(s) 479, 723
BsgI GTGCAG 1 cut(s) 1063
BshFI GGCC 3 cut(s) 176, 1262, 1338
BshNI GGYRCC 1 cut(s) 1373
BsiHKAI GWGCWC 2 cut(s) 987, 1159
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 3 cut(s) 528, 1025, 1160
BslFI GGGAC 3 cut(s) 219, 331, 646
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 3 cut(s) 219, 331, 646
BsmI GAATGC 1 cut(s) 843
BsnI GGCC 3 cut(s) 176, 1262, 1338
Bso31I GGTCTC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 4 cut(s) 491, 669, 987, 1159
Bsp143I GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
Bsp19I CCATGG 1 cut(s) 764
BspACI CCGC 2 cut(s) 512, 1070
BspANI GGCC 3 cut(s) 176, 1262, 1338
BspCNI CTCAG 2 cut(s) 159, 795
BspLI GGNNCC 5 cut(s) 536, 649, 910, 1368, 1375
BspPI GGATC 7 cut(s) 518, 642, 655, 1196, 1361, 1374, 1408
BspT107I GGYRCC 1 cut(s) 1373
BspTNI GGTCTC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 205
BsrI ACTGG 2 cut(s) 657, 919
BssECI CCNNGG 9 cut(s) 145, 177, 328, 329, 340, 428, 764, 799, 1218
BssMI GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
BssT1I CCWWGG 3 cut(s) 177, 764, 799
Bst2UI CCWGG 6 cut(s) 147, 330, 341, 1220, 1364, 1371
Bst4CI ACNGT 4 cut(s) 429, 574, 1154, 1211
BstC8I GCNNGC 1 cut(s) 1070
BstDEI CTNAG 3 cut(s) 167, 782, 1463
BstDSI CCRYGG 2 cut(s) 428, 764
BstF5I GGATG 5 cut(s) 166, 447, 604, 997, 1312
BstKTI GATC 7 cut(s) 526, 643, 650, 1017, 1204, 1369, 1403
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 6 cut(s) 147, 330, 341, 1220, 1364, 1371
BstNSI RCATGY 2 cut(s) 38, 742
BstSCI CCNGG 9 cut(s) 145, 328, 339, 526, 1023, 1159, 1218, 1362, 1369
BstSLI GKGCMC 1 cut(s) 491
BstV1I GCAGC 3 cut(s) 57, 229, 1056
BstX2I RGATCY 2 cut(s) 647, 1366
BstXI CCANNNNNNTGG 1 cut(s) 429
BstYI RGATCY 2 cut(s) 647, 1366
BsuI GTATCC 2 cut(s) 771, 1284
BsuRI GGCC 3 cut(s) 176, 1262, 1338
BtgI CCRYGG 2 cut(s) 428, 764
BtgZI GCGATG 1 cut(s) 993
BtsCI GGATG 5 cut(s) 166, 447, 604, 997, 1312
BtsI GCAGTG 2 cut(s) 378, 1116
BtsIMutI CAGTG 4 cut(s) 378, 570, 664, 1116
Cac8I GCNNGC 1 cut(s) 1070
Cfr13I GGNCC 4 cut(s) 361, 577, 908, 1260
Csp6I GTAC 4 cut(s) 267, 1075, 1267, 1416
CviAII CATG 3 cut(s) 35, 739, 765
CviQI GTAC 4 cut(s) 267, 1075, 1267, 1416
DdeI CTNAG 3 cut(s) 167, 782, 1463
DpnI GATC 7 cut(s) 525, 642, 649, 1016, 1203, 1368, 1402
DpnII GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
DraI TTTAAA 2 cut(s) 351, 871
DraIII CACNNNGTG 1 cut(s) 286
Eco130I CCWWGG 3 cut(s) 177, 764, 799
Eco24I GRGCYC 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 678
Eco47I GGWCC 3 cut(s) 361, 577, 908
Eco88I CYCGRG 1 cut(s) 854
EcoO109I RGGNCCY 1 cut(s) 908
EcoRII CCWGG 6 cut(s) 145, 328, 339, 1218, 1362, 1369
EcoRV GATATC 1 cut(s) 678
EcoT14I CCWWGG 3 cut(s) 177, 764, 799
EcoT22I ATGCAT 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 3 cut(s) 177, 764, 799
FaeI CATG 3 cut(s) 38, 742, 768
FalI AAGNNNNNCTT 2 cut(s) 523, 555
FaqI GGGAC 3 cut(s) 219, 331, 646
FatI CATG 3 cut(s) 34, 738, 764
FauI CCCGC 1 cut(s) 1077
FauNDI CATATG 1 cut(s) 1132
FbaI TGATCA 1 cut(s) 1014
FblI GTMKAC 1 cut(s) 1348
Fnu4HI GCNGC 3 cut(s) 71, 243, 1045
FokI GGATG 5 cut(s) 173, 454, 611, 1004, 1319
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 3 cut(s) 71, 243, 1045
FspBI CTAG 3 cut(s) 1311, 1344, 1386
GluI GCNGC 3 cut(s) 71, 243, 1045
GsaI CCCAGC 2 cut(s) 483, 727
HaeIII GGCC 3 cut(s) 176, 1262, 1338
HapII CCGG 3 cut(s) 528, 1025, 1160
Hin1II CATG 3 cut(s) 38, 742, 768
HincII GTYRAC 1 cut(s) 1349
HindII GTYRAC 1 cut(s) 1349
HindIII AAGCTT 1 cut(s) 183
HpaII CCGG 3 cut(s) 528, 1025, 1160
HphI GGTGA 2 cut(s) 852, 1241
Hpy166II GTNNAC 4 cut(s) 361, 580, 1349, 1458
Hpy188I TCNGA 4 cut(s) 15, 21, 60, 785
Hpy188III TCNNGA 3 cut(s) 439, 745, 854
Hpy8I GTNNAC 4 cut(s) 361, 580, 1349, 1458
HpyAV CCTTC 6 cut(s) 150, 373, 501, 525, 544, 1383
HpyCH4III ACNGT 4 cut(s) 429, 574, 1154, 1211
HpyCH4IV ACGT 1 cut(s) 1476
HpyCH4V TGCA 5 cut(s) 34, 198, 371, 794, 1044
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 3 cut(s) 167, 782, 1463
HpySE526I ACGT 1 cut(s) 1476
Hsp92II CATG 3 cut(s) 38, 742, 768
Ksp22I TGATCA 1 cut(s) 1014
Kzo9I GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
LmnI GCTCC 4 cut(s) 382, 1162, 1248, 1321
Lsp1109I GCAGC 3 cut(s) 57, 229, 1056
LweI GCATC 4 cut(s) 21, 762, 781, 1297
MaeI CTAG 3 cut(s) 1311, 1344, 1386
MaeII ACGT 1 cut(s) 1476
MaeIII GTNAC 6 cut(s) 51, 127, 858, 971, 994, 1433
MalI GATC 7 cut(s) 525, 642, 649, 1016, 1203, 1368, 1402
MboI GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
MboII GAAGA 7 cut(s) 16, 19, 56, 224, 712, 715, 964
MfeI CAATTG 2 cut(s) 1063, 1404
MflI RGATCY 2 cut(s) 647, 1366
MhlI GDGCHC 4 cut(s) 491, 669, 987, 1159
MluCI AATT 7 cut(s) 434, 846, 865, 1063, 1101, 1404, 1422
MlyI GAGTC 6 cut(s) 313, 410, 583, 866, 1344, 1453
MmeI TCCRAC 7 cut(s) 118, 187, 675, 697, 929, 1277, 1301
Mph1103I ATGCAT 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 1233
MseI TTAA 4 cut(s) 255, 275, 350, 870
MslI CAYNNNNRTG 2 cut(s) 992, 1010
MspI CCGG 3 cut(s) 528, 1025, 1160
MspR9I CCNGG 9 cut(s) 147, 330, 341, 528, 1025, 1161, 1220, 1364, 1371
MunI CAATTG 2 cut(s) 1063, 1404
Mva1269I GAATGC 1 cut(s) 843
MvaI CCWGG 6 cut(s) 147, 330, 341, 1220, 1364, 1371
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 3 cut(s) 528, 1025, 1161
NcoI CCATGG 1 cut(s) 764
NdeI CATATG 1 cut(s) 1132
NdeII GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
NlaIII CATG 3 cut(s) 38, 742, 768
NlaIV GGNNCC 5 cut(s) 536, 649, 910, 1368, 1375
NmeAIII GCCGAG 1 cut(s) 1288
NmuCI GTSAC 6 cut(s) 51, 127, 858, 971, 994, 1433
NsiI ATGCAT 1 cut(s) 36
NspI RCATGY 2 cut(s) 38, 742
OliI CACNNNNGTG 1 cut(s) 992
PasI CCCWGGG 1 cut(s) 329
PctI GAATGC 1 cut(s) 843
PdmI GAANNNNTTC 1 cut(s) 1233
PfeI GAWTC 5 cut(s) 10, 16, 61, 562, 671
PfoI TCCNGGA 2 cut(s) 526, 1159
PkrI GCNGC 3 cut(s) 72, 244, 1046
PleI GAGTC 6 cut(s) 313, 410, 583, 865, 1344, 1453
PpsI GAGTC 6 cut(s) 313, 410, 583, 865, 1344, 1453
PpuMI RGGWCCY 1 cut(s) 908
Psp5II RGGWCCY 1 cut(s) 908
Psp6I CCWGG 6 cut(s) 145, 328, 339, 1218, 1362, 1369
PspFI CCCAGC 2 cut(s) 479, 723
PspGI CCWGG 6 cut(s) 145, 328, 339, 1218, 1362, 1369
PspN4I GGNNCC 5 cut(s) 536, 649, 910, 1368, 1375
PspPI GGNCC 4 cut(s) 361, 577, 908, 1260
PspPPI RGGWCCY 1 cut(s) 908
PsuI RGATCY 2 cut(s) 647, 1366
RsaI GTAC 4 cut(s) 268, 1076, 1268, 1417
RsaNI GTAC 4 cut(s) 267, 1075, 1267, 1416
RseI CAYNNNNRTG 2 cut(s) 992, 1010
SalI GTCGAC 1 cut(s) 1347
SaqAI TTAA 4 cut(s) 255, 275, 350, 870
SatI GCNGC 3 cut(s) 71, 243, 1045
Sau3AI GATC 7 cut(s) 523, 640, 647, 1014, 1201, 1366, 1400
Sau96I GGNCC 4 cut(s) 361, 577, 908, 1260
ScaI AGTACT 1 cut(s) 268
SchI GAGTC 6 cut(s) 313, 410, 583, 866, 1344, 1453
ScrFI CCNGG 9 cut(s) 147, 330, 341, 528, 1025, 1161, 1220, 1364, 1371
SduI GDGCHC 4 cut(s) 491, 669, 987, 1159
SfaNI GCATC 4 cut(s) 21, 762, 781, 1297
SinI GGWCC 3 cut(s) 361, 577, 908
SmiMI CAYNNNNRTG 2 cut(s) 992, 1010
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 7 cut(s) 434, 846, 865, 1063, 1101, 1404, 1422
SsiI CCGC 2 cut(s) 512, 1070
SspMI CTAG 3 cut(s) 1311, 1344, 1386
StyD4I CCNGG 9 cut(s) 145, 328, 339, 526, 1023, 1159, 1218, 1362, 1369
StyI CCWWGG 3 cut(s) 177, 764, 799
TaaI ACNGT 4 cut(s) 429, 574, 1154, 1211
TaiI ACGT 1 cut(s) 1479
TaqI TCGA 3 cut(s) 669, 674, 1348
TasI AATT 7 cut(s) 434, 846, 865, 1063, 1101, 1404, 1422
TatI WGTACW 1 cut(s) 266
TfiI GAWTC 5 cut(s) 10, 16, 61, 562, 671
Tru1I TTAA 4 cut(s) 255, 275, 350, 870
Tru9I TTAA 4 cut(s) 255, 275, 350, 870
TscAI CASTG 4 cut(s) 378, 577, 664, 1123
TseFI GTSAC 6 cut(s) 51, 127, 858, 971, 994, 1433
TseI GCWGC 3 cut(s) 70, 242, 1044
Tsp45I GTSAC 6 cut(s) 51, 127, 858, 971, 994, 1433
TspDTI ATGAA 5 cut(s) 17, 30, 216, 575, 1458
TspGWI ACGGA 3 cut(s) 297, 1004, 1022
TspRI CASTG 4 cut(s) 378, 577, 664, 1123
VpaK11BI GGWCC 3 cut(s) 361, 577, 908
XapI RAATTY 3 cut(s) 846, 1101, 1422
XceI RCATGY 2 cut(s) 38, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 1348
XmnI GAANNNNTTC 1 cut(s) 1233
XspI CTAG 3 cut(s) 1311, 1344, 1386
ZrmI AGTACT 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.