Rorug04G0057700

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013529
Physical Location & Seq
Reverse (-)
9037057 .. 9037545
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0057700.1

Sequence Viewer

Length: 489 bp
ATGGTATTAGAGTTGTTTTACCTAGGAAAAAACATTGTTAACAGGTTAGACAACGTATTGAACTACCATTGTCACTCCGGAGATGATGATCTTGGTTATTGTAGCCTACCAGCCAATCAGGAATGGGAGTTTGGGTTTCGTGCAAAGTTTACGACAAGATTCGACTGTCATGTATCGTACTCGAATTACGATCTCAATGTCACGGTATTTGAAATGAGTGCTGCCTATGAAAATGCTTGTGGCGGAGCTCATTGCATATGGAAATTTTTACAAGATGGAATTTACTTGTACAACATCCCCTTTGGTGAAGATGTAAGAAAGTACACTTGGCATGCAACATCAATAATTATGTTCGTTGTTTCTCTTTTGTTTGCTCCGTTATTGTATCCGTTGCTCTTTGTTTTAGTGCTTCCTTTGAGGCCTAGTTCATTGTATGTTGTTTCTTTTCCATGTCTATGCTCTGTCAAATTTGCTTGTAATGTTGTTTAA

Protein Analysis

162

Amino Acids

18.58

Weight (kDa)

5.63

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 12 - 110 1.8e-19 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013529)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30860
fragaria_vesca FvH4_4g08820 FvH4_4g08820
malus_domestica MD16G1258900.v1.1
prunus_persica Prupe.1G101500_v2.0.a1 Prupe.1G101500_v2.0.a1 Prupe.1G101500_v2.0.a1
pyrus_communis pycom16g22640
rosa_chinensis RchiOBHm_Chr4g0405361
rosa_laevigata RLG00000008848
rosa_multiflora Rmu_sc0000161.1_g000080
rosa_roxburghii Rroxscaffold_5G00349790
rosa_rugosa Rorug04G0057700
rosa_samantha Rh4AG131200 Rh4BG125800 Rh4CG138100 Rh4DG125200
rosa_wichuraiana Rw4G010630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 1 cut(s) 243
AcsI RAATTY 3 cut(s) 263, 279, 467
AfaI GTAC 3 cut(s) 179, 290, 323
AgsI TTSAA 2 cut(s) 61, 212
AjuI GAANNNNNNNTTGG 4 cut(s) 114, 146, 310, 342
AluBI AGCT 1 cut(s) 248
AluI AGCT 1 cut(s) 248
Alw21I GWGCWC 1 cut(s) 250
Aor13HI TCCGGA 1 cut(s) 77
AoxI GGCC 1 cut(s) 419
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 3 cut(s) 263, 279, 467
AspA2I CCTAGG 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 317
AvrII CCTAGG 1 cut(s) 22
BanII GRGCYC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 208
BccI CCATC 1 cut(s) 269
BciVI GTATCC 1 cut(s) 396
BfaI CTAG 2 cut(s) 23, 423
BfuI GTATCC 1 cut(s) 396
BisI GCNGC 1 cut(s) 222
BlnI CCTAGG 1 cut(s) 22
BlsI GCNGC 1 cut(s) 223
BsaBI GATNNNNATC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 22
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 87
BseAI TCCGGA 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 1 cut(s) 294
BseJI GATNNNNATC 1 cut(s) 87
BseMI GCAATG 1 cut(s) 250
BseXI GCAGC 1 cut(s) 208
BshFI GGCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 1 cut(s) 78
BsnI GGCC 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 250
Bsp13I TCCGGA 1 cut(s) 77
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 2 cut(s) 88, 190
BspACI CCGC 1 cut(s) 243
BspANI GGCC 1 cut(s) 421
BspEI TCCGGA 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 250
BsrGI TGTACA 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 2 cut(s) 88, 190
BssT1I CCWWGG 1 cut(s) 22
Bst4CI ACNGT 2 cut(s) 167, 205
BstAUI TGTACA 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 333
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 2 cut(s) 91, 193
BstMBI GATC 2 cut(s) 88, 190
BstNSI RCATGY 1 cut(s) 335
BstV1I GCAGC 1 cut(s) 208
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 1 cut(s) 421
BtsCI GGATG 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 333
Csp6I GTAC 3 cut(s) 178, 289, 322
CviAII CATG 3 cut(s) 170, 332, 450
CviJI RGCY 4 cut(s) 105, 113, 248, 421
CviKI_1 RGCY 4 cut(s) 105, 113, 248, 421
CviQI GTAC 3 cut(s) 178, 289, 322
DpnI GATC 2 cut(s) 90, 192
DpnII GATC 2 cut(s) 88, 190
EciI GGCGGA 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 22
Eco147I AGGCCT 1 cut(s) 421
Eco24I GRGCYC 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 248
EcoT14I CCWWGG 1 cut(s) 22
EcoT38I GRGCYC 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 3 cut(s) 173, 335, 453
FaiI YATR 9 cut(s) 171, 228, 257, 259, 333, 350, 435, 451, 457
FatI CATG 3 cut(s) 169, 331, 449
FauNDI CATATG 1 cut(s) 257
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 1 cut(s) 281
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 2 cut(s) 23, 423
GluI GCNGC 1 cut(s) 222
HaeIII GGCC 1 cut(s) 421
HapII CCGG 1 cut(s) 78
Hin1II CATG 3 cut(s) 173, 335, 453
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 1 cut(s) 159
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 1 cut(s) 78
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 3 cut(s) 40, 150, 324
Hpy188III TCNNGA 2 cut(s) 78, 119
Hpy8I GTNNAC 3 cut(s) 40, 150, 324
HpyCH4III ACNGT 2 cut(s) 167, 205
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 3 cut(s) 143, 255, 335
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 3 cut(s) 173, 335, 453
Kpn2I TCCGGA 1 cut(s) 77
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 88, 190
LmnI GCTCC 2 cut(s) 245, 379
LpnPI CCDG 4 cut(s) 28, 91, 104, 123
Lsp1109I GCAGC 1 cut(s) 208
MaeI CTAG 2 cut(s) 23, 423
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 71, 199
MalI GATC 2 cut(s) 90, 192
MboI GATC 2 cut(s) 88, 190
MboII GAAGA 1 cut(s) 320
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 5 cut(s) 184, 263, 279, 345, 467
MnlI CCTC 1 cut(s) 411
MroI TCCGGA 1 cut(s) 77
MseI TTAA 2 cut(s) 39, 487
MslI CAYNNNNRTG 1 cut(s) 454
MspI CCGG 1 cut(s) 78
NdeI CATATG 1 cut(s) 257
NdeII GATC 2 cut(s) 88, 190
NlaIII CATG 3 cut(s) 173, 335, 453
NmuCI GTSAC 2 cut(s) 71, 199
NspI RCATGY 1 cut(s) 335
PaeI GCATGC 1 cut(s) 335
PceI AGGCCT 1 cut(s) 421
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 1 cut(s) 223
Psp124BI GAGCTC 1 cut(s) 250
RsaI GTAC 3 cut(s) 179, 290, 323
RsaNI GTAC 3 cut(s) 178, 289, 322
RseI CAYNNNNRTG 1 cut(s) 454
SacI GAGCTC 1 cut(s) 250
SaqAI TTAA 2 cut(s) 39, 487
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 2 cut(s) 88, 190
SduI GDGCHC 1 cut(s) 250
SetI ASST 4 cut(s) 24, 47, 57, 250
SmiMI CAYNNNNRTG 1 cut(s) 454
SphI GCATGC 1 cut(s) 335
Sse9I AATT 5 cut(s) 184, 263, 279, 345, 467
SseBI AGGCCT 1 cut(s) 421
SsiI CCGC 1 cut(s) 243
SspMI CTAG 2 cut(s) 23, 423
SstI GAGCTC 1 cut(s) 250
StuI AGGCCT 1 cut(s) 421
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 167, 205
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 162, 182
TasI AATT 5 cut(s) 184, 263, 279, 345, 467
TatI WGTACW 2 cut(s) 288, 321
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 2 cut(s) 39, 487
Tru9I TTAA 2 cut(s) 39, 487
TseFI GTSAC 2 cut(s) 71, 199
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 2 cut(s) 71, 199
TspDTI ATGAA 2 cut(s) 243, 417
TspGWI ACGGA 2 cut(s) 366, 378
XapI RAATTY 3 cut(s) 263, 279, 467
XceI RCATGY 1 cut(s) 335
XmaJI CCTAGG 1 cut(s) 22
XspI CTAG 2 cut(s) 23, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.