Prupe.1G411300_v2.0.a1

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015555
Physical Location & Seq
Reverse (-)
35913394 .. 35915526
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G411300.1

Sequence Viewer

Length: 1983 bp
ATGTCGCCGAAGCAGAAGAATTTTCATCTTCTCTCTGCCTTCTTTTTCTCACTCTTCCAATTCTTCTTCTTTCTTTCTCTCTCTAACCCAGAAGAAACCACCGCCTTGCTAGCATTCAAAGCCTCCACCGACCCCTTCAATTCGCTCTCCTCCTGGATGGCCTCACCCGACCCGTGCTCCGGCTCCTGGCTCGGCGTCACCTGCGACCCAGAGACCCGCCGGGTCACCCGACTCGTGCTCGAGAACCTCAACCTGACCGGGGCGGCTGAGCAACTCAGCCAGCTCGACCAGCTGAAAGTCCTGAGCCTCAAACACAACCTGCTCTCCACCTCGCTCGACTTCTGGTCTTTGCCCCACCTCAACCACCTCTACCTCTCCCACAACCGCTTCGCCGGAACCTTCCCCGCCGGAATCTCCCGCCTCCGCAATCTCCGTCGACTCGACCTCTCGTACAATCAGTTCTCCGGCGACATTCCGTTGACCAAGTTGACTCAGCTGCCTCGCCTGCTAACTCTCCGTCTCGAGTCCAACTCGTTCACCGGGACTCTCGGCTCAGCGGACTTGGATACCGGTACGATTTCGGACTTCAATGTTTCGCACAACAACCTCTCGGGCGAAATCCCTGCGTGGCTGTCTAAATTCGCGGCGACGTCGTTTGCCGGAAACGGGAATCTCTGCGGCAAACCCTTGCCGTACGAATGCTCCAATGGGACGGTCCCTGACTCGTCCGTACCATCCAGGGACTTGGGTCGGAAAAACGGCAGCGTTTTGAGTAACGCTGCACAGCTCACGATCATTATCGTCGGCATAGCTGTTGCGTTGTTAGTGATAGTCGTGACCGCCACGTGGTGTCGGCGCACTAAGAGAGTGGAGGGCGGGACCCGCAACAAGACAGCCAACGGATTCGGTTACGTGAGCAAGCACTACGGGCCCGGAGAAATGAAGAAGAAGAAGAGCAAAGGTGGAGCCCATTTGTCCCAGTCTAACGGGCACGGGCCTGGTGGTGGGCCCAGAGAAAGCGAAGATATGGTGGTGCTGGAAGGGTGCAGGGGCGTCGATAAGGTGGGTGATCTGTTGAAGGCGTCGGCGGAGATGTTGGGGAAGGGCAGCGTGGGGACCACTTTCAAGGTTGCGATGGACGGTGGAGATGTAGTTGTGGTGAAGAGATTGAGGGAGAAGAGAAATGGCGGGAAAGAAATGGATGGATTTTTGAGACAGATTGGTGGGCTGAGGCACTGTAACATTGTCAGCCTCCGAGCGTACCACAATTCCAACTATGAGTTGCTTTTGGTCTACGATTTCATGCCCAAAGGAAGCTTGCATTTTGTGTTGCATGGAAACAGAGGACCTGGAAGAACACCATTGGATTGGGCTACAAGGTTGAAATTAGCTTCTGGGTCTGCGCAAGGACTGGCTTTCCTCCATGGTTGCAACAAGGCCAAGCTCTTTCATGGAAACCTCACTTCATCAAACATCTTGGTGGATCAGATGGGCAGCGCTTGCATCGCAGACATTGGCCTCCAGCAGCTCTTGCCTGCTCCATTTTCATCAAACAGCGCTTACAAAGCCCCTGAATTGATGCTTCCCAGCAGTAAAATCCCAAGAAAGTTTACACAAAAGTGTGATGTTTATAGCTTTGGGGTGGTTCTGATGGAGATTTTGACTGGAAAAACGGCTGGTGAAGAAGGAGAGATGAGCTTGGTGGAGTGGGTTAAGATGGTGGTGCAGAAAGAGTGCAGTACATGGGAAGTGCTGGATTTTGAGCTTCTGGGAGACAAAGAAATGGAGGAGGAAATGTGGGCTCTTCTGCAGGTGGGTTTGCTGTGTGTGTCTCCATTGCCTAAAGATCGTCCAACAATGGGCATGGTGCATCGGATGATTGAGGACATTAGGCAAAAGGGTGTTAGAGAAGATGGGGCAAAGTCTATTTTAGATGACCTTACTTCTGATTCTTCACCTTCCCATTCAGAAAGCACTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

661

Amino Acids

72.03

Weight (kDa)

8.67

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015555)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31790
malus_domestica MD15G1058800.v1.1
prunus_persica Prupe.1G411300_v2.0.a1
pyrus_communis pycom08g05190 pycom15g05520
rosa_chinensis RchiOBHm_Chr6g0311031
rosa_laevigata RLG00000010441
rosa_multiflora Rmu_sc0012235.1_g000006 Rmu_sc0014619.1_g000002
rosa_roxburghii Rroxscaffold_7G00158090
rosa_rugosa Rorug06G0384900
rosa_samantha Rh6AG498700 Rh6BG509300 Rh6CG515100 Rh6DG501700
rosa_wichuraiana Rw6G043430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 209, 1801
AatII GACGTC 1 cut(s) 653
Acc16I TGCGCA 1 cut(s) 1404
Acc36I ACCTGC 3 cut(s) 209, 327, 1801
AccI GTMKAC 2 cut(s) 436, 1293
AccII CGCG 1 cut(s) 644
AclWI GGATC 1 cut(s) 1491
AcsI RAATTY 2 cut(s) 19, 638
AcvI CACGTG 1 cut(s) 846
AcyI GRCGYC 4 cut(s) 195, 650, 1053, 1082
AdeI CACNNNGTG 1 cut(s) 849
AfaI GTAC 6 cut(s) 452, 574, 695, 732, 1262, 1741
AfeI AGCGCT 2 cut(s) 1498, 1558
AfiI CCNNNNNNNGG 7 cut(s) 179, 186, 259, 666, 846, 1004, 1859
AgeI ACCGGT 1 cut(s) 569
AgsI TTSAA 6 cut(s) 118, 139, 589, 1078, 1126, 1384
AhdI GACNNNNNGTC 1 cut(s) 343
AjnI CCWGG 5 cut(s) 152, 185, 737, 997, 1348
AleI CACNNNNGTG 1 cut(s) 1618
Alw21I GWGCWC 2 cut(s) 179, 240
Alw26I GTCTC 5 cut(s) 206, 524, 1207, 1767, 1836
AlwI GGATC 1 cut(s) 1491
Ama87I CYCGRG 3 cut(s) 239, 521, 610
Aor51HI AGCGCT 2 cut(s) 1498, 1558
AoxI GGCC 6 cut(s) 159, 929, 995, 1007, 1437, 1516
ApaI GGGCCC 2 cut(s) 933, 1011
ApeKI GCWGC 6 cut(s) 496, 762, 779, 1107, 1494, 1525
ApoI RAATTY 2 cut(s) 19, 638
AsiGI ACCGGT 1 cut(s) 569
AspLEI GCGC 4 cut(s) 858, 1405, 1499, 1559
AspS9I GGNCC 9 cut(s) 715, 879, 929, 930, 995, 1007, 1008, 1116, 1346
AsuC2I CCSGG 4 cut(s) 221, 259, 541, 933
AsuHPI GGTGA 8 cut(s) 156, 190, 217, 529, 1079, 1171, 1691, 1947
AsuNHI GCTAGC 1 cut(s) 109
AvaI CYCGRG 3 cut(s) 239, 521, 610
AvaII GGWCC 4 cut(s) 715, 879, 1116, 1346
BaeGI GKGCMC 3 cut(s) 933, 993, 1011
BanII GRGCYC 4 cut(s) 933, 970, 1011, 1804
BauI CACGAG 1 cut(s) 233
BbrPI CACGTG 1 cut(s) 846
Bbv12I GWGCWC 2 cut(s) 179, 240
BbvCI CCTCAGC 1 cut(s) 1229
BbvI GCAGC 6 cut(s) 483, 766, 774, 1119, 1506, 1537
BccI CCATC 8 cut(s) 151, 742, 1129, 1196, 1483, 1645, 1711, 1907
BceAI ACGGC 3 cut(s) 676, 775, 1689
BcgI CGANNNNNNTGC 4 cut(s) 605, 639, 1036, 1070
BciT130I CCWGG 5 cut(s) 154, 187, 739, 999, 1350
BciVI GTATCC 1 cut(s) 559
BcnI CCSGG 4 cut(s) 221, 259, 541, 933
BcoDI GTCTC 5 cut(s) 206, 524, 1207, 1767, 1836
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 1808
BfoI RGCGCY 2 cut(s) 1500, 1560
BfuAI ACCTGC 3 cut(s) 209, 327, 1801
BfuI GTATCC 1 cut(s) 559
BisI GCNGC 9 cut(s) 264, 497, 645, 679, 763, 780, 1108, 1495, 1526
BlpI GCTNAGC 2 cut(s) 267, 553
BlsI GCNGC 9 cut(s) 265, 498, 646, 680, 764, 781, 1109, 1496, 1527
Bme1390I CCNGG 9 cut(s) 154, 187, 221, 259, 541, 739, 933, 999, 1350
Bme18I GGWCC 4 cut(s) 715, 879, 1116, 1346
BmeRI GACNNNNNGTC 1 cut(s) 343
BmeT110I CYCGRG 3 cut(s) 239, 521, 610
BmgT120I GGNCC 9 cut(s) 715, 879, 929, 930, 995, 1007, 1008, 1116, 1346
BmiI GGNNCC 9 cut(s) 184, 397, 717, 880, 881, 931, 967, 1009, 1117
BmrFI CCNGG 9 cut(s) 154, 187, 221, 259, 541, 739, 933, 999, 1350
BmrI ACTGGG 1 cut(s) 973
BmsI GCATC 3 cut(s) 1512, 1569, 1879
BmtI GCTAGC 1 cut(s) 113
BmuI ACTGGG 1 cut(s) 973
BoxI GACNNNNGTC 1 cut(s) 747
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 1 cut(s) 1505
Bpu10I CCTNAGC 2 cut(s) 302, 1229
Bpu1102I GCTNAGC 2 cut(s) 267, 553
BpuMI CCSGG 4 cut(s) 221, 259, 541, 933
BsaAI YACGTR 2 cut(s) 846, 913
BsaBI GATNNNNATC 1 cut(s) 797
BsaHI GRCGYC 4 cut(s) 195, 650, 1053, 1082
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 3 cut(s) 258, 738, 1423
BsaWI WCCGGW 1 cut(s) 569
Bsc4I CCNNNNNNNGG 7 cut(s) 179, 186, 259, 666, 846, 1004, 1859
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 3 cut(s) 979, 1416, 1669
Bse3DI GCAATG 1 cut(s) 1835
Bse8I GATNNNNATC 1 cut(s) 797
BseBI CCWGG 5 cut(s) 154, 187, 739, 999, 1350
BseDI CCNNGG 3 cut(s) 258, 738, 1423
BseGI GGATG 4 cut(s) 162, 734, 1207, 1881
BseJI GATNNNNATC 1 cut(s) 797
BseLI CCNNNNNNNGG 7 cut(s) 179, 186, 259, 666, 846, 1004, 1859
BseMI GCAATG 1 cut(s) 1835
BseMII CTCAG 6 cut(s) 258, 289, 293, 506, 567, 1220
BseNI ACTGG 3 cut(s) 979, 1416, 1669
BseRI GAGGAG 2 cut(s) 139, 1802
BseSI GKGCMC 3 cut(s) 933, 993, 1011
BseXI GCAGC 6 cut(s) 483, 766, 774, 1119, 1506, 1537
BseYI CCCAGC 1 cut(s) 1586
BsgI GTGCAG 4 cut(s) 765, 1066, 1745, 1756
Bsh1236I CGCG 1 cut(s) 644
BshFI GGCC 6 cut(s) 161, 931, 997, 1009, 1439, 1518
BshTI ACCGGT 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 179, 240
BsiHKCI CYCGRG 3 cut(s) 239, 521, 610
BsiWI CGTACG 1 cut(s) 693
BslFI GGGAC 7 cut(s) 556, 701, 724, 755, 892, 961, 1129
BslI CCNNNNNNNGG 7 cut(s) 179, 186, 259, 666, 846, 1004, 1859
BsmAI GTCTC 5 cut(s) 206, 524, 1207, 1767, 1836
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 7 cut(s) 556, 701, 724, 755, 892, 961, 1129
BsmI GAATGC 2 cut(s) 113, 704
BsnI GGCC 6 cut(s) 161, 931, 997, 1009, 1439, 1518
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 3 cut(s) 239, 521, 610
Bsp120I GGGCCC 2 cut(s) 929, 1007
Bsp1286I GDGCHC 7 cut(s) 179, 240, 933, 970, 993, 1011, 1804
Bsp143I GATC 4 cut(s) 792, 1069, 1483, 1846
Bsp1720I GCTNAGC 2 cut(s) 267, 553
Bsp19I CCATGG 1 cut(s) 1423
BspANI GGCC 6 cut(s) 161, 931, 997, 1009, 1439, 1518
BspCNI CTCAG 6 cut(s) 259, 288, 294, 505, 566, 1221
BspFNI CGCG 1 cut(s) 644
BspLI GGNNCC 9 cut(s) 184, 397, 717, 880, 881, 931, 967, 1009, 1117
BspMAI CTGCAG 1 cut(s) 1812
BspMI ACCTGC 3 cut(s) 209, 327, 1801
BspOI GCTAGC 1 cut(s) 113
BspPI GGATC 1 cut(s) 1491
BspQI GCTCTTC 2 cut(s) 947, 1809
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 1835
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 3 cut(s) 979, 1416, 1669
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 3 cut(s) 258, 738, 1423
BssMI GATC 4 cut(s) 792, 1069, 1483, 1846
BssNI GRCGYC 4 cut(s) 195, 650, 1053, 1082
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 1423
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 5 cut(s) 154, 187, 739, 999, 1350
Bst4CI ACNGT 3 cut(s) 715, 1142, 1238
Bst6I CTCTTC 5 cut(s) 59, 947, 1157, 1172, 1809
BstACI GRCGYC 4 cut(s) 195, 650, 1053, 1082
BstAPI GCANNNNNTGC 2 cut(s) 1500, 1531
BstBAI YACGTR 2 cut(s) 846, 913
BstC8I GCNNGC 7 cut(s) 111, 281, 506, 920, 1319, 1501, 1536
BstDEI CTNAG 7 cut(s) 267, 275, 302, 492, 553, 861, 1229
BstDSI CCRYGG 1 cut(s) 1423
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 4 cut(s) 162, 734, 1207, 1881
BstFNI CGCG 1 cut(s) 644
BstH2I RGCGCY 2 cut(s) 1500, 1560
BstHHI GCGC 4 cut(s) 858, 1405, 1499, 1559
BstKTI GATC 4 cut(s) 795, 1072, 1486, 1849
BstMAI GTCTC 5 cut(s) 206, 524, 1207, 1767, 1836
BstMBI GATC 4 cut(s) 792, 1069, 1483, 1846
BstNI CCWGG 5 cut(s) 154, 187, 739, 999, 1350
BstPAI GACNNNNGTC 1 cut(s) 747
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 9 cut(s) 152, 185, 219, 257, 539, 737, 931, 997, 1348
BstSFI CTRYAG 1 cut(s) 1808
BstSLI GKGCMC 3 cut(s) 933, 993, 1011
BstUI CGCG 1 cut(s) 644
BstV1I GCAGC 6 cut(s) 483, 766, 774, 1119, 1506, 1537
BstXI CCANNNNNNTGG 2 cut(s) 745, 1368
BsuI GTATCC 1 cut(s) 559
BsuRI GGCC 6 cut(s) 161, 931, 997, 1009, 1439, 1518
BtgI CCRYGG 1 cut(s) 1423
BtgZI GCGATG 2 cut(s) 1148, 1489
BtsCI GGATG 4 cut(s) 162, 734, 1207, 1881
BtsIMutI CAGTG 1 cut(s) 1234
BveI ACCTGC 3 cut(s) 209, 327, 1801
Cac8I GCNNGC 7 cut(s) 111, 281, 506, 920, 1319, 1501, 1536
CfoI GCGC 4 cut(s) 858, 1405, 1499, 1559
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 9 cut(s) 715, 879, 929, 930, 995, 1007, 1008, 1116, 1346
CseI GACGC 3 cut(s) 184, 1042, 1071
Csp6I GTAC 6 cut(s) 451, 573, 694, 731, 1261, 1740
CspAI ACCGGT 1 cut(s) 569
CviAII CATG 6 cut(s) 1303, 1334, 1424, 1451, 1743, 1864
CviQI GTAC 6 cut(s) 451, 573, 694, 731, 1261, 1740
DdeI CTNAG 7 cut(s) 267, 275, 302, 492, 553, 861, 1229
DpnI GATC 4 cut(s) 794, 1071, 1485, 1848
DpnII GATC 4 cut(s) 792, 1069, 1483, 1846
DraIII CACNNNGTG 1 cut(s) 849
DriI GACNNNNNGTC 1 cut(s) 343
Eam1104I CTCTTC 5 cut(s) 59, 947, 1157, 1172, 1809
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 5 cut(s) 59, 947, 1157, 1172, 1809
EciI GGCGGA 1 cut(s) 1103
Eco130I CCWWGG 1 cut(s) 1423
Eco24I GRGCYC 4 cut(s) 933, 970, 1011, 1804
Eco31I GGTCTC 1 cut(s) 206
Eco47I GGWCC 4 cut(s) 715, 879, 1116, 1346
Eco47III AGCGCT 2 cut(s) 1498, 1558
Eco72I CACGTG 1 cut(s) 846
Eco88I CYCGRG 3 cut(s) 239, 521, 610
Eco91I GGTNACC 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 879, 1346
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 5 cut(s) 152, 185, 737, 997, 1348
EcoT14I CCWWGG 1 cut(s) 1423
EcoT38I GRGCYC 4 cut(s) 933, 970, 1011, 1804
ErhI CCWWGG 1 cut(s) 1423
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 6 cut(s) 1306, 1337, 1427, 1454, 1746, 1867
FaqI GGGAC 7 cut(s) 556, 701, 724, 755, 892, 961, 1129
FatI CATG 6 cut(s) 1302, 1333, 1423, 1450, 1742, 1863
FauI CCCGC 6 cut(s) 224, 412, 425, 869, 890, 1181
FblI GTMKAC 2 cut(s) 436, 1293
Fnu4HI GCNGC 9 cut(s) 264, 497, 645, 679, 763, 780, 1108, 1495, 1526
FokI GGATG 4 cut(s) 169, 721, 1214, 1888
FriOI GRGCYC 4 cut(s) 933, 970, 1011, 1804
Fsp4HI GCNGC 9 cut(s) 264, 497, 645, 679, 763, 780, 1108, 1495, 1526
FspBI CTAG 1 cut(s) 110
FspI TGCGCA 1 cut(s) 1404
GlaI GCGC 4 cut(s) 857, 1404, 1498, 1558
GluI GCNGC 9 cut(s) 264, 497, 645, 679, 763, 780, 1108, 1495, 1526
GsaI CCCAGC 1 cut(s) 1590
GsuI CTGGAG 1 cut(s) 1505
HaeII RGCGCY 2 cut(s) 1500, 1560
HaeIII GGCC 6 cut(s) 161, 931, 997, 1009, 1439, 1518
HgaI GACGC 3 cut(s) 184, 1042, 1071
HhaI GCGC 4 cut(s) 858, 1405, 1499, 1559
Hin1I GRCGYC 4 cut(s) 195, 650, 1053, 1082
Hin1II CATG 6 cut(s) 1306, 1337, 1427, 1454, 1746, 1867
Hin6I GCGC 4 cut(s) 856, 1403, 1497, 1557
HinP1I GCGC 4 cut(s) 856, 1403, 1497, 1557
HincII GTYRAC 3 cut(s) 437, 480, 489
HindII GTYRAC 3 cut(s) 437, 480, 489
HindIII AAGCTT 1 cut(s) 1315
HphI GGTGA 8 cut(s) 156, 190, 217, 529, 1079, 1171, 1691, 1947
Hpy166II GTNNAC 6 cut(s) 437, 480, 489, 537, 1294, 1611
Hpy188I TCNGA 8 cut(s) 583, 753, 1256, 1488, 1650, 1875, 1948, 1969
Hpy188III TCNNGA 5 cut(s) 241, 301, 521, 790, 835
Hpy8I GTNNAC 6 cut(s) 437, 480, 489, 537, 1294, 1611
Hpy99I CGWCG 6 cut(s) 438, 652, 655, 806, 1058, 1087
HpyAV CCTTC 8 cut(s) 49, 145, 409, 1034, 1072, 1096, 1679, 1968
HpyCH4III ACNGT 3 cut(s) 715, 1142, 1238
HpyCH4IV ACGT 3 cut(s) 650, 845, 912
HpyF3I CTNAG 7 cut(s) 267, 275, 302, 492, 553, 861, 1229
HpySE526I ACGT 3 cut(s) 650, 845, 912
Hsp92I GRCGYC 4 cut(s) 195, 650, 1053, 1082
Hsp92II CATG 6 cut(s) 1306, 1337, 1427, 1454, 1746, 1867
HspAI GCGC 4 cut(s) 856, 1403, 1497, 1557
KflI GGGWCCC 1 cut(s) 879
Kzo9I GATC 4 cut(s) 792, 1069, 1483, 1846
LguI GCTCTTC 2 cut(s) 947, 1809
LmnI GCTCC 5 cut(s) 182, 188, 707, 965, 1543
Lsp1109I GCAGC 6 cut(s) 483, 766, 774, 1119, 1506, 1537
LweI GCATC 3 cut(s) 1512, 1569, 1879
MaeI CTAG 1 cut(s) 110
MaeII ACGT 3 cut(s) 650, 845, 912
MaeIII GTNAC 6 cut(s) 196, 223, 773, 835, 908, 1238
MalI GATC 4 cut(s) 794, 1071, 1485, 1848
MboI GATC 4 cut(s) 792, 1069, 1483, 1846
MhlI GDGCHC 7 cut(s) 179, 240, 933, 970, 993, 1011, 1804
MluCI AATT 7 cut(s) 19, 59, 139, 638, 1267, 1385, 1574
MlyI GAGTC 6 cut(s) 225, 432, 484, 533, 538, 716
MmeI TCCRAC 4 cut(s) 552, 731, 1296, 1877
MseI TTAA 1 cut(s) 1713
MslI CAYNNNNRTG 2 cut(s) 1478, 1618
MspA1I CMGCKG 3 cut(s) 292, 496, 557
MspR9I CCNGG 9 cut(s) 154, 187, 221, 259, 541, 739, 933, 999, 1350
Mva1269I GAATGC 2 cut(s) 113, 704
MvaI CCWGG 5 cut(s) 154, 187, 739, 999, 1350
MvnI CGCG 1 cut(s) 644
NciI CCSGG 4 cut(s) 221, 259, 541, 933
NcoI CCATGG 1 cut(s) 1423
NdeII GATC 4 cut(s) 792, 1069, 1483, 1846
NheI GCTAGC 1 cut(s) 109
NlaIII CATG 6 cut(s) 1306, 1337, 1427, 1454, 1746, 1867
NlaIV GGNNCC 9 cut(s) 184, 397, 717, 880, 881, 931, 967, 1009, 1117
NmeAIII GCCGAG 2 cut(s) 171, 528
NmuCI GTSAC 3 cut(s) 196, 223, 835
NsbI TGCGCA 1 cut(s) 1404
OliI CACNNNNGTG 1 cut(s) 1618
PaeR7I CTCGAG 2 cut(s) 239, 521
PaqCI CACCTGC 2 cut(s) 209, 1801
PciSI GCTCTTC 2 cut(s) 947, 1809
PctI GAATGC 2 cut(s) 113, 704
PfeI GAWTC 4 cut(s) 411, 670, 903, 1949
Pfl23II CGTACG 1 cut(s) 693
PfoI TCCNGGA 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 569
PkrI GCNGC 9 cut(s) 265, 498, 646, 680, 764, 781, 1109, 1496, 1527
PleI GAGTC 6 cut(s) 225, 432, 484, 532, 538, 716
PmaCI CACGTG 1 cut(s) 846
PmlI CACGTG 1 cut(s) 846
PpsI GAGTC 6 cut(s) 225, 432, 484, 532, 538, 716
Ppu21I YACGTR 2 cut(s) 846, 913
PpuMI RGGWCCY 2 cut(s) 879, 1346
PshAI GACNNNNGTC 1 cut(s) 747
Psp5II RGGWCCY 2 cut(s) 879, 1346
Psp6I CCWGG 5 cut(s) 152, 185, 737, 997, 1348
PspCI CACGTG 1 cut(s) 846
PspEI GGTNACC 1 cut(s) 223
PspFI CCCAGC 1 cut(s) 1586
PspGI CCWGG 5 cut(s) 152, 185, 737, 997, 1348
PspLI CGTACG 1 cut(s) 693
PspN4I GGNNCC 9 cut(s) 184, 397, 717, 880, 881, 931, 967, 1009, 1117
PspOMI GGGCCC 2 cut(s) 929, 1007
PspPI GGNCC 9 cut(s) 715, 879, 929, 930, 995, 1007, 1008, 1116, 1346
PspPPI RGGWCCY 2 cut(s) 879, 1346
PsrI GAACNNNNNNTAC 2 cut(s) 443, 475
PstI CTGCAG 1 cut(s) 1812
PvuII CAGCTG 2 cut(s) 292, 496
RsaI GTAC 6 cut(s) 452, 574, 695, 732, 1262, 1741
RsaNI GTAC 6 cut(s) 451, 573, 694, 731, 1261, 1740
RseI CAYNNNNRTG 2 cut(s) 1478, 1618
SalI GTCGAC 1 cut(s) 435
SapI GCTCTTC 2 cut(s) 947, 1809
SaqAI TTAA 1 cut(s) 1713
SatI GCNGC 9 cut(s) 264, 497, 645, 679, 763, 780, 1108, 1495, 1526
Sau3AI GATC 4 cut(s) 792, 1069, 1483, 1846
Sau96I GGNCC 9 cut(s) 715, 879, 929, 930, 995, 1007, 1008, 1116, 1346
SchI GAGTC 6 cut(s) 225, 432, 484, 533, 538, 716
ScrFI CCNGG 9 cut(s) 154, 187, 221, 259, 541, 739, 933, 999, 1350
SduI GDGCHC 7 cut(s) 179, 240, 933, 970, 993, 1011, 1804
SfaNI GCATC 3 cut(s) 1512, 1569, 1879
SfcI CTRYAG 1 cut(s) 1808
Sfr274I CTCGAG 2 cut(s) 239, 521
SinI GGWCC 4 cut(s) 715, 879, 1116, 1346
SlaI CTCGAG 2 cut(s) 239, 521
SmiMI CAYNNNNRTG 2 cut(s) 1478, 1618
SmlI CTYRAG 2 cut(s) 239, 521
SmoI CTYRAG 2 cut(s) 239, 521
Sse9I AATT 7 cut(s) 19, 59, 139, 638, 1267, 1385, 1574
SspMI CTAG 1 cut(s) 110
StyD4I CCNGG 9 cut(s) 152, 185, 219, 257, 539, 737, 931, 997, 1348
StyI CCWWGG 1 cut(s) 1423
TaaI ACNGT 3 cut(s) 715, 1142, 1238
TaiI ACGT 3 cut(s) 653, 848, 915
TaqI TCGA 7 cut(s) 240, 285, 336, 436, 441, 522, 1056
TasI AATT 7 cut(s) 19, 59, 139, 638, 1267, 1385, 1574
TatI WGTACW 1 cut(s) 1739
TauI GCSGC 3 cut(s) 266, 647, 681
TfiI GAWTC 4 cut(s) 411, 670, 903, 1949
Tru1I TTAA 1 cut(s) 1713
Tru9I TTAA 1 cut(s) 1713
TscAI CASTG 1 cut(s) 1241
TseFI GTSAC 3 cut(s) 196, 223, 835
TseI GCWGC 6 cut(s) 496, 762, 779, 1107, 1494, 1525
Tsp45I GTSAC 3 cut(s) 196, 223, 835
TspDTI ATGAA 6 cut(s) 14, 956, 1291, 1439, 1455, 1536
TspGWI ACGGA 5 cut(s) 422, 465, 506, 718, 915
TspRI CASTG 1 cut(s) 1241
VpaK11BI GGWCC 4 cut(s) 715, 879, 1116, 1346
XapI RAATTY 2 cut(s) 19, 638
XhoI CTCGAG 2 cut(s) 239, 521
XmiI GTMKAC 2 cut(s) 436, 1293
XspI CTAG 1 cut(s) 110
ZraI GACGTC 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.