Rh6BG509300

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015555
Physical Location & Seq
Forward (+)
70573288 .. 70575264
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG509300.1

Sequence Viewer

Length: 1977 bp
ATGCTGCGAAACCAGAATCTCCTCTCTATCTTCTTCTTCACACTCTTCTTCTCTCTTTCTCTCTCCAACCCAAGCCCAGAAGATAAAGCCGCCTTGCTCGCATTCAAAGCCTCCTCTGACCGCTCCAACTCCCTCGCCTCCTGGGCCGAATCCACCGACCCCTGCGCCGGGTCATGGCTCGGCGTCACGTGCAACCCGAAAACCCGTCGGGTCACCAAGCTTGTGCTCGAGAATCTCAACCTCACCGGTTCGATTCAGCCGCTCACCGGACTCGACCGCCTCACCGTCCTCAGCCTCAACCAAAACCTCCTCTCCTCCTCCCTCAACCTCTCCTCCTGGCCCCACCTCAACCACCTCTACCTCTCTCACAATAACCTCACCGGAGCCATCCCCGCCGGAATTTCCAGCCTCCGCCACCTCCGCCGACTCGACCTCTCCCACAACCAACTCTCCGGCGAAATACCCGTCGCCGAGCTGACTCGGTTGTCTAAGCTGTTAACTCTGAGGCTCGAGTCCAACTCGTTCACCGGTTTCGGCGGTTCGCCTCCCGACGCGTCCTCTGCTCCGTTCACGGATTTCAATGTGTCCAACAACGAACTCGCCGGGAAGATTCCTCTGTTTCTGGAGAAGTTCGGTGCGGCGTCGTTTTTGGGAAACGAGCAGCTCTGCGGGAAGCCGCTGCCGCACGATTGCCCCGATCCGACGGCCGTTAAGCCGCCGTCCCTACCACCGGCCGAACCCGAAGCCAAACCGCACTCGAAACAAGCTGTTCTGATCGGCGTCAGCCTCATGCTTGGGTTTCTGGTGATCGCCGGCGCCGTCATGCGGTGGCGGCAGAGGAAGAGGAGCGATGTTGTTGTCGTCGGCGGCGGGAACCCCAAGGTGGCCAACGGATGCAGTTACGTGAAAAGGCACTACGGGTCCAGGGAATTAAAGAGCGGCGGGTCCCACAGAAACGGTTATGGGCCGCGTGGGCGTAACGGGTCCGTAAGTGGCGGGCCCAGGGAAAGCGATGAGATGGTGGTGCTCGAGGGATGCCACCCTGGTGTCGTGAAGGTGGGCGATCTGCTGAGGGCGTCGGCAGAGCTGCTCGGAAAGGGAAGCGTGGGGGCCACTTACAAGATCATGATGAACGGCTGCGATGACATGGTGGCGGTGAAGAGGGTGAGGGAGAGGAGAGTTCACGGCAAGGAAGTCGAAGCGTGGTTGAGAATTGTCGGCGAATTGAAACACTCGAACATTGTGAGCCTGAGAGCCTGCCACAACTCCAACTGCGAGCTTCTTTTGGTGTATGATTTTCTGCCTAACGGAAGCCTACATTCTCTGTTGCACGGGTTTAGAGGACCGGGGAGGACACCGTTGGATTGGAGTACAAGACTGAAACTAGCTTGGGGCTGTGCACAAGGACTTGCTTTCATCCACGGGTGTGAGAAAGGCAAGCTCTATCACGGACACGTTAACTCGTCCAACATTTTAATCGATCACATGGGGAATGCTTGTGTTGCTGACAATGGCCTCCAACAGCTCTTGGTTGCTCAAAGTTCGTCCTACAATGCCTACAAAGCCCCGGAGTTGATCATGTCGAGTAGTTTTAGTCCTAACAAGTACACTCGCAAGTGCGATGTGTATAGCTTTGGGGTGGTTTTGATGGAGATTTTGACGGGGAGGATGGCGGGCGAGGAAGGAGAAATGAGCTTGGTGGAGTGGGTTAACAGGGCGGTGAAGAAAGAGTGTAGTACATGGGAACTTTTTGATTTCGAGCTGTTGAGTGACAAGGAGATGGAGGAGGAAATGTGGGCGCTTTTGCAGGTGGGATTGCTTTGCACAGCCGCGCAGCCTAAAGATCGTCCAACGATGAATATGGTGCGCAATATGATCGAGGACATTAGGAACAAGGGTGTTAGAGAAGATGGGACCAAGTCTATTCTAGATGACATTTCGTTTGATACTTCTTCTCCTTCACAAAGCACTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

658

Amino Acids

71.86

Weight (kDa)

8.51

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 65 4.9e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 111 - 150 3.2e-08 Leucine rich repeat
LRR_4 PF12799 114 - 150 8.7e-07 Leucine Rich repeats (2 copies)
Pkinase PF00069 362 - 623 1.3e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 362 - 624 1.9e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015555)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31790
malus_domestica MD15G1058800.v1.1
prunus_persica Prupe.1G411300_v2.0.a1
pyrus_communis pycom08g05190 pycom15g05520
rosa_chinensis RchiOBHm_Chr6g0311031
rosa_laevigata RLG00000010441
rosa_multiflora Rmu_sc0012235.1_g000006 Rmu_sc0014619.1_g000002
rosa_roxburghii Rroxscaffold_7G00158090
rosa_rugosa Rorug06G0384900
rosa_samantha Rh6AG498700 Rh6BG509300 Rh6CG515100 Rh6DG501700
rosa_wichuraiana Rw6G043430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1798
AasI GACNNNNNNGTC 1 cut(s) 484
Acc16I TGCGCA 1 cut(s) 1868
Acc36I ACCTGC 1 cut(s) 1798
AccB1I GGYRCC 1 cut(s) 815
AccBSI CCGCTC 3 cut(s) 123, 262, 939
AccII CGCG 3 cut(s) 554, 970, 1832
AclWI GGATC 1 cut(s) 692
AcoI YGGCCR 3 cut(s) 705, 732, 885
AcsI RAATTY 1 cut(s) 399
AcvI CACGTG 1 cut(s) 189
AcyI GRCGYC 5 cut(s) 183, 641, 780, 816, 1076
AfaI GTAC 3 cut(s) 1372, 1607, 1738
AfiI CCNNNNNNNGG 7 cut(s) 167, 168, 174, 266, 730, 825, 883
AflIII ACRYGT 2 cut(s) 552, 1453
AgeI ACCGGT 2 cut(s) 245, 527
AgsI TTSAA 3 cut(s) 106, 580, 1228
AjnI CCWGG 5 cut(s) 140, 335, 923, 1001, 1042
AleI CACNNNNGTG 2 cut(s) 1044, 1425
Alw21I GWGCWC 3 cut(s) 228, 1029, 1402
Alw44I GTGCAC 1 cut(s) 1398
AlwI GGATC 1 cut(s) 692
Ama87I CYCGRG 3 cut(s) 227, 509, 1028
AoxI GGCC 9 cut(s) 144, 338, 705, 732, 885, 965, 998, 1110, 1513
ApaI GGGCCC 1 cut(s) 1002
ApaLI GTGCAC 1 cut(s) 1398
ApeKI GCWGC 6 cut(s) 4, 661, 679, 1087, 1137, 1834
ApoI RAATTY 1 cut(s) 399
ArsI GACNNNNNNTTYG 4 cut(s) 450, 482, 1925, 1957
AsiGI ACCGGT 2 cut(s) 245, 527
AspLEI GCGC 5 cut(s) 167, 818, 1801, 1834, 1869
AsuC2I CCSGG 4 cut(s) 169, 604, 1347, 1568
AvaI CYCGRG 3 cut(s) 227, 509, 1028
AvaII GGWCC 5 cut(s) 921, 945, 984, 1343, 1914
BaeGI GKGCMC 2 cut(s) 1002, 1402
BalI TGGCCA 1 cut(s) 887
BanI GGYRCC 1 cut(s) 815
BanII GRGCYC 1 cut(s) 1002
BbrPI CACGTG 1 cut(s) 189
Bbv12I GWGCWC 3 cut(s) 228, 1029, 1402
BbvCI CCTCAGC 2 cut(s) 290, 1070
BbvI GCAGC 5 cut(s) 666, 673, 1074, 1124, 1846
BccI CCATC 6 cut(s) 395, 1012, 1642, 1663, 1774, 1904
BceAI ACGGC 6 cut(s) 692, 703, 720, 803, 1150, 1201
BcgI CGANNNNNNTGC 4 cut(s) 1177, 1211, 1858, 1892
BciT130I CCWGG 5 cut(s) 142, 337, 925, 1003, 1044
BclI TGATCA 1 cut(s) 1575
BcnI CCSGG 4 cut(s) 169, 604, 1347, 1568
BfaI CTAG 2 cut(s) 1385, 1928
BfoI RGCGCY 2 cut(s) 819, 1802
BfuAI ACCTGC 1 cut(s) 1798
BglI GCCNNNNNGGC 2 cut(s) 143, 973
Bme1390I CCNGG 9 cut(s) 142, 169, 337, 604, 925, 1003, 1044, 1347, 1568
Bme18I GGWCC 5 cut(s) 921, 945, 984, 1343, 1914
BmeT110I CYCGRG 3 cut(s) 227, 509, 1028
BmrFI CCNGG 9 cut(s) 142, 169, 337, 604, 925, 1003, 1044, 1347, 1568
BmsI GCATC 2 cut(s) 884, 1025
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpmI CTGGAG 1 cut(s) 644
Bpu10I CCTNAGC 2 cut(s) 290, 1070
BpuMI CCSGG 4 cut(s) 169, 604, 1347, 1568
Bsa29I ATCGAT 1 cut(s) 1479
BsaAI YACGTR 2 cut(s) 189, 904
BsaHI GRCGYC 5 cut(s) 183, 641, 780, 816, 1076
BsaJI CCNNGG 9 cut(s) 141, 879, 924, 1001, 1002, 1042, 1346, 1420, 1566
BsaWI WCCGGW 4 cut(s) 245, 266, 380, 527
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 168, 174, 266, 730, 825, 883
Bse118I RCCGGY 4 cut(s) 245, 527, 730, 812
BseBI CCWGG 5 cut(s) 142, 337, 925, 1003, 1044
BseCI ATCGAT 1 cut(s) 1479
BseDI CCNNGG 9 cut(s) 141, 879, 924, 1001, 1002, 1042, 1346, 1420, 1566
BseGI GGATG 5 cut(s) 387, 899, 1040, 1416, 1674
BseLI CCNNNNNNNGG 7 cut(s) 167, 168, 174, 266, 730, 825, 883
BseMII CTCAG 4 cut(s) 304, 494, 1061, 1241
BseRI GAGGAG 9 cut(s) 11, 103, 299, 304, 307, 322, 859, 1189, 1799
BseSI GKGCMC 2 cut(s) 1002, 1402
BseX3I CGGCCG 2 cut(s) 705, 732
BseXI GCAGC 5 cut(s) 666, 673, 1074, 1124, 1846
Bsh1236I CGCG 3 cut(s) 554, 970, 1832
Bsh1285I CGRYCG 3 cut(s) 277, 708, 735
BshFI GGCC 9 cut(s) 146, 340, 707, 734, 887, 967, 1000, 1112, 1515
BshNI GGYRCC 1 cut(s) 815
BshTI ACCGGT 2 cut(s) 245, 527
BshVI ATCGAT 1 cut(s) 1479
BsiEI CGRYCG 3 cut(s) 277, 708, 735
BsiHKAI GWGCWC 3 cut(s) 228, 1029, 1402
BsiHKCI CYCGRG 3 cut(s) 227, 509, 1028
BslFI GGGAC 3 cut(s) 706, 931, 1927
BslI CCNNNNNNNGG 7 cut(s) 167, 168, 174, 266, 730, 825, 883
BsmFI GGGAC 3 cut(s) 706, 931, 1927
BsmI GAATGC 2 cut(s) 101, 1498
BsnI GGCC 9 cut(s) 146, 340, 707, 734, 887, 967, 1000, 1112, 1515
BsoBI CYCGRG 3 cut(s) 227, 509, 1028
Bsp120I GGGCCC 1 cut(s) 998
Bsp1286I GDGCHC 4 cut(s) 228, 1002, 1029, 1402
Bsp143I GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
BspANI GGCC 9 cut(s) 146, 340, 707, 734, 887, 967, 1000, 1112, 1515
BspCNI CTCAG 4 cut(s) 303, 495, 1062, 1242
BspDI ATCGAT 1 cut(s) 1479
BspFNI CGCG 3 cut(s) 554, 970, 1832
BspHI TCATGA 1 cut(s) 1125
BspMI ACCTGC 1 cut(s) 1798
BspPI GGATC 1 cut(s) 692
BspT107I GGYRCC 1 cut(s) 815
BsrBI CCGCTC 3 cut(s) 123, 262, 939
BsrFI RCCGGY 4 cut(s) 245, 527, 730, 812
BssAI RCCGGY 4 cut(s) 245, 527, 730, 812
BssECI CCNNGG 9 cut(s) 141, 879, 924, 1001, 1002, 1042, 1346, 1420, 1566
BssMI GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
BssNI GRCGYC 5 cut(s) 183, 641, 780, 816, 1076
BssT1I CCWWGG 1 cut(s) 879
Bst2UI CCWGG 5 cut(s) 142, 337, 925, 1003, 1044
Bst4CI ACNGT 3 cut(s) 286, 959, 1359
Bst6I CTCTTC 3 cut(s) 50, 836, 1154
BstACI GRCGYC 5 cut(s) 183, 641, 780, 816, 1076
BstBAI YACGTR 2 cut(s) 189, 904
BstC8I GCNNGC 7 cut(s) 99, 814, 998, 1258, 1277, 1439, 1675
BstDEI CTNAG 5 cut(s) 290, 489, 503, 1070, 1250
BstDSI CCRYGG 1 cut(s) 1420
BstEII GGTNACC 1 cut(s) 211
BstF5I GGATG 5 cut(s) 387, 899, 1040, 1416, 1674
BstFNI CGCG 3 cut(s) 554, 970, 1832
BstH2I RGCGCY 2 cut(s) 819, 1802
BstHHI GCGC 5 cut(s) 167, 818, 1801, 1834, 1869
BstKTI GATC 9 cut(s) 700, 777, 810, 1066, 1125, 1483, 1578, 1846, 1878
BstMBI GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
BstMCI CGRYCG 3 cut(s) 277, 708, 735
BstNI CCWGG 5 cut(s) 142, 337, 925, 1003, 1044
BstPI GGTNACC 1 cut(s) 211
BstSCI CCNGG 9 cut(s) 140, 167, 335, 602, 923, 1001, 1042, 1345, 1566
BstSLI GKGCMC 2 cut(s) 1002, 1402
BstUI CGCG 3 cut(s) 554, 970, 1832
BstV1I GCAGC 5 cut(s) 666, 673, 1074, 1124, 1846
BstZI CGGCCG 2 cut(s) 705, 732
Bsu15I ATCGAT 1 cut(s) 1479
BsuRI GGCC 9 cut(s) 146, 340, 707, 734, 887, 967, 1000, 1112, 1515
BsuTUI ATCGAT 1 cut(s) 1479
BtgI CCRYGG 1 cut(s) 1420
BtgZI GCGATG 4 cut(s) 864, 1026, 1155, 1635
BtsCI GGATG 5 cut(s) 387, 899, 1040, 1416, 1674
BveI ACCTGC 1 cut(s) 1798
Cac8I GCNNGC 7 cut(s) 99, 814, 998, 1258, 1277, 1439, 1675
CciI TCATGA 1 cut(s) 1125
CfoI GCGC 5 cut(s) 167, 818, 1801, 1834, 1869
Cfr10I RCCGGY 4 cut(s) 245, 527, 730, 812
ClaI ATCGAT 1 cut(s) 1479
CseI GACGC 6 cut(s) 172, 543, 560, 630, 769, 1065
Csp6I GTAC 3 cut(s) 1371, 1606, 1737
CspAI ACCGGT 2 cut(s) 245, 527
CviAII CATG 8 cut(s) 174, 790, 823, 1126, 1147, 1486, 1579, 1740
CviQI GTAC 3 cut(s) 1371, 1606, 1737
DdeI CTNAG 5 cut(s) 290, 489, 503, 1070, 1250
DinI GGCGCC 1 cut(s) 817
DpnI GATC 9 cut(s) 699, 776, 809, 1065, 1124, 1482, 1577, 1845, 1877
DpnII GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
DrdI GACNNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 3 cut(s) 705, 732, 885
EagI CGGCCG 2 cut(s) 705, 732
Eam1104I CTCTTC 3 cut(s) 50, 836, 1154
EarI CTCTTC 3 cut(s) 50, 836, 1154
EciI GGCGGA 2 cut(s) 401, 410
EclXI CGGCCG 2 cut(s) 705, 732
Eco130I CCWWGG 1 cut(s) 879
Eco24I GRGCYC 1 cut(s) 1002
Eco47I GGWCC 5 cut(s) 921, 945, 984, 1343, 1914
Eco52I CGGCCG 2 cut(s) 705, 732
Eco72I CACGTG 1 cut(s) 189
Eco88I CYCGRG 3 cut(s) 227, 509, 1028
Eco91I GGTNACC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 945
EcoO65I GGTNACC 1 cut(s) 211
EcoRII CCWGG 5 cut(s) 140, 335, 923, 1001, 1042
EcoT14I CCWWGG 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 1002
EgeI GGCGCC 1 cut(s) 817
EheI GGCGCC 1 cut(s) 817
ErhI CCWWGG 1 cut(s) 879
FaeI CATG 8 cut(s) 177, 793, 826, 1129, 1150, 1489, 1582, 1743
FaqI GGGAC 3 cut(s) 706, 931, 1927
FatI CATG 8 cut(s) 173, 789, 822, 1125, 1146, 1485, 1578, 1739
FauI CCCGC 6 cut(s) 400, 662, 863, 935, 989, 1666
FbaI TGATCA 1 cut(s) 1575
FokI GGATG 5 cut(s) 374, 906, 1047, 1403, 1681
FriOI GRGCYC 1 cut(s) 1002
FspBI CTAG 2 cut(s) 1385, 1928
FspI TGCGCA 1 cut(s) 1868
GlaI GCGC 5 cut(s) 166, 817, 1800, 1833, 1868
GsuI CTGGAG 1 cut(s) 644
HaeII RGCGCY 2 cut(s) 819, 1802
HaeIII GGCC 9 cut(s) 146, 340, 707, 734, 887, 967, 1000, 1112, 1515
HgaI GACGC 6 cut(s) 172, 543, 560, 630, 769, 1065
HhaI GCGC 5 cut(s) 167, 818, 1801, 1834, 1869
Hin1I GRCGYC 5 cut(s) 183, 641, 780, 816, 1076
Hin1II CATG 8 cut(s) 177, 793, 826, 1129, 1150, 1489, 1582, 1743
Hin6I GCGC 5 cut(s) 165, 816, 1799, 1832, 1867
HinP1I GCGC 5 cut(s) 165, 816, 1799, 1832, 1867
HincII GTYRAC 3 cut(s) 498, 1459, 1711
HindII GTYRAC 3 cut(s) 498, 1459, 1711
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 9 cut(s) 16, 149, 232, 253, 270, 426, 478, 512, 610
HpaI GTTAAC 3 cut(s) 498, 1459, 1711
Hpy166II GTNNAC 8 cut(s) 498, 525, 570, 1183, 1400, 1459, 1608, 1711
Hpy188I TCNGA 5 cut(s) 118, 504, 702, 774, 1094
Hpy188III TCNNGA 6 cut(s) 229, 548, 623, 1051, 1126, 1928
Hpy8I GTNNAC 8 cut(s) 498, 525, 570, 1183, 1400, 1459, 1608, 1711
Hpy99I CGWCG 7 cut(s) 210, 470, 554, 646, 706, 866, 1081
HpyAV CCTTC 3 cut(s) 1048, 1676, 1968
HpyCH4III ACNGT 3 cut(s) 286, 959, 1359
HpyCH4IV ACGT 3 cut(s) 188, 903, 1455
HpyCH4V TGCA 6 cut(s) 192, 897, 1330, 1400, 1807, 1824
HpyF3I CTNAG 5 cut(s) 290, 489, 503, 1070, 1250
HpySE526I ACGT 3 cut(s) 188, 903, 1455
Hsp92I GRCGYC 5 cut(s) 183, 641, 780, 816, 1076
Hsp92II CATG 8 cut(s) 177, 793, 826, 1129, 1150, 1489, 1582, 1743
HspAI GCGC 5 cut(s) 165, 816, 1799, 1832, 1867
KasI GGCGCC 1 cut(s) 815
KflI GGGWCCC 1 cut(s) 945
KroI GCCGGC 1 cut(s) 812
KroNI GCCGGC 1 cut(s) 814
Ksp22I TGATCA 1 cut(s) 1575
KspAI GTTAAC 3 cut(s) 498, 1459, 1711
Kzo9I GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
LmnI GCTCC 4 cut(s) 128, 383, 568, 846
Lsp1109I GCAGC 5 cut(s) 666, 673, 1074, 1124, 1846
LweI GCATC 2 cut(s) 884, 1025
MaeI CTAG 2 cut(s) 1385, 1928
MaeII ACGT 3 cut(s) 188, 903, 1455
MaeIII GTNAC 5 cut(s) 184, 211, 899, 977, 1769
MalI GATC 9 cut(s) 699, 776, 809, 1065, 1124, 1482, 1577, 1845, 1877
MbiI CCGCTC 3 cut(s) 123, 262, 939
MboI GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
MhlI GDGCHC 4 cut(s) 228, 1002, 1029, 1402
MlsI TGGCCA 1 cut(s) 887
MluCI AATT 4 cut(s) 399, 929, 1212, 1223
MluI ACGCGT 1 cut(s) 552
MluNI TGGCCA 1 cut(s) 887
Mly113I GGCGCC 1 cut(s) 816
MlyI GAGTC 4 cut(s) 264, 420, 472, 521
Mox20I TGGCCA 1 cut(s) 887
MreI CGCCGGCG 1 cut(s) 812
MroNI GCCGGC 1 cut(s) 812
MscI TGGCCA 1 cut(s) 887
MseI TTAA 6 cut(s) 497, 711, 932, 1458, 1475, 1710
MslI CAYNNNNRTG 2 cut(s) 1044, 1425
Msp20I TGGCCA 1 cut(s) 887
MspA1I CMGCKG 1 cut(s) 679
MspR9I CCNGG 9 cut(s) 142, 169, 337, 604, 925, 1003, 1044, 1347, 1568
Mva1269I GAATGC 2 cut(s) 101, 1498
MvaI CCWGG 5 cut(s) 142, 337, 925, 1003, 1044
MvnI CGCG 3 cut(s) 554, 970, 1832
NaeI GCCGGC 1 cut(s) 814
NarI GGCGCC 1 cut(s) 816
NciI CCSGG 4 cut(s) 169, 604, 1347, 1568
NdeII GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
NgoMIV GCCGGC 1 cut(s) 812
NlaIII CATG 8 cut(s) 177, 793, 826, 1129, 1150, 1489, 1582, 1743
NmeAIII GCCGAG 2 cut(s) 159, 496
NmuCI GTSAC 3 cut(s) 184, 211, 1769
NsbI TGCGCA 1 cut(s) 1868
OliI CACNNNNGTG 2 cut(s) 1044, 1425
PaeR7I CTCGAG 3 cut(s) 227, 509, 1028
PagI TCATGA 1 cut(s) 1125
PaqCI CACCTGC 1 cut(s) 1798
PasI CCCWGGG 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 3 cut(s) 194, 693, 816
PctI GAATGC 2 cut(s) 101, 1498
PdiI GCCGGC 1 cut(s) 814
PfeI GAWTC 5 cut(s) 16, 149, 232, 253, 610
PflFI GACNNNGTC 1 cut(s) 1918
PinAI ACCGGT 2 cut(s) 245, 527
PleI GAGTC 4 cut(s) 264, 420, 472, 520
PluTI GGCGCC 1 cut(s) 819
PmaCI CACGTG 1 cut(s) 189
PmlI CACGTG 1 cut(s) 189
PpsI GAGTC 4 cut(s) 264, 420, 472, 520
Ppu21I YACGTR 2 cut(s) 189, 904
PpuMI RGGWCCY 1 cut(s) 945
Psp5II RGGWCCY 1 cut(s) 945
Psp6I CCWGG 5 cut(s) 140, 335, 923, 1001, 1042
PspCI CACGTG 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 211
PspGI CCWGG 5 cut(s) 140, 335, 923, 1001, 1042
PspOMI GGGCCC 1 cut(s) 998
PspPPI RGGWCCY 1 cut(s) 945
PspXI VCTCGAGB 2 cut(s) 509, 1028
PsyI GACNNNGTC 1 cut(s) 1918
RsaI GTAC 3 cut(s) 1372, 1607, 1738
RsaNI GTAC 3 cut(s) 1371, 1606, 1737
RseI CAYNNNNRTG 2 cut(s) 1044, 1425
SaqAI TTAA 6 cut(s) 497, 711, 932, 1458, 1475, 1710
Sau3AI GATC 9 cut(s) 697, 774, 807, 1063, 1122, 1480, 1575, 1843, 1875
SchI GAGTC 4 cut(s) 264, 420, 472, 521
ScrFI CCNGG 9 cut(s) 142, 169, 337, 604, 925, 1003, 1044, 1347, 1568
SduI GDGCHC 4 cut(s) 228, 1002, 1029, 1402
SfaNI GCATC 2 cut(s) 884, 1025
SfoI GGCGCC 1 cut(s) 817
Sfr274I CTCGAG 3 cut(s) 227, 509, 1028
SgrAI CRCCGGYG 1 cut(s) 812
SinI GGWCC 5 cut(s) 921, 945, 984, 1343, 1914
SlaI CTCGAG 3 cut(s) 227, 509, 1028
SmiMI CAYNNNNRTG 2 cut(s) 1044, 1425
SmlI CTYRAG 3 cut(s) 227, 509, 1028
SmoI CTYRAG 3 cut(s) 227, 509, 1028
Sse9I AATT 4 cut(s) 399, 929, 1212, 1223
SspDI GGCGCC 1 cut(s) 815
SspMI CTAG 2 cut(s) 1385, 1928
StyD4I CCNGG 9 cut(s) 140, 167, 335, 602, 923, 1001, 1042, 1345, 1566
StyI CCWWGG 1 cut(s) 879
TaaI ACNGT 3 cut(s) 286, 959, 1359
TaiI ACGT 3 cut(s) 191, 906, 1458
TasI AATT 4 cut(s) 399, 929, 1212, 1223
TatI WGTACW 3 cut(s) 1370, 1605, 1736
TfiI GAWTC 5 cut(s) 16, 149, 232, 253, 610
Tru1I TTAA 6 cut(s) 497, 711, 932, 1458, 1475, 1710
Tru9I TTAA 6 cut(s) 497, 711, 932, 1458, 1475, 1710
TseFI GTSAC 3 cut(s) 184, 211, 1769
TseI GCWGC 6 cut(s) 4, 661, 679, 1087, 1137, 1834
Tsp45I GTSAC 3 cut(s) 184, 211, 1769
TspDTI ATGAA 3 cut(s) 1145, 1405, 1871
TspGWI ACGGA 6 cut(s) 555, 587, 906, 976, 1323, 1464
Tth111I GACNNNGTC 1 cut(s) 1918
VneI GTGCAC 1 cut(s) 1398
VpaK11BI GGWCC 5 cut(s) 921, 945, 984, 1343, 1914
XapI RAATTY 1 cut(s) 399
XbaI TCTAGA 1 cut(s) 1927
XhoI CTCGAG 3 cut(s) 227, 509, 1028
XspI CTAG 2 cut(s) 1385, 1928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.