Rh6AG414400

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0016040
Physical Location & Seq
Forward (+)
60972214 .. 60975807
3594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG414400.1

Sequence Viewer

Length: 1092 bp
ATGCCATATCTTCTTCATTCGTTTCTAGTCATTTCTAATTTTCCGGGTAAACTGAAGGTGTTTCCTTGCAATCTGAAGACCATGTCAAGTCGAGAAAATGGCTCATCTGAATTCCCAACTTGGCCGGAGTACAAACTTCCGGATTTGTTATCTACTGAAAGCGTTCGACAAGTCCATGCAGCGATTGAAAGCGAATGGGATCCTCTTCAACGGTCTGCGTGCCAAACAGCAGCTGGAAGAGCCTTGTGGAAGCATGTGGTCCATGACCCGCTGGCAGACATACTTGCAGGAGAGACGTACCTAAAATCTCTCCATGAGAAGATAAAGAAAGACTGCGTCAACAAGGCACGAGAAATCTCTGGGGTGATTCTTGCAGTTCGAACACTGTGGTTTGATTCAAAACTTGAAGCAGCACTCCATTCCTTCGACAACAGAGAAGCACAAGTAGTTCTCCTTGGTGCAGGAATGGATGCAAGGGCTTACCGATTGAGTTGCTTGAAGGAAAGTAATGTGTTTGAAGTAGATTTTCCTGACGTCTTGCAAATCAAAGCTACTCTTCTGCAAGCAGCAATGGATTCTACAAATGATCACCAGTATCTAATGATGACAGCAAAATCCATAACCAGAGTTGAGGCTGACATCAGAAGCAATGACTGGCTTGAAAAACTTCAAATATCAGGATTTATGCCAGAGAAGAACACAGTATGGGTTCTGGAAGGTATCTTGTACTACCTAACCCATCCTCAGGCCACGGAAGTATTGAAAATCATAGCAGACAAGTGCTCTCTTACGCAAACAGTACTCTTGGCAGATTTCATGAACAAACCATCAACCAGTCTTTCCAGCTCCAACTTTCATTTCTACAGTGATTGGCCCGATCATCTCCTGCCATCTCTCGGCTTTTCTCATGTTAAACTTTCACAAATTGGTGATCCAGATGCTCATTTTGGGCTCCTGCACGATCCCTTAAATCTGTTCAACAAACTCCGCAGCTTGCCTAGATCAGTACAAACAAACCCAGATGATGGAACACCATGTTGTCGCTTGTATTTGGTTGAGGCTTCTGGTTCACCAAAGCAAAATATTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LCM

Protein Analysis

363

Amino Acids

40.81

Weight (kDa)

6.11

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 73 - 257 6.8e-41 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42760 AT5G42760
fragaria_vesca FvH4_2g39790
malus_domestica MD15G1027400.v1.1
prunus_persica Prupe.1G379200_v2.0.a1
pyrus_communis pycom15g02340
rosa_laevigata RLG00000011280
rosa_multiflora Rmu_sc0003168.1_g000012 Rmu_sc0035551.1_g000003
rosa_roxburghii Rroxscaffold_7G00167210
rosa_rugosa Rorug06G0300600
rosa_samantha Rh6AG414400 Rh6BG421300 Rh6CG428900 Rh6DG414300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 537
AccB7I CCANNNNNTGG 1 cut(s) 1025
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 2 cut(s) 269, 988
AclWI GGATC 4 cut(s) 194, 207, 926, 956
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 2 cut(s) 74, 95
AcyI GRCGYC 1 cut(s) 534
AfaI GTAC 5 cut(s) 131, 299, 728, 801, 1008
AfiI CCNNNNNNNGG 3 cut(s) 745, 896, 1025
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 4 cut(s) 233, 551, 846, 993
AluI AGCT 4 cut(s) 233, 551, 846, 993
Alw21I GWGCWC 1 cut(s) 785
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 4 cut(s) 194, 207, 926, 956
AlwNI CAGNNNCTG 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 3 cut(s) 122, 747, 872
ApeKI GCWGC 5 cut(s) 179, 230, 410, 566, 990
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 2 cut(s) 162, 666
AspS9I GGNCC 2 cut(s) 259, 873
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 4 cut(s) 376, 581, 941, 1062
AsuII TTCGAA 1 cut(s) 379
AvaII GGWCC 1 cut(s) 259
AxyI CCTNAGG 1 cut(s) 744
BamHI GGATCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 954
BauI CACGAG 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 785
BbvI GCAGC 5 cut(s) 191, 242, 422, 578, 1002
BccI CCATC 4 cut(s) 747, 835, 898, 1019
BcgI CGANNNNNNTGC 2 cut(s) 474, 508
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 2 cut(s) 26, 999
BfmI CTRYAG 1 cut(s) 862
BisI GCNGC 5 cut(s) 180, 231, 411, 567, 991
BlsI GCNGC 5 cut(s) 181, 232, 412, 568, 992
BmcAI AGTACT 1 cut(s) 801
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 2 cut(s) 259, 873
BmiI GGNNCC 2 cut(s) 201, 953
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 2 cut(s) 460, 928
BpiI GAAGAC 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 379
BpuMI CCSGG 1 cut(s) 45
BsaHI GRCGYC 1 cut(s) 534
BsaJI CCNNGG 2 cut(s) 454, 750
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 745, 896, 1025
Bse1I ACTGG 3 cut(s) 592, 659, 834
Bse21I CCTNAGG 1 cut(s) 744
Bse3DI GCAATG 2 cut(s) 576, 655
BseAI TCCGGA 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 454, 750
BseGI GGATG 2 cut(s) 475, 739
BseLI CCNNNNNNNGG 3 cut(s) 745, 896, 1025
BseMI GCAATG 2 cut(s) 576, 655
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 3 cut(s) 592, 659, 834
BseXI GCAGC 5 cut(s) 191, 242, 422, 578, 1002
BsgI GTGCAG 2 cut(s) 480, 941
BshFI GGCC 3 cut(s) 124, 749, 874
BsiHKAI GWGCWC 1 cut(s) 785
BsiSI CCGG 3 cut(s) 44, 125, 140
BslI CCNNNNNNNGG 3 cut(s) 745, 896, 1025
BsmAI GTCTC 1 cut(s) 287
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 3 cut(s) 124, 749, 874
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 785, 954
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
BspACI CCGC 2 cut(s) 269, 988
BspANI GGCC 3 cut(s) 124, 749, 874
BspCNI CTCAG 1 cut(s) 757
BspEI TCCGGA 1 cut(s) 139
BspHI TCATGA 1 cut(s) 816
BspLI GGNNCC 2 cut(s) 201, 953
BspPI GGATC 4 cut(s) 194, 207, 926, 956
BspQI GCTCTTC 1 cut(s) 232
BspT104I TTCGAA 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 576, 655
BsrI ACTGG 3 cut(s) 592, 659, 834
BssECI CCNNGG 2 cut(s) 454, 750
BssMI GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
BssNI GRCGYC 1 cut(s) 534
BssSI CACGAG 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 454
Bst2BI CACGAG 1 cut(s) 348
Bst4CI ACNGT 5 cut(s) 213, 387, 703, 799, 866
Bst6I CTCTTC 3 cut(s) 210, 232, 561
BstACI GRCGYC 1 cut(s) 534
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 4 cut(s) 220, 273, 564, 995
BstDEI CTNAG 1 cut(s) 744
BstDSI CCRYGG 1 cut(s) 750
BstF5I GGATG 2 cut(s) 475, 739
BstKTI GATC 6 cut(s) 202, 589, 880, 934, 964, 1004
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 862
BstV1I GCAGC 5 cut(s) 191, 242, 422, 578, 1002
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
Bsu36I CCTNAGG 1 cut(s) 744
BsuRI GGCC 3 cut(s) 124, 749, 874
BtgI CCRYGG 1 cut(s) 750
BtsCI GGATG 2 cut(s) 475, 739
BtsIMutI CAGTG 2 cut(s) 383, 871
Cac8I GCNNGC 4 cut(s) 220, 273, 564, 995
CaiI CAGNNNCTG 1 cut(s) 233
CciI TCATGA 1 cut(s) 816
Cfr13I GGNCC 2 cut(s) 259, 873
CseI GACGC 1 cut(s) 325
Csp6I GTAC 5 cut(s) 130, 298, 727, 800, 1007
CviAII CATG 8 cut(s) 82, 176, 254, 263, 314, 817, 908, 1035
CviQI GTAC 5 cut(s) 130, 298, 727, 800, 1007
DdeI CTNAG 1 cut(s) 744
DpnI GATC 6 cut(s) 201, 588, 879, 933, 963, 1003
DpnII GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 3 cut(s) 210, 232, 561
EarI CTCTTC 3 cut(s) 210, 232, 561
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 954
Eco47I GGWCC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 74, 95
Eco81I CCTNAGG 1 cut(s) 744
EcoRI GAATTC 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 954
ErhI CCWWGG 1 cut(s) 454
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 8 cut(s) 85, 179, 257, 266, 317, 820, 911, 1038
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FatI CATG 8 cut(s) 81, 175, 253, 262, 313, 816, 907, 1034
FauI CCCGC 1 cut(s) 276
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 5 cut(s) 180, 231, 411, 567, 991
FokI GGATG 2 cut(s) 482, 726
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 5 cut(s) 180, 231, 411, 567, 991
FspBI CTAG 2 cut(s) 26, 999
GluI GCNGC 5 cut(s) 180, 231, 411, 567, 991
HaeIII GGCC 3 cut(s) 124, 749, 874
HapII CCGG 3 cut(s) 44, 125, 140
HgaI GACGC 1 cut(s) 325
Hin1I GRCGYC 1 cut(s) 534
Hin1II CATG 8 cut(s) 85, 179, 257, 266, 317, 820, 911, 1038
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 3 cut(s) 367, 395, 575
HpaII CCGG 3 cut(s) 44, 125, 140
HphI GGTGA 4 cut(s) 376, 581, 941, 1062
Hpy166II GTNNAC 3 cut(s) 50, 340, 1070
Hpy188I TCNGA 3 cut(s) 75, 109, 644
Hpy188III TCNNGA 7 cut(s) 92, 140, 530, 678, 713, 817, 935
Hpy8I GTNNAC 3 cut(s) 50, 340, 1070
HpyAV CCTTC 4 cut(s) 49, 433, 493, 710
HpyCH4III ACNGT 5 cut(s) 213, 387, 703, 799, 866
HpyCH4IV ACGT 2 cut(s) 296, 534
HpyCH4V TGCA 9 cut(s) 69, 179, 287, 374, 461, 473, 541, 562, 958
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 1 cut(s) 744
HpySE526I ACGT 2 cut(s) 296, 534
Hsp92I GRCGYC 1 cut(s) 534
Hsp92II CATG 8 cut(s) 85, 179, 257, 266, 317, 820, 911, 1038
Kpn2I TCCGGA 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 2 cut(s) 851, 957
Lsp1109I GCAGC 5 cut(s) 191, 242, 422, 578, 1002
LweI GCATC 2 cut(s) 460, 928
MaeI CTAG 2 cut(s) 26, 999
MaeII ACGT 2 cut(s) 296, 534
MalI GATC 6 cut(s) 201, 588, 879, 933, 963, 1003
MboI GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
MboII GAAGA 7 cut(s) 5, 88, 197, 249, 331, 548, 706
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 2 cut(s) 785, 954
MluCI AATT 3 cut(s) 37, 110, 924
MmeI TCCRAC 1 cut(s) 873
MnlI CCTC 4 cut(s) 213, 625, 753, 1051
MroI TCCGGA 1 cut(s) 139
MroXI GAANNNNTTC 2 cut(s) 162, 666
MseI TTAA 2 cut(s) 912, 968
MspA1I CMGCKG 2 cut(s) 233, 271
MspI CCGG 3 cut(s) 44, 125, 140
MspR9I CCNGG 1 cut(s) 45
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 45
NdeII GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
NlaIII CATG 8 cut(s) 85, 179, 257, 266, 317, 820, 911, 1038
NlaIV GGNNCC 2 cut(s) 201, 953
NmeAIII GCCGAG 1 cut(s) 876
NspI RCATGY 1 cut(s) 257
NspV TTCGAA 1 cut(s) 379
PagI TCATGA 1 cut(s) 816
PciSI GCTCTTC 1 cut(s) 232
PdmI GAANNNNTTC 2 cut(s) 162, 666
PfeI GAWTC 3 cut(s) 367, 395, 575
PflFI GACNNNGTC 2 cut(s) 82, 335
PflMI CCANNNNNTGG 1 cut(s) 1025
PkrI GCNGC 5 cut(s) 181, 232, 412, 568, 992
PspN4I GGNNCC 2 cut(s) 201, 953
PspPI GGNCC 2 cut(s) 259, 873
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 199
PsyI GACNNNGTC 2 cut(s) 82, 335
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 5 cut(s) 131, 299, 728, 801, 1008
RsaNI GTAC 5 cut(s) 130, 298, 727, 800, 1007
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 2 cut(s) 912, 968
SatI GCNGC 5 cut(s) 180, 231, 411, 567, 991
Sau3AI GATC 6 cut(s) 199, 586, 877, 931, 961, 1001
Sau96I GGNCC 2 cut(s) 259, 873
ScaI AGTACT 1 cut(s) 801
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 2 cut(s) 785, 954
SfaNI GCATC 2 cut(s) 460, 928
SfcI CTRYAG 1 cut(s) 862
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 1 cut(s) 259
Sse9I AATT 3 cut(s) 37, 110, 924
SsiI CCGC 2 cut(s) 269, 988
SspI AATATT 1 cut(s) 1084
SspMI CTAG 2 cut(s) 26, 999
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 5 cut(s) 213, 387, 703, 799, 866
TaiI ACGT 2 cut(s) 299, 537
TaqI TCGA 4 cut(s) 91, 166, 379, 426
TasI AATT 3 cut(s) 37, 110, 924
TatI WGTACW 4 cut(s) 129, 726, 799, 1006
TfiI GAWTC 3 cut(s) 367, 395, 575
Tru1I TTAA 2 cut(s) 912, 968
Tru9I TTAA 2 cut(s) 912, 968
TscAI CASTG 2 cut(s) 390, 871
TseI GCWGC 5 cut(s) 179, 230, 410, 566, 990
TspDTI ATGAA 4 cut(s) 5, 805, 833, 845
TspGWI ACGGA 1 cut(s) 767
TspRI CASTG 2 cut(s) 390, 871
Tth111I GACNNNGTC 2 cut(s) 82, 335
Van91I CCANNNNNTGG 1 cut(s) 1025
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 110
XceI RCATGY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XmnI GAANNNNTTC 2 cut(s) 162, 666
XspI CTAG 2 cut(s) 26, 999
ZraI GACGTC 1 cut(s) 535
ZrmI AGTACT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.