pycom15g05520

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015555
Physical Location & Seq
Reverse (-)
3286906 .. 3290919
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g05520.1

Sequence Viewer

Length: 2577 bp
ATGTTGCAGACACAGAACAATTTTCATTTTCTCTCACCCTTCCTTTTCTCACTCTTCTTCTTCTTCTTCTTCTCTCTTTCTCTCTCTAACCCAGAAACCACCGCCCTGCTGGCATTCAAATCCTCCGCCGACACCTTCAATTCGCTCTCCTCCTGGGTCGCCTCCTCCGACCCCTGCTCCGGCTCTTGGTTTGGCGTCACTTGCGACCCGGCGAAACGCCGAGTCACCCGAATCGTTCTCGAGAACCTCAACCTTACCGGTTCGGCTCAGCAACTCACCCAACTCGCCCACCTCAAGCTCCTCAGCCTCAACCGCAACCGCCTCTCCTCCTCCCTCAACCTCTCCGCGTGGCACAACCTCAACCACCTGTACCTCTCGCGCAACCGCTTCGAAGGATCCCTTCCGGCCGGAATATCCCGCCTCCGTCACCTCAGGCGGCTCGACTTATCGCACAACCAATTCTCGGGCGAAATCCCACTGACCGAGTTGGCTCAGCTGCCTCACTTGCTAACTCTCCGCCTGGAGTCCAACTCGTTCACCGGCATCCTCGCCTCCGCGCACTCGATTTCTGCGTCCATTTCGGACTTCAATGTTTCGCACAACAACATCTCGGGGAAAATTCCAACGTTTCTGTCCAAATTCGGCGCGACGTCGTTCGCCGGAAACGAGCATCTCTGCGGCAACCCATTGCCGTACAAATGCTCCGATCCAACGGCCGCTAACTTTTCCGTACAATCCCGGAACCGACACGATAGCGTTTTGAGTAACGCCGCGCTGCTCGCGATCAGTATCGTCGGCGCAGCTGTTACGCTGTTAGTGATAATAGTGGCCGCCACGTGGTATCGGCGCACCAAAAGTCGCGGGACCCACAGCGAGACAGCCAACGGACACGGTTACGTGAGCAAGCATTACGGGCCCGGAGAGTTAACGAAGAAGAAAAGTACAACTGGAGTCCAATTGGCCCAGTGTGCCCAGTCTAATGTGAACGGGCCTGTAGGTGGGCCCAGAGAAAGCGAAGACATGGTTGTATTGGAAGGGTACCGGGGCTCCGACAAGGTGGGGGATCTGCTGAAGGCATCGGCGGAGATGCTGGGTAAGGGGAGCGTGGGGACCACTTTCAAAGTCGTGACGGACGGCGGACACAAGGTGGTGGTGAAGAGGGTGAGGGAGAGGAGAAATGGCGGGAAGGAAATCGATGGGTTTTTGAAGCAGATTGGCGGGCTGAGGCACCCCAACATCGCCGCCCTCAGAGCCTACCACAATTCCAACCACGAGCTGCTTTTGGTCTACGATTTATTCCCCGAGGGAAGCTTGCATTCCGCGCTGCATGGAAACCGAGGACCCGGAAGAACGCCGCTTGCTTGGGCCGCAAGATTGAAGCTTGCTGCGGGTTCTGCAGAGGGACTGGCCTTCCTCCACGGCTGCAGCAAATCCAAGCTCTTTCACGGAAACCTCACTTCGTCGAACATATTGGTGGATCGAACGGGAAACGCCTGCCTGGCCGACGTCGGCCTCCAGCAGCTCCTCCCTGCTCCGTTTTCATCATCAACAAGTGCATACAGAGCTCCTGAATTGATGGTGTCCCATAATAAAACTCCGAGAAAGTTCACGCAGAAATGCGATGTTTATAGCTTTGGGGTGGTTCTGTTGGAGATTTTGACGGGGAGGATGGCGGCGGAAGAAGGAGAGACGAATTTGGTGGAGTGGGTTAAGACCGCAGTGCAGAAAGAGTGCAATACTTGGGAAGTGTTGGATTTTGAGCTGTTGGGGGATAGAGCGATGGAGGGCGAAATGTGGGCTCTTTTGGAGGTGGGGTTGCTCTGTGTTACTCCTTTGCCTAAAGATCGCCCGACGATCGGTGTTGTGCATCGGATGATTGAAAATATTCGGAACAAGGCTGTTAGAGAAGATGGTGCCAAGTTTATTTTAGATGAGCTCACTTCTGATTCTTCTTCACCTTCACATTCACATTCTGCAATTGCGAAATTTTATCGAAAAGTTTCAGTCAGACCTTATTTCAAACACATTGCTCTGTTCTTCGTGAGGCTGCGGAACAACTCCTCCACCAACATGGCCTTGTTCTTCTTGTTCTTGAAAAACGTTCTTCTGCACCAGATGAAACTGCGTCTGATCGTACACATTTCCCGCACCCACATTGTACTTAAAGTCCCCCAGACACTGCGCCATCGGCATAACATTGCCGCCGCGGCGGCACAATTCGAGTTGGTAGCGCACGCGGTCGAGTTCAGCCTGGTGCTTGTTGATGGACCAGAGCAGCGAGCGGACGACGGCGTGGCAGCCGCCAACAGGGTCTTTGGCGCGGTGGTCGGCTTCGACAAAGATGGTGGACATGGCGGGGGTTCGGAGGTGGGGAGGGAGGTCGGTGACGAGTTTGGCGAGTCTACTGACGCCGAACAGTTTGAGTGCGTTGTGGAAGTTTGTGGGGTGGTTGGAGGGGAAGTAGGGGGCCAAGGGGCAGTCCTCTGGGCATTTTTTGCGCTGGTGTTTACAAGCGGCGCAACTTCTGGACTTCGTCATTGGGATTTTTTTAGGCTATTTTATCGGTTTGACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

859

Amino Acids

93.87

Weight (kDa)

9.13

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 69 2.1e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 116 - 154 6.6e-06 Leucine rich repeat
Pkinase PF00069 362 - 603 4.1e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 362 - 575 8.4e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015555)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31790
malus_domestica MD15G1058800.v1.1
prunus_persica Prupe.1G411300_v2.0.a1
pyrus_communis pycom08g05190 pycom15g05520
rosa_chinensis RchiOBHm_Chr6g0311031
rosa_laevigata RLG00000010441
rosa_multiflora Rmu_sc0012235.1_g000006 Rmu_sc0014619.1_g000002
rosa_roxburghii Rroxscaffold_7G00158090
rosa_rugosa Rorug06G0384900
rosa_samantha Rh6AG498700 Rh6BG509300 Rh6CG515100 Rh6DG501700
rosa_wichuraiana Rw6G043430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 653, 1509
Acc65I GGTACC 1 cut(s) 1038
AccB1I GGYRCC 3 cut(s) 1038, 1227, 1913
AccBSI CCGCTC 1 cut(s) 2282
AccI GTMKAC 2 cut(s) 1287, 2402
AclI AACGTT 2 cut(s) 626, 2100
AclWI GGATC 5 cut(s) 390, 403, 701, 1071, 1485
AcoI YGGCCR 4 cut(s) 405, 714, 828, 1500
AcsI RAATTY 4 cut(s) 618, 638, 1693, 1985
AcuI CTGAAG 1 cut(s) 1091
AcvI CACGTG 1 cut(s) 837
AcyI GRCGYC 4 cut(s) 195, 650, 1506, 2409
AdeI CACNNNGTG 1 cut(s) 1147
AfaI GTAC 7 cut(s) 371, 695, 732, 943, 1040, 2136, 2160
AfiI CCNNNNNNNGG 7 cut(s) 179, 186, 463, 837, 998, 1856, 2307
AgeI ACCGGT 1 cut(s) 257
AgsI TTSAA 9 cut(s) 118, 139, 589, 1120, 1207, 1378, 1880, 2020, 2095
AjnI CCWGG 4 cut(s) 152, 519, 1497, 2250
AloI GAACNNNNNNTCC 2 cut(s) 2045, 2077
Alw21I GWGCWC 2 cut(s) 1567, 1938
Alw26I GTCTC 2 cut(s) 869, 1682
AlwI GGATC 5 cut(s) 390, 403, 701, 1071, 1485
AlwNI CAGNNNCTG 1 cut(s) 2179
Ama87I CYCGRG 4 cut(s) 239, 463, 610, 1301
ApaI GGGCCC 2 cut(s) 918, 1005
ApoI RAATTY 4 cut(s) 618, 638, 1693, 1985
AsiGI ACCGGT 1 cut(s) 257
Asp700I GAANNNNTTC 2 cut(s) 1884, 1999
Asp718I GGTACC 1 cut(s) 1038
AsuC2I CCSGG 5 cut(s) 209, 739, 918, 1043, 1344
AsuHPI GGTGA 9 cut(s) 27, 217, 268, 419, 529, 1165, 1174, 1947, 2396
AsuII TTCGAA 1 cut(s) 390
AvaI CYCGRG 4 cut(s) 239, 463, 610, 1301
AvaII GGWCC 4 cut(s) 864, 1110, 1340, 2267
AxyI CCTNAGG 1 cut(s) 431
BaeGI GKGCMC 3 cut(s) 918, 973, 1005
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 3 cut(s) 1038, 1227, 1913
BanII GRGCYC 6 cut(s) 918, 1005, 1049, 1567, 1801, 1938
BauI CACGAG 1 cut(s) 1271
BbrPI CACGTG 1 cut(s) 837
BbsI GAAGAC 1 cut(s) 1023
Bbv12I GWGCWC 2 cut(s) 1567, 1938
BbvCI CCTCAGC 2 cut(s) 302, 1223
BccI CCATC 8 cut(s) 1190, 1570, 1663, 1774, 1904, 2193, 2258, 2336
BceAI ACGGC 5 cut(s) 676, 729, 1150, 1435, 2305
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 4 cut(s) 154, 521, 1499, 2252
BcnI CCSGG 5 cut(s) 209, 739, 918, 1043, 1344
BcoDI GTCTC 2 cut(s) 869, 1682
BfmI CTRYAG 3 cut(s) 993, 1395, 1423
BglI GCCNNNNNGGC 4 cut(s) 110, 1499, 2207, 2210
BlpI GCTNAGC 2 cut(s) 267, 492
Bme1390I CCNGG 9 cut(s) 154, 209, 521, 739, 918, 1043, 1344, 1499, 2252
Bme18I GGWCC 4 cut(s) 864, 1110, 1340, 2267
BmeT110I CYCGRG 4 cut(s) 239, 463, 610, 1301
BmrFI CCNGG 9 cut(s) 154, 209, 521, 739, 918, 1043, 1344, 1499, 2252
BmrI ACTGGG 2 cut(s) 958, 967
BmsI GCATC 5 cut(s) 552, 679, 1077, 1085, 1876
BmuI ACTGGG 2 cut(s) 958, 967
BpiI GAAGAC 1 cut(s) 1023
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 3 cut(s) 542, 969, 1499
Bpu10I CCTNAGC 2 cut(s) 302, 1223
Bpu1102I GCTNAGC 2 cut(s) 267, 492
Bpu14I TTCGAA 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 5 cut(s) 209, 739, 918, 1043, 1344
Bsa29I ATCGAT 1 cut(s) 1194
BsaAI YACGTR 2 cut(s) 837, 898
BsaBI GATNNNNATC 1 cut(s) 788
BsaHI GRCGYC 4 cut(s) 195, 650, 1506, 2409
BsaJI CCNNGG 7 cut(s) 153, 1042, 1302, 1336, 1417, 2205, 2470
BsaWI WCCGGW 1 cut(s) 257
Bsc4I CCNNNNNNNGG 7 cut(s) 179, 186, 463, 837, 998, 1856, 2307
Bse118I RCCGGY 2 cut(s) 257, 539
Bse1I ACTGG 4 cut(s) 952, 964, 973, 1410
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 3 cut(s) 686, 2025, 2196
Bse8I GATNNNNATC 1 cut(s) 788
BseBI CCWGG 4 cut(s) 154, 521, 1499, 2252
BseCI ATCGAT 1 cut(s) 1194
BseDI CCNNGG 7 cut(s) 153, 1042, 1302, 1336, 1417, 2205, 2470
BseGI GGATG 3 cut(s) 543, 1674, 1878
BseJI GATNNNNATC 1 cut(s) 788
BseLI CCNNNNNNNGG 7 cut(s) 179, 186, 463, 837, 998, 1856, 2307
BseMI GCAATG 3 cut(s) 686, 2025, 2196
BseMII CTCAG 6 cut(s) 281, 316, 445, 506, 1214, 1261
BseNI ACTGG 4 cut(s) 952, 964, 973, 1410
BseRI GAGGAG 8 cut(s) 139, 154, 290, 316, 319, 1186, 1514, 2050
BseSI GKGCMC 3 cut(s) 918, 973, 1005
BseX3I CGGCCG 2 cut(s) 405, 714
BseYI CCCAGC 1 cut(s) 1090
BsgI GTGCAG 2 cut(s) 1742, 2093
Bsh1285I CGRYCG 4 cut(s) 408, 717, 1857, 2241
BshNI GGYRCC 3 cut(s) 1038, 1227, 1913
BshTI ACCGGT 1 cut(s) 257
BshVI ATCGAT 1 cut(s) 1194
BsiEI CGRYCG 4 cut(s) 408, 717, 1857, 2241
BsiHKAI GWGCWC 2 cut(s) 1567, 1938
BsiHKCI CYCGRG 4 cut(s) 239, 463, 610, 1301
BslFI GGGAC 5 cut(s) 877, 1123, 1416, 1567, 2153
BslI CCNNNNNNNGG 7 cut(s) 179, 186, 463, 837, 998, 1856, 2307
BsmAI GTCTC 2 cut(s) 869, 1682
BsmBI CGTCTC 1 cut(s) 1682
BsmFI GGGAC 5 cut(s) 877, 1123, 1416, 1567, 2153
BsmI GAATGC 2 cut(s) 113, 1315
BsoBI CYCGRG 4 cut(s) 239, 463, 610, 1301
Bsp119I TTCGAA 1 cut(s) 390
Bsp120I GGGCCC 2 cut(s) 914, 1001
Bsp1286I GDGCHC 7 cut(s) 918, 973, 1005, 1049, 1567, 1801, 1938
Bsp143I GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
Bsp1720I GCTNAGC 2 cut(s) 267, 492
Bsp68I TCGCGA 1 cut(s) 782
BspCNI CTCAG 6 cut(s) 280, 315, 444, 505, 1215, 1260
BspDI ATCGAT 1 cut(s) 1194
BspMAI CTGCAG 2 cut(s) 1399, 1427
BspPI GGATC 5 cut(s) 390, 403, 701, 1071, 1485
BspT104I TTCGAA 1 cut(s) 390
BspT107I GGYRCC 3 cut(s) 1038, 1227, 1913
BsrBI CCGCTC 1 cut(s) 2282
BsrDI GCAATG 3 cut(s) 686, 2025, 2196
BsrFI RCCGGY 2 cut(s) 257, 539
BsrI ACTGG 4 cut(s) 952, 964, 973, 1410
BssAI RCCGGY 2 cut(s) 257, 539
BssECI CCNNGG 7 cut(s) 153, 1042, 1302, 1336, 1417, 2205, 2470
BssMI GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
BssNI GRCGYC 4 cut(s) 195, 650, 1506, 2409
BssSI CACGAG 1 cut(s) 1271
BssT1I CCWWGG 1 cut(s) 2470
Bst2BI CACGAG 1 cut(s) 1271
Bst2UI CCWGG 4 cut(s) 154, 521, 1499, 2252
Bst4CI ACNGT 2 cut(s) 893, 2418
Bst6I CTCTTC 2 cut(s) 59, 1151
BstACI GRCGYC 4 cut(s) 195, 650, 1506, 2409
BstAPI GCANNNNNTGC 1 cut(s) 2495
BstBAI YACGTR 2 cut(s) 837, 898
BstBI TTCGAA 1 cut(s) 390
BstDEI CTNAG 6 cut(s) 267, 302, 431, 492, 1223, 1247
BstDSI CCRYGG 2 cut(s) 1417, 2205
BstF5I GGATG 3 cut(s) 543, 1674, 1878
BstKTI GATC 8 cut(s) 398, 709, 786, 1066, 1480, 1846, 1857, 2133
BstMAI GTCTC 2 cut(s) 869, 1682
BstMBI GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
BstMCI CGRYCG 4 cut(s) 408, 717, 1857, 2241
BstNI CCWGG 4 cut(s) 154, 521, 1499, 2252
BstSCI CCNGG 9 cut(s) 152, 207, 519, 737, 916, 1041, 1342, 1497, 2250
BstSFI CTRYAG 3 cut(s) 993, 1395, 1423
BstSLI GKGCMC 3 cut(s) 918, 973, 1005
BstV2I GAAGAC 1 cut(s) 1023
BstX2I RGATCY 2 cut(s) 395, 1063
BstXI CCANNNNNNTGG 1 cut(s) 2071
BstYI RGATCY 2 cut(s) 395, 1063
BstZI CGGCCG 2 cut(s) 405, 714
Bsu15I ATCGAT 1 cut(s) 1194
Bsu36I CCTNAGG 1 cut(s) 431
BsuTUI ATCGAT 1 cut(s) 1194
BtgI CCRYGG 2 cut(s) 1417, 2205
BtgZI GCGATG 3 cut(s) 1222, 1635, 1793
BtsCI GGATG 3 cut(s) 543, 1674, 1878
BtsI GCAGTG 2 cut(s) 1725, 2177
BtsIMutI CAGTG 4 cut(s) 476, 971, 1725, 2177
BtuMI TCGCGA 1 cut(s) 782
CaiI CAGNNNCTG 1 cut(s) 2179
Cfr10I RCCGGY 2 cut(s) 257, 539
Cfr42I CCGCGG 1 cut(s) 2208
ClaI ATCGAT 1 cut(s) 1194
CseI GACGC 4 cut(s) 184, 561, 2114, 2417
Csp6I GTAC 7 cut(s) 370, 694, 731, 942, 1039, 2135, 2159
CspAI ACCGGT 1 cut(s) 257
CspCI CAANNNNNGTGG 2 cut(s) 2326, 2361
CviAII CATG 4 cut(s) 1021, 1328, 2071, 2351
CviQI GTAC 7 cut(s) 370, 694, 731, 942, 1039, 2135, 2159
DdeI CTNAG 6 cut(s) 267, 302, 431, 492, 1223, 1247
DpnI GATC 8 cut(s) 397, 708, 785, 1065, 1479, 1845, 1856, 2132
DpnII GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
DraIII CACNNNGTG 1 cut(s) 1147
EaeI YGGCCR 4 cut(s) 405, 714, 828, 1500
EagI CGGCCG 2 cut(s) 405, 714
Eam1104I CTCTTC 2 cut(s) 59, 1151
EarI CTCTTC 2 cut(s) 59, 1151
EciI GGCGGA 5 cut(s) 115, 506, 1097, 1152, 1691
Ecl136II GAGCTC 2 cut(s) 1565, 1936
EclXI CGGCCG 2 cut(s) 405, 714
Eco130I CCWWGG 1 cut(s) 2470
Eco24I GRGCYC 6 cut(s) 918, 1005, 1049, 1567, 1801, 1938
Eco47I GGWCC 4 cut(s) 864, 1110, 1340, 2267
Eco52I CGGCCG 2 cut(s) 405, 714
Eco53kI GAGCTC 2 cut(s) 1565, 1936
Eco57I CTGAAG 1 cut(s) 1091
Eco72I CACGTG 1 cut(s) 837
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 4 cut(s) 239, 463, 610, 1301
EcoICRI GAGCTC 2 cut(s) 1565, 1936
EcoO109I RGGNCCY 2 cut(s) 864, 1340
EcoRII CCWGG 4 cut(s) 152, 519, 1497, 2250
EcoT14I CCWWGG 1 cut(s) 2470
EcoT38I GRGCYC 6 cut(s) 918, 1005, 1049, 1567, 1801, 1938
ErhI CCWWGG 1 cut(s) 2470
Esp3I CGTCTC 1 cut(s) 1682
FaeI CATG 4 cut(s) 1024, 1331, 2074, 2354
FaiI YATR 9 cut(s) 1022, 1329, 1469, 1558, 1587, 1629, 2072, 2193, 2352
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 5 cut(s) 877, 1123, 1416, 1567, 2153
FatI CATG 4 cut(s) 1020, 1327, 2070, 2350
FauI CCCGC 7 cut(s) 425, 854, 1175, 1211, 1381, 2153, 2348
FblI GTMKAC 2 cut(s) 1287, 2402
FokI GGATG 3 cut(s) 530, 1681, 1885
FriOI GRGCYC 6 cut(s) 918, 1005, 1049, 1567, 1801, 1938
GsaI CCCAGC 1 cut(s) 1094
GsuI CTGGAG 3 cut(s) 542, 969, 1499
HgaI GACGC 4 cut(s) 184, 561, 2114, 2417
Hin1I GRCGYC 4 cut(s) 195, 650, 1506, 2409
Hin1II CATG 4 cut(s) 1024, 1331, 2074, 2354
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HindIII AAGCTT 2 cut(s) 1309, 1379
HinfI GANTC 6 cut(s) 222, 231, 524, 951, 1946, 2399
HpaI GTTAAC 1 cut(s) 927
HphI GGTGA 9 cut(s) 27, 217, 268, 419, 529, 1165, 1174, 1947, 2396
Hpy166II GTNNAC 9 cut(s) 537, 927, 985, 1288, 1608, 2137, 2348, 2403, 2508
Hpy188III TCNNGA 8 cut(s) 239, 241, 781, 1126, 1568, 2041, 2092, 2526
Hpy8I GTNNAC 9 cut(s) 537, 927, 985, 1288, 1608, 2137, 2348, 2403, 2508
Hpy99I CGWCG 8 cut(s) 652, 655, 797, 1465, 1508, 1511, 1855, 2291
HpyCH4III ACNGT 2 cut(s) 893, 2418
HpyCH4IV ACGT 6 cut(s) 626, 650, 836, 897, 1506, 2100
HpyF3I CTNAG 6 cut(s) 267, 302, 431, 492, 1223, 1247
HpySE526I ACGT 6 cut(s) 626, 650, 836, 897, 1506, 2100
Hsp92I GRCGYC 4 cut(s) 195, 650, 1506, 2409
Hsp92II CATG 4 cut(s) 1024, 1331, 2074, 2354
KflI GGGWCCC 1 cut(s) 864
KpnI GGTACC 1 cut(s) 1042
KspAI GTTAAC 1 cut(s) 927
KspI CCGCGG 1 cut(s) 2208
Kzo9I GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
LmnI GCTCC 8 cut(s) 182, 303, 707, 1052, 1101, 1527, 1537, 1570
LweI GCATC 5 cut(s) 552, 679, 1077, 1085, 1876
MaeII ACGT 6 cut(s) 626, 650, 836, 897, 1506, 2100
MaeIII GTNAC 9 cut(s) 196, 223, 425, 764, 805, 893, 1126, 1825, 2384
MalI GATC 8 cut(s) 397, 708, 785, 1065, 1479, 1845, 1856, 2132
MbiI CCGCTC 1 cut(s) 2282
MboI GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
MfeI CAATTG 2 cut(s) 956, 1977
MflI RGATCY 2 cut(s) 395, 1063
MhlI GDGCHC 7 cut(s) 918, 973, 1005, 1049, 1567, 1801, 1938
MlyI GAGTC 4 cut(s) 231, 533, 960, 2408
MmeI TCCRAC 9 cut(s) 192, 552, 647, 734, 1074, 1290, 1629, 1731, 2431
MroXI GAANNNNTTC 2 cut(s) 1884, 1999
MseI TTAA 3 cut(s) 926, 1710, 2163
MslI CAYNNNNRTG 2 cut(s) 1472, 2069
MspA1I CMGCKG 3 cut(s) 496, 803, 2207
MspR9I CCNGG 9 cut(s) 154, 209, 521, 739, 918, 1043, 1344, 1499, 2252
MunI CAATTG 2 cut(s) 956, 1977
Mva1269I GAATGC 2 cut(s) 113, 1315
MvaI CCWGG 4 cut(s) 154, 521, 1499, 2252
NciI CCSGG 5 cut(s) 209, 739, 918, 1043, 1344
NdeII GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
NlaIII CATG 4 cut(s) 1024, 1331, 2074, 2354
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 5 cut(s) 196, 223, 425, 1126, 2384
NruI TCGCGA 1 cut(s) 782
NspV TTCGAA 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 239
PcsI WCGNNNNNNNCGW 4 cut(s) 165, 569, 663, 2394
PctI GAATGC 2 cut(s) 113, 1315
PdmI GAANNNNTTC 2 cut(s) 1884, 1999
PfeI GAWTC 2 cut(s) 231, 1946
PflFI GACNNNGTC 1 cut(s) 2532
PfoI TCCNGGA 1 cut(s) 737
PinAI ACCGGT 1 cut(s) 257
Ple19I CGATCG 1 cut(s) 1857
PleI GAGTC 4 cut(s) 230, 532, 959, 2407
PmaCI CACGTG 1 cut(s) 837
PmlI CACGTG 1 cut(s) 837
PpsI GAGTC 4 cut(s) 230, 532, 959, 2407
Ppu21I YACGTR 2 cut(s) 837, 898
PpuMI RGGWCCY 2 cut(s) 864, 1340
Psp124BI GAGCTC 2 cut(s) 1567, 1938
Psp1406I AACGTT 2 cut(s) 626, 2100
Psp5II RGGWCCY 2 cut(s) 864, 1340
Psp6I CCWGG 4 cut(s) 152, 519, 1497, 2250
PspCI CACGTG 1 cut(s) 837
PspFI CCCAGC 1 cut(s) 1090
PspGI CCWGG 4 cut(s) 152, 519, 1497, 2250
PspOMI GGGCCC 2 cut(s) 914, 1001
PspPPI RGGWCCY 2 cut(s) 864, 1340
PstI CTGCAG 2 cut(s) 1399, 1427
PstNI CAGNNNCTG 1 cut(s) 2179
PsuI RGATCY 2 cut(s) 395, 1063
PsyI GACNNNGTC 1 cut(s) 2532
PvuI CGATCG 1 cut(s) 1857
PvuII CAGCTG 2 cut(s) 496, 803
RruI TCGCGA 1 cut(s) 782
RsaI GTAC 7 cut(s) 371, 695, 732, 943, 1040, 2136, 2160
RsaNI GTAC 7 cut(s) 370, 694, 731, 942, 1039, 2135, 2159
RseI CAYNNNNRTG 2 cut(s) 1472, 2069
SacI GAGCTC 2 cut(s) 1567, 1938
SacII CCGCGG 1 cut(s) 2208
SaqAI TTAA 3 cut(s) 926, 1710, 2163
Sau3AI GATC 8 cut(s) 395, 706, 783, 1063, 1477, 1843, 1854, 2130
SchI GAGTC 4 cut(s) 231, 533, 960, 2408
ScrFI CCNGG 9 cut(s) 154, 209, 521, 739, 918, 1043, 1344, 1499, 2252
SduI GDGCHC 7 cut(s) 918, 973, 1005, 1049, 1567, 1801, 1938
SfaNI GCATC 5 cut(s) 552, 679, 1077, 1085, 1876
SfcI CTRYAG 3 cut(s) 993, 1395, 1423
Sfr274I CTCGAG 1 cut(s) 239
Sfr303I CCGCGG 1 cut(s) 2208
SfuI TTCGAA 1 cut(s) 390
SgrBI CCGCGG 1 cut(s) 2208
SinI GGWCC 4 cut(s) 864, 1110, 1340, 2267
SlaI CTCGAG 1 cut(s) 239
SmiMI CAYNNNNRTG 2 cut(s) 1472, 2069
SmlI CTYRAG 2 cut(s) 239, 293
SmoI CTYRAG 2 cut(s) 239, 293
SspI AATATT 1 cut(s) 1885
SstI GAGCTC 2 cut(s) 1567, 1938
StyD4I CCNGG 9 cut(s) 152, 207, 519, 737, 916, 1041, 1342, 1497, 2250
StyI CCWWGG 1 cut(s) 2470
TaaI ACNGT 2 cut(s) 893, 2418
TaiI ACGT 6 cut(s) 629, 653, 839, 900, 1509, 2103
TaqII GACCGA 1 cut(s) 497
TatI WGTACW 2 cut(s) 941, 2158
TfiI GAWTC 2 cut(s) 231, 1946
Tru1I TTAA 3 cut(s) 926, 1710, 2163
Tru9I TTAA 3 cut(s) 926, 1710, 2163
TscAI CASTG 4 cut(s) 483, 971, 1725, 2184
TseFI GTSAC 5 cut(s) 196, 223, 425, 1126, 2384
Tsp45I GTSAC 5 cut(s) 196, 223, 425, 1126, 2384
TspDTI ATGAA 3 cut(s) 14, 1530, 2132
TspGWI ACGGA 6 cut(s) 413, 718, 900, 1145, 1461, 1524
TspRI CASTG 4 cut(s) 483, 971, 1725, 2184
Tth111I GACNNNGTC 1 cut(s) 2532
VpaK11BI GGWCC 4 cut(s) 864, 1110, 1340, 2267
XapI RAATTY 4 cut(s) 618, 638, 1693, 1985
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XhoI CTCGAG 1 cut(s) 239
XmiI GTMKAC 2 cut(s) 1287, 2402
XmnI GAANNNNTTC 2 cut(s) 1884, 1999
ZraI GACGTC 2 cut(s) 651, 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.