Prupe.1G101500_v2.0.a1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013529
Physical Location & Seq
Reverse (-)
8078426 .. 8087002
8577 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G101500.2

Sequence Viewer

Length: 1536 bp
ATGCATTCAATTTTAACTTTTTTTTTTTTCTTCGTTCTTCTTTTGTCTCTGCAAACAATGCCGGACCTCGGAAACCTCTCTCTCTCAGCTTCTCTAACCCTCACGACCTCCCCTAACTTCAAATTGCTCACTGATTCCCCCGAACAGAGCTTGGTCAAAACCCTAGGCCTTGACTGCGATTGGGACCAGAGGTTGAGGTTCGCGACTCTGCACAATGCCGAGGTCAGCACTGGCGCTGCAATCAGGGTTTTCCGGCGGAGCCGGCCATTGGTGGACAGATCGAAGAAGATCTCCAGCGGCAAGAGTTTGGAGGACAATGTGCGCGAATGGGTTTCCAACAGAGTGGAATTGGGCATTCCTGAGTCGCGGTGCTCGTTGCCGTTTCTTTACGGTGCAAAGAAATTGGTTGAATGTCTTGTTTGCCATTATTATGTCTACCCTGGGGAGGAGGTCGTATGCTCTGTTCGTGGTTGTCAAGGAGTGTATCATGGGAAATGTGTGAAGGAAAGGCTTCGAATCTCTAATATGAAAAAGTTCAAGTGTCAGCAGCATGCATGCTTCATTTGCAGACAGAGGGTAGATTGGCGGTGTATGCGATGCCCTATAGCGTCACATGATAAATGTGCTGCTTGGCCGGATGAAGTGATTTACTTGAAAGACCGGCCAGGGAAGGCTGTTTGTTGGAGGCATCCTACAGATTGGCGGCAAGATAGGAAGCATGTGGTTTCGACAAGCAGCATAGAGGAGGTATTCTGTCGCTTGCCTCTGCCTTACACTGATGAGGAGTTCAAGATTGACTTGACTTGGAAAGATACGGATATTAAATTAGAGCCACCTCCATATGTGCACATCAGGCGTAATATTTACCTAGTCAAGAAGAAGCGTGATGGCAGTGATGATGATGTTGGATGCACATGTCGCACTTCCATATGCTCTGAGGATTGTGTTTGCAGGGGTCAATGCATAAGCTGCTCAAAGGCTTGCCATTGCTCAGAAAATTGCTCAAACCGACCGTTTCGCAAGGAGAAGAAGATCAAAATCGTAAAGACTGAACACTGCGGTTGGGGAGCAGAGGCAGCTGAATCCATTAAGAAAGGCGATTTTATAATTGAGTACATCGGGGAAGTTATTGATGATGCTTTGTGCGAACGAAGGCTTTGGGACATGAAGTACAAAGAGGTGCAGAATTTCTATATGTGTGAAATTCGAAAAGACTTTACAATTGATGCAACTTTCAAAGGAAATCCATCTCGATTTTTGAACCACGGCTGTGATCCCAACTGTGTCCTTGAAAAGTGGCAAGTTGAGGGTGAAACACGGGTGGGTGTGTTTGCTGCAAGATCAATAGAAGTTGGAGAGGCATTAACATACGATTACAGATTCGTGCAATTCGGACCCGAGGTGAAGTGCCATTGTGGTGCTCCAAATTGTCAAGGCTACCTTGGAAGCAAGAGAAAGATTCATAAGGTGGAACTTTGCTGGGGTTCAAAACGCAAAAGAACATCAACTGCTTGCATAGCTATCATTACTCAATGA

Protein Analysis

512

Amino Acids

58.73

Weight (kDa)

8.73

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30860
fragaria_vesca FvH4_4g08820 FvH4_4g08820
malus_domestica MD16G1258900.v1.1
prunus_persica Prupe.1G101500_v2.0.a1 Prupe.1G101500_v2.0.a1 Prupe.1G101500_v2.0.a1
pyrus_communis pycom16g22640
rosa_chinensis RchiOBHm_Chr4g0405361
rosa_laevigata RLG00000008848
rosa_multiflora Rmu_sc0000161.1_g000080
rosa_roxburghii Rroxscaffold_5G00349790
rosa_rugosa Rorug04G0057700
rosa_samantha Rh4AG131200 Rh4BG125800 Rh4CG138100 Rh4DG125200
rosa_wichuraiana Rw4G010630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AccI GTMKAC 1 cut(s) 435
AccII CGCG 3 cut(s) 203, 324, 367
AciI CCGC 6 cut(s) 256, 297, 367, 586, 703, 1059
AclWI GGATC 1 cut(s) 1268
AcoI YGGCCR 3 cut(s) 263, 632, 662
AcsI RAATTY 2 cut(s) 1186, 1203
AfaI GTAC 2 cut(s) 1115, 1172
AfiI CCNNNNNNNGG 3 cut(s) 68, 268, 445
AflIII ACRYGT 1 cut(s) 914
AjnI CCWGG 2 cut(s) 439, 664
AleI CACNNNNGTG 1 cut(s) 1269
AluBI AGCT 5 cut(s) 89, 150, 969, 1079, 1520
AluI AGCT 5 cut(s) 89, 150, 969, 1079, 1520
Alw21I GWGCWC 3 cut(s) 374, 849, 1423
Alw26I GTCTC 1 cut(s) 51
Alw44I GTGCAC 1 cut(s) 845
AlwI GGATC 1 cut(s) 1268
Ama87I CYCGRG 1 cut(s) 1397
AoxI GGCC 4 cut(s) 166, 263, 632, 662
ApaLI GTGCAC 1 cut(s) 845
ApeKI GCWGC 7 cut(s) 236, 547, 626, 735, 969, 1076, 1334
ApoI RAATTY 2 cut(s) 1186, 1203
Asp700I GAANNNNTTC 2 cut(s) 510, 533
AspA2I CCTAGG 1 cut(s) 163
AspLEI GCGC 2 cut(s) 236, 324
AspS9I GGNCC 3 cut(s) 64, 184, 1394
AsuHPI GGTGA 2 cut(s) 1322, 1414
AsuII TTCGAA 2 cut(s) 514, 1207
AvaI CYCGRG 1 cut(s) 1397
AvaII GGWCC 3 cut(s) 64, 184, 1394
AvrII CCTAGG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 849
Bbv12I GWGCWC 3 cut(s) 374, 849, 1423
BbvI GCAGC 7 cut(s) 223, 559, 613, 747, 956, 1088, 1321
BccI CCATC 2 cut(s) 881, 1255
BceAI ACGGC 2 cut(s) 364, 1282
BciT130I CCWGG 2 cut(s) 441, 666
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 2 cut(s) 164, 869
BfmI CTRYAG 2 cut(s) 603, 693
BfoI RGCGCY 1 cut(s) 237
BglII AGATCT 1 cut(s) 288
BisI GCNGC 9 cut(s) 237, 298, 548, 627, 704, 736, 970, 1077, 1335
BlnI CCTAGG 1 cut(s) 163
BlsI GCNGC 9 cut(s) 238, 299, 549, 628, 705, 737, 971, 1078, 1336
Bme1390I CCNGG 2 cut(s) 441, 666
Bme18I GGWCC 3 cut(s) 64, 184, 1394
BmeT110I CYCGRG 1 cut(s) 1397
BmgT120I GGNCC 3 cut(s) 64, 184, 1394
BmiI GGNNCC 3 cut(s) 185, 260, 1396
BmrFI CCNGG 2 cut(s) 441, 666
BmsI GCATC 5 cut(s) 587, 697, 899, 1126, 1216
BpmI CTGGAG 1 cut(s) 277
Bpu14I TTCGAA 2 cut(s) 514, 1207
BsaBI GATNNNNATC 1 cut(s) 1037
BsaJI CCNNGG 9 cut(s) 67, 163, 219, 439, 440, 665, 1264, 1398, 1441
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 268, 445
Bse118I RCCGGY 2 cut(s) 261, 660
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 985
Bse8I GATNNNNATC 1 cut(s) 1037
BseBI CCWGG 2 cut(s) 441, 666
BseDI CCNNGG 9 cut(s) 67, 163, 219, 439, 440, 665, 1264, 1398, 1441
BseGI GGATG 3 cut(s) 643, 688, 914
BseJI GATNNNNATC 1 cut(s) 1037
BseLI CCNNNNNNNGG 3 cut(s) 68, 268, 445
BseMI GCAATG 1 cut(s) 985
BseMII CTCAG 4 cut(s) 99, 351, 927, 1005
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 3 cut(s) 461, 758, 797
BseSI GKGCMC 1 cut(s) 849
BseXI GCAGC 7 cut(s) 223, 559, 613, 747, 956, 1088, 1321
BseYI CCCAGC 1 cut(s) 1479
BsgI GTGCAG 2 cut(s) 194, 1202
Bsh1236I CGCG 3 cut(s) 203, 324, 367
Bsh1285I CGRYCG 1 cut(s) 1013
BshFI GGCC 4 cut(s) 168, 265, 634, 664
BsiEI CGRYCG 1 cut(s) 1013
BsiHKAI GWGCWC 3 cut(s) 374, 849, 1423
BsiHKCI CYCGRG 1 cut(s) 1397
BsiSI CCGG 5 cut(s) 62, 253, 262, 635, 661
BslFI GGGAC 2 cut(s) 197, 1175
BslI CCNNNNNNNGG 3 cut(s) 68, 268, 445
BsmAI GTCTC 1 cut(s) 51
BsmFI GGGAC 2 cut(s) 197, 1175
BsmI GAATGC 2 cut(s) 4, 354
BsnI GGCC 4 cut(s) 168, 265, 634, 664
BsoBI CYCGRG 1 cut(s) 1397
Bsp119I TTCGAA 2 cut(s) 514, 1207
Bsp1286I GDGCHC 3 cut(s) 374, 849, 1423
Bsp143I GATC 5 cut(s) 278, 288, 1032, 1273, 1340
Bsp68I TCGCGA 1 cut(s) 203
BspACI CCGC 6 cut(s) 256, 297, 367, 586, 703, 1059
BspANI GGCC 4 cut(s) 168, 265, 634, 664
BspCNI CTCAG 4 cut(s) 98, 352, 928, 1004
BspFNI CGCG 3 cut(s) 203, 324, 367
BspLI GGNNCC 3 cut(s) 185, 260, 1396
BspPI GGATC 1 cut(s) 1268
BspT104I TTCGAA 2 cut(s) 514, 1207
BsrDI GCAATG 1 cut(s) 985
BsrFI RCCGGY 2 cut(s) 261, 660
BsrI ACTGG 1 cut(s) 235
BssAI RCCGGY 2 cut(s) 261, 660
BssECI CCNNGG 9 cut(s) 67, 163, 219, 439, 440, 665, 1264, 1398, 1441
BssMI GATC 5 cut(s) 278, 288, 1032, 1273, 1340
BssT1I CCWWGG 2 cut(s) 163, 1441
Bst2UI CCWGG 2 cut(s) 441, 666
Bst4CI ACNGT 3 cut(s) 392, 1014, 1283
BstAPI GCANNNNNTGC 2 cut(s) 58, 969
BstBI TTCGAA 2 cut(s) 514, 1207
BstC8I GCNNGC 6 cut(s) 263, 552, 556, 761, 982, 1513
BstDEI CTNAG 4 cut(s) 85, 360, 936, 991
BstDSI CCRYGG 1 cut(s) 1264
BstF5I GGATG 3 cut(s) 643, 688, 914
BstFNI CGCG 3 cut(s) 203, 324, 367
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 324
BstKTI GATC 5 cut(s) 281, 291, 1035, 1276, 1343
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 5 cut(s) 278, 288, 1032, 1273, 1340
BstMCI CGRYCG 1 cut(s) 1013
BstNI CCWGG 2 cut(s) 441, 666
BstNSI RCATGY 4 cut(s) 554, 558, 722, 918
BstSCI CCNGG 2 cut(s) 439, 664
BstSFI CTRYAG 2 cut(s) 603, 693
BstSLI GKGCMC 1 cut(s) 849
BstUI CGCG 3 cut(s) 203, 324, 367
BstV1I GCAGC 7 cut(s) 223, 559, 613, 747, 956, 1088, 1321
BstX2I RGATCY 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 1 cut(s) 288
BsuRI GGCC 4 cut(s) 168, 265, 634, 664
BtgI CCRYGG 1 cut(s) 1264
BtgZI GCGATG 1 cut(s) 610
BtsCI GGATG 3 cut(s) 643, 688, 914
BtsI GCAGTG 2 cut(s) 898, 1054
BtsIMutI CAGTG 5 cut(s) 129, 228, 774, 898, 1054
BtuMI TCGCGA 1 cut(s) 203
Cac8I GCNNGC 6 cut(s) 263, 552, 556, 761, 982, 1513
CfoI GCGC 2 cut(s) 236, 324
Cfr10I RCCGGY 2 cut(s) 261, 660
Cfr13I GGNCC 3 cut(s) 64, 184, 1394
CseI GACGC 1 cut(s) 597
Csp6I GTAC 2 cut(s) 1114, 1171
CviAII CATG 7 cut(s) 488, 551, 555, 614, 719, 915, 1165
CviQI GTAC 2 cut(s) 1114, 1171
DdeI CTNAG 4 cut(s) 85, 360, 936, 991
DpnI GATC 5 cut(s) 280, 290, 1034, 1275, 1342
DpnII GATC 5 cut(s) 278, 288, 1032, 1273, 1340
EaeI YGGCCR 3 cut(s) 263, 632, 662
EciI GGCGGA 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 163, 1441
Eco147I AGGCCT 1 cut(s) 168
Eco47I GGWCC 3 cut(s) 64, 184, 1394
Eco88I CYCGRG 1 cut(s) 1397
EcoRII CCWGG 2 cut(s) 439, 664
EcoT14I CCWWGG 2 cut(s) 163, 1441
EcoT22I ATGCAT 3 cut(s) 6, 556, 965
ErhI CCWWGG 2 cut(s) 163, 1441
FaeI CATG 7 cut(s) 491, 554, 558, 617, 722, 918, 1168
FalI AAGNNNNNCTT 4 cut(s) 782, 814, 1425, 1457
FaqI GGGAC 2 cut(s) 197, 1175
FatI CATG 7 cut(s) 487, 550, 554, 613, 718, 914, 1164
FauNDI CATATG 2 cut(s) 841, 929
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 9 cut(s) 237, 298, 548, 627, 704, 736, 970, 1077, 1335
FokI GGATG 3 cut(s) 650, 675, 921
Fsp4HI GCNGC 9 cut(s) 237, 298, 548, 627, 704, 736, 970, 1077, 1335
FspBI CTAG 2 cut(s) 164, 869
GlaI GCGC 2 cut(s) 235, 323
GluI GCNGC 9 cut(s) 237, 298, 548, 627, 704, 736, 970, 1077, 1335
GsaI CCCAGC 1 cut(s) 1483
GsuI CTGGAG 1 cut(s) 277
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 4 cut(s) 168, 265, 634, 664
HapII CCGG 5 cut(s) 62, 253, 262, 635, 661
HgaI GACGC 1 cut(s) 597
HhaI GCGC 2 cut(s) 236, 324
Hin1II CATG 7 cut(s) 491, 554, 558, 617, 722, 918, 1168
Hin6I GCGC 2 cut(s) 234, 322
HinP1I GCGC 2 cut(s) 234, 322
HinfI GANTC 7 cut(s) 134, 205, 362, 516, 1082, 1380, 1459
HpaII CCGG 5 cut(s) 62, 253, 262, 635, 661
HphI GGTGA 2 cut(s) 1322, 1414
Hpy166II GTNNAC 3 cut(s) 274, 436, 847
Hpy188I TCNGA 4 cut(s) 71, 937, 994, 1394
Hpy188III TCNNGA 6 cut(s) 103, 202, 359, 790, 874, 1251
Hpy8I GTNNAC 3 cut(s) 274, 436, 847
HpyAV CCTTC 3 cut(s) 496, 664, 1146
HpyCH4III ACNGT 3 cut(s) 392, 1014, 1283
HpyF3I CTNAG 4 cut(s) 85, 360, 936, 991
Hsp92II CATG 7 cut(s) 491, 554, 558, 617, 722, 918, 1168
HspAI GCGC 2 cut(s) 234, 322
KroI GCCGGC 1 cut(s) 261
KroNI GCCGGC 1 cut(s) 263
Kzo9I GATC 5 cut(s) 278, 288, 1032, 1273, 1340
LmnI GCTCC 3 cut(s) 258, 1067, 1426
Lsp1109I GCAGC 7 cut(s) 223, 559, 613, 747, 956, 1088, 1321
LweI GCATC 5 cut(s) 587, 697, 899, 1126, 1216
MaeI CTAG 2 cut(s) 164, 869
MaeIII GTNAC 1 cut(s) 609
MalI GATC 5 cut(s) 280, 290, 1034, 1275, 1342
MboI GATC 5 cut(s) 278, 288, 1032, 1273, 1340
MboII GAAGA 7 cut(s) 22, 29, 295, 298, 889, 1039, 1042
MfeI CAATTG 1 cut(s) 1221
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 3 cut(s) 374, 849, 1423
MlyI GAGTC 2 cut(s) 199, 371
MmeI TCCRAC 4 cut(s) 360, 662, 886, 1333
Mph1103I ATGCAT 3 cut(s) 6, 556, 965
MroNI GCCGGC 1 cut(s) 261
MroXI GAANNNNTTC 2 cut(s) 510, 533
MseI TTAA 4 cut(s) 14, 822, 1089, 1364
MslI CAYNNNNRTG 3 cut(s) 429, 1269, 1416
MspA1I CMGCKG 2 cut(s) 297, 1079
MspI CCGG 5 cut(s) 62, 253, 262, 635, 661
MspR9I CCNGG 2 cut(s) 441, 666
MunI CAATTG 1 cut(s) 1221
Mva1269I GAATGC 2 cut(s) 4, 354
MvaI CCWGG 2 cut(s) 441, 666
MvnI CGCG 3 cut(s) 203, 324, 367
NaeI GCCGGC 1 cut(s) 263
NdeI CATATG 2 cut(s) 841, 929
NdeII GATC 5 cut(s) 278, 288, 1032, 1273, 1340
NgoMIV GCCGGC 1 cut(s) 261
NlaIII CATG 7 cut(s) 491, 554, 558, 617, 722, 918, 1168
NlaIV GGNNCC 3 cut(s) 185, 260, 1396
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 609
NruI TCGCGA 1 cut(s) 203
NsiI ATGCAT 3 cut(s) 6, 556, 965
NspI RCATGY 4 cut(s) 554, 558, 722, 918
NspV TTCGAA 2 cut(s) 514, 1207
OliI CACNNNNGTG 1 cut(s) 1269
PaeI GCATGC 2 cut(s) 554, 558
PasI CCCWGGG 1 cut(s) 440
PceI AGGCCT 1 cut(s) 168
PciI ACATGT 1 cut(s) 914
PcsI WCGNNNNNNNCGW 1 cut(s) 371
PctI GAATGC 2 cut(s) 4, 354
PdiI GCCGGC 1 cut(s) 263
PdmI GAANNNNTTC 2 cut(s) 510, 533
PfeI GAWTC 5 cut(s) 134, 516, 1082, 1380, 1459
PkrI GCNGC 9 cut(s) 238, 299, 549, 628, 705, 737, 971, 1078, 1336
PleI GAGTC 2 cut(s) 199, 370
PpsI GAGTC 2 cut(s) 199, 370
PscI ACATGT 1 cut(s) 914
PsiI TTATAA 1 cut(s) 1106
Psp6I CCWGG 2 cut(s) 439, 664
PspFI CCCAGC 1 cut(s) 1479
PspGI CCWGG 2 cut(s) 439, 664
PspN4I GGNNCC 3 cut(s) 185, 260, 1396
PspPI GGNCC 3 cut(s) 64, 184, 1394
PsrI GAACNNNNNNTAC 2 cut(s) 447, 479
PsuI RGATCY 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 1079
RruI TCGCGA 1 cut(s) 203
RsaI GTAC 2 cut(s) 1115, 1172
RsaNI GTAC 2 cut(s) 1114, 1171
RseI CAYNNNNRTG 3 cut(s) 429, 1269, 1416
SaqAI TTAA 4 cut(s) 14, 822, 1089, 1364
SatI GCNGC 9 cut(s) 237, 298, 548, 627, 704, 736, 970, 1077, 1335
Sau3AI GATC 5 cut(s) 278, 288, 1032, 1273, 1340
Sau96I GGNCC 3 cut(s) 64, 184, 1394
SchI GAGTC 2 cut(s) 199, 371
ScrFI CCNGG 2 cut(s) 441, 666
SduI GDGCHC 3 cut(s) 374, 849, 1423
SfaNI GCATC 5 cut(s) 587, 697, 899, 1126, 1216
SfcI CTRYAG 2 cut(s) 603, 693
SfuI TTCGAA 2 cut(s) 514, 1207
SinI GGWCC 3 cut(s) 64, 184, 1394
SmiMI CAYNNNNRTG 3 cut(s) 429, 1269, 1416
SphI GCATGC 2 cut(s) 554, 558
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 6 cut(s) 256, 297, 367, 586, 703, 1059
SspI AATATT 1 cut(s) 862
SspMI CTAG 2 cut(s) 164, 869
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 439, 664
StyI CCWWGG 2 cut(s) 163, 1441
TaaI ACNGT 3 cut(s) 392, 1014, 1283
TaqI TCGA 5 cut(s) 281, 514, 728, 1207, 1252
TatI WGTACW 2 cut(s) 1113, 1170
TauI GCSGC 2 cut(s) 300, 706
TfiI GAWTC 5 cut(s) 134, 516, 1082, 1380, 1459
Tru1I TTAA 4 cut(s) 14, 822, 1089, 1364
Tru9I TTAA 4 cut(s) 14, 822, 1089, 1364
TscAI CASTG 5 cut(s) 136, 235, 781, 898, 1061
TseFI GTSAC 1 cut(s) 609
TseI GCWGC 7 cut(s) 236, 547, 626, 735, 969, 1076, 1334
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 5 cut(s) 542, 550, 654, 1181, 1451
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 5 cut(s) 136, 235, 781, 898, 1061
VneI GTGCAC 1 cut(s) 845
VpaK11BI GGWCC 3 cut(s) 64, 184, 1394
XapI RAATTY 2 cut(s) 1186, 1203
XceI RCATGY 4 cut(s) 554, 558, 722, 918
XmaJI CCTAGG 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 435
XmnI GAANNNNTTC 2 cut(s) 510, 533
XspI CTAG 2 cut(s) 164, 869
Zsp2I ATGCAT 3 cut(s) 6, 556, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.