Prupe.1G379200_v2.0.a1

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016040
Physical Location & Seq
Reverse (-)
34250715 .. 34253603
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G379200.1

Sequence Viewer

Length: 1104 bp
ATGCCATATGTTCTTCATTCCTTTCTATTGGTTTCTCAGTTTCCCTGCACTCTAAAGACCTTTCCTTGCAATCTAAAGACCATGTCAAGTCAAGAAAATGGCTCACCTGAACACCCAACATGGCCGGAGTTCAAGCTTCCTGACTTGTTGTCTACGGACACTGTTCGACAAGTCCATGCAACAATTGAGAATGAATGGGATCCCCTTCAGCGGTCTGCATGCCAAACTGCAGCTGGTAGAGCCTTGTGGAAGCATGTGATTCATGACCCCCTGGCAGACATACTTGCTGGAGAGACGTATCTAAAAAATCTTCACGAGAAGATAAAGAAAGACTGTGTCAACAAAGCACGAGAAATCTCTGGAGTCATTCTTGCAGTTCGAACCCTCTGGTTTGATTCAAAACTTGAAGCAGCACTTCATTCCTTCAATGACAGAGAAGCACAAGTAGTTCTTCTTGGGGCAGGAATGGATGCAAGGGCTTACCGATTGAGTTGCTTGAAGGAAAGCAATGTGTTCGAAGTTGATTTTCCTGAAGTCCTGCAAATTAAAGCCACTCTTCTGCAAGCAGCGATGGATTCTACAAACAATCACCAGTATCTAACGATGACAGCAAAATCCATAACCAGAGTGGCGGCTGATATCAGAGGCAATGACTGGCTCGAAAAACTTCAAATATCAGGGTTTGTGCCAGAGAGAAACACAGTGTGGGTTCTGGAAGGCATCCTCTACTACTTATCCCACTCTGAGGCCACACAAGTACTTGAAATCATAGCAGACAAGTGCTCGCTTACTAACACAGTGCTCTTAGCAGATTTTATGAATAAACCATCAACCAGTCTCTCCAGTTCCATCTTCCATTTCTACAGCGATTGGCCTGATCATCTCCTGCCATCTCTTGGCTTTTCTTACGTTAAACTTTCACAAATTGGTGATCCAGATGCTCATTTTGGGCTCATGCACGATCCCTTAAATCTGTTCAACAAGCTCCGCAGCTTGCCTAGGTCAGTATATACCCACCCAGATGATGGAACACCATGTTGTCGCTTGTATTTGGTTGAGGCTTCTGGTTCACCAAAGCAAACTATTCCATCGATGGCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LCM

Protein Analysis

368

Amino Acids

41.21

Weight (kDa)

5.95

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42760 AT5G42760
fragaria_vesca FvH4_2g39790
malus_domestica MD15G1027400.v1.1
prunus_persica Prupe.1G379200_v2.0.a1
pyrus_communis pycom15g02340
rosa_laevigata RLG00000011280
rosa_multiflora Rmu_sc0003168.1_g000012 Rmu_sc0035551.1_g000003
rosa_roxburghii Rroxscaffold_7G00167210
rosa_rugosa Rorug06G0300600
rosa_samantha Rh6AG414400 Rh6BG421300 Rh6CG428900 Rh6DG414300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 896, 1025
AccI GTMKAC 1 cut(s) 152
AciI CCGC 3 cut(s) 211, 632, 988
AclWI GGATC 4 cut(s) 194, 207, 926, 956
AcoI YGGCCR 2 cut(s) 122, 1095
AcuI CTGAAG 2 cut(s) 191, 552
AdeI CACNNNGTG 1 cut(s) 705
AfaI GTAC 1 cut(s) 759
AfiI CCNNNNNNNGG 4 cut(s) 210, 745, 896, 1025
AgsI TTSAA 8 cut(s) 133, 399, 407, 427, 499, 671, 764, 979
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 5 cut(s) 136, 233, 985, 993, 1101
AluI AGCT 5 cut(s) 136, 233, 985, 993, 1101
Alw21I GWGCWC 2 cut(s) 785, 804
Alw26I GTCTC 2 cut(s) 287, 842
AlwI GGATC 4 cut(s) 194, 207, 926, 956
AoxI GGCC 4 cut(s) 122, 747, 872, 1095
ApeKI GCWGC 4 cut(s) 230, 410, 566, 990
Asp700I GAANNNNTTC 1 cut(s) 666
AspA2I CCTAGG 1 cut(s) 998
AsuHPI GGTGA 4 cut(s) 96, 581, 941, 1062
AsuII TTCGAA 2 cut(s) 379, 516
AvrII CCTAGG 1 cut(s) 998
BalI TGGCCA 1 cut(s) 1097
BamHI GGATCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 954
BauI CACGAG 2 cut(s) 314, 348
Bbv12I GWGCWC 2 cut(s) 785, 804
BbvI GCAGC 4 cut(s) 242, 422, 578, 1002
BccI CCATC 7 cut(s) 565, 835, 857, 898, 1019, 1087, 1096
BcgI CGANNNNNNTGC 4 cut(s) 474, 496, 508, 530
BciT130I CCWGG 1 cut(s) 272
BclI TGATCA 1 cut(s) 877
BcoDI GTCTC 2 cut(s) 287, 842
BfaI CTAG 1 cut(s) 999
BfmI CTRYAG 2 cut(s) 228, 862
BisI GCNGC 5 cut(s) 231, 411, 567, 633, 991
BlnI CCTAGG 1 cut(s) 998
BlsI GCNGC 5 cut(s) 232, 412, 568, 634, 992
BmcAI AGTACT 1 cut(s) 759
Bme1390I CCNGG 1 cut(s) 272
BmeRI GACNNNNNGTC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 3 cut(s) 460, 729, 928
BpmI CTGGAG 3 cut(s) 309, 381, 826
Bpu14I TTCGAA 2 cut(s) 379, 516
Bsa29I ATCGAT 1 cut(s) 1091
BsaJI CCNNGG 2 cut(s) 270, 998
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 745, 896, 1025
Bse1I ACTGG 4 cut(s) 592, 659, 834, 843
Bse3DI GCAATG 2 cut(s) 514, 655
BseBI CCWGG 1 cut(s) 272
BseCI ATCGAT 1 cut(s) 1091
BseDI CCNNGG 2 cut(s) 270, 998
BseGI GGATG 2 cut(s) 475, 720
BseLI CCNNNNNNNGG 4 cut(s) 210, 745, 896, 1025
BseMI GCAATG 2 cut(s) 514, 655
BseMII CTCAG 2 cut(s) 50, 735
BseNI ACTGG 4 cut(s) 592, 659, 834, 843
BseXI GCAGC 4 cut(s) 242, 422, 578, 1002
BsgI GTGCAG 1 cut(s) 31
BshFI GGCC 4 cut(s) 124, 749, 874, 1097
BshVI ATCGAT 1 cut(s) 1091
BsiHKAI GWGCWC 2 cut(s) 785, 804
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 4 cut(s) 210, 745, 896, 1025
BsmAI GTCTC 2 cut(s) 287, 842
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 4 cut(s) 124, 749, 874, 1097
Bsp119I TTCGAA 2 cut(s) 379, 516
Bsp1286I GDGCHC 3 cut(s) 785, 804, 954
Bsp143I GATC 4 cut(s) 199, 877, 931, 961
BspACI CCGC 3 cut(s) 211, 632, 988
BspANI GGCC 4 cut(s) 124, 749, 874, 1097
BspCNI CTCAG 2 cut(s) 49, 736
BspDI ATCGAT 1 cut(s) 1091
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 201
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 4 cut(s) 194, 207, 926, 956
BspT104I TTCGAA 2 cut(s) 379, 516
BsrDI GCAATG 2 cut(s) 514, 655
BsrI ACTGG 4 cut(s) 592, 659, 834, 843
BssECI CCNNGG 2 cut(s) 270, 998
BssMI GATC 4 cut(s) 199, 877, 931, 961
BssSI CACGAG 2 cut(s) 314, 348
BssT1I CCWWGG 1 cut(s) 998
Bst2BI CACGAG 2 cut(s) 314, 348
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 163, 335, 703, 799
Bst6I CTCTTC 1 cut(s) 561
BstBI TTCGAA 2 cut(s) 379, 516
BstC8I GCNNGC 5 cut(s) 220, 564, 785, 995, 1099
BstDEI CTNAG 3 cut(s) 36, 744, 805
BstF5I GGATG 2 cut(s) 475, 720
BstKTI GATC 4 cut(s) 202, 880, 934, 964
BstMAI GTCTC 2 cut(s) 287, 842
BstMBI GATC 4 cut(s) 199, 877, 931, 961
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 2 cut(s) 222, 257
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 2 cut(s) 228, 862
BstV1I GCAGC 4 cut(s) 242, 422, 578, 1002
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 1091
BsuRI GGCC 4 cut(s) 124, 749, 874, 1097
BsuTUI ATCGAT 1 cut(s) 1091
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 2 cut(s) 475, 720
BtsIMutI CAGTG 3 cut(s) 159, 708, 804
Cac8I GCNNGC 5 cut(s) 220, 564, 785, 995, 1099
CciI TCATGA 1 cut(s) 262
ClaI ATCGAT 1 cut(s) 1091
Csp6I GTAC 1 cut(s) 758
CviAII CATG 8 cut(s) 82, 120, 176, 219, 254, 263, 955, 1035
CviQI GTAC 1 cut(s) 758
DdeI CTNAG 3 cut(s) 36, 744, 805
DpnI GATC 4 cut(s) 201, 879, 933, 963
DpnII GATC 4 cut(s) 199, 877, 931, 961
DraIII CACNNNGTG 1 cut(s) 705
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 122, 1095
Eam1104I CTCTTC 1 cut(s) 561
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 561
Eco130I CCWWGG 1 cut(s) 998
Eco24I GRGCYC 1 cut(s) 954
Eco32I GATATC 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 191, 552
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 998
EcoT38I GRGCYC 1 cut(s) 954
ErhI CCWWGG 1 cut(s) 998
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 8 cut(s) 85, 123, 179, 222, 257, 266, 958, 1038
FalI AAGNNNNNCTT 6 cut(s) 399, 431, 435, 467, 540, 572
FatI CATG 8 cut(s) 81, 119, 175, 218, 253, 262, 954, 1034
FauNDI CATATG 1 cut(s) 7
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 231, 411, 567, 633, 991
FokI GGATG 2 cut(s) 482, 707
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 5 cut(s) 231, 411, 567, 633, 991
FspBI CTAG 1 cut(s) 999
GluI GCNGC 5 cut(s) 231, 411, 567, 633, 991
GsuI CTGGAG 3 cut(s) 309, 381, 826
HaeIII GGCC 4 cut(s) 124, 749, 874, 1097
HapII CCGG 1 cut(s) 125
Hin1II CATG 8 cut(s) 85, 123, 179, 222, 257, 266, 958, 1038
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 4 cut(s) 259, 363, 395, 575
HpaII CCGG 1 cut(s) 125
HphI GGTGA 4 cut(s) 96, 581, 941, 1062
Hpy166II GTNNAC 3 cut(s) 153, 340, 1070
Hpy188I TCNGA 2 cut(s) 644, 745
Hpy188III TCNNGA 8 cut(s) 92, 140, 263, 314, 360, 530, 713, 935
Hpy8I GTNNAC 3 cut(s) 153, 340, 1070
HpyAV CCTTC 4 cut(s) 215, 433, 493, 710
HpyCH4III ACNGT 4 cut(s) 163, 335, 703, 799
HpyCH4IV ACGT 2 cut(s) 296, 909
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 3 cut(s) 36, 744, 805
HpySE526I ACGT 2 cut(s) 296, 909
Hsp92II CATG 8 cut(s) 85, 123, 179, 222, 257, 266, 958, 1038
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 4 cut(s) 199, 877, 931, 961
LmnI GCTCC 1 cut(s) 990
Lsp1109I GCAGC 4 cut(s) 242, 422, 578, 1002
LweI GCATC 3 cut(s) 460, 729, 928
MaeI CTAG 1 cut(s) 999
MaeII ACGT 2 cut(s) 296, 909
MalI GATC 4 cut(s) 201, 879, 933, 963
MboI GATC 4 cut(s) 199, 877, 931, 961
MboII GAAGA 6 cut(s) 5, 302, 331, 443, 548, 844
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 3 cut(s) 785, 804, 954
MlsI TGGCCA 1 cut(s) 1097
MluCI AATT 3 cut(s) 183, 543, 924
MluNI TGGCCA 1 cut(s) 1097
MlyI GAGTC 1 cut(s) 372
MnlI CCTC 5 cut(s) 395, 638, 734, 739, 1051
Mox20I TGGCCA 1 cut(s) 1097
MroXI GAANNNNTTC 1 cut(s) 666
MscI TGGCCA 1 cut(s) 1097
MseI TTAA 3 cut(s) 546, 912, 968
MslI CAYNNNNRTG 1 cut(s) 1020
Msp20I TGGCCA 1 cut(s) 1097
MspA1I CMGCKG 3 cut(s) 211, 233, 1101
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 239
NdeI CATATG 1 cut(s) 7
NdeII GATC 4 cut(s) 199, 877, 931, 961
NlaIII CATG 8 cut(s) 85, 123, 179, 222, 257, 266, 958, 1038
NlaIV GGNNCC 1 cut(s) 201
NspI RCATGY 2 cut(s) 222, 257
NspV TTCGAA 2 cut(s) 379, 516
PaeI GCATGC 1 cut(s) 222
PagI TCATGA 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 3 cut(s) 259, 395, 575
PflFI GACNNNGTC 2 cut(s) 82, 335
PflMI CCANNNNNTGG 2 cut(s) 896, 1025
PkrI GCNGC 5 cut(s) 232, 412, 568, 634, 992
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 201
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 199
PsyI GACNNNGTC 2 cut(s) 82, 335
PvuII CAGCTG 2 cut(s) 233, 1101
RsaI GTAC 1 cut(s) 759
RsaNI GTAC 1 cut(s) 758
RseI CAYNNNNRTG 1 cut(s) 1020
SaqAI TTAA 3 cut(s) 546, 912, 968
SatI GCNGC 5 cut(s) 231, 411, 567, 633, 991
Sau3AI GATC 4 cut(s) 199, 877, 931, 961
ScaI AGTACT 1 cut(s) 759
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 3 cut(s) 785, 804, 954
SfaNI GCATC 3 cut(s) 460, 729, 928
SfcI CTRYAG 2 cut(s) 228, 862
SfuI TTCGAA 2 cut(s) 379, 516
SmiMI CAYNNNNRTG 1 cut(s) 1020
SphI GCATGC 1 cut(s) 222
Sse9I AATT 3 cut(s) 183, 543, 924
SsiI CCGC 3 cut(s) 211, 632, 988
SspMI CTAG 1 cut(s) 999
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 998
TaaI ACNGT 4 cut(s) 163, 335, 703, 799
TaiI ACGT 2 cut(s) 299, 912
TaqI TCGA 5 cut(s) 166, 379, 516, 660, 1091
TasI AATT 3 cut(s) 183, 543, 924
TatI WGTACW 1 cut(s) 757
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 3 cut(s) 259, 395, 575
Tru1I TTAA 3 cut(s) 546, 912, 968
Tru9I TTAA 3 cut(s) 546, 912, 968
TscAI CASTG 3 cut(s) 166, 708, 804
TseI GCWGC 4 cut(s) 230, 410, 566, 990
TspDTI ATGAA 5 cut(s) 5, 207, 251, 407, 833
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 3 cut(s) 166, 708, 804
Tth111I GACNNNGTC 2 cut(s) 82, 335
Van91I CCANNNNNTGG 2 cut(s) 896, 1025
XceI RCATGY 2 cut(s) 222, 257
XcmI CCANNNNNNNNNTGG 3 cut(s) 230, 625, 1022
XmaJI CCTAGG 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 666
XspI CTAG 1 cut(s) 999
ZrmI AGTACT 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.