Prupe.2G228200_v2.0.a1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0019294
Physical Location & Seq
Forward (+)
25403756 .. 25404606
851 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G228200.1

Sequence Viewer

Length: 747 bp
ATGGTTGGTTGCTTAGGCAAACTTTACGAGAGCTTCGAAAATCTCAGTGACACATACATGCAACCCAATCTTAACAAAGACTCCCTGCTGAAACCCAAGACTACAATTTCTGGCGCTAACATCCTTCATTTGCCAGCCAACAATGACTCGAATGCGCCTAAACGGTTCTACCTCTGTGCAAATTGCAAGCGCCATATTTCTGATAGTCCTTTAACAACTTGTCCAACGTGCATTTCAAGCAAAATATCCACCCAGGTGTTTTATGCTGCTCCTCCACCAGAAGCTACTGGAGTGACGTCTGGCAATATTAAGAGCGGATACGTCAAGAGCGACATTATGTACATGATAATGGATGATTTGGAAGTGAAGCCTATGCCTACTGTATCAAGTATCACATTGCTTAAAACGTTGAACTTTAAGACGGTTGATGCTTTTGAAGAGAAGGTGGTTGATCTTGACATTAAAGAGGGTTTGAAGTTGGTGAAGGCATCATTGGAGTCAAAAACCGCTCTTACAAATGTTTTCCTTGGTAATAAAGTGCATGAAGGTTTCAATTTTGGTCCCCTAAAGAAATTGAGCTTTTGGAGGTTCATTTTAGTTTGTGGCTTGGCATTAACTGTATTGGTTTACATTGGATTATTTCAACTTTGGCATACCGCAAAATATGGTAAAAACGACCCAACGAGTCCAAGGAAACCAATCTGCTATCCCAATGATCCTTTTGCACTGTCGTTGACACAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

249

Amino Acids

27.48

Weight (kDa)

8.95

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 299
AccBSI CCGCTC 2 cut(s) 315, 509
AciI CCGC 3 cut(s) 315, 507, 657
AclI AACGTT 1 cut(s) 407
AclWI GGATC 1 cut(s) 710
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 1 cut(s) 341
AgsI TTSAA 6 cut(s) 237, 412, 437, 475, 553, 644
AjnI CCWGG 1 cut(s) 252
AjuI GAANNNNNNNTTGG 4 cut(s) 217, 249, 476, 508
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 3 cut(s) 33, 284, 579
AluI AGCT 3 cut(s) 33, 284, 579
AlwI GGATC 1 cut(s) 710
ApeKI GCWGC 1 cut(s) 266
AspLEI GCGC 3 cut(s) 116, 157, 192
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 1 cut(s) 493
AsuII TTCGAA 1 cut(s) 36
AvaII GGWCC 1 cut(s) 560
BbvI GCAGC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 254
BciVI GTATCC 1 cut(s) 311
BfoI RGCGCY 2 cut(s) 117, 193
BfuI GTATCC 1 cut(s) 311
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 562
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 2 cut(s) 418, 497
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 13
Bpu14I TTCGAA 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 3 cut(s) 252, 526, 689
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bse1I ACTGG 1 cut(s) 292
Bse3DI GCAATG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 3 cut(s) 252, 526, 689
BseGI GGATG 2 cut(s) 120, 358
BseMI GCAATG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 253
BslFI GGGAC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 546
BsmI GAATGC 1 cut(s) 157
Bsp119I TTCGAA 1 cut(s) 36
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 2 cut(s) 451, 715
BspACI CCGC 3 cut(s) 315, 507, 657
BspCNI CTCAG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 562
BspPI GGATC 1 cut(s) 710
BspT104I TTCGAA 1 cut(s) 36
BsrBI CCGCTC 2 cut(s) 315, 509
BsrDI GCAATG 1 cut(s) 395
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 1 cut(s) 292
BssECI CCNNGG 3 cut(s) 252, 526, 689
BssMI GATC 2 cut(s) 451, 715
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 2 cut(s) 526, 689
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 5 cut(s) 165, 382, 424, 619, 729
Bst6I CTCTTC 1 cut(s) 432
BstACI GRCGYC 1 cut(s) 296
BstAUI TGTACA 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 135, 188
BstDEI CTNAG 2 cut(s) 13, 44
BstF5I GGATG 2 cut(s) 120, 358
BstH2I RGCGCY 2 cut(s) 117, 193
BstHHI GCGC 3 cut(s) 116, 157, 192
BstKTI GATC 2 cut(s) 454, 718
BstMBI GATC 2 cut(s) 451, 715
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 253
BsuI GTATCC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 120, 358
BtsIMutI CAGTG 2 cut(s) 52, 725
Cac8I GCNNGC 2 cut(s) 135, 188
CfoI GCGC 3 cut(s) 116, 157, 192
Cfr13I GGNCC 1 cut(s) 560
Csp6I GTAC 1 cut(s) 340
CviAII CATG 3 cut(s) 58, 343, 542
CviJI RGCY 6 cut(s) 33, 137, 284, 370, 579, 606
CviKI_1 RGCY 6 cut(s) 33, 137, 284, 370, 579, 606
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 2 cut(s) 13, 44
DpnI GATC 2 cut(s) 453, 717
DpnII GATC 2 cut(s) 451, 715
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Eco130I CCWWGG 2 cut(s) 526, 689
Eco47I GGWCC 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 2 cut(s) 526, 689
ErhI CCWWGG 2 cut(s) 526, 689
FaeI CATG 3 cut(s) 61, 346, 545
FaqI GGGAC 1 cut(s) 546
FatI CATG 3 cut(s) 57, 342, 541
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 2 cut(s) 107, 365
Fsp4HI GCNGC 1 cut(s) 267
GlaI GCGC 3 cut(s) 115, 156, 191
GluI GCNGC 1 cut(s) 267
GsuI CTGGAG 1 cut(s) 309
HaeII RGCGCY 2 cut(s) 117, 193
HhaI GCGC 3 cut(s) 116, 157, 192
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 3 cut(s) 61, 346, 545
Hin6I GCGC 3 cut(s) 114, 155, 190
HinP1I GCGC 3 cut(s) 114, 155, 190
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 4 cut(s) 80, 146, 497, 685
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 2 cut(s) 628, 735
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 2 cut(s) 325, 455
Hpy8I GTNNAC 2 cut(s) 628, 735
HpyAV CCTTC 4 cut(s) 134, 436, 478, 539
HpyCH4III ACNGT 5 cut(s) 165, 382, 424, 619, 729
HpyCH4IV ACGT 4 cut(s) 227, 296, 321, 407
HpyCH4V TGCA 6 cut(s) 61, 179, 186, 231, 541, 725
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpyF3I CTNAG 2 cut(s) 13, 44
HpySE526I ACGT 4 cut(s) 227, 296, 321, 407
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 3 cut(s) 61, 346, 545
HspAI GCGC 3 cut(s) 114, 155, 190
Kzo9I GATC 2 cut(s) 451, 715
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 8 cut(s) 96, 98, 147, 239, 266, 273, 285, 291
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 2 cut(s) 418, 497
MaeII ACGT 4 cut(s) 227, 296, 321, 407
MaeIII GTNAC 2 cut(s) 47, 292
MalI GATC 2 cut(s) 453, 717
MbiI CCGCTC 2 cut(s) 315, 509
MboI GATC 2 cut(s) 451, 715
MboII GAAGA 1 cut(s) 449
MluCI AATT 4 cut(s) 105, 181, 553, 572
MlyI GAGTC 4 cut(s) 74, 140, 506, 694
MmeI TCCRAC 1 cut(s) 248
MnlI CCTC 4 cut(s) 182, 282, 460, 579
MseI TTAA 7 cut(s) 72, 212, 309, 402, 417, 462, 614
MslI CAYNNNNRTG 4 cut(s) 56, 254, 347, 742
MspR9I CCNGG 1 cut(s) 254
Mva1269I GAATGC 1 cut(s) 157
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 237
NdeII GATC 2 cut(s) 451, 715
NlaIII CATG 3 cut(s) 61, 346, 545
NlaIV GGNNCC 1 cut(s) 562
NmuCI GTSAC 2 cut(s) 47, 292
NspI RCATGY 1 cut(s) 61
NspV TTCGAA 1 cut(s) 36
OliI CACNNNNGTG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 327
PctI GAATGC 1 cut(s) 157
PkrI GCNGC 1 cut(s) 268
PleI GAGTC 4 cut(s) 74, 140, 505, 693
PpsI GAGTC 4 cut(s) 74, 140, 505, 693
Psp1406I AACGTT 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 562
PspPI GGNCC 1 cut(s) 560
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 4 cut(s) 56, 254, 347, 742
SaqAI TTAA 7 cut(s) 72, 212, 309, 402, 417, 462, 614
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 2 cut(s) 451, 715
Sau96I GGNCC 1 cut(s) 560
SchI GAGTC 4 cut(s) 74, 140, 506, 694
ScrFI CCNGG 1 cut(s) 254
SfaNI GCATC 2 cut(s) 418, 497
SfuI TTCGAA 1 cut(s) 36
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 4 cut(s) 56, 254, 347, 742
Sse9I AATT 4 cut(s) 105, 181, 553, 572
SsiI CCGC 3 cut(s) 315, 507, 657
SspI AATATT 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 2 cut(s) 526, 689
TaaI ACNGT 5 cut(s) 165, 382, 424, 619, 729
TaiI ACGT 4 cut(s) 230, 299, 324, 410
TaqI TCGA 2 cut(s) 36, 149
TasI AATT 4 cut(s) 105, 181, 553, 572
TatI WGTACW 1 cut(s) 339
Tru1I TTAA 7 cut(s) 72, 212, 309, 402, 417, 462, 614
Tru9I TTAA 7 cut(s) 72, 212, 309, 402, 417, 462, 614
TscAI CASTG 2 cut(s) 52, 732
TseFI GTSAC 2 cut(s) 47, 292
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 2 cut(s) 47, 292
TspDTI ATGAA 3 cut(s) 116, 558, 580
TspRI CASTG 2 cut(s) 52, 732
VpaK11BI GGWCC 1 cut(s) 560
XceI RCATGY 1 cut(s) 61
ZraI GACGTC 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.